197 results about "Fluorescent protein" patented technology

The invention discloses an oil palm U6 promoter gene and application thereof, and belongs to the technical field of biology. The nucleotide sequence of the promoter gene is shown as SEQ ID NO.1. The oil palm RNA polymerase III type promoter gene, namely the oil palm endogenous U6 promoter gene EgU6, is obtained by cloning in an oil palm genome for the first time, and the promoter gene has high transcriptional activity and can drive downstream fluorescent protein mNeonGreen expression. The candidate oil palm endogenous U6 promoter gene can be provided for subsequently establishing a high-efficiency oil palm gene editing technology system based on a CRISPR / cas9 (Clustered Regularly Interspaced Short Palindromic Repeats / Cas9) system.

The invention discloses a construction method and application of a probiotic engineering bacterium EcNpAm targeting animal intestinal tracts and improving inflammation. According to the present invention, the heterologous expression of the immunomodulatory protein Amuc1100 is successfully achieved through the gene engineering technology by using the Escherichia coli Niss1917 (EcN) as the host and using the anaerobic inducible plasmid pnirBMisL as the expression vector, and the novel engineering bacterium EcNpAm suitable for the animal intestinal anaerobic environment is constructed; furthermore, the expression efficiency of the target protein is represented by fusion expression of green fluorescent protein (GFP). The engineering bacterium is applied to prevention and treatment of animal intestinal inflammation, and successfully realizes targeted delivery of therapeutic protein to intestinal tracts of mice. Experiments show that EcNpAm can effectively relieve typical symptoms such as weight loss, colon shortening, pathological injury, colitis and the like of mice induced by dextran sodium sulfate (DSS) and protect integrity and functions of an intestinal barrier structure.

The invention discloses a rapid degreasing method for a biological tissue sample, which comprises the following steps: sequentially carrying out fixing, tissue dehydration, azeotropic degreasing, tissue rehydration and other treatments on the sample, and the degreasing solution is composed of alcohols and hydrocarbons according to a mass ratio of 1: 1-1: 2. According to the rapid degreasing method provided by the invention, the lipid in the tissue is efficiently extracted through mixed azeotropy of the alcohol and hydrocarbon organic reagents, so that the tissue is transparent and transparent; the problem of poor degreasing in tissues is solved by utilizing the high permeability characteristic of the micromolecular organic reagent; the dissolving capacity to phospholipid and cholesterol is improved by mixing organic reagents; through azeotropy, the degreasing efficiency is improved, the degreasing time is shortened, the internal permeability effect of the tissue is improved, and internal and external uniform permeability of the tissue is realized; by adjusting the components of the organic reagent, boiling rapid degreasing at low temperature is realized, and the retention of a fluorescence signal is improved; the tissue treated by the method is transparent and small in deformation, a fluorescent protein signal can be maintained, and the immunofluorescence staining effect can be improved.

Protease activity can be assessed using a microfluidic system that comprises a microfluidic device and solid support, wherein the solid support comprises a protease fusion protein bound to a first surface of the solid support; wherein the protease fusion protein comprises a protease domain, a protease activation sequence, and a fluorescent protein tag, wherein the fluorescent protein tag is bound to the protease domain and to the first surface of the solid support. Protease activity can be assessed in a microfluidic system by reacting an activating protease with a protease fusion protein; wherein the protease fusion protein comprises a protease domain and a protease activation sequence; wherein the activating protease cleaves the protease activation sequence, thereby activating the protease domain in the protease fusion protein to produce an activated protease fusion protein; reacting the activated protease fusion protein with a detectable substrate, thereby generating a detectable signal; and detecting the detectable signal, thereby detecting protease activity in the microfluidic system.

The invention provides a recombinant herpes simplex virus and a herpes simplex virus modification method. The herpes simplex virus modification method comprises the step of replacing an ICP4 protein coding gene in a herpes simplex virus genome containing an infected cell protein 4 (ICP4) gene with an hTERTp-fluorescent protein expression cassette. The expression cassette comprises an hTERTp promoter and a fluorescent protein coding sequence controlled by the hTERTp promoter, and the transcription direction is opposite to that of an ICP4 promoter in a genome. The hTERTp-fluorescent protein expression cassette in the recombinant virus obtained by the method disclosed by the invention can be normally expressed in response to telomerase activity. Therefore, the recombinant virus is capable of expressing a fluorescent protein, such as mBaoJin, in a cell having human telomerase activity. Cells infected by the virus can be identified through fluorescence signals, and the higher the telomerase activity is, the stronger the fluorescence intensity is. The virus has wide application value in research of tumor action mechanisms and stem cell action mechanisms, health assessment, screening of tumor drugs, research and development of diagnostic reagents and establishment of animal models.

This invention provides a method for constructing a fluorescently traced mouse model and its application in the sorting of Mcpt4-positive and negative mast cells. First, the Cre gene is knocked into the Mcpt4 gene sequence of C57BL / 6 mice using CRISPR / Cas9 technology. Then, through animal hybridization and Cre-loxP recombination technology, a fluorescently traced mouse model of Mcpt4-positive mast cells is obtained. The fluorescently traced mouse model possesses a dual-fluorescent reporter gene system consisting of the red fluorescent protein tdTomato and the green fluorescent protein ZsGreen. Mcpt4-positive mast cells exhibit red fluorescence, while other Mcpt4-negative cells exhibit green fluorescence. Using this fluorescently traced mouse model, Mcpt4-positive and negative mast cells can be rapidly, accurately, and effectively sorted, providing a reliable technical basis for exploring the roles and mechanisms of Mcpt4-positive and negative mast cells in immune responses.

The application provides a construction of a fluorescent protein labeled microtubulin and a microtubule binding protein universal bivalent carrier, and the process is as follows: a first stage is to construct a GFP-alpha tubulin carrier, and a second stage is to construct a GFP-alpha tubulin-mCherry universal bivalent co-expression carrier; in the second stage, 35S, mCherry and NOS sequences are inserted into a KpnI enzyme cutting site of a multiple cloning site of the GFP-alpha tubulin carrier constructed in the first stage, and a single nucleic acid enzyme cutting site is inserted into the 5' end and the 3' end of the mCherry sequence, respectively, and the single nucleic acid enzyme cutting site is XbaI, KpnI / Acc65I and AscI sequences, respectively, so as to obtain the universal bivalent carrier. The universal bivalent carrier provided by the application can express two target genes simultaneously, has the fluorescent signals of GFP and mCherry, can quickly and accurately identify a transgenic plant, is convenient for positive seedling screening, can be used for a tobacco transient expression experiment, can shorten an experimental period, and saves time and effort.

The invention discloses a monoclonal cell strain screening method based on fluorescence intensity, which comprises the following steps: determining a cell line, and stably transfecting fluorescent protein to cells of the cell line; a microwell plate is selected, a datum hole is set, the cells stably transfected with the fluorescent protein are separated and put into the microwell plate, a plurality of cells are put into the datum hole, and only one cell is put into the other holes; screening out single-cell pores with only one cell from the rest of pores except the reference pore; screening out clone group holes capable of growing clone groups; respectively digesting cells in the cloning group pores and then transferring the cells into new pores for culturing; screening out fluorescent screening holes; splitting proliferation holes are screened out; and determining the cells in the division proliferation hole as the screened monoclonal cell strain. According to the monoclonal cell strain screening method provided by the invention, the monoclonal cell strain which is high in fluorescence intensity and can proliferate at a normal speed can be screened, compared with fluorescence microscope observation and imaging, the method is convenient and rapid, and errors possibly generated by comparing the fluorescence intensity with human eyes are reduced.

The present invention relates to a method for sanitising, disinfecting or sterilising microbially contaminated water-containing fluids, comprising treating the microbially contaminated water-containing fluid with an oxidising agent, wherein a fluorescence measurement is carried out on the water-containing fluid before, after and / or during treatment of the microbially contaminated water-containing fluid with the oxidising agent. The fluorescence measurement comprises irradiation with an excitation wavelength characteristic of a fluorescent protein and / or peptide building block and measurement of the corresponding fluorescence signal, and the method is characterised in that treatment with the oxidising agent proceeds in such a way that addition of the oxidising agent is controlled as a function of the fluorescence signal. The invention further relates to a corresponding device for sanitising, disinfecting or sterilising microbially contaminated water-containing fluid.

The invention relates to the field of medicine. In particular, the present invention relates to a protein-based biosensor comprising a substrate binding protein that binds to an MRI contrast agent by a chelating agent. More specifically, the present invention relates to a glutamic acid biosensor comprising a glutamic acid binding protein bound to an MRI contrast agent, characterized in that the glutamic acid binding protein comprises SEQ ID NO: 10, preferably SEQ ID NO: 5, and a sequence encoding a fluorescent protein.

The invention belongs to the technical field of GPCR drug research and development, and discloses a ratio type cAMP fluorescent probe RaCamp and a construction method of a transgenic mouse, the probe RaCamp is formed by series fusion of G-Flamp2 fluorescent protein and mCherry fluorescent protein through a flexible linker, and the preparation method of the probe RaCamp comprises the following steps: S1, obtaining an mCherry fragment; s2, a G-Flamp2 linear carrier is obtained; s3, homologous recombination; s4, converting and screening; the construction method of the transgenic mouse comprises the following steps: T1, designing a carrier; t2, microinjection of fertilized eggs; t3, embryo transplantation and reproduction; the invention has the beneficial effects that mCherry is added as an internal reference protein on the premise of keeping the sensitivity of the original G-Flamp2 probe, and the selected connecting peptide (GGGGS) 2 enables the expression quantity of the two proteins in cells to be accurately controlled to be 1: 1, so that the problem of background interference in cell observation of in-vivo imaging is well solved. And the sequence is inserted into a Rosa26-LSL box, so that the construction of a Rosa26-LSL-RaCamp mouse is realized.

This application discloses an embedded whole-cell biosensor, its fabrication method, and its application in machine learning-assisted mercury ion detection, belonging to the field of sensor materials and detection technology. This sensor encapsulates *E. coli* expressing green fluorescent protein (GFP) in a polyacrylamide / hyaluronic acid (PAAM / HA) hydrogel matrix, enabling highly selective and sensitive detection of mercury ions. It effectively eliminates interference from competing metal ions and can be completely regenerated through EDTA-2Na treatment, allowing for repeated use without performance loss. It maintains a stable fluorescence response for up to three weeks, possesses excellent biocompatibility and biodegradability, minimizing the risk of secondary contamination, and facilitates rapid on-site analysis.

The invention discloses a method for bifluorescence quantitative detection of epithelial cells of a lacrimal gland organ and application. The method comprises the following steps: providing a mouse lacrimal gland organ; a recombinant adeno-associated virus combination is added to the organoid, the combination comprising: a first AAV vector comprising a promoter specifically driving the expression of alveolar epithelial cells and a first fluorescent protein coding sequence; the second AAV vector contains a promoter for specifically driving expression of myoepithelial cells and a second fluorescent protein coding sequence; according to the method, the two cells can be visually distinguished, and by calculating the fluorescence area or the number of the cells, the alveolar / muscular epithelial cell proportion is established as a damage index, so that high-throughput drug screening is realized.

The invention discloses a synchronous imaging method for a multicellular structure in a multicellular type. The synchronous imaging method comprises the following steps: S1, marking the cellular structure in the multicellular type by using fluorescent protein; s2, forming a spectrum-spatial feature joint coding set according to the spectrum and spatial features; s3, performing spectral unmixing on the spectral image to obtain an abundance image of each fluorescent component; s4, performing cell segmentation and cell structure positioning, and extracting spatial features; and S5, carrying out joint decoding on the spectral features and the spatial features to distinguish different cell types, and carrying out synchronous imaging on the multi-cell structure. The method has the beneficial effects that different cell types are marked by using corresponding fluorescent proteins, and a spectrum-space coupling coding strategy is formed in combination with spatial position characteristics, so that simultaneous observation and dynamic analysis of different cell types and a plurality of internal structures in the same imaging view field are realized; and the limitation that traditional spectral imaging can only work in a single cell type is broken through.

Fluorescent proteins and split-fluorescent proteins (SFPs) including split-green fluorescent proteins from Corynactis californica are provided. Nucleic acid molecules encoding the fluorescent proteins and SFPs, vectors, and kits are also provided. Methods of using the fluorescent proteins and SFPs, such as detecting a protein of interest or protein-protein interactions are also provided.

The invention relates to the fields of molecular biology and virology, and provides a method for preparing a specific MHC haplotype-derived cell-binding virus. The method comprises the following steps: firstly, obtaining chick embryos of different haplotypes, separating CEF cells, transiently expressing exogenous fluorescent protein through electrotransfection, then carrying out virus inoculation, carrying out flow sorting to obtain a fluorescent positive cell population carrying MDV, and carrying out quantitative analysis on the cell population to obtain the specific MHC haplotype chicken Marek's Disease Virus (MDV), thereby obtaining the specific MHC haplotype chicken Marek's Disease Virus Marek's Disease Virus Marek's Disease Virus Marek's Disease Virus. And carrying out in-vitro propagation to obtain the purified MDV with a specific haplotype source. The purified virus can provide a test material for related research of haplotype on virus infection, and can be widely applied to in-vitro purification of different cell binding viruses and related research of specific MHC molecule presenting virus peptides. The method disclosed by the invention is beneficial to carrying out related research aiming at the host immune response of the virus and analyzing the hereditary basis of haplotype to MDV differential resistance, and is expected to be applied to preparation of vaccine virus.

The invention belongs to the technical field of transgenic and biomedical research tools, and particularly relates to a method for constructing a transgenic fish system based on a Tol2 transposon system, which comprises the following steps: constructing a myo6b-lhfpl5b-DsRed plasmid by using myo6b as a promoter, lhfpl5b as a target gene and DsRed as a red fluorescent protein tag through a microinjection method, and constructing a Tol2 transposon system-based transgenic fish system. Co-injecting the plasmid containing the Tol2 transposase mRNA and the Tol2 transposase mRNA into a fertilized egg of an AB zebra fish strain, and screening generation by generation to obtain a transgenic fish strain with stable inheritance MET channel gene lhfpl5b marked hair cells. The spatio-temporal dynamic visualization of the living whole fish to the lhfpl5b is realized, the deafness-causing molecular pathway is clarified, and an in-vivo platform is provided for drug screening and intervention strategies.

Provided are nucleic acids encoding kinase-modulated bioluminescent indicator (KiMBI) polypeptides. In certain embodiments, a nuclei acid of the present disclosure encodes a KiMBI polypeptide comprising a first bioluminescent enzyme fragment, a phospho-binding domain, a 5 second bioluminescent enzyme fragment capable of forming an active bioluminescent enzyme with the first fragment via enzyme fragment complementation, and a kinase substrate bound by the phospho-binding domain when phosphorylated. KiMBI polypeptide may be fused to one or more fluorescent proteins, e.g., which exhibit resonance energy transfer (RET). Also provided are KiMBI polypeptides encoded by the nucleic acids of the present disclosure. Cells that express 10 a KiMBI polypeptide are also provided, as are non-human animals comprising such cells. Also provided are methods of assessing activity of a kinase of interest in a non-huma animal, and methods of assessing a test agent for the ability to inhibit a kinase of interest in a non-human animal.

The invention relates to a method for screening an anti-Alzheimer's disease compound based on a high content imaging technology, and the method comprises the following steps: S1, carrying out splitting decomposition and centrifugation on nematodes to remove a supernatant, and then carrying out resuspension culture to obtain a synchronized nematode solution in an L1 period, the nematodes carrying a human-derived beta amyloid protein gene connected in series with a fluorescent protein gene; s2, adding a synchronized nematode solution in the L1 period to an NGM solid culture medium paved with a candidate anti-Alzheimer's disease compound, culturing to the L4 period, collecting nematodes, adding an OP50 bacterial solution, and inducing human beta amyloid protein gene expression; s3, collecting nematodes for inducing human beta amyloid protein gene expression, and performing fluorescence image shooting by using a high content imaging technology; and S4, analyzing the fluorescence intensity in the picture. Compared with the prior art, the high-content imaging technology is adopted, the defects that a traditional A beta fluorescence detection method in a CL2331 nematode model is low in efficiency and long in consumed time are overcome, the detection sensitivity is improved by 27.63%, and the efficiency is improved by 40 times.

The invention belongs to the technical field of genetic engineering, and particularly relates to a universal bacterial artificial chromosome recombinant virus transfer vector and a construction method thereof, the transfer vector comprises a BAC amplification vector with pUC19-loxp-gpt-IRES-EGFP as a basic skeleton and pUC19-BAC as a functional sequence; according to the universal bacterial artificial chromosome recombinant virus transfer vector and the construction method thereof, a drug screening target gene reading frame and an EGFP fluorescent protein gene reading frame are connected through an IRES double-expression connection sequence, and double screening can be carried out; in the BAC construction process, screening genes and functional sequences are respectively constructed to a high-copy T vector, so that sufficient plasmid DNA can be conveniently copied and prepared. And through a proper restriction enzyme cutting site, introduction of various homologous arms is facilitated, the alignment is wide, simultaneous operation of various genomic sequences is facilitated, and the research efficiency is improved.

The invention discloses a genetically engineered bacterium for producing D-pantothenic acid based on a corynebacterium glutamicum CPSPC system as well as a construction method and application of the genetically engineered bacterium. Wild type C.glutamicum ATCC 13032 is taken as a chassis strain, and a functional combination of a glycine ribosome switch BsGR from bacillus subtilis and a promoter Ptuf / Ph36 is verified through fluorescent protein mCherry; a GCS system of escherichia coli and bacillus subtilis is introduced to the strain DPAg-15, genes related to synthesis of 5, 10-dimethyltetrahydrofolic acid are tested, it is found that serA delta197GTG / ATG significantly increases the yield of D-pantothenic acid, the yield of D-pantothenic acid of the constructed strain DPAj-2-tuf is increased to 23.06 g / L (63 h), the yield is 0.37 g / L / h, and the conversion rate is 0.17 g / g; the engineering strain DPAj-2-tuf is obtained by dynamically regulating and controlling a D-pantothenic acid synthesis path through a CPSPC system and combining plasmid overexpression serA delta197GTG / ATG, the yield of the engineering strain DPAj-2-tuf is increased by 2.1 times compared with that of a traditional method, and an efficient technical scheme is provided for producing D-pantothenic acid through a microbial fermentation method.

The invention relates to the technical field of biology, in particular to a red fluorescent protein mCrimsonX as well as a construction method and application thereof. The invention relates to a red fluorescent protein mCrimson X. The mutant amino acids of the red fluorescent protein mCrimson X relative to Crimson comprise N8E, R10P, M15L, S18T, I70V, Y72H, A74K, D75G, F79Y, T94M, Y96F, E111Q, E114V, V116I, V119A, M147I, I160M, N173S, T177E, H193Y and V195I. The invention further discloses a preparation method of the red fluorescent protein mCrimson X. The preparation method has the advantages that mCrimsonX is high-performance long-wavelength fluorescent protein in a visible region; special proteins which cannot be accurately marked by other red fluorescent proteins can be marked; the monomer property is very good, and the extinction coefficient and the quantum yield are relatively high. The method can be used for constructing cell function probes and is wide in application range.

The invention provides a detection method for reducing the off-target risk of a targeting drug, and relates to the technical field of immunodetection. The detection method comprises the following steps: screening the optimum working concentration of a test substance, screening the human plasma membrane protein having a binding risk with the test substance, and screening out a target protein having a specific binding relationship with the test substance; according to the detection method provided by the invention, the expression of the fluorescent protein can indicate the expression condition of the human plasma membrane protein, positive control, quality control control and negative control are set, and the target protein having a specific binding relationship with a test substance is obtained through chemiluminescence detection and flow cytometry screening, so that the risk of a false negative result is reduced, and the detection accuracy is improved. And the accuracy of predicting the off-target risk of the targeted drug is improved.

The invention discloses a fluorescent probe for detecting G-quadruplex, and belongs to the technical field of biomedical detection. The fluorescent probe comprises a fusion protein, the fusion protein comprises a peptide fragment A, the peptide fragment A can be specifically combined with a G-quadruplex, and the amino acid sequence of the peptide fragment A is as shown in SEQ ID NO.1; and the cyclic rearrangement fluorescent protein is operably connected with the peptide fragment A. The fluorescent probe not only can specifically recognize in an in-vitro sample and correspond to various G4 conformations, but also can realize high-resolution imaging in living cells, promotes basic research of G4 functions, and opens up a new way for transforming medicine and clinical application.

The invention discloses an exocytosis protein subcellular localization method for mannitol-induced plasm-wall separation, and relates to the technical field of cytobiology, the method comprises the following specific steps: constructing a fusion expression vector: connecting a plant target exocytosis protein gene with a fluorescent protein gene through a gene cloning technology to obtain a fusion expression vector; inserting into a plant expression vector to obtain a recombinant expression vector containing the target extracellular secretory protein-fluorescent protein fusion gene; performing bacterial colony PCR identification and sequencing verification on the recombinant expression vector; by adopting an agrobacterium tumefaciens-mediated plant leaf instantaneous transformation technology, the fusion gene is rapidly expressed in plant cells, subsequent experiments can be carried out after culture is carried out for 2-3 days, the experiment period is greatly shortened, meanwhile, leaf cells are induced to be subjected to plasmon-wall separation through a gradient mannitol solution, positive cell screening and identification are not needed, and the application prospect is wide. And positioning can be realized by directly observing fluorescence signal distribution by using a laser confocal microscope.

The invention belongs to the technical field of animal reproductive biology and cell engineering, and particularly relates to a fluorescence labeling expression system for visualizing an oocyte nucleus maturation process and application of the fluorescence labeling expression system. The fluorescence labeling expression system comprises an eukaryotic expression vector pVenus-LMNB1; the eukaryotic expression vector pVenus-LMNB1 comprises a nucleotide sequence for coding the fusion protein Venus-LMNB1. According to the invention, a nuclear fiber layer protein stably expressed on a nuclear membrane is selected as a research object, a eukaryotic expression vector pVenus-LMNB1 is constructed by using a molecular cloning technology, an LMNB1 gene containing a green fluorescent label Venus is overexpressed in a porcine oocyte by using a microinjection technology, fluorescent protein labeling of a structural protein on the nuclear membrane is utilized, and the expression vector pVenus-LMNB1 is constructed. The expression positioning condition of the LMNB1 in the maturation process of the porcine oocytes is observed, and a powerful tool is provided for development of in-vitro culture related researches of the porcine oocytes.

The application provides a method for detecting secretion expression of a target protein, which comprises the following steps: fusing the target protein with a fluorescence-enhanced nanobody to obtain a fusion protein; adding the obtained fusion protein into a system containing a fluorescent protein and a fluorescence-inhibited nanobody to perform a reaction; and measuring the fluorescence intensity of the system after the reaction is completed, and calculating the secretion expression amount of the target protein according to the change amount of the fluorescence intensity. In the application, the GBP1 is fused with the target protein, and the expression interference of the GBP1 on the target protein is reduced by adding a connecting peptide, so that the secretion expression amount of the target protein is determined. The method of the application can detect a wide concentration range of the target protein, has a high signal response multiple, and can be applied to a shake flask, a well plate and a droplet microfluidic screening system.

The present invention relates to a method for hygienizing, disinfecting or sterilizing microbiologically contaminated aqueous fluids, comprising treating the microbiologically contaminated aqueous fluid with an oxidizing agent, wherein a fluorescence measurement is performed on the aqueous fluid before, after and / or during the treatment of the microbiologically contaminated aqueous fluid with the oxidizing agent, which comprises irradiation with an excitation wavelength characteristic of a fluorescent protein and / or peptide building block and measuring the corresponding fluorescence signal, characterized in that the treatment with the oxidizing agent is carried out in such a way that the addition of the oxidizing agent is controlled depending on the fluorescence signal.The present invention further relates to a corresponding device for the hygienization, disinfection or sterilization of microbiologically contaminated aqueous fluid.

The invention discloses an Escherichia coli strain capable of stably expressing near-infrared fluorescent protein as well as a construction method and application of the Escherichia coli strain, and belongs to the technical field of gene engineering. According to the bacterial strain, escherichia coli probiotics Nissle 1917 serve as a chassis bacterial strain, at least two miRFP713 gene expression cassettes are integrated on a chromosome through a CRISPR-Cas mediated homologous recombination technology, and each expression cassette comprises a constitutive promoter and a miRFP713 gene optimized by a codon; meanwhile, the biliverdin synthetase gene is integrated to realize cofactor self-supply. The chromosome integrative Escherichia coli strain constructed by the invention realizes stable expression without exogenous induction, the fluorescence signal retention rate of 20 generations of passage is greater than 98%, and the fluorescence intensity is improved by 94.8 times, and is suitable for multiple application scenes such as long-term living body tracking, tumor targeted tracking and the like.

The invention belongs to the field of synthetic biology and metabolic engineering, and discloses a screening method and application of a microbial strain with high yield of ergothioneine, a promoter (such as PECL1, PBSC5, PEDC2 and the like) responding to ergothioneine is screened through transcriptomics analysis based on saccharomyces cerevisiae, and the promoter can present high sensitivity and linear response under 0-1000 mg / L of ergothioneine. The strain is used for driving fluorescent protein gene expression, a fluorescence report strain is constructed, the strain can rapidly and sensitively detect the concentration of ergothioneine in fermentation liquor by virtue of fluorescence signal change, and high-yield strains are efficiently screened. The method overcomes the problems of low flux, long period, low sensitivity and the like of traditional screening, and the fluorescence signal intensity is in significant linear correlation with the ergothioneine concentration. And the perforated plate and the microplate reader are used for automatic high-throughput screening, so that the efficiency is improved, the resource consumption is reduced, effective support is provided for production optimization of ergothioneine, and the application prospect is wide.