Transgenic method for cultivated silkworm diapause variety

A technology of transgenic and diapause, applied to the transgenic field of silkworm diapause varieties, can solve the problems of very large differences in low-temperature greening, low hatching rate, and relatively large differences in early acid pickling, and achieves a simple and effective method for transgenic operation. Effect

Inactive Publication Date: 2009-08-12
SOUTHWEST UNIVERSITY
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Problems solved by technology

Therefore, the use of polymorphic silkworm varieties as transgenic materials has obvious defects in the following two aspects: ① the economic traits of polymorphic silkworm varieties are poor, and the additional traits created by transgenes cannot reach the standard for direct promotion and application in terms of yield and quality , transgenic strains with polymorphic silkworm eggs as raw materials can only be used as raw materials for breeding, and as the original carrier of a certain related excellent trait, they can be used in the long and arduous traditional breeding
②Pluripotent silkworm varieties do not have a diapause period, and the maintenance of their subculture can only rely on continuous feeding and reproduction, which requires a lot of waste of manpower and material resources to maintain and subculture the obtained transgenic silkworm strains, and also greatly increases The risk of resource loss of obtained transgenic silkworm varieties
Although the above two patents have respectively provided corresponding tr

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Using the silkworm diapause variety "Dazao" as the raw material, the silkworm eggs obtained from normal feeding were incubated in an environment with a constant temperature of 15°C, a relative humidity of 75%, and absolute darkness until hatching. The hatched silkworms were collected every day, placed in a constant temperature of 23°C, relative humidity of 85%, and absolute darkness, and fed with mulberry leaves or artificial feed until the end of three sleeps. The fourth-instar silkworms were quickly transferred to an environment with a constant temperature of 27°C, a relative humidity of 75%, and absolute light, and were fed with mulberry leaves or artificial feed to the upper stack. Transfer the silkworms in the stack to an environment with a constant temperature of 25°C, a relative humidity of 75%, and absolute light. After the pupation is completed, immediately cut off the cocoon shell, and transfer the exposed pupal body to a constant temperature of 26°C and a rela...

Embodiment 2

[0024] Using the silkworm diapause variety "Bi·Bo" as the raw material, the silkworm eggs obtained from normal feeding were accelerated to hatch in an environment with a constant temperature of 15°C, a relative humidity of 75%, and absolute darkness after conventional artificial incubation. The hatched silkworms were collected every day, placed in a constant temperature of 23°C, relative humidity of 85%, and absolute darkness, and fed with mulberry leaves or artificial feed until the end of three sleeps. The fourth-instar silkworms were quickly transferred to an environment with a constant temperature of 27°C, a relative humidity of 75%, and absolute light, and were fed with mulberry leaves or artificial feed to the upper stack. Transfer the silkworms in the stack to an environment with a constant temperature of 25°C, a relative humidity of 75%, and absolute light. After the pupation is completed, immediately cut off the cocoon shell, and transfer the exposed pupal body to a co...

Embodiment 3

[0026] Using the silkworm diapause variety "872" as the raw material, the silkworm eggs obtained from normal feeding were incubated in an environment with a constant temperature of 15°C, a relative humidity of 75%, and absolute darkness until hatching. The hatched silkworms were collected every day, placed in a constant temperature of 23°C, relative humidity of 85%, and absolute darkness, and fed with mulberry leaves or artificial feed until the end of three sleeps. The fourth-instar silkworms were quickly transferred to an environment with a constant temperature of 27°C, a relative humidity of 75%, and absolute light, and were fed with mulberry leaves or artificial feed to the upper stack. Transfer the silkworms in the stack to an environment with a constant temperature of 25°C, a relative humidity of 75%, and absolute light. After the pupation is completed, immediately cut off the cocoon shell, and transfer the exposed pupal body to a constant temperature of 26°C and a relati...

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Abstract

The invention provides a method for making transgenic silkworms of a silkworm diapause variety. The method comprises the steps of utilizing the relationship between the environmental conditions of a parental generation and the diapause property of filial-generation silkworm eggs and utilizing a generation before transgenic operation to control the environmental conditions of the diapause silkworm variety so as to change the diapause property of a present generation of transgenic operation, performing silkworm-egg embryo microinjection on non-diapause silkworm eggs, utilizing fluorescent protein labeling to screen and making the transgenic silkworms. The method has the advantages of ingeniously breaking through the obstacles of transgenic technology for the silkworm diapause variety and effectively overcoming the defects of the prior transgenic method, and can lay a solid technical foundation for the large-scale application of silkworm transgenic technology in functional gene research, bioreactor development and other practical research.

Description

technical field [0001] The invention relates to the fields of biotechnology and bioengineering, in particular to a transgenic method for diapause varieties of silkworm. Background technique [0002] Transgenic technology is an important technology in modern biological research. It can effectively introduce artificially isolated or modified genes into the genome of organisms, and is the most powerful genetic tool for heterologous or ectopic expression. In the post-genome era, transgenic technology has irreplaceable advantages in functional gene research, biological material innovation, new product development, and modern genetic breeding. [0003] The silkworm is an important economic insect and a model organism of Lepidoptera. The silkworm genome frame map (2004) and fine map (2008) have been drawn successively, which marks that the research and understanding of silkworm has officially entered the post-genome era. Using efficient and stable technology to study the function...

Claims

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Application Information

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IPC IPC(8): C12N15/85A01K67/04
Inventor 马三垣夏庆友徐汉福赵爱春向仲怀
Owner SOUTHWEST UNIVERSITY
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