90 results about "Sterile environment" patented technology

The invention discloses a sludge treatment method and system, and relates to the field of solid waste treatment, and the method specifically comprises the following operation steps: S1, multi-frequency resonance pretreatment: destroying a sludge colloid structure through stepped mechanical vibration: collapsing an extracellular polymer network at a low frequency, breaking down microbial cell walls at an intermediate frequency to release intracellular water, pathogenic bacteria are inactivated through a high-frequency cavitation effect; s2, performing electrochemical in-situ flocculation; s3, gradient pressure dehydration; s4, low-temperature mineralization stabilization; and S5, vacuum condensation and recovery. According to the sludge treatment method and system, through stepped low-frequency, medium-frequency and high-frequency mechanical resonance, the colloid structure of the sludge can be directionally damaged, the fluidity of the sludge is remarkably improved, chemical conditioners are remarkably reduced, agent residues and secondary pollution are avoided, pathogens are physically inactivated through the high-frequency cavitation effect, and the treatment effect of the sludge is improved. Micro-nano oxygen bubbles are synchronously generated to strengthen free radical oxidation, and a sterile environment is created for subsequent mineralization.

The invention provides application of a bacteriostatic agent in open type tissue culture and rapid propagation of polyploidy bamboo reeds, and relates to the technical field of tissue culture and rapid propagation of plants, the bacteriostatic agent is applied to the open type tissue culture and rapid propagation process of the polyploidy bamboo reeds, and the use method is that the bacteriostatic agent is added into a culture medium for use or sprayed or oscillated and flushed for use, and the bacteriostatic agent is applied to the open type tissue culture and rapid propagation process of the polyploidy bamboo reeds. The bacteriostatic agent is formed by mixing isothiazolinone and sodium dehydroacetate, the mass addition ratio of the isothiazolinone to the sodium dehydroacetate is (8-5): (2-5), and the method comprises the following steps: S1, primary culture of explants; s2, multiplication culture of seedlings; s3, rooting culture, wherein each liter of the culture medium contains 0.05-1g of the bacteriostatic agent in the above steps. The method has the beneficial effects that under the condition that the method does not need to be controlled in a sterile environment and does not depend on the quality of an inoculator, a large amount of propagation of the polyploidy bamboo reed tissue culture seedlings is realized by adopting an open tissue culture technical mode, the productivity speed of a tissue culture center is improved, and the construction threshold and cost of the tissue culture center are reduced.

The utility model relates to the technical field of fungus stick inoculation machine provides a fungus stick inoculation machine, including frame, inoculation device, blanking device, eye -boring device, stop device and spray brush sterilization device, and set up in the air purifier and distribution box of frame top, fungus stick removes and sends device and is arranged in the frame inside, the inoculation device includes setting on fungus stick removes and sends device inoculation stick and the blanking device of located inoculation stick top, and inoculation stick removes and send in proper order through spray brush sterilization device, eye -boring device and inoculation device. Through the spray brush sterilization device, eye -boring device, inoculation device and fungus stick removes and send device are integrated in same frame, realize fungus stick disinfection, drilling, inoculation's full process automation continuous operation. Spray brush sterilization device in real time disinfects surface before fungus stick enters drilling station, and the clean air is continuously conveyed to the top operating area in combination with air purifier, and the sterile environment is double -guaranteed.

The invention discloses a microalgae culture device capable of realizing self-adaptive supply of dissolved oxygen by using a waterproof breathable membrane tube, a large number of micropores with pore diameters smaller than those of microalgae cells and bacteria are distributed on the tube wall of the waterproof breathable membrane tube, and the waterproof breathable membrane tube can effectively prevent bacteria in air from entering a fermentation tank, ensure a sterile environment in the tank and improve the utilization rate of the microalgae. Microalgae cells can be prevented from entering the membrane tube to cause cell loss; meanwhile, the wall surface of the waterproof and breathable membrane tube has hydrophobicity, a liquid culture medium can be effectively prevented from entering the membrane tube, a gas supply channel in the membrane tube is prevented from being blocked, and the tube wall of the waterproof and breathable membrane tube only allows gas molecules such as oxygen and carbon dioxide to freely permeate; after the air pump pressurizes ambient air, the ambient air is injected into the waterproof breathable membrane tube from the air inlet of the fermentation tank, and oxygen in the gas in the waterproof breathable membrane tube penetrates through the tube wall under the driving of concentration gradient to enter the suspension type microalgae culture system so as to provide oxygen for heterotrophic growth of microalgae cells.

The invention relates to the technical field of food processing, and particularly discloses a processing method for preparing sea cucumber viscera into powder. The raw materials of the product comprise fresh sea cucumber viscera and composite auxiliary materials, wherein the composite auxiliary materials comprise marine fish collagen peptide and euphausia superba powder; the preparation method comprises the following steps: pretreating fresh sea cucumbers, taking out viscera, cleaning, mixing the viscera with composite auxiliary materials, pulping, filtering to obtain composite juice, carrying out ultrasonic-assisted enzymatic degradation on the composite juice, inactivating bio-enzyme, carrying out low-temperature concentration and drying to obtain primary powder, carrying out non-thermal sterilization and high-pressure sterilization on the primary powder, and packaging to obtain the sea cucumber beverage. And cooling and filling in a sterile environment. The product can be used as a functional food raw material, and has the advantages of reservation of heat-sensitive active ingredients such as sea cucumber saponin and stable quality; in addition, the preparation method disclosed by the invention has the advantages of efficiently utilizing byproducts of the internal organs of the sea cucumbers, being mild in process, not destroying active ingredients and controllable in operation, and resource waste and active ingredient loss can be reduced.

The application belongs to the technical field of tissue culture and relates to a kind of induction method and application of monkey ear ring flower filament callus, comprising the following steps: taking monkey ear ring flower bud, after being treated with disinfecting liquid for disinfection and sterilization, in sterile environment, take monkey ear ring flower filament as explant, it is inoculated in induction medium and dark culture, form callus, then the callus is transferred to proliferation medium and is subcultured and proliferated, and more high-quality callus is obtained.The induction method provided by the application can induce monkey ear ring flower filament to form callus quickly, and the callus induction rate is as high as 100%;The induction method of monkey ear ring flower filament callus in the application has short cultivation period, high induction efficiency and low contamination rate, the induction method is applied to the cultivation of monkey ear ring, the research path of monkey ear ring rapid propagation technology is widened, the regeneration of monkey ear ring flower filament into plant becomes possible, and high-quality plant with excellent traits can be cultivated.

The utility model relates to the technical field of fermentation tanks, and discloses a fermentation tank which comprises a tank body, a steam conveying pipe is connected to the top of the tank body, an air filter is connected to one end of the steam conveying pipe, a liquid outlet is formed in the bent position of the bottom of the steam conveying pipe, and a first connecting pipe is connected to the liquid outlet of the bottom of the steam conveying pipe. One end of the first connecting pipe is connected with a second connecting pipe through a connecting assembly, and one end of the second connecting pipe is connected with an exhaust valve. According to the fermentation tank, condensate water formed in a steam conveying pipe can flow into a first connecting pipe from a liquid discharging opening in the bent position of the bottom of the steam conveying pipe, and then is discharged from an exhaust valve in cooperation with a second connecting pipe, so that the condensate water deposited at the bent position of the bottom of the steam conveying pipe can be discharged; a large number of bacteria are prevented from entering the fermentation tank along with the steam pipeline due to deposition of condensed water, and the effect of a dry and sterile environment at the bent part of the bottom of the steam pipeline can be ensured.

The invention relates to a plant cultivation method, in particular to a sterile miniature cuttage rapid propagation method for brassica napobrassica. According to the method, stem segments with buds or terminal buds of the brassica napobrassica are used as explants for asexual propagation, budlet micro cutting is performed in a sterile environment, plantlets with roots are directly induced without adding any plant hormones and inducing cluster buds and calluses, and rapid propagation of the brassica napobrassica is realized through continuous micro cutting. The method is not limited by seasons and propagation materials, seedlings can be produced in a manual control environment all the year round, the propagation speed is high, and the coefficient is large.

The present application relates to the technical field of bacterial storage, and particularly relates to a bacterial drug resistance detection storage device. The present application provides a bacterial drug resistance detection storage device which can effectively reduce bacterial infection and more quickly drip wax on a bacterial culture dish. The bacterial drug resistance detection storage device comprises a storage cabinet, a cabinet door, a placing table and a reciprocating moving mechanism, etc. The storage cabinet is divided into a main storage chamber and a sub-storage chamber. The cabinet door is connected to one side of the storage cabinet near the sub-storage chamber through a hinge. The placing table is placed on the bottom of one side of the main storage chamber in the storage cabinet. The reciprocating moving mechanism is arranged on one side of the main storage chamber in the storage cabinet. The motor drives the lead screw to rotate, and then drives the disc to rotate, opens the intermittent liquid feeding pipe, and the alcohol in the intermittent liquid feeding pipe flows to the four rotating liquid spraying pipes, and is uniformly sprayed out through the plurality of spray holes on the rotating liquid spraying pipes, so as to sterilize and disinfect the sub-storage chamber and the placing table of the storage cabinet, and more ensure the sterile environment when dripping wax on the bacterial culture dish.

The utility model relates to the technical field of sterilization equipment, in particular to a water bath sterilization all-in-one machine which comprises a supporting frame and a supporting disc, a conveying belt is arranged at the top of the supporting frame, the supporting disc is arranged at the top of the conveying belt, filling bottles are movably connected into the supporting disc, and connecting frames are fixedly connected to the two sides of the top of the supporting frame and located above the conveying belt. Five heating water outlet nozzles are fixedly connected to one side in the connecting frame at equal intervals, water blowing pipes are fixedly connected to the positions, located on one sides of the heating water outlet nozzles, in the connecting frame, water blowing heads are fixedly connected to the bottoms of the water blowing pipes, and sterilizing lamps are fixedly connected to the positions, located on the sides, away from the heating water outlet nozzles, of the bottoms of the inner walls of the connecting frame. The high-temperature water bath sterilization device has the beneficial effects that the high-temperature water bath can ensure that the temperature and the time required by sterilization are reached, microorganisms such as bacteria, viruses and spores are effectively killed, residual moisture on the surface or inside a sterilized article is avoided, the possibility of secondary pollution is reduced, and a sterile environment is ensured.

The invention relates to a method for increasing the sporulation of bacteria such as Bacillus velezensis and Paenibacillus polymyxa using a liquid medium comprising glucose, ammonium sulfate, magnesium chloride and non-ionic surfactant polymer compounds. This method for increasing sporulation is used in a method for generating a large quantity of spores and selecting spores more resistant to higher temperatures than the spores of wild strains. The spores with higher heat resistance than those of the wild strains can be used in a method for eliminating contamination in fermentations.

The invention belongs to the field of natural compounds, and particularly relates to a frog follicle crude extract and an extraction method and application thereof. The specific technical scheme is as follows: the crude extract is from frog follicles of rana poxing and does not contain egg granules. The preparation method comprises the following steps: extracting follicles of frogs, removing egg granules in a sterile environment, adding a buffer solution, carrying out ultrasonic treatment, centrifuging, taking supernatant, and filtering to obtain the crude extract. The crude extract obtained by the invention keeps good activity, and can be further developed for subsequent application. The crude extract obtained by the invention can obviously reduce the surface tension, and has better antioxidant activity at the same time. As a natural product source product has good biocompatibility, the crude extract provided by the invention has good application prospects in multiple fields such as the cosmetic water retention field, the food preservation field and the pharmaceutical field based on the activity and the function.

The invention provides an open type tissue culture method of bamboo reed, which comprises the following tissue culture steps: S1, preparation of a gradient antibacterial culture medium: (1) preparation of the gradient antibacterial culture medium: an MS culture medium, 0.5 mg / L 6-BA and 0.1 mg / L NAA are added, 0.1% of tea polyphenol aqueous extract is added, and 0.03% of water-soluble chitosan oligosaccharide is added. Traditional chemical disinfection is replaced with a natural gradient antibacterial system, the dual functions of pollution prevention and control and root protection are achieved by combining a symbiotic bacterium slow release technology, and the stress resistance and lignification characteristics of plants are synchronously improved through the synergistic effect of salinity gradient stimulation and environmental signal molecules; a light-gas intelligent regulation and control system is developed to simulate the optimal growth condition, and a transplanting survival guarantee system is constructed in cooperation with a mycorrhiza pre-activation technology; the dependence of traditional tissue culture on a sterile environment is broken through, and the seedling culture efficiency, the ecological compatibility and the resource utilization level are comprehensively improved under an open condition.

The invention discloses a lucid ganoderma cultivation method which comprises the following steps: S1, preparation of a culture medium: weighing coffee grounds, tea leaf residues, bran, sawdust, perlite, charcoal, cane sugar and gypsum, and uniformly stirring the components in proportion; s2, sterilization: performing normal-pressure sterilization; s3, sterile inoculation: uniformly spreading strains on the surface of the culture medium in a sterile environment; s4, mycelium culture management: keeping the temperature, the air humidity and the ventilation quantity of a culture room, and culturing in a dark place; s5, management in a sporocarp formation period: when a small amount of brown primordia appear, improving air humidity, illumination intensity and ventilation quantity for culture; and S6, harvesting sporocarp. According to the ganoderma lucidum cultivation method, sufficient and appropriate nutrients can be provided for growth of ganoderma lucidum, the breathability of the culture medium is improved, hypha growth is accelerated, and the ganoderma lucidum yield is increased; the adsorption of autotoxic substances generated by continuous cropping of the ganoderma lucidum is enhanced, the influence of heavy metals on the ganoderma lucidum is reduced, the cultivation environment is improved, and the quality of the ganoderma lucidum is improved.

The invention relates to the technical field of mushroom stick inoculation machines, and provides a mushroom stick inoculation machine which comprises a rack, an inoculation device, a discharging device, a drilling device, a limiting device, a spraying disinfection device, an air purifier, a distribution box and a mushroom stick transferring device, the inoculating device comprises an inoculating stick arranged on the mushroom stick transferring device and a discharging device located at the top of the inoculating stick, and the inoculating stick is transferred and sequentially passes through the spraying and brushing disinfection device, the drilling device and the inoculating device. The spraying disinfection device, the drilling device, the inoculation device and the mushroom stick transferring device are cooperatively integrated on the same rack, so that full-process automatic continuous operation of mushroom stick disinfection, drilling and inoculation is realized. The spraying disinfection device disinfects the surfaces of the mushroom sticks in real time before the mushroom sticks enter the drilling station, clean air is continuously conveyed to the top operation area in combination with the air purifier, and the sterile environment is doubly guaranteed.

The invention relates to a plant, which can be operated in a plurality of operating modes, for filling objects in a sterile environment, said plant comprising a filling station, a station arranged downstream of the filling station, a freeze dryer, a first transport device (2) for transporting objects from the filling station towards the second station, a second transport device (3) for transporting objects to the freeze dryer, and a transfer device (4) arranged at a junction point between the first transport device (2) and the second transport device (3), wherein, in a first operating mode, objects can be transported from the filling station to the freeze dryer, in a second operating mode, objects can be transported from the freeze dryer towards the second station, and in a third operating mode, objects can be transported from the filling station towards the second station, wherein the first transport device comprises a linear transport path (20), wherein the junction point is provided on the linear transport path (20), wherein the transfer device (4) can be configured selectively in a rest state and in at least one working state, wherein, in the rest state, objects can be transported by means of the first transport device (2) such that the objects pass the junction point and, while passing the junction point, remain within the first transport device (2). The invention also relates to a method for filling and sealing objects.

This invention discloses a loose mulberry leaf bud Fu brick tea and its preparation method. The method comprises: picking mulberry leaf buds, spreading them out, fixing them, and drying them to make mulberry leaf bud tea; extracting, filtering, and concentrating Anhua black tea to make a concentrated liquid; mixing 100 parts of mulberry leaf bud tea with 20-50 parts of the concentrated liquid, adjusting the moisture content of the mulberry leaf bud tea, and then piling it up; after drying, wrapping it in a sterile environment and then treating it at a short time with high temperature; mixing Fu brick tea with sterile water and boiling it three times, the first two times at 70-85℃ and the third time at 90-100℃, filtering it in a clean environment after each boiling, and continuing to boil the residue; combining the filtrates and concentrating them under reduced pressure to make a glaze; piling the glaze with the short-time high-temperature treated mulberry leaf bud tea in a sterile environment at 15-25℃ and 20%-60% humidity, then fermenting it in an environment with 50%-80% relative humidity for 7-12 days, and then drying it to obtain loose mulberry leaf bud Fu brick tea.

The invention discloses an open-type cold region Chinese rose tissue culture seedling propagation method, which is characterized in that parachlorometaxylenol and miconazole nitrate are used as bacteriostatic agents and are respectively applied to tissue culture links such as explant earlier-stage treatment, appliance sterilization, culture medium preparation, explant disinfection, tissue culture seedling transplanting and the like, so that the propagation process of the cold region Chinese rose tissue culture seedling gets rid of environmental limitation. Explant disinfection does not need a strict sterile environment, a high-pressure sterilization pot and an ultra-clean workbench are not used, and rapid breeding of Chinese rose tissue culture seedlings is achieved. Compared with a conventional sterilization method, the induction rate of the cluster buds is remarkably improved, and the pollution rate, the vitrification rate and the browning rate are remarkably reduced. The growth vigor of the tissue culture seedlings after rooting is obviously improved; and particularly, the effects of Hanjin No.1 and Changbai Mountain vine roses are prominent.

The invention relates to the technical field of efficient culture of microorganisms, in particular to an efficient culture device of microorganisms, which comprises a culture tank body, a pouring-in pipe arranged on the culture tank body and a sealing cover arranged on the pouring-in pipe, and a guide-in shell is arranged on the outer side of the pouring-in pipe. The guiding-in shell is arranged on the sealing cover, the putting-in opening where the fire ring can be conveniently placed is formed in the guiding-in shell, then the fire ring can be placed at the guiding-in shell, the uncovering mechanism is arranged on the guiding-in shell, then the effect of opening the sealing cover is achieved, and when the sealing cover is opened, the microorganism pouring mechanism is arranged, so that the fire ring can be conveniently poured out. Therefore, the bottle opening of the culture bottle filled with microorganisms can be slowly rotated to the fire ring, meanwhile, the bottle plug is opened, efficient inoculation in a sterile environment is achieved, meanwhile, through high-precision cooperation of a plurality of mechanisms, the phenomena that the bottle plug is opened too slowly or the dumping angle is improper and the like are avoided, and the inoculation efficiency is improved. The effect of efficiently culturing the microorganisms is further realized.

The application relates to the technical field of medical auxiliary devices, in particular to a blood bag warming device for a blood transfusion department, which comprises a sterilization box, a box cover, an electric push rod one, a heating assembly, a disturbance assembly and a pump suction assembly. The electric push rod one is arranged in the sterilization box and connected with the output end of the box cover. The bottom of the box cover is provided with two mounting racks. The bottom of each of the two mounting racks is provided with an electric push rod two. The heating assembly comprises two clamping plates, and the two clamping plates are arranged on the corresponding mounting racks. The disturbance assembly is provided with two and connected with the output end of the corresponding electric push rod two. The heating assembly, the disturbance assembly and the pump suction assembly can realize synchronous dynamic warming of the two sides of the blood bag in a sterile environment, improve the warming efficiency and avoid the breeding of bacteria to affect the treatment effect.

The invention relates to the technical field of cell separation, and particularly discloses a cell separation device which comprises a main body, the rotating platform is arranged in the main body, and the rotating platform rotates at a high speed to generate centrifugal force, so that target cells are separated from other components; the number of the bases is four, and the four bases are distributed in an annular array mode relative to the center axis of the rotating platform; the first reset spring provides upward elastic force for the sliding block, the second reset spring provides downward elastic force for the clamping seat, and when the centrifugal tube moves along with the clamping seat, the first reset spring simultaneously absorbs power of the clamping seat, protects the centrifugal tube, gently and uniformly mixes, prevents cells from settling to block a filter membrane, but avoids generation of foam; four centrifugal tubes are uniformly mixed in the rotating platform at the same time, so that the experiment process is reduced, a sample is uniformly mixed in a sterile environment, the possibility that the sample is polluted by microorganisms outside the sterile environment is reduced, and the accuracy and the treatment effect of a subsequent experiment are influenced.

The application discloses an indoor seedling raising method of Tricholoma mongolicum, and relates to the technical field of indoor seedling raising, which comprises the following steps: preparing a low-nutrient symbiotic germination culture medium, taking water and agar as the basis, containing 0-5 g / L sugar substances and humus soil extract, and forming a sterile environment beneficial to the symbiosis of fungi; selecting associated fungi to prepare mycelium fragment suspension as symbiotic inoculants through liquid fermentation; performing surface disinfection and sterile ultrasonic treatment on Tricholoma mongolicum seeds; sowing the seeds on the surface of the culture medium and inoculating the symbiotic inoculants under sterile conditions, and culturing under artificial climate control of temperature and light, so as to promote the symbiotic relationship between the fungi and the seeds; and obtaining symbiotic seedlings after the seeds swell and differentiate into protocorms or young leaves. The symbiotic germination mechanism of associated fungi is introduced into the indoor sterile culture system, the emergence rate can reach more than 90%, the emergence uniformity is high, the seedlings are not limited by seasons and climate, the seedlings are self-brought mycorrhizal roots, the transplanting survival rate is high, and the reliable seedling guarantee is provided for the large-scale artificial planting of Tricholoma mongolicum.

A device for providing a sterile environment for the incubation of cell cultures is proposed, comprising a warming chamber with heating elements, sensors for temperature, humidity and CO2, and a gas supply providing a gas connection in the interior. The device also has a cultivation tank located in the warming chamber. This has a sealable passage for each sensor, and also a sealable gas inlet and outlet that couples to the gas connection inside the warming chamber. The sensors of the warming chamber are arranged to fit into respective passages of the cultivation vessel. The device also has a water reservoir arranged in the cultivation tank. The three elements of the warming chamber, the cultivation container and the water reservoir can be sterilized separately using the optimal method for each. Since the sensors are not part of the cultivation container, the latter can also be decontaminated by high temperatures. The sterilized warming chamber ensures that the cell cultures in the cultivation container cannot be contaminated from the outside, nor can they cause contamination in the laboratory.

The invention relates to the technical field of experimental animals, in particular to a hatching method of bacteria-limited chickens and application of the bacteria-limited chickens, and the hatching method comprises the following steps: disinfecting the surfaces of SPF hatching eggs by using alcohol with the volume fraction of 60-80% and ultraviolet rays every day during hatching, and disinfecting by using a formaldehyde fumigation method every other week. According to the method provided by the invention, a sterile environment in the hatching process is created through disinfection at a specific time, and the SPF hatching eggs in the hatching process are disinfected, so that the breeding of microorganisms on the surfaces of eggshells is inhibited, the microbial pollution in the exchange process of chick embryos and external environment substances is reduced to a great extent, and the survival rate of the chick embryos is increased. The bacterium-limited chicken with beneficial bacteria such as rumen bacillus, enterococcus, bacillus terminus and proteophilic bacteria is obtained, and no Chryseobacterium exists in the bacterium-limited chicken.

The utility model discloses a sterilization device for a feed microorganism bacterial liquid delivery pipeline, which comprises a plurality of seed tanks and a plurality of inoculation tanks, the seed tanks and the plurality of inoculation tanks are communicated through liquid delivery pipes and branch pipes, the branch pipes are communicated with a medium outlet end of the sterilization device, and the sterilization device comprises a drying mechanism, a steam mechanism, a main pipe and an auxiliary pipe. The medium outlet end of the drying mechanism is communicated with the main pipe through the auxiliary pipe, the medium outlet end of the steam mechanism is communicated with the main pipe, the medium outlet end of the main pipe is communicated with the branch pipe, and the branch pipe, the main pipe and the auxiliary pipe are all provided with electromagnetic valves. According to the device, two different media can be sequentially introduced into the infusion tube according to the sequence, high-temperature steam is firstly introduced for sterilization, then dry air with temperature is introduced for heating residual water vapor so as to ensure dryness in the infusion tube, the infusion tube is treated by using the device, the sterile environment of the infusion tube is ensured, and the safety of the infusion tube is ensured. And the concentration of the strain is not influenced.

The utility model discloses a 50L bacitracin zinc fermentation tank inoculation tool, and belongs to the technical field of biological strain inoculation. Comprising an inoculating loop, an adjusting base and a windproof assembly, the adjusting base is rotationally installed at the bottom of the inoculating loop, an alcohol cotton groove with an opening in the top is formed between the adjusting base and the inoculating loop, the windproof assembly is vertically and slidably arranged in the alcohol cotton groove in a covering mode, abutting rods are symmetrically installed at the top of the adjusting base, and the abutting rods are matched with the windproof assembly in an abutting mode. The inner wall of the inoculating loop is fixedly connected with an arc-shaped track for the abutting rod to move, and an outlet is formed in one end of the arc-shaped track. By means of alcohol cotton burning in the alcohol cotton groove, the pollution risk in the inoculation process is effectively reduced, the sterile environment in the fermentation process is guaranteed, the inoculation operation is more flexible due to the design of the adjusting base and the arc-shaped track, the windproof assembly can be rapidly lifted by screwing the adjusting base, and the stability of flame burning is enhanced.

The present application discloses a filling device, comprising a sterile chamber, a clamping component, a transmission component, and a power component, and further comprising a steam barrier chamber. The clamping component is located in the sterile chamber, the power component is located outside the sterile chamber and the steam barrier chamber, and the transmission component passes through the steam barrier chamber to be connected to the clamping component and the power component. In the present application, the clamping component which can be thoroughly sterilized is fully arranged in the sterile chamber, and the power component which is difficult to thoroughly sterilize is arranged outside the sterile chamber and is driven by means of the transmission component, so that the power component can stably drive the clamping component in the sterile chamber outside the sterile chamber; the steam barrier chamber can prevent bacteria in external air from entering the sterile chamber, and can also sterilize, by means of high-temperature steam, the transmission component passing through the steam barrier chamber, so as to prevent the transmission component from carrying bacteria from the outside into the sterile chamber during movement, so that the sterile chamber can maintain a sterile environment for a long time, thereby ensuring stable sterile filling.

The present invention discloses a cordyceps flower production and cultivation system based on a strain fermentation optimization method, which relates to the technical field of cordyceps flower production and cultivation, and includes: a supporting component, a cultivation component, an opening component, a supply component, a support component, a drive component and a spraying component, wherein the supporting component is used for bottom support. In the present invention, under the condition that the cultivation box is not opened or punched, the strain spraying and inoculation operation of the strain on the culture medium is realized only through the center hole position, the degree of automation is high, and under the condition that the cultivation box is not opened or punched, the impact on the cultivation environment inside the cultivation box is small, avoiding the sterile environment of the culture medium being affected during the inoculation operation, and at the same time, the nozzle gradually moves toward the outer circle to rotate and spray, which can achieve comprehensive and uniform coverage of the culture medium by strain spraying, and has a high utilization efficiency of the culture medium. The strains at each position can absorb sufficient nutrients, which is conducive to the efficient cultivation of cordyceps flowers.

This invention provides a sugarcane tissue culture seedling cultivation system, belonging to the field of sugarcane cultivation technology. It includes a sterile environment cultivation chamber, an environmental factor synergistic regulation system, a machine vision-based intelligent management system for culture media and cultures, culture bottles, and culture racks. The culture bottles are placed on the culture racks, which are located within the sterile environment cultivation chamber. This invention establishes a light-temperature-humidity-air linkage model to simulate the optimal growth microenvironment, significantly promoting the robust growth of sugarcane tissue culture seedlings, shortening the cultivation cycle, and increasing the proliferation coefficient and rooting rate. It utilizes machine vision technology to automatically monitor growth parameters such as seedling height, bud number, and root system, accurately determining the optimal time for transfer and termination of rooting culture, eliminating weak, deformed, and contaminated seedlings, and greatly improving the uniformity and quality of seedlings. By optimizing the design of the lighting system and the standardized placement of culture bottles, it ensures uniform light distribution and reduces energy consumption; through precise control, it reduces resource waste.

The invention discloses a boletus aereus solid strain culture method, and relates to the technical field of microbial culture, the method comprises the following steps: preparing a special culture medium taking hard broadleaf sawdust with specific particle size and corncob as main materials, wheat bran and soybean meal as auxiliary materials, and mulberry twig fermentation dry powder as a biological activity induction component; putting the special solid culture medium into a culture bottle, sealing, sterilizing, and cooling for later use; inoculating the boletus aereus mother strain into the sterilized special solid culture medium; a micro-positive pressure intermittent ventilation culture process which is closed in the whole process and does not need punching is adopted for the culture bottle after culture and inoculation. The invention aims to solve the problems of high pollution risk, slow hypha growth and high planting failure rate caused by manual punching due to compact structure and poor air permeability of the existing solid culture gene, realizes the cooperation of high-porosity oxygen supply, precise nutrition supply and sterile environment guarantee, and remarkably improves the hypha activity and culture success rate. The method is suitable for efficient industrial production of boletus aereus solid strains.