782 results about "Phosphatase" patented technology

The invention belongs to the technical field of biological medicine, and discloses a probiotic agent capable of improving the physiological activity function of vitamin D and maintaining bone health and application of the probiotic agent. The invention provides a probiotic agent composed of bifidobacterium adolescentis and bifidobacterium longum, the probiotic agent can significantly improve the bone mineral density, the bone trabecula thickness, the bone trabecula number, the bone trabecula connectivity and the bone volume fraction of an osteoporosis mouse, and meanwhile, the bone trabecula separation degree of the osteoporosis-removed mouse is significantly reduced; the level of vitamin D metabolites such as 25-hydroxy vitamin D and 1, 25-dihydroxy vitamin D in mouse serum is improved; the content of serum calcium and alkaline phosphatase in serum of mice is reduced, and the level of osteocalcin and type I procollagen amino terminal peptide in the serum is improved. The probiotic agent can relieve bone calcium loss, maintain bone health, repair bone injury, increase osteoblasts and reduce the osteoporosis degree, and can be used for preparing drugs for preventing and / or treating osteoporosis or preparing health care products or food beneficial to improving bone mineral density.

The invention provides a plant lactobacillus TH13, and the preservation number of the plant lactobacillus TH13 is CGMCC (China General Microbiological Culture Collection Center) NO.32112. The plant lactobacillus TH13 provided by the invention can be used for fermenting folium cortex eucommiae and enzymatically hydrolyzed bone meal, and the fermentation product of the plant lactobacillus TH13 has high content of chlorogenic acid, proline, glycine and lysine. The fermentation product can significantly improve the content of alkaline phosphatase (ALP) and osteocalcin (OCN) in mouse serum and reduce the content of tartrate-resistant acid phosphatase (TRACP). Therefore, the fermentation product can significantly improve the bone mineral density of the femur, increase the number of bone trabecula and significantly reduce the separation degree of the bone trabecula, thereby promoting bone formation of a mouse with senile osteoporosis, inhibiting bone resorption and improving senile bone loss and bone microstructure deterioration. The preparation method of the probiotic preparation containing the plant lactobacillus and the probiotic fermentation product is simple, and large-scale production can be realized.

The invention relates to a method for improving chemiluminescence immunoassay sensitivity of magnetic particles, a detection kit and application thereof, firstly, magnetic beads are coupled with an antibody, then magnetic beads are sealed to obtain a magnetic bead coated antibody, sealing comprises pre-sealing and second-step sealing, glycine and ethanolamine are added in the pre-sealing process, and in the second-step sealing process, glycine and ethanolamine are added in the second-step sealing process. Casein hydrolysate and dodecyl dimethyl amine ethyl lactone are added; then coupling the antibody with alkaline phosphatase or streptavidin to obtain an alkaline phosphatase labeled antibody or a streptavidin labeled antibody; the magnetic bead coated antibody, the alkaline phosphatase labeled antibody or the streptavidin labeled antibody is used for sample detection. The method is high in sensitivity and wide in detection range, is adaptive to a common immunoassay instrument, does not need expensive equipment, and is also simple to operate; in addition, the AMH concentration with extremely low concentration can be accurately detected.

The invention discloses a D-psicose 6-phosphate phosphatase mutant and application of the D-psicose 6-phosphate phosphatase mutant in preparation of psicose, and belongs to the technical field of enzyme engineering. According to the invention, site-directed mutagenesis is carried out on an amino acid sequence of D-psicose 6-phosphate phosphatase A6PP, and a combined mutant I21V / Y73F / R115M / T180V / L203I / Q193E is constructed, so that the substrate specificity, the thermal stability and the catalytic activity of the combined mutant are all remarkably improved. The invention further provides application of the combined mutant in preparation of psicose through multi-enzyme cascade catalysis, 150 g / L of maltodextrin can be converted into 97.7 g / L of D-psicose, the conversion rate reaches 65.1%, and the application prospect of the combined mutant in the field of food is widened.

The invention provides an organophosphorus mineralization composite flora with wide pH (potential of hydrogen) adaptability and application of the organophosphorus mineralization composite flora. The organophosphorus mineralization composite flora comprises subterranean bacillus subterraneus, stenotrophomonas sp and Cupriavidus sp. The organophosphorus mineralization composite flora has the advantages that the organophosphorus mineralization composite flora has wide pH adaptability, and the organophosphorus mineralization composite flora comprises the subterranean bacillus subterraneus, the stenotrophomonas sp. And the Cupriavidus sp. The organophosphorus mineralization composite flora provided by the invention can be widely used in acid-base environments; wherein the capacity of the phosphatase secreted by the three bacteria is not interfered by the external phosphorus concentration, and the phosphatase is constitutive phosphatase, so that a large amount of phosphatase can be secreted no matter which phosphorus concentration is high or low. The organophosphorus mineralization composite flora remarkably promotes mineralization of organophosphorus and release of phosphorus in the organic fertilizer, improves the nutrient efficiency of the organic fertilizer, and can play a role in mineralizing organophosphorus in both acid soil and alkaline soil.

The invention discloses a comprehensive prevention and control method for tobacco root knot nematode disease containing a biological agent. The method comprises the steps of crop rotation of tobacco and buckwheat / quinoa, host resistance induction of the microbial agent in the seedling raising period, tobacco planting soil quality improvement in the transplanting period and the like. The agronomic characters of tobacco seedlings are effectively improved, and the physical characters of soil are improved; the chemical properties of soil pH, alkali-hydrolyzable nitrogen, available phosphorus, quick-acting potassium, exchangeable calcium, exchangeable magnesium and the like are improved; the activity of soil urease, sucrase, acid phosphatase, catalase and peroxidase is improved; the content of water-soluble phenols, compound phenols and total phenols in soil is reduced; the disease index of the tobacco root knot nematode disease can be remarkably reduced, the survival rate of tobacco is increased, the application amount of chemical agents is reduced, and the healthy and sustainable development of the tobacco industry is promoted.

The invention discloses bifidobacterium longum subsp.infantis capable of promoting bone growth and a metagen thereof, and belongs to the technical field of biology. The bifidobacterium longum subsp. Infantis CCFM1445 provided by the invention can promote the increase of bone volume fraction, femoral bone trabecula thickness and far-end growth plate thickness of mammals in a growth period, reduce the levels of serum bone resorption markers I-type collagen amino-terminal peptide and tartrate-resistant acid phosphatase, improve the levels of serum bone alkaline phosphatase and parathyroid hormone, and improve the clinical application value of the bifidobacterium longum subsp. Infantis CCFM1445. And the relative abundance of the other bacteria in the intestinal flora and the levels of creatine and indole-3-lactic acid are increased. The thermally inactivated bifidobacterium longum subsp. Infantis CCFM1445 can promote the increase of the volume fraction of femoral bones and the number of bone trabecula of malnourished mice and improve the N-terminal propeptide level of serum I-type procollagen. Therefore, the medicine or health care product prepared from the bifidobacterium longum subsp. Infantis CCFM1445 can be used for assisting in regulating bone metabolism and bone mass, and has a wide market prospect.

The invention belongs to the technical field of bioengineering, and particularly relates to a polyphosphate kinase mutant and application thereof in synthesis of beta-nicotinamide mononucleotide, and the polyphosphate kinase mutant is obtained by single-point or multi-point mutation of 99th, 186th, 190th and 204th amino acids of polyphosphatase with an amino acid sequence shown as SEQ ID NO.1. The invention also relates to a preparation method of the polyphosphate kinase mutant, and the application of the polyphosphate kinase mutant in synthesis of beta-nicotinamide mononucleotide in synthesis of beta-nicotinamide mononucleotide in synthesis of beta-nicotinamide mononucleotide in synthesis of beta-nicotinamide mononucleotide. Polyphosphokinase derived from Lampropedia Hyalina DSM 16112 is subjected to molecular modification to obtain multiple mutants, the enzyme activity of the mutants is improved by 1.5-14 times compared with that of wild type polyphosphokinase, the preference of the mutants to AMP is improved by 1.08-4.88 times compared with that of the wild type polyphosphokinase, and an ATP regeneration system constructed by the mutants can remarkably reduce the use amount of enzyme and reduce the fermentation capacity and cost; the reaction time can be greatly shortened, the influence of AMP on the enzyme activity is reduced, the requirements of large-scale industrial production of NMN prepared by adopting a biological enzyme method can be met, and the method has a wide application prospect.

The method for preparing D-mannose through catalysis can effectively reduce the amount of by-products glucose and fructose, and greatly improves the conversion rate of D-mannose. Specifically, engineering bacteria for expressing isoamylase genes and engineering bacteria for expressing alpha-glucan phosphorylase genes, glucophosphate mutase genes, difunctional enzyme glucophosphate isomerase / mannose 6-phosphate isomerase genes and mannose 6-phosphate phosphatase genes are utilized, starch or starch derivatives are used as substrates, and the starch or starch derivatives are used as substrates. A phosphate buffer solution and Mg < 2 + > are added, a preliminary catalysis system is constructed, after the reaction is completed, an incompletely-reacted substrate and maltodisaccharide exist in the reaction system, the incompletely-reacted substrate and maltodisaccharide can be hydrolyzed into glucose by glucoamylase, and the polyphosphoglucokinase can be used for catalyzing the reaction of the polyphosphoglucokinase under the assistance of sodium hexametaphosphate. All glucose is converted into phosphorylated glucose, then D-mannose is generated, and by-products in a system are removed while the yield is increased.

The invention provides a method for producing borneol, the method comprising culturing the transgenic microorganism in a culture medium, and the microorganism comprises or expresses borneol diphosphate synthetase and phosphatase.

The invention belongs to the technical field of preparation of polypeptides, and particularly relates to a self-assembled polypeptide capable of responding to alkaline phosphatase and degrading Neuropilin-1 as well as a preparation method and application of the self-assembled polypeptide. The self-assembled polypeptide (formula I) provided by the invention has a self-assembly starting unit 'GNNQQNY (SEQ ID NO: 1)', can be self-assembled in vitro, and can form nanofibers after responding to alkaline phosphatase, so that not only can the critical self-assembly concentration of the polypeptide be reduced, but also the binding capacity with target protein can be improved. The material can degrade NRP1 on the surfaces of tumor cells and Treg cells, and has huge potential in promoting immunotherapy of tumors.

The invention relates to the field of biomedicine, particularly provides a copper-based nano-enzyme as well as a preparation method and application thereof, and aims to solve the problems that in the prior art, clinical treatment on primary sclerosing cholangitis (PSC) is difficult in diagnosis and lacks of effective treatment drugs. The copper-based nano-enzyme comprises a nano-enzyme carrier and a copper-based nano-enzyme, wherein the nano-enzyme carrier is a two-dimensional nanosheet formed by copper ions, gallic acid and ursodesoxycholic acid through coordinate bonds; the probe molecule is a cyanine dye molecule connected to the surface of the nano-enzyme carrier through a covalent bond; wherein the fluorescence intensity of the copper-based nano enzyme is enhanced after the action of the alkaline phosphatase. The three functions of alkaline phosphatase responsive diagnosis, nano-enzyme catalytic treatment and ursodesoxycholic acid hepatic targeting are innovatively and synergistically integrated into one nano platform, the treatment function can be executed while specific imaging diagnosis is performed on diseases, and a new strategy is provided for diagnosis and treatment of diseases such as PSC.

The invention belongs to the technical field of preparation and application of nano materials, and relates to a preparation method and application of amino-functionalized cerium oxide nano enzyme, and the preparation method comprises the following steps: adding 3-aminophenylboronic acid into a cerous nitrate hexahydrate aqueous solution to obtain a mixed solution; dropwise adding a sodium hydroxide aqueous solution into the mixed solution, carrying out hydrothermal reaction, cooling after the reaction is finished, centrifuging, washing and drying to obtain milk white powder, namely the amino-functionalized cerium oxide nano-enzyme. The boron element is introduced in the preparation of the cerium oxide nano-enzyme, and the amino-functionalized cerium oxide nano-enzyme is prepared by adopting a simple one-step hydrothermal synthesis method, so that the nano-enzyme has good phosphatase-like activity, has high selectivity and sensitivity to LPS, and is short in detection time, high in sensitivity, low in detection limit, simple in preparation process and high in preparation efficiency; the product is good in quality, high in stability, environment-friendly and extremely wide in market prospect.

The invention discloses an antibody for detecting indol sulfate, a detection kit and application of the antibody, and the antibody can be specifically combined with indol sulfate and has high combination activity. The detection kit detects the content of indoxyl sulfate in a to-be-detected sample through a competitive binding reaction of an immunomagnetic bead coated indoxyl sulfate antibody, an antigen in the sample and an alkaline phosphatase labeled indoxyl sulfate antigen, and has important economic value and social significance.

The invention provides an application of inositol monophosphate in an enzyme catalysis reaction system for preparing inositol. The invention also provides a preparation method of the inositol, the starch is used as a substrate, glucan phosphorylase, glucose phosphate mutase, inositol-3-phosphate synthase and inositol monophosphate are added as enzyme catalysts to catalyze the substrate to generate the inositol, the inositol monophosphate is derived from Archaeoglobus fulgidus, the amino acid sequence is shown as SEQ ID NO: 12, or the amino acid sequence is shown as SEQ ID NO: 1, or the amino acid sequence is shown as SEQ ID NO: 2, or the amino acid sequence is shown as SEQ ID NO: 3, or the amino acid sequence is shown as SEQ ID NO: 4, or the amino acid sequence is shown as SEQ ID NO: 5, or the amino acid sequence is shown as SEQ ID NO: 6. The inositol monophosphate is derived from Thermococcus kodakarensis, and the amino acid sequence of the inositol monophosphate is as shown in SEQ ID NO: 13; or the inositol monophosphate is derived from Pseudothermotoga lettinae, and the amino acid sequence of the inositol monophosphate is as shown in SEQ ID NO: 14. Inositol-3-phosphate synthetase and inositol monophosphate with good performance are screened, high-concentration starch is efficiently catalyzed to be converted into inositol by a one-pot method in a whole-cell feeding mode, and NAD < + > and other coenzymes do not need to be used. Under a 60L reaction system, 200g / L of corn starch is catalyzed, the unit yield of the prepared inositol reaches 161g / L, and the method has a good industrial application prospect.

The present invention relates to the technical field of the development and utilization of probiotics, specifically to a Weissella confusa DY12 with probiotic activity and its application. The Weissella confusa DY12 was isolated from sheep feces. The present invention identified the probiotic effect of Weissella confusa DY12 through animal experiments. The results showed that Weissella confusa DY12 could tolerate acid and bile salts and resist the gastrointestinal internal environment; its hydrophobicity was 39.47% and its auto-aggregation was 48.12%, demonstrating that the Weissella confusa DY12 of the present invention has good intestinal adhesion ability and has the potential of probiotics. The present invention verified that Weissella confusa DY12 can effectively regulate the immune function of the animal body, improve the antioxidant capacity of the body, as well as the content of lysozyme and the activity of alkaline phosphatase, and increase the daily weight gain, ultimately improving the growth performance of animals.

The invention belongs to the technical field of biological medicines, and particularly discloses application of (E)-BCI hydrochloride in preparation of a medicine for treating sepsis. According to the application of the (E)-BCI hydrochloride in preparation of the medicine for treating sepsis, by inhibiting the activity of bispecific phosphatase 6, a mitogen activated protein kinase signal channel is regulated and controlled, and the expression level of inflammatory factors IL-1beta and TNF-alpha is reduced. The invention discloses application of (E)-BCI hydrochloride in preparation of drugs for treating sepsis, BCI enters from a new target for regulating inflammation and immune balance through a targeted DUSP6 / MAPK signal channel, a non-antibiotic-dependent drug is provided for treatment of sepsis, and the (E)-BCI hydrochloride is especially suitable for systemic inflammatory response syndromes caused by infection, operations, wounds and other inducements, and has a wide application prospect. Wide clinical application prospects are realized.

The present invention relates to a pyrazine derivative, an application thereof in inhibiting SHP2, and a compound of formula (I) or pharmaceutically acceptable salts, esters, isomers, solvates, prodrugs or isotope labels thereof. The structure of the compound of formula (I) is as follows. The novel pyrazine derivative provided by the present invention has excellent inhibition of SHP2 activity and can be used to prevent and / or treat non-receptor protein tyrosine phosphatase-mediated or dependent diseases or disorders.

The invention discloses a compound microbial agent and application thereof in quality improvement of liquorice in saline-alkali soil in an arid region, and belongs to the field of microbial technology and agricultural planting. The compound microbial agent is prepared from arthrobacter globiforme, filamentous pseudomonas, sinorhizobium meliloti bacillus and bacillus subtilis; the viable count concentration of the compound microbial agent is (1-2) * 10 < 10 > CFU / mL. The compound microbial agent can remarkably improve the yield and quality of liquorice, and meanwhile, the compound microbial agent can improve the fertility of saline-alkali soil by activating soil phosphorus and potassium and improving the activity of urease and alkaline phosphatase. The invention solves the problems of weak glycyrrhiza rhizosphere colonization ability and low component conversion rate of the existing fungicide, and provides an innovative technical scheme for efficient glycyrrhiza planting in the saline-alkali soil in the arid region.

A Bifidobacterium longum subsp. longum dipro-017. The Bifidobacterium longum subsp. longum dipro-017 used has the ability to metabolize tryptophan to produce indole-3-lactic acid, can inhibit the pathogenic bacteria Salmonella Typhimurium, Staphylococcus aureus, and Escherichia coli, and can effectively mitigate weight loss caused by ulcerative colitis, improve a DAI score, improve colon length, mitigate the content of lipopolysaccharide (LPS) in blood, and increase the level of anti-inflammatory cytokine IL-10, as well as mitigate the content of bone metabolism indicators tartrate-resistant acid phosphatase (TRAP) and N-terminal propeptide of type I procollagen (PINP) in blood.

The disclosure relates to rare earth element (REE) phosphates and methods for making the same. The REE phosphates can be formed by combining a REE sulfate with a phosphate source and a biological catalyst, for example an acid phosphatase.

Featured are polypeptides that include soluble alkaline phosphatases, mutants, fragments, fusion proteins thereof, and methods of use thereof, for treating bone mineralization disorders, such as hypophosphatasia (HPP), and symptoms thereof. The polypeptides include a soluble alkaline phosphatase (sALP) or fragment thereof, which is derived from a naturally occurring alkaline phosphatase (ALP).

The invention discloses an eggshell membrane peptide for enhancing bone mineral density as well as a preparation method and application thereof, belongs to the technical field of egg by-product processing, and particularly relates to a preparation method of the eggshell membrane peptide for enhancing bone mineral density, which comprises the following steps: carrying out vacuum freeze drying and crushing on an eggshell membrane to obtain eggshell membrane powder; mixing the eggshell membrane powder with deionized water to obtain an eggshell membrane solution; adding keratinase, performing enzymolysis, and taking supernate; adding collagenase, carrying out enzymolysis, and centrifuging to take supernate, so as to obtain primary enzymatic hydrolysate; adding alkaline phosphatase, performing enzymolysis and centrifugation, and taking supernate to obtain enzymatic hydrolysate; and performing ultrafiltration and drying to obtain the eggshell membrane peptide. According to the eggshell membrane peptide for enhancing the bone mineral density, keratin cross-linking is destroyed through enzymolysis of keratinase, active peptide capable of stimulating osteoblast proliferation is released through enzymolysis of collagenase, phosphopeptide calcium binding sites are further activated through enzymolysis of alkaline phosphatase, calcium salt deposition is promoted, and the eggshell membrane peptide for enhancing the bone mineral density is provided.

The invention belongs to the field of microorganisms, and particularly provides a non-pathogenic penicillium ochrochloron strain which is derived from garlic rhizosphere soil, is classified and identified as penicillium ochrochloron, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC 41151. The strain has broad-spectrum bacteriostatic activity and can antagonize various plant pathogens; the plant growth promoting agent has the growth promoting characteristic, can secrete siderophores and phospholytic enzymes, and improves plant iron and phosphorus nutrition; the composition is safe and free of pathogenicity to crops. Tests show that the P4 strain can relieve plant iron deficiency yellowing and promote crop growth. The invention also provides application of the strain in biological control of plant diseases and plant growth promotion.

The present invention provides a compound of Formula (I) or a pharmaceutically acceptable salt tautomer, solvate, hydrate thereof. Also disclosed are methods of using such compounds and pharmaceutical compositions comprising such compounds. These compounds are useful in the treatment of proliferative disorders, such as cancer.

The invention discloses a nanofiber based on a polypeptide derivative and an enzymatic self-assembly method and application of the nanofiber based on the polypeptide derivative, the sequence of the polypeptide derivative is Nap-Gly-Phe-Phe-pTyr-Lys-Trp-Tyr-Gln-Asn-Met-Ile-Arg, and the Gly-Phe-Phe-pTyr is of an L configuration or a D configuration. The self-assembled polypeptide derivative disclosed by the invention contains an alkaline phosphatase restriction enzyme cutting site, and the self-assembled polypeptide derivative is self-assembled at a specific part with high enzyme specificity expression through enzyme catalysis to form nanofibers. The nanofiber hydrogel obtained through enzymatic treatment is carried out under physiological conditions, high temperature or ultraviolet irradiation is not needed, damage of high temperature to bioactive substances is avoided, the nanofiber hydrogel is more suitable for a temperature-sensitive in-vivo environment, toxic chemical cross-linking agents or photoinitiators do not need to be introduced, the cytotoxicity of the material is remarkably reduced, active enzymes or drugs can be embedded in situ, and the nanofiber hydrogel can be used for in-situ treatment. The biological function is maintained.

The present invention discloses a preparation method of an enhanced hydrolytic nanozyme for the degradation of organophosphorus pesticides. The broad-spectrum nucleic acid aptamer BSAPT is modified on the surface of a metal zirconium organic framework MOF-808 in various ways to form a MOF-808 / BSAPT or MOF-808-BSAPT enhanced hydrolytic nanozyme for selective and efficient degradation of organophosphorus pesticides. The enhanced hydrolytic nanozyme prepared by the present invention can simulate phosphatase activity and broadly identify a variety of highly toxic organophosphorus pesticides. The organophosphorus pesticide broad-spectrum nucleic acid aptamer BSAPT actively recognizes and captures organophosphorus compounds, enriches the target molecule to the Lewis acidic Zr(IV) active site center of MOF-808, and achieves selective and efficient degradation of organophosphorus pesticides by nucleophilic attack on the electrophilic group of the phosphorus atom center of the organophosphorus compound substrate. It can effectively degrade a variety of highly toxic organophosphorus pesticides such as chlorpyrifos, profenofos, and methyl parathion. The invention has simple preparation materials, low cost, fast degradation rate and broad spectrum, and can be applied to the degradation of various organophosphorus pesticides in water bodies.

The invention discloses a construction method and application of an electrochemical luminescence (ECL) biosensor based on a cell membrane hydrogel antifouling interface, and belongs to the technical fields of photo / electrochemical analysis, interface antifouling and biosensing. According to the invention, the cell membrane hydrogel is used as an efficient antifouling interface, so that non-specific adsorption of interfering proteins in a sample to be detected is hindered, and the service life of the biosensor is prolonged. Meanwhile, a luminophor copper nanosheet and a quencher MnO2 nanosheet are both introduced into the cell membrane hydrogel, in the presence of alkaline phosphatase (ALP), L-ascorbic acid-2-trisodium phosphate is catalyzed and hydrolyzed into ascorbic acid, and then MnO2 is reduced to recover ECL emission of the copper nanosheet. Therefore, the ECL signal of the biosensor is in a rising trend along with the rising of the ALP concentration. Based on this, the constructed ECL biosensor realizes sensitive detection of ALP, the linear range is 10 <-5 >-10 < 4 > U / L, and the detection limit is as low as 3.4 * 10 <-6 > U / L, which indicates that the ECL biosensor has potential application in clinical diagnosis.

The invention discloses a method for detecting glyphosate residues in coffee in fluorescence and chemiluminescence dual modes, and belongs to the technical field of chemical analysis and detection.The method comprises the steps that succinic acid serves as a ligand, Ce < 4 + > serves as a central ion, a Suc-Ce complex is synthesized through a microwave method, then the Suc-Ce complex is further subjected to alkali treatment to obtain Suc-Ce-OH nano-enzyme, and the Suc-Ce-OH nano-enzyme is used for detecting glyphosate residues in coffee. The Suc-Ce-OH nano-enzyme has excellent phosphatase-like activity, surface coordinated water molecules can be effectively removed through alkali treatment, more Ce-O active sites are exposed, and the hydrolysis efficiency of the Suc-Ce-OH nano-enzyme on a fluorescent substrate 4-MUP and a chemiluminescent substrate AMPPD is improved by two times; based on the specific inhibition effect of glyphosate on phosphatase-like nano-enzyme activity, a glyphosate fluorescence and chemiluminescence dual-mode detection method is established, the detection limits of the glyphosate fluorescence and chemiluminescence dual-mode detection method are 0.05 mu g / mL and 0.01 mu g / mL respectively, and the glyphosate fluorescence and chemiluminescence dual-mode detection method is used for detecting glyphosate residues in a coffee sample and has the advantages of being high in specificity, easy to operate and sensitive.

The invention belongs to the field of metabolic engineering, and discloses a genetically engineered bacterium for producing myo-inositol as well as a construction method and application of the genetically engineered bacterium. The genetically engineered bacterium takes escherichia coli as an original strain, and is obtained by performing the following gene editing on a genome of the escherichia coli: non-expressed lactose operon repressor protein, glucose phosphate dehydrogenase, acetokinase, glucose phosphate isomerase and glycerol repressor protein; and overexpressing inositol-1-phosphate synthase, inositol monophosphate, glucokinase, glucose permease and glycerol kinase. Compared with the prior art, the genetically engineered bacterium disclosed by the invention has remarkable advantages in the aspects of key enzyme expression rate, metabolism specificity, fermentation period and the like, and an efficient, stable, economical and feasible solution is provided for industrial production of myo-inositol.