130 results about "Sterile environment" patented technology

The invention discloses a sludge treatment method and system, and relates to the field of solid waste treatment, and the method specifically comprises the following operation steps: S1, multi-frequency resonance pretreatment: destroying a sludge colloid structure through stepped mechanical vibration: collapsing an extracellular polymer network at a low frequency, breaking down microbial cell walls at an intermediate frequency to release intracellular water, pathogenic bacteria are inactivated through a high-frequency cavitation effect; s2, performing electrochemical in-situ flocculation; s3, gradient pressure dehydration; s4, low-temperature mineralization stabilization; and S5, vacuum condensation and recovery. According to the sludge treatment method and system, through stepped low-frequency, medium-frequency and high-frequency mechanical resonance, the colloid structure of the sludge can be directionally damaged, the fluidity of the sludge is remarkably improved, chemical conditioners are remarkably reduced, agent residues and secondary pollution are avoided, pathogens are physically inactivated through the high-frequency cavitation effect, and the treatment effect of the sludge is improved. Micro-nano oxygen bubbles are synchronously generated to strengthen free radical oxidation, and a sterile environment is created for subsequent mineralization.

The invention provides application of a bacteriostatic agent in open type tissue culture and rapid propagation of polyploidy bamboo reeds, and relates to the technical field of tissue culture and rapid propagation of plants, the bacteriostatic agent is applied to the open type tissue culture and rapid propagation process of the polyploidy bamboo reeds, and the use method is that the bacteriostatic agent is added into a culture medium for use or sprayed or oscillated and flushed for use, and the bacteriostatic agent is applied to the open type tissue culture and rapid propagation process of the polyploidy bamboo reeds. The bacteriostatic agent is formed by mixing isothiazolinone and sodium dehydroacetate, the mass addition ratio of the isothiazolinone to the sodium dehydroacetate is (8-5): (2-5), and the method comprises the following steps: S1, primary culture of explants; s2, multiplication culture of seedlings; s3, rooting culture, wherein each liter of the culture medium contains 0.05-1g of the bacteriostatic agent in the above steps. The method has the beneficial effects that under the condition that the method does not need to be controlled in a sterile environment and does not depend on the quality of an inoculator, a large amount of propagation of the polyploidy bamboo reed tissue culture seedlings is realized by adopting an open tissue culture technical mode, the productivity speed of a tissue culture center is improved, and the construction threshold and cost of the tissue culture center are reduced.

The invention belongs to the field of microbial agents, and particularly relates to a microbial composite agent for degrading herbicides and a preparation method of the microbial composite agent. The preparation method of the microbial complex microbial inoculant comprises the following preparation steps: mixing bran hydrolysate, peat soil and diatomite, sterilizing, adding a protective agent and a complex flora suspension in a sterile environment, uniformly mixing, kneading and forming, preparing into slices, naturally air-drying, and crushing to obtain the microbial inoculant, and uniformly mixing the microbial agent with the adsorbent, the surfactant and the nutrient substances to obtain the microbial complex microbial inoculant. According to the invention, domesticated microorganisms form a composite flora to prepare the microbial agent, and the composite flora exerts unique degradation capability and generates synergistic effect, so that the herbicide can be degraded more comprehensively and efficiently, and the proportion of each microorganism, an adsorbent, a surfactant and other components in the microbial agent can be adjusted according to different herbicide types and polluted environments; and efficient degradation of specific herbicides is realized.

The invention relates to the technical field of biopharmaceutical equipment, in particular to a sterile culture method and a sterile culture system. The sterile culture system comprises a cell preparation device, an incubator and a cell culture device, the cell preparation device comprises a preparation chamber, and a first sealing valve is arranged on the side wall of the preparation chamber; the incubator is provided with a culture chamber, a second sealing valve is arranged on the side wall of the culture chamber, and the second sealing valve is used for being matched with the first sealing valve, so that the incubator and the cell preparation device are in sealed butt joint or sealed separation; the cell culture device is used for accommodating the culture box, supplying power and gas to the culture box and controlling the cell growth environment in the culture box. As the cells always grow in the sterile environment, the pollution risk is low, frequent sterilization is not needed, the normal growth of the cells is prevented from being influenced by frequent sterilization, the culture time is saved, and the cell preparation and culture efficiency is high.

The invention provides a regulation and control method for prolonging the refreshing time of prunes, which comprises the following steps: disinfecting a phase-temperature fresh-keeping storehouse for 24 hours by using absolute ethyl alcohol, and ventilating for 8-10 hours to ensure a sterile environment. During harvesting, prunes which are free of plant diseases and insect pests, free of mechanical damage, complete in fruit cream and larger than or equal to 20% in sugar content are selected, and the temperature of the prunes is rapidly reduced to 2-2.2 DEG C within 10 h through portable differential pressure precooling equipment. After precooling, a methyl jasmonate aqueous solution is adopted for electrostatic atomization spraying, and 1-methylcyclopropene is used for airtight fumigation for 18-20 h to inhibit ethylene generation and respiration. And then, storing in a phase temperature warehouse at the temperature of-1.5 to-1.2 DEG C, controlling the relative humidity to be 85-87%, and carrying out intermittent nano atomization sterilization by using a hydrogen peroxide aqueous solution once every 15-18 days. According to the method, the fresh-keeping effect of the prunes is comprehensively improved, the safety and the quality of the fruits are ensured, and the fresh-keeping period is remarkably prolonged.

The present invention belongs to the field of natural compounds, and specifically relates to a crude extract of frog follicles, the VMO1 protein, and their extraction methods and uses. The specific technical solution is as follows: A crude extract, wherein the crude extract is from frog follicles and does not contain egg grains. The preparation method includes: extracting the follicles of frogs, removing the egg grains under a sterile environment, adding a buffer solution, performing ultrasonic treatment and centrifugation, taking the supernatant, and filtering to obtain the crude extract. The crude extract obtained by the present invention has good maintained activity and can be further developed for subsequent uses. The crude extract obtained by the present invention can significantly reduce the surface tension, and at the same time has good antioxidant activity and good antibacterial ability, such as the ability to inhibit Pseudomonas aeruginosa. Since products derived from natural products have good biocompatibility, therefore, based on the above activities and functions, the crude extract provided by the present invention has good application prospects in multiple fields such as the field of cosmetics water retention, the field of food preservation, and the field of pharmaceuticals.

The utility model relates to the technical field of fungus stick inoculation machine provides a fungus stick inoculation machine, including frame, inoculation device, blanking device, eye -boring device, stop device and spray brush sterilization device, and set up in the air purifier and distribution box of frame top, fungus stick removes and sends device and is arranged in the frame inside, the inoculation device includes setting on fungus stick removes and sends device inoculation stick and the blanking device of located inoculation stick top, and inoculation stick removes and send in proper order through spray brush sterilization device, eye -boring device and inoculation device. Through the spray brush sterilization device, eye -boring device, inoculation device and fungus stick removes and send device are integrated in same frame, realize fungus stick disinfection, drilling, inoculation's full process automation continuous operation. Spray brush sterilization device in real time disinfects surface before fungus stick enters drilling station, and the clean air is continuously conveyed to the top operating area in combination with air purifier, and the sterile environment is double -guaranteed.

The utility model provides a strain fermentation tank inoculation device which comprises a barrel and a flame ring, the barrel can be arranged on an inoculation opening in a sleeving mode, a sleeve is fixed to a fermentation tank, a net ring is fixed to the inner wall of the barrel, the flame ring is annularly fixed to the upper end of the net ring, and a conical cover integrally formed with the barrel is arranged at the upper end of the barrel. The top of the conical cover is provided with a through hole capable of pouring bacterial liquid for inoculation through the conical flask. According to the inoculation device for the strain fermentation tank, when a flame inoculation method is used for strain inoculation, flames generated by igniting the flame ring are limited in the barrel, the effects that the flames cover the inoculation opening to keep a sterile environment and prevent infectious microbes from entering the fermentation tank are achieved, diffusion of surrounding flames is limited through the barrel and the conical cover, and the inoculation effect is improved. And the risk that operators are scalded is reduced.

The invention discloses a microalgae culture device capable of realizing self-adaptive supply of dissolved oxygen by using a waterproof breathable membrane tube, a large number of micropores with pore diameters smaller than those of microalgae cells and bacteria are distributed on the tube wall of the waterproof breathable membrane tube, and the waterproof breathable membrane tube can effectively prevent bacteria in air from entering a fermentation tank, ensure a sterile environment in the tank and improve the utilization rate of the microalgae. Microalgae cells can be prevented from entering the membrane tube to cause cell loss; meanwhile, the wall surface of the waterproof and breathable membrane tube has hydrophobicity, a liquid culture medium can be effectively prevented from entering the membrane tube, a gas supply channel in the membrane tube is prevented from being blocked, and the tube wall of the waterproof and breathable membrane tube only allows gas molecules such as oxygen and carbon dioxide to freely permeate; after the air pump pressurizes ambient air, the ambient air is injected into the waterproof breathable membrane tube from the air inlet of the fermentation tank, and oxygen in the gas in the waterproof breathable membrane tube penetrates through the tube wall under the driving of concentration gradient to enter the suspension type microalgae culture system so as to provide oxygen for heterotrophic growth of microalgae cells.

The invention provides a granular fat low-temperature collecting device for fat transplantation, and relates to the technical field of fat transplantation. The sealing mechanism is fixedly mounted in the storage mechanism; the top of the automatic blocking mechanism is inserted into the storage mechanism and is communicated with the sealing mechanism; the top of the fat granule docking mechanism is connected with the automatic blocking mechanism; the auxiliary cleaning mechanism is mounted at the top of the storage mechanism; during butt joint conveying, injection of excess air can be greatly reduced; the collection and storage barrel is independently arranged in the storage mechanism; butt joint conveying can be matched, and automatic opening, closing and blocking can be carried out; internal cleaning of the multiple sets of collection and storage barrels can be completed rapidly and conveniently; the problems that if air in an internal area of a bearing container contains bacteria or other substances, fat granules can be influenced to a certain extent, and the temperature and the sterile environment in a heat preservation box can be influenced to a certain extent due to frequent opening, closing and taking are solved.

The invention relates to the technical field of food processing, and particularly discloses a processing method for preparing sea cucumber viscera into powder. The raw materials of the product comprise fresh sea cucumber viscera and composite auxiliary materials, wherein the composite auxiliary materials comprise marine fish collagen peptide and euphausia superba powder; the preparation method comprises the following steps: pretreating fresh sea cucumbers, taking out viscera, cleaning, mixing the viscera with composite auxiliary materials, pulping, filtering to obtain composite juice, carrying out ultrasonic-assisted enzymatic degradation on the composite juice, inactivating bio-enzyme, carrying out low-temperature concentration and drying to obtain primary powder, carrying out non-thermal sterilization and high-pressure sterilization on the primary powder, and packaging to obtain the sea cucumber beverage. And cooling and filling in a sterile environment. The product can be used as a functional food raw material, and has the advantages of reservation of heat-sensitive active ingredients such as sea cucumber saponin and stable quality; in addition, the preparation method disclosed by the invention has the advantages of efficiently utilizing byproducts of the internal organs of the sea cucumbers, being mild in process, not destroying active ingredients and controllable in operation, and resource waste and active ingredient loss can be reduced.

The application belongs to the technical field of tissue culture and relates to a kind of induction method and application of monkey ear ring flower filament callus, comprising the following steps: taking monkey ear ring flower bud, after being treated with disinfecting liquid for disinfection and sterilization, in sterile environment, take monkey ear ring flower filament as explant, it is inoculated in induction medium and dark culture, form callus, then the callus is transferred to proliferation medium and is subcultured and proliferated, and more high-quality callus is obtained.The induction method provided by the application can induce monkey ear ring flower filament to form callus quickly, and the callus induction rate is as high as 100%;The induction method of monkey ear ring flower filament callus in the application has short cultivation period, high induction efficiency and low contamination rate, the induction method is applied to the cultivation of monkey ear ring, the research path of monkey ear ring rapid propagation technology is widened, the regeneration of monkey ear ring flower filament into plant becomes possible, and high-quality plant with excellent traits can be cultivated.

The utility model relates to the technical field of fermentation tanks, and discloses a fermentation tank which comprises a tank body, a steam conveying pipe is connected to the top of the tank body, an air filter is connected to one end of the steam conveying pipe, a liquid outlet is formed in the bent position of the bottom of the steam conveying pipe, and a first connecting pipe is connected to the liquid outlet of the bottom of the steam conveying pipe. One end of the first connecting pipe is connected with a second connecting pipe through a connecting assembly, and one end of the second connecting pipe is connected with an exhaust valve. According to the fermentation tank, condensate water formed in a steam conveying pipe can flow into a first connecting pipe from a liquid discharging opening in the bent position of the bottom of the steam conveying pipe, and then is discharged from an exhaust valve in cooperation with a second connecting pipe, so that the condensate water deposited at the bent position of the bottom of the steam conveying pipe can be discharged; a large number of bacteria are prevented from entering the fermentation tank along with the steam pipeline due to deposition of condensed water, and the effect of a dry and sterile environment at the bent part of the bottom of the steam pipeline can be ensured.

The invention relates to a plant cultivation method, in particular to a sterile miniature cuttage rapid propagation method for brassica napobrassica. According to the method, stem segments with buds or terminal buds of the brassica napobrassica are used as explants for asexual propagation, budlet micro cutting is performed in a sterile environment, plantlets with roots are directly induced without adding any plant hormones and inducing cluster buds and calluses, and rapid propagation of the brassica napobrassica is realized through continuous micro cutting. The method is not limited by seasons and propagation materials, seedlings can be produced in a manual control environment all the year round, the propagation speed is high, and the coefficient is large.

The utility model discloses an active medical device. The active medical equipment comprises a body which is provided with a heat dissipation channel, and the heat dissipation channel penetrates through the body in the first direction; a sealing cavity is formed in the body, the sealing cavity surrounds the heat dissipation channel, and the sealing cavity and the heat dissipation channel are isolated from each other; and the functional device is arranged in the sealing cavity. The active medical equipment provided by the embodiment of the utility model can effectively reduce the pollution of dust and bacteria in the bacteria environment to the sterile environment in the process of alternately using in the bacteria environment and the sterile environment.

The present application relates to the technical field of bacterial storage, and particularly relates to a bacterial drug resistance detection storage device. The present application provides a bacterial drug resistance detection storage device which can effectively reduce bacterial infection and more quickly drip wax on a bacterial culture dish. The bacterial drug resistance detection storage device comprises a storage cabinet, a cabinet door, a placing table and a reciprocating moving mechanism, etc. The storage cabinet is divided into a main storage chamber and a sub-storage chamber. The cabinet door is connected to one side of the storage cabinet near the sub-storage chamber through a hinge. The placing table is placed on the bottom of one side of the main storage chamber in the storage cabinet. The reciprocating moving mechanism is arranged on one side of the main storage chamber in the storage cabinet. The motor drives the lead screw to rotate, and then drives the disc to rotate, opens the intermittent liquid feeding pipe, and the alcohol in the intermittent liquid feeding pipe flows to the four rotating liquid spraying pipes, and is uniformly sprayed out through the plurality of spray holes on the rotating liquid spraying pipes, so as to sterilize and disinfect the sub-storage chamber and the placing table of the storage cabinet, and more ensure the sterile environment when dripping wax on the bacterial culture dish.

The invention relates to the technical field of microorganism culture, in particular to a ganoderma sinensis spore germination culture medium and application thereof.Ganoderma sinensis spores are collected through a fungus carrying bag or the culture medium, the optimal growth environment for ganoderma sinensis spore germination culture is obtained by optimizing the culture condition of the ganoderma sinensis spores, and the ganoderma sinensis spore germination culture medium is obtained by optimizing the culture medium formula and adding wood chip boiling liquid. A culture medium formula most suitable for ganoderma spore germination is obtained through raw material screening and orthogonal experiment optimization of boiling liquid, ganoderma spores can be effectively collected without reaching a sterile environment of a laboratory, the method is a practical and effective ganoderma sinensis spore collection and germination method, and a foundation is laid for promoting ganoderma sinensis new variety breeding research.

The utility model relates to the technical field of sterilization equipment, in particular to a water bath sterilization all-in-one machine which comprises a supporting frame and a supporting disc, a conveying belt is arranged at the top of the supporting frame, the supporting disc is arranged at the top of the conveying belt, filling bottles are movably connected into the supporting disc, and connecting frames are fixedly connected to the two sides of the top of the supporting frame and located above the conveying belt. Five heating water outlet nozzles are fixedly connected to one side in the connecting frame at equal intervals, water blowing pipes are fixedly connected to the positions, located on one sides of the heating water outlet nozzles, in the connecting frame, water blowing heads are fixedly connected to the bottoms of the water blowing pipes, and sterilizing lamps are fixedly connected to the positions, located on the sides, away from the heating water outlet nozzles, of the bottoms of the inner walls of the connecting frame. The high-temperature water bath sterilization device has the beneficial effects that the high-temperature water bath can ensure that the temperature and the time required by sterilization are reached, microorganisms such as bacteria, viruses and spores are effectively killed, residual moisture on the surface or inside a sterilized article is avoided, the possibility of secondary pollution is reduced, and a sterile environment is ensured.

The invention relates to a method for increasing the sporulation of bacteria such as Bacillus velezensis and Paenibacillus polymyxa using a liquid medium comprising glucose, ammonium sulfate, magnesium chloride and non-ionic surfactant polymer compounds. This method for increasing sporulation is used in a method for generating a large quantity of spores and selecting spores more resistant to higher temperatures than the spores of wild strains. The spores with higher heat resistance than those of the wild strains can be used in a method for eliminating contamination in fermentations.

The invention discloses a clean bench of a sterile filling machine, and relates to the technical field of filling machines, the clean bench comprises a support, a filling bench is arranged in the support, a base is fixedly arranged at the bottom of the filling bench, the support is fixedly connected to the bottom of the base, a T-shaped cylinder is fixedly arranged at the top of the filling bench, a clamping device is fixedly arranged on the inner side wall of the T-shaped cylinder, and a filling port and a capping device are slidably arranged at the top of the support. Overflowed water flows into the water collecting tank through the filter screen, in order to avoid bacterium breeding caused by accumulated water and ensure that the clean bench is in a sterile environment, the clean bench is sterilized through the ultraviolet lamp, and the ultraviolet lamp can sterilize flowing water and prevent bacterium breeding in the water collecting tank; the filter screen is fixed through the bolts, the filter screen can be quickly disassembled only by disassembling the bolts during disassembly, and the device not only can prevent water accumulation, but also can ensure that no bacteria breed, and makes up for the defects of an existing device.

The present invention discloses a sterile bag-making device, which includes a machine platform. The strain tank has multiple processing chambers and a seed mixing chamber; one side of the feeding chamber is communicated with the seed mixing chamber; the bag-making boxes are respectively arranged at the bottom of the docking box, and a bag-making mechanism is arranged in the bag-making boxes. The other end of the discharge pipe is communicated with the sterile temporary storage chamber in the machine platform; the stirring mechanism is arranged in the seed mixing chamber. The matrix material after seed mixing is output into the bag-making boxes through the stirring mechanism in the seed mixing chamber, and sterile bag-making is carried out through the bag-making mechanism. By using the stirring mechanism in the strain tank, the present invention can perform a sterile bag-making action on the matrix material after seed mixing, without the need for an additional packaging machine, without relying on an external sterile environment, and can achieve sterile bag-making based on the sterile environment of the strain tank. It has an integrated structure of automatic tightening and cutting, higher bag-making efficiency, fewer transfer times, integrated integration, higher efficiency, and effectively reduces economic costs.

The invention discloses a planting method for improving the soil quality of a Chinese fir forest by cultivating ganoderma lucidum in bags. The planting method comprises the following steps: selecting a Chinese fir forest land planted for more than 5 years; adding water into sawdust, wheat bran and lime according to a mass ratio of 180: 40: 3, uniformly mixing, filling into a plastic barrel bag to prepare a mushroom stick, and sterilizing; inoculating the mushroom sticks with ganoderma lucidum strains in a sterile environment by adopting a two-end inoculation mode; carrying the inoculated mushroom sticks into a culture field, and carrying out crisscross stacking culture for 30-40 days; ditching between lines of the cunninghamia lanceolata, removing the plastic tube bags from the cultured ganoderma lucidum sticks, putting the ganoderma lucidum sticks into the ditches, and covering soil; and a step of ganoderma lucidum emergence management. The fungus stick substrate takes agricultural and forestry organic wastes (such as wood chips and bran) which can be quickly degraded as main components, and can be continuously mineralized and decomposed in the growth process of ganoderma lucidum mycelia, so that a large amount of water-soluble organic carbon, nitrogen and other mineral nutrients can be released to soil in a short time, and the fertility of surface soil is remarkably improved.

The invention discloses a sterile environment pilose antler mushroom liquid inoculation machine, and relates to the technical field of pilose antler mushroom inoculation, the sterile environment pilose antler mushroom liquid inoculation machine comprises an inoculation machine body, the inoculation machine body is provided with an auxiliary mechanism, the auxiliary mechanism comprises a servo electric cylinder and four clamping grooves, one end of the telescopic end of the servo electric cylinder is provided with a mounting plate, and the clamping grooves are formed in the mounting plate; two symmetrical driving motors are mounted at the bottom of the mounting plate, and a connecting piece is mounted at the output end of each driving motor; by arranging the auxiliary mechanism, the velvet antler mushroom liquid inoculation machine in the sterile environment has the functions of automatically taking down and covering a bottle cap of a culture bottle, so that the labor amount of workers is reduced, the labor time of the workers is shortened, the using effect of the velvet antler mushroom liquid inoculation machine in the sterile environment is improved, and the working efficiency of the workers is improved. Meanwhile, the use efficiency of the pilose antler mushroom liquid inoculation machine in the sterile environment is also improved.

The invention belongs to the field of natural compounds, and particularly relates to a frog follicle crude extract and an extraction method and application thereof. The specific technical scheme is as follows: the crude extract is from frog follicles of rana poxing and does not contain egg granules. The preparation method comprises the following steps: extracting follicles of frogs, removing egg granules in a sterile environment, adding a buffer solution, carrying out ultrasonic treatment, centrifuging, taking supernatant, and filtering to obtain the crude extract. The crude extract obtained by the invention keeps good activity, and can be further developed for subsequent application. The crude extract obtained by the invention can obviously reduce the surface tension, and has better antioxidant activity at the same time. As a natural product source product has good biocompatibility, the crude extract provided by the invention has good application prospects in multiple fields such as the cosmetic water retention field, the food preservation field and the pharmaceutical field based on the activity and the function.

The invention provides an open type tissue culture method of bamboo reed, which comprises the following tissue culture steps: S1, preparation of a gradient antibacterial culture medium: (1) preparation of the gradient antibacterial culture medium: an MS culture medium, 0.5 mg / L 6-BA and 0.1 mg / L NAA are added, 0.1% of tea polyphenol aqueous extract is added, and 0.03% of water-soluble chitosan oligosaccharide is added. Traditional chemical disinfection is replaced with a natural gradient antibacterial system, the dual functions of pollution prevention and control and root protection are achieved by combining a symbiotic bacterium slow release technology, and the stress resistance and lignification characteristics of plants are synchronously improved through the synergistic effect of salinity gradient stimulation and environmental signal molecules; a light-gas intelligent regulation and control system is developed to simulate the optimal growth condition, and a transplanting survival guarantee system is constructed in cooperation with a mycorrhiza pre-activation technology; the dependence of traditional tissue culture on a sterile environment is broken through, and the seedling culture efficiency, the ecological compatibility and the resource utilization level are comprehensively improved under an open condition.

The invention discloses a lucid ganoderma cultivation method which comprises the following steps: S1, preparation of a culture medium: weighing coffee grounds, tea leaf residues, bran, sawdust, perlite, charcoal, cane sugar and gypsum, and uniformly stirring the components in proportion; s2, sterilization: performing normal-pressure sterilization; s3, sterile inoculation: uniformly spreading strains on the surface of the culture medium in a sterile environment; s4, mycelium culture management: keeping the temperature, the air humidity and the ventilation quantity of a culture room, and culturing in a dark place; s5, management in a sporocarp formation period: when a small amount of brown primordia appear, improving air humidity, illumination intensity and ventilation quantity for culture; and S6, harvesting sporocarp. According to the ganoderma lucidum cultivation method, sufficient and appropriate nutrients can be provided for growth of ganoderma lucidum, the breathability of the culture medium is improved, hypha growth is accelerated, and the ganoderma lucidum yield is increased; the adsorption of autotoxic substances generated by continuous cropping of the ganoderma lucidum is enhanced, the influence of heavy metals on the ganoderma lucidum is reduced, the cultivation environment is improved, and the quality of the ganoderma lucidum is improved.

The invention relates to the technical field of mushroom stick inoculation machines, and provides a mushroom stick inoculation machine which comprises a rack, an inoculation device, a discharging device, a drilling device, a limiting device, a spraying disinfection device, an air purifier, a distribution box and a mushroom stick transferring device, the inoculating device comprises an inoculating stick arranged on the mushroom stick transferring device and a discharging device located at the top of the inoculating stick, and the inoculating stick is transferred and sequentially passes through the spraying and brushing disinfection device, the drilling device and the inoculating device. The spraying disinfection device, the drilling device, the inoculation device and the mushroom stick transferring device are cooperatively integrated on the same rack, so that full-process automatic continuous operation of mushroom stick disinfection, drilling and inoculation is realized. The spraying disinfection device disinfects the surfaces of the mushroom sticks in real time before the mushroom sticks enter the drilling station, clean air is continuously conveyed to the top operation area in combination with the air purifier, and the sterile environment is doubly guaranteed.

The invention relates to a plant, which can be operated in a plurality of operating modes, for filling objects in a sterile environment, said plant comprising a filling station, a station arranged downstream of the filling station, a freeze dryer, a first transport device (2) for transporting objects from the filling station towards the second station, a second transport device (3) for transporting objects to the freeze dryer, and a transfer device (4) arranged at a junction point between the first transport device (2) and the second transport device (3), wherein, in a first operating mode, objects can be transported from the filling station to the freeze dryer, in a second operating mode, objects can be transported from the freeze dryer towards the second station, and in a third operating mode, objects can be transported from the filling station towards the second station, wherein the first transport device comprises a linear transport path (20), wherein the junction point is provided on the linear transport path (20), wherein the transfer device (4) can be configured selectively in a rest state and in at least one working state, wherein, in the rest state, objects can be transported by means of the first transport device (2) such that the objects pass the junction point and, while passing the junction point, remain within the first transport device (2). The invention also relates to a method for filling and sealing objects.

This invention discloses a loose mulberry leaf bud Fu brick tea and its preparation method. The method comprises: picking mulberry leaf buds, spreading them out, fixing them, and drying them to make mulberry leaf bud tea; extracting, filtering, and concentrating Anhua black tea to make a concentrated liquid; mixing 100 parts of mulberry leaf bud tea with 20-50 parts of the concentrated liquid, adjusting the moisture content of the mulberry leaf bud tea, and then piling it up; after drying, wrapping it in a sterile environment and then treating it at a short time with high temperature; mixing Fu brick tea with sterile water and boiling it three times, the first two times at 70-85℃ and the third time at 90-100℃, filtering it in a clean environment after each boiling, and continuing to boil the residue; combining the filtrates and concentrating them under reduced pressure to make a glaze; piling the glaze with the short-time high-temperature treated mulberry leaf bud tea in a sterile environment at 15-25℃ and 20%-60% humidity, then fermenting it in an environment with 50%-80% relative humidity for 7-12 days, and then drying it to obtain loose mulberry leaf bud Fu brick tea.

The invention discloses an open-type cold region Chinese rose tissue culture seedling propagation method, which is characterized in that parachlorometaxylenol and miconazole nitrate are used as bacteriostatic agents and are respectively applied to tissue culture links such as explant earlier-stage treatment, appliance sterilization, culture medium preparation, explant disinfection, tissue culture seedling transplanting and the like, so that the propagation process of the cold region Chinese rose tissue culture seedling gets rid of environmental limitation. Explant disinfection does not need a strict sterile environment, a high-pressure sterilization pot and an ultra-clean workbench are not used, and rapid breeding of Chinese rose tissue culture seedlings is achieved. Compared with a conventional sterilization method, the induction rate of the cluster buds is remarkably improved, and the pollution rate, the vitrification rate and the browning rate are remarkably reduced. The growth vigor of the tissue culture seedlings after rooting is obviously improved; and particularly, the effects of Hanjin No.1 and Changbai Mountain vine roses are prominent.