36 results about "Sterile environment" patented technology

The utility model relates to the technical field of fungus stick inoculation machine provides a fungus stick inoculation machine, including frame, inoculation device, blanking device, eye -boring device, stop device and spray brush sterilization device, and set up in the air purifier and distribution box of frame top, fungus stick removes and sends device and is arranged in the frame inside, the inoculation device includes setting on fungus stick removes and sends device inoculation stick and the blanking device of located inoculation stick top, and inoculation stick removes and send in proper order through spray brush sterilization device, eye -boring device and inoculation device. Through the spray brush sterilization device, eye -boring device, inoculation device and fungus stick removes and send device are integrated in same frame, realize fungus stick disinfection, drilling, inoculation's full process automation continuous operation. Spray brush sterilization device in real time disinfects surface before fungus stick enters drilling station, and the clean air is continuously conveyed to the top operating area in combination with air purifier, and the sterile environment is double -guaranteed.

The invention discloses a microalgae culture device capable of realizing self-adaptive supply of dissolved oxygen by using a waterproof breathable membrane tube, a large number of micropores with pore diameters smaller than those of microalgae cells and bacteria are distributed on the tube wall of the waterproof breathable membrane tube, and the waterproof breathable membrane tube can effectively prevent bacteria in air from entering a fermentation tank, ensure a sterile environment in the tank and improve the utilization rate of the microalgae. Microalgae cells can be prevented from entering the membrane tube to cause cell loss; meanwhile, the wall surface of the waterproof and breathable membrane tube has hydrophobicity, a liquid culture medium can be effectively prevented from entering the membrane tube, a gas supply channel in the membrane tube is prevented from being blocked, and the tube wall of the waterproof and breathable membrane tube only allows gas molecules such as oxygen and carbon dioxide to freely permeate; after the air pump pressurizes ambient air, the ambient air is injected into the waterproof breathable membrane tube from the air inlet of the fermentation tank, and oxygen in the gas in the waterproof breathable membrane tube penetrates through the tube wall under the driving of concentration gradient to enter the suspension type microalgae culture system so as to provide oxygen for heterotrophic growth of microalgae cells.

The application belongs to the technical field of tissue culture and relates to a kind of induction method and application of monkey ear ring flower filament callus, comprising the following steps: taking monkey ear ring flower bud, after being treated with disinfecting liquid for disinfection and sterilization, in sterile environment, take monkey ear ring flower filament as explant, it is inoculated in induction medium and dark culture, form callus, then the callus is transferred to proliferation medium and is subcultured and proliferated, and more high-quality callus is obtained.The induction method provided by the application can induce monkey ear ring flower filament to form callus quickly, and the callus induction rate is as high as 100%;The induction method of monkey ear ring flower filament callus in the application has short cultivation period, high induction efficiency and low contamination rate, the induction method is applied to the cultivation of monkey ear ring, the research path of monkey ear ring rapid propagation technology is widened, the regeneration of monkey ear ring flower filament into plant becomes possible, and high-quality plant with excellent traits can be cultivated.

The utility model relates to the technical field of fermentation tanks, and discloses a fermentation tank which comprises a tank body, a steam conveying pipe is connected to the top of the tank body, an air filter is connected to one end of the steam conveying pipe, a liquid outlet is formed in the bent position of the bottom of the steam conveying pipe, and a first connecting pipe is connected to the liquid outlet of the bottom of the steam conveying pipe. One end of the first connecting pipe is connected with a second connecting pipe through a connecting assembly, and one end of the second connecting pipe is connected with an exhaust valve. According to the fermentation tank, condensate water formed in a steam conveying pipe can flow into a first connecting pipe from a liquid discharging opening in the bent position of the bottom of the steam conveying pipe, and then is discharged from an exhaust valve in cooperation with a second connecting pipe, so that the condensate water deposited at the bent position of the bottom of the steam conveying pipe can be discharged; a large number of bacteria are prevented from entering the fermentation tank along with the steam pipeline due to deposition of condensed water, and the effect of a dry and sterile environment at the bent part of the bottom of the steam pipeline can be ensured.

The invention relates to a plant cultivation method, in particular to a sterile miniature cuttage rapid propagation method for brassica napobrassica. According to the method, stem segments with buds or terminal buds of the brassica napobrassica are used as explants for asexual propagation, budlet micro cutting is performed in a sterile environment, plantlets with roots are directly induced without adding any plant hormones and inducing cluster buds and calluses, and rapid propagation of the brassica napobrassica is realized through continuous micro cutting. The method is not limited by seasons and propagation materials, seedlings can be produced in a manual control environment all the year round, the propagation speed is high, and the coefficient is large.

The present application relates to the technical field of bacterial storage, and particularly relates to a bacterial drug resistance detection storage device. The present application provides a bacterial drug resistance detection storage device which can effectively reduce bacterial infection and more quickly drip wax on a bacterial culture dish. The bacterial drug resistance detection storage device comprises a storage cabinet, a cabinet door, a placing table and a reciprocating moving mechanism, etc. The storage cabinet is divided into a main storage chamber and a sub-storage chamber. The cabinet door is connected to one side of the storage cabinet near the sub-storage chamber through a hinge. The placing table is placed on the bottom of one side of the main storage chamber in the storage cabinet. The reciprocating moving mechanism is arranged on one side of the main storage chamber in the storage cabinet. The motor drives the lead screw to rotate, and then drives the disc to rotate, opens the intermittent liquid feeding pipe, and the alcohol in the intermittent liquid feeding pipe flows to the four rotating liquid spraying pipes, and is uniformly sprayed out through the plurality of spray holes on the rotating liquid spraying pipes, so as to sterilize and disinfect the sub-storage chamber and the placing table of the storage cabinet, and more ensure the sterile environment when dripping wax on the bacterial culture dish.

The invention relates to a method for increasing the sporulation of bacteria such as Bacillus velezensis and Paenibacillus polymyxa using a liquid medium comprising glucose, ammonium sulfate, magnesium chloride and non-ionic surfactant polymer compounds. This method for increasing sporulation is used in a method for generating a large quantity of spores and selecting spores more resistant to higher temperatures than the spores of wild strains. The spores with higher heat resistance than those of the wild strains can be used in a method for eliminating contamination in fermentations.

The invention relates to the technical field of efficient culture of microorganisms, in particular to an efficient culture device of microorganisms, which comprises a culture tank body, a pouring-in pipe arranged on the culture tank body and a sealing cover arranged on the pouring-in pipe, and a guide-in shell is arranged on the outer side of the pouring-in pipe. The guiding-in shell is arranged on the sealing cover, the putting-in opening where the fire ring can be conveniently placed is formed in the guiding-in shell, then the fire ring can be placed at the guiding-in shell, the uncovering mechanism is arranged on the guiding-in shell, then the effect of opening the sealing cover is achieved, and when the sealing cover is opened, the microorganism pouring mechanism is arranged, so that the fire ring can be conveniently poured out. Therefore, the bottle opening of the culture bottle filled with microorganisms can be slowly rotated to the fire ring, meanwhile, the bottle plug is opened, efficient inoculation in a sterile environment is achieved, meanwhile, through high-precision cooperation of a plurality of mechanisms, the phenomena that the bottle plug is opened too slowly or the dumping angle is improper and the like are avoided, and the inoculation efficiency is improved. The effect of efficiently culturing the microorganisms is further realized.

The application relates to the technical field of medical auxiliary devices, in particular to a blood bag warming device for a blood transfusion department, which comprises a sterilization box, a box cover, an electric push rod one, a heating assembly, a disturbance assembly and a pump suction assembly. The electric push rod one is arranged in the sterilization box and connected with the output end of the box cover. The bottom of the box cover is provided with two mounting racks. The bottom of each of the two mounting racks is provided with an electric push rod two. The heating assembly comprises two clamping plates, and the two clamping plates are arranged on the corresponding mounting racks. The disturbance assembly is provided with two and connected with the output end of the corresponding electric push rod two. The heating assembly, the disturbance assembly and the pump suction assembly can realize synchronous dynamic warming of the two sides of the blood bag in a sterile environment, improve the warming efficiency and avoid the breeding of bacteria to affect the treatment effect.

The invention relates to the technical field of cell separation, and particularly discloses a cell separation device which comprises a main body, the rotating platform is arranged in the main body, and the rotating platform rotates at a high speed to generate centrifugal force, so that target cells are separated from other components; the number of the bases is four, and the four bases are distributed in an annular array mode relative to the center axis of the rotating platform; the first reset spring provides upward elastic force for the sliding block, the second reset spring provides downward elastic force for the clamping seat, and when the centrifugal tube moves along with the clamping seat, the first reset spring simultaneously absorbs power of the clamping seat, protects the centrifugal tube, gently and uniformly mixes, prevents cells from settling to block a filter membrane, but avoids generation of foam; four centrifugal tubes are uniformly mixed in the rotating platform at the same time, so that the experiment process is reduced, a sample is uniformly mixed in a sterile environment, the possibility that the sample is polluted by microorganisms outside the sterile environment is reduced, and the accuracy and the treatment effect of a subsequent experiment are influenced.

A device for providing a sterile environment for the incubation of cell cultures is proposed, comprising a warming chamber with heating elements, sensors for temperature, humidity and CO2, and a gas supply providing a gas connection in the interior. The device also has a cultivation tank located in the warming chamber. This has a sealable passage for each sensor, and also a sealable gas inlet and outlet that couples to the gas connection inside the warming chamber. The sensors of the warming chamber are arranged to fit into respective passages of the cultivation vessel. The device also has a water reservoir arranged in the cultivation tank. The three elements of the warming chamber, the cultivation container and the water reservoir can be sterilized separately using the optimal method for each. Since the sensors are not part of the cultivation container, the latter can also be decontaminated by high temperatures. The sterilized warming chamber ensures that the cell cultures in the cultivation container cannot be contaminated from the outside, nor can they cause contamination in the laboratory.

The utility model discloses a sterilization device for a feed microorganism bacterial liquid delivery pipeline, which comprises a plurality of seed tanks and a plurality of inoculation tanks, the seed tanks and the plurality of inoculation tanks are communicated through liquid delivery pipes and branch pipes, the branch pipes are communicated with a medium outlet end of the sterilization device, and the sterilization device comprises a drying mechanism, a steam mechanism, a main pipe and an auxiliary pipe. The medium outlet end of the drying mechanism is communicated with the main pipe through the auxiliary pipe, the medium outlet end of the steam mechanism is communicated with the main pipe, the medium outlet end of the main pipe is communicated with the branch pipe, and the branch pipe, the main pipe and the auxiliary pipe are all provided with electromagnetic valves. According to the device, two different media can be sequentially introduced into the infusion tube according to the sequence, high-temperature steam is firstly introduced for sterilization, then dry air with temperature is introduced for heating residual water vapor so as to ensure dryness in the infusion tube, the infusion tube is treated by using the device, the sterile environment of the infusion tube is ensured, and the safety of the infusion tube is ensured. And the concentration of the strain is not influenced.

The utility model discloses a 50L bacitracin zinc fermentation tank inoculation tool, and belongs to the technical field of biological strain inoculation. Comprising an inoculating loop, an adjusting base and a windproof assembly, the adjusting base is rotationally installed at the bottom of the inoculating loop, an alcohol cotton groove with an opening in the top is formed between the adjusting base and the inoculating loop, the windproof assembly is vertically and slidably arranged in the alcohol cotton groove in a covering mode, abutting rods are symmetrically installed at the top of the adjusting base, and the abutting rods are matched with the windproof assembly in an abutting mode. The inner wall of the inoculating loop is fixedly connected with an arc-shaped track for the abutting rod to move, and an outlet is formed in one end of the arc-shaped track. By means of alcohol cotton burning in the alcohol cotton groove, the pollution risk in the inoculation process is effectively reduced, the sterile environment in the fermentation process is guaranteed, the inoculation operation is more flexible due to the design of the adjusting base and the arc-shaped track, the windproof assembly can be rapidly lifted by screwing the adjusting base, and the stability of flame burning is enhanced.

This invention provides a sugarcane tissue culture seedling cultivation system, belonging to the field of sugarcane cultivation technology. It includes a sterile environment cultivation chamber, an environmental factor synergistic regulation system, a machine vision-based intelligent management system for culture media and cultures, culture bottles, and culture racks. The culture bottles are placed on the culture racks, which are located within the sterile environment cultivation chamber. This invention establishes a light-temperature-humidity-air linkage model to simulate the optimal growth microenvironment, significantly promoting the robust growth of sugarcane tissue culture seedlings, shortening the cultivation cycle, and increasing the proliferation coefficient and rooting rate. It utilizes machine vision technology to automatically monitor growth parameters such as seedling height, bud number, and root system, accurately determining the optimal time for transfer and termination of rooting culture, eliminating weak, deformed, and contaminated seedlings, and greatly improving the uniformity and quality of seedlings. By optimizing the design of the lighting system and the standardized placement of culture bottles, it ensures uniform light distribution and reduces energy consumption; through precise control, it reduces resource waste.

The invention discloses a boletus aereus solid strain culture method, and relates to the technical field of microbial culture, the method comprises the following steps: preparing a special culture medium taking hard broadleaf sawdust with specific particle size and corncob as main materials, wheat bran and soybean meal as auxiliary materials, and mulberry twig fermentation dry powder as a biological activity induction component; putting the special solid culture medium into a culture bottle, sealing, sterilizing, and cooling for later use; inoculating the boletus aereus mother strain into the sterilized special solid culture medium; a micro-positive pressure intermittent ventilation culture process which is closed in the whole process and does not need punching is adopted for the culture bottle after culture and inoculation. The invention aims to solve the problems of high pollution risk, slow hypha growth and high planting failure rate caused by manual punching due to compact structure and poor air permeability of the existing solid culture gene, realizes the cooperation of high-porosity oxygen supply, precise nutrition supply and sterile environment guarantee, and remarkably improves the hypha activity and culture success rate. The method is suitable for efficient industrial production of boletus aereus solid strains.

The invention discloses a tissue culture method for botrychium ternatum spore induced prothallium. The culture method comprises the following steps: collecting botrychium ternatum with sporangium, drying, sieving and collecting spores, sequentially carrying out multiple times of disinfection and cleaning pretreatment on the collected spores by using 75% ethanol, sterile water, 2% sodium hypochlorite and the like in a sterile environment, uniformly coating a culture medium with treated spore suspension, and carrying out observation culture, the time from spore inoculation to prothallium formation is 24-26 days, and the time from spore inoculation to juvenile sporophyte formation is 47-63 days. The culture medium consists of the following components: a 1 / 8 MS culture medium, 5g / L of sucrose and 8g / L of agar. The culture medium is simple to prepare, can effectively induce spore germination and prothallus proliferation of the botrychium ternatum, can be further cultured to obtain sporophytes, can also be used for acclimatization and transplantation, shortens the seedling raising period, further realizes large-scale cultivation of the botrychium ternatum, and has important significance for expanded application of pteridophyte.

The invention discloses probiotic salad dressing with an intestinal regulation function and a preparation method of the probiotic salad dressing, and belongs to the technical field of food processing. The salad dressing comprises the following components in parts by weight: 40-60 parts of a grease base material, 5-15 parts of an acidulant, 3-10 parts of probiotic microcapsules, 3-10 parts of seasonings and 10-30 parts of water. The preparation method adopts a core sterile post-addition process, namely, firstly, the salad dressing base is subjected to high-temperature instantaneous sterilization and cooling, and then the probiotics and the sterile base are mixed and filled in a sterile environment. Through combination of a microcapsule embedding technology and a sterile post-addition technology, the problem that probiotics are prone to inactivation in the salad dressing acid environment, the production process and the storage period is effectively solved, it is ensured that the product keeps a high viable count in the whole shelf life, the product can tolerate the human digestive tract environment, and the exact intestinal tract regulation function is achieved.

The invention relates to the technical field of cordyceps militaris culture, in particular to a culture method for planting cordyceps militaris through silkworms, which comprises the following steps: activating a strain through a solid culture medium, transferring into a liquid culture medium, and carrying out shake culture to obtain a hypha suspension; inoculating the bacterial liquid to a silkworm chrysalis substrate subjected to composite coating pretreatment in a sterile environment, and carrying out dark culture under a first condition of low temperature and light shielding until hyphae overgrow; and then converting to a second condition with day and night temperature difference and scattered light to induce primordium formation, and then adjusting to a third condition with constant temperature and high humidity to promote fruiting bodies to be mature and then harvesting. According to the cultivation technology, pollution can be effectively reduced, growth can be promoted, the yield can be effectively increased, the quality of cordyceps militaris sporocarp can be remarkably improved, and the content of main active ingredients, namely cordycepin and polysaccharide, of cordyceps militaris can reach the high level.

The invention relates to a sterile rapid propagation system for gastrodia elata seed breeding, and belongs to the technical field of plant cultivation and breeding. The system mainly comprises a central control unit, a sterile culture cavity, an environment control system, a nutrient solution and strain supply system, a pollution real-time monitoring and intervention system, an automatic transplanting and harvesting unit and a data acquisition and intelligent analysis module. According to the system, by integrating a sterile environment, precise control and intelligent analysis, microbial pollution is completely eradicated, the survival rate of gastrodia elata seeds is remarkably increased, the growth cycle is shortened, and rapid, efficient and large-scale standardized breeding is achieved.

An integrated, automated system for quantitative and / or qualitative microbiological and / or analyte evaluation of samples using in situ production culture devices is provided. The system includes: an integrated automation system including sterile media and / or buffer reagents, culture device parts, and automated components for media and / or buffer handling, test sample handling, culture device assembly, and microbial enumeration and / or detection, all within a sterile environment; one or more processors; and memory containing computer-executable instructions that, when executed by the one or more processors, cause the integrated automation system to determine that coded test samples loaded into the system should be quantitatively and / or qualitatively evaluated; and, in response, the system processes and microbiologically evaluates the coded test samples using one or more designated in situ production culture devices, for quantitative evaluation, thereby eliminating the need to sterilize, transport, store, and / or open ex situ-manufactured culture devices prior to inoculation thereof.

The invention relates to the technical field of plant breeding, in particular to an agrobacterium rhizogenes-mediated grape plant genetic transformation method under a non-sterile environment and application of the agrobacterium rhizogenes-mediated grape plant genetic transformation method. The genetic transformation method comprises the following steps: infecting grape seeds with agrobacterium rhizogenes carrying a target gene under the vacuum condition of 0.6-1 kg / cm < 2 > for more than 20 minutes, and ensuring that the OD600 value of the agrobacterium rhizogenes is 0.4-1.2; the grape seeds are grape immature zygotic embryos in a germination state; and the agrobacterium rhizogenes are K599 agrobacterium rhizogenes. The invention provides a genetic transformation method based on a specific seed growth period and agrobacterium tumefaciens, which can obviously improve the transformation efficiency and shorten the period from seed germination to seedling, and has the characteristics of high repeatability and wide applicability. And an efficient and stable genetic transformation technical basis is provided for gene function research and variety improvement of grapes and other grape plants.

The present invention ensures a sterile state in a clean room by eliminating the risk of propagation of bacteria due to frost formation and dew condensation.　A holding window 12a into which a freezing device 11 is fitted is formed penetrating through a wall section 12 that configures a clean room 13 and has a back space 15 on a rear surface side thereof, and a casing 11b of the freezing device 11 is fitted into and held by the holding window 12a. At this time, an opening / closing door 11a of the freezing device 11 is exposed at a surface on the clean room 13 side of the wall section 12. A refrigerant supply passage 14 for supplying a refrigerant to the freezing device 11 is provided in the back space 15 together with a refrigerant container 16, and the portion where frost and dew condensation are likely to occur is thereby isolated.

The present application relates to the technical field of mineral processing, and particularly relates to a desulfurization smelting process for high-sulfur tin concentrate, comprising the following steps: ore pretreatment, tin ore is crushed, ground and screened to obtain ore powder; bacteria cultivation and inoculation, bacteria suitable for high-sulfur environment are selected and inoculated into a small-scale cultivation tank and cultivated in a sterile environment; leaching reaction, the pretreated ore powder is mixed with water, bacterial culture solution is added to prepare ore slurry, and the ore slurry is leached; solution treatment and tin recovery, tin metal is recovered after solid-liquid separation of the ore slurry, and waste liquid is treated.The present application provides a desulfurization smelting process for high-sulfur tin concentrate, effectively solves the problems of environmental pollution and high cost existing in the traditional smelting process through the bacterial leaching method, bacteria can grow and reproduce quickly in a high-sulfur environment, so that the desulfurization efficiency is improved, the process operation is simple, low in cost and friendly to the environment, and the tin recovery rate can be significantly improved.

An integrated, automated system for quantitative and / or qualitative microbiological and / or analyte evaluation of samples using in situ production and culture devices is provided. The system includes an integrated automation system including sterile media and / or buffer reagents, culture device parts, and automated components for media and / or buffer handling, test sample handling, culture device assembly, and microbial enumeration and / or detection, all within a sterile environment; one or more processors; and memory containing computer-executable instructions that, when executed by the one or more processors, cause the integrated automation system to determine that a sample should be quantitatively and / or qualitatively evaluated, and, in response, process and microbiologically evaluate the coded test sample using one or more designated in situ production and culture devices for quantitative evaluation, thereby eliminating the need to sterilize, transport, store, and / or open the ex situ-manufactured culture device prior to inoculation thereof.

The present application relates to a kind of monocotyledon green wisteria rapid propagation method, using the characteristics of green wisteria specific tissue cell totipotency, under open condition, using liquid medium to induce green wisteria specific site of in vitro leaf split differentiation, produce regenerative root and regenerative bud, finally form plant.The present application aims at the rapid propagation method of green wisteria leaf, without sterile environment, convenient to take material, easy to operate, low in cost, short in period, high in survival rate, with the advantages of high efficiency, economy, easy to scale, factory, etc., in green wisteria rapid seedling, specific material preservation and propagation has greater application value.

This invention discloses a method for preparing fish bones for fishbone painting, comprising: cooking the fish to separate the flesh from the bones; peeling the muscle tissue using a sterilized fine peeling tool in a sterile environment; sequentially performing degreasing and bleaching treatments; and washing and drying to obtain fish bones for fishbone painting. This invention avoids bone contamination and structural damage through fine peeling in a sterile environment. Combined with multi-stage degreasing and controlled bleaching processes, it effectively removes muscle, lipids, and odors, resulting in fish bones with high whiteness, intact structure, strong antioxidant properties, and resistance to yellowing and mold. Optionally, they may also have a natural floral fragrance. This invention provides high-quality raw materials for fishbone painting and other bone art pieces, suitable for industrial production.

This utility model belongs to the technical field of microbial fermentation and spore extraction system equipment, specifically relating to a sterile material handling device for a *Chaetomium globosum* solid-state fermentation tank. It includes a material handling rod handle, a silicone corrugated tube, and a storage tube connected in sequence, and a material handling rod that passes through the internal cavities of the silicone corrugated tube and the storage tube. One end of the material handling rod is fixedly connected to the handle, and the other end is equipped with a material handling ring. This utility model achieves sealed material handling through the coordinated action of the material handling rod handle, the silicone corrugated tube, the storage tube, and the material handling rod, ensuring no leakage or contamination during material transfer. The silicone corrugated tube, due to its elasticity, fits tightly against the material handling rod, forming a dynamic seal. The elasticity of the silicone corrugated tube allows the material handling rod to move back and forth while maintaining a seal, providing a sterile environment for the rod's movement.

The utility model relates to composite bacteria culture technical field, concretely relates to a kind of composite bacteria mixed incubator, including box, box side wall is equipped with observation window, the inside surface of observation window is attached with transparent heating film, box outside is equipped with temperature controller, pivot is rotationally connected on the box, the end of pivot penetrates box and is connected with connecting plate, the side wall of connecting plate is equipped with flexible scraper, flexible scraper is attached with transparent heating film, transparent heating film is powered on and heated by temperature controller control, the temperature of the inner surface of observation window is promoted, when humid hot air in the box contacts observation window, it is not easy to condense into mist, by rotating pivot, and then driving connecting plate synchronous rotation, flexible scraper on connecting plate slides on the surface of transparent heating film, scrape possible residual water mist or stain, heating film prevents mist condensation, cooperate the use of flexible scraper to reduce observation window blur problem, avoid to open box and wipe observation window frequently and damage aseptic environment in the box or influence temperature, humidity and other culture parameters.

A kind of laboratory device of long time low oxygen culture cell of flexible stability, assembly structure is simple, each block is flexibly detached in use, low oxygen gas flow rate is stable in culture, environment is sterile environment, humidity is sufficient, realize the stable culture of cell in low oxygen environment for long time.It includes gas cylinder (1), bacteria filter (3), cell low oxygen incubation chamber (5);Cell low oxygen incubation chamber includes water tank (51), metal bucket (52), glass cover (53), sealing rubber ring (54);Water tank is equipped with electric plug (514), predetermined temperature is obtained by electrification heating to water in water tank, water tank has the heat preservation cover with hole;Metal bucket, glass cover and sealing rubber ring form closed structure, the lower part of metal bucket body is immersed in the water of water tank, the upper part of metal bucket body passes through the hole of heat preservation cover and exposes water tank.

The utility model relates to the technical field of direct drinking machines, and discloses a direct drinking machine water tank with a sterile structure, which comprises a tank body, a sealing cover is arranged on the top surface of the tank body, an annular airtight groove is arranged on the outer side surface of the sealing cover, a sealing rubber ring is fixedly arranged in the annular airtight groove, and a transparent water level groove is arranged on the front side surface of the tank body. The contact area between the sealing cover and the box body can be increased through the annular airtight groove and the sealing rubber ring, so that sealing is tighter, external air, dust, microorganisms and the like are prevented from entering the box body, and the sterile environment of equipment is kept; and the bacteriostatic air pressure balance valve can automatically adjust the air pressure in the box body, so that the air pressure and the external atmospheric pressure are kept balanced, the box body can be prevented from being deformed or damaged due to the air pressure difference, air entering the box body can be filtered, external bacteria, dust and other pollutants are prevented from entering the box body, and therefore secondary pollution of water in the box body is avoided.