Method for studying metabolic difference of transgenic rice and non-transgenic rice

A transgenic rice, non-transgenic technology, applied in the field of analytical chemistry, can solve problems affecting the credibility of final results, experimental data deviation from the real value, mass spectrometry response signal deviation, etc., to save identification time, fast scanning speed, and avoid human errors Effect
CN102478563AInactive Publication Date: 2012-05-30DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Publication Date
2012-05-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for studying metabolic difference of transgenic rice and non-transgenic rice. The method analyzes rice seed extract with liquid chromatography-mass spectrometry (LC-MS) technology to obtain metabolic profiling of rice, studies integral difference of metabolic profiling data between transgenic rice and non-transgenic rice by multivariate statistics method, and finds out compound with significant contribution to metabolic phenotype difference, to provide a basis for evaluation of unintended effect and safety of transgenic rice. The simple and rapid analysis method with good repeatability is suitable for batch analysis of practical samples.
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Description

technical field

[0001] The invention relates to the field of analytical chemistry, and is a method for studying metabolic differences between transgenic and non-transgenic rice based on rice metabolic profile of metabolomics technology. Background technique

[0002] The rapid development of transgenic technology has sparked debates on the impact of transgenic plants on human health and the ecological environment. At present, people's concerns about the safety of genetically modified plants are mainly reflected in the following aspects: (1) How toxic the foreign gene is; (2) Whether it is allergic; (3) Whether it produces toxic substances; (4) Whether it affects the nutritional value. Influence. In order to ensure the safety of genetically modified plants, many countries including the United States, the European Union, Japan and my country, as well as some international organizations such as FAO and WHO have formulated a series of management measures for the safety of geneti...

Claims

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