281 results about "Transgenic technology" patented technology

The invention belongs to the technical field of biology, and particularly relates to application of a soybean GmSRO5 gene in promoting generation of leguminous plant root nodules. A pZP211 overexpression vector is utilized, a transgenic plant is rapidly obtained through agrobacterium tumefaciens-mediated soybean genetic transformation, GmSRO5 is successfully overexpressed, root nodule number statistics is carried out on a chimera transgenic plant, and a result shows that the GmSRO5 gene can positively regulate and control the number of soybean root nodules and has a remarkable effect. The invention proposes that SRO5 can positively regulate and control the soybean nodulation process for the first time, and proves that the soybean GmSRO5 gene overexpression can significantly increase the number of soybean root nodules, and the soybean GmSRO5 gene overexpression can be applied to soybean production and molecular breeding through transgenosis, molecular marking and other means in the future, and has very important application value.

The invention discloses a gene CsIAA4 for improving parthenocarpy capacity of cucumbers and application of the gene CsIAA4, and identifies a novel parthenocarpy regulating gene CsIAA4 of the cucumbers, and the nucleotide sequence of the gene CsIAA4 is as shown in SEQ ID NO.1. The parthenocarpy ability of the gene editing mutant is weakened, and the parthenocarpy ability of the overexpression mutant is remarkably enhanced. The gene CsIAA4 can be used for guiding breeding and improvement of cucumber strong parthenocarpy germplasm resources, and technical support is provided for breeding of cucumber parthenocarpy varieties. The application of the CsIAA4 in cucumber parthenocarpy is jointly explored through two transgenic technologies, a new gene resource is provided for genetic improvement of cucumber parthenocarpy molecules, the cucumber parthenocarpy gene CsIAA4 is provided, and the cucumber parthenocarpy gene CsIAA4 has important application value in the aspect of research and improvement of cucumber parthenocarpy capacity.

The invention belongs to the technical field of genetic engineering in molecular biology, and relates to a gene for regulating and controlling the sugar content of strawberry fruits and application of the gene. According to the invention, the FveSMXL7 gene is cloned from forest strawberries and is used for constructing a silent vector of the FveSMXL7 gene, and a stable transgenic technology proves that the sugar content of strawberry fruits can be increased by reducing the expression of the FveSMXL7 gene, which indicates that the gene has the function of regulating and controlling the sugar content of the strawberry fruits.

The invention belongs to the technical field of gene engineering, and particularly discloses a waterlogging-resistant gene Di19 and application thereof in waterlogging-resistant breeding of oilseed rape, the Di19 gene of Essel Russian mustard is transferred into oilseed rape for the first time by utilizing a transgenic technology, and the waterlogging resistance of the obtained transgenic oilseed rape is remarkably improved compared with that of wild type oilseed rape. Therefore, the invention provides a new gene resource for breeding the waterlogging-resistant rape.

The invention relates to the technical field of plant genetic engineering, and discloses application of a soybean low-phosphorus-tolerant gene GmPP2-10 in promoting growth of plants in a low-phosphorus stress environment. Researches show that the expression of the GmPP2-10 gene is increased under the induction of low phosphorus stress, and under different phosphorus concentration treatment conditions, the over-expression of the GmPP2-10 can increase the biomass of a transgenic plant and promote the growth of the plant under the low phosphorus condition; meanwhile, overexpression of GmPP2-10 can improve the tolerance of plants to low-phosphorus stress, and reduce the inhibition effect of low phosphorus on plant root growth. Therefore, the GmPP2-10 plays an important role in adapting to the low-phosphorus stress of the plant, and the adaptive capacity of the plant to the low-phosphorus stress of the acid soil can be improved by transferring the GmPP2-10 into the plant through a transgenic technology.

The invention discloses an effect protein E56, a coding gene and application of the effect protein E56 to improvement of southern rust resistance of corn, and belongs to the technical field of agricultural biology. The amino acid sequence of the effector protein E56 is as shown in SEQ ID NO. 1. A reverse genetics method is utilized to analyze the Puccinia polystachys effect protein E56 gene, and the E56 gene is up-regulated in expression in the Puccinia polystachys infection process. The E56 gene is silenced by adopting a host-mediated gene silencing technology, and the toxic function of the E56 gene in the infection process of the southern rust disease is determined. A specific fragment of the E56 gene is cloned to an RNAi interference vector, a corn immature embryo is transformed by using an agrobacterium tumefaciens-mediated transgenic technology, and an obtained transgenic corn plant shows resistance to the corn southern rust disease. The E56-RNAi transgenic plant shows resistance to puccinia polypoda, so that the effect protein E56 can be used for creating a new strain for resisting the southern rust disease.

The invention discloses an application of a multidrug-resistant efflux transporter gene OsMRET1 in regulation and control of chalkiness traits of rice. A nucleotide sequence of a coding region of the gene is shown as SEQ ID No.1, and a coded amino acid sequence is shown as SEQ ID No.2. The invention further discloses an application of the multidrug-resistant efflux transporter gene OsMRET1 in regulation and control of chalkiness traits of rice. It is found for the first time that the gene OsMRET1 can regulate and control formation of chalkiness, an OsMRET1 gene knockout mutant is created through a gene editing technology, an OsMRET1 gene overexpression strain is obtained through a transgenic technology, it is verified that the OsMRET1 gene is a new gene for regulating and controlling rice chalkiness, and overexpression of the gene can reduce rice chalkiness and improve rice chalkiness. And a new gene resource and a technical route are provided for rice quality improvement.

The invention discloses a plant immunomodulatory gene RDP as well as an expression vector and application thereof. The inventor finds a plant immunomodulatory factor gene RDP from potatoes, after a corresponding sequence is obtained through cloning, agrobacterium competent cells are transferred into nicotiana benthamiana, it is verified that the plant immunomodulatory factor gene RDP can be successfully expressed in other plants, and it is verified that the pathogenic effects of various phytophthora are good. The gene is proved to be capable of effectively improving the disease resistance of the nicotiana benthamiana after being expressed. Through objective evaluation of an agrobacterium tumefaciens-mediated transient expression system, it is found that the plant immunomodulatory factor RDP can induce plants to generate resistance, the gene can be directly introduced into the plants through a transgenic technology due to the powerful function of the gene, disease-resistant varieties are developed and cultivated, and the gene has great significance in prevention and treatment of plant diseases.

The invention relates to the technical field of plant tissue culture and plant transgenosis, in particular to a method for co-transformation of an LcSUCLA alpha-LcSUCLA beta-pEGFC binary expression vector and Leymus chinensis double genes. The method comprises the following steps: obtaining leymus chinensis tissue culture sterile calluses, transforming agrobacterium competent by a freeze thawing method, activating agrobacterium tumefaciens, infecting the leymus chinensis calluses and co-culturing, screening and culturing plant transformation materials, differentiating and screening a culture medium and culture conditions, and differentiating and screening a culture medium and culture conditions as well as a rooting culture medium and culture conditions. According to the invention, double-gene co-transformation of leymus chinensis is realized, and a leymus chinensis double-transgenic plant is obtained. The cotransformation of the LcSUCLA alpha and the LcSUCLA beta in the leymus chinensis can synthesize more succinic acid than the single transformation, so that the tolerance of the leymus chinensis under the stress of basic salt is further improved.

The invention discloses an application of an OsPAHX5 gene and a protein coded by the OsPAHX5 gene in regulation and control of rice grain length, and belongs to the technical field of plant breeding and biology. The invention discloses a nucleotide sequence of a coding region of an OsPAHX5 gene, a coded amino acid sequence, a nucleotide sequence of a CRISPR-Cas9 knockout target site, a nucleotide sequence of a knockout mutant and an amino acid sequence of the knockout mutant, which are shown in an ST.26 standard sequence nucleotide or amino acid sequence table. The function of the OsPAHX5 gene is researched by utilizing molecular biology and a plant transgenic technology, and it is found that after the OsPAHX5 gene is knocked out by utilizing a CRISPR-Cas9 technology, the grain length of a knocked-out transgenic plant is remarkably larger than that of a wild type variety; after the OsPAHX5 gene is subjected to overexpression by using a transgenic overexpression technology, the grain length of an overexpressed transgenic plant is not obviously different from that of a wild type variety. According to the invention, the OsPAHX5 gene can be utilized to create a rice variety with larger grain length.

The invention discloses rice LOC_Os01g68930 The application of genes in regulating the protein content in rice grains relates to the field of genetic engineering technology. LOC_Os01g68930 The CDS sequence of the gene is shown in SEQ ID No. 1, and the amino acid sequence of the protein encoded by it is shown in SEQ ID NO. 2. The present invention obtains OE‑LOC_Os01g68930 Overexpression plants. Experiments have shown that: PLACE_ Os01g68930 The total content of glutenin and alcohol-soluble proteins in the seeds of overexpressing transgenic rice reached 7.88% to 7.97%. In comparison, the protein content of wild-type rice grains was 6.97%, and the protein content of transgenic rice grains increased by 13.1%-14.3%. LOC_Os01g68930 It has been confirmed that this gene can effectively regulate the protein content of rice grains, laying a solid foundation for the subsequent breeding of high-protein rice varieties.

The invention relates to the technical field of plant genetic engineering, and discloses a plant low-phosphorus-resistant important gene GsMYB7 and application thereof. According to the invention, an MYB family GsMYB7 gene is cloned in soybean for the first time. Researches show that the expression of the GsMYB7 gene is increased under the induction of low phosphorus stress, and under different phosphorus concentration treatment conditions, the overexpression GsMYB7 can obviously increase the biomass of transgenic plants and promote the growth of the plants under the low phosphorus condition; meanwhile, overexpression of GsMYB7 can improve the tolerance of plants to low-phosphorus stress, and reduce the inhibition effect of low phosphorus on plant root growth. Therefore, the GsMYB7 plays an important role in adapting to the low-phosphorus stress of the plant, and the adaptive capacity of the plant to the low-phosphorus stress of the acid soil can be improved through a transgenic technology.

The invention discloses genetic engineering application of a rice ethylene response transcription factor OsKTR2. The invention discloses a rice ethylene response transcription factor OsKTR2, and the CDS sequence of the rice ethylene response transcription factor OsKTR2 is SEQ ID NO.1. In the invention, it is found for the first time that a recombinant expression vector constructed by the OsKTR2 gene is transfected into wild rice (Oryza sativa. Ssp.cv.Japonica) through an agrobacterium-mediated rice transgenic technology, and the effective tiller number, the total tiller number and the maturing rate of rice are increased, so that the yield of rice is increased. In addition, the expression of the OsKTR2 is induced to be up-regulated by low potassium stress, so that the potassium absorption of the rice is promoted.

The invention discloses application of a TaSPL18-5D protein and a related biological material thereof to regulation and control of the ear length and the yield of a plant. According to the invention, a TaSPL18-5D overexpression wheat strain is obtained through a transgenic technology, and then analysis on the TaSPL18-5D overexpression wheat strain finds that compared with wild type wheat KN199, the TaSPL18-5D overexpression wheat strain has the advantages that the ear length is shortened, and the spikelet number and the ear grain number are obviously reduced. Therefore, the TaSPL18-5D protein can negatively regulate the ear length, the spikelet number and the grain number per ear of the plant, a new target is provided for improving the plant type and the yield of the wheat after green revolution, and the TaSPL18-5D protein has important significance for cultivating high-yield wheat varieties in agricultural production.

The invention provides an application of a rape RPM1 interaction protein BnaRIN4 in regulating and controlling the oil content of rape seeds. On the basis of whole genome association analysis and whole transcriptome association analysis, a candidate gene BnaRIN4 for regulating and controlling the oil content is predicted, a homozygous mutant material with deletion of the gene BnaRIN4 function is further created through a transgenic technology, and the gene plays an important role in negative regulation and control of the oil content of the rape seeds, so that the application of the gene in negative regulation and control of the oil content of the rape seeds is realized. By specifically knocking out or mutating the gene, a novel rape germplasm of which the oil content of the seeds is remarkably improved can be obtained. The invention discloses the biological function of the gene in regulating and controlling the oil content of rape seeds for the first time, and provides theoretical basis and technical support for molecular breeding of high-oil-content rape varieties.

The invention discloses a method for constructing a miR-181a-2 knockout mouse animal model based on CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 and application of the method, and belongs to the technical field of transgenosis. According to the invention, two sgRNAs of a specific targeting non-coding gene miR-181a-2 are designed, the sgRNAs comprise an sgRNA 1 with a nucleotide sequence as shown in SEQ ID NO.2 and an sgRNA 4 with a nucleotide sequence as shown in SEQ ID NO.5, bidirectional knockout is carried out by combining Cas9 protein, and a miR-181a-2 knockout mouse strain capable of stably breeding is obtained. Experimental results show that the knockout method provided by the invention does not generate off-target in preparation of the mouse model, and has the advantages of accuracy, high efficiency, simplicity and convenience. An oral cancer model is constructed by using the knockout strain, so that the modeling time is shortened, the research cost is reduced, and the knockout strain has remarkable advantages in the aspects of scientific research efficiency and achievement transformation and provides great convenience for related treatment of oral cancer.

The invention discloses application of a cotton GbbHLH35 gene in improving the disease resistance of plant verticillium wilt, the nucleotide sequence of the gene is shown as SEQ ID NO: 1, and the amino acid sequence is shown as SEQ ID NO: 2. The gene is remarkably and highly expressed in cotton roots and is remarkably up-regulated under the induction of verticillium wilt, and the sequence of the gene contains a conserved motif related to disease resistance. The sensitivity of the plant to verticillium wilt is enhanced by silencing the expression of the GbbHLH35 gene in the sea island cotton. The overexpressed GbbHLH35 gene arabidopsis thaliana homozygous line is obtained through a transgenic technology, and after the overexpressed GbbHLH35 gene arabidopsis thaliana homozygous line is infected with verticillium The invention has important significance in improvement of cotton verticillium wilt resistance breeding and research, and can be applied to breeding of resistant cotton varieties.

The invention belongs to the technical field of plant breeding in molecular biology, and provides a cotton GhVIP1 gene and application thereof in drought resistance and salt tolerance of plants. The nucleotide sequence of the GhVIP1 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the GhVIP1 gene is as shown in SEQ ID NO: 2. The gene is obviously up-regulated under the induction of salt and drought stress. An arabidopsis thaliana homozygous line overexpressed with the GhVIP1 gene is obtained through a transgenic technology, under the stress of drought and salt, the seed germination rate of the arabidopsis thaliana overexpressed with the gene is higher than that of a wild type, the growth condition of a transgenic line in the seedling stage is better than that of the wild type, the stem length, the total antioxidant capacity and the chlorophyll content are all superior to those of the wild type, and the MDA content is lower than that of the wild type; further, the drought resistance and the salt resistance of the arabidopsis thaliana are improved. When the gene is applied to drought-resistant and salt-resistant breeding of plants, the drought resistance and salt resistance of the plants can be improved, and an effective alternative scheme is provided for molecular breeding of drought-resistant and salt-resistant crops.

The invention relates to a DNA (Deoxyribose Nucleic Acid) for regulating and controlling a resistance gene LTPL160 of rice bacterial blight and breeding utilization of a protein of the DNA. According to the invention, the gene OsLTPL160 for regulating and controlling the resistance of the rice bacterial leaf blight is cloned by virtue of a non-gel quantitative proteomics analysis and transcription analysis method. The OsLTPL160 knockout and overexpression material is obtained by adopting a gene editing and transgenic technology. The OsLTPL160 is knocked out, so that the bacterial blight resistance of the rice is remarkably improved; and overexpression of the OsLTPL160 obviously reduces the resistance of the bacterial blight of the rice. The cloning of the OsLTPL160 gene and the construction of the material provide material and technical support for the bacterial blight resistance of the rice.

The application belongs to the technical field of genetic engineering, and particularly discloses AtDi19 Application of the gene in drought resistance and high-oil rape breeding AtDi19 The gene is first transferred into the rape by using the transgenic technology, and the transgenic rape obtained has significantly improved drought resistance compared with the wild type rape; and the oil content of the rape seed is significantly increased under the drought stress. Therefore, the application provides a new gene resource for drought-resistant and stable high-oil rape breeding.

The invention discloses application of a CsDDM1 gene in regulating and controlling the length of cucumber fruits, and relates to the technical field of bioengineering breeding. The nucleotide sequence of the CsDDM1 gene is as shown in SEQ ID NO: 1. The CsDDM1 gene participating in cucumber fruit length regulation and control is identified and verified, then a cucumber CsDDM1 gene knockout material is constructed by utilizing a transgenic technology, and function research shows that the cucumber fruit becomes shorter by knocking out the CsDDM1 gene, so that the mechanism blank of epigenetic regulation and control of the character is filled, the commodity and ornamental value of the cucumber are increased, and the cucumber CsDDM1 gene knockout material has good application prospects. The method solves the problems that traditional breeding is long in period and short fruit germplasm is deficient, breeding efficiency is improved, germplasm resources are enriched, the research result also provides a new thought for character improvement of other crops, and scientific and technological progress and innovative development of the whole field are promoted.

The invention discloses a gene for promoting low-nitrogen efficient utilization of rice and enlarging grains and application of the gene. The gene is derived from rice and is named as OsUGT, and the nucleotide sequence of the gene is shown as SEQ ID NO.1; the amino acid sequence of the protein coded by the gene is as shown in SEQ ID NO. 2. The invention provides a construction method of an OsUGT gene overexpressed transgenic rice strain, which comprises the following steps: constructing pRHVc-OsUGT recombinant plasmids, transforming the pRHVc-OsUGT recombinant plasmids into calluses through an agrobacterium tumefaciens-mediated method, and screening resistant calluses and regenerating plants to obtain the OsUGT gene overexpressed rice. The OsUGT gene is overexpressed in a rice plant by adopting a pRHVc vector through a transgenic technology, so that the nitrogen utilization efficiency of the plant in a medium-low nitrogen stress environment can be remarkably improved, grain enlargement and grain weight increase can be effectively promoted, stable improvement of the crop yield under a low-nitrogen condition is realized, and the technical effect is clear and efficient.

The invention discloses a gene OsNST2 for transporting nucleotide sugar and application of the gene OsNST2. A nucleotide sequence of a coding region of the gene is as shown in SEQ ID No. 1, and an amino acid sequence coded by the gene is as shown in SEQ ID No. 2. It is found for the first time that the growth, development and yield of rice are affected by the OsNST2 by regulating and controlling formation of rice cell walls, an OsNST2 gene knock-down strain is created through an RNAi technology, an OsNST2 gene overexpression strain is obtained through an overexpression transgenic technology, it is verified that the OsNST2 gene is a new gene for regulating and controlling formation of the rice cell walls, and a new gene resource is provided for high yield of rice.

The invention discloses a transcription factor TgWRKY65 of torreya grandis as well as a coding gene and application of the transcription factor TgWRKY65. The amino acid sequence of the transcription factor TgWRKY65 is shown as SEQ ID NO.2, and the nucleotide sequence of the coding gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the transcription factor TgWRKY65. According to the invention, a homologous expression material is constructed through cloning of the TgWRKY65 gene, a transgenic technology and gene silencing, and the application prospect in the aspect of increasing the content of the torreya grandis catechin is great; when the coding gene is over-expressed, the content of catechin is increased; when the coding gene is silenced, the content of catechin is reduced.

The invention belongs to the technical field of plant transgenosis, and particularly relates to a soybean genetic transformation method which comprises the following steps: removing terminal buds and all lateral buds of soybean seedlings, dripping agrobacterium containing target genes on growing points without the lateral buds to realize soybean genetic transformation. T0-generation transgenic seedlings can be obtained within 180 days or more, and the T0-generation transgenic seedlings can be obtained within 40 days, so that the genetic transformation period is greatly shortened; in terms of transformation efficiency, soybean genetic transformation is difficult, the traditional genetic transformation efficiency is about 5%-10%, and the genetic transformation efficiency of the method is stabilized to be 20% or above.

The invention discloses application of a cotton Gh4CL7 gene in preparation of a cotton variety with improved or reduced high-temperature fertility, and belongs to the technical field of plant genetic engineering. The Gh4CL7 gene has a nucleotide sequence as shown in SEQ ID NO: 1 and can encode an amino acid sequence as shown in SEQ ID NO: 2. According to the invention, a Gh4CL7 gene transgenic cotton plant is obtained through a transgenic technology, so that the fertility of the cotton plant at high temperature is influenced. Furthermore, through paraffin section cytological observation and field test yield trait analysis, the result shows that the Gh4CL7 gene plays an important role in regulating and controlling high-temperature fertility. The invention provides favorable gene resources for cultivation of high-temperature-resistant cotton varieties.

The invention provides a highland barley HvDREB3 protein, a coding gene and application thereof, and belongs to the technical field of plant genetic engineering. The HvDREB3 protein provided by the invention has an amino acid sequence as shown in SEQ ID NO.1, and the coding gene of the HvDREB3 protein has a nucleotide sequence as shown in SEQ ID NO.2. The invention also provides application of the protein, the coding gene of the protein or a biological material containing the coding gene in regulation and control of plant freezing resistance, and a method for constructing an anti-freezing plant. The HvDREB3 gene is subjected to overexpression in highland barley through an overexpression transgenic technology, so that the survival rate is remarkably increased, the ion leakage rate is remarkably reduced, the chlorophyll content is increased, the antioxidant enzyme activity is enhanced, and the capability of resisting low-temperature freezing injury stress is remarkably improved.

The invention discloses an application of a related gene Zm00001eb129860 in the flowering period of corn in regulation and control of flowering transformation. According to the invention, a high-expression gene Zm00001eb129860 related to the flowering period of corn is screened from transcriptome data of inflorescence meristem in a key period of flowering transformation of corn, the gene affects the change process of the flowering transformation of corn, and a gene knockout mutant plant has a late flowering phenotype; when the gene is over-expressed in a corn plant, a transgenic plant with early flowering transformation can be obtained, so that the flowering period of the corn is shortened, genetic improvement and breeding of the plant are facilitated, and the gene has important significance for promoting early flowering of the corn. It is indicated that Zm00001eb129860 plays an important role in advancing corn flowering transformation, can be used for regulating and controlling the flowering period of plants through a transgenic technology, facilitates cultivation of early flowering plants and facilitates genetic improvement of the plants.

The invention relates to the technical field of agricultural transgenosis, and particularly discloses application of a GhDBB28 gene in regulating and controlling salt tolerance of upland cotton plants, and the nucleotide sequence of the GhDBB28 gene is as shown in SEQ ID NO.1; the sensitivity of upland cotton plants to salt is improved by silencing the GhDBB28 gene. The activity of an anti-oxidation system in the upland cotton plant is reduced through silencing of GhDBB28, so that the plant is more sensitive to salt stress, and a scientific basis is provided for constructing a new plant type of a salt-sensitive transgenic upland cotton plant.