168 results about "Transgenic technology" patented technology

The invention discloses an effect protein E56, a coding gene and application of the effect protein E56 to improvement of southern rust resistance of corn, and belongs to the technical field of agricultural biology. The amino acid sequence of the effector protein E56 is as shown in SEQ ID NO. 1. A reverse genetics method is utilized to analyze the Puccinia polystachys effect protein E56 gene, and the E56 gene is up-regulated in expression in the Puccinia polystachys infection process. The E56 gene is silenced by adopting a host-mediated gene silencing technology, and the toxic function of the E56 gene in the infection process of the southern rust disease is determined. A specific fragment of the E56 gene is cloned to an RNAi interference vector, a corn immature embryo is transformed by using an agrobacterium tumefaciens-mediated transgenic technology, and an obtained transgenic corn plant shows resistance to the corn southern rust disease. The E56-RNAi transgenic plant shows resistance to puccinia polypoda, so that the effect protein E56 can be used for creating a new strain for resisting the southern rust disease.

The invention discloses an application of a multidrug-resistant efflux transporter gene OsMRET1 in regulation and control of chalkiness traits of rice. A nucleotide sequence of a coding region of the gene is shown as SEQ ID No.1, and a coded amino acid sequence is shown as SEQ ID No.2. The invention further discloses an application of the multidrug-resistant efflux transporter gene OsMRET1 in regulation and control of chalkiness traits of rice. It is found for the first time that the gene OsMRET1 can regulate and control formation of chalkiness, an OsMRET1 gene knockout mutant is created through a gene editing technology, an OsMRET1 gene overexpression strain is obtained through a transgenic technology, it is verified that the OsMRET1 gene is a new gene for regulating and controlling rice chalkiness, and overexpression of the gene can reduce rice chalkiness and improve rice chalkiness. And a new gene resource and a technical route are provided for rice quality improvement.

The invention discloses a plant immunomodulatory gene RDP as well as an expression vector and application thereof. The inventor finds a plant immunomodulatory factor gene RDP from potatoes, after a corresponding sequence is obtained through cloning, agrobacterium competent cells are transferred into nicotiana benthamiana, it is verified that the plant immunomodulatory factor gene RDP can be successfully expressed in other plants, and it is verified that the pathogenic effects of various phytophthora are good. The gene is proved to be capable of effectively improving the disease resistance of the nicotiana benthamiana after being expressed. Through objective evaluation of an agrobacterium tumefaciens-mediated transient expression system, it is found that the plant immunomodulatory factor RDP can induce plants to generate resistance, the gene can be directly introduced into the plants through a transgenic technology due to the powerful function of the gene, disease-resistant varieties are developed and cultivated, and the gene has great significance in prevention and treatment of plant diseases.

The invention discloses application of a TaSPL18-5D protein and a related biological material thereof to regulation and control of the ear length and the yield of a plant. According to the invention, a TaSPL18-5D overexpression wheat strain is obtained through a transgenic technology, and then analysis on the TaSPL18-5D overexpression wheat strain finds that compared with wild type wheat KN199, the TaSPL18-5D overexpression wheat strain has the advantages that the ear length is shortened, and the spikelet number and the ear grain number are obviously reduced. Therefore, the TaSPL18-5D protein can negatively regulate the ear length, the spikelet number and the grain number per ear of the plant, a new target is provided for improving the plant type and the yield of the wheat after green revolution, and the TaSPL18-5D protein has important significance for cultivating high-yield wheat varieties in agricultural production.

The invention provides an application of a rape RPM1 interaction protein BnaRIN4 in regulating and controlling the oil content of rape seeds. On the basis of whole genome association analysis and whole transcriptome association analysis, a candidate gene BnaRIN4 for regulating and controlling the oil content is predicted, a homozygous mutant material with deletion of the gene BnaRIN4 function is further created through a transgenic technology, and the gene plays an important role in negative regulation and control of the oil content of the rape seeds, so that the application of the gene in negative regulation and control of the oil content of the rape seeds is realized. By specifically knocking out or mutating the gene, a novel rape germplasm of which the oil content of the seeds is remarkably improved can be obtained. The invention discloses the biological function of the gene in regulating and controlling the oil content of rape seeds for the first time, and provides theoretical basis and technical support for molecular breeding of high-oil-content rape varieties.

The invention belongs to the technical field of plant breeding in molecular biology, and provides a cotton GhVIP1 gene and application thereof in drought resistance and salt tolerance of plants. The nucleotide sequence of the GhVIP1 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the GhVIP1 gene is as shown in SEQ ID NO: 2. The gene is obviously up-regulated under the induction of salt and drought stress. An arabidopsis thaliana homozygous line overexpressed with the GhVIP1 gene is obtained through a transgenic technology, under the stress of drought and salt, the seed germination rate of the arabidopsis thaliana overexpressed with the gene is higher than that of a wild type, the growth condition of a transgenic line in the seedling stage is better than that of the wild type, the stem length, the total antioxidant capacity and the chlorophyll content are all superior to those of the wild type, and the MDA content is lower than that of the wild type; further, the drought resistance and the salt resistance of the arabidopsis thaliana are improved. When the gene is applied to drought-resistant and salt-resistant breeding of plants, the drought resistance and salt resistance of the plants can be improved, and an effective alternative scheme is provided for molecular breeding of drought-resistant and salt-resistant crops.

The invention relates to a DNA (Deoxyribose Nucleic Acid) for regulating and controlling a resistance gene LTPL160 of rice bacterial blight and breeding utilization of a protein of the DNA. According to the invention, the gene OsLTPL160 for regulating and controlling the resistance of the rice bacterial leaf blight is cloned by virtue of a non-gel quantitative proteomics analysis and transcription analysis method. The OsLTPL160 knockout and overexpression material is obtained by adopting a gene editing and transgenic technology. The OsLTPL160 is knocked out, so that the bacterial blight resistance of the rice is remarkably improved; and overexpression of the OsLTPL160 obviously reduces the resistance of the bacterial blight of the rice. The cloning of the OsLTPL160 gene and the construction of the material provide material and technical support for the bacterial blight resistance of the rice.

The application belongs to the technical field of genetic engineering, and particularly discloses AtDi19 Application of the gene in drought resistance and high-oil rape breeding AtDi19 The gene is first transferred into the rape by using the transgenic technology, and the transgenic rape obtained has significantly improved drought resistance compared with the wild type rape; and the oil content of the rape seed is significantly increased under the drought stress. Therefore, the application provides a new gene resource for drought-resistant and stable high-oil rape breeding.

The invention discloses application of a CsDDM1 gene in regulating and controlling the length of cucumber fruits, and relates to the technical field of bioengineering breeding. The nucleotide sequence of the CsDDM1 gene is as shown in SEQ ID NO: 1. The CsDDM1 gene participating in cucumber fruit length regulation and control is identified and verified, then a cucumber CsDDM1 gene knockout material is constructed by utilizing a transgenic technology, and function research shows that the cucumber fruit becomes shorter by knocking out the CsDDM1 gene, so that the mechanism blank of epigenetic regulation and control of the character is filled, the commodity and ornamental value of the cucumber are increased, and the cucumber CsDDM1 gene knockout material has good application prospects. The method solves the problems that traditional breeding is long in period and short fruit germplasm is deficient, breeding efficiency is improved, germplasm resources are enriched, the research result also provides a new thought for character improvement of other crops, and scientific and technological progress and innovative development of the whole field are promoted.

The invention discloses a gene for promoting low-nitrogen efficient utilization of rice and enlarging grains and application of the gene. The gene is derived from rice and is named as OsUGT, and the nucleotide sequence of the gene is shown as SEQ ID NO.1; the amino acid sequence of the protein coded by the gene is as shown in SEQ ID NO. 2. The invention provides a construction method of an OsUGT gene overexpressed transgenic rice strain, which comprises the following steps: constructing pRHVc-OsUGT recombinant plasmids, transforming the pRHVc-OsUGT recombinant plasmids into calluses through an agrobacterium tumefaciens-mediated method, and screening resistant calluses and regenerating plants to obtain the OsUGT gene overexpressed rice. The OsUGT gene is overexpressed in a rice plant by adopting a pRHVc vector through a transgenic technology, so that the nitrogen utilization efficiency of the plant in a medium-low nitrogen stress environment can be remarkably improved, grain enlargement and grain weight increase can be effectively promoted, stable improvement of the crop yield under a low-nitrogen condition is realized, and the technical effect is clear and efficient.

The invention discloses a transcription factor TgWRKY65 of torreya grandis as well as a coding gene and application of the transcription factor TgWRKY65. The amino acid sequence of the transcription factor TgWRKY65 is shown as SEQ ID NO.2, and the nucleotide sequence of the coding gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the transcription factor TgWRKY65. According to the invention, a homologous expression material is constructed through cloning of the TgWRKY65 gene, a transgenic technology and gene silencing, and the application prospect in the aspect of increasing the content of the torreya grandis catechin is great; when the coding gene is over-expressed, the content of catechin is increased; when the coding gene is silenced, the content of catechin is reduced.

The invention provides a highland barley HvDREB3 protein, a coding gene and application thereof, and belongs to the technical field of plant genetic engineering. The HvDREB3 protein provided by the invention has an amino acid sequence as shown in SEQ ID NO.1, and the coding gene of the HvDREB3 protein has a nucleotide sequence as shown in SEQ ID NO.2. The invention also provides application of the protein, the coding gene of the protein or a biological material containing the coding gene in regulation and control of plant freezing resistance, and a method for constructing an anti-freezing plant. The HvDREB3 gene is subjected to overexpression in highland barley through an overexpression transgenic technology, so that the survival rate is remarkably increased, the ion leakage rate is remarkably reduced, the chlorophyll content is increased, the antioxidant enzyme activity is enhanced, and the capability of resisting low-temperature freezing injury stress is remarkably improved.

The invention relates to the technical field of agricultural transgenosis, and particularly discloses application of a GhDBB28 gene in regulating and controlling salt tolerance of upland cotton plants, and the nucleotide sequence of the GhDBB28 gene is as shown in SEQ ID NO.1; the sensitivity of upland cotton plants to salt is improved by silencing the GhDBB28 gene. The activity of an anti-oxidation system in the upland cotton plant is reduced through silencing of GhDBB28, so that the plant is more sensitive to salt stress, and a scientific basis is provided for constructing a new plant type of a salt-sensitive transgenic upland cotton plant.

The invention discloses an application of a transcription factor ChZF7 of a basophilic microalgae Chlorella sp. BLD in regulation and control of salt tolerance of microalgae. According to the invention, the coding gene of the transcription factor ChZF7 is transferred into chlamydomonas reinhardtii by using a transgenic technology after intron insertion and codon optimization, so that transgenic chlamydomonas reinhardtii is obtained. The salt tolerance of the chlamydomonas reinhardtii with the over-expressed ChZF7 gene is remarkably improved, the accumulation of neutral fat and active oxygen under the stress of 225mM salt is less than that of the chlamydomonas reinhardtii in a control group, the content of penetrating substance proline is increased, in addition, the change of cell components in the chlamydomonas reinhardtii under the stress of salt can be weakened by the over-expressed ChZF7 gene, and the salt tolerance of the chlamydomonas reinhardtii is improved. The transcription factor ChZF7 and the gene coded by the transcription factor ChZF7 can regulate and control the salt tolerance of the chlamydomonas reinhardtii, a foundation is laid for genetic improvement and directed molecular breeding of the microalgae, the transcription factor ChZF7 plays an important role in improvement of the salt tolerance of the microalgae, reutilization of salinized water resources is facilitated, and the transcription factor ChZF7 and the gene coded by the transcription factor ChZF7 have wide application prospects.

The invention belongs to the technical field of biological detection, and particularly relates to an optimization method for evaluating the whitening effect of cosmetics through zebra fish and application. According to the method, fertilized zebrafish is grouped, temperature is controlled for a dark incubation period, the pH value of an administration solution is regulated and controlled, an image analysis technology, molecular biology, a transgenic technology and the like are combined to optimize a zebrafish-based cosmetic whitening effect evaluation system, and multi-index comprehensive evaluation is carried out. A rapid, reliable and ethical acceptable in-vivo evaluation scheme is provided for the whitening effect of the cosmetics; the method can be applied to screening and mechanism research of whitening active ingredients, and provides more scientific basis for product development.

The invention provides a medicago sativa ERF transcription factor MsERF076 and an application of the cold-resistant function of the medicago sativa ERF transcription factor MsERF076, and belongs to the technical field of genetic engineering. The invention provides a medicago sativa ERF transcription factor MsERF076. The amino acid sequence of the MsERF076 is as shown in SEQ ID NO. 13. The invention also provides a preparation method of the medicago sativa ERF transcription factor MsERF076. By adopting a transgenic technology, the MsERF076 gene is integrated into an alfalfa genome, so that MsERF076 is overexpressed in an alfalfa body, the internode number, the leaf number and the biomass of the alfalfa can be increased, and meanwhile, the cold resistance of the alfalfa can be remarkably improved. The transcription factor MsERF076 provided by the invention can promote the growth of medicago sativa, and also can improve the cold resistance of medicago sativa. The transcription factor MsERF076 provided by the invention provides a new theoretical basis and a candidate gene for cultivating a new variety of medicago sativa with high resistance, high yield and high protein.

The present application belongs to the field of genetic engineering technology in molecular biology, and relates to a gene for regulating sugar content of strawberry fruits and application thereof. The present application clones FveSMXL7 gene from forest strawberry, uses the FveSMXL7 gene to construct a silencing vector of the FveSMXL7 gene, and proves that reducing expression of the FveSMXL7 gene can increase the sugar content of strawberry fruits by stable transgenic technology, and indicates that the gene has the function of regulating the sugar content of strawberry fruits.

The invention provides application of a phellinus polyketide synthase gene SvPKS1 in promoting bioluminescence of fungi or plants, the coding sequence of the SvPKS1 is shown as SEQ ID NO.1, and the fungi are fungi for producing caffeic acid. According to the application, the SvPKS1 and other genes (nnH3H, nnLuz and nnCPH) of an FBP fungus luminescence pathway are co-expressed in fungi or plants by utilizing a transgenic technology, so that the fungi or plants are promoted to emit light biologically. The invention discloses co-expression of the phellinus polyketide synthase gene SvPKS1 and fungus luminescence pathway genes nnH3H, nnLuz and nnCPH for the first time, promotes fungus or plant bioluminescence, and provides a key candidate enzyme gene for promoting fungus or plant sustainable luminescence.

The invention relates to a rice gene OsMYB63L and application of the rice gene OsMYB63L in regulation and control of spike branching and yield, and belongs to the technical field of plant gene breeding, the nucleotide sequence of the gene OsMYB63L is as shown in SEQ ID NO.1, the amino acid sequence of protein coded by the gene is as shown in SEQ ID NO.2, overexpression of the gene OsMYB63L promotes development of the spike branching of rice, then the yield of rice is increased, a transgenic technology is utilized, and the yield of the rice is increased. Overexpression is carried out on the rice gene OsMYB63L, and a rice plant with increased ear branch number and increased yield can be obtained. The invention discovers and verifies that the rice gene OsMYB63L has the function of regulating and controlling the ear branching number and the yield of rice. And a new gene resource and a technical path are provided for molecular breeding of high-yield rice.

The application discloses application of MdERF77 gene in plant resistance to saline-alkali stress, and belongs to the technical field of biotechnology. The application overexpresses the MdERF77 gene in an apple plant through transgenic technology, significantly enhances the tolerance of the plant to saline-alkali stress, and has outstanding technical effects. The method can effectively improve the growth adaptability of the apple in a saline-alkali environment, so that the transgenic plant shows stronger antioxidant capacity, higher photosynthetic efficiency and more stable membrane system under the saline-alkali stress, thereby guaranteeing normal development and quality improvement of fruits, and reducing growth inhibition and yield loss caused by stress. The application provides a reference for improvement of stress resistance of fruit trees, and has a wide agricultural application prospect.

An apple stress-resistant gene MdRPP13-like-1 and its applications, the MdRPP13-like-1 The nucleotide sequence of the gene is shown in SEQ.ID.NO.1. Using transgenic technology driven by the 35S strong promoter, the gene will... MdRPP13-like-1 Gene overexpression vectors were transferred into apples to obtain transgenic apples. This invention is the first to improve plant resistance to continuous cropping using plant genetic engineering technology. A complete coding segment of a disease resistance-related gene was isolated and cloned from apple rootstock, and the function of this gene was verified. Its function was ultimately used to discover the optimal use of [the specific technology / method / approach]. MdRPP13-like-1 MdRPP13-like-1 Transgenic plants that were overexpressed in apples showed significantly improved resistance to continuous cropping.

The invention discloses a gene related to rice tillering as well as an encoding protein and application thereof, and belongs to the technical field of plant genetic engineering. According to the invention, a gene SSRP1 related to rice tillering is found, and a coding gene of the protein is reversely knocked out through a transgenic technology, so that dwarf multi-tillering transgenic rice is obtained. The tillering of the SSRP1 knockout plant is obviously increased and is 1.8 times of that of wild rice. The results prove that the SSRP1 gene participates in regulating and controlling the tiller number of rice, and can be applied to regulating the plant type and forming a reasonable plant population structure, so that the yield and quality of crops in unit area of cultivated land are improved.

The invention belongs to the technical field of gene engineering, and discloses a wheat stem rot resistance gene TaRBP3 and application thereof. According to the invention, the wheat stem rot resistance gene TaRBP3 is transferred into common wheat for overexpression by using a transgenic technology for the first time, and the effect of the TaRBP3 gene in wheat stem rot resistance variety breeding is verified, so that the genetic regulation mechanism of wheat stem rot resistance is disclosed; in addition, improvement of wheat varieties by using a genetic engineering technology is promoted, and meanwhile, an important theoretical foundation is laid for breeding of basal stem rot resistant wheat varieties.

The invention relates to the technical field of plant disease prevention and control and transgenosis, and mainly relates to a rice OsDRM2 gene and application thereof in resisting rice stripe virus diseases.

The invention discloses an application of a heat-resistant related gene Hsc70-4 of apis cerana and an encoding protein of the heat-resistant related gene Hsc70-4, and belongs to the technical field of cytobiology and molecular biology. The invention verifies that silent Hsc70-4 can reduce the survival rate of Chinese bees under heat stress, and overexpressed Hsc70-4 can increase the survival rate of drosophila S2 cells under heat stress. Induced expression of Hsc70-4 is helpful for reducing oxidative damage of heat stress to apis cerana and improving heat resistance of apis cerana. In view of the heat resistance of the Hsc70-4, overexpression of the Hsc70-4 in bees or economic animals is expected to be realized through a transgenic technology in the future, so that the heat resistance of the Hsc70-4 is improved, the yield of the Hsc70-4 under a heat stress condition is further improved, and a new gene target is provided for coping with ecological and agricultural challenges under a global warming background.

The invention discloses a stylosanthes guianensis purple acid phytase gene SgPAPhya and a stylosanthes guianensis purple acid phytase gene SgPAPhyb capable of enhancing the aluminum toxicity stress resistance of a plant and application of the stylosanthes guianensis purple acid phytase gene SgPAPhyb, and the aluminum toxicity stress resistance of the plant can be remarkably improved through a transgenic plant in which the SgPAPhya gene and the SgPAPhyb gene are excessively expressed; the SgPAPhya gene and the SgPAPhyb gene play an important role in improving the aluminum toxicity stress resistance of the plants, can be used for improving crops through a transgenic technology and improving the aluminum toxicity stress tolerance of the plants, and have important guiding significance for subsequent cultivation of aluminum toxicity stress resistant crop new germplasm suitable for being planted in acid soil.

The invention provides a gene for regulating and controlling the shape of a tomato leaf and application thereof, and a construction method of a recombinant expression vector is characterized in that an SlPUB26 gene is inserted into a vector containing a strong promoter by utilizing an overexpression transgenic technology. Transgenic plants with different gene expression level up-regulation multiples are obtained through agrobacterium tumefaciens-mediated transformation, so that single plants with different leaf phenotype change degrees are obtained. According to the method, a precise targeting sequence does not need to be designed, the miss-target hidden danger does not exist, and single plants with different SlPUB26 gene expression levels can be obtained only by detecting the gene expression quantity, so that the single plants with different leaf shape change degrees are obtained. The technical operation difficulty is low, and the method is more suitable for non-professional molecular breeding teams or grass-roots agricultural scientific research institutions. The higher the up-regulation multiple of the expression level of the SlPUB26 gene is, the lower the length-width ratio of the tomato leaf is, so that the shape of the tomato leaf can be quickly and accurately adjusted by adjusting the expression level of the SlPUB26 gene.

The present invention discloses a banana promoter proMbU5 and its application. The nucleotide sequence of the promoter proMbU5 is shown in SEQ ID NO: 1. The present invention amplifies a promoter of a Ubiquitin gene by PCR, named proMbU5, and constructs a plant expression vector containing a GUS reporter gene. Then, it is transformed into rice by the Agrobacterium method. The GUS staining experiment of the transformed seedlings shows that the promoter proMbU5 has a strong promoter efficiency and can efficiently promote the expression of the GUS gene in rice. Rice is a model plant for C3 crops among monocotyledons. The promoter proMbU5 can provide a new and efficient promoter option for transgenic research of monocotyledons, and can be applied to more transgenic technology fields of monocotyledons.

The application discloses a Phyllostachys edulis-derived PeDHN2 gene and application of a coded protein thereof, and belongs to the technical field of plant biological breeding. A nucleotide sequence of the PeDHN2 gene is shown as SEQ ID NO. 11; and a sequence of the coded protein of the PeDHN2 gene is shown as SEQ ID NO. 12. The PeDHN2 gene is cloned from the Phyllostachys edulis, and experiments show that the gene is significantly up-regulated under salt stress, drought and abscisic acid (ABA) stress, and after being introduced into rice through a transgenic technology, the germination rate and root growth ability of the rice under salt stress are significantly improved. The application provides a key gene resource and technical support for molecular design breeding of salt-tolerant crops.