302results about "Biochemistry apparatus" patented technology

Platforms, systems, and methods for genetic generalization in synthetic biology development. According to one aspect, there is provided a method for predicting performance associated with genetic edits, the method comprising: receiving, by a platform, information about a strain of a microorganism, wherein the information about the strain comprises information describing a plurality of genetic edits to a base strain of the microorganism; generating, by the platform, a set of genetic embeddings based on the information about the strain, wherein the generating comprises processing the information about the strain using one or more embedding models, wherein each of the one or more embedding models: receives the information about the strain of the microorganism as input; and applies computational transformations to the input using a corresponding embedding model to generate a multi-dimensional vector representation for each of the plurality of genetic edits.

According to one aspect, there is provided an AI-based platform which may include an experiment data set including records that respectively represent an experiment. Each record may indicate at least one hypothesis associated with the experiment and an experiment definition based on the at least one hypothesis. An AI-based agent may be configured to perform an evaluation of respective records of each experiment, and generate, based on the evaluation, at least one observation about the at least one hypothesis associated with the experiment represented by each of the respective records.

The present technology relates to a systems for quantifying rheotaxis in a sperm-containing sample. The system includes a microfluidic system having an inlet for charging fluids into a passage. One or more probes defining a confinement region suitable for retaining motile sperm are within the passage. The system further comprises an image processing computing device for obtaining a sequence of images of the confinement region of at least one of the one or more probes having motile sperm retained therein. The sequence of images of the confinement region is processed to determine a signal intensity value for said sequence of images, wherein said signal intensity value is based on a concentration of the motile sperm located in the confinement region at the flow rate. A rheotaxis quality value is determined for said sperm-containing sample based on the signal intensity value. Methods for quantifying rheotaxis in a sperm-containing sample are also disclosed.

A method and apparatus for calibrating an imaging system provides a program stored in the imaging system having a graphical user interface and providing a plurality of tools to align hardware components of the imaging system including cameras for imaging cell culture vessels received by the imaging system and having a tool for providing a video image output of a cell culture vessel received in the imaging system.

An apparatus may include a microfluidic chip including at least one microfluidic channel and a first at least one electrical interconnect. An apparatus may include a circuit board having at least one interface interconnect and a second at least one electrical interconnect electrically coupled with the first at least one electrical interconnect of the microfluidic chip. An apparatus may include a cartridge enclosing the microfluidic chip and the circuit board, the cartridge including at least one opening allowing access to the at least one microfluidic channel on the microfluidic chip and the at least one interface interconnect.

To provide a method for separating and purifying extracellular vesicles.SOLUTION: Wherein the hollow fiber membrane has an inner diameter of 0.2 mm to 1.4 mm and a molecular weight cut-off of 100,000 to 1,000,000, and the filtration method comprises a first filtration step of pressurizing and filtering a culture supernatant of mesenchymal stem cells through a first opening on one end side of the hollow fiber membrane to separate the culture supernatant into a permeated liquid and a first concentrated liquid, and a second filtration step of pressurizing and filtering the first concentrated liquid through a second opening on the other end side of the hollow fiber membrane; A method for separating and purifying extracellular vesicles, the method comprising a second filtration step of separating the extracellular vesicles into a permeate and a second concentrate, wherein a concentrate having an increased concentration of the extracellular vesicles is obtained by alternate tangential flow filtration in which the first filtration step and the second filtration step are alternately performed a plurality of times, and a membrane surface speed in the first filtration step and the second filtration step is 0.3 to 2m / sec.SELECTED DRAWING: None

The present disclosure is drawn to inertial pumps. An inertial pump can include a microfluidic channel, a fluid actuator located in the microfluidic channel, and a check valve located in the microfluidic channel. The check valve can include a moveable valve element, a narrowed channel segment located upstream of the moveable valve element, and a blocking element formed in the microfluidic channel downstream of the moveable valve element. The narrowed channel segment can have a width less than a width of the moveable valve element so that the moveable valve element can block fluid flow through the check valve when the moveable valve element is positioned in the narrowed channel segment. The blocking element can be configured such that the blocking element constrains the moveable valve element within the check valve while also allowing fluid flow when the moveable valve element is positioned against the blocking element.

A plaque detection method and apparatus wherein at least one processor is programmed to receive above focus images to detect the presence of live cells without detecting the lysed cell materials, receive below focus images wherein virtual dark regions exist which are similar to cell shadows as seeds in a segmentation process and use contours around each resulting shape to obtain a subset that are more likely to be part of the cell population to define a cell map. A distance map is created in which each pixel value is the distance of that pixel from the nearest pixel of the cell map and the distance map is thresholded to create a first image of the places which are relatively far from the cells a second image with a smaller distance threshold to get an image that mimics the edges of the cells.

The present application provides a ship carbon dioxide tail gas treatment system, the system captures high concentration carbon dioxide captured by traditional CCS capture device, realizes carbon dioxide tail gas utilization electric conversion through electro-catalytic reduction technology, stores or directly discharges liquid organic product mainly in formic acid / formate, replaces existing liquefied storage CCS scheme, reduces energy consumption and storage space, and the product has higher economic value than liquid carbon dioxide.

One among various embodiments discloses a method to generate sub-pixel shifted images. The method includes generating light by sequentially activating each of a plurality of light emitting elements arranged in a spatially non-uniform configuration, propagating the generated light through a layer supporting a specimen, and generating a plurality of output signals from an image sensor configured to detect the light propagated through the layer supporting the specimen, the plurality of output signals indicative of a plurality of sub-pixel shifted images. A high-resolution image of the specimen can be generated based on the plurality of sub-pixel shifted images. The plurality of light emitting elements is locatable over a first range of separation distances with respect to the layer, and the layer is locatable over a second range of separation distances with respect to the image sensor.

A system may select a first feature and a second feature of the biologic product. The system may determine a first biologic parent having the first feature and not having the second feature, wherein the first feature is based on an aspect of the first biologic parent. The system may determine a second biologic parent having the second feature and not having the first feature, wherein the second feature is based on an aspect of the first biologic parent, and the aspect of the second biologic parent can be combined with the aspect of the first biologic parent. The system may determine a biologic product having the first feature and the second feature, wherein the biologic product is determined based on an evaluation of a set of combinations of the aspect of the first biologic parent and the aspect of the second biologic parent.

Systems, devices, and methods for integrated cell processing and analysis with precise fluid management are disclosed. The system includes a cartridge with processing modules connected by a fluidic bus with pressure regulation to control fluid flow between modules and to analytical tools. Methods for controlling fluid transfer by adjusting pump operational parameters in response to pressure measurements ensure precise sample delivery volumes and flow rates throughout the integrated platform. Fluid pulsations are reduced or prevented during the fluid transfer for accurate sample transfers. Fluid transfer steps coordinate fluid flow between an on-cartridge analytical module and / or off-cartridge analytical tools. The cartridge may include an analytical module with a channel selector to direct fluid samples from a plurality of fluid conduits to selected analytical chips. A positioning system with rack-and-pinion mechanisms enables precise movement of analytical chips along multiple axes to interface with analytical tools.

The present application relates to an American Society of Mechanical Engineers (ASME) Bioprocessing Equipment (BPE) compliant continuous stirred tank reactor (CSTR) array designed for continuous chemical, pharmaceutical, and biotechnological applications. Each array features two to five CSTRs incorporating a comprehensive recirculation and output flow system with a positive displacement pump, a three-way recirculation diverter valve, and control valves for precise management of output and waste allowing handling of solutions, suspensions, and mixtures of fluids, solids, and gases. Additional features support easy maintenance and real-time process monitoring, enhancing operational flexibility and control. This CSTR system is tailored to meet stringent industry standards and improve process efficiency.

A first unit (1) for treating a biological process liquid comprises a first side face (2), a second side face (3) and a front face (4) meeting the two side faces. The front face comprises: a plurality of valves (7) adapted to receive one or more legs (8) of the disposable flow path (6) and to act on the one or more legs (8) of the disposable flow path (6); optionally, one or more pumps (10) adapted to receive the one or more legs of the disposable flow path and to act on the one or more legs of the disposable flow path; optionally, one or more sensors (11) adapted to receive and measure one or more parameters in one or more legs of the disposable flow path; wherein the plurality of valves and optional pumps and sensors are vertically offset from each other to give a slope of at least 3.0 degrees from a horizontal plane (h) to one or more legs of the disposable flow path received by the valves and optional pumps and sensors.

The invention provides for methods of differentiating pancreatic endocrine cells into pancreatic beta cells expressing PDX1, NKX6.1, MAFA, UCN3 and SLC2A. These pancreatic beta cells may be obtained by step-wise differentiation of pluripotent stem cells. The pancreatic beta cells exhibit glucose-dependent mitochondrial respiration and glucose-stimulated insulin secretion similar to islet cells.

To provide an oxygen reduction method for improving the preservation of whole blood and blood components. [Solution] The present invention provides a method for collecting blood and blood components that yields whole blood and blood components with reduced oxygen levels. The present invention provides a method for rapidly preparing deoxygenated blood and blood components for storage, which improves the overall quality of transfused blood and improves patient health outcomes.

Platforms, systems, and methods for pathway optimization for process bottlenecks in synthetic biology development. According to one aspect, there is provided a method of optimizing a biologic synthesis process, comprising: identifying at least one bottleneck in the biologic synthesis process; evaluating a set of variants of the biologic synthesis process; and selecting an adjusted biologic synthesis process, wherein the adjusted biologic synthesis process includes at least one variant of the set of variants that reduces the at least one bottleneck of the biologic synthesis process.

Devices and methods for a microplate lid configured to cover a microwell assay plate are provided. A microplate lid includes a lid panel surrounded by a lid frame. Sidewalls extend downwardly from lid frame. The microplate lid includes various features to improve performance, including features to improve stickability, reduce warping, reduce sample contamination, reduce wear, reduce sample evaporation, facilitate robotic handling, and provide other benefits.

Method and control system are provided for controlling values of process parameters of a pretreatment process of wood particles. A sampler is used to obtain a sample of a product flow of said pretreatment process after said wood particles have undergone steam explosion in a hemihydrolysis reactor. A particle measurement device is used to measure one or more characteristics of particles in said sample and to produce one or more pieces of measurement information indicative of the measured characteristics. Said one or more pieces of measurement information are used to select one or more values of one or more of said process parameters.

A sterilization indicator reading apparatus is provided. The reading apparatus includes a housing including a top portion defining an aperture therethrough. The aperture is dimensioned to at least partially receive a sterilization indicator therethrough, such that the sterilization indicator is at least partially received within the housing. The reading apparatus further includes a heating coil disposed within the housing. Upon insertion of the sterilization indicator within the housing through the aperture, the heating coil is spaced apart from the sterilization indicator. The heating coil is configured to radiatively heat the sterilization indicator. The reading apparatus further includes an air supply unit disposed within the housing and configured to selectively supply air to the heating coil.

A method for detecting at least one analyte (110) in a sample (112) is disclosed. The method comprises the following steps: a) providing at least one sample (112) having at least one analyte (110); b) incubating the sample (112) with microparticles (118) having at least one surface (120) whereby the analyte (110) is adsorbed on the surface (120) of the microparticles (118) and an analyte-microparticle-complex (122) is formed; c) providing at least one fiber sheet material (128) having at least one tip (130) and contacting the tip (130) of the fiber sheet material (128) to the sample (112) comprising the analyte-microparticle-complex (122), whereby at least the analyte-microparticle-complex (122) is sucked into the tip (130) of the fiber sheet material (128); d) contacting the tip (130) of the fiber sheet material (128) to a port of an analytical device; e) adding an extracting solvent (138) to the fiber sheet material (128) and ap¬ plying an electrical voltage to the fiber sheet material (128) whereby ions of the analyte (110) are generated and are expelled from the tip (130) of the fiber sheet material (128); and f) detecting the at least one analyte (110) with the analytical device (134).

An analytical method of and system for monitoring cell status whereby excitation energy is focused to an excitation cytometry volume in a cell sample to fluoresce first and second fluorophores of a cell. Fluorescence signals from the first and second fluorescing cell fluorophores are directed to a detection subsystem. A first peak in fluorescence intensity for the first fluorophore is detected as is a second peak in fluorescence intensity for the second fluorophore. The fluorescence signals are processed to identify when the first and second peaks occur substantially simultaneously indicating the presence of a cell at the excitation cytometry volume instead of background fluorescence and in response, the cellular status of the cell is measured using the intensity of the levels of the first and second peaks.

This specification provides an improved method for producing Fc-containing proteins. The method generally comprises culturing mammalian cells that produce Fc-containing proteins at a first pH setting for a first period of time, and then culturing the cells at a second pH setting higher than the first pH setting for a second period of time.

The invention discloses a pig UBC promoter and application thereof, and belongs to the field of gene engineering. The pig endogenous UBC promoter is separated and identified, it is determined through cell tests that the pig endogenous UBC promoter can drive an exogenous gene to be efficiently, stably and widely expressed in pig tissue cells, and the problems that in the transgenic pig product preparation process, the exogenous promoter possibly faces gene silencing, expression inconsistency and low expression efficiency are solved. The porcine endogenous UBC promoter has practical application value in preparation of transgenic pigs.

Platforms, systems, and methods for model-in-the-loop synthetic biology. According to one aspect, there is provided an AI-based platform, comprising: an experiment data set defining a synthetic biology experiment based on a model of a biologic process; and an AI-based agent configured to, generate an experiment definition for the synthetic biology experiment based on the model of the biologic process, cause the synthetic biology experiment to be performed based on the experiment definition, perform an evaluation of at least one outcome of the synthetic biology experiment, and update the model of the biologic process based on the evaluation.

A buffer management system includes a supply of concentrated buffer solution, an inline dilution skid having a manifold in fluid communication with the supply of concentrated buffer solution and a control unit configured to operate the dilution skid to selectively dilute the supply of concentrated buffer solution in the manifold with a source of water for injection (WFI), and a biocontainer assembly. The biocontainer assembly includes a biocontainer bag defining a storage volume and a fill level sensor configured to generate a fill level signal indicative of the amount of material within the storage volume of the biocontainer bag. The biocontainer bag is in fluid communication with the manifold of the dilution skid for storing diluted buffer solution therein. The fill level sensor communicates the fill level signal to the control unit of the dilution skis, which uses it to manage the operation of the dilution skid.

The present application provides a method for developing a basal culture medium, a method for developing a basal culture medium formulation and system thereof. The method for developing a basal culture medium comprises, (1) determining a regression model for selected culture indicators to predict the culture indicators of a basal culture medium; (2) acquiring an addition range of each component in the basal culture medium, and enumerating and randomly selecting to generate a plurality of candidate basal culture medium formulations; (3) predicting the culture indicators by adopting the regression model and recommending a basal culture medium formulation; and (4) performing cell culture experiments to verify the culture indicators of the recommended basal culture medium formulation.

An imaging apparatus comprising a fluorescence microscope (115), an imaging sensor (120) an optical module (110) and a phase lamp (125) is provided to facilitate epifluorescent imaging of three (or more) color channels and to perform phase contrast and / or bright field imaging of samples without manual adjustment of the imaging apparatus. This allows for automated imaging, over extended periods of time, of a plurality of samples by the imaging apparatus located inside an incubator (180) without disturbing the incubator environment to manually adjust the apparatus. Also provided are embodiments to facilitate user swapping of removable optical modules (110) and / or transillumination modules (125) to allow the imaging apparatus to be adapted to different combinations of assays and / or fluorescent indicators so as to increase the variety of experiments and / or fluorescent dyes that can be imaged using the imaging apparatus.

The present invention relates to systems and methods for inductively coupled plasma (ICP) analysis, including mass cytometry. Inductively coupled plasma (ICP) analyzers use an ICP torch to generate a plasma that atomizes and ionizes a sample. Analysis of the atomic ions can be performed by atomic analysis, such as mass spectrometry (MS) or atomic emission spectrometry (AES). Particle-based ICP analysis includes analysis of particles, such as cells, beads, or laser ablation plumes, by atomizing and ionizing the particles in an ICP torch prior to atomic analysis. In mass cytometry, the mass tags of the particles are analyzed by mass spectrometry, such as ICP-MS. The systems and methods of the present application include one or more of an ICP load coil including annular fins, particle suspension sample introduction fluidics, and an ICP analyzer.