33 results about "Sterane" patented technology

Disclosed are the isolation, purification and analysis of the triterpenoid compositions (including ergostane and lanostane) in the fruiting body of Antrodia cinnamomea using HPLC and NMR, as well as the stereo structures and the amounts of the triterpenoid compositions. The cytotoxicity of triterpenoids is also revealed. Based on the aforementioned techniques, the presence and amounts of ergostane and lanostane in the drugs, healthcare food or other goods are able to be detected.

The invention provides a method utilizing C29 sterane isomerization parameters for representing oil-gas migration. The method includes: firstly, creating a base curve according to thermal evolution of source rocks and crude oil; secondly, subjecting oil samples to chromatography / mass spectrometry analytical test; thirdly, calculating the C29 sterane isomerization parameters of the oil samples, tracing oil-gas migration paths according to a mode utilizing the C29 sterane isomerization parameters for representing oil-gas migration, and predicting favorable oil-gas accumulation areas. The method utilizing the C29 sterane isomerization parameters for representing oil-gas migration has the advantages that by means of calculating the C29 sterane isomerization parameters of the crude oil in subsurface reservoirs and analyzing change trends of the subsurface reservoirs, an oil-gas migration process including migration paths, power conditions, migration distances and the like is traced effectively, so that oil-gas accumulation study can be detailed, and the method is of important significance to an oil-gas migration mechanism and distribution regularities.

The invention provides a comprehensive two-dimensional gas chromatography quantitative analysis method of an aromatic sterane compound in petroleum. The method comprises the following steps: fully dissolving and soaking crude oil with a solvent and then filtering to obtain a filtrate; heating to evaporate and concentrate the filtrate to prepare filtrate concentrate; collecting a saturated hydrocarbon group component and an aromatic group component; preparing a saturated hydrocarbon group component sample and an aromatic group component sample and storing the samples at low temperature; analyzing the two samples by using a comprehensive two-dimensional gas chromatograph and a flight time mass detector; performing data processing on spectrograms obtained in the steps to obtain corresponding retention time and peak area integration result. The analysis method can be used for performing full oil analysis and group component analysis; an orthogonal separation system thereof can completely separate the aromatic steroid hydrocarbons from alkane, so that the problems such as common rolling peak, base line interference and insufficient peak capacity can be excellently solved, and the aromatic sterane compound can be accurately measured.

The invention relates to the technical field of oil gas geochemistry, in particular to a quantitative analysis method for judging petroleum degradation grade. The quantitative analysis method comprises the following steps: a sample preparation step of dehydrating a water-containing degraded oil sample and then weighing 50-100mg of the dehydrated water-containing degraded oil sample in a sample bottle; a sample degradation grade quantitative analysis step of analyzing the sample by using comprehensive two-dimensional gas chromatography-time of flight mass spectrometry; calculating the relativemass of n-isoparaffin, cyclane, arene and sterane, and terpane compound according to a peak area of a comprehensive two-dimensional gas chromatogram to be respectively denoted as A, B, C, D, and calculating an X value, wherein X=A / (A+B+C+D), the smaller the X value is, the larger the degradation grade is. The quantitative analysis method of the invention can finely divide the crude oil with the same source and the same degradation grade, thereby classifying the crude oil for geological research.

The present invention relates to the new process of synthesizing furasteroid saponin and its derivative chemically with C27 helical sterane type sapogenin and various kinds of saccharide as material. The synthesis process includes the following steps: 1. synthesizing completely protected C27 helical steroid saponin through the reaction of C27 helical sterane type sapogenin and acyl radical, alkyl radical or silicon radical protected glycosyl donor; 2. reaction of completely protected helical steroid saponin and oxidant to obtain oxidized product; 3. reaction of oxidized helical steroid saponin and oxyphilic reagent to produce place 26 halogenated saponin derivative; 4. nucleophilic substitution reaction of nucleophilic reagent and the halogenated product to introduce XR3 radical; 5. reducing the place 16 carbonyl radical in saponin selectively with reductant; and 6. eliminating all the protecting radicals to obtain furasteroid saponin and its analog.

The invention relates to a method for separating and enriching gonane and hopane biomarkers in combination with molecular sieves, comprising the steps of 1) weighing a sample, adding the sample to a fine silica gel packed column, and leaching with n-hexane to separate out saturated hydrocarbons; 2) adding to a ZSM-5 molecular sieve packed column, and leaching with isooctane to separate out cyclic and isomeric components of the saturated hydrocarbons; 3) adding to a 13X molecular sieve packed column, leaching the column with n-pentane, and drying the column with nitrogen; 4) concentrating the solution obtained in the step 3) to a 10X molecular sieve packed column, leaching with two parts of n-pentane and collecting two fractions of gonane; 5) extracting the 13X molecular sieve for 10h with isooctane to obtain a hopane component; and 6) concentrating the solution obtained in steps 4) and 5), and measuring in a machine. The method can be used for effectively separating and enriching gonane and hopane, so that the determination of monomer hydrocarbon stable carbon isotopes of the gonane and hopane biomarkers becomes possible.

The invention provides a novel wool gonane compound as well as a preparation method and an anticancer purpose thereof. The wool gonane compound is shown as the right general formula (I); and an anticancer activity test indicates that the novel wool gonane compound has obvious anticancer activity, wherein R1 and R2 in the formula are respectively and independently selected from hydrogen and alkyl with 1-6 of carbon number.

The invention discloses harmful mycogone perniciosa 88362 with the preservation number of CGMCC NO.8352. Ergot sterane type triterpenoid prepared by fermenting the harmful mycogone perniciosa has a structural formula shown in the specification. The invention provides a new strain, two new components and a method for preparing the new compounds by utilizing the new strain, and a material base is provided for developing and utilizing natural active substances from microorganisms.

The invention discloses a method for efficient separation and recycling of 5,10-alpha epoxy isomer in telapristone synthesis. A 5,10-alpha epoxide is a key intermediate in a telapristone synthesis process, and preparation of a high-purity 5,10-alpha epoxy isomer is the key in control of a whole quality system. The 5,10-alpha isomer is efficiently separated by means of solvent pulping for the first time, the content reaches 99%, and the yield is up to 65%. More significantly, a mixture of two epoxy isomers in mother solution after separation of the 5,10-alpha epoxy isomer can be subjected to reduction and elimination reaction to return to a raw material 17beta-cyano-17alpha-trimethylsiloxy-13beta-methyl sterane-5,9-diene-3,3-(ethylenedioxy). The method is remarkable in improvement of raw material utilization efficiency and has a large-scale application prospect.

An object of the present invention is to provide a novel method for producing a steroid compound. The present invention provides a method for producing 3,7-dioxo-5beta-cholanic acid or ester derivatives thereof, which uses, as raw materials, sterols having double bonds at positions 5 and 24, such as cholesta-5,7,24-trien-3beta-ol, ergosta-5,7,24(28)-trien-3beta-ol, desmosterol, fucosterol, or ergosta-5,24(28)-dien-3beta-ol, and which comprises the following 4 steps: (I) a step of performing oxidation of a hydroxyl group at position 3 and isomerization of a double bond at position 5 to position 4; (II) a step of converting position 24 to a carboxyl group or an ester derivative thereof by the oxidative cleavage of a side chain; (III) a step of introducing an oxygen functional group into position 7; and (IV) a step of constructing a 5beta-configuration by reduction of a double bond at position 4.

The invention discloses an application of Lanosta-7,9(11),24-trien-3-one 15,26-dihydroxy in preparation of medicaments for preventing and / or treating enterovirus 71 infection. The invention confirms that the Lanosta-7,9(11),24-trien-3-one 15,26-dihydroxy has good antiviral effect in in-vitro enterovirus 71 (EV 71) infection cell tests. Meanwhile, the Lanosta-7,9(11),24-trien-3-one 15,26-dihydroxyhas certain killing effect on enterovirus 71 (EV 71), can prevent enterovirus 71 (EV 71) infection, has good treating effect on enterovirus 71 (EV 71) infected cells, has good inhibition effect on viral replication, and has more significant effect than a positive control medicament of ribavirin. In addition, the Lanosta-7,9(11),24-trien-3-one 15,26-dihydroxy has good inhibition effect on inflammation responses caused by enterovirus 71 (EV 71), which reveals that the Lanosta-7,9(11),24-trien-3-one 15,26-dihydroxy has the prospect of being developed into anti-enterovirus 71 (EV 71) medicaments.

The invention discloses an improved full-automatic solid-phase extraction instrument and application thereof to automatic separation of monoaromatic sterane. The automatic solid-phase extraction instrument comprises a solvent system, a solvent switching valve, a metering pump, a chromatographic column and a collecting system in sequence, wherein the systems are connected through a flow passage circuit; an appropriate solvent is selected by controlling the solvent switching valve to be pumped into the chromatographic column by the metering pump; all kinds of substances adhered onto a solid phase of the chromatographic column are separated; and the eluted different substances are controlled by mechanical arms of the collecting system to flow into different receiving flasks. According to a method for separating the monoaromatic sterane by the instrument, the monoaromatic sterane is completely separated from saturated hydrocarbon component and completely enters an aromatic hydrocarbon component. The method solves the problem that saturated hydrocarbon is mutually mixed with aromatic hydrocarbon in an existing method, complete separation of monoaromatic sterane substances is realized, and the improved full-automatic solid-phase extraction instrument is introduced, so that automation of sample analysis is realized, and the disadvantages of complex operation and low work efficiency for manual separation are greatly avoided.

This invention relates to a method for selectively preparing the 3-oxo-4-aza-5¥á-androstane compound which is used as an intermediate of finasteride by heating 3-oxo-4-aza-5-androstene in a mixture of formic acid and an alkanediol in the presence of zinc.

The invention provides a method for determining migration dynamic conditions by utilizing hydrocarbon-type geochemical parameters of oil deposits. The method for determining migration dynamic conditions by utilizing hydrocarbon-type geochemical parameters of the oil deposits comprises the steps that 1, classified calibration of a hydrocarbon source rock stratum and biological marker feature analysis are performed; 2, oil deposit sample analysis and oil and source comparison are performed, and the oil deposits are classified according to oil sources; 3, the change laws with depths of hydrocarbon-type sterane isomerization parameters and light-heavy ratio parameters of same-type-source oil deposits or single-source oil deposits are established, and mode curves are drawn; 4, influence factorsof trend changes of trend lines are analyzed; 5, dynamic condition analysis and distinguishing are performed, and dynamic systems are divided. The method for determining migration dynamic conditionsby utilizing the hydrocarbon-type geochemical parameters of the oil deposits utilizes multiple geochemical parameters to determine a dynamic environment for hydrocarbon migration, research of an oil-gas reservoir forming mechanism is achieved, and evidences for migration and reservoir forming research are more directly and reliably provided.

The invention provides a method for determining abnormal high pressure waves and range of a basin by utilizing sterane isomerization parameters. The method comprises the following steps: determining the change rule, evolving with the sterane isomerization parameters, in hydrocarbon source rocks, and drawing a sterane isomerization parameter evolving curve of the hydrocarbon source rocks; establishing variation tendency with depth of the sterane isomerization parameters of a monophyletic oil deposit; determining the variation tendency of the sterane isomerization parameters in a transportation process from the hydrocarbon source rocks to the oil deposit; determining hydrocarbon expulsion threshold of the hydrocarbon source rocks, calibrating and dividing a sterane isomerization parameter boundary line between abnormal high pressure and a buoyancy effect interval; carrying pressure characteristic analysis on continuous reservoir and hydrocarbon accumulation in a single well; and according to the sterane isomerization parameter boundary line, separately dividing waves and range of power on a profile and a plane. The method for determining abnormal high pressure waves and range of the basin by utilizing sterane isomerization parameters realizes quantitative characterization of power conditions of an oil-gas hydrocarbon accumulation period, defines the pressure structure, in the hydrocarbon accumulation period, of the basin, and provides technical support for quantitative research on oil-gas hydrocarbon accumulation.

The present invention relates to a method for analyzing sterane-based compounds in a petroleum geological sample. According to the method, organic matters in a rock core sample are extracted with an organic solvent, and the extracted supernatant is directly analyzed through atmospheric pressure photoionization high resolution mass spectrometry (APPI FT-ICR MS). According to the present invention,with the method, the sample consumption is low, the sample preparation process is simple, the sample extraction time is short, and the extract can be directly subjected to atmospheric pressure photoionization high resolution mass spectrometry analysis without concentration and separation so as to avoid the compound loss caused during the separation; and the atmospheric pressure photoionization high resolution mass spectrometry analysis process is accurate and rapid, the sterane-based compound carbon number distribution range detected by the high resolution mass spectrometry has high reliability and high accuracy compared to the GC-MS results, and the method for analyzing the sterane-based compounds in the petroleum geological sample is expanded.

The invention discloses chalcone derivatives containing allyl structures, and application of the chalcone derivatives. 39 novel allyl-progeny steranes, based on licochalcone A and E used as pre-molecules are synthesized. As a PTP1B inhibitor, the chalcone derivatives can improve the treatment of common diabetes mellitus type 2. Most synthetic compounds show potent inhibition on PTP1B.

The invention discloses a chalcone derivative containing an allyl structure and its application. The invention synthesizes 39 new allyl-post-steranes based on Licochalcone A and E as promolecules. As a PTP1B inhibitor, it may improve the common treatment of type 2 diabetes. Most of the synthetic compounds showed potent inhibition of PTP1B.

A crude oil density prediction method based on saturated hydrocarbon biomarker parameters, comprising: collecting crude oil saturated hydrocarbon biomarkers, including n-alkanes series, terpanes series, sterane series and alkylcyclohexane series biomarkers identification and Relative quantification; calculate crude oil saturated hydrocarbon parameters; establish a fitting formula based on the correlation between crude oil saturated hydrocarbon parameters and surface crude oil density; predict the surface crude oil density of the target oil-bearing formation according to the fitting formula. The purpose of the present invention is to predict the surface crude oil density of the target oil-bearing layer system by gas chromatography-mass spectrometry analysis of the saturated hydrocarbon components of the wall core or a small amount of rock core extract. The invention performs fitting and forecasting of medium and light oil and heavy oil in sections, and has the advantages of simple operation, low cost, high precision and few affected factors.

The invention relates to a molecular sieve separating and preparing method for sterane and diahopane. 60mg of crude oil or rock extracts are prepared, 6g of solica, the particle diameter of which is 40 micrometers, is used for filling columns, and 15ml of n-hexane is used for preparing saturated hydrocarbon in a leaching manner; ZSM molecular sieve with the weight about 100 times the weight of the saturated hydrocarbon is used for filling columns and 20ml of isooctane is used for preparing cyclic hydrocarbon in the leaching manner; 10X molecular sieve with the weight about 300 times the weight of the cyclic hydrocarbon is used for filling columns, and after 30 minutes, 80ml of n-pentane is used for preparing fractions; the solvent of fractions is recovered and volatilized, the fractions are then weighted, 10X molecular sieve with the weight about 200 times the weight of the fractions is used for filling columns and after 30 minutes 10ml is used for preparing sterane in the leaching manner; the filling materials in the third step is dried by the nitrogen, which are then extracted by the isooctane for 12 hours to get diahopane. The separation effect is good after the mass detection of a chromatograph, which is similar to the effect of the separation spectrogram exhibited in foreign countries and the operation of the enriching and separating method of diahopane is simple.

The invention provides a comprehensive two-dimensional gas chromatography quantitative analysis method of an aromatic sterane compound in petroleum. The method comprises the following steps: fully dissolving and soaking crude oil with a solvent and then filtering to obtain a filtrate; heating to evaporate and concentrate the filtrate to prepare filtrate concentrate; collecting a saturated hydrocarbon group component and an aromatic group component; preparing a saturated hydrocarbon group component sample and an aromatic group component sample and storing the samples at low temperature; analyzing the two samples by using a comprehensive two-dimensional gas chromatograph and a flight time mass detector; performing data processing on spectrograms obtained in the steps to obtain corresponding retention time and peak area integration result. The analysis method can be used for performing full oil analysis and group component analysis; an orthogonal separation system thereof can completely separate the aromatic steroid hydrocarbons from alkane, so that the problems such as common rolling peak, base line interference and insufficient peak capacity can be excellently solved, and the aromatic sterane compound can be accurately measured.

The invention discloses a preparation method of a rocuronium bromide intermediate 5alpha-sterane-2-ene-17-one. The specific steps include: sequentially adding epiandrosterone TS, a solvent and a phasetransfer catalyst into a reaction bottle, performing heating to a reaction temperature of 90-120DEG C, carrying out stirring reaction, and conducting post-treatment purification to obtain the 5alpha-sterane-2-ene-17-one. The method provided by the invention greatly shortens the reaction time, reduces byproducts, enhances the yield, reduces three wastes, lowers the production cost, is suitable forindustrial production, and has obvious social and economic benefits.

The invention discloses a method for efficient separation and recycling of 5,10-alpha epoxy isomer in telapristone synthesis. A 5,10-alpha epoxide is a key intermediate in a telapristone synthesis process, and preparation of a high-purity 5,10-alpha epoxy isomer is the key in control of a whole quality system. The 5,10-alpha isomer is efficiently separated by means of solvent pulping for the first time, the content reaches 99%, and the yield is up to 65%. More significantly, a mixture of two epoxy isomers in mother solution after separation of the 5,10-alpha epoxy isomer can be subjected to reduction and elimination reaction to return to a raw material 17beta-cyano-17alpha-trimethylsiloxy-13beta-methyl sterane-5,9-diene-3,3-(ethylenedioxy). The method is remarkable in improvement of raw material utilization efficiency and has a large-scale application prospect.

The invention discloses an androstane-3β-hydroxy-17-ketone-6α-boronic acid obtained by separating androstane-3β-hydroxy-17-ketone-6α-boronic acid from a chemical reduction reaction solid waste of androst-5-ene-17-ketone through esterification, oxidation and sulfonic acid. A method for preparing 3β-acetoxyandrost-5-ene-17-ketone by esterification and elimination four-step reaction. The method of the invention can effectively recycle and reuse the steroid solid waste, the synthesis method is efficient, the reaction conditions are mild, the environmental pollution is reduced, and the cost of related products is correspondingly reduced.

The present invention relates to the new process of synthesizing furasteroid saponin and its derivative chemically with C27 helical sterane type sapogenin and various kinds of saccharide as material. The synthesis process includes the following steps: 1. synthesizing completely protected C27 helical steroid saponin through the reaction of C27 helical sterane type sapogenin and acyl radical, alkyl radical or silicon radical protected glycosyl donor; 2. reaction of completely protected helical steroid saponin and oxidant to obtain oxidized product; 3. reaction of oxidized helical steroid saponin and oxyphilic reagent to produce place 26 halogenated saponin derivative; 4. nucleophilic substitution reaction of nucleophilic reagent and the halogenated product to introduce XR3 radical; 5. reducing the place 16 carbonyl radical in saponin selectively with reductant; and 6. eliminating all the protecting radicals to obtain furasteroid saponin and its analog.

The invention relates to a method for separating gonane and hopane from crude oil or rock extract, which comprises: passing 80 milligrams of crude oil or rock extract through 6 grams of silica gel-packed column with a particle diameter of 100 to 200 meshes, and leaching with 15 milliliters of n-hexane to obtain saturated hydrocarbons; volatilizing solvent on a nitrogen evaporator, weighing a saturated hydrocarbon, passing the saturated hydrocarbon through zeolite sieve of molecular porosity (ZSM) molecular sieve-packed column of which the mass is 100 times that of the saturated hydrocarbon, leaching with 20 milliliters of isooctane to obtain an isohydrocarbon; volatilizing solvent on a nitrogen evaporator, and weighing the saturated hydrocarbon; weighing 3.0 grams of NaY molecular sieve-packed column, and allowing 8.0 milligrams of isohydrocarbon to be absorbed on about 80 milligrams of coarse silica gel; transferring the isohydrocarbon onto the NaY molecular sieve column by using cold n-pentane and leaching with cold n-pentane; and collecting diffident required samples according to different amounts, namely collecting gonane first and then collecting hopane. The result of chromatography-mass spectrometry shows that the separation effect is desirable.

The invention discloses an application of Lanosta-7,9(11),24-trien-3-one 15,26-dihydroxy in preparation of medicaments for preventing and / or treating enterovirus 71 infection. The invention confirms that the Lanosta-7,9(11),24-trien-3-one 15,26-dihydroxy has good antiviral effect in in-vitro enterovirus 71 (EV 71) infection cell tests. Meanwhile, the Lanosta-7,9(11),24-trien-3-one 15,26-dihydroxyhas certain killing effect on enterovirus 71 (EV 71), can prevent enterovirus 71 (EV 71) infection, has good treating effect on enterovirus 71 (EV 71) infected cells, has good inhibition effect on viral replication, and has more significant effect than a positive control medicament of ribavirin. In addition, the Lanosta-7,9(11),24-trien-3-one 15,26-dihydroxy has good inhibition effect on inflammation responses caused by enterovirus 71 (EV 71), which reveals that the Lanosta-7,9(11),24-trien-3-one 15,26-dihydroxy has the prospect of being developed into anti-enterovirus 71 (EV 71) medicaments.

The present invention relates to a method for analyzing sterane-based compounds in a petroleum geological sample. According to the method, organic matters in a rock core sample are extracted with an organic solvent, and the extracted supernatant is directly analyzed through atmospheric pressure photoionization high resolution mass spectrometry (APPI FT-ICR MS). According to the present invention,with the method, the sample consumption is low, the sample preparation process is simple, the sample extraction time is short, and the extract can be directly subjected to atmospheric pressure photoionization high resolution mass spectrometry analysis without concentration and separation so as to avoid the compound loss caused during the separation; and the atmospheric pressure photoionization high resolution mass spectrometry analysis process is accurate and rapid, the sterane-based compound carbon number distribution range detected by the high resolution mass spectrometry has high reliability and high accuracy compared to the GC-MS results, and the method for analyzing the sterane-based compounds in the petroleum geological sample is expanded.

The invention discloses a rapid crude oil and organic matter saturated hydrocarbon complexation analysis method. By performing complex formation separation on crude oil and organic matter saturated hydrocarbons by virtue of the method disclosed by the invention, n-alkanes as well as cycloalkanes and isoalkanes can be effectively separated, and concentration computer detection is performed on the cycloalkanes and the isoalkanes, so that biological marker compounds such as sterane and terpane required by research can be detected, spectrograms before and after complexation are compared, and resolutions of the biological marker compounds are effectively improved on the basis that chromatographic peaks and biological marker parameters are basically consistent, so that research needs are met.