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Method of culturing Antrodia cinnamomea by dish

A culturing method and a turmeric technique, applied in the field of Antrodia camphorata culturing, can solve the problems of long culturing time, less basswood resources and high cost, and achieve the effects of shortening the maturation period, optimizing the inoculation process, and increasing the content.

Inactive Publication Date: 2016-12-14
SHENZHEN RENTAI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Basswood cultivation method is currently the closest cultivation method for Antrodia cinnamomea and wild Antrodia cinnamomea triterpenoids, but it also has certain defects, such as: long cultivation time, high cost, and less basswood resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036](1) Culture medium preparation steps: weigh bran 20g / L, add water, boil for 40min, filter and take the filtrate, then add cornstarch 20g / L into the filtrate of bran, heat and gelatinize after stirring evenly, after gelatinization is completed Add agar 20g / L, peptone 1.5g / L, magnesium sulfate 2.0g / L olive oil 2ml / L in turn, stir to dissolve completely, and replenish water to 1L. Sterilize by high temperature and high pressure in a sterilizing pot for 20 minutes, pour it into a petri dish while it is hot, with a thickness of about 2 cm, and let it cool to solidify.

[0037] (2) Strain preparation: liquid seed medium composition: glucose: 20.0g / L, malt extract 20.0g / L, peptone 1.0g / L, magnesium sulfate 0.5g / L, pH 4.0, distilled water to volume, high-pressure steam Sterilize for 20 minutes. Aseptically collect the spores of Antrodia camphorata, put them into shake flasks for liquid culture, the culture conditions are temperature 28°C, rotation speed 160rpm, and culture for ...

Embodiment 2

[0042] (1) Culture medium preparation steps: weigh bran 10g / L, add water, boil for 40min, filter and take the filtrate, then add cornstarch 10g / L into the filtrate of bran, heat and gelatinize after stirring evenly, after gelatinization is completed Add agar 15g / L, peptone 1.0g / L, magnesium sulfate 1.0g / L olive oil 1ml / L, stir to dissolve completely, and replenish water to 1L. Sterilize by high temperature and high pressure in a sterilizing pot for 20 minutes, pour it into a petri dish while it is hot, with a thickness of about 2 cm, and let it cool to solidify.

[0043] (2) Strain preparation: liquid seed medium composition: glucose: 20.0g / L, malt extract 20.0g / L, peptone 1.0g / L, magnesium sulfate 0.5g / L, pH 4.0, distilled water to volume, high-pressure steam Sterilize for 20 minutes. Aseptically collect the spores of Antrodia camphorata, put them into shake flasks for liquid culture, the culture conditions are temperature 28°C, rotation speed 160rpm, and culture for 15 days...

Embodiment 3

[0048] (1) Culture medium preparation steps: weigh bran 20g / L, add water, boil for 40min, filter and take the filtrate, then add cornstarch 20g / L into the filtrate of bran, heat and gelatinize after stirring evenly, after gelatinization is completed Add agar 20g / L, peptone 1.5g / L, magnesium sulfate 2.0g / L olive oil 2ml / L in turn, stir to dissolve completely, and replenish water to 1L. Sterilize by high temperature and high pressure in a sterilizing pot for 20 minutes, pour it into a petri dish while it is hot, with a thickness of about 2 cm, and let it cool to solidify.

[0049] (2) Strain preparation: liquid seed medium composition: glucose: 20.0g / L, malt extract 20.0g / L, peptone 1.0g / L, magnesium sulfate 0.5g / L, pH 4.0, distilled water to volume, high-pressure steam Sterilize for 20 minutes. Aseptically collect the spores of Antrodia camphorata, put them into shake flasks for liquid culture, the culture conditions are temperature 28°C, rotation speed 160rpm, and culture for...

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Abstract

The invention is applicable to the field of Antrodia cinnamomea cultivation and provides a method of culturing Antrodia cinnamomea by dish. The method comprises following steps: a step of culture medium preparing: weighing following components according to the mass volume ratio of water: 5 to 40 g / L of wheat bran, 5 to 40 g / L of corn starch, 5 to 40 g / L of agar, 1 to 3 g / L of peptone, 0.5 to 4.0 g / L of magnesium sulfate and 0.5 to 10ml / L of olive oil to prepare a dish culture medium for Antrodia cinnamomea; a step of inoculating: under bacteria-free environment, Antrodia cinnamomea strains are inoculated on the culture medium; a step of cultivating: the inoculated culture medium is placed in a environment with constant temperature and constant humidity for 3-12 months. The method of culturing Antrodia cinnamomea by dish comprises a step of culture medium preparing, a step of inoculating and a step of cultivating, wherein the culture medium is a dish type medium. Through formula improvement and cultivation step optimization, the triterpenoid content in obtained Antrodia cinnamomea is substantially increased.

Description

technical field [0001] The invention belongs to the field of Antrodia camphorata cultivation, and in particular relates to a dish-type culture method of Antrodia camphorata. Background technique [0002] Antrodia camphorata (Antrodia camphorata), also known as cinnamomum cinnamomea, belongs to Basidiomycetes, Polyporaceae, Thinporus, and perennial fungi. Forest Ruby. Antrodia Antrodia contains a variety of physiologically active ingredients, such as polysaccharides, triterpenoids, superoxide dismutase (SOD) adenosine, proteins (including immune proteins), multivitamins, trace elements, nucleic acids, lectins, amino acids, cholesterol, Lignin, blood pressure stabilizing substances, etc. Its physiologically active functions include anti-tumor, enhancing immunity, anti-virus, anti-allergic, anti-hypertensive, inhibiting platelet aggregation, lowering blood pressure, lowering cholesterol, anti-bacterial, protecting the liver, etc. Among them, triterpenoids are medicinal fungi ...

Claims

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Application Information

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IPC IPC(8): A01G1/04
CPCA01G18/00
Inventor 赵宗杰张向阳方晓柴行刘家飞张康
Owner SHENZHEN RENTAI BIOLOGICAL TECH
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