Method for studying metabolic difference of transgenic rice and non-transgenic rice

A transgenic rice, non-transgenic technology, applied in the field of analytical chemistry, can solve problems affecting the credibility of final results, experimental data deviation from the real value, mass spectrometry response signal deviation, etc., to save identification time, fast scanning speed, and avoid human errors Effect

Inactive Publication Date: 2012-05-30
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
View PDF2 Cites 19 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the pretreatment process is carried out in multiple steps, it is easy to introduce errors in the sample preparation process; if the number of samples is relatively large, the mass spectrometry will run for a long time, and due to the attenuation effect of the electrospray ion source itself, the mass spectrometry response signal of the sample will have a certain deviation
This will cause the experimental data to deviate from the true value and affect the credibility of the final result

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for studying metabolic difference of transgenic rice and non-transgenic rice
  • Method for studying metabolic difference of transgenic rice and non-transgenic rice
  • Method for studying metabolic difference of transgenic rice and non-transgenic rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Rice sample collection

[0024] The transgenic rice sample is a Marker-free bivalent insect-resistant strain material; including 3 strains, namely N6080, N6049, and N6130, which are excellent strains transformed by Minghui 86 as a recipient, after continuous multi-generation selfing and field selection Tie. Both the genetically modified rice and the non-transgenic rice Minghui 86 are planted in Hainan, sowed and harvested at the same time. Rice samples were collected and stored in a -80°C refrigerator.

[0025] 2. Analysis method

[0026] 2.1 Pretreatment of rice seed samples

[0027] The rice seed samples were taken out from the -80 °C refrigerator and waited for their temperature to return to room temperature in a desiccator. Each rice seed is dehulled, ground in a mortar, and passed through a 60-mesh sieve to obtain a powder with uniform particle size. Divide the rice powder into plastic tubes and set aside.

[0028] Weigh 5 parts of N6080 or N6049 (0.1500 g...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for studying metabolic difference of transgenic rice and non-transgenic rice. The method analyzes rice seed extract with liquid chromatography-mass spectrometry (LC-MS) technology to obtain metabolic profiling of rice, studies integral difference of metabolic profiling data between transgenic rice and non-transgenic rice by multivariate statistics method, and finds out compound with significant contribution to metabolic phenotype difference, to provide a basis for evaluation of unintended effect and safety of transgenic rice. The simple and rapid analysis method with good repeatability is suitable for batch analysis of practical samples.

Description

technical field [0001] The invention relates to the field of analytical chemistry, and is a method for studying metabolic differences between transgenic and non-transgenic rice based on rice metabolic profile of metabolomics technology. Background technique [0002] The rapid development of transgenic technology has sparked debates on the impact of transgenic plants on human health and the ecological environment. At present, people's concerns about the safety of genetically modified plants are mainly reflected in the following aspects: (1) How toxic the foreign gene is; (2) Whether it is allergic; (3) Whether it produces toxic substances; (4) Whether it affects the nutritional value. Influence. In order to ensure the safety of genetically modified plants, many countries including the United States, the European Union, Japan and my country, as well as some international organizations such as FAO and WHO have formulated a series of management measures for the safety of geneti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 许国旺常玉玮赵春霞路鑫周佳赵燕妮
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap