28 results about "Antrodia" patented technology

The present invention discloses a fermentation method for Antrodia camphorata mycelium and an auxiliary composition for fermentation of Antrodia camphorata. The method provided by the present invention comprises the following steps: S1. subjecting an Antrodia camphorata strain to liquid fermentation for 60-120 hours; S2. adding precursors to the fermented Antrodia camphorata strain liquid fermentation broth, and continuing the fermentation for more than 120 hours to obtain Antrodia camphorata mycelium; the precursors being coenzyme Q0, p-hydroxybenzoic acid, linalool, and α-terpineol, and the fermentation broth after adding the precursors having a coenzyme Q0 content of 100-500 mg / L, a p-hydroxybenzoic acid content of 20-300 mg / L, a linalool content of 0.1-0.5 mg / L, and an α-terpineol content of 0.3-0.7 mg / L. This method adds precursor substances of specific composition and concentration to the liquid fermentation broth of Antrodia cinnamomea at a certain time and combines them with each other, which is beneficial to the secondary metabolism of Antrodia cinnamomea and promotes the synthesis of triterpenoid compounds, thereby increasing the yield. Compared with the existing technology, the yield of triterpenoid compounds produced by Antrodia cinnamomea mycelium is increased, the culture cycle is shortened, and it is more suitable for large-scale production.

The invention provides an antrodia camphorata sporocarp extract, which is prepared from the following raw materials in parts by weight: 20 to 24 parts of wild ganoderma lucidum extract, 8 to 12 parts of herba taraxaci and 8 to 12 parts of bolete. The antrodia camphorata sporocarp extract disclosed by the invention contains various physiologically active components such as triterpenoids, polysaccharides, superoxide disproportionated enzymes, adenosine, proteins (containing immune proteins), vitamins, trace elements, nucleic acid, lectin, amino acid, sterols, lignin, blood pressure stabilizing substances and the like; the traditional Chinese medicine composition can relieve various tumors such as hangover, gastrointestinal discomfort, heatstroke, hypertension, virus infection, diabetes mellitus, nephritis, liver cirrhosis and liver cancer, has the effects of resisting oxidation, resisting inflammation, inhibiting hypertension, protecting liver nerves, inhibiting tumor growth and the like, can bidirectionally regulate immunity and protect the liver, can also treat tumors at multiple targets, and has a wide application prospect. The discomfort of a patient in an anti-tumor process is reduced.

This invention discloses a culture medium for Antrodia camphorata mycelium, which consists of two parts: a solid medium and a liquid medium. The liquid medium contains glucose, peptone, anhydrous magnesium sulfate, and plant hormones; the plant hormones include one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. The solid medium contains carrot powder, potato powder, glucose, and agar powder; the solid medium also contains plant hormones, including one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. During cultivation, activated mycelium is first inoculated onto the solid medium to form high-quality colonies, and then inoculated onto the liquid medium for further expansion. This method significantly increases mycelium yield and the production of crude polysaccharides and crude triterpenes, providing strong technical support for the development and utilization of Antrodia camphorata.

The invention relates to application of terpenoids in treating or preventing fibrotic diseases. The application comprises the step of applying an effective amount of a composition to a subject in need of the terpenoids, wherein the composition comprises a triterpenoid compound extracted from antrodia camphorata or needle herb.

The invention provides application of antrodia camphorata polysaccharide in preparation of anti-hepatoma drugs, and relates to the technical field of biological medicines. The antrodia camphorata polysaccharide can regulate mononuclear cells to differentiate into macrophages, and promotes the classical activation of the macrophages through a cascade amplification effect to secrete proinflammatory factors, so that the proliferation of liver cancer cells is inhibited, and the anti-liver cancer effect is achieved. In addition, the antrodia camphorata polysaccharide is good in safety, free of toxic and side effects, capable of being used for preparing the medicine for treating the liver cancer, easy to produce, low in cost and remarkable in effect, and has great market development value and good application prospects.

The invention relates to a preparation method of a lotus leaf biological beverage. The preparation method comprises the following steps: 1) preparing 50-60 parts by weight of lotus leaf powder; 20-30 parts of Chinese wolfberry juice; uniformly mixing 20-30 parts of grape skin to obtain a mixed raw material; 2) inoculating the mixed raw material with lotus leaf lyophyllum commune bacteria and antrodia camphorata bacteria, and fermenting for 20-30 hours to obtain fermentation liquor; 3) after the fermentation is finished, filtering the fermentation liquid, and sterilizing the filtrate; and 4) adding bioactive peptide into the sterilized filtrate, and then sequentially performing homogenization and low-temperature drying treatment to obtain the lotus leaf biological beverage. The release efficiency of active ingredients in the lotus leaves can be remarkably improved through composite strain synergistic fermentation and an ultrasonic-assisted technology, so that the overall efficacy of the product is improved; meanwhile, through compounding of multiple raw materials and addition of natural components, the bitter taste of the lotus leaves is successfully improved, the unique flower and fruit fragrance is formed, and the palatability of the product is improved.

The present invention relates to a method of preventing or treating a fibrotic condition, comprising administering an effective amount of composition to a subject in need thereof; wherein the composition comprises triterpenes extracted from Antrodia-camphorate or Anisomeles indica.

The application provides application of a tremella fuciformis polysaccharide in preparation of an anti-liver cancer drug, and relates to the technical field of biological medicine.The tremella fuciformis polysaccharide in the application can regulate differentiation of monocytes into macrophages, promote classical activation of the macrophages through cascade amplification, and secrete proinflammatory factors, so that the proliferation of liver cancer cells is inhibited, and the effect of resisting liver cancer is achieved.In addition, the tremella fuciformis polysaccharide in the application has good safety, no toxic side effects, can guarantee that the tremella fuciformis polysaccharide is used for preparing a drug for treating liver cancer, is easy to produce, is low in cost, has remarkable effect, has great market development value and good application prospect.

The invention discloses a method for directionally converting a precursor substance in an antrodia camphorata crude extract into high-value triterpenoid antroquinonol in vitro by using recombinant yeast methylated transferase COQ5 and a mutant of the recombinant yeast methylated transferase COQ5. The method comprises the following steps: taking solid-state fermented or wild sporocarp as a raw material, and performing ethanol extraction to obtain a crude extract; in an oleic acid-adenosylmethionine-phosphoric acid buffer system, a wild type or mutant type crude enzyme solution is added, and a reaction is performed at 37 DEG C for 5-12 h, so that the content of antroquinonol can be increased by 2.0-2.6 times. Compared with a PBS blank group, the relative content of antroquinonol in the optimal mutant COQ5-V2 (H132D) catalytic group reaches 260%, and the reaction conditions are mild. The method is simple in process, low in cost and easy to amplify, and the technical bottlenecks that wild antrodia camphorata resources are deficient and the content of active ingredients is low can be solved.

This invention belongs to the field of Antrodia camphorata cultivation technology, specifically relating to a method for cultivating Antrodia camphorata that can increase the triterpenoid content, comprising the following steps: S1. Inoculating the Antrodia camphorata strain into a liquid fermentation medium for initial liquid fermentation culture to obtain initial mycelium; S2. Transferring the initial mycelium to a Cinnamomum camphora culture medium for growth culture to obtain Antrodia camphorata; during the liquid fermentation culture process, periodic ultrasonic treatment is performed, the frequency of the ultrasonic treatment is 35kHz to 60kHz, with a treatment cycle of 2min-3min followed by an interval of 1min-2min, and the treatment is performed for 4h-6h per day. This invention combines liquid fermentation culture with solid culture, which improves production efficiency. Simultaneously, during the liquid fermentation culture stage, periodic ultrasonic treatment stimulates and accelerates the metabolic process of Antrodia camphorata, thereby increasing the content of triterpenoid compounds.

The invention discloses a traditional Chinese medicine composition and application thereof in low-deuterium small molecular group compound wine. The traditional Chinese medicine composition is prepared from the following components in parts by mass: 40 parts of an anti-tumor compound, 50-70 parts of rhizoma polygonati, 50-75 parts of fructus lycii, 45-65 parts of herba cistanche and 2-7 parts of fructus ziziphi jujubae, the anti-tumor compound is prepared from antrodia camphorata and ganoderma lucidum according to the mass ratio of (2-4): 1. The traditional Chinese medicine composition and the small-molecular-group low-deuterium compound wine prepared from the traditional Chinese medicine composition have the advantages that the negative influence of traditional Chinese liquor on human health is remarkably relieved, and the effects of nourishing the liver, tonifying the kidney, regulating both the liver and the kidney, regulating blood sugar and blood fat, improving vascular permeability, improving immunity and inhibiting tumors are achieved for a liquor product through the traditional Chinese medicine composition and the small-molecular-group low-deuterium compound wine prepared from the traditional Chinese medicine composition; and 2) experiments show that the traditional Chinese medicine composition provided by the invention obviously improves the inhibition rate of natural traditional Chinese medicines on liver cancer HepG2 cells.

The invention provides an external traditional Chinese medicine composition for treating allergic rhinitis. The external traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 160 to 240 parts of paniculate swallowwort root, 36 to 54 parts of cicada slough, 0.24 to 0.36 part of borneol and 0.24 to 0.36 part of antrodia camphorata. The invention further provides a preparation method and application of the external traditional Chinese medicine composition. Pharmacodynamic tests prove that the medicinal effect of the traditional Chinese medicine composition is obviously superior to that of a prescription disclosed in the application number 201010238755.7, and the traditional Chinese medicine composition is more favorable for being prepared into a gel and exerts a better medicinal effect. The prepared external nasal drop can remarkably improve clinical symptoms of allergic rhinitis and is high in safety, meanwhile, the invention further provides a method for preparing the external nasal drop, and the method is simple in process and easy to produce.

The application belongs to the technical field of traditional Chinese medicines, and particularly relates to a preparation method and application of multi-effect Antrodia camphorata composite drop granules. The preparation method comprises the following steps: (1) preparation of Antrodia camphorata extract; (2) preparation of mixed extract of Aspidistra elatior and Schisandra chinensis; (3) preparation of mixed extract of Taraxacum mongolicum, Pueraria lobata and Poria cocos; (4) mixing of the Antrodia camphorata extract, the mixed extract of Aspidistra elatior and Schisandra chinensis, the mixed extract of Taraxacum mongolicum, Pueraria lobata and Poria cocos to obtain a mixed powder; (5) heating of polyethylene glycol to be completely melted into a liquid, addition of the mixed powder, uniform mixing, injection into a spherical mold, cooling into a pill, demolding, drying, and obtaining of the multi-effect Antrodia camphorata composite drop granules. The Antrodia camphorata extract prepared in the application has a high total triterpene content, the multi-effect Antrodia camphorata composite drop granules have a quick sobering effect, and simultaneously have a liver protection effect.

The invention discloses a method for preparing hydroxytyrosol-containing extract from antrodia camphorata and hydroxytyrosol, and belongs to the technical field of research and development of natural medicines. Antrodia camphorata is used as a strain, and hydroxytyrosol is obtained through microbial fermentation, product extraction, separation and purification. The extract containing hydroxytyrosol and hydroxytyrosol prepared by the invention can be used for developing antrodia camphorata health-care food or medicine with the effects of resisting oxidation, removing wrinkles, resisting aging, resisting cancer, resisting thrombus, regulating blood fat, resisting arteriosclerosis, resisting pathogenic microorganisms and the like. The antrodia camphorata culture medium is prepared by antrodia camphorata microbial fermentation, is wide in raw material source, simple in preparation method and easy to realize industrial production.

The invention discloses a method for inducing antrodia camphorata mycelia to generate triterpenoid components by utilizing Taiwan cinnamomum kanehirae calluses, and belongs to the technical field of agricultural biology. Taiwan cinnamomum kanehirae cells are obtained through tissue culture, then the Taiwan cinnamomum kanehirae cells and antrodia camphorata strains are co-cultured, and extraction analysis or separation and purification show that the method can induce antrodia camphorata mycelia to generate triterpenoid components. According to the method, the triterpenoid component with various biological effects can be obtained without cultivating the antrodia camphorata sporocarp, and the preparation process is simple and suitable for industrial production.

This invention provides a compound traditional Chinese medicine composition, its preparation method, and its application in the preparation of drugs for treating allergic rhinitis, belonging to the field of traditional Chinese medicine technology. The compound traditional Chinese medicine composition of this invention includes: Antrodia camphorata, Paeonia suffruticosa, Cynanchum paniculatum, Cicadae periostracum, Calculus bovis, Borneol, and Mentha haplocalyx. Antrodia camphorata, the principal ingredient, fundamentally regulates immunity and reduces inflammation and swelling. Paeonia suffruticosa, Cynanchum paniculatum, and Cicadae periostracum assist in anti-allergy and clearing heat and wind. Calculus bovis and Borneol are adjuvant ingredients that resolve phlegm, open the orifices, clear heat, and relieve pain. Mentha haplocalyx guides the medicine upwards and relieves local symptoms. This invention treats allergic rhinitis using a combination of internal and external application. Internal administration exerts a systemic regulatory effect and enhances immunity, while external application to the nose quickly relieves symptoms. The comprehensive formula addresses both the root cause and symptoms, with the various drug components synergistically enhancing anti-inflammatory, anti-allergic, and immunomodulatory effects to achieve the therapeutic effect of treating allergic rhinitis.

The invention relates to application of terpenoids in treating or preventing fibrotic diseases. The application comprises the step of applying an effective amount of a composition to a subject in need of the terpenoids, wherein the composition comprises a triterpenoid compound extracted from antrodia camphorata or needle herb.

The application discloses a characteristic nucleic acid molecule primer, a kit and a method for rapidly and quantitatively detecting Callophoma plicatus. The nucleic acid molecule primer is BSZ2F: 5'-CAAGTGCTCAACCGCTAC-3' and BSZ2R: 5'-TGCTGATTCTCCACGATG-3'. The application adopts a fluorescent quantitative PCR technology to detect, and the detection limit is as low as 0.03 ng / µL. The method is simple, specific, short in time and capable of being completed within 2 hours.

The invention provides a composition for repairing damaged skin cells and a preparation method thereof, and belongs to the technical field of skin repair, the composition comprises a glycyrrhiza glabra root extract, cordyceps cicadae extract peptide, a ganoderic acid-exopolysaccharide compound and a citrus aurantium extract. The glycyrrhiza glabra root extract is obtained by performing ultrasonic extraction and purification on root core coarse powder of glycyrrhiza glabra through a mixed solution containing choline chloride and lactic acid. The isaria cicadae miquel extract peptide is obtained by sequentially carrying out enzymolysis and purification on isaria cicadae miquel dry powder through chitinase, fig protease and serrapeptase. The ganoderic acid-exopolysaccharide compound is obtained by fermenting antrodia camphorata in a culture medium containing olive squalane to obtain a water phase and mycelia, performing enzymolysis on the mycelia through chitinase and beta-glucanase to obtain enzymatic hydrolysate, combining the enzymatic hydrolysate with the water phase, and performing purification. The citrus aurantium flower extract is obtained by performing water extraction and purification on citrus aurantium flower powder. All the components are matched and can accurately act on key pathological links of damaged skin cells, and the safe, mild and efficient repairing function is achieved.

The invention belongs to the technical field of biology, and particularly relates to a preparation method of antrodia camphorata ferment. According to the method, Trichoderma longibrachiatum and Bacillus licheniformis are mixed to prepare a composite fermentation seed solution, the composite fermentation seed solution is used for carrying out composite fermentation on a culture medium composed of cassava residues and wood chips, and the pretreated antrodia camphorata culture medium is prepared. Then, inoculating an antrodia camphorata strain into the culture medium for fermentation, and introducing a heat stress treatment step in the fermentation process; according to the method, synthesis and dissolution of triterpenoids and polysaccharide active ingredients of antrodia camphorata are remarkably promoted through combination of the synergistic effect of specific strains and physical stress, the technical problem that in the prior art, active substances of antrodia camphorata are not sufficiently dissolved out is solved through unique strain combination and process design, and the method has extremely high application value and market prospects.

This invention relates to a method for preventing or treating fibrotic conditions, comprising administering an effective amount of a composition to a subject in need; wherein the composition comprises a triterpenoid compound extracted from Antrodia camphorata or Gynostemma pentaphyllum.

Provided is a preparation method for a plate-cultured Antrodia cinnamomea active distinguishing product, comprising: carrying out twice extraction and filtration on plate-cultured Antrodia cinnamomea fruiting body powder by using alcohol, combining the two filtrates to obtain a mixed solution, and carrying out drying treatment on part of the mixed solution to obtain an Antrodia cinnamomea extract; and adsorbing the remaining mixed solution with macroporous resin, concentrating under reduced pressure until drying, separating by macroporous resin and a glass tube column, and eluting multiple times by using secondary water, alcohol, and ethyl acetate according to a preset mode to separately obtain first, second, third, and fourth Antrodia cinnamomea active distinguishing products. The Antrodia cinnamomea extract and the Antrodia cinnamomea active distinguishing products can be used for preparing a pharmaceutical composition for treating atopic dermatitis.

The invention relates to the technical field of natural products, and particularly discloses a composition, a preparation method of the composition and application of the composition in preparation of a product with a hangover alleviating effect. The composition comprises the following traditional Chinese medicine raw materials: 10-20 parts of hovenia dulcis thunb; 10 to 20 parts of ampelopsis grossedentata; 5-15 parts of radix puerariae; 5 to 15 parts of hawthorn; and 5-10 parts of licorice. The traditional Chinese medicine raw materials also comprise nutmeg, radix sophorae flavescentis, antrodia camphorata and / or flos puerariae; furthermore, the invention also provides another composition, and the composition is obtained by uniformly mixing the traditional Chinese medicine extract with the yeast extract, the corn peptide, the oyster peptide and the L-cysteine, wherein the weight ratio of the traditional Chinese medicine extract to the yeast extract to the corn peptide to the oyster peptide to the L-cysteine is 1: (8-9): (0.3-1): (0.3-1): (0.5-1.5). Researches show that the composition has the effect of dispelling the effects of alcohol; therefore, the compound has an important application value when being used as an active ingredient for preparing medicines or health-care products with the effect of dispelling the effects of alcohol.

The invention provides application of an antrodia camphorata extract. The antrodia camphorata extract has the effect of regulating and controlling a downstream path of signal transmission mediated by Nrf2 protein activation. Therefore, the antrodia camphorata extract can be used for preparing the pharmaceutical composition for regulating and controlling the Nrf2 protein, and has application potential in related markets.

This invention discloses a high-efficiency greenhouse cultivation method for *Antrodia camphorata* in the field of medicinal fungal cultivation technology, including strain activation, substrate preparation, inoculation culture, and fruiting management. The substrate contains wheat bran, corn flour, sucrose, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, and lanthanum-modified multi-level carbon-silicon composite nanomaterials, with vitamin B1 added after sterile filtration. After static cultivation in a greenhouse, the mycelium is induced to produce fruiting bodies through atomized spraying, and then dried after harvesting. The lanthanum-modified multi-level carbon-silicon composite nanomaterials are prepared by chitosan coordinating with lanthanum nitrate, precipitating with anhydrous ethanol, assembling with mesoporous silica, cross-linking with 3-aminopropyltriethoxysilane and tetraethyl orthosilicate under glacial acetic acid catalysis, and finally activated by gradient calcination and washing with anhydrous ethanol. This invention avoids the Maillard reaction and surface crusting during high-temperature sterilization, protects the activity of the lanthanum oxide crystal phase, significantly improves the mycelial growth rate and triterpenoid yield of *Antrodia camphorata*, and shortens the cultivation cycle.

The present invention provides a cultivation method for increasing the antrocinnamomin H content in Antrodia cinnamomea mycelium, relating to the technical field of edible and medicinal fungi production processes. The cultivation method comprises inoculating Antrodia cinnamomea mycelium into a liquid fermentation medium supplemented with Polygonum multiflorum powder for cultivation; the liquid fermentation medium is a WDF liquid fermentation medium, and the formula is 0.5g / 100mL pea powder, 0.1g / 100mL potassium dihydrogen phosphate, 0.05g / 100mL magnesium sulfate, 0.5g / 100mL yeast powder, 0.01g / 100mL VB1, and the balance is water. The present invention overcomes the shortcomings of the prior art by optimizing the fermentation medium formula and improving the fermentation conditions, thereby increasing the antrocinnamomin H content in Gaoligong Antrodia cinnamomea mycelium and enhancing the pharmacological activity of Gaoligong Antrodia cinnamomea mycelium.

The invention provides a compound traditional Chinese medicine composition, a preparation method thereof and application of the compound traditional Chinese medicine composition in preparation of medicine for treating allergic rhinitis, and belongs to the technical field of traditional Chinese medicine. The compound traditional Chinese medicine composition comprises antrodia camphorata, cortex moutan, paniculate swallowwort root, cicada slough, calculus bovis, borneol and mint. The monarch drug antrodia camphorata can fundamentally regulate immunity and resist inflammation and detumescence, the ministerial drugs cortex moutan, cynanchum paniculatum and cicada slough can assist in resisting allergy and dispelling wind and clearing heat, the adjuvant drugs calculus bovis and borneol can reduce phlegm, induce resuscitation and clear heat and relieve pain, and the envoy drug mint can guide the drugs upwards and relieve local symptoms. The traditional Chinese medicine is used for treating allergic rhinitis by adopting an oral administration and external application mode, plays a whole body regulating role and improves the immunity by oral administration, and is used for the nose to quickly relieve symptoms by external application. The traditional Chinese medicine composition treats both symptoms and root causes, all the medicine components are mutually compounded and synergized, the anti-inflammatory, anti-allergic and immunoregulation effects are enhanced, and the effect of treating allergic rhinitis is achieved.

The invention discloses a compound capable of inhibiting the activity of acetylcholin esterase and a preparation method thereof, and belongs to the field of research and development of natural medicines. The acetylcholin esterase inhibitor is obtained through antrodia camphorata microbial fermentation, product extraction, separation and purification. The inhibitor can be used for developing medicines for treating Alzheimer's disease or Alzheimer's disease, or can be used for developing anti-aging health-care foods and anti-oxidation products. The antrodia camphorata microbial fermentation preparation method is simple, the raw material source is wide, and industrial production is easy to realize.