229 results about "Transgenic technology" patented technology

The invention belongs to the technical field of biology, and particularly relates to application of a soybean GmSRO5 gene in promoting generation of leguminous plant root nodules. A pZP211 overexpression vector is utilized, a transgenic plant is rapidly obtained through agrobacterium tumefaciens-mediated soybean genetic transformation, GmSRO5 is successfully overexpressed, root nodule number statistics is carried out on a chimera transgenic plant, and a result shows that the GmSRO5 gene can positively regulate and control the number of soybean root nodules and has a remarkable effect. The invention proposes that SRO5 can positively regulate and control the soybean nodulation process for the first time, and proves that the soybean GmSRO5 gene overexpression can significantly increase the number of soybean root nodules, and the soybean GmSRO5 gene overexpression can be applied to soybean production and molecular breeding through transgenosis, molecular marking and other means in the future, and has very important application value.

The invention discloses a gene CsIAA4 for improving parthenocarpy capacity of cucumbers and application of the gene CsIAA4, and identifies a novel parthenocarpy regulating gene CsIAA4 of the cucumbers, and the nucleotide sequence of the gene CsIAA4 is as shown in SEQ ID NO.1. The parthenocarpy ability of the gene editing mutant is weakened, and the parthenocarpy ability of the overexpression mutant is remarkably enhanced. The gene CsIAA4 can be used for guiding breeding and improvement of cucumber strong parthenocarpy germplasm resources, and technical support is provided for breeding of cucumber parthenocarpy varieties. The application of the CsIAA4 in cucumber parthenocarpy is jointly explored through two transgenic technologies, a new gene resource is provided for genetic improvement of cucumber parthenocarpy molecules, the cucumber parthenocarpy gene CsIAA4 is provided, and the cucumber parthenocarpy gene CsIAA4 has important application value in the aspect of research and improvement of cucumber parthenocarpy capacity.

The invention relates to the technical field of plant genetic engineering, and discloses application of a soybean low-phosphorus-tolerant gene GmPP2-10 in promoting growth of plants in a low-phosphorus stress environment. Researches show that the expression of the GmPP2-10 gene is increased under the induction of low phosphorus stress, and under different phosphorus concentration treatment conditions, the over-expression of the GmPP2-10 can increase the biomass of a transgenic plant and promote the growth of the plant under the low phosphorus condition; meanwhile, overexpression of GmPP2-10 can improve the tolerance of plants to low-phosphorus stress, and reduce the inhibition effect of low phosphorus on plant root growth. Therefore, the GmPP2-10 plays an important role in adapting to the low-phosphorus stress of the plant, and the adaptive capacity of the plant to the low-phosphorus stress of the acid soil can be improved by transferring the GmPP2-10 into the plant through a transgenic technology.

The invention discloses an effect protein E56, a coding gene and application of the effect protein E56 to improvement of southern rust resistance of corn, and belongs to the technical field of agricultural biology. The amino acid sequence of the effector protein E56 is as shown in SEQ ID NO. 1. A reverse genetics method is utilized to analyze the Puccinia polystachys effect protein E56 gene, and the E56 gene is up-regulated in expression in the Puccinia polystachys infection process. The E56 gene is silenced by adopting a host-mediated gene silencing technology, and the toxic function of the E56 gene in the infection process of the southern rust disease is determined. A specific fragment of the E56 gene is cloned to an RNAi interference vector, a corn immature embryo is transformed by using an agrobacterium tumefaciens-mediated transgenic technology, and an obtained transgenic corn plant shows resistance to the corn southern rust disease. The E56-RNAi transgenic plant shows resistance to puccinia polypoda, so that the effect protein E56 can be used for creating a new strain for resisting the southern rust disease.

The invention discloses an application of a multidrug-resistant efflux transporter gene OsMRET1 in regulation and control of chalkiness traits of rice. A nucleotide sequence of a coding region of the gene is shown as SEQ ID No.1, and a coded amino acid sequence is shown as SEQ ID No.2. The invention further discloses an application of the multidrug-resistant efflux transporter gene OsMRET1 in regulation and control of chalkiness traits of rice. It is found for the first time that the gene OsMRET1 can regulate and control formation of chalkiness, an OsMRET1 gene knockout mutant is created through a gene editing technology, an OsMRET1 gene overexpression strain is obtained through a transgenic technology, it is verified that the OsMRET1 gene is a new gene for regulating and controlling rice chalkiness, and overexpression of the gene can reduce rice chalkiness and improve rice chalkiness. And a new gene resource and a technical route are provided for rice quality improvement.

The invention discloses a plant immunomodulatory gene RDP as well as an expression vector and application thereof. The inventor finds a plant immunomodulatory factor gene RDP from potatoes, after a corresponding sequence is obtained through cloning, agrobacterium competent cells are transferred into nicotiana benthamiana, it is verified that the plant immunomodulatory factor gene RDP can be successfully expressed in other plants, and it is verified that the pathogenic effects of various phytophthora are good. The gene is proved to be capable of effectively improving the disease resistance of the nicotiana benthamiana after being expressed. Through objective evaluation of an agrobacterium tumefaciens-mediated transient expression system, it is found that the plant immunomodulatory factor RDP can induce plants to generate resistance, the gene can be directly introduced into the plants through a transgenic technology due to the powerful function of the gene, disease-resistant varieties are developed and cultivated, and the gene has great significance in prevention and treatment of plant diseases.

The invention relates to the technical field of plant tissue culture and plant transgenosis, in particular to a method for co-transformation of an LcSUCLA alpha-LcSUCLA beta-pEGFC binary expression vector and Leymus chinensis double genes. The method comprises the following steps: obtaining leymus chinensis tissue culture sterile calluses, transforming agrobacterium competent by a freeze thawing method, activating agrobacterium tumefaciens, infecting the leymus chinensis calluses and co-culturing, screening and culturing plant transformation materials, differentiating and screening a culture medium and culture conditions, and differentiating and screening a culture medium and culture conditions as well as a rooting culture medium and culture conditions. According to the invention, double-gene co-transformation of leymus chinensis is realized, and a leymus chinensis double-transgenic plant is obtained. The cotransformation of the LcSUCLA alpha and the LcSUCLA beta in the leymus chinensis can synthesize more succinic acid than the single transformation, so that the tolerance of the leymus chinensis under the stress of basic salt is further improved.

The invention relates to the technical field of plant genetic engineering, and discloses a plant low-phosphorus-resistant important gene GsMYB7 and application thereof. According to the invention, an MYB family GsMYB7 gene is cloned in soybean for the first time. Researches show that the expression of the GsMYB7 gene is increased under the induction of low phosphorus stress, and under different phosphorus concentration treatment conditions, the overexpression GsMYB7 can obviously increase the biomass of transgenic plants and promote the growth of the plants under the low phosphorus condition; meanwhile, overexpression of GsMYB7 can improve the tolerance of plants to low-phosphorus stress, and reduce the inhibition effect of low phosphorus on plant root growth. Therefore, the GsMYB7 plays an important role in adapting to the low-phosphorus stress of the plant, and the adaptive capacity of the plant to the low-phosphorus stress of the acid soil can be improved through a transgenic technology.

The invention discloses application of a TaSPL18-5D protein and a related biological material thereof to regulation and control of the ear length and the yield of a plant. According to the invention, a TaSPL18-5D overexpression wheat strain is obtained through a transgenic technology, and then analysis on the TaSPL18-5D overexpression wheat strain finds that compared with wild type wheat KN199, the TaSPL18-5D overexpression wheat strain has the advantages that the ear length is shortened, and the spikelet number and the ear grain number are obviously reduced. Therefore, the TaSPL18-5D protein can negatively regulate the ear length, the spikelet number and the grain number per ear of the plant, a new target is provided for improving the plant type and the yield of the wheat after green revolution, and the TaSPL18-5D protein has important significance for cultivating high-yield wheat varieties in agricultural production.

The invention provides an application of a rape RPM1 interaction protein BnaRIN4 in regulating and controlling the oil content of rape seeds. On the basis of whole genome association analysis and whole transcriptome association analysis, a candidate gene BnaRIN4 for regulating and controlling the oil content is predicted, a homozygous mutant material with deletion of the gene BnaRIN4 function is further created through a transgenic technology, and the gene plays an important role in negative regulation and control of the oil content of the rape seeds, so that the application of the gene in negative regulation and control of the oil content of the rape seeds is realized. By specifically knocking out or mutating the gene, a novel rape germplasm of which the oil content of the seeds is remarkably improved can be obtained. The invention discloses the biological function of the gene in regulating and controlling the oil content of rape seeds for the first time, and provides theoretical basis and technical support for molecular breeding of high-oil-content rape varieties.

The invention belongs to the technical field of plant breeding in molecular biology, and provides a cotton GhVIP1 gene and application thereof in drought resistance and salt tolerance of plants. The nucleotide sequence of the GhVIP1 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the GhVIP1 gene is as shown in SEQ ID NO: 2. The gene is obviously up-regulated under the induction of salt and drought stress. An arabidopsis thaliana homozygous line overexpressed with the GhVIP1 gene is obtained through a transgenic technology, under the stress of drought and salt, the seed germination rate of the arabidopsis thaliana overexpressed with the gene is higher than that of a wild type, the growth condition of a transgenic line in the seedling stage is better than that of the wild type, the stem length, the total antioxidant capacity and the chlorophyll content are all superior to those of the wild type, and the MDA content is lower than that of the wild type; further, the drought resistance and the salt resistance of the arabidopsis thaliana are improved. When the gene is applied to drought-resistant and salt-resistant breeding of plants, the drought resistance and salt resistance of the plants can be improved, and an effective alternative scheme is provided for molecular breeding of drought-resistant and salt-resistant crops.

The invention relates to a DNA (Deoxyribose Nucleic Acid) for regulating and controlling a resistance gene LTPL160 of rice bacterial blight and breeding utilization of a protein of the DNA. According to the invention, the gene OsLTPL160 for regulating and controlling the resistance of the rice bacterial leaf blight is cloned by virtue of a non-gel quantitative proteomics analysis and transcription analysis method. The OsLTPL160 knockout and overexpression material is obtained by adopting a gene editing and transgenic technology. The OsLTPL160 is knocked out, so that the bacterial blight resistance of the rice is remarkably improved; and overexpression of the OsLTPL160 obviously reduces the resistance of the bacterial blight of the rice. The cloning of the OsLTPL160 gene and the construction of the material provide material and technical support for the bacterial blight resistance of the rice.

The application belongs to the technical field of genetic engineering, and particularly discloses AtDi19 Application of the gene in drought resistance and high-oil rape breeding AtDi19 The gene is first transferred into the rape by using the transgenic technology, and the transgenic rape obtained has significantly improved drought resistance compared with the wild type rape; and the oil content of the rape seed is significantly increased under the drought stress. Therefore, the application provides a new gene resource for drought-resistant and stable high-oil rape breeding.

The invention discloses application of a CsDDM1 gene in regulating and controlling the length of cucumber fruits, and relates to the technical field of bioengineering breeding. The nucleotide sequence of the CsDDM1 gene is as shown in SEQ ID NO: 1. The CsDDM1 gene participating in cucumber fruit length regulation and control is identified and verified, then a cucumber CsDDM1 gene knockout material is constructed by utilizing a transgenic technology, and function research shows that the cucumber fruit becomes shorter by knocking out the CsDDM1 gene, so that the mechanism blank of epigenetic regulation and control of the character is filled, the commodity and ornamental value of the cucumber are increased, and the cucumber CsDDM1 gene knockout material has good application prospects. The method solves the problems that traditional breeding is long in period and short fruit germplasm is deficient, breeding efficiency is improved, germplasm resources are enriched, the research result also provides a new thought for character improvement of other crops, and scientific and technological progress and innovative development of the whole field are promoted.

The invention discloses a gene for promoting low-nitrogen efficient utilization of rice and enlarging grains and application of the gene. The gene is derived from rice and is named as OsUGT, and the nucleotide sequence of the gene is shown as SEQ ID NO.1; the amino acid sequence of the protein coded by the gene is as shown in SEQ ID NO. 2. The invention provides a construction method of an OsUGT gene overexpressed transgenic rice strain, which comprises the following steps: constructing pRHVc-OsUGT recombinant plasmids, transforming the pRHVc-OsUGT recombinant plasmids into calluses through an agrobacterium tumefaciens-mediated method, and screening resistant calluses and regenerating plants to obtain the OsUGT gene overexpressed rice. The OsUGT gene is overexpressed in a rice plant by adopting a pRHVc vector through a transgenic technology, so that the nitrogen utilization efficiency of the plant in a medium-low nitrogen stress environment can be remarkably improved, grain enlargement and grain weight increase can be effectively promoted, stable improvement of the crop yield under a low-nitrogen condition is realized, and the technical effect is clear and efficient.

The invention discloses a gene OsNST2 for transporting nucleotide sugar and application of the gene OsNST2. A nucleotide sequence of a coding region of the gene is as shown in SEQ ID No. 1, and an amino acid sequence coded by the gene is as shown in SEQ ID No. 2. It is found for the first time that the growth, development and yield of rice are affected by the OsNST2 by regulating and controlling formation of rice cell walls, an OsNST2 gene knock-down strain is created through an RNAi technology, an OsNST2 gene overexpression strain is obtained through an overexpression transgenic technology, it is verified that the OsNST2 gene is a new gene for regulating and controlling formation of the rice cell walls, and a new gene resource is provided for high yield of rice.

The invention discloses a transcription factor TgWRKY65 of torreya grandis as well as a coding gene and application of the transcription factor TgWRKY65. The amino acid sequence of the transcription factor TgWRKY65 is shown as SEQ ID NO.2, and the nucleotide sequence of the coding gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the transcription factor TgWRKY65. According to the invention, a homologous expression material is constructed through cloning of the TgWRKY65 gene, a transgenic technology and gene silencing, and the application prospect in the aspect of increasing the content of the torreya grandis catechin is great; when the coding gene is over-expressed, the content of catechin is increased; when the coding gene is silenced, the content of catechin is reduced.

The invention provides a highland barley HvDREB3 protein, a coding gene and application thereof, and belongs to the technical field of plant genetic engineering. The HvDREB3 protein provided by the invention has an amino acid sequence as shown in SEQ ID NO.1, and the coding gene of the HvDREB3 protein has a nucleotide sequence as shown in SEQ ID NO.2. The invention also provides application of the protein, the coding gene of the protein or a biological material containing the coding gene in regulation and control of plant freezing resistance, and a method for constructing an anti-freezing plant. The HvDREB3 gene is subjected to overexpression in highland barley through an overexpression transgenic technology, so that the survival rate is remarkably increased, the ion leakage rate is remarkably reduced, the chlorophyll content is increased, the antioxidant enzyme activity is enhanced, and the capability of resisting low-temperature freezing injury stress is remarkably improved.

The invention discloses an application of a related gene Zm00001eb129860 in the flowering period of corn in regulation and control of flowering transformation. According to the invention, a high-expression gene Zm00001eb129860 related to the flowering period of corn is screened from transcriptome data of inflorescence meristem in a key period of flowering transformation of corn, the gene affects the change process of the flowering transformation of corn, and a gene knockout mutant plant has a late flowering phenotype; when the gene is over-expressed in a corn plant, a transgenic plant with early flowering transformation can be obtained, so that the flowering period of the corn is shortened, genetic improvement and breeding of the plant are facilitated, and the gene has important significance for promoting early flowering of the corn. It is indicated that Zm00001eb129860 plays an important role in advancing corn flowering transformation, can be used for regulating and controlling the flowering period of plants through a transgenic technology, facilitates cultivation of early flowering plants and facilitates genetic improvement of the plants.

The invention relates to the technical field of agricultural transgenosis, and particularly discloses application of a GhDBB28 gene in regulating and controlling salt tolerance of upland cotton plants, and the nucleotide sequence of the GhDBB28 gene is as shown in SEQ ID NO.1; the sensitivity of upland cotton plants to salt is improved by silencing the GhDBB28 gene. The activity of an anti-oxidation system in the upland cotton plant is reduced through silencing of GhDBB28, so that the plant is more sensitive to salt stress, and a scientific basis is provided for constructing a new plant type of a salt-sensitive transgenic upland cotton plant.

The invention discloses an application of a transcription factor ChZF7 of a basophilic microalgae Chlorella sp. BLD in regulation and control of salt tolerance of microalgae. According to the invention, the coding gene of the transcription factor ChZF7 is transferred into chlamydomonas reinhardtii by using a transgenic technology after intron insertion and codon optimization, so that transgenic chlamydomonas reinhardtii is obtained. The salt tolerance of the chlamydomonas reinhardtii with the over-expressed ChZF7 gene is remarkably improved, the accumulation of neutral fat and active oxygen under the stress of 225mM salt is less than that of the chlamydomonas reinhardtii in a control group, the content of penetrating substance proline is increased, in addition, the change of cell components in the chlamydomonas reinhardtii under the stress of salt can be weakened by the over-expressed ChZF7 gene, and the salt tolerance of the chlamydomonas reinhardtii is improved. The transcription factor ChZF7 and the gene coded by the transcription factor ChZF7 can regulate and control the salt tolerance of the chlamydomonas reinhardtii, a foundation is laid for genetic improvement and directed molecular breeding of the microalgae, the transcription factor ChZF7 plays an important role in improvement of the salt tolerance of the microalgae, reutilization of salinized water resources is facilitated, and the transcription factor ChZF7 and the gene coded by the transcription factor ChZF7 have wide application prospects.

The invention belongs to the technical field of biological detection, and particularly relates to an optimization method for evaluating the whitening effect of cosmetics through zebra fish and application. According to the method, fertilized zebrafish is grouped, temperature is controlled for a dark incubation period, the pH value of an administration solution is regulated and controlled, an image analysis technology, molecular biology, a transgenic technology and the like are combined to optimize a zebrafish-based cosmetic whitening effect evaluation system, and multi-index comprehensive evaluation is carried out. A rapid, reliable and ethical acceptable in-vivo evaluation scheme is provided for the whitening effect of the cosmetics; the method can be applied to screening and mechanism research of whitening active ingredients, and provides more scientific basis for product development.

The invention provides a medicago sativa ERF transcription factor MsERF076 and an application of the cold-resistant function of the medicago sativa ERF transcription factor MsERF076, and belongs to the technical field of genetic engineering. The invention provides a medicago sativa ERF transcription factor MsERF076. The amino acid sequence of the MsERF076 is as shown in SEQ ID NO. 13. The invention also provides a preparation method of the medicago sativa ERF transcription factor MsERF076. By adopting a transgenic technology, the MsERF076 gene is integrated into an alfalfa genome, so that MsERF076 is overexpressed in an alfalfa body, the internode number, the leaf number and the biomass of the alfalfa can be increased, and meanwhile, the cold resistance of the alfalfa can be remarkably improved. The transcription factor MsERF076 provided by the invention can promote the growth of medicago sativa, and also can improve the cold resistance of medicago sativa. The transcription factor MsERF076 provided by the invention provides a new theoretical basis and a candidate gene for cultivating a new variety of medicago sativa with high resistance, high yield and high protein.

The present application belongs to the field of genetic engineering technology in molecular biology, and relates to a gene for regulating sugar content of strawberry fruits and application thereof. The present application clones FveSMXL7 gene from forest strawberry, uses the FveSMXL7 gene to construct a silencing vector of the FveSMXL7 gene, and proves that reducing expression of the FveSMXL7 gene can increase the sugar content of strawberry fruits by stable transgenic technology, and indicates that the gene has the function of regulating the sugar content of strawberry fruits.

The present invention discloses the application of the rice OsHsfC1a gene in regulating the heading stage of rice. By mutating the rice OsHsfC1a gene, it is found that inhibiting the expression of the OsHsfC1a gene can prolong the heading stage of rice, while overexpressing the OsHsfC1a gene can shorten the heading stage of rice. At the same time, mutating or overexpressing the OsHsfC1a gene does not reduce the rice yield. Therefore, the OsHsfC1a gene can be used as an effective target for rice genetic breeding to construct mutant rice with delayed or advanced growth periods. In addition, the method of using the CRISPR / Cas9 technology to mutate the OsHsfC1a gene to obtain mutants with slightly delayed growth periods provides an effective approach for rice design breeding, which has stronger purposefulness, less damage to the genome, and can avoid the possible risks brought by transgenic technology compared with mutants obtained by chemical and physical mutagenesis.

The invention provides application of a phellinus polyketide synthase gene SvPKS1 in promoting bioluminescence of fungi or plants, the coding sequence of the SvPKS1 is shown as SEQ ID NO.1, and the fungi are fungi for producing caffeic acid. According to the application, the SvPKS1 and other genes (nnH3H, nnLuz and nnCPH) of an FBP fungus luminescence pathway are co-expressed in fungi or plants by utilizing a transgenic technology, so that the fungi or plants are promoted to emit light biologically. The invention discloses co-expression of the phellinus polyketide synthase gene SvPKS1 and fungus luminescence pathway genes nnH3H, nnLuz and nnCPH for the first time, promotes fungus or plant bioluminescence, and provides a key candidate enzyme gene for promoting fungus or plant sustainable luminescence.

The invention relates to a rice gene OsMYB63L and application of the rice gene OsMYB63L in regulation and control of spike branching and yield, and belongs to the technical field of plant gene breeding, the nucleotide sequence of the gene OsMYB63L is as shown in SEQ ID NO.1, the amino acid sequence of protein coded by the gene is as shown in SEQ ID NO.2, overexpression of the gene OsMYB63L promotes development of the spike branching of rice, then the yield of rice is increased, a transgenic technology is utilized, and the yield of the rice is increased. Overexpression is carried out on the rice gene OsMYB63L, and a rice plant with increased ear branch number and increased yield can be obtained. The invention discovers and verifies that the rice gene OsMYB63L has the function of regulating and controlling the ear branching number and the yield of rice. And a new gene resource and a technical path are provided for molecular breeding of high-yield rice.

The application discloses application of MdERF77 gene in plant resistance to saline-alkali stress, and belongs to the technical field of biotechnology. The application overexpresses the MdERF77 gene in an apple plant through transgenic technology, significantly enhances the tolerance of the plant to saline-alkali stress, and has outstanding technical effects. The method can effectively improve the growth adaptability of the apple in a saline-alkali environment, so that the transgenic plant shows stronger antioxidant capacity, higher photosynthetic efficiency and more stable membrane system under the saline-alkali stress, thereby guaranteeing normal development and quality improvement of fruits, and reducing growth inhibition and yield loss caused by stress. The application provides a reference for improvement of stress resistance of fruit trees, and has a wide agricultural application prospect.

The invention discloses application of a GE gene in regulating and controlling the number of regenerated buds of rice callus, and belongs to the technical field of plant genetic engineering. The invention finds a key regulatory factor GE for regulating and controlling the regeneration of the rice callus. Through construction of the GE knockout mutant and the overexpression strain, compared with a wild type, the quantity of callus regeneration buds of the GE knockout mutant is remarkably reduced, and the quantity of callus regeneration buds of the overexpression strain is remarkably increased. Therefore, it is determined that the GE gene plays an important role in regulating and controlling the regeneration process of the callus. Therefore, the regeneration capacity of the callus can be improved by overexpressing the GE gene, so that the transgenosis efficiency is improved.