Method for detecting biochemical components of throat swab sample by adopting surface enhanced micro-Raman spectroscopy

A surface-enhanced Raman and surface-enhanced technology, which is applied in Raman scattering, material analysis through optical means, and measurement devices, can solve problems such as weak Raman spectrum signals, achieve good economic and social benefits, and improve feasibility Sex, process convenient and quick effect

Inactive Publication Date: 2016-07-06
FUJIAN NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the very weak Raman spectroscopy signal, the application of Raman spectroscopy in the field of biochemistry is subject to certain constraints.

Method used

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  • Method for detecting biochemical components of throat swab sample by adopting surface enhanced micro-Raman spectroscopy
  • Method for detecting biochemical components of throat swab sample by adopting surface enhanced micro-Raman spectroscopy
  • Method for detecting biochemical components of throat swab sample by adopting surface enhanced micro-Raman spectroscopy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Surface Enhanced Raman Spectrum Detection of Nasopharyngeal and Pharyngeal Swab Specimens

[0029] (1) Preparation of surface enhancing reagent:

[0030] 1) Take 5mL of hydroxylamine hydrochloride solution with a concentration of 0.06mol / L and add it to 4.5mL of sodium hydroxide solution with a concentration of 0.1mol / L; quickly add 90mL of nitric acid with a concentration of 0.0011mol / L to the prepared mixed solution In the silver solution, stir rapidly until the solution is uniform milky gray, which is SH silver colloid. When using silver colloid, put the SH silver colloid in a centrifuge, set 10000r / min to centrifuge for ten minutes, absorb the supernatant liquid and discard it, and collect the silver nano sol in the middle and lower layers of the centrifuge tube for later use, which is the surface enhancement reagent;

[0031] 2) Put an appropriate amount of silver colloid into a centrifuge tube and centrifuge at high speed for 10 minutes, and remove the ...

Embodiment 2

[0037] Example 2 Surface Enhanced Raman Spectrum Detection of Saliva Throat Swab Specimens

[0038] (1) Preparation of surface enhancing reagent: same as step (1) of Example 1

[0039] (2) Processing of throat swab samples:

[0040] The saliva throat swab specimen is evenly dragged and coated on a flat, smooth, clean and pollution-free pure aluminum sheet, 2 μL of surface enhancement reagent is added dropwise, and allowed to dry naturally at room temperature to make a throat swab-surface enhanced Raman test piece . Store in a -80°C freezer or liquid nitrogen until Raman testing.

[0041] (3) Measurement of sample surface enhanced Raman spectrum:

[0042] Utilize the laser emitted by the micro-Raman spectrometer to irradiate the pharyngeal specimen on the throat swab-surface-enhanced Raman test piece dripped with the surface-enhanced reagent, and collect the Raman signal light emitted from the test piece at the same time, so as to detect the pharyngeal specimen at 400- 1800...

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Abstract

The invention relates to a method for detecting component information of a throat swab sample by adopting surface enhanced micro-Raman spectroscopy. The method comprises preparation of a surface enhancement reagent, treatment of the throat swab sample, measurement of a surface enhanced Raman spectrum of the sample and processing and analysis of Raman spectrum data. According to the technical scheme, the method comprises the following steps: preparing the enhancement reagent for enhancing the Raman spectrum of the sample; smearing the throat swab sample on a smooth and clean pure aluminum sheet, dripping the surface enhancement reagent, and naturally air-drying the pure aluminum sheet at room temperature to prepare a throat swab-surface enhanced Raman test sheet; performing micro-Raman spectroscopic test on the throat swab-surface enhanced Raman test sheet to acquire the surface enhanced micro-Raman spectrum of the throat swab sample, and analyzing biochemical component information and features of the throat swab sample by virtue of spectral features. The method has the advantages of short detection time, low cost, convenience and rapidness in process and the like, and rich biochemical component information of the throat swab sample can be obtained, so that a rapid and effective analysis method can be provided for biochemical features and changes of intracavity mucous tissues.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for detecting biochemical components of throat swab specimens by using surface-enhanced micro-Raman spectroscopy. Background technique [0002] Pharyngeal swab specimen is to use a medical cotton swab to dip a small amount of secretions from the pharynx of the human body. The sample taken is a throat swab specimen. virus type. At present, the detection methods for throat swab specimens mainly include PCR method, rapid antigen method, etc. Although these methods have their own advantages, the operation method is complicated, time-consuming, and the detection sensitivity is not high. [0003] Raman spectroscopy is a non-destructive optical detection technology that can obtain rich fingerprint information such as molecular structure, vibration mode, and functional group, and is very sensitive to changes in the biochemical composition of proteins, nucleic acids, pho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 黄伟陈荣许智宏林多林学亮戈小松
Owner FUJIAN NORMAL UNIV
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