14 results about "Antrodia" patented technology

The invention provides an antrodia camphorata sporocarp extract, which is prepared from the following raw materials in parts by weight: 20 to 24 parts of wild ganoderma lucidum extract, 8 to 12 parts of herba taraxaci and 8 to 12 parts of bolete. The antrodia camphorata sporocarp extract disclosed by the invention contains various physiologically active components such as triterpenoids, polysaccharides, superoxide disproportionated enzymes, adenosine, proteins (containing immune proteins), vitamins, trace elements, nucleic acid, lectin, amino acid, sterols, lignin, blood pressure stabilizing substances and the like; the traditional Chinese medicine composition can relieve various tumors such as hangover, gastrointestinal discomfort, heatstroke, hypertension, virus infection, diabetes mellitus, nephritis, liver cirrhosis and liver cancer, has the effects of resisting oxidation, resisting inflammation, inhibiting hypertension, protecting liver nerves, inhibiting tumor growth and the like, can bidirectionally regulate immunity and protect the liver, can also treat tumors at multiple targets, and has a wide application prospect. The discomfort of a patient in an anti-tumor process is reduced.

This invention discloses a culture medium for Antrodia camphorata mycelium, which consists of two parts: a solid medium and a liquid medium. The liquid medium contains glucose, peptone, anhydrous magnesium sulfate, and plant hormones; the plant hormones include one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. The solid medium contains carrot powder, potato powder, glucose, and agar powder; the solid medium also contains plant hormones, including one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. During cultivation, activated mycelium is first inoculated onto the solid medium to form high-quality colonies, and then inoculated onto the liquid medium for further expansion. This method significantly increases mycelium yield and the production of crude polysaccharides and crude triterpenes, providing strong technical support for the development and utilization of Antrodia camphorata.

The invention relates to a preparation method of a lotus leaf biological beverage. The preparation method comprises the following steps: 1) preparing 50-60 parts by weight of lotus leaf powder; 20-30 parts of Chinese wolfberry juice; uniformly mixing 20-30 parts of grape skin to obtain a mixed raw material; 2) inoculating the mixed raw material with lotus leaf lyophyllum commune bacteria and antrodia camphorata bacteria, and fermenting for 20-30 hours to obtain fermentation liquor; 3) after the fermentation is finished, filtering the fermentation liquid, and sterilizing the filtrate; and 4) adding bioactive peptide into the sterilized filtrate, and then sequentially performing homogenization and low-temperature drying treatment to obtain the lotus leaf biological beverage. The release efficiency of active ingredients in the lotus leaves can be remarkably improved through composite strain synergistic fermentation and an ultrasonic-assisted technology, so that the overall efficacy of the product is improved; meanwhile, through compounding of multiple raw materials and addition of natural components, the bitter taste of the lotus leaves is successfully improved, the unique flower and fruit fragrance is formed, and the palatability of the product is improved.

The present invention relates to a method of preventing or treating a fibrotic condition, comprising administering an effective amount of composition to a subject in need thereof; wherein the composition comprises triterpenes extracted from Antrodia-camphorate or Anisomeles indica.

The application provides application of a tremella fuciformis polysaccharide in preparation of an anti-liver cancer drug, and relates to the technical field of biological medicine.The tremella fuciformis polysaccharide in the application can regulate differentiation of monocytes into macrophages, promote classical activation of the macrophages through cascade amplification, and secrete proinflammatory factors, so that the proliferation of liver cancer cells is inhibited, and the effect of resisting liver cancer is achieved.In addition, the tremella fuciformis polysaccharide in the application has good safety, no toxic side effects, can guarantee that the tremella fuciformis polysaccharide is used for preparing a drug for treating liver cancer, is easy to produce, is low in cost, has remarkable effect, has great market development value and good application prospect.

The invention discloses a method for directionally converting a precursor substance in an antrodia camphorata crude extract into high-value triterpenoid antroquinonol in vitro by using recombinant yeast methylated transferase COQ5 and a mutant of the recombinant yeast methylated transferase COQ5. The method comprises the following steps: taking solid-state fermented or wild sporocarp as a raw material, and performing ethanol extraction to obtain a crude extract; in an oleic acid-adenosylmethionine-phosphoric acid buffer system, a wild type or mutant type crude enzyme solution is added, and a reaction is performed at 37 DEG C for 5-12 h, so that the content of antroquinonol can be increased by 2.0-2.6 times. Compared with a PBS blank group, the relative content of antroquinonol in the optimal mutant COQ5-V2 (H132D) catalytic group reaches 260%, and the reaction conditions are mild. The method is simple in process, low in cost and easy to amplify, and the technical bottlenecks that wild antrodia camphorata resources are deficient and the content of active ingredients is low can be solved.

This invention belongs to the field of Antrodia camphorata cultivation technology, specifically relating to a method for cultivating Antrodia camphorata that can increase the triterpenoid content, comprising the following steps: S1. Inoculating the Antrodia camphorata strain into a liquid fermentation medium for initial liquid fermentation culture to obtain initial mycelium; S2. Transferring the initial mycelium to a Cinnamomum camphora culture medium for growth culture to obtain Antrodia camphorata; during the liquid fermentation culture process, periodic ultrasonic treatment is performed, the frequency of the ultrasonic treatment is 35kHz to 60kHz, with a treatment cycle of 2min-3min followed by an interval of 1min-2min, and the treatment is performed for 4h-6h per day. This invention combines liquid fermentation culture with solid culture, which improves production efficiency. Simultaneously, during the liquid fermentation culture stage, periodic ultrasonic treatment stimulates and accelerates the metabolic process of Antrodia camphorata, thereby increasing the content of triterpenoid compounds.

The invention discloses a method for inducing antrodia camphorata mycelia to generate triterpenoid components by utilizing Taiwan cinnamomum kanehirae calluses, and belongs to the technical field of agricultural biology. Taiwan cinnamomum kanehirae cells are obtained through tissue culture, then the Taiwan cinnamomum kanehirae cells and antrodia camphorata strains are co-cultured, and extraction analysis or separation and purification show that the method can induce antrodia camphorata mycelia to generate triterpenoid components. According to the method, the triterpenoid component with various biological effects can be obtained without cultivating the antrodia camphorata sporocarp, and the preparation process is simple and suitable for industrial production.

This invention provides a compound traditional Chinese medicine composition, its preparation method, and its application in the preparation of drugs for treating allergic rhinitis, belonging to the field of traditional Chinese medicine technology. The compound traditional Chinese medicine composition of this invention includes: Antrodia camphorata, Paeonia suffruticosa, Cynanchum paniculatum, Cicadae periostracum, Calculus bovis, Borneol, and Mentha haplocalyx. Antrodia camphorata, the principal ingredient, fundamentally regulates immunity and reduces inflammation and swelling. Paeonia suffruticosa, Cynanchum paniculatum, and Cicadae periostracum assist in anti-allergy and clearing heat and wind. Calculus bovis and Borneol are adjuvant ingredients that resolve phlegm, open the orifices, clear heat, and relieve pain. Mentha haplocalyx guides the medicine upwards and relieves local symptoms. This invention treats allergic rhinitis using a combination of internal and external application. Internal administration exerts a systemic regulatory effect and enhances immunity, while external application to the nose quickly relieves symptoms. The comprehensive formula addresses both the root cause and symptoms, with the various drug components synergistically enhancing anti-inflammatory, anti-allergic, and immunomodulatory effects to achieve the therapeutic effect of treating allergic rhinitis.

The application discloses a characteristic nucleic acid molecule primer, a kit and a method for rapidly and quantitatively detecting Callophoma plicatus. The nucleic acid molecule primer is BSZ2F: 5'-CAAGTGCTCAACCGCTAC-3' and BSZ2R: 5'-TGCTGATTCTCCACGATG-3'. The application adopts a fluorescent quantitative PCR technology to detect, and the detection limit is as low as 0.03 ng / µL. The method is simple, specific, short in time and capable of being completed within 2 hours.

The invention belongs to the technical field of biology, and particularly relates to a preparation method of antrodia camphorata ferment. According to the method, Trichoderma longibrachiatum and Bacillus licheniformis are mixed to prepare a composite fermentation seed solution, the composite fermentation seed solution is used for carrying out composite fermentation on a culture medium composed of cassava residues and wood chips, and the pretreated antrodia camphorata culture medium is prepared. Then, inoculating an antrodia camphorata strain into the culture medium for fermentation, and introducing a heat stress treatment step in the fermentation process; according to the method, synthesis and dissolution of triterpenoids and polysaccharide active ingredients of antrodia camphorata are remarkably promoted through combination of the synergistic effect of specific strains and physical stress, the technical problem that in the prior art, active substances of antrodia camphorata are not sufficiently dissolved out is solved through unique strain combination and process design, and the method has extremely high application value and market prospects.

The invention relates to the technical field of natural products, and particularly discloses a composition, a preparation method of the composition and application of the composition in preparation of a product with a hangover alleviating effect. The composition comprises the following traditional Chinese medicine raw materials: 10-20 parts of hovenia dulcis thunb; 10 to 20 parts of ampelopsis grossedentata; 5-15 parts of radix puerariae; 5 to 15 parts of hawthorn; and 5-10 parts of licorice. The traditional Chinese medicine raw materials also comprise nutmeg, radix sophorae flavescentis, antrodia camphorata and / or flos puerariae; furthermore, the invention also provides another composition, and the composition is obtained by uniformly mixing the traditional Chinese medicine extract with the yeast extract, the corn peptide, the oyster peptide and the L-cysteine, wherein the weight ratio of the traditional Chinese medicine extract to the yeast extract to the corn peptide to the oyster peptide to the L-cysteine is 1: (8-9): (0.3-1): (0.3-1): (0.5-1.5). Researches show that the composition has the effect of dispelling the effects of alcohol; therefore, the compound has an important application value when being used as an active ingredient for preparing medicines or health-care products with the effect of dispelling the effects of alcohol.

The invention provides application of an antrodia camphorata extract. The antrodia camphorata extract has the effect of regulating and controlling a downstream path of signal transmission mediated by Nrf2 protein activation. Therefore, the antrodia camphorata extract can be used for preparing the pharmaceutical composition for regulating and controlling the Nrf2 protein, and has application potential in related markets.

This invention discloses a high-efficiency greenhouse cultivation method for *Antrodia camphorata* in the field of medicinal fungal cultivation technology, including strain activation, substrate preparation, inoculation culture, and fruiting management. The substrate contains wheat bran, corn flour, sucrose, potassium dihydrogen phosphate, magnesium sulfate, zinc sulfate, and lanthanum-modified multi-level carbon-silicon composite nanomaterials, with vitamin B1 added after sterile filtration. After static cultivation in a greenhouse, the mycelium is induced to produce fruiting bodies through atomized spraying, and then dried after harvesting. The lanthanum-modified multi-level carbon-silicon composite nanomaterials are prepared by chitosan coordinating with lanthanum nitrate, precipitating with anhydrous ethanol, assembling with mesoporous silica, cross-linking with 3-aminopropyltriethoxysilane and tetraethyl orthosilicate under glacial acetic acid catalysis, and finally activated by gradient calcination and washing with anhydrous ethanol. This invention avoids the Maillard reaction and surface crusting during high-temperature sterilization, protects the activity of the lanthanum oxide crystal phase, significantly improves the mycelial growth rate and triterpenoid yield of *Antrodia camphorata*, and shortens the cultivation cycle.