267 results about "Throat swab" patented technology

The invention discloses a throat swab automatic sampling system. The throat swab automatic sampling system comprises a to-be-tested person identity information automatic checking and acquisition prompting unit, a to-be-tested person head positioning unit, a navigation positioning unit, a sample acquisition execution unit, a throat swab automatic loading and unloading unit and a remote monitoring unit which are arranged on a working platform; the navigation positioning unit enters the oral cavity of a to-be-detected person along with the tail end of the sample collection execution unit and is used for collecting a throat image and determining depth information between a depth sensor and the throat of the to-be-detected person; and the sample collection execution unit comprises a three-degree-of-freedom guide rail type device and a one-degree-of-freedom end execution mechanism. In a throat swab collecting process, real-time information of all the units is in communication with the remotemonitoring unit, and medical staff conduct real-time monitoring on the collected person and all the units through a computer in a remote safety room, so that measures are taken in time to guarantee safety and effectiveness of the whole process when an accident occurs. According to the system, full automation of the throat swab collection process is achieved.

The invention relates to a mobile throat swab robot system, and specifically relates to a mobile throat swab sampling robot, a supporting system and operation software. The sampling robot is mainly used for the throat swab sampling operation of mobile detection points, so that doctors can achieve process interference and sampling position correction. The whole system can guarantee the separation of doctors and patients during throat swab sampling, can complete operations such as contact judgment, sampling quality judgment, throat swab sampling, throat swab head cutting and transferring and hand disinfection under fully automatic or partial manual intervention and can synchronously record data including but not limited to collection strength and positions, so that the influences of highly infectious diseases represented by the novel coronavirus on medical staff can be greatly reduced, physical and mental burden of the medical staff can be relieved, and medical resources such as protective suits and gloves can be saved; and the sampling robot can be directly connected to a mobile platform, so that the rapid moving of throat swab collection place construction and the collection and sampling of throat swabs can be realized.

The invention relates to a nucleic acid detection kit for novel coronavirus COVID-19. The kit comprises a first primer pair and a first probe which correspond to Cov-n, and a second primer pair and asecond probe which correspond to Cov-ORF1ab. The kit disclosed by the invention can be used for simultaneously carry out multiple-gene and multiple-site detection on the novel coronavirus, so that thedetection accuracy is improved; and in addition, when the multiple fluorescence PCR reaction is carried out, novel coronavirus detection information can be provided through one-time detection within70 minutes, so that the diagnosis time of the novel coronavirus COVID-19 is shortened. The kit can be used for in vitro qualitatively detecting throat swabs, sputum and bronchoalveolar lavage fluid samples of suspected pneumonia cases infected by the novel coronavirus, suspected aggregated case patients, and other diagnosis people who are required to perform novel coronavirus infection diagnosis or identification.

The invention discloses a primer and a probe for quantitative determination of klebsiella pneumonia, and an application of the primer and the probe. Nucleotide sequences of the primer are a sequence 3 and a sequence 4 in a sequence table; a nucleotide sequence of the probe is a sequence 5 in the sequence table. The primer and the probe have the beneficial effects that the specific primer and probe sequences of the klebsiella pneumonia disclosed by the invention can be applied to qualitative and quantitative detection of the klebsiella pneumonia, the target of accurately and quantitatively determining the deoxyribonucleic acid (DNA) content of the klebsiella pneumonia in a specimen to be detected can be achieved by extracting the DNA in the sample to be detected and combining with a real-time fluorescence quantification polymerase chain reaction (PCR) detection technology, and the primer and the probe can be applied to food detection, scientific research and clinical diagnosis and used for carrying out qualitative and quantitative analysis on the klebsiella pneumonia DNA in samples such as a food sample, a suffer throat swab, nasopharyngeal secretion, people sputum specimens and blood samples. Thus, the primer and the probe have an important significance on judgment of klebsiella pneumonia infection, evaluation of treatment effectiveness and dynamic observation of the state of an illness, and simultaneously play an important role in the field of detection of clinical medicine.

The invention discloses a closed type throat swab sampling device. According to the device, the upper end of a handle is connected with a display screen through a connecting shaft, the lower part of the handle is connected with a long plate, the top end of the long plate is connected with a tongue depressor, and the long plate is provided with a sampling cavity and a camera; a fixed valve is arranged at the joint of the long plate and the handle; and a sealing cover is fixedly or movably sleeved on the long plate, and an elastic band is arranged at the middle hole to be tightly bonded with thelong plate. The invention has the advantages that: the device can be used to solve the problem of how to safely and correctly collect throat swab specimens by medical staff for infectious respiratorydiseases, particularly for patients with corona virus disease 2019 at present. Oral and nasal parts of the patient are properly covered in a closed manner, thus avoiding an extremely high risk of infection since the medical staff directly face respiratory tract pathogenic bacteria when collecting throat swab specimens of patients; and a real-time image in the oral cavity is displayed in vitro byleading-in the camera with a light source to the oral cavity, and the medical staff can correctly and quickly acquire an ideal throat swab specimen under the guidance of the real-time image.

The invention discloses a throat swab double-arm sampling robot and sampling method based on machine vision guidance. A robot system utilizes an industrial depth camera for acquiring cloud informationof an RGB-D depth point, oral space environment is recognized and processed, a deep learning algorithm is adopted, three-dimensional coordinates of a tongue tip and a throat deep part are determinedto serve as expected coordinates of two arms, collaborative motion planning of moving of the two arms is completed on the basis of a moveit function package, a robot control system performs wireless communication with ROS on the basis of ROS system development through human-computer interaction control, and remote control and real-time monitoring functions are realized. According to the robot, medical staff can be prevented from making direct contact with infectious substances in a body of a patient, completion of a complex throat swab sampling process by the double-arm robot is monitored, high execution precision and success rate are guaranteed, and accidental risks such as an injury occurring in the sampling process of the patient are avoided.

The invention provides a throat swab acquisition method based on image identification and positioning. The method comprises the following steps: S1, shooting at an acquisition far end to obtain a far end acquisition image, identifying a circular contour of a mouthpiece in the far end acquisition image, and obtaining pixel coordinates of a circle center of the mouthpiece; S2, correcting a position of a throat swab acquisition mechanism, enabling the circle center pixel coordinates of the mouthpiece to coincide with pre-calibrated circle center pixel standard coordinates of the mouthpiece, and carrying out coarse positioning; S3, shooting at an acquisition near end to obtain a near-end acquisition image, and identifying uvula center pixel coordinates in the near-end acquisition image; and S4, calculating pixel coordinates of a replacement point of the acquisition area according to the uvula center pixel coordinates, and correcting the position of the throat swab acquisition mechanism, enabling pre-calibrated light spot circle center pixel coordinates to coincide with the pixel coordinates of the replacement point, and carrying out accurate positioning. The identification and positioning precision of the acquisition area is improved through two-step positioning, and the throat swab acquisition mechanism can be accurately guided to move to the position of the acquisition area.

The invention provides a preserving fluid for biological sample nucleic acid detection and an application thereof. The preserving fluid is composed of a nuclease inhibitor, a surfactant, a chelating agent and a buffer solution. The preserving fluid is mixed with a solid sample or a liquid sample to denature proteins, inactivate pathogens, and inactivate DNA and RNA nuclease. The pathogens are inactivated, so the safety of a potential high-infectivity clinical sample is greatly improved, and good protection is formed for surrounding personnel and downstream operating personnel of the sample; the DNA and RNA nuclease is inactivated, so that the content and integrity of DNA and RNA in the sample are kept in a sampling state to the maximum extent, and the strict requirements on the aspects oftemperature, time and the like of clinical sample transportation and storage are reduced. The preserving fluid can be used for clinical high-infectivity nucleic acid detection of biological samples, and the biological samples comprise throat swabs, body fluids, lavage fluids, whole blood, whole blood cells, serum or plasma.

The present invention relates to a new strain of coronavirus and its application. Said invention utilizes the throat-swabbed cell colture of SARS patient to separate out a new type coronavirus, called by name of SARS coronavirus Fudan I strain. By infecting Vero E 6 cell and patient's serum the indirect FA display is positive, after the influenza virus A and B, RS virus and pathogens of pneumococcus, etc. are excluded, the tests show that said separated coronavirus is a variant strain of new coronavirus. Said strain can be used for providing basis for preparing SARS diagnosis reagent and researchnig and producing medicine for resisting and curing SARS disease.

The invention provides a throat swab sampling system and a sampling method. The throat swab sampling system and the sampling method have the advantages that the oral cavity of a patient to be sampled is positioned through a camera and a laser range finder in a sampling device, so that the sampling accuracy of a throat swab is further improved, the accurate positioning of the throat swab sampling is guaranteed, and the parallax influence is reduced; a pressure sensor is arranged in the sampling device, when the throat swab is in contact with a sampling part, a pressure value fed back by the pressure sensor changes, and the device stops moving; a buffer mechanism is arranged in a sampling head device, so that the contact is relatively soft, and the sampled patient does not have obvious pain; and under the isolation condition, medical staff remotely controls the throat swab sampling system and moves the throat swab sampling system to the front of the patient to sample the oral cavity of the patient, so that the infection caused by direct contact between the medical staff and the patient in the sampling process can be avoided, the patient can be safely subjected to deep examination, the sampling safety of the throat swab is improved, and the safety of the medical staff is guaranteed.

The invention provides a herpes virus EBV (Epstein-Barr Virus) detection kit. The kit comprises a nucleic acid releasing agent and PCR (polymerase chain reaction) reaction solution, wherein the nucleic acid releasing agent comprises 0.01-0.5 mM/L of surfactin, 20-300 mM/L of potassium chloride, 0.01-2% of sodium dodecyl sulphate and 0.05-1% of ethanol; and the PCR reaction solution comprises an upstream primer and a downstream primer used for target polynucleotide amplification, and a probe used for target polynucleotide detection. The detection result of the method for releasing nucleic acid by the nucleic acid releasing agent in the kit disclosed by the invention has no obvious difference with the detection result of a boiling method, a strong protein denaturing agent is used during nucleic acid extraction in the kit disclosed by the invention for rapidly breaking the coat protein structure of a pathogen and releasing the nucleic acid of the pathogen, and release and extraction for DNA (deoxyribonucleic acid) can be rapidly finished without heating; the sensitivity of the EBV detection of the kit disclosed by the invention can achieve 400 copies/ml, and the quantitative linear range is 400-4.00E+09 copies/ml; by applying the kit, rapid and accurate detection can be performed on EBV-DNA in the unknown samples of blood plasma, throat swab, peripheral blood and the like, and reliable experimental basis is provided for diagnosing EBV infection.

The invention relates to the technical field of throat swab sampling, and discloses an integral throat swab self-service sampling device. The device comprises a machine body, wherein a pre-processingbin for placing a sampling tool, a sampling unit and a post-processing bin are arranged in the machine body; the sampling unit is provided with a sensing area, a user camera and a scanning head; a capscrewing structure for screwing off or covering a tube cap of a sampling tube, a shearing structure for shearing a sampled throat swab on the sampling tool and placing the throat swab in the samplingtube, and a storage manipulator for placing the sampling tube for placing the throat swab in a sample storage box are arranged in the post-processing bin; a user obtains the sampling tool in the pre-processing bin, and information of the user corresponds to information of the sampling tool; the tube cap of the sampling tube is screwed off through the cap screwing structure; the throat swab is sheared through the shearing structure and placed in the sampling tube; the tube cap is covered on the sampling tube; and the storage manipulator places the sampling tube in the sample storage box. The probability of virus infection of workers is reduced in the whole process, potential safety hazards are avoided, the efficiency is high, and rapid nucleic acid sampling of large-group users is met.

The invention discloses a virus nucleic acid extraction kit based on nano magnetic beads. The lysis liquid comprises guanidine thiocyanate/guanidine hydrochloride, a nonionic surfactant, tris (hydroxymethyl) aminomethane hydrochloride and ethylenediamine tetraacetic acid, and the nonionic surfactant comprises one or more of triton X-100, Tween 20 and ethyl phenyl polyethylene glycol. The binding liquid is isopropanol, the volume ratio of isopropanol to pyrolysis is 1: 1, and the magnetic beads are silicon oxide hydroxyl nano magnetic beads. According to the kit, a nano magnetic bead extractionmethod is adopted, isopropanol is used as a binding liquid, making sample splitting, binding of nucleic acid and magnetic beads to be done in one step. The steps are simple, convenient and easy to implement, and the automatic extraction process of virus nucleic acid can be completed quickly at high flux within 9 min. The kit disclosed by the invention can be used for extracting RNA and DNA of viruses in sample, such as plasma, serum, nasopharynx swabs, urine, secretion liquid, virus concentrate, cell culture liquid supernatant and virus preservation liquid.

The invention discloses an automatic throat swab collection protection device. The automatic throat swab collection protection device comprises a cabin, a door arranged on the cabin, a seat arranged in the cabin to allow a patient to sit and a manipulator arranged in the cabin to collect a throat swab, wherein air holes are formed in the door, and an exhaust and disinfection device is arranged outside the cabin and communicates with the interior of the cabin through a ventilating pipe. In the automatic throat swab collection protection device, the cabin as a relatively closed environment provides physical isolation for the patient and a collection medical worker, so that spray or aerosol carrying viruses can be prevented from spreading from the mouth of the patient and diffusing to the outer environment when the patient is subjected to throat swab collection, and the infection risk of the medical worker is effectively reduced.

The invention discloses a novel coronavirus 2019-nCoV nucleic acid kit and a virus nucleic acid acquisition method. The method comprises the following steps: according to novel coronavirus 2019-nCoV ORF1ab and an N gene as amplification target areas, designing specific primers and fluorescent probes for detecting novel coronavirus 2019-nCoV nucleic acid in samples; and performing Digital PCR (polymerase chain reaction) absolute quantification on 2019-nCoV nucleic acid RNA (ribonucleic acid) by using a one-step method by using a prepared Super premix reaction liquid A together with Super premixreaction liquids B and C, and performing Digital PCR detection on RNA of a diluted positive virus sample. The kit and the method have the beneficial effects that by using the novel virus nucleic acidacquisition method, and together with a Digital PCR probe method, the nucleic acid positive detection rate of infection of the novel coronavirus 2019-nCoV can be increased, the process is ensured tobe specified and standardized, the influence of artificial non-standard operation upon detection results can be reduced, result reliability can be ensured, and meanwhile, false negative results causedby detection of sputum and throat swab sampling together with real-time fluorescent PCR methods can be avoided.

The invention proposes an automatic throat swab sampling system, which comprises an automatic to-be-tested person identity information checking and collection prompting unit, a to-be-tested person head positioning unit, a navigation positioning unit which enters the oral cavity of a to-be-detected person along with the tail end of the sample collection execution unit for collecting a throat image and determining depth information between a depth sensor and the throat of the to-be-detected person, a sample collection execution unit including a three-degree-of-freedom guide rail type device and a one-degree-of-freedom end execution mechanism, an automatic throat swab loading and unloading unit and a remote monitoring unit which are arranged on a working platform. In a throat swab collecting process, the real-time information of all units communicates with the remote monitoring unit, and medical staff monitors the collected person and all the units in real time through a computer in a remote safety room, so that measures can be taken timely to guarantee the safety and effectiveness of the whole process when an accident occurs. The system realizes the full-automatic throat swab collection process.

The invention discloses a novel coronavirus remote throat swab sampling and automatic specimen collecting unit. The novel coronavirus remote throat swab sampling and automatic specimen collecting unit comprises a frame, a disinfection long cotton swab feeding device, a first rotating disc, a second rotating disc, a first electric sliding rail, a second driving motor, first clamping calipers, a third driving motor, second clamping calipers, a throat swab sampling mechanical arm, a disinfection long cotton swab packaging bag unsealing and cutting mechanism, a throat swab specimen cotton swab conveying frame and a second electric sliding rail, throat swab specimen cotton swab steering calipers, a throat swab sampling head cutting mechanism, a specimen collecting device, identity card scanning equipment and a loudspeaker. The novel coronavirus remote throat swab sampling and automatic specimen collecting unit has the beneficial effects that throat swab sampling and specimen collection can be remotely and automatically carried out, the risk that medical personnel are infected in the nucleic acid detection process is reduced, the working comfort is improved, heavy protective clothing does not need to be worn, and meanwhile, the waste of human resources is reduced.

The invention discloses a flexible throat swab sampling device. The flexible throat swab sampling device comprises a flexible throat swab body and a feeding device, wherein an airway is arranged in the flexible throat swab body, and the flexible throat swab body is arranged to be sleeved with a sampling part; the feeding device is connected with the flexible throat swab body; the airway is arranged for gas to enter; the flexible throat swab body is arranged to deform under the action of the gas entering the airway, so that the sampling part located in the mouth of a user scrapes the inner wallof the mouth of the user; and the feeding device is arranged to drive the flexible throat swab body to move. The flexible throat swab body of the flexible throat swab sampling device is made of a soft material, and can be bent as required; on the one hand, through friendly interaction with human bodies, symptoms such as nausea and cough caused by discomfort of patients are reduced, and viruses are prevented from spit and infection.

The invention discloses a nucleic acid detection cassette based on magnetic bead transfer and valve controlled pipetting and a detection method thereof, a cassette body is divided into a nucleic acidextraction area and an amplification detection area, the amplification detection area is provided with a rotary valve tube, a PCR tube, a gas pump interface, a gas channel and a liquid channel, the gas channel respectively extends from the rotary valve tube to a liquid mixing tube and the gas pump interface. The liquid channel respectively extends from the rotary valve tube to a elution tube, theliquid mixing tube and the PCR tube; and a rotary valve for controlling the opening and closing of the gas channel and the liquid channel is arranged in the rotary valve tube. According to the presentinvention, the clinical collection throat swab without any treatment can be directly added, the nucleic acid extraction is achieved by externally driving a magnetic rod and a magnetic rod sleeve, theopening and the closing of the related liquid channel and the gas channel are achieved by controlling the rotary valve, the directional transfer of the liquid can be achieved under the effect of theexternal pump, and finally the real-time fluorescence PCR amplification detection is performed in the PCR tube, so that sample input and result output can be simply and efficiently realized.

The invention relates to a method for detecting component information of a throat swab sample by adopting surface enhanced micro-Raman spectroscopy. The method comprises preparation of a surface enhancement reagent, treatment of the throat swab sample, measurement of a surface enhanced Raman spectrum of the sample and processing and analysis of Raman spectrum data. According to the technical scheme, the method comprises the following steps: preparing the enhancement reagent for enhancing the Raman spectrum of the sample; smearing the throat swab sample on a smooth and clean pure aluminum sheet, dripping the surface enhancement reagent, and naturally air-drying the pure aluminum sheet at room temperature to prepare a throat swab-surface enhanced Raman test sheet; performing micro-Raman spectroscopic test on the throat swab-surface enhanced Raman test sheet to acquire the surface enhanced micro-Raman spectrum of the throat swab sample, and analyzing biochemical component information and features of the throat swab sample by virtue of spectral features. The method has the advantages of short detection time, low cost, convenience and rapidness in process and the like, and rich biochemical component information of the throat swab sample can be obtained, so that a rapid and effective analysis method can be provided for biochemical features and changes of intracavity mucous tissues.

The invention provides a respiratory virus sample collection and detection integrated device. A detection card is arranged on a wearing device, droplets are collected by protective equipment to be used as a sample for direct detection, protection is integrated with sample collection and detection, and viruses in the droplet sample can be automatically detected; manual throat swab sampling and detection operation is not needed, so on the one hand, a large amount of manpower and material resources can be saved, and meanwhile, the detection speed is remarkably increased; on the other hand, directcontact between medical staff and the droplets of the patient is effectively reduced, the mask can also be used as a common mask for isolating the droplets in the sampling detection process, and theinfection risk in the sampling process is greatly reduced. The device is suitable for rapid and efficient diagnosis of a large number of to-be-detected personnel, is especially suitable for rapid screening of a large number of suspected cases and closely contacted persons of definite cases, and can effectively relieve the pressure of detection and diagnosis work.

The invention belongs to the technical field of nucleic acid test sampling, and provides a flexible operating head, a rigid base and a facility for nucleic acid test sampling. On the basis of the principle that a cavity formed inside a flexible material can deform in the air inflation and deflation and/or pumping process, the flexible operating head with an air cavity structure is obtained and used for allowing a throat swab to be installed and controlling the throat swab to conduct sampling operation, and therefore a medical worker does not need to hold the throat swab to conduct operation byhimself in the sampling process. The flexible operating head can cooperate with the rigid base and a mechanical arm to be used, and therefore the facility which can allow the medical worker to conduct long-distance sampling operation is obtained, the medical worker is prevented from touching a patient in a short-distance mode, and the infection risk is reduced. In addition, the replacement of thedisposable flexible operating head can be automatically accomplished on the basis of the magnetic attraction connection principle, the process is safe and effective, and the cost is low.

The invention provides full-automatic self-service throat swab collection equipment for nucleic acid detection. The full-automatic self-service throat swab collection equipment comprises a human body limiting assembly, a collection driving assembly, a cotton swab frame assembly, a sample storage tube and a control device, wherein a height limiting device of the human body limiting assembly is arranged on the bottom surface and is used for adjusting the height of a human body sitting posture, and the head limiting device is arranged above the height limiting device and is used for adjusting a pitching angle; a double-linear-guide-rail device of the collection driving assembly is obliquely installed on the front side of the head limiting device, a mechanical arm is installed on the double-linear-guide-rail device, and a clamp is installed at the end of the mechanical arm; the cotton swab frame assembly is arranged below the double-linear-guide-rail device and located on the front side of the head limiting device, and the clamp clamps cotton swabs; the sample storage tube is arranged on the front side of the head limiting device and is arranged below the mechanical arm; and the control device is electrically connected with the human body limiting assembly, the collection driving assembly and the cotton swab frame assembly. According to the technical scheme, the problems that in the prior art, when a throat swab is used for sampling, the infection risk of sampling personnel is high, and examined personnel are prone to cross infection are effectively solved.