Bacterium inoculation method and special-purpose ejecting type separating gun for the same

A bacterial inoculation and spraying technology, applied in the direction of bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of complicated operation, inappropriate bacteria that are not resistant to high temperature, easy to cause pollution, etc., to achieve easy dispersion, Easy to operate and reduce the chance of bacterial loss

Inactive Publication Date: 2008-09-10
蒲鹏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When this method is used to separate bacteria, since the culture medium (mostly containing agar) must remain liquid before pouring, the temperature must of course be higher than 60°C, so it is not suitable for bacteria that are not resistant to high temperatures, and the operation is complicated and easy to cause pollution

Method used

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  • Bacterium inoculation method and special-purpose ejecting type separating gun for the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A bacterial inoculation method, the method is to use the principle of the suction ear ball to uniformly spray the bacterial solution into the culture medium through the power of the instantaneously generated high-pressure gas.

Embodiment 2

[0022] The following combination figure 1 A kind of jet type separation gun of the present invention is described in further detail, comprises a rubber ball 1 and nozzle 7, is characterized in that, described rubber ball 1 is connected with one end of connecting pipe 2 sealingly, and the other end of connecting pipe 2 is connected with One end of the connecting pipe 6 is sealed and connected, and the other end of the connecting pipe 6 is connected with the nozzle 7;

[0023] A gas filter 3 is connected to the side wall of the connecting pipe 2, and an air intake valve 4 is connected to the gas filter 3;

[0024] A hole 8 is provided at one end of the connecting pipe 2 connected to the connecting pipe 3 , and an outlet valve 5 is connected at a position corresponding to the hole 8 .

[0025] The intake valve 4 is a one-way valve.

[0026] The air outlet valve 5 is a one-way valve.

[0027] The other end of the nozzle 7 is a bell mouth.

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Abstract

The invention discloses a bacterium inoculation method, which utilizes the principle of a rubber pipette bulb to uniformly spray the force of high-pressure gas generated instantly into a culture medium. The invention also discloses a special jet type separation gun, which comprises a rubber ball and a nozzle, wherein, the rubber ball is airtightly connected with one end of a connecting pipe; the other end of the connecting pipe is airtightly connected with one end of another connecting pipe; the other end of the other connecting pipe is connected with the nozzle; a gas filter is arranged on the sidewall of the connecting pipe; an inlet valve is connected to the gas filter; a hole is arranged on the end where the connecting pipe is connected with the other connecting pipe; an outlet valve is arranged on a position opposite to the hole; and the other end of the nozzle takes the shape of a bell mouth. The bacterial separation gun makes bacteria easier to be dispersed and can well separate bacteria which are not high-temperature resistant; moreover, the operation is simple and convenient and the steps for a bacterium sample to be polluted are reduced.

Description

technical field [0001] The invention relates to an inoculation method in biological cultivation, and also relates to a spray type separation gun used for inoculation by the method. Background technique [0002] The current bacterial isolation methods include platinum bait needle drawing line inoculation and pouring plate method. When the former method is used to take bacterial samples, it is very suitable for colony samples, but it is easy to miss some bacteria for liquid samples, resulting in the loss of target bacteria. The latter method is a method in which a certain amount of bacterial liquid sample is taken with a pipette or a syringe, added to a plate, and then poured into the culture medium for cultivation. When this method is used to separate bacteria, since the culture medium (mostly containing agar) must remain in a liquid state before pouring, the temperature must of course be higher than 60°C, so it is not suitable for bacteria that are not resistant to high tem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12M1/26C12R1/01
CPCC12M29/14C12M33/04C12M29/04C12M29/06
Inventor 蒲鹏
Owner 蒲鹏
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