94 results about "Transgenic technology" patented technology

The invention discloses a plant immunomodulatory gene RDP as well as an expression vector and application thereof. The inventor finds a plant immunomodulatory factor gene RDP from potatoes, after a corresponding sequence is obtained through cloning, agrobacterium competent cells are transferred into nicotiana benthamiana, it is verified that the plant immunomodulatory factor gene RDP can be successfully expressed in other plants, and it is verified that the pathogenic effects of various phytophthora are good. The gene is proved to be capable of effectively improving the disease resistance of the nicotiana benthamiana after being expressed. Through objective evaluation of an agrobacterium tumefaciens-mediated transient expression system, it is found that the plant immunomodulatory factor RDP can induce plants to generate resistance, the gene can be directly introduced into the plants through a transgenic technology due to the powerful function of the gene, disease-resistant varieties are developed and cultivated, and the gene has great significance in prevention and treatment of plant diseases.

The invention discloses application of a TaSPL18-5D protein and a related biological material thereof to regulation and control of the ear length and the yield of a plant. According to the invention, a TaSPL18-5D overexpression wheat strain is obtained through a transgenic technology, and then analysis on the TaSPL18-5D overexpression wheat strain finds that compared with wild type wheat KN199, the TaSPL18-5D overexpression wheat strain has the advantages that the ear length is shortened, and the spikelet number and the ear grain number are obviously reduced. Therefore, the TaSPL18-5D protein can negatively regulate the ear length, the spikelet number and the grain number per ear of the plant, a new target is provided for improving the plant type and the yield of the wheat after green revolution, and the TaSPL18-5D protein has important significance for cultivating high-yield wheat varieties in agricultural production.

The application belongs to the technical field of genetic engineering, and particularly discloses AtDi19 Application of the gene in drought resistance and high-oil rape breeding AtDi19 The gene is first transferred into the rape by using the transgenic technology, and the transgenic rape obtained has significantly improved drought resistance compared with the wild type rape; and the oil content of the rape seed is significantly increased under the drought stress. Therefore, the application provides a new gene resource for drought-resistant and stable high-oil rape breeding.

The invention discloses a gene for promoting low-nitrogen efficient utilization of rice and enlarging grains and application of the gene. The gene is derived from rice and is named as OsUGT, and the nucleotide sequence of the gene is shown as SEQ ID NO.1; the amino acid sequence of the protein coded by the gene is as shown in SEQ ID NO. 2. The invention provides a construction method of an OsUGT gene overexpressed transgenic rice strain, which comprises the following steps: constructing pRHVc-OsUGT recombinant plasmids, transforming the pRHVc-OsUGT recombinant plasmids into calluses through an agrobacterium tumefaciens-mediated method, and screening resistant calluses and regenerating plants to obtain the OsUGT gene overexpressed rice. The OsUGT gene is overexpressed in a rice plant by adopting a pRHVc vector through a transgenic technology, so that the nitrogen utilization efficiency of the plant in a medium-low nitrogen stress environment can be remarkably improved, grain enlargement and grain weight increase can be effectively promoted, stable improvement of the crop yield under a low-nitrogen condition is realized, and the technical effect is clear and efficient.

The invention discloses a transcription factor TgWRKY65 of torreya grandis as well as a coding gene and application of the transcription factor TgWRKY65. The amino acid sequence of the transcription factor TgWRKY65 is shown as SEQ ID NO.2, and the nucleotide sequence of the coding gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the transcription factor TgWRKY65. According to the invention, a homologous expression material is constructed through cloning of the TgWRKY65 gene, a transgenic technology and gene silencing, and the application prospect in the aspect of increasing the content of the torreya grandis catechin is great; when the coding gene is over-expressed, the content of catechin is increased; when the coding gene is silenced, the content of catechin is reduced.

The invention provides a highland barley HvDREB3 protein, a coding gene and application thereof, and belongs to the technical field of plant genetic engineering. The HvDREB3 protein provided by the invention has an amino acid sequence as shown in SEQ ID NO.1, and the coding gene of the HvDREB3 protein has a nucleotide sequence as shown in SEQ ID NO.2. The invention also provides application of the protein, the coding gene of the protein or a biological material containing the coding gene in regulation and control of plant freezing resistance, and a method for constructing an anti-freezing plant. The HvDREB3 gene is subjected to overexpression in highland barley through an overexpression transgenic technology, so that the survival rate is remarkably increased, the ion leakage rate is remarkably reduced, the chlorophyll content is increased, the antioxidant enzyme activity is enhanced, and the capability of resisting low-temperature freezing injury stress is remarkably improved.

The invention relates to the technical field of agricultural transgenosis, and particularly discloses application of a GhDBB28 gene in regulating and controlling salt tolerance of upland cotton plants, and the nucleotide sequence of the GhDBB28 gene is as shown in SEQ ID NO.1; the sensitivity of upland cotton plants to salt is improved by silencing the GhDBB28 gene. The activity of an anti-oxidation system in the upland cotton plant is reduced through silencing of GhDBB28, so that the plant is more sensitive to salt stress, and a scientific basis is provided for constructing a new plant type of a salt-sensitive transgenic upland cotton plant.

The invention belongs to the technical field of biological detection, and particularly relates to an optimization method for evaluating the whitening effect of cosmetics through zebra fish and application. According to the method, fertilized zebrafish is grouped, temperature is controlled for a dark incubation period, the pH value of an administration solution is regulated and controlled, an image analysis technology, molecular biology, a transgenic technology and the like are combined to optimize a zebrafish-based cosmetic whitening effect evaluation system, and multi-index comprehensive evaluation is carried out. A rapid, reliable and ethical acceptable in-vivo evaluation scheme is provided for the whitening effect of the cosmetics; the method can be applied to screening and mechanism research of whitening active ingredients, and provides more scientific basis for product development.

The present application belongs to the field of genetic engineering technology in molecular biology, and relates to a gene for regulating sugar content of strawberry fruits and application thereof. The present application clones FveSMXL7 gene from forest strawberry, uses the FveSMXL7 gene to construct a silencing vector of the FveSMXL7 gene, and proves that reducing expression of the FveSMXL7 gene can increase the sugar content of strawberry fruits by stable transgenic technology, and indicates that the gene has the function of regulating the sugar content of strawberry fruits.

The invention provides application of a phellinus polyketide synthase gene SvPKS1 in promoting bioluminescence of fungi or plants, the coding sequence of the SvPKS1 is shown as SEQ ID NO.1, and the fungi are fungi for producing caffeic acid. According to the application, the SvPKS1 and other genes (nnH3H, nnLuz and nnCPH) of an FBP fungus luminescence pathway are co-expressed in fungi or plants by utilizing a transgenic technology, so that the fungi or plants are promoted to emit light biologically. The invention discloses co-expression of the phellinus polyketide synthase gene SvPKS1 and fungus luminescence pathway genes nnH3H, nnLuz and nnCPH for the first time, promotes fungus or plant bioluminescence, and provides a key candidate enzyme gene for promoting fungus or plant sustainable luminescence.

The invention relates to a rice gene OsMYB63L and application of the rice gene OsMYB63L in regulation and control of spike branching and yield, and belongs to the technical field of plant gene breeding, the nucleotide sequence of the gene OsMYB63L is as shown in SEQ ID NO.1, the amino acid sequence of protein coded by the gene is as shown in SEQ ID NO.2, overexpression of the gene OsMYB63L promotes development of the spike branching of rice, then the yield of rice is increased, a transgenic technology is utilized, and the yield of the rice is increased. Overexpression is carried out on the rice gene OsMYB63L, and a rice plant with increased ear branch number and increased yield can be obtained. The invention discovers and verifies that the rice gene OsMYB63L has the function of regulating and controlling the ear branching number and the yield of rice. And a new gene resource and a technical path are provided for molecular breeding of high-yield rice.

The application discloses application of MdERF77 gene in plant resistance to saline-alkali stress, and belongs to the technical field of biotechnology. The application overexpresses the MdERF77 gene in an apple plant through transgenic technology, significantly enhances the tolerance of the plant to saline-alkali stress, and has outstanding technical effects. The method can effectively improve the growth adaptability of the apple in a saline-alkali environment, so that the transgenic plant shows stronger antioxidant capacity, higher photosynthetic efficiency and more stable membrane system under the saline-alkali stress, thereby guaranteeing normal development and quality improvement of fruits, and reducing growth inhibition and yield loss caused by stress. The application provides a reference for improvement of stress resistance of fruit trees, and has a wide agricultural application prospect.

An apple stress-resistant gene MdRPP13-like-1 and its applications, the MdRPP13-like-1 The nucleotide sequence of the gene is shown in SEQ.ID.NO.1. Using transgenic technology driven by the 35S strong promoter, the gene will... MdRPP13-like-1 Gene overexpression vectors were transferred into apples to obtain transgenic apples. This invention is the first to improve plant resistance to continuous cropping using plant genetic engineering technology. A complete coding segment of a disease resistance-related gene was isolated and cloned from apple rootstock, and the function of this gene was verified. Its function was ultimately used to discover the optimal use of [the specific technology / method / approach]. MdRPP13-like-1 MdRPP13-like-1 Transgenic plants that were overexpressed in apples showed significantly improved resistance to continuous cropping.

The invention discloses a gene related to rice tillering as well as an encoding protein and application thereof, and belongs to the technical field of plant genetic engineering. According to the invention, a gene SSRP1 related to rice tillering is found, and a coding gene of the protein is reversely knocked out through a transgenic technology, so that dwarf multi-tillering transgenic rice is obtained. The tillering of the SSRP1 knockout plant is obviously increased and is 1.8 times of that of wild rice. The results prove that the SSRP1 gene participates in regulating and controlling the tiller number of rice, and can be applied to regulating the plant type and forming a reasonable plant population structure, so that the yield and quality of crops in unit area of cultivated land are improved.

The invention belongs to the technical field of gene engineering, and discloses a wheat stem rot resistance gene TaRBP3 and application thereof. According to the invention, the wheat stem rot resistance gene TaRBP3 is transferred into common wheat for overexpression by using a transgenic technology for the first time, and the effect of the TaRBP3 gene in wheat stem rot resistance variety breeding is verified, so that the genetic regulation mechanism of wheat stem rot resistance is disclosed; in addition, improvement of wheat varieties by using a genetic engineering technology is promoted, and meanwhile, an important theoretical foundation is laid for breeding of basal stem rot resistant wheat varieties.

The invention relates to the technical field of plant disease prevention and control and transgenosis, and mainly relates to a rice OsDRM2 gene and application thereof in resisting rice stripe virus diseases.

The invention discloses a stylosanthes guianensis purple acid phytase gene SgPAPhya and a stylosanthes guianensis purple acid phytase gene SgPAPhyb capable of enhancing the aluminum toxicity stress resistance of a plant and application of the stylosanthes guianensis purple acid phytase gene SgPAPhyb, and the aluminum toxicity stress resistance of the plant can be remarkably improved through a transgenic plant in which the SgPAPhya gene and the SgPAPhyb gene are excessively expressed; the SgPAPhya gene and the SgPAPhyb gene play an important role in improving the aluminum toxicity stress resistance of the plants, can be used for improving crops through a transgenic technology and improving the aluminum toxicity stress tolerance of the plants, and have important guiding significance for subsequent cultivation of aluminum toxicity stress resistant crop new germplasm suitable for being planted in acid soil.

The application discloses a Phyllostachys edulis-derived PeDHN2 gene and application of a coded protein thereof, and belongs to the technical field of plant biological breeding. A nucleotide sequence of the PeDHN2 gene is shown as SEQ ID NO. 11; and a sequence of the coded protein of the PeDHN2 gene is shown as SEQ ID NO. 12. The PeDHN2 gene is cloned from the Phyllostachys edulis, and experiments show that the gene is significantly up-regulated under salt stress, drought and abscisic acid (ABA) stress, and after being introduced into rice through a transgenic technology, the germination rate and root growth ability of the rice under salt stress are significantly improved. The application provides a key gene resource and technical support for molecular design breeding of salt-tolerant crops.

The invention discloses a germplasm creation method of hermetia illucens rich in highly unsaturated fatty acids, and belongs to the technical field of insect genetic breeding and aquatic feed raw material development. The fat-1 gene of caenorhabditis elegans is introduced into a hermetia illucens genome through a transgenic technology, a fat-1 gene transfer type hermetia illucens strain is obtained, the HUFA content in the hermetia illucens of the strain is remarkably improved compared with that of a wild type, and particularly, the content of DPA (22: 5n-3), DHA (22: 6n-3) and other essential fatty acids for the human body is remarkably increased. The method can be used for creating excellent germplasm resources of hermetia illucens, and provides an effective technical approach and germplasm resources for developing novel functional aquatic feed and relieving fish meal and fish oil resource pressure.

The present application discloses a wheat heat-resistant protein TaHT2, a coding gene and application thereof. The technical problem is solved by transgenic technology to improve the heat resistance of wheat. The application of the protein, a substance for up-regulating or enhancing or improving the expression of the coding gene of the protein, or a substance for up-regulating or enhancing or improving the activity or content of the protein in improving the heat resistance of wheat is disclosed. The protein is any one of the following: B1) a protein with an amino acid sequence shown in sequence 2, and variants and fusion proteins thereof. The heat resistance of a plant with overexpressed TaHT2 protein coding gene is obviously higher than that of a wild type plant, and the plant can be used for industrial production and plant breeding.

This invention provides an optimized transient expression vector, transfection method, and its applications, relating to the field of transgenic technology. This invention combines UCOE and SINEUP elements to construct a transient expression vector, achieving synergistic effects and further increasing the transient expression level of the target protein while ensuring protein purity and activity. The transient expression vector provided by this invention is highly versatile, applicable to various target proteins and mammalian cells, easy to modify, and has a wide range of applications.

The application discloses a kind of similar bundle-like arabino-galactoside protein coding gene GhFLA2 In the application of regulating cotton seed vigor, the application obtains several overexpression materials, RNA interference materials and gene knockout materials by transgenic technology GhFLA2 It is found that overexpression GhFLA2 Can promote radicle elongation during seed germination and cotyledon unfolding after germination, GhFLA2 After the expression amount of the application is reduced or knocked out, radicle elongation and cotyledon development are inhibited.The application provides a new gene target for cotton seed vigor improvement.

The invention relates to application of a GbPIF3 gene functional structure domain in plant type regulation, which is characterized in that a plant type related gene Gb-PIF3 is obtained by cloning from an apical meristem in a hybrid F1 of sea island cotton-upland cotton, and the functional structure domain GbPIL of the gene is subjected to heterologous expression in arabidopsis thaliana through a transgenic technology. The photoresponse test and seedling property observation on the obtained transgenic material prove that the gene functional structure domain has the function in plant type regulation and control, can be used for transforming the plant type structure of upland cotton and regulating and controlling the flowering time, and has important significance on the improvement of the yield and quality of cotton, especially the genetic modification of close-planting cotton.

The invention relates to the technical field of plant gene transgenosis, in particular to a TaCML15 gene and application of the TaCML15 gene in cultivation of aphid-resistant transgenic wheat. According to the TaCML15 gene, the CDS sequence of the TaCML15 gene is as shown in SEQ ID NO. 1; according to the application of the TaCML15 gene, through overexpression of the TaCML15 gene, the application of improving aphid resistance of wheat or the application of cultivating aphid-resistant transgenic wheat is achieved. In the invention, a TaCML15 overexpression transgenic line shows improvement on insect resistance compared with a wild type, which indicates that the wheat gene TaCML15 participates in insect resistance regulation and control of plants, the discovery of the gene can provide gene resources for crop breeding, and the gene has a relatively wide application prospect.

The application discloses a bamboo plasma membrane H + ATPase, its gene PePMHA9 and application, belongs to the technical field of genetic engineering, and particularly relates to a bamboo plasma membrane H + ATPase, a bamboo plasma membrane H + The amino acid sequence of the bamboo plasma membrane H ATPase is shown as SEQ ID NO. 1. A coding gene PePMHA9 of the bamboo plasma membrane H ATPase, and the nucleotide sequence of the coding gene PePMHA9 is shown as SEQ ID NO. 2. The bamboo plasma membrane H ATPase or the coding gene PePMHA9 is applied to promoting the growth of rice in a high light environment. The bamboo plasma membrane H + ATPase and its coding gene PePMHA9 are successfully separated and identified from bamboo, and are transferred into wild type rice through a transgenic technology to perform function verification, and the obtained transgenic rice has increased leaf stomatal aperture, improved photosynthetic capacity and increased biomass in the high light environment compared with the wild type rice.

The present application discloses a wheat heat-resistant protein TaHT3, a coding gene and application thereof. The technical problem is solved by transgenic technology to improve the heat resistance of wheat. The specific disclosure is the use of the substance for up-regulating or enhancing or improving the expression of the coding gene of the protein or the substance for up-regulating or enhancing or improving the activity or content of the protein in improving the heat resistance of wheat. The protein is the protein with the amino acid sequence shown in sequence 2, and variants and fusion proteins thereof. The heat resistance of the plant with overexpressed TaHT3 protein coding gene is obviously higher than that of the wild type plant, and can be used for industrial production and plant breeding.

Disclosed is a pharmaceutical composition to prevent transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the pharmaceutical composition comprising: genetically modified bacteria; sequences of small peptides; and pharmaceutical excipients, wherein the genetically modified oral bacteria are modified to translate, produce, and emit the sequences of small peptides which neutralize SARS-CoV-2 against COVID-19, wherein transgenic technology is used to modify the genetically modified oral bacteria to add genes in genetically modified oral bacteria that are transcribed to produce small peptides from the sequences of small peptides so added, wherein the sequences of small peptides show extreme binding and neutralization to SARS-CoV-2 but not to host proteins or processes, and wherein the pharmaceutical excipients aid the oral and / or nasal administration of the pharmaceutical composition.

The invention discloses a stripe rust resistance gene YrRC1 derived from roegneria ciliata as well as an encoding protein and application thereof. The stripe rust resistance gene YrRc1 provided by the invention is derived from a roegneria ciliata 2Sc chromosome, the nucleotide sequence of the gene is SEQ ID NO.1, and the amino acid sequence of the gene is SEQ ID NO.2. The invention further discloses a preparation method of the stripe rust resistance gene YrRc1. The invention also protects the application of the YrRC1 in improving the disease resistance of plants. The YrRc1 gene is induced by puccinia striiformis to realize rapid up-regulation expression; a VIGS system is utilized to silence YrRc1 in a disease-resistant material DA2Sc to enhance the plant susceptibility; the YrRc1 gene is introduced into a stripe rust susceptible variety Fielder through a transgenic technology, so that the stripe rust resistance can be remarkably improved. Therefore, the YrRc1 can positively regulate and control the stripe rust resistance and has important significance on wheat disease-resistant breeding.

The invention discloses a CeSUC3a gene capable of promoting early growth and multiple incidence of taro lateral corms and application of the CeSUC3a gene, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the CeSUC3a gene is shown as SEQ ID NO.1. The invention also provides application of the CeSUC3a gene in cultivation of a multi-seed taro variety with early and multiple lateral corms, application of a reagent for up-regulating CeSUC3a gene expression in a host in cultivation of the multi-seed taro variety with early and multiple lateral corms, and the like. According to the invention, the CeSUC3a gene is overexpressed in the multi-seed taro by using a transgenic technology, so that the relative chlorophyll content and net photosynthetic rate of leaves in an overexpressed strain are improved, the lateral corm of the overexpressed strain is promoted to grow early, the seed taro is early grown, the lateral corm is multiple, and the seed taro is multiple grown, so that the early maturing and high yield of the overexpressed strain are promoted, and the yield of the overexpressed strain is improved. Important gene resources are provided for early screening and identification of taro early-maturing high-yield breeding materials and cultivation of early-maturing high-yield taro varieties.