111results about How to "Analytical method is fast" patented technology

The invention discloses a method for studying metabolic difference of transgenic rice and non-transgenic rice. The method analyzes rice seed extract with liquid chromatography-mass spectrometry (LC-MS) technology to obtain metabolic profiling of rice, studies integral difference of metabolic profiling data between transgenic rice and non-transgenic rice by multivariate statistics method, and finds out compound with significant contribution to metabolic phenotype difference, to provide a basis for evaluation of unintended effect and safety of transgenic rice. The simple and rapid analysis method with good repeatability is suitable for batch analysis of practical samples.

The invention belongs to the technical field of food detection, and particularly relates to a fast instrumental analysis method for aflatoxins in foods. The invention discloses a fast instrumental analysis method for aflatoxins B1, B2, G1 and G2 in foods. The method comprises the following steps of: weighing a certain amount of sample; adding an acetonitrile-water solution in a certain ratio; performing ultrasonic extraction; and selecting specific parent ions and daughter ions by using a super-efficient liquid phase chromatography-mass spectrometer under a gradient elution condition to measure the content of the four types of aflatoxins. The method is simple and is high in efficiency and the precision of measurement results is high. By the method, four monomers of the aflatoxins are completely separated, and the recovery rate is high; and compared with the prior art, the method has the advantages of high speed and accuracy of detection and simple pretreatment, and can be used for accurately determining the nature and the quantity to meet requirements on research and detection.

The invention discloses a method for detecting content of short-chain or medium-chain chlorinated paraffin in a paint. The method comprises the following steps: extracting the short-chain or medium-chain chlorinated paraffin in the paint, detecting with a gas chromatography-negative chemical ionization source mass spectrometry detector, using the internal standard method, performing linear association on a total response factor of a short-chain or medium-chain chlorinated paraffin standard sample on the NCI (negative chemical ionization) mass spectrometry detector with the chlorine content of a short-chain or medium-chain chlorinated paraffin standard solution, detecting the actually measured chlorine content of the sample by the gas chromatography-negative chemical ionization source mass spectrometry detector, corresponding the actually measured chlorine content with the a liner reaction expression of the total response factor and the chlorine content of the short-chain or medium-chain, to obtain the total response factor of the sample, and thus obtaining the content of the short-chain or medium-chain chlorinated paraffin in the paint. The analysis method disclosed by the invention is exact and fast, has small interfering, and can perform optimal separation and qualitative and quantitative detections on each component in the sample.

The invention provides a near infrared spectrum qualitative identification method of pearl powder and shell powder. The near infrared spectrum qualitative identification method comprises the following steps of firstly, collecting near infrared spectrograms of a plurality of pearl powder samples and a plurality of shell powder samples as a pearl powder correction set and a shell powder correction set; then, establishing a discrimination analysis model by virtue of near infrared spectrum analysis software for setting model parameters according to the correction sets, wherein optical length types are selected for multi-element signal correction, spectrum pre-treatment is performed by virtue of a second derivative, smoothness is performed by virtue of Norris derivative filtering, a correction spectrum region is in a range of 4100-9800 cm<1> and the like; and finally, by virtue of the near infrared spectrograms of the samples to be detected and the established discrimination analysis model, analyzing the mahalanobis distance between the near infrared spectrograms of the samples to be detected and the pearl powder correction set, and the mahalanobis distance between the near infrared spectrograms of the samples to be detected and the shell powder correction set, and automatically giving an identification result by the near infrared spectrum software. The near infrared spectrum qualitative identification method provided by the invention is simple, convenient, fast and reliable.

The invention discloses a method for rapid detection of the residual mount of sulfanilamide in food. The method comprises the following steps: firstly enabling the sulfanilamide and fluorescamine to perform derivation reaction, further enriching derivative products by using cloud point extraction, adding a sample enrichment and a standard plate prepared from a sulfanilamide standard product on a thin layer silica gel plate, comparing the fluorescence intensities of the two under an ultraviolet lamp to judge the concentration range of the fluorescamine in a sample, and determining the residual amount of the sulfanilamide in the food, wherein a detection limit of the method is 0.08 micron g / mL. The method disclosed by the invention has the advantages of simplicity in operation, small using amount of organic solvent, high detection sensitivity, short detection time, strong specificity and capability of realizing effective separation from interfering substances, is a simple, convenient, fast and accurate analytical method, does not need large-scale instruments, only needs to configure small-scale instruments and equipment, and further has extensive application prospects.

The invention provides a method for testing the alkyl quaternary ammonium salt cationic, which includes the following steps: processing a pretreatment for the test sample; testing with a gas-chromatography-mass spectrometer, setting the injection port temperature of the instrument at 180-350 EDG C, setting the carrier gas flow rate at 0.5-2.0 ml per minute, the chromatographic column is a nonpolar, weak polar or strong polar column, setting the port temperature at 180-300 EDG C; injecting the pretreatmented sample into the chromatographic column through the injection port of the instrument, processing the programmed temperature, from 40-120 EDG C up to 210-320 EDG C, processing the mass spectrometric detection after each component of the decomposition products of the sample is separated by the chromatographic column. The method provided by the invention has the merits of cheap test instrument, safety, accurate and fast analysis method and small disturbance, which causes the alkyl quaternary ammonium salt cationic in the sample to pyrolyze on the injection port, and processes the optimized separation of each component of the pyrolyzed products.

The invention discloses a method for measuring dimethyl fumarate in a product by gas chromatography-mass spectrometry. The method comprises the following steps of: pretreating a dimethyl fumarate product to be detected by using an ultrasonic extraction method; setting the temperature of a sample injection port of a gas chromatography-mass spectrometer to be 240-280 DEG C, a flow rate to be 0.8-1.5 ml / min and the temperature of a port to be 280-300 DEG C, and selecting a weak polar column for a chromatographic column; enabling a sample to enter the chromatographic column through the sample injection port, and carrying out temperature programming on the temperature of the chromatographic column to separate the components of the sample by the chromatographic column; and enabling the components separated by the chromatographic column to enter a mass spectrometric detection part through the port of the gas chromatography-mass spectrometer, wherein an electron impact ion source is adopted as the ion source of the mass spectrometric detection. The method for measuring the dimethyl fumarate in the product by the gas chromatography-mass spectrometry has low detection limit, good detection effect, little consumption of analysis solvent and low detection cost.

The invention discloses a method for determination of phytic acid content of cotton seed powder. The method comprises collecting different kinds of cottonseed samples planted in different regions, carrying out sample husking, grinding, sieving and moisture balance on the samples, collecting full spectrum data, pretreating the near infrared spectroscopy data through a plurality of pretreatment methods, accurately determining sample phytic acid content through high performance ion chromatography, constructing the optimal PLS model in a full spectrum range through a full cross-validated method, carrying out variable selection on the spectroscopic data, building multiple correction models through a multivariate calibration regression method, building a near infrared spectroscopy correction model and detecting phytic acid content of cotton seed powder through the model. The method utilizes a Buchi NIR Flex-N500 Fourier transform near infrared spectrometer to acquire a spectrogram of cotton seed powder, has a fast determination speed and high accuracy, is environmentally friendly, convenient and efficient and has an important meaning for cultivation of low-phytic acid cotton and promotion of cotton side product processing and utilization.

The invention provides a method for detecting polycyclic aromatic hydrocarbon in food contact material by gas chromatogram-mass spectrometry coupling technique, comprising: Soxhlet extraction method or ultrasonic extraction method is used for extracting the detected food contact material; the extract sample is detected by a gas chromatogram-mass spectrometry coupling instrument; the temperature of a sample inlet of the instrument is set to be 240-320 DEG C, and the flow rate of carrier gas is set to be 0.5-2.0ml / min; a weakly polar chromatographic column is adopted, and the interface temperature is set to be 180-300 DEG C; the pretreated sample enters into the chromatographic column through the sample inlet of the instrument for temperature programming, and the temperature is raised to be 270-330 DEG C from 70-100 DEG C; after being separated by the chromatographic column, all the components in the sample are detected by mass spectrum. The method has the characteristics of low price of the detection instrument, safety, accurate and rapid analysis method and little interference as well as being suitable for standard analysis and detection which can lead all the components of the polycyclic aromatic hydrocarbon in the sample to be cracked in a mass spectrum ion source and optimize all the components of the cracked product, etc.

The invention discloses a food-borne pathogenic bacteria detection immuno-sensor and a preparation method thereof. The method comprises the following steps of: performing array spreading on DNA, attaching gold sol particles to the skeleton of the DNA, modifying specific antibodies on a DNA conductive chain, immobilizing the specific immune protein antibodies by using the strong adsorption property of metal granules, and fixing the ordered array structure of the DNA on the electrode layer. The invention provides the accurate and quick analysis method; and the sensor is cheap, portable and easy for popularization and use.

The invention discloses a biological immune sensor and a detection method thereof, which is a novel sensor technology achieving electrical signal detection of pathogen in foods mainly through an immune chemical reaction principle of specificity between an antibody and an antigen. The detection method of the biological immune sensor is accurate and fast, and a product of the biological immune sensor and the detection method of the biological immune sensor is cheap, portable and easy to popularize.

The invention discloses a chemical analysis method of rhodium content. The chemical analysis method is characterized by comprising the steps of: weighing a sample and arranging the sample in a crucible, adding anhydrous sodium carbonate, borax, silica, flour and lead oxide, adding sponge palladium powder and silver powder, putting the crucible into a furnace at 800 DEG C, heating to 1100 DEG C, carrying out heat preservation, removing the crucible to cool, taking out a lead button, putting the lead button into a cupel preheated in the furnace, and cupellating; and putting the generated particles into a container, adding HNO3 (nitric acid), heating and dissolving HNO3, adding HCl (hydrochloric acid), heating, evaporating, determining the volume and measuring rhodium content by ICP-AES (inductively coupled plasma-atomic emission spectrometry). The method is a simple and fast analysis method of rhodium content with an accurate result, and is suitable for measurement of 0.1-1000g / t of rhodium in various complicated samples, particularly suitable for fast measurement of rhodium content in materials such as anode mud containing silver, smelting slag and the like.

The invention discloses a wood star detection leveraging flight orbit optimization design method, which comprises the following steps of setting a flight sequence as earth-earth-wood star, and establishing an in-plane elliptical earth and wood star analysis ephemeris model; designing a detector and earth resonance emission algorithm, ensuring that the detector is emitted to leave the earth according to a given resonance ratio, establishing a transfer orbit model from deep space maneuver to earth leveraging by utilizing a Lanbert algorithm, setting an earth leveraging flying height, and establishing a transfer orbit model after earth leveraging; defining the earth borrowing moment and the emission moment as phase angle increments, and aiming at an emission window of a certain year, taking the emission moment, the resonance ratio and the phase angle increments as optimization variables to construct a multi-layer nested optimization strategy. Accurate guessing of the initial value of theoptimization variable can be realized, the robustness and rapidity of wood star detection transfer orbit optimization are effectively improved, and a rapid and effective analysis method is provided for rapid analysis and orbit optimization design of a launch window.

The invention relates to a method for automatic and comprehensive online analysis of genome sequences. The method comprises the following steps of 1, uploading sample data; 2, analyzing the sample data; 3, querying an analysis result of a single sample; 4, conducting combination analysis of multiple samples; 5, querying a combination analysis result. According to the method for the automatic and comprehensive online analysis of the genome sequences, an innovative mode of gene sequence data management is put forward, and not only can gene sequence data of multiple human individuals be stored simultaneously, but also it can be achieved that the gene sequence data corresponds to basic information of the individuals; a more accurate, faster and more comprehensive analysis method of the genome sequences is put forward, and the more complete annotation of genetic information is achieved; a novel, personalized and visualized online display mode of analysis results is put forward, and the method is suitable for ordinary people and doctors or research personals related to biomedicine and can well meet the needs of practical application.

The invention relates to a method for detecting component information of a throat swab sample by adopting surface enhanced micro-Raman spectroscopy. The method comprises preparation of a surface enhancement reagent, treatment of the throat swab sample, measurement of a surface enhanced Raman spectrum of the sample and processing and analysis of Raman spectrum data. According to the technical scheme, the method comprises the following steps: preparing the enhancement reagent for enhancing the Raman spectrum of the sample; smearing the throat swab sample on a smooth and clean pure aluminum sheet, dripping the surface enhancement reagent, and naturally air-drying the pure aluminum sheet at room temperature to prepare a throat swab-surface enhanced Raman test sheet; performing micro-Raman spectroscopic test on the throat swab-surface enhanced Raman test sheet to acquire the surface enhanced micro-Raman spectrum of the throat swab sample, and analyzing biochemical component information and features of the throat swab sample by virtue of spectral features. The method has the advantages of short detection time, low cost, convenience and rapidness in process and the like, and rich biochemical component information of the throat swab sample can be obtained, so that a rapid and effective analysis method can be provided for biochemical features and changes of intracavity mucous tissues.

The invention discloses a method for measuring bisphenol A in a product by gas chromatography-mass spectrometry. The method comprises the following steps of: soaking and extracting bisphenol A in a sample with an organic solvent, and then carrying out silanization and derivatization; detecting the bisphenol A in the sample with a gas chromatography-mass spectrometer: setting the temperature of a sample injection port to be 250-300 DEG C, a flow rate to be 0.8-1.5ml / min and the temperature of a port to be 280-300 DEG C; enabling the sample to enter the sample injection port, carrying out temperature programming on the temperature of the chromatographic column from 40-60 DEG C to 250-320 DEG C; and enabling the sample to enter a mass spectrometric detection part through the port to carry out mass spectrometric detection, wherein an electron impact ion source is adopted as the ion source of the mass spectrometric detection, the impact energy of the ion source is 70eV, and the temperature of the ion source is set to be 230-250 DEG C. The method for measuring the bisphenol A in the product has low detection limit, good detection effect, little consumption of analysis solvent and low detection cost.

The invention relates to a detection method for 2-methoxyethanol, 2-ethoxyethanol, 2-methoxyethanol acetate and 2-ethoxyethanol acetate, comprising the following steps: pretreating a sample with Soxhlet<,>s extraction method or ultrasound extraction method; detecting 2-methoxyethanol, 2-ethoxyethanol, 2-methoxyethanol acetate and 2-ethoxyethanol acetate with a gas chromatograph providing with a hydrogen flame ionization detector, with the injector temperature of 180-250 DEG C, high purity nitrogen as carrier gas, the carrier gas flow rate of 0.8-1.5ml / min, and polar column as chromatographic column; bringing the pretreated sample in the chromatographic column through the gas chromatograph for programming temperature from 40-80 DEG C to 220-240 DEG C; and separating the four objects from the chromatographic column. The method has the advantages of cheap detector, simple and safe operation, accurate and rapid analytical method.

The invention discloses an atmospheric environment chloride ion deposition rate testing device and method. The method comprises the steps of chloride ion deposition, chloride ion dissolution, chlorideion titration and blank sample determination, the chemical reagent silver nitrate used in the whole test process is low in toxicity, highly toxic and highly corrosive dangerous chemicals used in an existing method are avoided, and the test operation safety is high. The titration process is automatic, simple in flow and convenient to operate. The method can greatly save the test time, has high accuracy, reliability and safety, can quickly analyze and test a large amount of to-be-tested gauze in a short time, and improves the working efficiency of power grid atmospheric corrosion map construction of the State Grid Corporation of China.

The invention discloses a method for testing and analyzing characteristic parameters of a nanopore structure in a shale reservoir and a system thereof, The method includes the following steps: collecting a two-dimensional tomographic image of a shale sample; re-establishing a three-dimensional image based on the two-dimensional tomographic image of the shale sample; and obtaining the characteristic parameters of the shale nanopore structure based on the three-dimensional image. The technical scheme of the method utilizes a nano-CT technology to non-destructively detect the shale reservoir coreto obtain the shale pore structure image, and can accurately and quickly analyze the pore structure characteristic parameters of the shale reservoir core at the nanometer scale.

The invention claims a hollow-fiber-film-coated molecular imprinting integral adsorption rod, as well as a preparation method and an application thereof. According to the invention, an atrazine molecular imprinting capillary integral extraction head is prepared by using a way of in-situ microwave polymerization in a capillary; the imprinting integral extraction head is coated by using a hollow fiber film and then is used as a solid phase micro-extraction head; by combining a hollow fiver film extraction technology and a high performance liquid chromatography, parameters affecting the extracting efficiency are optimized, such as extraction and analysis solvents, salt concentrations and pH values, extraction and analyzing time, stirring speed and the like; two analysis methods capable of directly extracting triazine herbicides in an environmental water sample are established. The standard recovery rates of detected four triazine herbicide substances are greater than 80% under the optimized experiment conditions. According to the invention, a detection method in which hollow-fiber-film-coated molecular imprinting polymer integral column micro-extraction and an HPLC (High Performance Liquid Chromatography) are combined is established; the detection method has the advantages of simplicity, rapidity, high sensitivity and the like and is suitable for the routine analysis of the water samples.

The invention provides an analytic identification method for identifying that whether samples comprising a ginkgo leaf preparation, foods, healthcare products, medicines and / or cosmetics containing ginkgo leaf extracts and the like contain proanthocyanidins having other sources. The method is characterized in that a sample to be detected and a standard substance are pretreated through purifying with an polyamide resin, extracting with an organic solvent and the like; normal phase-high performance liquid chromatograms of the solution sample of the ginkgo leaf preparation to be detected and the solution substandard substance of a standard ginkgo leaf preparation are respectively established by adopting a phenomenex dihydric alcohol as a stationary phase, adopting a column having parameters of 250*4.6mm and 5mum, adopting (A)acetonitrile-(B)methanol / water / acetic acid (95 / 3 / 2, V / V / V) as a mobile phase, and adopting gradient eluting; and the chromatograms are contrasted and analyzed. The identification method has the advantages of high sensitivity, good resolution and reappearance, and obvious identification characteristic, and can accurately identify if the ginkgo leaf is added with above 0.5% of the proanthocyanidins having the other sources.

The invention discloses a method for measuring ethanolamine substance residual quantity in cosmetics. The method comprises the following steps of detecting a sample which is subjected to liquid-liquid extraction by using high performance liquid chromatography-tandem mass spectrometry instrument (HPLC-MS / MS), adding a formic ethanol solution in the sample, uniformly mixing in a volution manner, carrying out ultrasound extraction and centrifugal separation, taking a supernatant fluid and detecting by using the HPLC-MS / MS. The liquid chromatography conditions are that an amino liquid chromatography pillar is adopted, the column temperature is 30-60 DEG C, sample volume is 10-20mu L and the flowing speed of a flowing phase is 0.15-0.60mL / min; and the MS / MS conditions include an electric atomizing ion source, an cation mode ionization and a multi-ion reaction monitoring mode (MRM). The method provided by the invention has the characteristics that the extraction and purification are rapid, the selectivity of the method is high, the specificity of a chromatogram is strong, the interference is small, and the detection limit is low.

The invention relates to a method for measuring caprolactam, a harmful substance in the air of workplaces. The method for measuring the harmful substance caprolactam in the air of the workplace according to the present invention is to use a liquid chromatography-tandem mass spectrometer for detection. The method of using liquid chromatography-tandem mass spectrometry to detect the harmful substance caprolactam in the workplace air is convenient, fast, less disturbing, accurate and reliable, and has a low detection limit, which can meet people's detection work and research needs. The invention of this aspect fills up the blank of the liquid chromatography-tandem mass spectrometry (LC-MS / MS) method for measuring the harmful substance caprolactam in the air of the workplace.

The invention relates to a simple and convenient testing method for phenylamine in soil. The simple and convenient testing method for phenylamine in soil mainly comprises the steps of extracting, blowing, capturing and testing phenylamine in soil at two steps with acid and alkali. The method for extracting, blowing, capturing and testing trace phenylamine in soil at two steps with acid and alkali is simple, convenient, rapid, small in interference, accurate, reliable and low in detection limit, and the detection operation and study requirements of people can be met. By adopting the simple and convenient testing method, the blank of the method for extracting, blowing, capturing and testing trace phenylamine in soil at two steps with acid and alkali is made up.

The invention discloses a method for determining the content of nickel in nickel ferrite. The method comprises the following steps of: (1) accurately weighing a nickel ferrite sample, decomposing the nickel ferrite sample by using dilute nitrohydrochloric acid, and dissolving the treated sample by fuming perchloric acid to obtain a sample solution; (2) treating a standard sample by using a method adopted in the step (1), determining the spectral line intensity of an obtained standard sample solution, and drawing a standard curve of the spectral line intensity-nickel content; and (3) determining the spectral line intensity of the nickel in a sample test solution so as to obtain the content of the nickel in the sample according to the standard curve. The sample is dissolved by using the dilute nitrohydrochloric acid and the perchloric acid, and the matrix interference is reduced by fuming the perchloric acid, so that the analysis can be performed by using the inductively coupled plasma-atomic emission spectrometry (ICP-AES), the defects of complex operation and long analysis cycle of a chemical wet method are overcome, and the method has the advantages of high sensitivity, low interference, quickness in analysis, and accurate and reliable analysis result, and is applicable to the daily analysis detection of an enterprise.

The invention provides a spur gear tooth root crack propagation rule analysis method based on finite element simulation. The method comprises the following steps of firstly, establishing a gear simulation model by utilizing ANSYS, then completing load loading, mesh generation, boundary condition setting, stress solving and other work on the basis of the model, importing the model into FRANC3D to obtain data such as an expansion path and direction of a gear tooth root crack, stress intensity factor change in a crack expansion process, crack life prediction and the like, and finally, analyzing afinite element simulation result to obtain a spur gear tooth root crack propagation rule. According to the method, the propagation rule of the spur gear tooth root cracks is researched based on a numerical simulation method, the crack propagation situation is tracked, the distribution rule of crack tip stress intensity factors and a fatigue life prediction curve are obtained, the calculation precision is high, the experiment speed is higher, the efficiency is higher, and convenience is brought to the depth research of gear tooth root crack propagation.

The invention relates to a quality control method of a traditional Chinese medicine preparation. Particularly, an HPLC (high performance liquid chromatography) is used for separation, an MS (mass spectrum) is used for detection, MRM (multiple reaction monitoring) is combined, an LC-MS-MRM separation, detection and statistical identification combined quality control model for a Shuxuetong injection is established, and the model is used for performing quality control on polypeptide (oligopeptide) components in the Shuxuetong injection. Qualitative identification can be performed on the known active polypeptide (oligopeptide) components in the Shuxuetong injection quickly and effectively with the method, and the method is simple, quick, high in specificity, good in reproducibility and more beneficial to improvement on a quality control means for the Shuxuetong injection.

The analysis process of silver in copper anode slime includes adding HNO3 into sample and heating to decompose, adding HCl, heating to evaporate, cooling, adding water, heating to boil, cooling, filtering with quantitative filter paper, washing container and deposit, setting the deposit together with the filter paper into the container, adding H2SO4, carbonizing, adding HNO3 until the solution become colorless, heating to smoke with dense white smoke, cooling, diluting with water, adding ferric ammonium sulfate as indicator, and titrating with standard potassium thiocyanate solution to light red as the terminal. The method is simple, fast, accurate, and suitable for the analysis of silver in copper anode slime.

The invention relates to an infrared spectral feature based chemical rapid discrimination method and device. The device consists of an optical path module composed of a light source, a spectrometer and optical fiber, a mainboard unit, a touchable display screen, and a software analysis unit, wherein the analytical algorithm part applies a chemometrics method, and the model establishment and replacement can be very convenient. The method and the device are mainly used for automatic collection and rapid discrimination of chemical spectral information, and the device has the characteristics of portability, strong stability, and humanized interactive interface, etc.

The invention provides a method for increasing yield of 5-sulfoisophthalic acid for synthesis of a ternary monomer. The method comprises the following steps: feeding, filling nitrogen, performing a first-stage heating reaction, performing a second-stage heating reaction, adding remaining oleum, performing a third-stage heating reaction, and performing a negative pressure reaction. The method has the beneficial effects that the reaction time is shortened, and the reaction temperature is lowered; compared with the existing production process, the maximum reaction temperature is lowered by 30 DEGC, and the sulfonation reaction cycle is shortened by 2 hours; the loss of sulfur trioxide is reduced, the cost is reduced, and the economic benefits of the ternary monomer product can be increased;the amount of oleum is reduced, which is 5% less than that in the current process dosage, and the amount of alkali used in the subsequent neutralization process can be reduced by more than 5%, at thesame time, the reaction time of the neutralization process is shortened, the amount of cooling water is reduced, and the energy saving effect is remarkable; and the yield of 5-sulfoisophthalic acid isincreased.