29results about "Biochemistry apparatus" patented technology

The invention relates to a system for determining cellular respiration and nutrient metabolism of living cells or tissues, the system comprising: a cell container configured to receive and maintain a biological sample; a gas volume in fluid communication with the cell container, the gas volume containing gaseous components produced by metabolic activity of the biological sample; an optical detection unit configured to detect a target gas species in the gas volume by wavelength-selective absorption spectroscopy, the optical detection unit being further configured to distinguish between different isotopic species of the target gas species, the optical detection unit comprising an illumination source and detector arrangement optically coupled to the optical detection unit and configured for measurement of absorption signals corresponding to different isotopes of the target gas species; and an electronic control and evaluation unit operatively connected to the optical detection unit, the electronic control and evaluation unit being configured to acquire and process absorption data, determine absolute values and / or temporal variations of the target gas species concentration and isotopic composition, and output respiration-related parameters of the biological sample. In this way, a system is provided that enables precise and efficient determination of carbon dioxide produced by living cells or tissues under physiological conditions.

To provide a technique for suppressing functional variations between a plurality of neural cell-containing spheroids across a plurality of lots or within a single lot.SOLUTION: The present invention relates to a cell-containing vessel comprising a plurality of neural cell-containing spheroids for which the variation calculated by the calculation method described below is less than 20%, where the neural cell-containing spheroids contain a plurality of types of cells including neural cells. (Calculation Method) Calcium transient assays are conducted for each of a plurality of neural cell-containing spheroids, a number of spontaneous oscillations per 10 minutes is measured, an average and a standard deviation for the number of spontaneous oscillations are calculated, and a variation is calculated by the following formula (1). Variation (%)=standard deviation for number of spontaneous oscillations / average number of spontaneous oscillations×100.SELECTED DRAWING: None

Apparatus and associated methods relate to a fermentation monitoring apparatus configured to generate a visual indicia in a fluid well in response to pressure inside a vessel exceeding a predetermined pressure. In an illustrative example, the fermentation monitoring apparatus may include a vessel cap that includes a fluid well holding a quantity of fluid, and at least one fluid communication channel. The fluid well, for example, may receive gas released from the channel via the at least one fluid communication channel when the vessel cap is coupled to a first end of a vessel to define a chamber and a gas pressure in the chamber exceeds a predetermined threshold. Various embodiments may advantageously generate a visual indicium of gas bubbles in the quantity of fluid indicating to a user that the gas pressure in the chamber has exceeded the predetermined threshold.

The invention relates to a fluidic structure comprising a reservoir (14) and a channel (16), and to a method for the low-loss and gentle storage and transport of objects in a dispersion. The method comprises the steps of: providing a fluidic structure, introducing a mixture consisting of a carrier liquid, a shear-thinning medium and the objects dispersed therein into the reservoir (14), transporting at least some of the mixture from the reservoir (14) through the channel (16) at a flow rate, wherein the mixing ratio of the carrier liquid and the shear-thinning medium, and the flow rate in the fluidic structure are set such that, at rest or at a lower transport speed, the mixture has a sufficiently high viscosity which prevents the objects from sedimenting, and that, at a higher transport speed, the objects in the mixture are moved from the channel wall to the middle of the channel due to the shear thinning behaviour of the mixture and are transported further there.

A method for scaling data across different processes includes obtaining first time series data indicative of one or more input, state, and / or output parameters of a first process over time and second time series data indicative of one or more input, state, and / or output parameters of a second process over time. The method also includes generating a scaling model that specifies a time-varying scaling relationship of the input, state, and / or output parameters between the first and second processes, and using the scaling model to transfer source time series data associated with a source process to target time series data associated with a target process. The source time series data indicative of one or more input, state, and / or output parameters of the source process over time, and the target time series data indicative of the input, state, and / or output parameters of the target process over time. The method further includes storing the target time series data in a memory.

The present disclosure describes automated systems and methods for handling objects, such as culture plates or dishes. For example, in one embodiment, the present disclosure describes an automated stacker and de-stacker comprising a clamping mechanism, a lift pad, a pair of pins, and a cabinet. A stack of culture plates may be stored in the cabinet. During a stacking operation, the pair of pins may be raised to stop a culture plate traveling along a conveyor track. Once stopped, the culture plate may be raised above the conveyor track by the lift pad and clamped by the clamping mechanism. During a de-stacking operation, the clamping mechanism may be opened, and the culture plate may be lowered onto the conveyor track by the lift pad.

An imaging apparatus is provided that includes a fluorescence microscope (115), an imaging sensor (120), and an optical module (110) and phase light (125) to facilitate epifluorescence imaging of three (or more) color channels and to perform phase contrast and / or brightfield imaging of a sample without manual adjustment of the imaging apparatus. This allows for automated imaging of multiple samples over an extended period of time by the imaging apparatus located inside an incubator (180) without disturbing the incubator environment to manually adjust the apparatus. Embodiments are also provided that facilitate user replacement of the detachable optical module (110) and / or transillumination module (125) to allow the imaging apparatus to accommodate different combinations of assays and / or fluorescent indicators in order to increase the variety of experiments and / or fluorescent dyes that can be imaged using the imaging apparatus.

The present disclosure is drawn to microfluidic devices. The microfluidic device includes a microfluidic well, a layered composite stack, and an optical sensor. The layered composite stack includes an optical filter composited with an etch-stopping layer. The optical filter defines the microfluidic well. The optical sensor is associated with the microfluidic well and has the optical filter positioned therebetween.

A microfluidic chip for conducting microbiological assays, includes a substrate in which incubation segments, a sample reservoir and microfluidic channels connecting the sample reservoir with the incubation segments are arranged. The microfluidic chip further includes a non-aqueous liquid reservoir for containing non-aqueous liquid wherein the reservoir is connectable via a releasable airtight and liquid-tight valve with the microfluidic channels connecting the sample reservoir with the incubation segments each incubation segment comprises an incubation well connected by a gas-exchange channel to an unvented gas cavity.

The invention relates to a high-pressure viewing cell (1) for visual observation of a sample, in particular a microfluidic chip (2), wherein the sample can be arranged in a sample-receiving space (3) of the high-pressure viewing cell (1), wherein the high-pressure viewing cell (1) has a window element (5) for observing the sample through the window element (5) and a window-element holder (7) for holding the window element (5). To achieve a high level of operational capability, it is provided that, for the purpose of establishing a connection, in particular an interlocking and / or frictional connection, between the window-element holder (7) and the window element (5), the window element (5) is formed such that it at least partly tapers from an interior-pressure side of the window element (5) to an exterior-pressure side of the window element (5).

The anatomical model of a nasal cavity, such as a human nasal cavity, for in-vitro inhalation studies such as toxicological screening, intranasal drug delivery studies, and neurophysiological studies. The model includes a model body including separable upper and lower model portions together defining the nasal cavity and including fluidic channels therein that define an olfactory region of the upper model portion, and a nasal passage defined in the lower model portion. A biocompatible porous membrane is positioned between the upper and lower model portions, and the biocompatible membrane is configured for culturing olfactory epithelium cells thereon. An artificial mucous layer coats a surface of the nasal cavity and is configured to collect particles passing through the nasal cavity.

An analysis device according to the present invention comprises: at least one inspection device that inspects a specimen; and at least one specimen production device that produces a specimen. The analysis device is provided with a data processing unit that processes data. The inspection device and the specimen production device are each provided with a communication unit for communicating with each other. The specimen production device is provided with a control unit that controls the specimen production device and a production unit that produces a specimen. The inspection device is provided with a control unit that controls the inspection device and an inspection unit that inspects a specimen. The data processing unit calculates a value [number of specimens A] representing the number of specimens which have not been delivered to the inspection device and a value [number of specimens B] representing the sum of the number of specimens for which inspection can be additionally started in the inspection device and the number of specimens which are being inspected and the inspection of which will be completed in a prescribed time. The control unit of the specimen production device prohibits production of a new specimen in the specimen production device when the [number of specimens A] and the [number of specimens B] satisfy a first relationship.

What is described herein relates to automated aseptic method for formulation of cells comprising providing a container with a filling volume in the range of between of 0.01-50 ml, filling the container with a cell suspension and a solution, closing the container, mixing the cell suspension and the solution to generate a final suspension, wherein the container is a final product container

The present invention relates to hollow fiber tangential flow depth filtration media adapted for perfusion bioprocessing, and related TFDF filtration units, bioprocessing systems, and methods, including methods for perfusion cell culture. The hollow fiber elements of the present invention combine a relatively thin porous wall with a relatively low porosity to provide filtration media resistant to fouling during perfusion culture.

To allow a wireless sensor to measure properties of a substance and transmit the properties to a remote wireless receiver, to allow the wireless sensor to be fully enclosed within a container containing the substance, and to allow for remote monitoring of the properties of the substance without compromising integrity of a closed system.SOLUTION: In a method, a heating device includes a heating element and a wireless receiver; a submersible device includes a first temperature measuring element and a wireless transmitter; the wireless transmitter communicates with the wireless receiver; and the wireless receiver is located between the heating element and the submersible device. The method includes: measuring the liquid temperature of liquid using the first temperature measuring element; communicating the liquid temperature, from the first temperature measuring element to a heating element controller; and activating the heating element by the heating element controller based on the liquid temperature communicated to the heating element controller; and moving the submersible device in the liquid by magnetic force over a first magnet.SELECTED DRAWING: Figure 2

Provided are an imaging system and an imaging device capable of generating a super-resolution interference fringe image of an object to be observed flowing through a flow channel. A light source that irradiates light in a first direction and irradiates light toward a flow channel through which an object to be observed flows in a second direction orthogonal to the first direction, an imaging sensor that has an imaging surface orthogonal to the first direction and on which a plurality of pixels are two-dimensionally arranged in a manner non-parallel to the second direction and that images light passing through the flow channel to output an interference fringe image, and an information processing device that generates a super-resolution interference fringe image based on a plurality of interference fringe images output from the imaging sensor are included.

The present invention provides a chip for analyzing nucleic acid information of a biological sample, and a method for manufacturing the chip. The chip is applied to analyze spatial transcriptome information of a biological tissue sample. The present invention further provides a method for analyzing spatial transcriptome information of a biological tissue sample. The method and device of the present invention can effectively obtain expression information (including spatial omics information) of nucleic acids in cells of a tissue sample.

A well plate sealing apparatus according to the present invention comprises: a magazine for accommodating stacked sheets, a pickup unit for picking up the uppermost sheet in the magazine, a tray on which a well plate is disposed, a sheet transfer unit for transferring the picked-up sheet to a placing position, and a sealing unit for sealing wells of the well plate with the sheet, wherein the placing position is a position in which the picked-up sheet is placed on the well plate disposed on the tray.

A computer system and computer-implemented method are described for controlling and monitoring a process to produce a chemical, pharmaceutical or biotechnological product, the method comprising: providing a database, the database storing sets of process parameters to control and monitor respective ones of a plurality of processes performed in order to produce products, wherein each of the stored sets of process parameters is associated with a successful trajectory of a respective one of the processes performed according to the respective set process parameters, wherein each successful trajectory is a time-based profile of measurements recorded during performance of the respective process; receiving a set of characterizing process parameters that characterize the process; identifying a first set process parameters from the stored sets of process parameters, the first set of process parameters having a specified degree of similarity to the set of characterizing process parameters, wherein the first set of process parameters is associated with a first successful trajectory, controlling and monitoring the process using the first successful trajectory, comprising: recording measurements of the process; estimating a trajectory of the process based on the recorded measurements.

A cell evaluation method evaluates the quality of a cell population including a plurality of cells. The cell evaluation method comprises: an index calculation step of calculating an index, based on a captured image of the cell population, the index including at least any one of an average distance representing a packing degree of the cells, a spring constant representing a degree of consistency in distances between the cells, and a hexagonal order parameter representing a degree to which an arrangement of the cells resembles a regular hexagon; and an evaluation step of evaluating the cell population, based on the index calculated in the index calculation step.

A clinical sample testing system includes a portable apparatus for transport and incubation of a clinical sample in a culture vessel. The apparatus includes a sealable container having a thermally insulated compartment for receiving the vessel and a heater for heating the clinical sample to a temperature suitable for pre-culturing it. The system further includes a microorganism detection device which receives the vessel and cultures the clinical sample. The device includes a) a test aliquot extraction device for removing a test aliquot from the vessel and / or from a second culture vessel sampled therefrom, and a DNA testing device for separating DNA from the test aliquot and performing nucleic acid tests on the DNA to identify the microorganism and detecting genetic antimicrobial resistance markers therein and / or b) an antimicrobial susceptibility test device for performing an antimicrobial susceptibility test on the cultured clinical sample or portion thereof by monitoring microbial growth.

A system and method for detecting, enumerating, or extracting microorganisms in a sample is disclosed. Target microorganisms, such as Salmonella bacteria, may be of interest. Magnetic beads may be bound to the target microorganisms. After which, the bead-bound cells may be isolated. For example, a magnetic field may be applied in order to separate the target cells (with the magnetic beads attached thereto) and move then to a predetermined section of the well. Agar, or other immobilizing agent, may be added to the wells in order to immobilize the target cells. After which, the target cells are incubated and periodically analyzed to determine whether the target cells are growing, thereby indicating that the microorganisms are contained within the well.

The invention relates to a fluidic structure with a reservoir (14) and a channel (16) and a method for low-loss and gentle storage and transport of objects in a dispersion.The method comprises the following steps: providing a fluidic structure, introducing a mixture of a carrier fluid, a shear-thinning medium and the objects dispersed therein into the reservoir (14), transporting at least a part of the mixture from the reservoir (14) through the channel (16) at a flow rate wherein the mixing ratio of the carrier fluid and the shear-thinning medium on the one hand and the flow rate in the fluidic structure on the other hand are set such that the mixture at rest or at lower transport speeds has a sufficiently high viscosity to prevent the objects from settling, and that at higher transport speeds the objects in the mixture are moved from the channel wall to the center of the channel and transported further there due to the shear-thinning behavior of the mixture.

A system for evaluating a fluidic substance, the system comprising: a container carriage device configured to support one or more containers; a sample pipetting device configured to dispense a fluidic substance in at least one of the containers on the container carriage device; an image capture device configured to capture an image of at least one of the containers on the container carriage device; and at least one processing device; wherein the system is configured to: dispense, using the sample pipetting device, at least one fluidic substance into a container; capture, using the image capture device, an image of the container on the container carriage device; analyze, using the at least one processing device, the image of the container to determine a volume of the dispensed at least one fluidic substance in the container; and analyze, using the at least one processing device, the image of the container to determine a particle concentration of a total volume of fluidic substances in the container.

The present invention belongs to the field of production of recombinant proteins, particularly antibodies. More specifically, this relates to a method for producing recombinant proteins (such as antibodies) in a bioreactor and / or a method for enhancing cell culture ability during production of recombinant proteins in a bioreactor (N-stage) by applying organized operating stress to a seed culture through a specific feeding strategy in a seed bioreactor (N-1 stage), that is, by increasing the feeding rate, the feeding amount, and / or by increasing the power input by an impeller.

The present invention addresses the problem of providing: a cell culturing method whereby it becomes possible to prevent oxygen deficiency in cells when the cells are cultured at a high density; and a method for producing a useful substance utilizing the cell culturing method. According to the present invention, a cell culturing method is provided, in which the relationship among an agitation power per unit volume P / V, a dissolved oxygen concentration CL0 and a viable cell density VCD satisfies the requirement represented by formula 1 in cell culturing at a viable cell density of 60×106 cell / mL or more. Formula 1: 0.0059×exp[0.0243×(P / V)]×CL0 / VCD ≧ 1.10×10-17 In the formula, CL0 represents a dissolved oxygen concentration [mol / mL]; VCD represents a viable cell density [cell / mL]: and P / V represents an agitation power per unit volume [W / m3] which is defined by a specified formula.