566 results about "Antrodia" patented technology

A method for cultivating Antrodia Camphorata is provided. First, Antrodia Camphorata is pre-incubated on a sloped surface culture medium. A portion of the pre-incubated Antrodia Camphorata is transferred to a liquid culture medium for incubation. Then, Antrodia Camphorata incubated in the liquid culture medium is moved to a solid culture medium for incubation. The cultivated Antrodia Camphorata has similar efficacies for inhibiting cancel cell growth, reducing the occurrence of atherosclerosis or recovering the damaged liver functions, as the wild Antrodia Camphorata.

The present invention relates to a novel compound and its uses, which is an extract isolated and purified from Antrodia camphorate, in particular to 4-hydroxy-2,3 -dimethoxy-6-methyl-5(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone, and its use in tumor growth inhibition. The compound of the invention, which has never been discovered in Antrodia camphorate, can be applied in inhibiting the growth of cancer cells, such as breast cancer, hepatic cancer and prostate cancer; and be used as a pharmaceutical composition to inhibit the tumor growth; or further be applied in prevention of heart and blood vessel disease or dietary supplements for health needs through its antioxidant activity.

A solid state fermentation (SSF) method is provided which is effective for both small- and large-scale fungal cultivation. Also provided is SSF media for fungal cultivation. The media preferably contains a carbon source and nitrogen source to provide a carbon to nitrogen ratio of about 5:1 to about 25:1 by weight. The media may also contain a vitamin and an inorganic substance. A preferred SSF medium contains malt extract, yeast extract, peptone, glucose, water, solid base, and calcium carbonate or gypsum. Before propagating fungal mycelia in the SSF medium, the mycelia may be pre-cultivated in a solid culture medium and then in a liquid medium. Although the SSF method can be used in growing most fungi, preferred fungi include Cordyceps sinensis, Ganoderma lucidum, Antrodia camphorata, Trametes versicolor, and Agaricus blazei. The SSF method not only produces high yield of fungi, but also stimulates the production of fungal metabolites, particularly the kinds with pharmaceutical and medicinal activities. Cordyceps sinensis is preferably grown to produce the active compound H1A which is a derivative of ergosterol.

The present invention relates to a compound of Antrodia camphorata used to inhibit HBV, in particular to an extract, 4-hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone which is isolated from Antrodia camphorata, and its use in inhibiting HBV effectively. The cyclohexenone compound according to the present invention showed cytotoxicity on HBV-secreting human hepatoma cell line HepG2 2.2.15, decreased synthesis of HBV particles, further inhibited synthesis of HbsAg and HbeAg effectively to achieve the goal of HBV inhibition.

The invention relates to a 'micro ventilation lime wood antrodia camphorate cultivation method', and belongs to the field of fungus cultivation. The method is characterized by comprising: inoculating a liquid strain onto a camphorwood section with the moisture content of 50 to 70 percent; and performing micro ventilation culture to culture fruit bodies at a temperature of 25 to 28 DEG C and a humidity of 85 to 95 percent. In the method, camphorwood is treated, the liquid strain obtained by liquid fermentation and reproduction is inoculated onto the surface of camphorwood, culture is performed in a micro ventilation mode by controlling culture temperature, humidity, light and other conditions, the growth period is 11 months, the biological conversion rate is 15 percent, the polysaccharide content is equal to that of wild antrodia camphorate, the antrodia camphorate cultivated on the lime wood has the same appearance, fragrance, bitter taste, medicinal effectiveness and the like as wild antrodia camphorate.

The invention provides an antrodia camphorate cultivation method; firstly, pre-cultivating the antrodia camphorate on a slant culture medium, taking out a part of pre-cultivated antrodia camphorate to inoculate a liquid culture medium, and then inoculating the antrodia camphorate cultivated in the liquid culture medium to a solid culture medium for the cultivation. The antrodia camphorate cultivated in the method has similar effects of wild antrodia camphorate, including effects of restraining growth activity of human cancer cells and cancer auxiliary treatment, also having abilities of restraining human low density lipoprotein oxidation for lowering incidence rates of artery hardening and restoring damaged liver functions.

A solid culture method for antrodia camphora includes culturing mycelia with space bags and cultivating sporophores in air. The cultured product and its usage are also disclosed.

The invention relates to health preserving tea which is prepared from lohanguo siraitia fruit, boat-fruited sterculia seed, rose, red date, dwarf lilyturf root tuber, wolfberry fruit, aloe, licorice root, lotus plumule, eucommia bark, black tea, Americans ginseng, ganoderma lucidum spore powder, tuber fleeceflower root, poria cocos, Chinese yam rhizome, Cassia occidentalis, cassia seed, mulberry leaves, Lithocarpus polystachyus wild tea, raw hawthorn, Grifola frondosa and Antrodia camphorate. The health preserving tea has the effects of clearing and nourishing throat, invigorating qi, tonifying kidney, tranquilizing mind, improving sleeping, caring skin, removing toxic materials, losing weight, and the like, does not contain artificial sugar, and has sweet taste and esthetic color. The health preserving tea can effectively enhance immunity of human body, and has simple production process, convenience in consumption and moderate price, and is very suitable for modern sub-health people for conditioning and health preserving.

The invention discloses a novel cultural method of antrodia camphorata mycelium. In the culturing process of the cultural method, sesame oil is added into a fermentation tank culture medium which is used for replacing the existing chemical defoaming agent. The invention provides a novel natural defoaming agent, and fermentation liquid of the antrodia camphorata mycelium obtained by the cultural method leads the tank collecting days of the antrodia camphorata mycelium to be shortened and the dry weight thereof to be increased, and ensures the color of the antrodia camphorata mycelium to be similar to red of the natural antrodia camphorata; and the antrodia camphorata mycelium is rich in sesamol and has higher nutritional value compared with the antrodia camphorata mycelium cultured by the existing method.

Animal feedstuff having beneficial properties is obtained by adding to a substrate one or more fungal species of the kind that excretes substances into said substrate during its growth which are beneficial to the health, growth or weight gain of an animal, or animals to which the feedstuff is intended and allowing the fungus to grow and / or ferment on the substrate. Suitable substrates are cereal grains, residue of cereal grains, agricultural primary products, agricultural waste products, and other cellulosic materials or a combination of one or more of the same. Suitable fungus species include Cordyceps species, Ganoderma species, Grifola species, Trametes Species, Lentinula species, Antrodia species, Agaricus species, Tremella species, Pleurotus species, Lentinus species, Polypore species, Agaricales species, Ascomycetes species and Basidiomycetes species. Some substrates such as certain agricultural waste products and cellulosic material are not suitable for animal consumption per se but become suitable as a result the fungal growth and fermantation by the fungus or fungi on and in them.

The present invention relates to novel mixture and maleic and succinic acid derivatives from mycelium of Antrodia Camphorata and the medical use thereof. The present invention relates to the composition or mycelium comprising the compounds of the invention.

A preparation method for an ethanol extract of the fruiting body of Antrodia camphorata (EEAC) is provided. The preparation method includes steps of: (a) providing the fruiting body of A. camphorata (AC); (b) extracting the fruiting bodies with a first ethanol solution; and (c) obtaining EEAC. EEAC further can be sequentially extracted or fractioned by n-hexane, ethyl acetate and ethanol, and an n-hexane fraction (FC), an ethyl acetate fraction (FA) and an ethanol fraction (FB) respectively are generated. The growth inhibition and apoptosis induction of leukemia cell line HL 60 are effectively mediated by FA product, in which zhankuic acid A is the bioactive marker. The amount of triterpenoid in the fruiting body of AC can be determined by NMR and HPLC analysis.

The invention discloses an Antrodia camphorate mycelium fermentation extract abstracted by ethanol, wherein the Antrodia camphorata bacterial strain Ac001 was preserved in the China General Microbiological Culture Collection Center with a docket number of CGMCC No.1460. The Antrodia camphorate mycelium fermentation extract has appreciable actions in inhibiting hepatitis B virus HbsAg, e antigen HbeAg and HBV DNA secretion and resisting cancers especially liver cancer.

This invention relates to a new compound from Antrodia Camphorata and maleic acid of maleic acid and amber acid, and its medicine use. This invention relates to the mycelium and combination which includes compound in this invention.

The invention provides an Antrodia camphorate quick liquid fermentation process based on asexual spores. The process is characterized by comprising the following steps of: fermenting Antrodia camphorate serving as parent strains in a fermentation tank to generate the asexual spores; and taking a part of fermentation broth containing the Antrodia camphorate asexual spores to another fermentation tank provided with fresh fermentation media to continuously ferment after the completion of fermentation, wherein material removing and feeding, re-fermentation and circulation can be repeated several times according to requirements. Compared with a batch fermentation process, the quick fermentation process can shorten the fermentation period of the Antrodia camphorate by at least 40 percent and has the characteristics of improvement on production strength, reduction in the probability of bacterial contamination, stabilization of fermentation quality of an Antrodia camphorate product and the like.

A large-scale deep liquid fermenting process for preparing the mycelia of antrodia cinnamonea includes such steps as collecting the fungus of antrodia cinnamonea, liquid slant culture in shake flask, enlarging the culture, class-one seed culture, fermenting culture, and fermenting in (1000-5000)-L fermentor for 42 hr.

The present invention relates to a compound of Antrodia camphorata used for liver protection, in particular to an extract, 4-hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone which is isolated from Antrodia camphorata. The cyclohexenone compound according to the invention helps to alleviate liver injury and fibrosis induced by chemicals and reduces the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST). By increasing the contents of glutathione peroxidase (GSHPx) and catalase (CAT), cyclohexenone further decreases the liver damage and the oxidative pressure caused by free radicals, enhances the antioxidant ability and achieves the purposed of liver protection.

The invention discloses an antrodia camphorata extraction method, antrodia camphorata oral liquid and a preparation method of the antrodia camphorata oral liquid. An antrodia camphorata extractive in the antrodia camphorata oral liquid is prepared by taking antrodia camphorata sporocarp or mycelium as an antrodia camphorata raw material. The antrodia camphorata oral liquid extracted from the antrodia camphorata sporocarp and mycelium tends to be natural. In order to extract effective components of the antrodia camphorata to a maximum extent, the antrodia camphorata extraction method comprises the steps of performing hot water extraction and ethanol extraction for the antrodia camphorata raw material in sequence, then processing extraction solutions obtained by the two steps, and adding the extraction solutions into the antrodia camphorata oral liquid according to a technical science. In order to improve medical effects of the antrodia camphorata, effective substances such as beta-glucan, fucoidin, arabinose, and the like are further added into the antrodia camphorata oral liquid and used for cooperating with the medical effects of the antrodia camphorata, so that the absorption rate and the effects of the antrodia camphorate oral liquid are maximized.

A culture method of a fruiting body of Antrodia camphorata is provided, which includes: (a) fermenting a culture medium containing yeast at 5-35° C. for 3-30 days; (b) adding wood flour to the fermented culture medium with stirring; (c) placing the wood flour and the culture medium into a vessel; (d) sterilizing the vessel containing the wood flour and the culture medium; (e) inoculating Antrodia camphorata strains into the sterilized vessel containing the wood flour and the culture medium, and culturing at 5-35° C., to form mycelia; (f) inoculating the wood flour containing Antrodia camphorata mycelia into a wood segment; (g) placing the wood segment inoculated with Antrodia camphorata mycelia in an environment where the temperature is 5-35° C. and the humidity is 65-85%, and culturing for a period of time; and (h) removing the wood flour remained on the wood segment, then placing the wood segment in an environment where the temperature is 15-35° C. and the humidity is 80-98%, and culturing for a period of time, to form a fruiting body of Antrodia camphorata. A culture method of Antrodia camphorata mycelia, a culture method of a fruiting body of Antrodia camphorate, and a fruiting body of Antrodia camphorata cultured by the same method are also provided. The fruiting body of Antrodia camphorata cultured by the method of the present invention is thick and solid, and the content of triterpenoids is comparable to that of wild Antrodia camphorate.

The invention relates to the field of fermentation engineering, and discloses a method for preparing antrodia camphorata ferment. The method comprises the following steps of: a) performing combined fermentation on fruits and vegetables by using antrodia camphorata bacteria, bacillus subtilis, lactobacillus, microzyme and bacillus aceticus to obtain a ferment stock solution of the fruits and vegetables; b) performing combined fermentation on edible fungi and seaweeds by using the antrodia camphorata bacteria, the bacillus subtilis, the lactobacillus, the microzyme and the bacillus aceticus to obtain a ferment stock solution of the edible fungi and seaweeds; and c) combining the ferment stock solution of the fruits and vegetables with the ferment stock solution of the edible fungi and seaweeds, uniformly mixing, and then canning and ripening the mixture for more than 20 days to obtain a finished product of the antrodia camphorata ferment. By adopting the method for preparing the antrodia camphorata ferment, beneficial viable organisms are purposely added to accelerate fermentation, so that a food material is degraded or converted into micro-molecular easily-absorbable substances in a short time, bacterial contamination is reduced, the antrodia camphorata bacteria grow quickly, triterpene and polysaccharide type functional substances are effectively accumulated, and a lot of beneficial factors are generated through the multi-bacterium combined fermentation.

The present invention provides preparation process of antrodia camphorata polysaccharide and antrodia camphorata triterpene with adsorptive microporous resin and its product, and relates to chromatographic separation technology with adsorptive microporous resin and extraction of effective Chinese medicine component. Antrodia camphorata sporophore or mycelium is extracted with hot water and sectional eluted in microporous resin with water and alcohol to obtain antrodia camphorata polysaccharide and antrodia camphorata triterpene separately. The products of the present invention are used in preparing medicine and antrodia camphorata triterpene has excellent liver cancer cell resisting activity.

The invention discloses a new compound and the usage, in particular to a 4-hydroxy-2, 3- dimethoxy -6-methyl-5 (3, 7, 11- trimethyl-2, 6, 10-dodecatrien) -2- cyclohexenone (4-hydroxy- 2, 3-dimethoxy -6- methy-5 (3, 7, 11-trimethyl -dodeca-2, 6, 10- trienyl) -cyclohex-2-enone) separated from antrodia camphorate extract and the application in inhibiting the growth of tumor cell. The new compound is firstly seen in antrodia camphorate. The 4-hydroxy-2, 3- dimethoxy -6-methyl-5 (3, 7, 11- trimethyl-2, 6, 10-dodecatrien) -2- cyclohexenone (4-hydroxy- 2, 3-dimethoxy -6- methy-5 (3, 7, 11-trimethyl -dodeca-2, 6, 10- trienyl) -cyclohex-2-enone) separated from antrodia camphorate extract and the application in inhibiting the growth of tumor cell has the advantages of inhibiting the growth of breast cancer, liver cancer and prostate cancer tumor cell, being a component of drug combination inhibiting the growth of the tumor cell at the same time or further being applied in preventing cardiovascular disease or being added into healthy food by right of antioxidant activity.

The invention discloses a production method of antrodia camphorata stock seeds. The production method is characterized by comprising the steps of inoculating antrodia camphorata to an activation inclined culture medium for conventional culture and inoculating the antrodia camphorata to a stock seed culture medium for conventional culture to obtain the antrodia camphorata stock seeds, wherein Cinnamomum kanehirai chips contained in the conventional activation inclined culture medium are replaced by Cinnamomum camphora (L.) Presl. Chips in the activation inclined culture medium and Cinnamomum kanehirai chips contained in the conventional stock seed culture medium are replaced by Cinnamomum camphora (L.) Presl. Chips in the stock seed culture medium. According to the production method of the antrodia camphorata stock seeds, the Cinnamomum kanehirai is replaced by the common Cinnamomum camphora (L.) Presl. to produce the antrodia camphorata stock seeds and accordingly a new matrix is explored for the artificial cultivation of the antrodia camphorate to replace rare Cinnamomum kanehirai wood and the great significance to the artificial cultivation of the antrodia camphorate and the protection of Cinnamomum kanehirai resouces is achieved.

The present invention relates to a compound of Antrodia camphorata used to treat autoimmune diseases, in particular to an extract, 4-hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone, isolated from Antrodia camphorata, and its use in alleviating symptoms of autoimmune diseases such as systemic lupus erythematosus (SLE). The cyclohexenone compound according to the present invention helps to decrease proteinuria levels and antinuclear antibody titers in SLE mammals in order to alleviate kidney inflammation and disease, as well as the self-damage caused by antinuclear antibodies. The purpose for prevention and treatment of autoimmune diseases and kidney diseases by the natural, side-effect free substance can then be accomplished.

The invention aims at providing a mixed fungus ultrafine powder which reserves active ingredients maximumly, is easy to be absorbed and has multiple health care functions, and a preparation method thereof. The preparation method comprises the following steps: A. putting selected good Sparassia crispa fruit body, Trametes robiniophila Murr fruit body and Phellinus linteus fruit body into a vacuum freezing drying oven for drying, and slicing for standby; and putting liquid fermented Antrodia camphorate mycelium into the vacuum freezing drying oven, and drying for standby; B. respectively processing and grinding the four types of dry products obtained in the step A by a motor-driven grinder with 30-100 meshes of sieve mesh; C. processing and grinding coarse powders and Ganoderma spore obtained in step B by an ultrafine grinder with the 1000-2500 meshes of sieve mesh; and D. fully mixing the ultrafine powders obtained in step C by the following weight percent: 60-80% of Sparassia crispa fruit body ultrafine powders, 5-10% of Trametes robiniophila Murr fruit body ultrafine powders, 5-10% of Ganoderma lucidum karst spores, 5-10% of Phellinus linteus fruit body ultrafine powders, and 5-10% of Antrodia camphorate liquid fermentation mycelium ultrafine powders.

The invention relates to the field of fungus cultivation, and provides a method for cultivating antrodia camphorate sporocarp basswood in the second stage. The method includes: artificially assisting basswoods to decay; cultivating dominant bacteria of antrodia camphorate; transplanting the antrodia camphorate to the artificially assisted decayed basswoods after the antrodia camphorate grows into a dominant bacterial community, and obtaining the cultivating antrodia camphorate sporocarp. By the aid of the method for cultivating antrodia camphorate sporocarp basswood in the second stage, the problems about transformation rate and nutrition supplement of the antrodia camphorate to the basswoods are effectively solved, so that yield rate of antrodia camphorate cultivation is effectively increased by 4-8 times, cultivation time is shortened by 5-8 months, and industrialized cultivation mode of antrodia camphorate sporocarp basswood is effectively realized.

The present invention relates a process for culturing isolates of Antrodia camphorata to provide a product useful in medical and nourishment fields. The present invention also relates to a novel isolate of Antrodia camphorata capable of growing in a suitable artificial medium, while exhibiting desired pharmacological activities, in particular anti-tumor activity. The utilization of potato dextrose broth and the synthetic medium containing fructose as major carbon source leads to a significant increase in the pharmacological activity of the cultures of A. camphorata.

The invention discloses an antrodia camphorata anti-cancer active substance and a preparation method and application thereof. The antrodia camphorata anti-cancer active substance is prepared by the following steps of: (1) fermenting and culturing an antrodia camphorate mycelium with a liquid; (2) extracting the obtained mycelium with ethanol to obtain an ethanol extract; (3) concentrating the obtained ethanol extract, dissolving into the water, and adding an equal volume of acetic ether for extracting to obtain an acetic ether extract; (4) purifying the obtained acetic ether extract by performing colloid pipe column chromatography, and extracting an eluent segment g from a n-hexane / acetic ether gradient eluent as a mobile phase; and (5) purifying the obtained eluent segment g by performing colloid pipe column chromatography, and extracting a specific eluent segment from the n-hexane / acetic ether gradient eluent to obtain the antrodia camphorata anti-cancer active substance. The antrodia camphorata anti-cancer active substance provided by the invention is purified to a single component, and the effect of resisting cancer cells can be realized under the condition of very low dosage. The single component can be synthesized by using a chemical synthesis method, so that an anti-cancer effect is achieved.

The invention relates to a method for extracting antrodia camphorata oil by employing a CO2 supercritical extraction process. The method comprises: cleaning antrodia camphorata, quickly freezing at -35 DEG C, drying in vacuum to have a water content less than 2%, crushing by a refrigeration pulverizer, sieving, and performing supercritical CO2 extraction; controlling an extraction tank to have the temperature of 35 DEG C and the pressure of 25 MPa, and controlling the CO2 flow to be 35 L / h, and adding food-grade 96% ethanol as an entrainer; controlling a separation tank to have the temperature of 30 DEG C and the pressure of 10 MPa; and controlling a rectification column to have the temperature of 25 DEG C and the pressure of 12 MPa, so as to obtain antrodia camphorata oil. The antrodia camphorata oil obtained by extraction has the advantages of high effective compositions, no residual organic solvents and the like, the extraction technology is easy to operate, the extraction yield is high, and the product can be used as a raw material for producing traditional Chinese medicines and health products.

The present invention relates to a novel application of a compound. The compound 4-hydroxy-2,3-dimethoxy-6-methyl-5-(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone of the invention is isolated and purified from the extracts of Antrodia camphorata, which can be applied for inhibiting the survival of ovarian cancer cells and be used as a pharmaceutical composition to inhibit the ovarian tumor growth.