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Anti-inflammatory effective component extract of antrodia cinnamomea sporocarp, preparation method and application thereof

A technology of effective parts and Antrodia camphorata, applied in the field of effective parts of Antrodia camphorata fruiting bodies, can solve problems to be further understood and the like

Inactive Publication Date: 2011-08-10
NATIONAL CHUNG HSING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been confirmed in many scientific studies that the methanol extract and hot water extract of Antrodia camphorata mycelium, the methanol extract, ethanol extract and ethyl acetate extract of fruiting bodies have excellent anticancer activity, and some studies It is pointed out that the mycelia and fruiting bodies of Antrodia camphorata contain some triterpenoid compounds, which may have potential anti-inflammatory properties. To be further understood

Method used

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  • Anti-inflammatory effective component extract of antrodia cinnamomea sporocarp, preparation method and application thereof
  • Anti-inflammatory effective component extract of antrodia cinnamomea sporocarp, preparation method and application thereof
  • Anti-inflammatory effective component extract of antrodia cinnamomea sporocarp, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Preparation of Anti-inflammatory Effective Fracture Extract of Antrodia Camphorata Fruiting Bodies

[0050] First, the fruiting body of Antrodia camphorata is dried by freeze-drying, and then 580 grams of the above-mentioned freeze-dried fruiting body of Antrodia camphorata is extracted with 95% ethanol at 42-45 ° C to obtain the ethanol extract (ACE) of the present invention That is, the extract from the anti-inflammatory effective part of the fruiting body of Antrodia camphorata. The aforementioned drying method can be any drying method known in the art without limitation.

[0051] Then the aforementioned ethanol extract was concentrated under vacuum (rotary evaporator) to obtain 183.9 g of concentrated product. The resulting concentrated product is then divided into an ethyl acetate layer and a water-soluble layer with ethyl acetate and water, wherein the water-soluble layer is marked as the water-soluble fraction (ACE-water), and the ethyl acetate layer i...

Embodiment 2

[0052] Example 2 Ethanol extract (ACE), ethyl acetate extract (ACE-EA) and water-soluble fraction (ACE-water) in the activity test of inhibiting nitric oxide: macrophage cell line RAW 267.4

[0053] In this embodiment, the ethanol extract (ACE), ethyl acetate extract (ACE-EA) and water-soluble fraction (ACE-water ) in the activity test for inhibiting nitric oxide.

[0054] First, the RAW 267.4 cell line was cultured according to the cell culture method described in Example 1, and then divided into three groups, experimental group 1 (ethanol extract, ACE), experimental group 2 (ethyl acetate extract, ACE-EA) And experimental group three (water-soluble fraction, ACE-water). For each group of cells, the aforementioned ethanol extract, the aforementioned ethyl acetate extract, and the aforementioned water-soluble fraction were added to the culture medium at different concentrations (1, 10, 25, and 50 μg / ml), and then lipopolysaccharide (LPS ) was added to the culture solution to...

Embodiment 3

[0059] Example 3 Activity Test of Ethanol Extract (ACE) in Inhibiting Nitric Oxide: In Vivo Experiment of Mouse Model

[0060] In this example, mice were used as model organisms to carry out the activity of the ethanol extract (ACE) prepared in Example 1 on inhibiting nitric oxide. The mice used were prepared according to the aforementioned experimental mice section.

[0061] [experimental design]

[0062] First, every six mice were divided into one group, and divided into six groups in total. According to the configuration in Table 2 below, before injecting lipopolysaccharide (5 μg / kg), the mice in each group were given intraperitoneal injection (intraperitoneal injection). Antrodia camphorata ethanol extract (ACE, 100, 300, 500 mg / kg), curcumin (curcumin) prepared in Example 1 of different concentrations or not given injection, wherein the control group only injected dimethyl sulfoxide (DMSO) alone . Wherein, curcumin (curcumin) is a known anti-inflammatory agent, which i...

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Abstract

The invention discloses an anti-inflammatory effective component extract of antrodia cinnamomea sporocarp, a preparation method and application thereof. The preparation method comprises the following steps of: drying the antrodia cinnamomea sporocarp; and extracting the dried antrodia cinnamomea sporocarp by using ethanol, and collecting an ethanol extract to obtain the anti-inflammatory effective component extract of the antrodia cinnamomea sporocarp. In order to achieve the better extraction effect, the method comprises the following steps of: concentrating the ethanol extract further; layering a concentration product by using water and ethyl acetate; and collecting an ethyl acetate extract to obtain the anti-inflammatory effective component extract. The anti-inflammatory effective component extract of the antrodia cinnamomea sporocarp and Antrocamphin A separated from the antrodia cinnamomea sporocarp can reduce the expression of nitric oxide synthase or cycloxygenase 2 to inhibit pro-inflammation molecules from generating.

Description

technical field [0001] The present invention relates to the effective part of the fruiting body of Antrodia camphorata, in particular to an extract of the effective part of the fruiting body of Antrodia camphorata and a preparation method thereof, and also relates to the use of the extract of the effective part of the fruiting body of Antrodia camphorata in the preparation of anti-inflammatory drugs , belonging to the field of effective parts of antrodia camphorata fruiting bodies. Background technique [0002] Inflammation is a major feature in many pathological states. Taking bacterial infection as an example, lipopolysaccharide (LPS, also known as endotoxin) located on the surface of bacteria will activate macrophages in the host. Under normal conditions , This activation process will help the activation of the host's overall immune system to achieve the purpose of eliminating pathogenic bacteria. However, in some abnormal physiological reactions (which may be accompanie...

Claims

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Application Information

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IPC IPC(8): A61K36/07A61K31/09A61P29/00
Inventor 王升阳
Owner NATIONAL CHUNG HSING UNIVERSITY
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