170 results about "Sterile environment" patented technology

The invention is applicable to the technical field of biotechnology and genetic engineering, and provides an agrobacterium rhizogenes mediated genetic transformation method for hairy roots of blueberries. According to the method, a blueberry perennial stem segment is used as a receptor material for infection, and rapid genetic transformation of the blueberry stem segment is realized through an agrobacterium tumefaciens-mediated method. The method ingeniously bypasses the lengthy process of differentiating the explants to form somatic embryos, and compared with a traditional leaf disc method genetic transformation system, the method has the advantage that the genetic transformation period is remarkably shortened. And the method can be used for mass infection. Even in a non-sterile environment, the method can realize high-efficiency transformation of the gene, and shows the characteristics of simplicity and high efficiency. Therefore, the invention not only provides a new way for genetic transformation of the blueberries, but also provides valuable reference for genetic transformation research and gene function research of the blueberries.

The invention discloses a sludge treatment method and system, and relates to the field of solid waste treatment, and the method specifically comprises the following operation steps: S1, multi-frequency resonance pretreatment: destroying a sludge colloid structure through stepped mechanical vibration: collapsing an extracellular polymer network at a low frequency, breaking down microbial cell walls at an intermediate frequency to release intracellular water, pathogenic bacteria are inactivated through a high-frequency cavitation effect; s2, performing electrochemical in-situ flocculation; s3, gradient pressure dehydration; s4, low-temperature mineralization stabilization; and S5, vacuum condensation and recovery. According to the sludge treatment method and system, through stepped low-frequency, medium-frequency and high-frequency mechanical resonance, the colloid structure of the sludge can be directionally damaged, the fluidity of the sludge is remarkably improved, chemical conditioners are remarkably reduced, agent residues and secondary pollution are avoided, pathogens are physically inactivated through the high-frequency cavitation effect, and the treatment effect of the sludge is improved. Micro-nano oxygen bubbles are synchronously generated to strengthen free radical oxidation, and a sterile environment is created for subsequent mineralization.

The invention provides application of a bacteriostatic agent in open type tissue culture and rapid propagation of polyploidy bamboo reeds, and relates to the technical field of tissue culture and rapid propagation of plants, the bacteriostatic agent is applied to the open type tissue culture and rapid propagation process of the polyploidy bamboo reeds, and the use method is that the bacteriostatic agent is added into a culture medium for use or sprayed or oscillated and flushed for use, and the bacteriostatic agent is applied to the open type tissue culture and rapid propagation process of the polyploidy bamboo reeds. The bacteriostatic agent is formed by mixing isothiazolinone and sodium dehydroacetate, the mass addition ratio of the isothiazolinone to the sodium dehydroacetate is (8-5): (2-5), and the method comprises the following steps: S1, primary culture of explants; s2, multiplication culture of seedlings; s3, rooting culture, wherein each liter of the culture medium contains 0.05-1g of the bacteriostatic agent in the above steps. The method has the beneficial effects that under the condition that the method does not need to be controlled in a sterile environment and does not depend on the quality of an inoculator, a large amount of propagation of the polyploidy bamboo reed tissue culture seedlings is realized by adopting an open tissue culture technical mode, the productivity speed of a tissue culture center is improved, and the construction threshold and cost of the tissue culture center are reduced.

According to the compound quaternary ammonium salt disinfectant for the sterile environment and the preparation method thereof, the benzalkonium chloride, the polyhexamethylene biguanide hydrochloride and the plant polysaccharide are compounded to form the positively charged plant polysaccharide compound disinfectant under the action of the sodium sulfite or the potassium sulfite by utilizing a compound synergistic principle, so that the compound quaternary ammonium salt disinfectant for the sterile environment is obtained. Positive charges are promoted to penetrate through cell walls of bacterial biofilms, so that an excellent bactericidal effect is achieved; the sodium hyaluronate, the enhancer and the glycerol are cooperatively used, so that sodium ions are combined with dehydrogenation groups in polysaccharide and hydroxyl groups, carboxyl groups and the like of other components to form a relatively stable internal net-shaped structure, and the situation that quaternary ammonium salt reacts with metal ions in the environment to reduce the disinfection effect is effectively avoided; meanwhile, the sodium hyaluronate and the glycerol are used for enhancing the compatibility of all the components, and the moisturizing effect of the components is fully utilized, so that the hand sanitizer is not only suitable for disinfection of object surfaces and space environments in a sterile environment, but also can be used for disinfection of hands of a human body.

The invention belongs to the field of microbial agents, and particularly relates to a microbial composite agent for degrading herbicides and a preparation method of the microbial composite agent. The preparation method of the microbial complex microbial inoculant comprises the following preparation steps: mixing bran hydrolysate, peat soil and diatomite, sterilizing, adding a protective agent and a complex flora suspension in a sterile environment, uniformly mixing, kneading and forming, preparing into slices, naturally air-drying, and crushing to obtain the microbial inoculant, and uniformly mixing the microbial agent with the adsorbent, the surfactant and the nutrient substances to obtain the microbial complex microbial inoculant. According to the invention, domesticated microorganisms form a composite flora to prepare the microbial agent, and the composite flora exerts unique degradation capability and generates synergistic effect, so that the herbicide can be degraded more comprehensively and efficiently, and the proportion of each microorganism, an adsorbent, a surfactant and other components in the microbial agent can be adjusted according to different herbicide types and polluted environments; and efficient degradation of specific herbicides is realized.

The invention provides a regulation and control method for prolonging the refreshing time of prunes, which comprises the following steps: disinfecting a phase-temperature fresh-keeping storehouse for 24 hours by using absolute ethyl alcohol, and ventilating for 8-10 hours to ensure a sterile environment. During harvesting, prunes which are free of plant diseases and insect pests, free of mechanical damage, complete in fruit cream and larger than or equal to 20% in sugar content are selected, and the temperature of the prunes is rapidly reduced to 2-2.2 DEG C within 10 hours by means of portable differential pressure precooling equipment. After precooling, a methyl jasmonate aqueous solution is adopted for electrostatic atomization spraying, and 1-methylcyclopropene is used for airtight fumigation for 18-20 hours to inhibit ethylene generation and respiration. And then, storing in a phase temperature warehouse at the temperature of-1.5 to-1.2 DEG C, controlling the relative humidity to be 85-87%, and carrying out intermittent nano-atomization sterilization by using a hydrogen peroxide aqueous solution once every 15-18 days. According to the method, the fresh-keeping effect of the prunes is comprehensively improved, the safety and the quality of the fruits are ensured, and the fresh-keeping period is remarkably prolonged.

The invention relates to the technical field of biopharmaceutical equipment, in particular to a sterile culture method and a sterile culture system. The sterile culture system comprises a cell preparation device, an incubator and a cell culture device, the cell preparation device comprises a preparation chamber, and a first sealing valve is arranged on the side wall of the preparation chamber; the incubator is provided with a culture chamber, a second sealing valve is arranged on the side wall of the culture chamber, and the second sealing valve is used for being matched with the first sealing valve, so that the incubator and the cell preparation device are in sealed butt joint or sealed separation; the cell culture device is used for accommodating the culture box, supplying power and gas to the culture box and controlling the cell growth environment in the culture box. As the cells always grow in the sterile environment, the pollution risk is low, frequent sterilization is not needed, the normal growth of the cells is prevented from being influenced by frequent sterilization, the culture time is saved, and the cell preparation and culture efficiency is high.

A preparation method of a packaging material comprises the following steps: selecting at least one of sawdust, rice husk, corn straw and wheat straw, crushing to obtain raw material powder, then adding a carbon source, a nitrogen source and inorganic salt, and uniformly mixing to obtain a matrix; the prepared matrix is sterilized, and the sterilized matrix is obtained; in a sterile environment, cooling the sterilized substrate, and inoculating an edible fungus strain; putting the matrix inoculated with the edible fungus strain into a constant-temperature and constant-humidity culture room, and culturing for 10-20 days; putting the mycelium obtained by culturing and a substrate into a pre-designed mold together for molding; drying the formed mycelium material in drying equipment at 40-60 DEG C until the water content is reduced to be less than 10%; and carrying out surface treatment on the dried mycelium material. The obtained packaging material is completely biodegradable, harmful substances are not generated in the production process, and the current environmental protection requirement is met.

Systems, devices, and methods for producing a tissue specimen and performing subsequent biomechanical testing thereof to quantify various biological / physiological properties and characteristics. The system may include a bioreactor device for producing the tissue specimen which includes a packaged / sealed seeding cup containing a scaffold in a sterile environment amenable to seeding with cells. After seeding, the cup may be stored in a standard laboratory culture tray for incubation. The produced tissue specimen may next be transferred to a testing instrument under control of a microcontroller which automatically performs a series of tests on the tissue and generates testing data communicated to an external electronic device which can operably interact with and control operation in part of the testing instrument via software. Tissue integrity in the instrument may be maintained by sterile fluid circulation and heating systems. The system advantageously provides efficient and reproducible tissue growth and analysis.

The invention provides a regulation and control method for prolonging the refreshing time of prunes, which comprises the following steps: disinfecting a phase-temperature fresh-keeping storehouse for 24 hours by using absolute ethyl alcohol, and ventilating for 8-10 hours to ensure a sterile environment. During harvesting, prunes which are free of plant diseases and insect pests, free of mechanical damage, complete in fruit cream and larger than or equal to 20% in sugar content are selected, and the temperature of the prunes is rapidly reduced to 2-2.2 DEG C within 10 h through portable differential pressure precooling equipment. After precooling, a methyl jasmonate aqueous solution is adopted for electrostatic atomization spraying, and 1-methylcyclopropene is used for airtight fumigation for 18-20 h to inhibit ethylene generation and respiration. And then, storing in a phase temperature warehouse at the temperature of-1.5 to-1.2 DEG C, controlling the relative humidity to be 85-87%, and carrying out intermittent nano atomization sterilization by using a hydrogen peroxide aqueous solution once every 15-18 days. According to the method, the fresh-keeping effect of the prunes is comprehensively improved, the safety and the quality of the fruits are ensured, and the fresh-keeping period is remarkably prolonged.

The present invention belongs to the field of natural compounds, and specifically relates to a crude extract of frog follicles, the VMO1 protein, and their extraction methods and uses. The specific technical solution is as follows: A crude extract, wherein the crude extract is from frog follicles and does not contain egg grains. The preparation method includes: extracting the follicles of frogs, removing the egg grains under a sterile environment, adding a buffer solution, performing ultrasonic treatment and centrifugation, taking the supernatant, and filtering to obtain the crude extract. The crude extract obtained by the present invention has good maintained activity and can be further developed for subsequent uses. The crude extract obtained by the present invention can significantly reduce the surface tension, and at the same time has good antioxidant activity and good antibacterial ability, such as the ability to inhibit Pseudomonas aeruginosa. Since products derived from natural products have good biocompatibility, therefore, based on the above activities and functions, the crude extract provided by the present invention has good application prospects in multiple fields such as the field of cosmetics water retention, the field of food preservation, and the field of pharmaceuticals.

The utility model relates to the technical field of fungus stick inoculation machine provides a fungus stick inoculation machine, including frame, inoculation device, blanking device, eye -boring device, stop device and spray brush sterilization device, and set up in the air purifier and distribution box of frame top, fungus stick removes and sends device and is arranged in the frame inside, the inoculation device includes setting on fungus stick removes and sends device inoculation stick and the blanking device of located inoculation stick top, and inoculation stick removes and send in proper order through spray brush sterilization device, eye -boring device and inoculation device. Through the spray brush sterilization device, eye -boring device, inoculation device and fungus stick removes and send device are integrated in same frame, realize fungus stick disinfection, drilling, inoculation's full process automation continuous operation. Spray brush sterilization device in real time disinfects surface before fungus stick enters drilling station, and the clean air is continuously conveyed to the top operating area in combination with air purifier, and the sterile environment is double -guaranteed.

The utility model provides a strain fermentation tank inoculation device which comprises a barrel and a flame ring, the barrel can be arranged on an inoculation opening in a sleeving mode, a sleeve is fixed to a fermentation tank, a net ring is fixed to the inner wall of the barrel, the flame ring is annularly fixed to the upper end of the net ring, and a conical cover integrally formed with the barrel is arranged at the upper end of the barrel. The top of the conical cover is provided with a through hole capable of pouring bacterial liquid for inoculation through the conical flask. According to the inoculation device for the strain fermentation tank, when a flame inoculation method is used for strain inoculation, flames generated by igniting the flame ring are limited in the barrel, the effects that the flames cover the inoculation opening to keep a sterile environment and prevent infectious microbes from entering the fermentation tank are achieved, diffusion of surrounding flames is limited through the barrel and the conical cover, and the inoculation effect is improved. And the risk that operators are scalded is reduced.

The invention discloses a microalgae culture device capable of realizing self-adaptive supply of dissolved oxygen by using a waterproof breathable membrane tube, a large number of micropores with pore diameters smaller than those of microalgae cells and bacteria are distributed on the tube wall of the waterproof breathable membrane tube, and the waterproof breathable membrane tube can effectively prevent bacteria in air from entering a fermentation tank, ensure a sterile environment in the tank and improve the utilization rate of the microalgae. Microalgae cells can be prevented from entering the membrane tube to cause cell loss; meanwhile, the wall surface of the waterproof and breathable membrane tube has hydrophobicity, a liquid culture medium can be effectively prevented from entering the membrane tube, a gas supply channel in the membrane tube is prevented from being blocked, and the tube wall of the waterproof and breathable membrane tube only allows gas molecules such as oxygen and carbon dioxide to freely permeate; after the air pump pressurizes ambient air, the ambient air is injected into the waterproof breathable membrane tube from the air inlet of the fermentation tank, and oxygen in the gas in the waterproof breathable membrane tube penetrates through the tube wall under the driving of concentration gradient to enter the suspension type microalgae culture system so as to provide oxygen for heterotrophic growth of microalgae cells.

The invention provides a granular fat low-temperature collecting device for fat transplantation, and relates to the technical field of fat transplantation. The sealing mechanism is fixedly mounted in the storage mechanism; the top of the automatic blocking mechanism is inserted into the storage mechanism and is communicated with the sealing mechanism; the top of the fat granule docking mechanism is connected with the automatic blocking mechanism; the auxiliary cleaning mechanism is mounted at the top of the storage mechanism; during butt joint conveying, injection of excess air can be greatly reduced; the collection and storage barrel is independently arranged in the storage mechanism; butt joint conveying can be matched, and automatic opening, closing and blocking can be carried out; internal cleaning of the multiple sets of collection and storage barrels can be completed rapidly and conveniently; the problems that if air in an internal area of a bearing container contains bacteria or other substances, fat granules can be influenced to a certain extent, and the temperature and the sterile environment in a heat preservation box can be influenced to a certain extent due to frequent opening, closing and taking are solved.

The utility model relates to the technical field of biopharmaceutical equipment, in particular to a sterile culture system. The sterile culture system comprises a cell preparation device, an incubator and a cell culture device, the cell preparation device comprises a preparation chamber, and a first sealing valve is arranged on the side wall of the preparation chamber; the incubator is provided with a culture chamber, a second sealing valve is arranged on the side wall of the culture chamber, and the second sealing valve is used for being matched with the first sealing valve, so that the incubator and the cell preparation device are in sealed butt joint or sealed separation; the cell culture device is used for accommodating the culture box, supplying power and gas to the culture box and controlling the cell growth environment in the culture box. As the cells always grow in the sterile environment, the pollution risk is low, frequent sterilization is not needed, the normal growth of the cells is prevented from being influenced by frequent sterilization, the culture time is saved, and the cell preparation and culture efficiency is high.

The invention relates to the technical field of stem cell preparation, and provides a sterile operation device for stem cell preparation, comprising a base and an operation box fixedly mounted on the base, wherein an inlet and outlet groove is provided at the bottom of one end of the base, a rising groove communicating with the inlet and outlet groove is provided at the middle position of the top of the base, an inlet and outlet communicating with the rising groove is provided at the middle position of the bottom of the operation box, a side groove is provided at one end of the rising groove, and a ventilation cavity is provided inside the top corner of the base; a placing frame is arranged to fit inside the inlet and outlet groove. The invention ensures that the operation area is in a sterile environment, is convenient for taking and placing stem cell storage containers, is convenient for replacing sterile air in a small ventilation area, avoids the problem of long-term contact and contamination of stem cells with bacteria, and hinders the entry of sterile air into the operation device, avoids the contact of stem cells with bacteria at the first time.

The invention relates to the technical field of food processing, and particularly discloses a processing method for preparing sea cucumber viscera into powder. The raw materials of the product comprise fresh sea cucumber viscera and composite auxiliary materials, wherein the composite auxiliary materials comprise marine fish collagen peptide and euphausia superba powder; the preparation method comprises the following steps: pretreating fresh sea cucumbers, taking out viscera, cleaning, mixing the viscera with composite auxiliary materials, pulping, filtering to obtain composite juice, carrying out ultrasonic-assisted enzymatic degradation on the composite juice, inactivating bio-enzyme, carrying out low-temperature concentration and drying to obtain primary powder, carrying out non-thermal sterilization and high-pressure sterilization on the primary powder, and packaging to obtain the sea cucumber beverage. And cooling and filling in a sterile environment. The product can be used as a functional food raw material, and has the advantages of reservation of heat-sensitive active ingredients such as sea cucumber saponin and stable quality; in addition, the preparation method disclosed by the invention has the advantages of efficiently utilizing byproducts of the internal organs of the sea cucumbers, being mild in process, not destroying active ingredients and controllable in operation, and resource waste and active ingredient loss can be reduced.

The application belongs to the technical field of tissue culture and relates to a kind of induction method and application of monkey ear ring flower filament callus, comprising the following steps: taking monkey ear ring flower bud, after being treated with disinfecting liquid for disinfection and sterilization, in sterile environment, take monkey ear ring flower filament as explant, it is inoculated in induction medium and dark culture, form callus, then the callus is transferred to proliferation medium and is subcultured and proliferated, and more high-quality callus is obtained.The induction method provided by the application can induce monkey ear ring flower filament to form callus quickly, and the callus induction rate is as high as 100%;The induction method of monkey ear ring flower filament callus in the application has short cultivation period, high induction efficiency and low contamination rate, the induction method is applied to the cultivation of monkey ear ring, the research path of monkey ear ring rapid propagation technology is widened, the regeneration of monkey ear ring flower filament into plant becomes possible, and high-quality plant with excellent traits can be cultivated.

The utility model discloses a phellinus igniarius sterile culture dish convenient to lock, and relates to the technical field of culture dishes. The device comprises a bottom cover used for culturing phellinus igniarius; the top cover is used for being matched with the bottom cover to provide a sterile environment, and the inner wall of the top cover is in sliding connection with the outer wall of the bottom cover; the culture dish further comprises a locking device and a lantern ring, the locking device is used for fixing the bottom cover and the top cover, the outer wall of the locking device is in sliding connection with the inner wall of the bottom cover, the lantern ring is used for enabling the inner wall of the lantern ring to be in sliding connection with the outer wall of the top cover, and by arranging the locking device, after the bottom cover and the top cover of the culture dish are sleeved with each other, the bottom cover and the top cover can be fixed. According to the culture dish locking device, locking can be conducted, the culture dish can be fixed and locked, meanwhile, the fixing step of the culture dish is simplified, meanwhile, the unlocking step is easy, and the purpose of saving the operation time of a user is achieved.

The utility model relates to the technical field of fermentation tanks, and discloses a fermentation tank which comprises a tank body, a steam conveying pipe is connected to the top of the tank body, an air filter is connected to one end of the steam conveying pipe, a liquid outlet is formed in the bent position of the bottom of the steam conveying pipe, and a first connecting pipe is connected to the liquid outlet of the bottom of the steam conveying pipe. One end of the first connecting pipe is connected with a second connecting pipe through a connecting assembly, and one end of the second connecting pipe is connected with an exhaust valve. According to the fermentation tank, condensate water formed in a steam conveying pipe can flow into a first connecting pipe from a liquid discharging opening in the bent position of the bottom of the steam conveying pipe, and then is discharged from an exhaust valve in cooperation with a second connecting pipe, so that the condensate water deposited at the bent position of the bottom of the steam conveying pipe can be discharged; a large number of bacteria are prevented from entering the fermentation tank along with the steam pipeline due to deposition of condensed water, and the effect of a dry and sterile environment at the bent part of the bottom of the steam pipeline can be ensured.

The utility model provides an aloe gel extraction device based on a sterile environment, which relates to the technical field of plant processing and comprises an extraction tank, a filter cartridge is arranged in the extraction tank, the outlet end of the filter cartridge is connected with a filter pipe, the tail end in the extraction tank is connected with a filter plate, a stirring component is arranged in the filter cartridge, and filter holes are arrayed on the surface of the filter cartridge circumferentially. The top end of the extraction tank is provided with a feeding end, the surface of the filter cartridge is sleeved with a sealing cover, the sealing cover is movably connected with the surface of the filter cartridge, opening grooves are formed in the surface of the sealing cover in a circumferential array mode, and the filter cartridge, the filter pipe and the filter plate are all installed in the extraction tank, so that mixing and filtering operation can be carried out on the same equipment; and vacuum treatment is carried out during mixing, so that treatment in a sterile environment is achieved.

The invention relates to a plant cultivation method, in particular to a sterile miniature cuttage rapid propagation method for brassica napobrassica. According to the method, stem segments with buds or terminal buds of the brassica napobrassica are used as explants for asexual propagation, budlet micro cutting is performed in a sterile environment, plantlets with roots are directly induced without adding any plant hormones and inducing cluster buds and calluses, and rapid propagation of the brassica napobrassica is realized through continuous micro cutting. The method is not limited by seasons and propagation materials, seedlings can be produced in a manual control environment all the year round, the propagation speed is high, and the coefficient is large.

The utility model discloses an active medical device. The active medical equipment comprises a body which is provided with a heat dissipation channel, and the heat dissipation channel penetrates through the body in the first direction; a sealing cavity is formed in the body, the sealing cavity surrounds the heat dissipation channel, and the sealing cavity and the heat dissipation channel are isolated from each other; and the functional device is arranged in the sealing cavity. The active medical equipment provided by the embodiment of the utility model can effectively reduce the pollution of dust and bacteria in the bacteria environment to the sterile environment in the process of alternately using in the bacteria environment and the sterile environment.

The invention relates to the related technical field of culture medium preparation, discloses an automatic preparation device for a microbial culture medium, and aims to solve the problem of component pollution caused by exposure of the culture medium in the external environment in the preparation process. The medicine feeding cylinder is used for penetrating into the injection plug, dropper feeding is replaced with an injection mode, external environment pollution is avoided, and meanwhile accurate feeding can be achieved through scales on the medicine feeding cylinder. After all the components of the culture medium are injected, a motor is used for driving the central disc to rotate, so that the bevel gear on the central disc rotates along the fixed gear ring, the culture medium test tube fixed on the bevel gear continuously rotates, and all the components in the culture medium test tube are mixed; finally, the effects of batching in a sterile environment and rotary stirring of the test tube are achieved.

The present application relates to the technical field of bacterial storage, and particularly relates to a bacterial drug resistance detection storage device. The present application provides a bacterial drug resistance detection storage device which can effectively reduce bacterial infection and more quickly drip wax on a bacterial culture dish. The bacterial drug resistance detection storage device comprises a storage cabinet, a cabinet door, a placing table and a reciprocating moving mechanism, etc. The storage cabinet is divided into a main storage chamber and a sub-storage chamber. The cabinet door is connected to one side of the storage cabinet near the sub-storage chamber through a hinge. The placing table is placed on the bottom of one side of the main storage chamber in the storage cabinet. The reciprocating moving mechanism is arranged on one side of the main storage chamber in the storage cabinet. The motor drives the lead screw to rotate, and then drives the disc to rotate, opens the intermittent liquid feeding pipe, and the alcohol in the intermittent liquid feeding pipe flows to the four rotating liquid spraying pipes, and is uniformly sprayed out through the plurality of spray holes on the rotating liquid spraying pipes, so as to sterilize and disinfect the sub-storage chamber and the placing table of the storage cabinet, and more ensure the sterile environment when dripping wax on the bacterial culture dish.

The invention relates to the technical field of microorganism culture, in particular to a ganoderma sinensis spore germination culture medium and application thereof.Ganoderma sinensis spores are collected through a fungus carrying bag or the culture medium, the optimal growth environment for ganoderma sinensis spore germination culture is obtained by optimizing the culture condition of the ganoderma sinensis spores, and the ganoderma sinensis spore germination culture medium is obtained by optimizing the culture medium formula and adding wood chip boiling liquid. A culture medium formula most suitable for ganoderma spore germination is obtained through raw material screening and orthogonal experiment optimization of boiling liquid, ganoderma spores can be effectively collected without reaching a sterile environment of a laboratory, the method is a practical and effective ganoderma sinensis spore collection and germination method, and a foundation is laid for promoting ganoderma sinensis new variety breeding research.

The utility model relates to the technical field of sterilization equipment, in particular to a water bath sterilization all-in-one machine which comprises a supporting frame and a supporting disc, a conveying belt is arranged at the top of the supporting frame, the supporting disc is arranged at the top of the conveying belt, filling bottles are movably connected into the supporting disc, and connecting frames are fixedly connected to the two sides of the top of the supporting frame and located above the conveying belt. Five heating water outlet nozzles are fixedly connected to one side in the connecting frame at equal intervals, water blowing pipes are fixedly connected to the positions, located on one sides of the heating water outlet nozzles, in the connecting frame, water blowing heads are fixedly connected to the bottoms of the water blowing pipes, and sterilizing lamps are fixedly connected to the positions, located on the sides, away from the heating water outlet nozzles, of the bottoms of the inner walls of the connecting frame. The high-temperature water bath sterilization device has the beneficial effects that the high-temperature water bath can ensure that the temperature and the time required by sterilization are reached, microorganisms such as bacteria, viruses and spores are effectively killed, residual moisture on the surface or inside a sterilized article is avoided, the possibility of secondary pollution is reduced, and a sterile environment is ensured.

The invention relates to a method for increasing the sporulation of bacteria such as Bacillus velezensis and Paenibacillus polymyxa using a liquid medium comprising glucose, ammonium sulfate, magnesium chloride and non-ionic surfactant polymer compounds. This method for increasing sporulation is used in a method for generating a large quantity of spores and selecting spores more resistant to higher temperatures than the spores of wild strains. The spores with higher heat resistance than those of the wild strains can be used in a method for eliminating contamination in fermentations.

The invention discloses a clean bench of a sterile filling machine, and relates to the technical field of filling machines, the clean bench comprises a support, a filling bench is arranged in the support, a base is fixedly arranged at the bottom of the filling bench, the support is fixedly connected to the bottom of the base, a T-shaped cylinder is fixedly arranged at the top of the filling bench, a clamping device is fixedly arranged on the inner side wall of the T-shaped cylinder, and a filling port and a capping device are slidably arranged at the top of the support. Overflowed water flows into the water collecting tank through the filter screen, in order to avoid bacterium breeding caused by accumulated water and ensure that the clean bench is in a sterile environment, the clean bench is sterilized through the ultraviolet lamp, and the ultraviolet lamp can sterilize flowing water and prevent bacterium breeding in the water collecting tank; the filter screen is fixed through the bolts, the filter screen can be quickly disassembled only by disassembling the bolts during disassembly, and the device not only can prevent water accumulation, but also can ensure that no bacteria breed, and makes up for the defects of an existing device.