70results about How to "Guaranteed Features" patented technology

The invention discloses a process monitoring method for an abnormal operation state in a safe water treatment process. The method comprises the following steps: firstly, segmenting original data from the dimension of time by adopting a sequential division method, and standardizing the interiors of blocks; then, a multi-scale feature extraction network is adopted to extract restrictive multi-scale spatio-temporal features from spatial dimensions by adding network function independence and information diversity constraints; and a deep stationary subspace network is established, and decoupling of stationary and non-stationary features is realized by applying difference constraints to network output features. And finally, constructing a process state change statistic by using the non-stationary features, performing data reconstruction by using the stationary features reflecting the process equilibrium state, constructing a statistic of a residual space, and completing process monitoring of the non-stationary process. According to the method, non-stationary faults influencing process safety and stationary faults caused by process mechanism abnormity can be effectively identified, and the method has high practical application value and effectiveness.

The invention relates to the field of in-vitro immunodiagnosis detection, in particular to an IgG antibody, an IgM antibody and a method. The invention provides an IgG (immunoglobulin G) antibody specifically bound with coxsackie virus, which is characterized in that amino acid sequences of complementary determining regions CDRH1-CDRH3 of a heavy chain variable region of the IgG antibody are respectively shown as SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3; the amino acid sequences of the complementary determining regions CDRL1-CDRL3 of the light chain variable region are respectively as shown in SEQ ID NO: 4, SEQ ID NO: 5 and SEQ ID NO: 6. The IgG antibody provided by the invention not only has good specificity and can specifically recognize the coxsackie virus, but also has high sensitivity and stability.

The invention relates to the technical field of bridge construction, and provides a construction method of a cable-stayed-continuous cooperative system composite beam bridge, which does not need to arrange a large batch of prestressed corrugated pipes and steel beams to ensure the use performance of a bridge deck slab, fully utilizes the structural characteristics of the cable-stayed-continuous cooperative system composite beam bridge, and improves the construction efficiency of the cable-stayed-continuous cooperative system composite beam bridge. By changing the overlapping sequence of the continuous composite beam bridge deck slabs, it is guaranteed that the bridge deck slabs in the hogging moment area of the continuous composite beam do not generate tensile stress, the bridge deck slabs in the sagging moment area generate pressure stress reserve, and by increasing the tension stay cable force, the continuous composite beam moves upwards close to a side main beam of the cable-stayed bridge and moves downwards close to a side span side main beam; the bridge deck slab generates compressive stress, the steel main beam generates tensile stress, the compressive property of concrete materials and the tensile property of steel are fully played, the material utilization rate is increased, the compressive pre-stress of the bridge deck slab is completed through internal force distribution of the composite beam, the construction method is simple and operable, the structural stress is clear, and the reasonability and safety of the structural stress are guaranteed.

The invention provides a nighttime image rain removal method based on global semantic guidance, and the method comprises the steps: 1, generating an RGB-infrared dual-mode nighttime rain image data set # imgabs0 #; 2, constructing an RGB image rain removal model based on infrared guidance, and inputting the RGB image # imgabs1 # with rain and the corresponding infrared image # imgabs2 # into an RGB image rain removal model based on infrared guidance for convolution, feature fusion, coding, decoding, feature compensation and selection, and outputting an image after preliminary rain removal. And 3, introducing the global semantic guidance module into the RGB image rain removal model, and outputting a final rain-removed image. According to the constructed IRDeNet method, different types of convolutional neural networks and attention mechanisms including a space attention mechanism, a channel attention mechanism and a self-attention mechanism are combined, and the global and local feature modeling and extraction capability of the model is ensured; the RGB image and the infrared image are fused to be used for a night image rain removal task, and the rain removal effect is remarkably improved.

The invention relates to a fruit and vegetable juice beverage, and belongs to the technical field of beverages, the fruit and vegetable juice beverage comprises the following raw materials: fruit and vegetable juice enzymolysis fermentation broth, camellia oil, tartary buckwheat polypeptide, a sweetening agent, a stabilizer and distilled water; raw materials of the fruit and vegetable juice enzymolysis fermentation liquor comprise vegetable raw materials, fruit raw materials, enzyme bacteria raw materials and deionized water. The enzyme and bacterium raw materials comprise cellulase, lactobacillus plantarum liquid, helicase, basidiomycetes liquid and neurospora crassa liquid. The raw materials of the fruit and vegetable juice enzymolysis fermentation liquor are subjected to bacterium-enzyme synergistic modification treatment, so that the content of soluble dietary fibers in the fruit and vegetable juice enzymolysis fermentation liquor is remarkably increased, and the fruit and vegetable juice beverage can effectively have the effects of promoting gastrointestinal motility and reducing cholesterol by taking the fruit and vegetable juice enzymolysis fermentation liquor as the raw materials to participate in preparation of the fruit and vegetable juice beverage; in addition, the fruit and vegetable juice enzymolysis fermentation liquor, the camellia oil and the tartary buckwheat polypeptide are combined to achieve a synergistic effect, so that the prepared fruit and vegetable juice beverage has an extremely good cholesterol lowering effect.

The utility model discloses a miniaturized double-frequency wide-beam terminal antenna, which relates to the technical field of mobile terminal antennas and comprises an antenna cap, an antenna housing, a radiator assembly, a feed network module, a radio frequency connector and an antenna base, and the radiator assembly, the feed network module and the radio frequency connector are sequentially distributed inside the antenna housing from top to bottom. According to the miniaturized double-frequency wide-beam terminal antenna, the radiation copper strip is arranged in a gradually-changed spiral form, and when the radiation copper strip is at the same rising angle, the spiral arm with the top open circuit structure has a wider beam width than the spiral arm with the short circuit structure, so that low-elevation electromagnetic signal radiation and reception of the satellite antenna are facilitated; the radiation copper strip is provided with four feeding points, each feeding point is connected with two oscillator arms with different lengths, the circular polarization characteristic and the double-frequency radiation characteristic of the antenna are guaranteed, the two oscillator arms with the same feeding guarantee different rising angles and widths, the isolation degree of double-frequency radiation is improved, the shape of a directional diagram is more regular, and higher radiation gain is achieved.

This invention discloses an APLN monoclonal antibody, its preparation method, and its applications. The APLN monoclonal antibody includes a light chain variable region and a heavy chain variable region. The amino acid sequence of the light chain variable region is shown in SEQ ID No:1, and the amino acid sequence of the heavy chain variable region is shown in SEQ ID No:2. The APLN monoclonal antibody is obtained by immunizing mice with APLN protein, followed by single-cell B-cell sorting and single-cell PCR. Utilizing advanced single-cell B-cell cloning technology combined with single-cell PCR, a novel APLN monoclonal antibody has been developed. The obtained antibody sequence is the result of natural affinity maturation screening by the in vivo immune system, requiring no additional humanization or in vitro affinity maturation steps. It retains the natural high affinity and specificity, and can directly obtain all-natural antibodies with high affinity and high specificity.

The invention discloses a floating foot structure for floating sleep-aiding equipment. The problems that existing equipment is insufficient in buoyancy, large in resistance, high in noise and inflexible in state switching are solved. The floating foot structure comprises a buoyancy tank, a floating foot body sleeved in the buoyancy tank, a guide mechanism between the buoyancy tank and the floating foot body, and a liquid adjusting adaptive part in the floating foot body; the floating foot body is in a big-end-down outline, and both buoyancy and sinking and floating sensitivity are considered. The guide mechanism is arranged to be a plurality of balls, a columnar rod or an anti-collision assembly, so that resistance is reduced and collision is prevented; the liquid adjusting adaptation part is matched with the external assembly to adjust the total mass of the floating foot body, and smooth floating / landing switching of the floating foot body is achieved. The invention further provides equipment applying the structure, and the equipment can be provided with a driving module and a balancing module to optimize the using effect. The structure is matched with a sleeping-aiding bed baby sleeping basket and the like, light, high in strength, capable of being optimized in a scene mode, low in noise, capable of saving energy, stable in movement and outstanding in practicability.

The invention belongs to the technical field of polymer composite materials and functional electrolyte materials, and relates to an interface covalently constructed MOF composite electrolyte film, and a preparation method and application thereof. The method comprises the following steps: preparing a polymer matrix formed by polymerizing imidazole zwitterionic monomers and a metal organic framework material; the preparation method comprises the following steps: sequentially adding a polymer matrix formed by polymerizing imidazole zwitterionic monomers, a metal organic framework material, an ionic liquid and a photoinitiator into a methanol solvent to obtain a precursor suspension; initiating free radical polymerization reaction of acrylate double bonds by the precursor suspension in a photo-initiation or thermal initiation mode to form an initial three-dimensional cross-linked network; and heating the initial three-dimensional cross-linked network to volatilize the methanol solvent, and cooling to room temperature to obtain the interface covalently constructed MOF composite electrolyte film. Through covalent bonding interface engineering design, the problems of poor dispersibility and weak interface bonding of MOF in a polymer matrix are solved. The obtained film has excellent mechanical properties and ionic conductivity.

The present invention discloses a dual-time remote sensing change detection method combining local representation and global modeling, including: obtaining a dual-time remote sensing image pair and dividing the image into sub-images of the same size; establishing a dual backbone network model and pre-training; using the pre-trained dual backbone network model to extract features from the sub-images, and correspondingly obtaining multi-scale local feature maps and global feature maps; performing a dual-branch information cross-fusion operation on the local feature maps and the global feature maps at the same spatial resolution to obtain a joint representation; performing a multi-scale feature map fusion operation on the joint representation of different spatial resolutions to obtain a multi-scale fused feature map; performing difference, upsampling and convolution transformations on the multi-scale fused feature maps obtained from the corresponding sub-images in the image pair in sequence to obtain a change detection map. The method can achieve better target positioning, edge refinement and semantic understanding, ensure detection accuracy and generalization performance, and has a fast data processing speed.

This invention relates to a Cr and Br dual-element doped polyanionic sodium-ion battery cathode material, sodium iron phosphate pyrophosphate, its preparation method, and its application, belonging to the field of sodium-ion battery technology. The general chemical formula of the cathode material of this invention is: Na₄Fe₂O₃. 3‑x Cr x (PO4)2P2O7Br y Where 0 < x < 0.5, 0 < y < 0.7; the precursor wet gel is obtained through a sol-gel process, dried, and then sintered. This invention relates to a Cr- and Br-doped polyanionic sodium-ion battery cathode material, sodium iron phosphate pyrophosphate. The Cr dopant enhances bulk stability, while the gradient distribution of Br forms a fast ion conduction layer on the surface. The two elements together improve the rate capability and cycle performance of the sodium-ion battery. This provides a modification strategy of significant reference value for cathode materials that suffer from low discharge specific capacity and poor cycle performance at high rates due to electron conductance differences.

The invention belongs to the technical field of power systems, and particularly discloses a singular perturbation-reactive decoupling-based order reduction method and device for a network construction type converter. According to the invention, the original state space model is converted; on the basis of a singular perturbation strategy, order reduction processing is carried out according to target variable characteristics; order reduction processing is carried out on a voltage dynamic equation in a reactive power control outer ring, reactive power decoupling is carried out in the mode that a voltage state variable is introduced into an active power control state equation, and a differential equation based on the phase angle relation is established. Through the above mode, aiming at the electromagnetic dynamic process of the fast subsystem, order reduction processing is carried out on the electromagnetic dynamic state variable based on the singular perturbation strategy, order reduction processing is carried out on the voltage dynamic equation in the reactive power control outer loop, high-order dynamic items are eliminated, main dynamic characteristics in the state space are reserved, the model order and the solving complexity are obviously reduced, and the reliability of the system is improved. Therefore, the model order can be systematically reduced on the premise of ensuring key dynamic characteristics and stability analysis precision.

This invention discloses a bivalent anti-Vibrio parahaemolyticus nanobody, its preparation method, a self-sandwich ELISA kit, and a detection method. The aim is to provide a bivalent anti-Vibrio parahaemolyticus nanobody, kit, and detection method with high accuracy, specificity, and sensitivity for detecting Vibrio parahaemolyticus. The amino acid sequence of the bivalent anti-Vibrio parahaemolyticus nanobody is shown in SEQ ID NO.7. This invention belongs to the field of biodetection technology.

The invention provides an intelligent target tracking method based on big data technology deep learning, and relates to the technical field of unmanned aerial vehicle flight position tracking, and the method comprises the steps: reporting data after signal processing, and extracting the state feature information of a target from the echo data after signal processing; a long-short-term memory network is constructed to support matching of motion time sequence information and a scene, and a door structure is introduced into the long-short-term memory network to support selective memorization of long-short-term motion features; building a long-short-term memory network in a data preprocessing mode, supporting a target estimation state at any moment and measurement information input at the next moment, and outputting parameters according to a mapping relation; the technical key points are as follows: state feature information of a target is extracted from echo data after signal processing, all data are imported into a long-short-term memory network, the network has the capability of processing long-term dependence and short-term dependence in combination with processing of a gate structure, and finally the tracking precision of the weak and small target in a complex scene is improved.

The invention relates to a preparation method and application of M1 type macrophages, and the method comprises the following steps: adding activated mouse lymphocytes into a mouse macrophage culture system for co-culture to obtain the M1 type macrophages. The invention explores the identification of CD3 / CD28 activated lymphocytes, the influence of the activated lymphocytes on the morphology of macrophages and the influence of the activated lymphocytes on macrophage subtypes, and provides a brand-new method for obtaining the mouse M1 type macrophages. According to the invention, the problem that the polarization of the macrophage cannot be completely induced by singly using the recombinant IFN-gamma to stimulate the macrophage is solved. The invention provides a novel in-vitro mouse M1 type macrophage model for experimental research, M1 type macrophages induced by activated lymphocytes can better simulate an in-vivo immunoreaction process, a required cell state is obtained by utilizing co-culture of two cells in vivo, and compared with dependence on exogenous addition of viruses or compounds, the in-vitro mouse M1 type macrophage model provided by the invention has the advantages that the in-vitro immunoreaction process can be better simulated by the M1 type macrophages induced by the activated lymphocytes; according to the invention, many potential risks and bad places can be effectively avoided.

The invention relates to a multi-target detection method, which comprises the following steps of: based on multidirectional anisotropic filtering, effectively decoupling track main body texture and environmental background noise, and remarkably improving the feature expression capability and the signal-to-noise ratio in a low-contrast and complex texture environment; the fracture area of the track caused under the severe working conditions of partial shielding, light reflection and the like is positioned and repaired, and the connectivity and integrity of the output track on the topological level are guaranteed; by constructing a feasible region mask surrounding an orbit, spatial semantic ambiguity caused by a monocular vision perspective effect is eliminated, it is ensured that foreign matter alarm is strictly limited in a physical dangerous region, and the false alarm rate is greatly reduced. Through simultaneous extraction of the railway track topological structure and troubleshooting of the foreign matter risk in the track area, the detection precision under the complex working condition is significantly improved.

The invention discloses a primer probe set and a kit for mycoplasma pneumoniae nucleic acid detection and application of the primer probe set and the kit in a method for directly detecting mycoplasma pneumoniae without extraction, and belongs to the technical field of molecular biology detection. Comprising an upstream primer MP-F as shown in SEQ ID NO: 1, a downstream primer MP-R as shown in SEQ ID NO: 2 and a probe MP-P as shown in SEQ ID NO: 3. The mycoplasma pneumoniae type I and the mycoplasma pneumoniae type II can be efficiently and specifically detected, a fluorescent quantitative PCR detection method can be adopted, PCR detection can be directly carried out without nucleic acid extraction, the detection cost is reduced, the test operation is simplified, the detection time is shortened, meanwhile, high specificity and accuracy are guaranteed, the sensitivity is improved, and the detection cost is reduced. The kit can be used for detecting mycoplasma pneumoniae nucleic acid in a human throat swab.

The utility model discloses a blocking and sealing structure of a slewing bearing, and belongs to the technical field of slewing bearings. A plug with an end face annular groove is arranged on one side of the outer ring, an O-shaped ring is arranged in the annular groove of the plug, the plug is connected with the outer ring through a taper pin, the inner ring is arranged on the inner side face of the outer ring, a roller path is formed in the middle of the outer ring and the middle of the inner ring, a rolling body is arranged in the roller path, and a sealing strip is arranged between the outer ring and the inner ring. The end face of the inner ring is provided with an oil hole, the oil hole is communicated with the roller path, the end opening of the oil hole is provided with an oil cup, and the oil cup is fixedly inserted into one side of the inner ring. The O-shaped ring is additionally arranged on the end face of the plug, and certain axial pressure is formed on the O-shaped ring after the taper pin is assembled, so that when the pressure of internal grease rises, a stronger sealing effect is obtained; the main body is simple in structure and convenient to use and has great popularization and application values.

The invention provides a capture primer group for HIV-1 whole genome sequencing, a kit, a capture method and application thereof, 31 pairs of imbricate amplification primers are designed according to the imbricate principle for the whole genome of HIV-1, and the 31 pairs of primers are divided into two primer pools, so that HIV-1 whole genome reverse transcription and capture amplification can be performed on RNA extracted from plasma, and the detection sensitivity is high. The obtained DNA product is subjected to a second-generation or third-generation whole genome sequencing experiment to obtain the whole gene of the HIV-1, and the method has the advantages of convenience and rapidness, and is convenient for meeting current molecular network propagation mode analysis, virus recombination variation and traceability research.

The present invention discloses a design method for primers and probes for amplifying low-concentration mutant target sequences. First, the mutation position (mutation point, i.e., position 0) of the target sequence to be amplified is determined; then, a 15-25bp nucleic acid fragment including the 0 base is selected in the negative direction of the mutation point as a forward primer, and a 12-25bp nucleic acid sequence is selected from the ‑1 base or the 0 base in the positive direction of the mutation point as the probe sequence of the amplification system; finally, a reverse primer is designed according to a conventional method at a suitable position downstream of the 3' direction of the probe sequence. The primers and probes designed according to the method of the present invention can effectively (high specificity and high efficiency) amplify the target fragment under the background of a higher content of wild-type template, especially for point mutations, deletion mutations, and insertion mutations in gene mutations, etc., combined with fluorescent real-time PCR technology, can effectively solve the difficulty of insufficient sensitivity in current clinical tumor detection and drug sensitivity detection.

The present invention relates to the field of in vitro immunodiagnosis and detection, and in particular to an IgG antibody, an IgM antibody and a method. The present invention provides an IgG antibody that specifically binds to a coxsackie virus, characterized in that the amino acid sequences of the complementary determining regions CDRH1-CDRH3 of the heavy chain variable region of the IgG antibody are shown in SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3, respectively; the amino acid sequences of the complementary determining regions CDRL1-CDRL3 of the light chain variable region are shown in SEQ ID NO: 4, SEQ ID NO: 5 and SEQ ID NO: 6, respectively. The IgG antibody provided by the present invention not only has good specificity and can specifically identify coxsackie viruses, but also has a high level of sensitivity and stability.

This invention provides a Cr2Se3 single crystal and its preparation method, relating to the field of crystal materials technology. The Cr2Se3 single crystal preparation method, from raw material proportioning and powder preparation to single crystal growth, involves coordinated steps to suppress impurity introduction and impurity phase formation, while simultaneously promoting ordered atomic arrangement and eliminating lattice vacancies and dislocations, resulting in a Cr2Se3 single crystal with good single-phase properties, high crystallinity, and extremely low defect density. The high-purity, low-defect single crystal structure ensures its semiconductor transport properties and antiferromagnetic order; it also eliminates interference from impurity scattering and lattice distortion, optimizing the magnetic field's control over the magnetic moments of Cr atoms, enabling the single crystal to exhibit a strong CMR effect approaching -100%. Furthermore, the coordinated steps ensure consistency in size, phase composition, and structural integrity of the prepared Cr2Se3 single crystal, significantly improving the success rate, process controllability, and repeatability.

The invention provides a Dermatophagoides pteronyssinus recombinant antigen and application thereof in an allergen detection test strip, Derf1, Derf2 and Derf3 recombinant antigens are constructed, amino acid sequences of the Derf1, Derf2 and Derf3 recombinant antigens are optimally designed, especially D58P, S106D and G231C sites in Derf3 are mutated, so that the test strip capable of detecting the three recombinant antigens at the same time is prepared, and the test strip can be used for detecting the three recombinant antigens at the same time. The problems of wrong detection result, false positive and the like of Derf3 during simultaneous detection are effectively solved, simultaneous detection of the specific IgE antibodies of the three dermatophagoides farinae allergens in the test strip is realized, the problem of cross influence is avoided, direct allergen type judgment is given for patients allergic to dermatophagoides farinae before desensitization treatment, and the sensitivity of the patients is improved. And more effective desensitization treatment can be conveniently carried out on symptoms.

The present invention relates to the technical field of power semiconductor modules, and proposes a low-inductance packaging structure of a power semiconductor module, comprising a substrate and a power semiconductor module unit arranged on the substrate, the power semiconductor module unit is used to form a dual-switch circuit structure capable of reducing inductance, the power semiconductor module unit comprises a plurality of lining plates arranged at intervals on the substrate, adjacent lining plates are connected by a first bonding wire, two connected lining plates are connected with a power terminal group extending out of an outer tube shell, the power terminal group comprises two sub-power terminals which are partially overlapped but not in contact with each other, and are used to generate electromagnetic coupling when the two sub-power terminals conduct currents in different directions to reduce inductance; a low-inductance packaging method of a power semiconductor module is also proposed, which is used to manufacture the low-inductance packaging structure of a power semiconductor module proposed by the present invention. The present invention has the advantages of high reliability, high power density and low inductance.

This invention relates to a negative electrode sheet, its preparation method, and a battery. The negative electrode sheet includes a current collector and an active material layer disposed on at least one surface of the current collector. A coating is disposed on the surface of the active material layer away from the current collector. The coating material includes modified lithium titanate. In this invention, a coating containing modified lithium titanate is disposed on the surface of the active material, which can effectively improve the safety of the battery cell during overcharging. The modified lithium titanate coating not only improves the chemical stability of the negative electrode interface and reduces internal heat generation during overcharging, but also effectively suppresses the excessive lithium plating caused by overcharging by the structural stability of the modified lithium titanate coating, thereby inhibiting lithium dendrite growth and greatly reducing short circuits caused by microperforations in the separator.

The invention provides a semiconductor device, a preparation method thereof and a chip, relates to the technical field of semiconductors, and aims to reduce the process difficulty. The preparation method of the semiconductor device comprises the following steps: forming a bottom electrode on a substrate; forming a dielectric layer by adopting a sputtering process, wherein the dielectric layer is positioned on one side, far away from the substrate, of the bottom electrode; a top electrode is formed and located on the side, away from the substrate, of the dielectric layer; wherein in the process of forming the dielectric layer, the substrate is heated, and the heating temperature ranges from 50 DEG C to 400 DEG C; moreover, in the process of forming the dielectric layer, direct current bias voltage is applied to the substrate, the change range of the direct current bias voltage is-500V to + 150V, or radio frequency bias power is applied to the substrate, and the change range of the radio frequency bias power is 50w to 400w. The semiconductor device can be applied to a memory chip or a sensor chip, the memory chip can be a nonvolatile memory, and the sensor chip can be a micro electro mechanical system pressure sensor.

The invention discloses a mycoplasma pneumoniae gene detection kit based on a silicon-based micro-fluidic chip and a detection method thereof, and the kit provided by the invention comprises a rapid hot start DNA polymerase, a PCR reaction mixed solution, a positive reference substance and a negative reference substance, and also comprises a PCR reaction system which takes a mycoplasma pneumoniae repMp1 gene as a target gene, the following primers are designed by a PCR (Polymerase Chain Reaction) fluorescent probe method: an upstream primer F, a downstream primer R and a detection probe P, and the nucleotide sequences of the upstream primer F, the downstream primer R and the detection probe P are shown as SEQ ID NO.1-3. The mycoplasma pneumoniae gene detection method provided by the invention comprises the steps of extraction of pathogen DNA (Deoxyribonucleic Acid) and PCR (Polymerase Chain Reaction) amplification detection. According to the invention, the mycoplasma pneumoniae gene detection kit which is more comprehensive in detection effect, high in specificity, good in sensitivity, low in omission ratio, convenient, simple and easy to operate is provided.

The invention relates to an iNKT-TCR molecule of a targeted lipid antigen and CD1d compound, a TCR-T cell and preparation and application, and belongs to the technical field of genetic engineering and immunity. According to the invention, the specific iNKT amplification technology is utilized to amplify and sort the iNKT cells, and the TCR enriched with the iNKT cells is obtained by sequencing analysis. According to the research, iNKT cells are amplified through an iNKT cell specific activator, high-purity iNKT cells are obtained through magnetic bead sorting, TCRs enriched with the iNKT cells are accurately screened out according to sequencing analysis, and the accuracy and specificity of the TCRs are guaranteed. The NKT38 provided by the invention has a higher killing effect on tumor cells expressing CD1d and presenting endogenous lipid antigens, and has very strong specificity: in-vitro experiments show that the iNKT-TCR transfected NKT38 cells have a stronger killing effect on tumor cell lines expressing CD1d and presenting endogenous lipid antigens, and prompt that the iNKT-TCR transfected NKT38 cells have a specific killing effect on tumors.

The invention discloses a PfAgo nuclease-based single-channel rapid detection method and kit for five drug-resistant genes of carbapenemase-producing enterobacteriaceae. The method comprises the following steps: extracting nucleic acid to obtain nucleic acid to be detected; the method comprises the following steps: simultaneously amplifying to-be-detected sites of three drug-resistant genes by using three groups of different PCR (Polymerase Chain Reaction) primers to obtain nucleic acid products, namely a KPC type gene amplification product, an NDM type gene amplification product and a VIM type gene amplification product; preparing a PfAgo reaction mixed solution, adding 20 [mu] L of the nucleic acid amplification product into the PfAgo reaction mixed solution, incubating at 95 DEG C for 30 min, and reading fluorescence to obtain a detection result. According to the invention, the PfAgo derived from the pyrococcus violaceus is used for detection, and the method has the advantages of fast detection speed, high accuracy and low cost.

The application relates to the technical field of agricultural product detection, and discloses a detection method for maneb-zinc residues in vegetables, which comprises the following steps: extract solution preparation, sample pretreatment, chelation and extraction, salting-out and purification, concentration and redissolution, and detection analysis; in the pretreatment stage, a dimethyl sulfate acetonitrile solution with a specific concentration is used as an extraction solution, and under the synergistic action of a first salt bag containing L-cysteine hydrochloride and a second salt bag containing ethylenediaminetetraacetic acid disodium salt, the sample is extracted through vortex oscillation; the operation combines the derivatization of the target and the solvent extraction process, improves the efficiency and completeness of the derivatization reaction, reduces the decomposition and loss risk of the target in the complex pretreatment process, solves the problems of incomplete derivatization and instability of the target in the traditional method, and lays a reliable foundation for subsequent quantitative analysis.