Anti-inflammatory effective component extract of antrodia cinnamomea sporocarp, preparation method and application thereof
A technology of effective parts and Antrodia camphorata, applied in the field of effective parts of Antrodia camphorata fruiting bodies, can solve problems to be further understood and the like
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Embodiment 1
[0049] Example 1 Preparation of Anti-inflammatory Effective Fracture Extract of Antrodia Camphorata Fruiting Bodies
[0050] First, the fruiting body of Antrodia camphorata is dried by freeze-drying, and then 580 grams of the above-mentioned freeze-dried fruiting body of Antrodia camphorata is extracted with 95% ethanol at 42-45 ° C to obtain the ethanol extract (ACE) of the present invention That is, the extract from the anti-inflammatory effective part of the fruiting body of Antrodia camphorata. The aforementioned drying method can be any drying method known in the art without limitation.
[0051] Then the aforementioned ethanol extract was concentrated under vacuum (rotary evaporator) to obtain 183.9 g of concentrated product. The resulting concentrated product is then divided into an ethyl acetate layer and a water-soluble layer with ethyl acetate and water, wherein the water-soluble layer is marked as the water-soluble fraction (ACE-water), and the ethyl acetate layer i...
Embodiment 2
[0052] Example 2 Ethanol extract (ACE), ethyl acetate extract (ACE-EA) and water-soluble fraction (ACE-water) in the activity test of inhibiting nitric oxide: macrophage cell line RAW 267.4
[0053] In this embodiment, the ethanol extract (ACE), ethyl acetate extract (ACE-EA) and water-soluble fraction (ACE-water ) in the activity test for inhibiting nitric oxide.
[0054] First, the RAW 267.4 cell line was cultured according to the cell culture method described in Example 1, and then divided into three groups, experimental group 1 (ethanol extract, ACE), experimental group 2 (ethyl acetate extract, ACE-EA) And experimental group three (water-soluble fraction, ACE-water). For each group of cells, the aforementioned ethanol extract, the aforementioned ethyl acetate extract, and the aforementioned water-soluble fraction were added to the culture medium at different concentrations (1, 10, 25, and 50 μg / ml), and then lipopolysaccharide (LPS ) was added to the culture solution to...
Embodiment 3
[0059] Example 3 Activity Test of Ethanol Extract (ACE) in Inhibiting Nitric Oxide: In Vivo Experiment of Mouse Model
[0060] In this example, mice were used as model organisms to carry out the activity of the ethanol extract (ACE) prepared in Example 1 on inhibiting nitric oxide. The mice used were prepared according to the aforementioned experimental mice section.
[0061] [experimental design]
[0062] First, every six mice were divided into one group, and divided into six groups in total. According to the configuration in Table 2 below, before injecting lipopolysaccharide (5 μg / kg), the mice in each group were given intraperitoneal injection (intraperitoneal injection). Antrodia camphorata ethanol extract (ACE, 100, 300, 500 mg / kg), curcumin (curcumin) prepared in Example 1 of different concentrations or not given injection, wherein the control group only injected dimethyl sulfoxide (DMSO) alone . Wherein, curcumin (curcumin) is a known anti-inflammatory agent, which i...
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