Method for detecting biochemical components of throat swab sample by adopting surface enhanced micro-Raman spectroscopy
A surface-enhanced Raman and surface-enhanced technology, which is applied in Raman scattering, material analysis through optical means, and measurement devices, can solve problems such as weak Raman spectrum signals, achieve good economic and social benefits, and improve feasibility Sex, process convenient and quick effect
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Embodiment 1
[0028] Example 1 Surface Enhanced Raman Spectrum Detection of Nasopharyngeal and Pharyngeal Swab Specimens
[0029] (1) Preparation of surface enhancing reagent:
[0030] 1) Take 5mL of hydroxylamine hydrochloride solution with a concentration of 0.06mol / L and add it to 4.5mL of sodium hydroxide solution with a concentration of 0.1mol / L; quickly add 90mL of nitric acid with a concentration of 0.0011mol / L to the prepared mixed solution In the silver solution, stir rapidly until the solution is uniform milky gray, which is SH silver colloid. When using silver colloid, put the SH silver colloid in a centrifuge, set 10000r / min to centrifuge for ten minutes, absorb the supernatant liquid and discard it, and collect the silver nano sol in the middle and lower layers of the centrifuge tube for later use, which is the surface enhancement reagent;
[0031] 2) Put an appropriate amount of silver colloid into a centrifuge tube and centrifuge at high speed for 10 minutes, and remove the ...
Embodiment 2
[0037] Example 2 Surface Enhanced Raman Spectrum Detection of Saliva Throat Swab Specimens
[0038] (1) Preparation of surface enhancing reagent: same as step (1) of Example 1
[0039] (2) Processing of throat swab samples:
[0040] The saliva throat swab specimen is evenly dragged and coated on a flat, smooth, clean and pollution-free pure aluminum sheet, 2 μL of surface enhancement reagent is added dropwise, and allowed to dry naturally at room temperature to make a throat swab-surface enhanced Raman test piece . Store in a -80°C freezer or liquid nitrogen until Raman testing.
[0041] (3) Measurement of sample surface enhanced Raman spectrum:
[0042] Utilize the laser emitted by the micro-Raman spectrometer to irradiate the pharyngeal specimen on the throat swab-surface-enhanced Raman test piece dripped with the surface-enhanced reagent, and collect the Raman signal light emitted from the test piece at the same time, so as to detect the pharyngeal specimen at 400- 1800...
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