61 results about "Post infection" patented technology

The invention discloses a surface antigen gene StSAG1 of Sarcocystis tenella, the nucleotide sequence of the surface antigen gene StSAG1 is as shown in SEQ ID NO: 1, and according to the analysis result of protein antigenicity and antigen epitope, the 686 bp-1175 bp segment of the StSAG1 gene is selected to construct a recombinant protein expression vector and engineering bacteria; the antigen disclosed by the invention has very strong antigenicity and specificity, and can be subjected to effective antigen-antibody reaction with an antibody generated in serum after sheep (Ovis aries) is infected with sarcosporidium tenella, so that diagnosis is realized; low-titer antibodies can be detected two weeks after infection, and the detection window period is obviously advanced; by adopting the ELISA detection method, a visual result can be directly obtained. The method breaks through the limitation of an infection window period, has the advantages of low cost, high sensitivity, simplicity and convenience in operation, no need of expensive instruments and analysis and the like, is suitable for field detection in primary laboratories and pastures, and is beneficial to industrial production and market popularization and application.

The invention discloses a construction and verification method of an HEK293T cell line capable of stably overexpressing human ANT2, which comprises the following steps: cloning a human ANT2 gene, constructing the human ANT2 gene into a lentivirus expression plasmid pLVX-TRE3G, co-transfecting the lentivirus expression plasmid pLVX-TRE3G and a helper plasmid pLVX-Tet3G into an HEK293T-ACE2 cell, and carrying out puromycin and G418 pressurized screening and Western blot identification to obtain the HEK293T cell line capable of stably overexpressing human ANT2. A result shows that the expression quantity of the ANT2 is the highest when the HEK293T-ACE2-ANT2 cell is induced for 12 hours at the Doxycycline of 6mg / L; when the ANT2 is induced to express, the virus nucleic acid load and the N protein expression level after SARS-CoV-2 infection are obviously reduced, which indicates that the ANT2 has an inhibition effect on SARS-CoV-2 infection replication, and a cell model and an experimental basis are provided for exploring the effect of the ANT2 in virus infection resistance.

The invention relates to a method for detecting activation of specific T cells after tuberculosis infection and application of the method. Specifically, the invention provides a reagent combination for detection, and the reagent combination comprises an anti-CD4 antibody (CD4 antibody), an anti-CD154 antibody (CD154 antibody), an IFN-gamma detection antibody and a tuberculosis specific antigen. According to the method, through peripheral whole blood collection, PBMC cell separation, T cell activation (activation), activation termination (blocking), antibody labeling, membrane rupture, fixation, intracellular cytokine dyeing, flow cytometry detection and result analysis and judgment, the ratio of the expression IFN-gamma cells in the CD4 + CD154 + cells can be rapidly and accurately determined, and by combining an ROC curve and a constructed judgment standard, the content of the IFN-gamma cells in the CD4 + CD154 + cells can be determined, and the content of the IFN-gamma cells in the CD4 + CD154 + cells in the CD4 + CD154 + cells in the CD4 + CD154 + cells in the CD4 + CD154 + cells can be determined. And the sample to be detected is judged to be in a positive / negative / gray region, so that whether a patient is infected by the mycobacterium tuberculosis or not can be more accurately diagnosed, especially aiming at a whole blood sample from a special crowd.

The invention discloses application of Med26 gene / protein as a target spot in preparation of a medicine for preventing and / or treating BCG infection, and belongs to the technical field of biological medicine. Aiming at the technical problems that the host defense defect is caused by excessive death of macrophages when a host is infected with BCG, the research on the mechanism of regulating and controlling the macrophages to resist BCG infection by host genes is not perfect and the like at present, a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) screening technology is utilized to discover the key effect of the Med26 gene for negatively regulating and controlling the survival of the macrophages in the BCG infection in a BCG infection model for the first time, so that the BCG infection resistance of the host is improved. And it is verified that the survival rate of the macrophage after BCG infection can be significantly improved through targeted inhibition of Med26 gene expression, and the BCG removing capacity of the macrophage is enhanced. The discovery of the invention provides a new target for research and development of drugs for preventing and / or treating BCG infection.

The invention discloses a real-time detection system and a monitoring and early warning method for African swine fever virus. The system comprises a latex microsphere labeled immunochromatography antibody detection card, a portable MINI-PCR instrument and a freeze-dried microsphere type fluorescent PCR detection reagent, the detection card takes the recombinant ASFV p30 protein as a target spot, antibody detection is completed within 10 minutes, the lowest detection limit is 1: 1024, and the specificity is greater than 99%; the PCR reagent adopts a double-primer double-probe design, and the lowest detection limit is 1 copy / microliter. According to the invention, through a collaborative process of antibody screening, nucleic acid detection and result evaluation, ASFV total infection cycle detection is realized, epidemic situation discovery time is advanced to 1-3 days after infection, and early warning response time is shortened by more than 60%; the system is simple to operate and low in cost, does not need professionals and laboratories, can be deployed in basic-level scenes such as pig farms and slaughter houses, effectively solves pain points of an existing detection system, and provides key technical support for prevention and control of African swine fever.

The invention relates to the technical field of aquatic product culture, and particularly discloses application of zinc in preparation of a product for preventing and treating siniperca chuatsi rhabdovirus infection. By adding a proper amount of zinc ions into a mandarin fish cell culture system or culture environment, the application of zinc after infection in forms of zinc sulfate and the like can significantly inhibit protein expression and virus proliferation of the mandarin fish rhabdovirus, and has no toxic or side effect on host cells within a certain concentration range. The novel nutritional immune prevention and control strategy which is safe, economical and easy to operate provides an effective means for prevention and control of siniperca chuatsi virus diseases, and has important application prospects and economic values.

The invention discloses a pharmaceutical composition for treating post-infection cough and a preparation method thereof. The traditional Chinese medicine composition provided by the invention dispels wind and freezes lung to relieve exterior syndrome, eliminates dampness and strengthens spleen to treat middle energizer and depresses qi and reduces phlegm to relieve cough, and the whole formula combines dredging and tonifying and orderly freeing and descending, and embodies the formula characteristics of simultaneous regulation of lung and spleen and simultaneous treatment of principal and subordinate symptoms. The medicines are matched to dispel lung channel wind to stop spasm, and also can dissipate dampness with aromatics, invigorate the spleen to eliminate phlegm sources, tonify qi to strengthen superficies to tonify deficiency, and achieve the effects of dispelling wind and ventilating lung, eliminating dampness and phlegm, depressing qi and relieving cough. The traditional Chinese medicine composition directly focuses on the pathogenesis of cough with wind evil with dampness and adverse rising of lung qi after infection, and can be used for treating the symptoms of cough with exuberant wind and dampness obstruction after infection, such as throat itching and cough, frequent and severe cough, sticky expectoration, chest distress, epigastric fullness, fatigue and the like. The invention provides a new treatment strategy for the post-infection cough, and has a wide clinical application prospect.

The invention discloses a Ruditapes philippinarum miR-263b inhibitor and application thereof, and finds that Ruditapes philippinarum miR-263b can regulate and control innate immune response of a host by targeting RpSC2, negatively regulates activation of an immune-related signal channel in a vibrio infection process, and further, on the basis of a molecular regulation and control mechanism, the Ruditapes philippinarum miR-263b inhibitor can be used for inhibiting the infection of vibrio. The invention discloses a specific inhibitor which is completely complementary with a miR-263b mature body sequence. Experimental data show that after Ruditapes philippinarum is injected with the miR-263b inhibitor, the expression level of miR-263b in vivo is reduced, and the inhibition effect of miR-263b on RpSC2 is effectively relieved, so that the expression level of a target gene RpSC2 is up-regulated, an NF-kappa B immune signal channel is activated, the immune defense capability of the Ruditapes philippinarum on vibrio anguillarum is enhanced, the death rate after infection is remarkably reduced, and the Ruditapes philippinarum is prevented from being infected. The in-vivo bacterium loading amount is obviously reduced, and the pathological injury of hepatopancreas tissue is obviously relieved.

The invention relates to the technical field of biological medicines, in particular to a maintenance solution for culturing influenza viruses as well as a preparation method and application of the maintenance solution. The maintenance liquid comprises a basic culture medium and effective components, the effective components comprise amino acids, vitamins, salts and a virus nutrition enhancer, and the virus nutrition enhancer is a microorganism induced culture extract. The extract is prepared by inoculating fermentation microbial bacteria, performing induced culture through a plant induction factor extract containing American ginseng, polygonum cuspidatum and golden cypress, and then performing autolysis and enzymolysis in the presence of compound metabolic regulatory factors, namely trichosalix A and N-acetylcysteine. The maintenance fluid can significantly enhance host cell metabolism, inhibit cell antiviral response and delay cell apoptosis after infection, so that the culture yield and process stability of influenza viruses are significantly improved under the condition of no dependence on serum.

The present invention relates to a pharmaceutical composition comprising creatine or physiologically acceptable derivatives and / or salts and or adducts thereof for use in the treatment of breathing difficulties, chest pain, body aches etc. after virus infection particularly in combination with breathing exercises. A further embodiment of the invention relates to the use of a composition comprising creatine or physiologically acceptable derivatives and / or salts and or adducts thereof as dietary supplement for preparation of a diet supporting the recovery from breathing difficulties, chest pain, body aches etc. by breathing exercises after a viral infection of the lungs or lower respiratory system.

Embodiments of therapeutic protocols to treat Post-Acute Sequelae SARS-CoV-2 infection (“PASC”), a.k.a. “long Covid,” are described. The PASC treatment protocols focus on a moderating a hyperimmune response; destroying and removing the SARS CoV-2 spike protein from the gut and body, detoxifying the body and the brain; replenishing key nutrients; mitigating depression and anxiety; and a regimen of physical and mental exercises. A standard six-week protocol and a shorter, three-week protocol are disclosed for those with a milder form of PASC are disclosed.

The invention discloses application of neochlorogenic acid in preparation of a medicine for preventing and / or treating hand-foot-and-mouth disease caused by A16 type coxsackie virus (CVA16) infection, and belongs to the technical field of biological medicine. Researches prove that the neochlorogenic acid has the effect of resisting CVA16 infection on HEK293T cells and H4 cells, after the two cells treated by the neochlorogenic acid are infected by the CVA16, the viral genome RNA abundance is obviously reduced, and the cell lesion death condition is obviously relieved; meanwhile, a cytotoxicity test shows that the neochlorogenic acid has no obvious cytotoxicity to HEK293T cells and only has weak cytotoxicity to H4 cells. Therefore, in a reasonable dosage range, the neochlorogenic acid, which is a low-toxicity natural small molecule compound, can be used for preparing the anti-CVA16 medicine for preventing and / or treating the hand-foot-and-mouth disease caused by CVA16 infection.

Peptides generating antibodies to human immunodeficiency virus (HIV) post-infection were identified. Higher antibody reactivity to these peptides was associated, with lower viral load post-infection.

The inventions relate to compounds and compositions comprising connexin hemichannel modulators, inflammasome modulators, and / or pannexin channel modulators and methods for the treatment of post-acute infection disorders, including long COVID, long flu, and post-treatment Lyme disease syndrome.

The present application belongs to the field of biomedical technology, and relates to a human papilloma virus (HPV) infection immune response related detection method and application. The present application provides a new method for detecting HPV infection, which comprises detecting virus load, DC maturation state in peripheral blood, or T lymphocyte subgroups and NK cell surface markers. The present application adopts a combined detection technology of DC cells, T lymphocytes and NK cells, and is used for detecting the immune response state of a host after HPV infection. The present application also provides application of the detection method. The detection method of the present application is simple and clear in result, and can be applied to clinical auxiliary detection and examination of HPV and even cervical cancer, and screening of HPV and even cervical cancer.

The invention relates to the technical field of biological medicine, in particular to a construction method and application of a respiratory virus infected alveolar organ model. Comprising the following steps: infecting alveolar cell source single cells by using a virus inoculum, coating the infected alveolar cell source single cells with matrigel, and inoculating the infected alveolar cell source single cells into a first culture medium for three-dimensional culture to obtain the respiratory virus infected alveolar organ model. According to the invention, alveolar cells are creatively applied to model characterization of respiratory virus infection, and particularly, alveolar cell source single cells are subjected to infection treatment by adopting influenza A H1N1 virus, so that the constructed model can well simulate and characterize the infection process of influenza A virus in human lung organs; the method provides an experimental basis for drug research and development (such as high-throughput screening of anti-influenza virus drugs), viral disease pathogenesis research, research on defense mechanisms of naturally occurring virus infection or virus infection caused by experiments and the like, and has important guiding significance.

The invention provides a monoclonal antibody aiming at schistosoma japonicum SPRM1hc protein and application of the monoclonal antibody, and belongs to the technical fields of parasite infection treatment, biological medicine, immunotherapy and in-vitro diagnosis. The invention provides two monoclonal antibodies A028 and A032 which can be specifically combined with the extracellular domain of SPRM1hc protein, the actual effect of the monoclonal antibodies A028 and A032 in treatment of schistosoma japonicum katsurada after infection is verified, and the monoclonal antibodies A028 and A032 have a remarkable treatment effect in an infected animal model, and can reduce polypide load, reduce egg deposition and improve tissue pathological damage. The application of the monoclonal antibody to preparation of drugs for resisting schistosoma japonicum katsurada infection and development of schistosoma japonicum katsurada antigen detection kits has very important practical significance.

The application relates to the technical field of virus genetic engineering, and particularly relates to a construction method of a porcine respiratory syndrome virus. The construction method is as follows: taking a PRRSV HEB1 strain genome (GenBank EF112447.1) as a template and modifying the same, then cloning the modified genome into a pBAC11 carrier, carrying out in-vitro transcription and transfecting Marc-145 cells, and rescuing a recombinant virus rPRRSV-HA-CMV-mC. The recombinant PRRSV strain shows significant replication delay and titer reduction, forming an attenuated phenotype. Further, the recombinant virus is emulsified with an ISA206 oil adjuvant to prepare a vaccine, which shows high immunogenicity in piglet immunization, can significantly reduce clinical symptoms, viremia and lung pathological damage after PRRSV infection, and has the potential to be developed into a safe and effective vaccine.

The inventions relate to compounds and compositions comprising connexin hemichannel modulators, inflammasome modulators, and / or pannexin channel modulators and methods for the treatment of post-acute infection disorders, including long COVID, long flu, and post-treatment Lyme disease syndrome.

The invention provides a vaccine composition which comprises A and B subtype RSV antigens and an oil-in-water emulsion adjuvant, and relates to the technical field of biological medicines. The vaccine composition disclosed by the invention can induce an organism to generate high-level neutralizing antibodies and binding antibodies, greatly reduces the virus load of nasal oracle bones and lung tissues after RSV infection, relieves pathological damage of RSV to the lung tissues, and effectively protects cross infection caused by different strains of RSV.

To provide a method for controlling intracellular parasitic bacterial infections in cultured fish, in which repeated administration of an immunostimulant in combination with an antibiotic maximizes the efficacy of the antibiotic and minimizes the onset of intracellular parasitic bacterial infections with a short dosing period.SOLUTION: An antibiotic is administered at predetermined intervals together with feeding of an immunostimulant to young-of-the-year cultured fish. By repeatedly performing both natural infection with target intracellular parasitic bacteria and treatment after infection multiple times, the young-of-the-year cultured fish acquire immunity against the intracellular parasitic bacteria, thereby suppressing the onset of intracellular parasitic bacterial infections in two-year-old cultured fish.SELECTED DRAWING: None

The invention discloses a targeted sequencing primer composition for tuberculosis-related pathogens and a kit. The invention provides a mycobacterium tuberculosis targeting sequencing primer composition aiming at tuberculosis pathogens on the basis of a pathogen targeting sequencing technology. On the basis, a reliable basis is provided for better distinguishing tuberculosis from a'tuberculosis-like 'disease and clinical diagnosis and treatment of tuberculosis, non-tuberculosis mycobacteria and other pathogens capable of causing symptoms similar to tuberculosis after infection are incorporated, and a corresponding targeted sequencing primer composition and a kit are provided. The pathogens detected by the targeted sequencing primer composition and the kit for tuberculosis-related pathogens are relatively comprehensive, rapid and accurate screening and detection of mycobacterium tuberculosis and nontuberculosis mycobacterium infection are facilitated, and reliable basis can be provided for diagnosis and treatment of tuberculosis and other infection similar to tuberculosis; the important clinical significance is realized.

The invention relates to the field of veterinary protozoology and methods for producing antigens, particularly for the diagnosis of Surra Su-Auru in animals. The objective of the invention is to develop a method for producing a highly sensitive trypanosomal antigen for the serological diagnosis of Surra (Su-Auru) in animals. The problem is solved by the fact that the method for obtaining trypanosome antigen for serological diagnostics of Surra (Su-Auru) in animals involves infecting rabbits intratesticularly with live trypanosomes of the T species. evansi, removal of the spleen 2-3 days after infection, total bleeding 8-10 days after splenectomy (parasitemia -80-90%), blood is collected in a wide vessel with 2% sodium citrate in physiological solution with constant stirring with a glass rod, at the rate of 1 part blood to 2 parts solution, settling of citrated blood in the refrigerator for 18-24 hours, centrifugation and separation of the middle layer from the sediment from the supernatant, 2-3 times washing of trypanosomes in physiological solution, at 3000-5000 rpm, for 10 minutes each, disintegration of the centrifugate by ultrasound at a frequency of 22 kHz, power of 60 W / cm2 for 8-12 minutes, followed by centrifugation of the disintegrate at 8000-10000 rpm for 20-30 minutes and collection of sediment (endoantigen), treatment of the supernatant with TCA, collection of the precipitated sediment (exoantigen), batches of sediment are mixed 10:1. The technical result provided by the utility model is expressed in the development of a method for obtaining a highly sensitive trypanosomal antigen for the serological diagnosis of Surra (Su-Auru) in animals.

Methods, systems and devices are provided to stimulate the vestibular system in such a way as to alleviate post-infection symptoms of COVID-19. A device with one or more electrodes placed over a subject's scalp provides vestibular nerve stimulation (VeNS) to the vestibular nerve, which is then carried into the vestibular nucleus in the brainstem to alleviate post-infection symptoms of COVID-19 related to the vestibular system, including dizziness, fatigue, malaise, headache, nerve pain, insomnia and sleep problems, anxiety, depression and difficulty concentrating. The characteristics of the stimulation signal and duration of the treatment are configured to allow the treatment to be delivered at the onset of one or more symptoms or at regular daily intervals alleviate the post-infection symptoms immediately or over a longer period of time.

This document relates to methods and materials involved in treating a mammal having a gastrointestinal disorder (e.g., an irritable bowel syndrome (IBS) such as post-infection IBS (PI-IBS)). For example, one or more protease inhibitors and / or one or more microorganisms that produce one or more protease inhibitors can be administered to a mammal having, or at risk of developing, a gastrointestinal disorder to treat the mammal.

The invention discloses application of a micropterus salmoides type 2 interferon related factor IFN-gamma rel in preparation of a medicine for treating and / or preventing iridovirus infection of micropterus salmoides. Through screening of transcriptome data after LMBV infection of micropterus salmoides and qPCR verification, expression of IFN gamma rel after LMBV infection is significantly up-regulated. The recombinant bacillus subtilis B.s-IFN-gammarel for expressing the protein is obtained by carrying out codon optimization on the gene of IFN gammarel, connecting the gene of IFN gammarel into plasmids to construct recombinant plasmids, and carrying out transformation and screening. Results show that the B.s-IFN-gammarel, as an antiviral oral drug for preventing or treating LMBV infection, can significantly improve the survival rate of micropterus salmoides, and has good safety. Therefore, the invention provides the application of IFN gammarel as an oral antiviral drug for preventing and treating LMBV infection in micropterus salmoides culture.

A novel recombinant subunit vaccine composition against avian Mycoplasma infection is described. The vaccine includes VlhA early phase antigens chosen based on their expression profile during the first 7 days of infection. The vaccine composition is shown to induce a protective immune response in chickens. Vaccine compositions and methods of making and using the vaccine compositions are described.

The present invention relates to a label-free, high throughput method for determining the titer of an infectious virus in an aqueous sample comprising the infectious virus wherein the method comprises detecting the kinetics of morphological cell alterations, such as cell rounding, following virus infection, determining parameters defining a curve of morphological cell changes as a function of post-infection time, and determining the infectious virus titer based on a standard curve of the infectious virus at a known infection titer.

The invention relates to the technical field of riemerella anatipestifer detection, and provides a biomarker and a detection method for detecting riemerella anatipestifer, and the biomarker is a host mucosal lesion product generated by induction of specific virulence factors of riemerella anatipestifer after duck infection. Comprising a mucoprotein cracking fragment containing a linear epitope GTTPSPVP, a sialylated sugar chain containing a specific sialic acid modification motif, and a Muc2 degradation peptide fragment. The detection method comprises the following steps: collecting a sample, pretreating, detecting corresponding markers for excrement, respiratory tract lavage fluid and mucous membrane scrape samples by adopting ELISA (Enzyme-Linked Immunosorbent Assay), an immunochromatography test strip and a lectin chip respectively, and judging a result according to a set threshold value. The kit is high in specificity, covers the whole infection period, can realize early and on-site rapid detection, and improves the diagnosis reliability.