638results about "Biochemistry apparatus" patented technology

Platforms, systems, and methods for genetic generalization in synthetic biology development. According to one aspect, there is provided a method for predicting performance associated with genetic edits, the method comprising: receiving, by a platform, information about a strain of a microorganism, wherein the information about the strain comprises information describing a plurality of genetic edits to a base strain of the microorganism; generating, by the platform, a set of genetic embeddings based on the information about the strain, wherein the generating comprises processing the information about the strain using one or more embedding models, wherein each of the one or more embedding models: receives the information about the strain of the microorganism as input; and applies computational transformations to the input using a corresponding embedding model to generate a multi-dimensional vector representation for each of the plurality of genetic edits.

A calibrator bottle and method of calibration. The calibrator bottle includes a plastic material that is doped or compounded with a fluorescent material system that fluoresces in a target range and may be used to calibrate a blood culture instrument that measures sample fluorescence to determine bottle positivity or negativity. Also described herein is a system and method to control the temperature of samples in a blood culture apparatus using a resistance temperature device (RTD) device that measures the temperature of the sample. The sample measurements are correlated with the ambient temperature to determine an ambient temperature that will provide a target bottle content temperature.

Disclosed herein are devices and systems for detecting microbial activity. A device (100) for detecting microbial contamination comprises a light source (20) that is configured to be optically coupled to an optical element (10), the optical element comprising a waveguide (2) and a diffraction grating (4), that together support a plurality of guided mode resonances at selected wavelengths of light from the light source. The device also comprises a condensing wall (40) having a condensing surface (50) on which liquid in gas (vapour) can condense, wherein the condensing surface is shaped, or engineered, to support flow of condensed liquids under gravity towards a collecting point (55) where the liquid can pool (6) around the optical element. The device further comprises a detector (30) configured to detect a property of a resonant guided mode of the plurality of resonant guided modes thereby to detect the presence or absence of microbes in the liquid that pools around the optical element.

A linear peristaltic pump (200) for a bioprocessing system comprises: at least one pair of jaws formed by a first jaw (201) and a second jaw (202), the first jaw (201) being moveable relative to the second jaw (202) such that the pair of jaws can be moved between an open configuration in which the jaws are spaced apart for receiving a flexible tube (155) therebetween and a closed configuration in which the jaws are brought together so as to retain the tube therebetween; an actuator mechanism configured to control movement of the first jaw relative to the second jaw; and means (220a-220m) for effecting a peristaltic pumping action on a fluid contained within the tube (155) when the tube is retained between the pair of jaws in the closed configuration.

A device for receiving samples in a microscope is provided. The device comprises a microtiter plate. The microtiter plate comprises an inner part with cavities for the samples and with an optically transparent bottom plate, which seals the cavities underneath and a frame which defines a supporting surface and is configured to hold the inner part at least in a first position. The bottom plate is arranged above the supporting surface in the first position of the inner part. The inner part is movable relative to the frame along a direction (A) perpendicular to the supporting surface.

Methods of assembly of and a mixing system for use in a process bottle for mixing its contents, the mixing system including a low volume magnetically-driven mixer mounted at the bottom of the bottle. The mixer may have vanes and lower grooves, or no vanes and grooves on both upper and lower faces. The mixer has a “microsized” three-dimensional solid inanimate body to enable insertion through relatively small mouth openings at the top of conventional reactor bottles. The methods of assembly involve passing the microsized mixer through an open mouth of a process bottle and coupling the mixer with a bearing assembly at the floor of the bottle. The bearing assembly includes fixtures sealed around a hole in the floor of the bottle. A cap may be assembled to the bottle in a sterile environment to from a consumable assembly which is then enclosed in a sterile package for shipping to a customer. A variety of tube connectors to the cap may be customized for instant use by the customer.

According to one aspect, there is provided an AI-based platform which may include an experiment data set including records that respectively represent an experiment. Each record may indicate at least one hypothesis associated with the experiment and an experiment definition based on the at least one hypothesis. An AI-based agent may be configured to perform an evaluation of respective records of each experiment, and generate, based on the evaluation, at least one observation about the at least one hypothesis associated with the experiment represented by each of the respective records.

The present technology relates to a systems for quantifying rheotaxis in a sperm-containing sample. The system includes a microfluidic system having an inlet for charging fluids into a passage. One or more probes defining a confinement region suitable for retaining motile sperm are within the passage. The system further comprises an image processing computing device for obtaining a sequence of images of the confinement region of at least one of the one or more probes having motile sperm retained therein. The sequence of images of the confinement region is processed to determine a signal intensity value for said sequence of images, wherein said signal intensity value is based on a concentration of the motile sperm located in the confinement region at the flow rate. A rheotaxis quality value is determined for said sperm-containing sample based on the signal intensity value. Methods for quantifying rheotaxis in a sperm-containing sample are also disclosed.

Materials, methods, and systems are provided for cell redifferentiation and expansion of induced pluripotent stem cell (iPSC)-derived hematopoietic stem cells (iHSCs) without the use of serum or additional cells.

A method and apparatus for calibrating an imaging system provides a program stored in the imaging system having a graphical user interface and providing a plurality of tools to align hardware components of the imaging system including cameras for imaging cell culture vessels received by the imaging system and having a tool for providing a video image output of a cell culture vessel received in the imaging system.

Systems and methods related to microfluidic devices (e.g., microfluidic devices comprising layers of films) are generally described. In some embodiments, a microfluidic device comprises a substrate configured to facilitate fluid transport, one or more intermediate layers disposed on the substrate, wherein the one or more intermediate layers are configured to define a plurality of fluidly connected microfluidic components, and a top layer disposed on the one or more intermediate layers. In certain embodiments, a microfluidic device comprises a microfluidic channel having a gap or area of increased hydrophobicity in between two separated portions of the channel to separately pin one or more liquids in one or more desired portions of the channel. According to some embodiments, a microfluidic device comprises a microfluidic channel with an inclined surface, such that different portions of the microfluidic channel are associated with different channel heights.

The disclosure relates to a mixture of bacteria belonging to at least six or seven different and specific bacterial species preferably for use in preventing or treating gastro-intestinal disorders. Preferably, the mixture of bacteria is grown together in a fermenter prior to administering the mixture to a subject in order to prevent or treat the disorder.

An apparatus may include a microfluidic chip including at least one microfluidic channel and a first at least one electrical interconnect. An apparatus may include a circuit board having at least one interface interconnect and a second at least one electrical interconnect electrically coupled with the first at least one electrical interconnect of the microfluidic chip. An apparatus may include a cartridge enclosing the microfluidic chip and the circuit board, the cartridge including at least one opening allowing access to the at least one microfluidic channel on the microfluidic chip and the at least one interface interconnect.

To provide a method for separating and purifying extracellular vesicles.SOLUTION: Wherein the hollow fiber membrane has an inner diameter of 0.2 mm to 1.4 mm and a molecular weight cut-off of 100,000 to 1,000,000, and the filtration method comprises a first filtration step of pressurizing and filtering a culture supernatant of mesenchymal stem cells through a first opening on one end side of the hollow fiber membrane to separate the culture supernatant into a permeated liquid and a first concentrated liquid, and a second filtration step of pressurizing and filtering the first concentrated liquid through a second opening on the other end side of the hollow fiber membrane; A method for separating and purifying extracellular vesicles, the method comprising a second filtration step of separating the extracellular vesicles into a permeate and a second concentrate, wherein a concentrate having an increased concentration of the extracellular vesicles is obtained by alternate tangential flow filtration in which the first filtration step and the second filtration step are alternately performed a plurality of times, and a membrane surface speed in the first filtration step and the second filtration step is 0.3 to 2m / sec.SELECTED DRAWING: None

The invention relates to a computer-implemented method (400) performed by a controller (C) configured to control a bioprocess included in a bioreactor (BR), the method comprising: obtaining (410) measurement results by performing spectrophotometric measurements of a bioprocess fluid (FL); generating (420) control parameters based on the measurement results and a model; and controlling (430) the bioprocess using the generated parameters.

Methods, systems, and computer program products are provided for monitoring a cell culture, utilizing a predictive model to improve cell culture processes, measuring cell dynamics of a cell culture, managing cell clusters in a cell culture system, and training a machine learning model for managing cell clusters in a cell culture system. In various embodiments, a cell culture may be maintained in a cell culture chamber. The cell culture may comprise one or more cell colonies. One or more properties of the cell culture may be measured. One or more perturbations may be applied to all or a portion of the cell culture. The one or more properties of the cell culture may be measured after applying the one or more perturbations. The one or more properties may be compared before and after the one or more perturbations. The cell culture may be managed based on the comparison.

The present disclosure is drawn to inertial pumps. An inertial pump can include a microfluidic channel, a fluid actuator located in the microfluidic channel, and a check valve located in the microfluidic channel. The check valve can include a moveable valve element, a narrowed channel segment located upstream of the moveable valve element, and a blocking element formed in the microfluidic channel downstream of the moveable valve element. The narrowed channel segment can have a width less than a width of the moveable valve element so that the moveable valve element can block fluid flow through the check valve when the moveable valve element is positioned in the narrowed channel segment. The blocking element can be configured such that the blocking element constrains the moveable valve element within the check valve while also allowing fluid flow when the moveable valve element is positioned against the blocking element.

The present disclosure relates to systems and methods for monitoring liquid levels within cartridges of automated cell processing systems during cell processing. A method for automated cell processing may first include coupling a cell processing cartridge to an instrument configured to perform a cell processing operation with at least one module of the cartridge. Next, the method may include detecting, via a vision system of the instrument, a liquid level of a liquid within one or more cell processing modules of the cartridge. Finally, the method may include determining a volume of the liquid based on the liquid level.

Provided herein are devices and methods for improved oxygenation of biological cells transplanted into a biological subject. The devices and methods may be used to promote vascularization to a transplantation site of the biological subject, and / or may be used to improve survival and functionality of cells contained within an encapsulation device.

A plaque detection method and apparatus wherein at least one processor is programmed to receive above focus images to detect the presence of live cells without detecting the lysed cell materials, receive below focus images wherein virtual dark regions exist which are similar to cell shadows as seeds in a segmentation process and use contours around each resulting shape to obtain a subset that are more likely to be part of the cell population to define a cell map. A distance map is created in which each pixel value is the distance of that pixel from the nearest pixel of the cell map and the distance map is thresholded to create a first image of the places which are relatively far from the cells a second image with a smaller distance threshold to get an image that mimics the edges of the cells.

Subject-matter of the present invention is an improved process for processing a biological sample, in particular a process comprising the rotation around its own axis of said biological sample.

The present application provides a ship carbon dioxide tail gas treatment system, the system captures high concentration carbon dioxide captured by traditional CCS capture device, realizes carbon dioxide tail gas utilization electric conversion through electro-catalytic reduction technology, stores or directly discharges liquid organic product mainly in formic acid / formate, replaces existing liquefied storage CCS scheme, reduces energy consumption and storage space, and the product has higher economic value than liquid carbon dioxide.

A microtiter plate assembly is disclosed that includes a base having a plurality of wells and a lid having a plurality of projections corresponding to the plurality of wells. Each projection contains a radial notch and a canted distal tip, providing bubble free sealing, reduced oxygen back-diffusion, and increased sensitivity even at small sample volumes.

A particle analysis device can take a fluorescence observation mode in which fluorescence emitted by a fluorescent marker added to a particle or by the particle itself is imaged by irradiating the particle with excitation light, and a scattered light observation mode in which scattered light produced by irradiating the particle with the light is imaged. A particle specifying unit specifies the particle to which the fluorescent marker is added or the particle that emits the fluorescence from fluorescence image data obtained in the fluorescence observation mode. An analyzing unit analyzes physical properties of the particle by obtaining diffusion speed due to the Brownian motion of the particle specified by the particle specifying unit from the image data of the scattered light obtained in the scattered light observation mode having a frame number larger than that in the fluorescence observation mode.

A multilayer polymer film comprising an inner biomaterial contacting layer and an outer layer comprising a polymer selected from the group consisting of polyester elastomer copolymers, polyethylene terephthalate (PET), nylon, polyethylene, polypropylene, thermoplastic elastomers, and copolymers and mixtures thereof, wherein the difference between the melting temperature of the inner contact layer and the melting temperature of the outer layer is 30-120 DEG C.

One among various embodiments discloses a method to generate sub-pixel shifted images. The method includes generating light by sequentially activating each of a plurality of light emitting elements arranged in a spatially non-uniform configuration, propagating the generated light through a layer supporting a specimen, and generating a plurality of output signals from an image sensor configured to detect the light propagated through the layer supporting the specimen, the plurality of output signals indicative of a plurality of sub-pixel shifted images. A high-resolution image of the specimen can be generated based on the plurality of sub-pixel shifted images. The plurality of light emitting elements is locatable over a first range of separation distances with respect to the layer, and the layer is locatable over a second range of separation distances with respect to the image sensor.

A system may select a first feature and a second feature of the biologic product. The system may determine a first biologic parent having the first feature and not having the second feature, wherein the first feature is based on an aspect of the first biologic parent. The system may determine a second biologic parent having the second feature and not having the first feature, wherein the second feature is based on an aspect of the first biologic parent, and the aspect of the second biologic parent can be combined with the aspect of the first biologic parent. The system may determine a biologic product having the first feature and the second feature, wherein the biologic product is determined based on an evaluation of a set of combinations of the aspect of the first biologic parent and the aspect of the second biologic parent.

The present invention relates to a surface radiator (100) comprising a light-emitting semiconductor component (2) and a housing body (1). The housing body (1) has at least one cooling channel (10, 11) for coolant, which forms part of a fluid path (a), which extends from an inlet opening (3) to a return opening (4) on the housing body (1). An emission window (5) is arranged thereon, which overlies the light-emitting semiconductor component (2) and which defines a front side of the surface radiator (100) and which is transparent for an incident radiation (S) that can be emitted by the semiconductor component (2). The housing body (1) provides an attachment surface (12) spaced apart from the emission window (5) for the at least one light-emitting semiconductor component (2). The arrangement of the emission window (2) on the housing body (1) is formed in a fluid-tight manner and the housing body (1), the semiconductor component (2) and the emission window (5) delimit an emission chamber (6). The fluid path (a) is defined by i) at least one first cooling channel (10), which extends from the inlet opening (3) through the housing body (1) to an orifice opening (13), and ii) the emission chamber (6), and iii) at least one second cooling channel (11), which extends from the discharge opening (14) through the housing body (1) to the return opening (4). The coolant (K) is an electrically insulating liquid, which is transparent for the incident radiation (S).

Systems, devices, and methods for integrated cell processing and analysis with precise fluid management are disclosed. The system includes a cartridge with processing modules connected by a fluidic bus with pressure regulation to control fluid flow between modules and to analytical tools. Methods for controlling fluid transfer by adjusting pump operational parameters in response to pressure measurements ensure precise sample delivery volumes and flow rates throughout the integrated platform. Fluid pulsations are reduced or prevented during the fluid transfer for accurate sample transfers. Fluid transfer steps coordinate fluid flow between an on-cartridge analytical module and / or off-cartridge analytical tools. The cartridge may include an analytical module with a channel selector to direct fluid samples from a plurality of fluid conduits to selected analytical chips. A positioning system with rack-and-pinion mechanisms enables precise movement of analytical chips along multiple axes to interface with analytical tools.