The invention discloses fishbone bioactive polypeptide 
calcium powder and a preparation method, belonging to the field of 
functional food or food additives. The preparation method comprises the following steps: freezing fishbone, coarse 
grinding, fine 
grinding, adding water, stirring, adding a compound 
protease to hydrolyze, centrifugating, and respectively collecting the supernatant and the precipitate; ultrafiltering the supernatant by a filter membrane, concentrating the filtrate, and freeze-
drying to obtain the fishbone 
protein polypeptide 
powder; washing the precipitate, 
drying and pulverizing; activating by using 
citric acid, and 
drying to obtain the soluble 
calcium; and mixing the fishbone 
protein polypeptide 
powder and the soluble 
calcium to obtain the fishbone bioactive polypeptide calcium powder. The fishbone bioactive polypeptide calcium powder enables the biological absorptivity of calcium to reach above 65%, and meanwhile, maintains the functional characteristics of polypeptide. 
Hydrolysis is carried out by using the food-grade 
protease, and thus, the fishbone bioactive polypeptide calcium powder has the advantages of high safety and no 
side effect. The method of the invention has the advantages of simple and feasible realization, and low cost. The fishbone bioactive polypeptide calcium powder can be used as a 
food additive or directly eaten as nutritional food.