946results about "Biochemistry apparatus" patented technology

Platforms, systems, and methods for genetic generalization in synthetic biology development. According to one aspect, there is provided a method for predicting performance associated with genetic edits, the method comprising: receiving, by a platform, information about a strain of a microorganism, wherein the information about the strain comprises information describing a plurality of genetic edits to a base strain of the microorganism; generating, by the platform, a set of genetic embeddings based on the information about the strain, wherein the generating comprises processing the information about the strain using one or more embedding models, wherein each of the one or more embedding models: receives the information about the strain of the microorganism as input; and applies computational transformations to the input using a corresponding embedding model to generate a multi-dimensional vector representation for each of the plurality of genetic edits.

A calibrator bottle and method of calibration. The calibrator bottle includes a plastic material that is doped or compounded with a fluorescent material system that fluoresces in a target range and may be used to calibrate a blood culture instrument that measures sample fluorescence to determine bottle positivity or negativity. Also described herein is a system and method to control the temperature of samples in a blood culture apparatus using a resistance temperature device (RTD) device that measures the temperature of the sample. The sample measurements are correlated with the ambient temperature to determine an ambient temperature that will provide a target bottle content temperature.

Disclosed herein are devices and systems for detecting microbial activity. A device (100) for detecting microbial contamination comprises a light source (20) that is configured to be optically coupled to an optical element (10), the optical element comprising a waveguide (2) and a diffraction grating (4), that together support a plurality of guided mode resonances at selected wavelengths of light from the light source. The device also comprises a condensing wall (40) having a condensing surface (50) on which liquid in gas (vapour) can condense, wherein the condensing surface is shaped, or engineered, to support flow of condensed liquids under gravity towards a collecting point (55) where the liquid can pool (6) around the optical element. The device further comprises a detector (30) configured to detect a property of a resonant guided mode of the plurality of resonant guided modes thereby to detect the presence or absence of microbes in the liquid that pools around the optical element.

A linear peristaltic pump (200) for a bioprocessing system comprises: at least one pair of jaws formed by a first jaw (201) and a second jaw (202), the first jaw (201) being moveable relative to the second jaw (202) such that the pair of jaws can be moved between an open configuration in which the jaws are spaced apart for receiving a flexible tube (155) therebetween and a closed configuration in which the jaws are brought together so as to retain the tube therebetween; an actuator mechanism configured to control movement of the first jaw relative to the second jaw; and means (220a-220m) for effecting a peristaltic pumping action on a fluid contained within the tube (155) when the tube is retained between the pair of jaws in the closed configuration.

Devices and methods for a microplate lid configured to cover a microwell assay plate are provided. A microplate lid includes a lid panel surrounded by a lid frame. Sidewalls extend downwardly from lid frame. The microplate lid includes various features to improve performance, including features to improve stickability, reduce warping, reduce sample contamination, reduce wear, reduce sample evaporation, facilitate robotic handling, and provide other benefits.

A device for receiving samples in a microscope is provided. The device comprises a microtiter plate. The microtiter plate comprises an inner part with cavities for the samples and with an optically transparent bottom plate, which seals the cavities underneath and a frame which defines a supporting surface and is configured to hold the inner part at least in a first position. The bottom plate is arranged above the supporting surface in the first position of the inner part. The inner part is movable relative to the frame along a direction (A) perpendicular to the supporting surface.

Methods of assembly of and a mixing system for use in a process bottle for mixing its contents, the mixing system including a low volume magnetically-driven mixer mounted at the bottom of the bottle. The mixer may have vanes and lower grooves, or no vanes and grooves on both upper and lower faces. The mixer has a “microsized” three-dimensional solid inanimate body to enable insertion through relatively small mouth openings at the top of conventional reactor bottles. The methods of assembly involve passing the microsized mixer through an open mouth of a process bottle and coupling the mixer with a bearing assembly at the floor of the bottle. The bearing assembly includes fixtures sealed around a hole in the floor of the bottle. A cap may be assembled to the bottle in a sterile environment to from a consumable assembly which is then enclosed in a sterile package for shipping to a customer. A variety of tube connectors to the cap may be customized for instant use by the customer.

A method for reaction injection molding of a structured component is disclosed. The method includes (i) closing a mold in order to provide a cavity adapted to form the component, wherein the mold includes a negative shape of the component, (ii) injecting a reaction injection molding resin at a low packing pressure into the cavity in order to fill the cavity, (iii) curing the reaction injection molding resin in the cavity using UV-light through a transparency in the mold, (iv) opening the mold, and (v) demolding the cured component. Also, a corresponding component, apparatus and system are disclosed.

Systems and methods are provided for providing a timeline representing a culture media protocol for a culture medium. Providing a timeline representing a culture media protocol can include receiving the culture media protocol for the culture media generating the timeline on a user interface based on the culture media protocol, monitoring time on the timeline, receiving one or more culture media images related to the culture media protocol, associating each of the one or more culture media images with a position on the timeline that correlates to a time at which the culture media image was captured, and generating a selectable marker for each culture media image associated with the timeline, the selectable marker being aligned with the position on the timeline that correlates to the time at which the culture media image was captured.

According to one aspect, there is provided an AI-based platform which may include an experiment data set including records that respectively represent an experiment. Each record may indicate at least one hypothesis associated with the experiment and an experiment definition based on the at least one hypothesis. An AI-based agent may be configured to perform an evaluation of respective records of each experiment, and generate, based on the evaluation, at least one observation about the at least one hypothesis associated with the experiment represented by each of the respective records.

The present technology relates to a systems for quantifying rheotaxis in a sperm-containing sample. The system includes a microfluidic system having an inlet for charging fluids into a passage. One or more probes defining a confinement region suitable for retaining motile sperm are within the passage. The system further comprises an image processing computing device for obtaining a sequence of images of the confinement region of at least one of the one or more probes having motile sperm retained therein. The sequence of images of the confinement region is processed to determine a signal intensity value for said sequence of images, wherein said signal intensity value is based on a concentration of the motile sperm located in the confinement region at the flow rate. A rheotaxis quality value is determined for said sperm-containing sample based on the signal intensity value. Methods for quantifying rheotaxis in a sperm-containing sample are also disclosed.

Materials, methods, and systems are provided for cell redifferentiation and expansion of induced pluripotent stem cell (iPSC)-derived hematopoietic stem cells (iHSCs) without the use of serum or additional cells.

A method and apparatus for calibrating an imaging system provides a program stored in the imaging system having a graphical user interface and providing a plurality of tools to align hardware components of the imaging system including cameras for imaging cell culture vessels received by the imaging system and having a tool for providing a video image output of a cell culture vessel received in the imaging system.

Systems and methods related to microfluidic devices (e.g., microfluidic devices comprising layers of films) are generally described. In some embodiments, a microfluidic device comprises a substrate configured to facilitate fluid transport, one or more intermediate layers disposed on the substrate, wherein the one or more intermediate layers are configured to define a plurality of fluidly connected microfluidic components, and a top layer disposed on the one or more intermediate layers. In certain embodiments, a microfluidic device comprises a microfluidic channel having a gap or area of increased hydrophobicity in between two separated portions of the channel to separately pin one or more liquids in one or more desired portions of the channel. According to some embodiments, a microfluidic device comprises a microfluidic channel with an inclined surface, such that different portions of the microfluidic channel are associated with different channel heights.

The disclosure relates to a mixture of bacteria belonging to at least six or seven different and specific bacterial species preferably for use in preventing or treating gastro-intestinal disorders. Preferably, the mixture of bacteria is grown together in a fermenter prior to administering the mixture to a subject in order to prevent or treat the disorder.

An apparatus may include a microfluidic chip including at least one microfluidic channel and a first at least one electrical interconnect. An apparatus may include a circuit board having at least one interface interconnect and a second at least one electrical interconnect electrically coupled with the first at least one electrical interconnect of the microfluidic chip. An apparatus may include a cartridge enclosing the microfluidic chip and the circuit board, the cartridge including at least one opening allowing access to the at least one microfluidic channel on the microfluidic chip and the at least one interface interconnect.

Methods of preparing cell cultures (e.g. tumor spheroids), methods of evaluating a therapeutic agent for cancer, and methods of cancer treatment.

To provide a method for separating and purifying extracellular vesicles.SOLUTION: Wherein the hollow fiber membrane has an inner diameter of 0.2 mm to 1.4 mm and a molecular weight cut-off of 100,000 to 1,000,000, and the filtration method comprises a first filtration step of pressurizing and filtering a culture supernatant of mesenchymal stem cells through a first opening on one end side of the hollow fiber membrane to separate the culture supernatant into a permeated liquid and a first concentrated liquid, and a second filtration step of pressurizing and filtering the first concentrated liquid through a second opening on the other end side of the hollow fiber membrane; A method for separating and purifying extracellular vesicles, the method comprising a second filtration step of separating the extracellular vesicles into a permeate and a second concentrate, wherein a concentrate having an increased concentration of the extracellular vesicles is obtained by alternate tangential flow filtration in which the first filtration step and the second filtration step are alternately performed a plurality of times, and a membrane surface speed in the first filtration step and the second filtration step is 0.3 to 2m / sec.SELECTED DRAWING: None

The present invention relates to a microfluidic device (10) suitable for accommodating an aqueous liquid, the device having essentially a planar shape, which device comprises at least one conduit (11) fluidically connecting at least two structures selected from compartments, reservoirs (14) and / or ports (15, 62). The conduit comprises at least one bend (12) describing an angle of between ≥85° and ≤275° around an axis orthogonal to the planar shape. The planar shape is arranged in an angle of between +45° and −45° relative to the direction of the force of gravity (G), or a centrifugal force (Fc) applied to the microfluidic device.

A system, method, and platform for target detection, the system including: a base substrate; a set of sample processing regions defined at a broad surface of the substrate, wherein each of the set of sample processing regions includes: a set of microwell subarrays arranged in a gradient between an upstream end and a downstream end of each respective sample processing region, and a boundary separating each respective sample processing region from adjacent sample processing regions; and a cover substrate configured to mate with the base substrate in a coupled mode, the cover substrate comprising a network of venting channels aligned with the set of sample processing regions upon mating the base substrate with the cover substrate in the coupled mode, the network of venting channels providing gas exchange between the base substrate and an environment surrounding the microwell assembly. The invention(s) can be used for MPN assays.

The invention relates to a computer-implemented method (400) performed by a controller (C) configured to control a bioprocess included in a bioreactor (BR), the method comprising: obtaining (410) measurement results by performing spectrophotometric measurements of a bioprocess fluid (FL); generating (420) control parameters based on the measurement results and a model; and controlling (430) the bioprocess using the generated parameters.

Methods, systems, and computer program products are provided for monitoring a cell culture, utilizing a predictive model to improve cell culture processes, measuring cell dynamics of a cell culture, managing cell clusters in a cell culture system, and training a machine learning model for managing cell clusters in a cell culture system. In various embodiments, a cell culture may be maintained in a cell culture chamber. The cell culture may comprise one or more cell colonies. One or more properties of the cell culture may be measured. One or more perturbations may be applied to all or a portion of the cell culture. The one or more properties of the cell culture may be measured after applying the one or more perturbations. The one or more properties may be compared before and after the one or more perturbations. The cell culture may be managed based on the comparison.

The present disclosure is drawn to inertial pumps. An inertial pump can include a microfluidic channel, a fluid actuator located in the microfluidic channel, and a check valve located in the microfluidic channel. The check valve can include a moveable valve element, a narrowed channel segment located upstream of the moveable valve element, and a blocking element formed in the microfluidic channel downstream of the moveable valve element. The narrowed channel segment can have a width less than a width of the moveable valve element so that the moveable valve element can block fluid flow through the check valve when the moveable valve element is positioned in the narrowed channel segment. The blocking element can be configured such that the blocking element constrains the moveable valve element within the check valve while also allowing fluid flow when the moveable valve element is positioned against the blocking element.

The present disclosure relates to systems and methods for monitoring liquid levels within cartridges of automated cell processing systems during cell processing. A method for automated cell processing may first include coupling a cell processing cartridge to an instrument configured to perform a cell processing operation with at least one module of the cartridge. Next, the method may include detecting, via a vision system of the instrument, a liquid level of a liquid within one or more cell processing modules of the cartridge. Finally, the method may include determining a volume of the liquid based on the liquid level.

Provided herein are devices and methods for improved oxygenation of biological cells transplanted into a biological subject. The devices and methods may be used to promote vascularization to a transplantation site of the biological subject, and / or may be used to improve survival and functionality of cells contained within an encapsulation device.

A plaque detection method and apparatus wherein at least one processor is programmed to receive above focus images to detect the presence of live cells without detecting the lysed cell materials, receive below focus images wherein virtual dark regions exist which are similar to cell shadows as seeds in a segmentation process and use contours around each resulting shape to obtain a subset that are more likely to be part of the cell population to define a cell map. A distance map is created in which each pixel value is the distance of that pixel from the nearest pixel of the cell map and the distance map is thresholded to create a first image of the places which are relatively far from the cells a second image with a smaller distance threshold to get an image that mimics the edges of the cells.

A bacterial colony forming unit (CFU) detection system is disclosed that utilizes a thin film transistor (TFT)-based image sensor array that saves about 12 hours compared to methods approved by Environmental Protection Agency (EPA). Lensless imaging modes are constructed using TFT image sensors having a sample field of view of about 10 cm2. And automatically collecting delay images of bacterial colonies cultured on the chromogenic agar plate every 5 minutes. Two deep neural networks are used to detect and count colonies in growth and identify their species. When 265 escherichia coli and other coliforms (namely citrobacter and klebsiella pneumoniae) colonies are used for blind detection, the average CFU detection rate of the system reaches 97.3% when the system is cultured for 9 hours, and the average recovery rate of the system reaches 91.6% when the system is cultured for about 12 hours. The TFT-based sensor can be applied to various microbiological detection methods. The imaging field of view of the platform can be economically and efficiently increased to more than 100cm < 2 >.

A structured assembly of perpendicular, interwoven fluidic conduits permits connections between conduits to be readily (and in some cases visibly) established, thereby providing operational convenience and amenability to automated means of validation or verification.

Subject-matter of the present invention is an improved process for processing a biological sample, in particular a process comprising the rotation around its own axis of said biological sample.