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Kit for detecting norovirus I, norovirus II and rotavirus

A rotavirus and kit technology, which is applied in the field of molecular biology, can solve the problems of inability to meet the requirements of rapid detection and distinguish between three pathogens, etc., so as to solve the problems of instrument compatibility and residual pollution of products, strong specificity, and guarantee of timeliness. Effect

Inactive Publication Date: 2019-10-08
张鹏
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the products on the market that detect a single pathogen cannot meet the needs of rapid detection and differentiation of three pathogens

Method used

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  • Kit for detecting norovirus I, norovirus II and rotavirus
  • Kit for detecting norovirus I, norovirus II and rotavirus

Examples

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Embodiment 1

[0056] Example 1: The kit for detecting Norovirus type I, Norovirus type II and rotavirus in this example consists of a mixed reaction (2X) (including Taq hot start DNA polymerase, UNG enzyme, TaqMan Fluorescent probe, PCR buffer, dATP, dUTP, dCTP, dGTP, MgCl2, etc.), enzyme mix (20X) (Luna warm-start reverse transcriptase and mouse RNase inhibitor), forward primer, reverse primer, positive Control product and RNase-free water; wherein, the mixed reaction solution includes primers and TaqMan fluorescent probes with specific conserved sequences of Norovirus type I, Norovirus type II and rotavirus;

[0057] The primer sequence of the specific conserved sequence is:

[0058] Norovirus type I, SEQ ID NO.1 and SEQ ID NO.2;

[0059] Norovirus type II, SEQ ID NO.4 and SEQ ID NO.5;

[0060] Rotavirus, SEQ ID NO.7 and SEQ ID NO.8;

[0061] The TaqMan probe sequence of the specific conserved sequence is:

[0062] Norovirus type I, SEQ ID NO.3;

[0063] Norovirus type II, SEQ ID NO....

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Abstract

The invention belongs to the technical field of nucleic acid detection kits (fluorescent PCR (polymerase chain reaction) method) of a norovirus I, a norovirus II and a rotavirus, relates to the fieldof molecular biology, in particular to a kit for detecting the norovirus I, the norovirus II and the rotavirus and a use method of the kit. The kit solves the technical problems that the norovirus I,the norovirus II and the rotavirus belong to main pathogens of diarrhea, transmission routes, post-infection symptoms and the like are exactly similar, and an individual pathogen detection product inthe market cannot rapidly detect and distinguish three pathogens. The technical scheme includes that according to the principle of the fluorescent PCR method and nucleotide sequence characteristics, specific conserved sequences of the three viruses are selected, and corresponding primers, namely, TaqMan fluorescent probes are designed. The kit is mainly used for specimens such as excrement and cloacal swabs or vomituses, and nucleic acid of the three viruses is qualitatively detected.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a kit for detecting norovirus type I, norovirus type II and rotavirus and a use method thereof. Background technique [0002] Rotavirus (RV) is a double-stranded RNA virus belonging to the Reoviridae family. There are seven types of rotaviruses, numbered A, B, C, D, E, F, and G in English letters. Among them, type A is the most common one. Rotavirus is one of the main pathogens that cause diarrhea in infants and young children. It mainly infects small intestinal epithelial cells, causing cell damage and diarrhea. Rotavirus is prevalent in summer, autumn and winter every year, and the route of infection is the fecal-oral route. Rotavirus infection ranges from asymptomatic, mild onset to severe onset, and fatal gastroenteritis, dehydration and electrolyte balance occur in severe cases. Among them, the clinical manifestations of acute gastroenteritis include fever, vomiting, abdo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6851C12N15/11
CPCC12Q1/701C12Q1/6851C12Q2521/107C12Q2561/101C12Q2531/113C12Q2521/531C12Q2547/101
Inventor 张鹏
Owner 张鹏
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