172 results about "Antrodia cinnamomea" patented technology

The invention provides an antrodia camphorate cultivation method; firstly, pre-cultivating the antrodia camphorate on a slant culture medium, taking out a part of pre-cultivated antrodia camphorate to inoculate a liquid culture medium, and then inoculating the antrodia camphorate cultivated in the liquid culture medium to a solid culture medium for the cultivation. The antrodia camphorate cultivated in the method has similar effects of wild antrodia camphorate, including effects of restraining growth activity of human cancer cells and cancer auxiliary treatment, also having abilities of restraining human low density lipoprotein oxidation for lowering incidence rates of artery hardening and restoring damaged liver functions.

The invention provides a camphor tree sesame fruiting body cultivation method which comprises the following steps: culture media containing leaven are taken for fermentation for 3 to 30 days under the temperature of 5 to 35 DEG C; saw dusts are added into the culture media after fermentation and stirred; the saw dusts and the culture media are arranged inside a vessel; the vessel provided with the saw dusts and the culture media is sterilized; camphor tree sesame spawns are taken to be inoculated into the vessel containing the saw dusts and the culture media and cultivated into mycelium under the temperature of 5 to 35 DEG C; saw dusts containing the camphor tree sesame mycelium are inoculated to a bed wood; the bed wood which has been inoculated with the spawns is cultivated under the temperature of 5 to 35 DEG C and in the humidity of 65 to 85 percents; after residual saw dusts on the bed wood are removed, the bed wood is cultivated under the temperature of 15 to 35 DEG C and in the humidity of 80 to 98 percents for a period of time, and a camphor tree sesame fruiting body can be formed. The invention also provides a cultivation method for camphor tree sesame mycelium and the camphor tree sesame fruiting body obtained by cultivation according to the method. The camphor tree sesame fruiting body cultivated by the invention has the characteristics of massiness of the fruiting body and content of triterpene close to wild camphor tree sesame.

The invention discloses an antrodia cinnamomea submerged fermentation compound product and a preparation method thereof. The preparation method comprises the following steps: preparing a submerged fermentation medium from poria cocos powder, Chinese yam powder, radix puerariae powder, bee pollen, soybean meal, yeast powder, glucose, dipotassium phosphate, magnesium sulfate and the like; carrying out submerged fermentation and cultivation on an antrodia cinnamomea mycelium fermentation liquid rich in antrodia cinnamomea polysaccharide; and carrying out supercritical CO2 technical extraction, enzymolysis, separation, filtration and concentration to prepare Chinese herbal medicine extraction liquids such as fingered citrons, sealwort, wolfberries, fructus amomi, emblic leafflower fruits, turnjujube and the like. According to the invention, the content of active components such as antrodia cinnamomea polysaccharide and the like is optimized and the preparation method is high in yield, short in time and low in cost, can be used for industrial production, is an efficient and environment-friendly bioengineering production technology and has remarkable development and application prospects.

The invention discloses an antrodia cinnamomea growth medium and a preparation method and application thereof, belonging to the field of bioengineering technology. The antrodia cinnamomea growth medium consists of the following raw materials: 100-250ml of 2% boiled liquid of cinnamomum camphora leaves, 150-250g of potato, 12-18g of glucose, 1-5g of sodium chloride, 0.005-0.03g of cinnamomum camphora oil and water with the rest volume of 1,000ml; the pH is 6.5-7.0. Compared with the prior art, the antrodia cinnamomea growth medium disclosed by the invention has the beneficial effects that (1) the medium is simple to prepare and convenient to store, the adopted technology is mature, and the medium contains all nutritional ingredients required for the antrodia cinnamomea growth and is suitable for the culture and large-scale industrial fermentation production of antrodia cinnamomea; (2) the cultured antrodia cinnamomea mycelia have high biomass and high intracellular triterpene output, wherein the biomass of mycelia reaches 12.1-14.2g/1,000ml, and the intracellular triterpene output reaches 33.32-46.54mg/g.

The invention discloses an anti-inflammatory effective component extract of antrodia cinnamomea sporocarp, a preparation method and application thereof. The preparation method comprises the following steps of: drying the antrodia cinnamomea sporocarp; and extracting the dried antrodia cinnamomea sporocarp by using ethanol, and collecting an ethanol extract to obtain the anti-inflammatory effective component extract of the antrodia cinnamomea sporocarp. In order to achieve the better extraction effect, the method comprises the following steps of: concentrating the ethanol extract further; layering a concentration product by using water and ethyl acetate; and collecting an ethyl acetate extract to obtain the anti-inflammatory effective component extract. The anti-inflammatory effective component extract of the antrodia cinnamomea sporocarp and Antrocamphin A separated from the antrodia cinnamomea sporocarp can reduce the expression of nitric oxide synthase or cycloxygenase 2 to inhibit pro-inflammation molecules from generating.

In this patent, we isolated a novel compound from fruiting body of Antrodia cinnamomea, namely, (22R)-5α-lanosta-8,24-dien-3β,15α,21-triol. This compound possesses preferential cytotoxicity against human leukemia, pancreatic cancer, esophageal cancer, hepatoma, and cervical cancer cells.

The invention relates to a preparation method of health wine with antrodia cinnamomea as a main raw material. The preparation method comprises the following steps of drying, grinding and sieving antrodia cinnamomea and then adding white wine with alcohol content of 75 to soak and sterilize antrodia cinnamomea for 2-3 days, stirring antrodia cinnamomea, and removing the dirt; removing the residues through sieving, collecting antrodia cinnamomea after sterilization and dirt removal and a soak solution in a container, then adding white wine with alcohol content of 40-50, sealing the container away from light and soaking antrodia cinnamomea for 50-60 days, filtering the wine to remove the residues, and collecting the supernatant; adding L-arabinose to the supernatant to prepare a solution, and sealing the solution and standing for 20 days away from light, thus obtaining the antrodia cinnamomea health wine. The preparation method has the beneficial effects that the preparation method is simple and practicable and can be used for household or industrial production; the obtained antrodia cinnamomea health wine has high cleanliness, is rich in active substances such as triterpenoids, beta-glucan, adenosine, superoxide dismutase, amino acid and the like, and has the functions of resisting cancers and inflammation, protecting livers, regulating immunities, reducing blood pressure and blood lipid, maintaining beauty and keeping young.

A solid medium of Antrodia cinnamomea comprises 20 to 50 wt % soybean; 30 to 50 wt % coixseed; and 20 to 50 wt % sorghum. A culturing method of Antrodia cinnamomea, comprises providing a solid medium comprising soybean, coixseed and sorghum; preparing a solution of Antrodia cinnamomea, inoculating the solution of Antrodia cinnamomea on the solid medium in a concentration of 1×10³ to 1×10⁶ cfu on per 100 g of solid medium, and statically culturing the solution of Antrodia cinnamomea on the solid medium till obtaining hyphae adhered on the solid medium; and culturing the solution of Antrodia cinnamomea by intermittent shaking in a frequency of every 5 to 7 days shaking 5 to 10 minutes, with a period of the culturing by intermittent shaking being more than 40 days.

The present invention relates to a method for inhibiting tumor growth, in particular to the method using dehydrosulphurenic acid to inhibit the growth of leukemia cell or pancreatic cancer cell by a compound extracted and purified from Antrodia cinnamomea. Dehydrosulphurenic acid of the invention can be used as a pharmaceutical composition to inhibit the tumor growth of leukemia or pancreatic cancer.

The invention discloses a compound isolated from Antrodia camphorate and shown as formula I. Specifically, R1 is hydrogen or acetyl. The invention further discloses application of the compound to preparation of drugs for inhibiting tumor growth, wherein the tumor can be one of lung adenocarcinoma, colon cancer, prostate cancer and liver cancer.

A method of culturing Antrodia cinnamomea with high Triterpenoids includes mixing a malt extract, glucose, peptone, agar, and a mushroom extract with distilled water to obtain a culture medium; providing the culture medium to a high-pressure sterilization machine for sterilization to obtain a sterilized culture medium; providing the sterilized culture medium to an incubator in a disinfection environment to obtain a solidified culture medium after a predetermined time; transplanting Antrodia cinnamomeas strains to the solidified culture medium at separated positions; and exposing the solidified culture medium under a beam.

Antrodia cinnamomea is a valuable medical fungi. At present, artificial cultivation method of antrodia cinnamomea comprise cinnamomum kanehirai linden cultivation method, solid cultivation method and liquid fermentation method. However, the cinnamomum kanehirai linden cultivation method is long in cultivating period, high in cost, but low in antrodia cinnamomea output; and the antrodia cinnamomea obtained by the solid cultivation method and liquid fermentation method is few in nutrient content. The invention provides a Chinese fir board cultivating method of antrodia cinnamomea; the method comprises the following steps: using Chinese fir boards in 5*10*25 centimeters as carriers, soaking and high-temperature sterilizing, then inoculating antrodia cinnamomea strain for cultivation; growing antrodia cinnamomea fruit bodies in association with nutrition powders on the Chinese fir boards; the obtained antrodia cinnamomea fruit bodies are high in output and short in growth period. The cultivation technology of cultivating antrodia cinnamomea without cinnamomum kanehirai is achieved, thus not only being beneficial to protecting the rare cinnamomum kanehirai resources, but also improving the development of traditional Chinese medicine enterprises of antrodia cinnamomea raw materials, thereby achieving good ecological effect, economic effect and social effect.

A method for cultivating antrodia cinnamomea includes transplanting bacterial strains of antrodia cinnamomea into a petri dish receiving culture ingredients. The bacterial strains of antrodia cinnamomea are transferred into a culture container after mycelia of the bacterial strains of antrodia cinnamomea have grown to a predetermined area. The culture container receives a culture fluid. A formula composition of a culture medium for cultivating antrodia cinnamomea is added into the culture container after the mycelia of the bacterial strains of antrodia cinnamomea have grown in the culture container. The formula composition of the culture medium for cultivating antrodia cinnamomea includes growth basal ingredients and cultivating basal ingredients. The growth basal ingredients includes hydroxyhopanone. Mycelia of antrodia cinnamomea fully use hydroxyhopanone precursors of agarwood during a growth procedure of the mycelia of antrodia cinnamomea, such that the mycelia of antrodia cinnamomea have a higher percentage of triterpenoids after fermentation.

Disclosed is a compound isolated from Antrodia camphorata, represented by formula I:

wherein R1 is a hydrogen atom or an acetyl group; and a method of inhibiting cancer cell growth by using the compound, the cancer is selected from the group consisting of lung cancer, colon cancer, prostate cancer, liver cancer and breast cancer.

Provided is a method for preparing a culture medium for Antrodia cinnamomea liquid fermentation. The culture medium comprises white granulated sugar, enzyme liquid filter pulp, soyabean protein powder, monopotassium phosphate, magnesium sulfate, vitamin B1 and agar, or comprises brown sugar, the enzyme liquid filter pulp, enzyme powder residues, corn steep liquor, the monopotassium phosphate, the magnesium sulfate, the vitamin B1 and the agar.

The invention relates to a medicament for treating liver cancer, which is prepared from 19 Chinese medicinal herbs including glossy ganoderma, antrodia cinnamomea, elecampane inula root, emblic leafflower fruit, meconopsis, giant cardiocrinum, rose mallow root, tabasheer, herpetospermum caudigerum wall, phymatopsis hastate, blumea leaf or twig, fructus phyllanthi, long pepper, dracocephalum tanguticum, liquoric root, cassia bark, areca seed, Chinese wolfberry fruit and litchee bark. The medicament is a pure natural Chinese medicine, does not have toxic or side effect and have injury to human bodies, has the effects of tonifying spleen and regulating flow of vital energy, cooling and refreshing and detoxicating and expelling toxic heat and polluted toxic qi of internal organs, and is mainly used for treating primary liver cancer. By long-term administration, the pathological changes of cells can be inhibited and prevented, appetite is improved obviously, and the immunity is enhanced.

The invention relates to a method for preparing 2-phenethyl alcohol by adopting an antrodia cinnamomea submerged fermentation technology. The method comprises the following steps: (1) strain activation; (2) seed liquid preparation; 3) submerged fermentation cultivation of liquid; (4) solid-liquid separation and extraction of the fermentation liquor. The method is simple and convenient in production technology, and has a broad industrial development prospect; a fermentation culture solution is separated into mycelium and the fermentation liquor after being filtered, 2-phenethyl alcohol is obtained after the fermentation liquor is extracted, and the fermented mycelium can be applied to other biological researches; the method has important significance on effective utilization of agricultural by-products and wastes, improvement of additional value of edible and medicinal fungi industries, and comprehensive utilization of agriculture and forestry resources.

The invention is applicable to the field of Antrodia cinnamomea cultivation and provides a method of culturing Antrodia cinnamomea by dish. The method comprises following steps: a step of culture medium preparing: weighing following components according to the mass volume ratio of water: 5 to 40 g/L of wheat bran, 5 to 40 g/L of corn starch, 5 to 40 g/L of agar, 1 to 3 g/L of peptone, 0.5 to 4.0 g/L of magnesium sulfate and 0.5 to 10ml/L of olive oil to prepare a dish culture medium for Antrodia cinnamomea; a step of inoculating: under bacteria-free environment, Antrodia cinnamomea strains are inoculated on the culture medium; a step of cultivating: the inoculated culture medium is placed in a environment with constant temperature and constant humidity for 3-12 months. The method of culturing Antrodia cinnamomea by dish comprises a step of culture medium preparing, a step of inoculating and a step of cultivating, wherein the culture medium is a dish type medium. Through formula improvement and cultivation step optimization, the triterpenoid content in obtained Antrodia cinnamomea is substantially increased.

The invention relates to the field of health food and discloses a preparation method of antrodia cinnamomea health wine. The method comprises the following steps of drying and grinding antrodia cinnamomea and then filling ground antrodia cinnamomea in a chromatographic column, adding white wine and carrying out percolation, preparing a solution from percolate and L-arabinose, and sealing the solution and standing away from light, thus obtaining the antrodia cinnamomea health wine. The preparation method has the beneficial effects that the adopted equipment is simple and cheap; the process conditions are mild and are easy to control; the raw materials have high use ratios and extraction efficiency and have great commercial values; the obtained antrodia cinnamomea health wine is rich in active ingredients such as triterpenoids, beta-glucan, adenosine, superoxide dismutase, amino acid and the like, is abundant in variety and high in content, and has various physiological functions; meanwhile, the method has the effect of effectively removing harmful ingredients, and the product is clean and safe and has excellent quality.

The invention relates to an antrodia camphorate composition. The composition is prepared from the following components in parts by weight: 100-135 parts of antrodia camphorate mycelia, 12-18 parts of coenzyme Q10, 15-30 parts of gynostemma pentaphylla, 20-35 parts of pseudo-ginseng, 5-12 parts of American ginseng, 3-6 parts of wolfberry fruits and 20-40 parts of olive extract. The antrodia camphorate composition can be used for lowering the contents of total blood serum cholesterol, low-density lipoprotein cholesterol and triglyceride and improving the content of high-density lipoprotein cholesterol; the liver damage can be reduced and the liver function can be improved; and the antrodia camphorate composition has the effects of activating blood and removing blood stasis, lowering blood pressure and preventing cardiovascular and cerebrovascular events, and also has the effects of lowering blood glucose, improving the immunity and resisting tumors.