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Whitening and repairing mask containing probiotic active extracting solution and nicotinamide

A technology of extract and nicotinamide, which is applied in the field of cosmetic formula, can solve the problems of not outstanding whitening effect and low tyrosinase inhibition rate, and achieve the effect of no cytotoxicity, broad market potential, and good moisturizing effect

Active Publication Date: 2020-08-18
宁波格鲁康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Niacinamide products in the prior art can only inhibit the hydrolysis of nicotinamide by avoiding light, pH control, and low temperature
In addition, the tyrosinase inhibition rate of nicotinamide itself is very low, and the whitening effect is not outstanding

Method used

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  • Whitening and repairing mask containing probiotic active extracting solution and nicotinamide
  • Whitening and repairing mask containing probiotic active extracting solution and nicotinamide
  • Whitening and repairing mask containing probiotic active extracting solution and nicotinamide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation method of the fermentation activity extract of bacillus subtilis nk1 is as follows:

[0037] (1) Strain activation:

[0038] The medium used for activation is milk medium, and the formula refers to the existing technology, and the formula is as follows: 500g fresh milk, 50g sucrose, configured into 1L, and sterilized at high temperature;

[0039] The activation conditions are: 37°C shaker culture for 12 hours, the amount of bacteria after activation is 2.2×10 8 CFU / mL of seed solution.

[0040] (2) Fermentation culture:

[0041] The formula of the special liquid medium used for fermentation is as follows: 20g of glucose, 10g of peptone, 10g of yeast extract, 8g of soybean powder, 3g of nicotinamide, 3g of Antrodia camphorata extract, 3mL of Tween-80, 0.6g of MgSO 4 ·7H 2 O, 0.3g of MnSO 4 4H 2 O, 2g of K 2 HPO 4 Mix with 1000mL of distilled water, adjust the pH value to 7.0 and obtain a sterile medium after autoclaving;

[0042] Inoculate the se...

Embodiment 2

[0044] Embodiment 2 comparative example

Embodiment 3

[0050] Cytotoxicity test:

[0051] The fermentation activity extract of Bacillus subtilis nk1 obtained in Example 1 was dissolved in serum-free DMEM culture fluid, and 4 samples were prepared, with mass fractions of 0.25%, 0.5%, 1%, and 2%, respectively, and serum-free DMEM culture fluid The negative control sample group was used for MTT detection to evaluate the cytotoxicity of the fermentation activity extract of Bacillus subtilis nk1.

[0052] Inoculate L929 cells into a 96-well plate at 100 μL / well, and adjust the concentration to 1×10 5 cells / mL, at 37°C, 5% CO 2 After culturing for 24 hours in serum-free DMEM culture solution for adherence, the culture solution was discarded, and 100 μL of sample solutions with different mass fractions were added to each well. The negative control group continued to culture in serum-free DMEM for 48 hours. Add 20 μL of 5 mg / mL MTT solution and incubate for 4 h in an incubator protected from light. Discard the supernatant and add 100 μ...

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Abstract

The invention relates to a whitening and repairing mask using a bacillus subtilis nk1 fermentation active extracting solution as a main functional component. An antrodia cinnamomea extract is added into a bacillus subtilis nk1 culture medium, so that the whitening effect can be further enhanced, the repairing activity of the antrodia cinnamomea extract is greatly improved, and the antrodia cinnamomea extract and nicotinamide have the effect of synergistically improving the effect of a bacillus subtilis nk1 fermentation supernatant.

Description

technical field [0001] The invention relates to the technical field of cosmetic formulations, in particular to a whitening and repairing facial mask containing probiotic active extracts and nicotinamide. Background technique [0002] Facial mask is a facial skin cosmetic that integrates cleaning, care and nutrition, and is deeply loved by female consumers. By blocking the contact between the skin and the air, it inhibits sebum secretion and sweat evaporation to increase the temperature of the skin, thereby promoting the blood circulation of the facial skin; it can also soften cuticles, expand pores, and play a role in cleaning and maintaining the skin. At the same time, the nutrients and functional ingredients contained in the mask can penetrate into the skin, enhance the function of the skin, and effectively improve the skin quality. [0003] Niacinamide is a water-soluble vitamin and a member of the vitamin B family. As a basic vitamin supplement in clinical dermatology ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/9728A61K8/67A61Q19/02A61Q19/00
CPCA61K8/675A61K8/9728A61K8/99A61Q19/00A61Q19/02
Inventor 罗春梅
Owner 宁波格鲁康生物科技有限公司
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