50 results about "Sterile environment" patented technology

The utility model relates to the technical field of fungus stick inoculation machine provides a fungus stick inoculation machine, including frame, inoculation device, blanking device, eye -boring device, stop device and spray brush sterilization device, and set up in the air purifier and distribution box of frame top, fungus stick removes and sends device and is arranged in the frame inside, the inoculation device includes setting on fungus stick removes and sends device inoculation stick and the blanking device of located inoculation stick top, and inoculation stick removes and send in proper order through spray brush sterilization device, eye -boring device and inoculation device. Through the spray brush sterilization device, eye -boring device, inoculation device and fungus stick removes and send device are integrated in same frame, realize fungus stick disinfection, drilling, inoculation's full process automation continuous operation. Spray brush sterilization device in real time disinfects surface before fungus stick enters drilling station, and the clean air is continuously conveyed to the top operating area in combination with air purifier, and the sterile environment is double -guaranteed.

The invention discloses a microalgae culture device capable of realizing self-adaptive supply of dissolved oxygen by using a waterproof breathable membrane tube, a large number of micropores with pore diameters smaller than those of microalgae cells and bacteria are distributed on the tube wall of the waterproof breathable membrane tube, and the waterproof breathable membrane tube can effectively prevent bacteria in air from entering a fermentation tank, ensure a sterile environment in the tank and improve the utilization rate of the microalgae. Microalgae cells can be prevented from entering the membrane tube to cause cell loss; meanwhile, the wall surface of the waterproof and breathable membrane tube has hydrophobicity, a liquid culture medium can be effectively prevented from entering the membrane tube, a gas supply channel in the membrane tube is prevented from being blocked, and the tube wall of the waterproof and breathable membrane tube only allows gas molecules such as oxygen and carbon dioxide to freely permeate; after the air pump pressurizes ambient air, the ambient air is injected into the waterproof breathable membrane tube from the air inlet of the fermentation tank, and oxygen in the gas in the waterproof breathable membrane tube penetrates through the tube wall under the driving of concentration gradient to enter the suspension type microalgae culture system so as to provide oxygen for heterotrophic growth of microalgae cells.

The invention relates to the technical field of food processing, and particularly discloses a processing method for preparing sea cucumber viscera into powder. The raw materials of the product comprise fresh sea cucumber viscera and composite auxiliary materials, wherein the composite auxiliary materials comprise marine fish collagen peptide and euphausia superba powder; the preparation method comprises the following steps: pretreating fresh sea cucumbers, taking out viscera, cleaning, mixing the viscera with composite auxiliary materials, pulping, filtering to obtain composite juice, carrying out ultrasonic-assisted enzymatic degradation on the composite juice, inactivating bio-enzyme, carrying out low-temperature concentration and drying to obtain primary powder, carrying out non-thermal sterilization and high-pressure sterilization on the primary powder, and packaging to obtain the sea cucumber beverage. And cooling and filling in a sterile environment. The product can be used as a functional food raw material, and has the advantages of reservation of heat-sensitive active ingredients such as sea cucumber saponin and stable quality; in addition, the preparation method disclosed by the invention has the advantages of efficiently utilizing byproducts of the internal organs of the sea cucumbers, being mild in process, not destroying active ingredients and controllable in operation, and resource waste and active ingredient loss can be reduced.

The application belongs to the technical field of tissue culture and relates to a kind of induction method and application of monkey ear ring flower filament callus, comprising the following steps: taking monkey ear ring flower bud, after being treated with disinfecting liquid for disinfection and sterilization, in sterile environment, take monkey ear ring flower filament as explant, it is inoculated in induction medium and dark culture, form callus, then the callus is transferred to proliferation medium and is subcultured and proliferated, and more high-quality callus is obtained.The induction method provided by the application can induce monkey ear ring flower filament to form callus quickly, and the callus induction rate is as high as 100%;The induction method of monkey ear ring flower filament callus in the application has short cultivation period, high induction efficiency and low contamination rate, the induction method is applied to the cultivation of monkey ear ring, the research path of monkey ear ring rapid propagation technology is widened, the regeneration of monkey ear ring flower filament into plant becomes possible, and high-quality plant with excellent traits can be cultivated.

The utility model relates to the technical field of fermentation tanks, and discloses a fermentation tank which comprises a tank body, a steam conveying pipe is connected to the top of the tank body, an air filter is connected to one end of the steam conveying pipe, a liquid outlet is formed in the bent position of the bottom of the steam conveying pipe, and a first connecting pipe is connected to the liquid outlet of the bottom of the steam conveying pipe. One end of the first connecting pipe is connected with a second connecting pipe through a connecting assembly, and one end of the second connecting pipe is connected with an exhaust valve. According to the fermentation tank, condensate water formed in a steam conveying pipe can flow into a first connecting pipe from a liquid discharging opening in the bent position of the bottom of the steam conveying pipe, and then is discharged from an exhaust valve in cooperation with a second connecting pipe, so that the condensate water deposited at the bent position of the bottom of the steam conveying pipe can be discharged; a large number of bacteria are prevented from entering the fermentation tank along with the steam pipeline due to deposition of condensed water, and the effect of a dry and sterile environment at the bent part of the bottom of the steam pipeline can be ensured.

The invention relates to a plant cultivation method, in particular to a sterile miniature cuttage rapid propagation method for brassica napobrassica. According to the method, stem segments with buds or terminal buds of the brassica napobrassica are used as explants for asexual propagation, budlet micro cutting is performed in a sterile environment, plantlets with roots are directly induced without adding any plant hormones and inducing cluster buds and calluses, and rapid propagation of the brassica napobrassica is realized through continuous micro cutting. The method is not limited by seasons and propagation materials, seedlings can be produced in a manual control environment all the year round, the propagation speed is high, and the coefficient is large.

The present application relates to the technical field of bacterial storage, and particularly relates to a bacterial drug resistance detection storage device. The present application provides a bacterial drug resistance detection storage device which can effectively reduce bacterial infection and more quickly drip wax on a bacterial culture dish. The bacterial drug resistance detection storage device comprises a storage cabinet, a cabinet door, a placing table and a reciprocating moving mechanism, etc. The storage cabinet is divided into a main storage chamber and a sub-storage chamber. The cabinet door is connected to one side of the storage cabinet near the sub-storage chamber through a hinge. The placing table is placed on the bottom of one side of the main storage chamber in the storage cabinet. The reciprocating moving mechanism is arranged on one side of the main storage chamber in the storage cabinet. The motor drives the lead screw to rotate, and then drives the disc to rotate, opens the intermittent liquid feeding pipe, and the alcohol in the intermittent liquid feeding pipe flows to the four rotating liquid spraying pipes, and is uniformly sprayed out through the plurality of spray holes on the rotating liquid spraying pipes, so as to sterilize and disinfect the sub-storage chamber and the placing table of the storage cabinet, and more ensure the sterile environment when dripping wax on the bacterial culture dish.

The utility model relates to the technical field of sterilization equipment, in particular to a water bath sterilization all-in-one machine which comprises a supporting frame and a supporting disc, a conveying belt is arranged at the top of the supporting frame, the supporting disc is arranged at the top of the conveying belt, filling bottles are movably connected into the supporting disc, and connecting frames are fixedly connected to the two sides of the top of the supporting frame and located above the conveying belt. Five heating water outlet nozzles are fixedly connected to one side in the connecting frame at equal intervals, water blowing pipes are fixedly connected to the positions, located on one sides of the heating water outlet nozzles, in the connecting frame, water blowing heads are fixedly connected to the bottoms of the water blowing pipes, and sterilizing lamps are fixedly connected to the positions, located on the sides, away from the heating water outlet nozzles, of the bottoms of the inner walls of the connecting frame. The high-temperature water bath sterilization device has the beneficial effects that the high-temperature water bath can ensure that the temperature and the time required by sterilization are reached, microorganisms such as bacteria, viruses and spores are effectively killed, residual moisture on the surface or inside a sterilized article is avoided, the possibility of secondary pollution is reduced, and a sterile environment is ensured.

The invention relates to a method for increasing the sporulation of bacteria such as Bacillus velezensis and Paenibacillus polymyxa using a liquid medium comprising glucose, ammonium sulfate, magnesium chloride and non-ionic surfactant polymer compounds. This method for increasing sporulation is used in a method for generating a large quantity of spores and selecting spores more resistant to higher temperatures than the spores of wild strains. The spores with higher heat resistance than those of the wild strains can be used in a method for eliminating contamination in fermentations.

The invention relates to the technical field of efficient culture of microorganisms, in particular to an efficient culture device of microorganisms, which comprises a culture tank body, a pouring-in pipe arranged on the culture tank body and a sealing cover arranged on the pouring-in pipe, and a guide-in shell is arranged on the outer side of the pouring-in pipe. The guiding-in shell is arranged on the sealing cover, the putting-in opening where the fire ring can be conveniently placed is formed in the guiding-in shell, then the fire ring can be placed at the guiding-in shell, the uncovering mechanism is arranged on the guiding-in shell, then the effect of opening the sealing cover is achieved, and when the sealing cover is opened, the microorganism pouring mechanism is arranged, so that the fire ring can be conveniently poured out. Therefore, the bottle opening of the culture bottle filled with microorganisms can be slowly rotated to the fire ring, meanwhile, the bottle plug is opened, efficient inoculation in a sterile environment is achieved, meanwhile, through high-precision cooperation of a plurality of mechanisms, the phenomena that the bottle plug is opened too slowly or the dumping angle is improper and the like are avoided, and the inoculation efficiency is improved. The effect of efficiently culturing the microorganisms is further realized.

The application relates to the technical field of medical auxiliary devices, in particular to a blood bag warming device for a blood transfusion department, which comprises a sterilization box, a box cover, an electric push rod one, a heating assembly, a disturbance assembly and a pump suction assembly. The electric push rod one is arranged in the sterilization box and connected with the output end of the box cover. The bottom of the box cover is provided with two mounting racks. The bottom of each of the two mounting racks is provided with an electric push rod two. The heating assembly comprises two clamping plates, and the two clamping plates are arranged on the corresponding mounting racks. The disturbance assembly is provided with two and connected with the output end of the corresponding electric push rod two. The heating assembly, the disturbance assembly and the pump suction assembly can realize synchronous dynamic warming of the two sides of the blood bag in a sterile environment, improve the warming efficiency and avoid the breeding of bacteria to affect the treatment effect.

The invention relates to the technical field of cell separation, and particularly discloses a cell separation device which comprises a main body, the rotating platform is arranged in the main body, and the rotating platform rotates at a high speed to generate centrifugal force, so that target cells are separated from other components; the number of the bases is four, and the four bases are distributed in an annular array mode relative to the center axis of the rotating platform; the first reset spring provides upward elastic force for the sliding block, the second reset spring provides downward elastic force for the clamping seat, and when the centrifugal tube moves along with the clamping seat, the first reset spring simultaneously absorbs power of the clamping seat, protects the centrifugal tube, gently and uniformly mixes, prevents cells from settling to block a filter membrane, but avoids generation of foam; four centrifugal tubes are uniformly mixed in the rotating platform at the same time, so that the experiment process is reduced, a sample is uniformly mixed in a sterile environment, the possibility that the sample is polluted by microorganisms outside the sterile environment is reduced, and the accuracy and the treatment effect of a subsequent experiment are influenced.

The application discloses an indoor seedling raising method of Tricholoma mongolicum, and relates to the technical field of indoor seedling raising, which comprises the following steps: preparing a low-nutrient symbiotic germination culture medium, taking water and agar as the basis, containing 0-5 g / L sugar substances and humus soil extract, and forming a sterile environment beneficial to the symbiosis of fungi; selecting associated fungi to prepare mycelium fragment suspension as symbiotic inoculants through liquid fermentation; performing surface disinfection and sterile ultrasonic treatment on Tricholoma mongolicum seeds; sowing the seeds on the surface of the culture medium and inoculating the symbiotic inoculants under sterile conditions, and culturing under artificial climate control of temperature and light, so as to promote the symbiotic relationship between the fungi and the seeds; and obtaining symbiotic seedlings after the seeds swell and differentiate into protocorms or young leaves. The symbiotic germination mechanism of associated fungi is introduced into the indoor sterile culture system, the emergence rate can reach more than 90%, the emergence uniformity is high, the seedlings are not limited by seasons and climate, the seedlings are self-brought mycorrhizal roots, the transplanting survival rate is high, and the reliable seedling guarantee is provided for the large-scale artificial planting of Tricholoma mongolicum.

A device for providing a sterile environment for the incubation of cell cultures is proposed, comprising a warming chamber with heating elements, sensors for temperature, humidity and CO2, and a gas supply providing a gas connection in the interior. The device also has a cultivation tank located in the warming chamber. This has a sealable passage for each sensor, and also a sealable gas inlet and outlet that couples to the gas connection inside the warming chamber. The sensors of the warming chamber are arranged to fit into respective passages of the cultivation vessel. The device also has a water reservoir arranged in the cultivation tank. The three elements of the warming chamber, the cultivation container and the water reservoir can be sterilized separately using the optimal method for each. Since the sensors are not part of the cultivation container, the latter can also be decontaminated by high temperatures. The sterilized warming chamber ensures that the cell cultures in the cultivation container cannot be contaminated from the outside, nor can they cause contamination in the laboratory.

The utility model discloses a sterilization device for a feed microorganism bacterial liquid delivery pipeline, which comprises a plurality of seed tanks and a plurality of inoculation tanks, the seed tanks and the plurality of inoculation tanks are communicated through liquid delivery pipes and branch pipes, the branch pipes are communicated with a medium outlet end of the sterilization device, and the sterilization device comprises a drying mechanism, a steam mechanism, a main pipe and an auxiliary pipe. The medium outlet end of the drying mechanism is communicated with the main pipe through the auxiliary pipe, the medium outlet end of the steam mechanism is communicated with the main pipe, the medium outlet end of the main pipe is communicated with the branch pipe, and the branch pipe, the main pipe and the auxiliary pipe are all provided with electromagnetic valves. According to the device, two different media can be sequentially introduced into the infusion tube according to the sequence, high-temperature steam is firstly introduced for sterilization, then dry air with temperature is introduced for heating residual water vapor so as to ensure dryness in the infusion tube, the infusion tube is treated by using the device, the sterile environment of the infusion tube is ensured, and the safety of the infusion tube is ensured. And the concentration of the strain is not influenced.

The utility model discloses a 50L bacitracin zinc fermentation tank inoculation tool, and belongs to the technical field of biological strain inoculation. Comprising an inoculating loop, an adjusting base and a windproof assembly, the adjusting base is rotationally installed at the bottom of the inoculating loop, an alcohol cotton groove with an opening in the top is formed between the adjusting base and the inoculating loop, the windproof assembly is vertically and slidably arranged in the alcohol cotton groove in a covering mode, abutting rods are symmetrically installed at the top of the adjusting base, and the abutting rods are matched with the windproof assembly in an abutting mode. The inner wall of the inoculating loop is fixedly connected with an arc-shaped track for the abutting rod to move, and an outlet is formed in one end of the arc-shaped track. By means of alcohol cotton burning in the alcohol cotton groove, the pollution risk in the inoculation process is effectively reduced, the sterile environment in the fermentation process is guaranteed, the inoculation operation is more flexible due to the design of the adjusting base and the arc-shaped track, the windproof assembly can be rapidly lifted by screwing the adjusting base, and the stability of flame burning is enhanced.

This invention provides a sugarcane tissue culture seedling cultivation system, belonging to the field of sugarcane cultivation technology. It includes a sterile environment cultivation chamber, an environmental factor synergistic regulation system, a machine vision-based intelligent management system for culture media and cultures, culture bottles, and culture racks. The culture bottles are placed on the culture racks, which are located within the sterile environment cultivation chamber. This invention establishes a light-temperature-humidity-air linkage model to simulate the optimal growth microenvironment, significantly promoting the robust growth of sugarcane tissue culture seedlings, shortening the cultivation cycle, and increasing the proliferation coefficient and rooting rate. It utilizes machine vision technology to automatically monitor growth parameters such as seedling height, bud number, and root system, accurately determining the optimal time for transfer and termination of rooting culture, eliminating weak, deformed, and contaminated seedlings, and greatly improving the uniformity and quality of seedlings. By optimizing the design of the lighting system and the standardized placement of culture bottles, it ensures uniform light distribution and reduces energy consumption; through precise control, it reduces resource waste.

The invention discloses a boletus aereus solid strain culture method, and relates to the technical field of microbial culture, the method comprises the following steps: preparing a special culture medium taking hard broadleaf sawdust with specific particle size and corncob as main materials, wheat bran and soybean meal as auxiliary materials, and mulberry twig fermentation dry powder as a biological activity induction component; putting the special solid culture medium into a culture bottle, sealing, sterilizing, and cooling for later use; inoculating the boletus aereus mother strain into the sterilized special solid culture medium; a micro-positive pressure intermittent ventilation culture process which is closed in the whole process and does not need punching is adopted for the culture bottle after culture and inoculation. The invention aims to solve the problems of high pollution risk, slow hypha growth and high planting failure rate caused by manual punching due to compact structure and poor air permeability of the existing solid culture gene, realizes the cooperation of high-porosity oxygen supply, precise nutrition supply and sterile environment guarantee, and remarkably improves the hypha activity and culture success rate. The method is suitable for efficient industrial production of boletus aereus solid strains.

The invention discloses a tissue culture method for botrychium ternatum spore induced prothallium. The culture method comprises the following steps: collecting botrychium ternatum with sporangium, drying, sieving and collecting spores, sequentially carrying out multiple times of disinfection and cleaning pretreatment on the collected spores by using 75% ethanol, sterile water, 2% sodium hypochlorite and the like in a sterile environment, uniformly coating a culture medium with treated spore suspension, and carrying out observation culture, the time from spore inoculation to prothallium formation is 24-26 days, and the time from spore inoculation to juvenile sporophyte formation is 47-63 days. The culture medium consists of the following components: a 1 / 8 MS culture medium, 5g / L of sucrose and 8g / L of agar. The culture medium is simple to prepare, can effectively induce spore germination and prothallus proliferation of the botrychium ternatum, can be further cultured to obtain sporophytes, can also be used for acclimatization and transplantation, shortens the seedling raising period, further realizes large-scale cultivation of the botrychium ternatum, and has important significance for expanded application of pteridophyte.

The utility model discloses an auxiliary device for taking liquid from a large-capacity Schottky bottle, and aims to solve the problems of high operation difficulty and poor stability in a liquid sampling process of a Schottky bottle with a pipette in a sterile environment. The device comprises a base, a supporting rod, an arc-shaped hanging wall and a swinging assembly, and the swinging assembly is composed of a bottom support, a clamping plate, a swinging rod and an annular support and can be connected with a bearing at the top of the supporting rod through a rotating shaft to achieve free rotation. A plurality of hook grooves are formed in the arc-shaped hanging wall and can be matched with a clamping rod to fix the swinging assembly at different inclination angles, so that an operator can conveniently sample liquid. And the bottom support is provided with a groove matched with the bottom of the Schottky bottle in shape, so that the stability of the bottle body is enhanced. The whole liquid sampler is made of stainless steel, meets the requirement of a sterile environment, and is suitable for liquid sampling operation in biological product production. The device has the advantages of multi-angle stable support, simplicity and convenience in operation, high safety and low cost, and the sampling efficiency can be remarkably improved.

The invention discloses probiotic salad dressing with an intestinal regulation function and a preparation method of the probiotic salad dressing, and belongs to the technical field of food processing. The salad dressing comprises the following components in parts by weight: 40-60 parts of a grease base material, 5-15 parts of an acidulant, 3-10 parts of probiotic microcapsules, 3-10 parts of seasonings and 10-30 parts of water. The preparation method adopts a core sterile post-addition process, namely, firstly, the salad dressing base is subjected to high-temperature instantaneous sterilization and cooling, and then the probiotics and the sterile base are mixed and filled in a sterile environment. Through combination of a microcapsule embedding technology and a sterile post-addition technology, the problem that probiotics are prone to inactivation in the salad dressing acid environment, the production process and the storage period is effectively solved, it is ensured that the product keeps a high viable count in the whole shelf life, the product can tolerate the human digestive tract environment, and the exact intestinal tract regulation function is achieved.

The invention relates to the technical field of cordyceps militaris culture, in particular to a culture method for planting cordyceps militaris through silkworms, which comprises the following steps: activating a strain through a solid culture medium, transferring into a liquid culture medium, and carrying out shake culture to obtain a hypha suspension; inoculating the bacterial liquid to a silkworm chrysalis substrate subjected to composite coating pretreatment in a sterile environment, and carrying out dark culture under a first condition of low temperature and light shielding until hyphae overgrow; and then converting to a second condition with day and night temperature difference and scattered light to induce primordium formation, and then adjusting to a third condition with constant temperature and high humidity to promote fruiting bodies to be mature and then harvesting. According to the cultivation technology, pollution can be effectively reduced, growth can be promoted, the yield can be effectively increased, the quality of cordyceps militaris sporocarp can be remarkably improved, and the content of main active ingredients, namely cordycepin and polysaccharide, of cordyceps militaris can reach the high level.

The invention relates to a sterile rapid propagation system for gastrodia elata seed breeding, and belongs to the technical field of plant cultivation and breeding. The system mainly comprises a central control unit, a sterile culture cavity, an environment control system, a nutrient solution and strain supply system, a pollution real-time monitoring and intervention system, an automatic transplanting and harvesting unit and a data acquisition and intelligent analysis module. According to the system, by integrating a sterile environment, precise control and intelligent analysis, microbial pollution is completely eradicated, the survival rate of gastrodia elata seeds is remarkably increased, the growth cycle is shortened, and rapid, efficient and large-scale standardized breeding is achieved.

The invention discloses an agrobacterium tumefaciens-mediated peanut instantaneous transformation method, and belongs to the technical field of peanut gene engineering. The invention provides a set of instantaneous genetic transformation technical system of agrobacterium-mediated peanut living plants in a non-sterile environment, which mainly comprises the following steps: cleaning roots of peanut seedlings which are normally hydroponically cultured for 14 days, cutting off lateral root tip parts, injecting agrobacterium to a first stem node and a second stem node of a peanut by using an injector, the method comprises the following steps: taking out seedlings, putting the seedlings in an agrobacterium solution capable of submerging roots, carrying out vacuum negative pressure treatment, taking out the seedlings, carrying out dark culture for 24 hours, carrying out normal culture for 14 days, and then detecting leaves which are instantaneously and successfully converted, so that the method is short in conversion period, independent of a sterile environment, simple and convenient to operate, high in conversion efficiency and capable of being operated on a large scale. According to the instant transformation method of the peanut living plant, transgenic leaves and buds can be rapidly obtained, and a foundation is laid for further gene function research.

An integrated, automated system for quantitative and / or qualitative microbiological and / or analyte evaluation of samples using in situ production culture devices is provided. The system includes: an integrated automation system including sterile media and / or buffer reagents, culture device parts, and automated components for media and / or buffer handling, test sample handling, culture device assembly, and microbial enumeration and / or detection, all within a sterile environment; one or more processors; and memory containing computer-executable instructions that, when executed by the one or more processors, cause the integrated automation system to determine that coded test samples loaded into the system should be quantitatively and / or qualitatively evaluated; and, in response, the system processes and microbiologically evaluates the coded test samples using one or more designated in situ production culture devices, for quantitative evaluation, thereby eliminating the need to sterilize, transport, store, and / or open ex situ-manufactured culture devices prior to inoculation thereof.

The invention relates to the technical field of plant breeding, in particular to an agrobacterium rhizogenes-mediated grape plant genetic transformation method under a non-sterile environment and application of the agrobacterium rhizogenes-mediated grape plant genetic transformation method. The genetic transformation method comprises the following steps: infecting grape seeds with agrobacterium rhizogenes carrying a target gene under the vacuum condition of 0.6-1 kg / cm < 2 > for more than 20 minutes, and ensuring that the OD600 value of the agrobacterium rhizogenes is 0.4-1.2; the grape seeds are grape immature zygotic embryos in a germination state; and the agrobacterium rhizogenes are K599 agrobacterium rhizogenes. The invention provides a genetic transformation method based on a specific seed growth period and agrobacterium tumefaciens, which can obviously improve the transformation efficiency and shorten the period from seed germination to seedling, and has the characteristics of high repeatability and wide applicability. And an efficient and stable genetic transformation technical basis is provided for gene function research and variety improvement of grapes and other grape plants.

The present invention ensures a sterile state in a clean room by eliminating the risk of propagation of bacteria due to frost formation and dew condensation.　A holding window 12a into which a freezing device 11 is fitted is formed penetrating through a wall section 12 that configures a clean room 13 and has a back space 15 on a rear surface side thereof, and a casing 11b of the freezing device 11 is fitted into and held by the holding window 12a. At this time, an opening / closing door 11a of the freezing device 11 is exposed at a surface on the clean room 13 side of the wall section 12. A refrigerant supply passage 14 for supplying a refrigerant to the freezing device 11 is provided in the back space 15 together with a refrigerant container 16, and the portion where frost and dew condensation are likely to occur is thereby isolated.

The application discloses a kind of based on hairy root agrobacterium and RUBY reporter gene-mediated visible genetic transformation method of Radix Ternatae, for the technical obstacle that Radix Ternatae lignification degree is high, agrobacterium is difficult to infect, the application uses the stem base of Radix Ternatae as explant, using the hairy root agrobacterium K599 carrying RUBY reporter gene is infected, through vacuum filtration combination darkness soaking, directly transplanting to nutrient soil after being infected explant, without relying on tissue culture and sterile environment.The application realizes the rapid induction and visual screening of Radix Ternatae transgenic hairy root, and the average 21 days appears and presents characteristic red, and positive induction rate can reach 20%.The application also discloses the function of new gene ThFLS116 of Radix Ternatae for the first time, which regulates root development and flavonoid synthesis metabolism.The application provides an efficient technical platform for gene function analysis and active ingredient industrialization development of Radix Ternatae.

The invention relates to the technical field of food processing, and discloses a duck accessory vacuum production process based on a sterile environment, which comprises the following steps: raw material pretreatment, fresh-keeping steeping liquor preparation, reduced pressure steeping treatment, pulse vacuum packaging, secondary sterilization treatment and sterile cooling and inspection. According to the invention, through the synergistic effect of the components in the composite biological fresh-keeping agent, the natural antibacterial peptide can inhibit the reproduction of microorganisms, the organic acid chelating agent can stabilize the color and luster of the product and inhibit enzymatic browning, and the antioxidant can delay lipid oxidation, so that an efficient fresh-keeping system is jointly constructed; meanwhile, in the programmed controlled pressure-reducing impregnation process, the gradient pressure difference generated by multi-stage vacuum circulation is utilized to promote the fresh-keeping components to deeply permeate into the duck accessory tissues, so that comprehensive fresh keeping from the outside to the inside is realized, the shelf life of the product is prolonged, and the original flavor of the product is kept.

The invention relates to the technical field of plant tissue culture, and discloses an azalea tissue culture and rapid propagation method which comprises the following steps: S1, preparing an azalea explant and a culture medium; s2, preparing a dynamic purification culture device and sterilizing; s3, inoculation and dynamic purification culture: inoculating the stem segments into a proliferation culture medium in a growth area in a sterile environment; starting dynamic purification; s4, carrying out subculture multiplication; s5, rooting and seedling hardening. By arranging the dynamic purification culture device, small molecule metabolites generated in a growth area can be continuously removed in the rhododendron tissue culture process, dependence on traditional antioxidants is not needed, the competitive effect of the antioxidants and metabolite adsorption materials is avoided, the stable culture environment can be maintained for a long time, inhibition of metabolite accumulation on plant cell metabolism is reduced, and the survival rate of the rhododendron is increased. The survival of explants and the normal differentiation and growth of bud clusters are effectively guaranteed, and the stability of the tissue culture process and the overall quality of seedlings are improved.