24 results about "Host organism" patented technology

Described herein are biocatalytic methods of producing terpene degradation products useful as starting material for the production of perfumery ingredients, such as, for example, ambrox. In particular novel terpene degrading polypeptides (enal-cleaving polypeptides) and novel peptides converting terpenes compounds to oxygenated derivatives (oxygenases) and mutants and variants derived therefrom are described which may be applied in novel types of fully enzymatic multistep degradation pathways allowing the controlled, stepwise conversion and degradation of linear or cyclic terpene substrates. Said novel biosynthetic strategies allow the fully biochemical synthesis of valuable terpene-derived compounds, like for example manooloxy or gamma ambrol. Also described herein are recombinant host organisms carrying the required set of genetic information for the functional expression of the set of enzymes necessary for catalyzing the combination of enzymatic conversion and degradation steps.

The invention relates to the substances benfo-oxythiamine and / or oxythiamine for use as the active ingredient of a topically administered medicament for the therapeutic treatment of patients with a disease of the body surface skin (cutis and subcutis) and / or one or more mucous membranes (mucosa) caused by viruses. The medicament is administered at a time when viral replication has already taken place and viral release from the host cell(s) is imminent.Benfo-oxythiamine and / or oxythiamine cause (I) a slowing or prevention of the spread mechanisms of the viruses, so that the viruses remain in their host cells after replication and cell damage in the patient organism (host organism) is reduced or prevented, and (II) an immune response against the viruses remaining in the cells, so that the patient's immune system is activated or reactivated to generate an effective immune response against the viruses.

Disclosed herein are methods and compositions related to probiotic formulas. These formulas comprise the bacterial strain Lactobacillus crispatus WZ-12 or a derivative thereof. Other strains which can be included in the composition include, but are not limited to, Lactobacillus salivarius 1-14, or Lactobacillus reuteri 2-2, and derivatives thereof. These compositions can be used in a variety of methods to treat and / or ameliorate diseases, such as colonization of antibiotic-resistant bacteria in the host organism. Also disclosed herein are methods of treating those subjects in need thereof by administering the compositions disclosed herein.

A process for the production of a membrane protein is provided. The process including the steps of: expressing a membrane protein in a host organism present in an aqueous medium, liberating the membrane protein from the host organism, adding a detergent solution to solubilize the membrane protein, recovering a liquid fraction of the solubilized membrane protein, subjecting the liquid fraction to chromatography to bind or retain the membrane protein on a stationary phase, and eluting the stationary phase with an elution buffer to produce the membrane protein. The process can produce relatively large amounts of membrane proteins in an efficient way without compromising the quality of the end product.

Disclosed herein are methods for producing interfering RNA biopesticides, such as microbial host organisms engineered to produce interfering RNA (iRNA) molecules which selectively undermine the survival of cockroaches. Such iRNA molecules selectively inhibit the expression of a gene in a specific insect population, such as a cockroach population, by RNA interference. Also disclosed herein are polynucleotides, such as expression cassettes encoding iRNA molecules and facilitating integration, such as stable integration into the genome of a host. Further disclosed herein are compositions including the disclosed nucleotide sequences and host organisms, along with methods of using the same to control cockroach populations.

This invention discloses a mass spectrometry patch for wound infection detection and its application, relating to the field of biomedical device technology. The mass spectrometry patch consists of a contact layer, a collection layer, and a protective layer from bottom to top. The contact layer is a polycarbonate mesh film, the collection layer is a polyvinylidene fluoride nanofiber membrane with high-density gold nanoparticles grown in situ on its surface, and the protective layer is a polyurethane film. This invention provides a wearable mass spectrometry patch integrating sampling and detection, achieving non-invasive sampling and direct mass spectrometry analysis. A single detection using laser desorption / ionization mass spectrometry can obtain a global metabolic fingerprint containing hundreds of metabolites, which simultaneously contains pathogen species-specific signals and host metabolic biomarker signals. By training an AI model to analyze the complex fingerprint, rapid pathogen identification and dynamic monitoring of host responses (such as lactic acid and glucose) can be completed simultaneously within 5 minutes, achieving decoupling of pathogen information from changes in host biomarkers.

The invention relates to the substances benfo-oxythiamine and / or oxythiamine for use as an active ingredient of a medicament to be administered topically for the therapeutic treatment of patients with a disease of the body surface skin (cutis and subcutis) and / or one or more mucous membranes (mucosa), said disease having been triggered (caused) by viruses. The medicament is administered at a point in time at which viral replication has already taken place and viral shedding from the host cell(s) is imminent. Benfo-oxythiamine and / or oxythiamine has / have the following effects: (I) a throttling or prevention of the propagation mechanisms of the viruses so that the viruses remain in the host cells thereof after replication has taken place and cell damage to the patient organism (host organism) is reduced or does not occur and (II) an immunological reaction to the viruses remaining in the cells so that the patient's immune system is activated or reactivated in order to generate an effective immune response to the viruses.

The present invention introduces an innovative approach for the manipulation of microorganisms to generate colored bioplastics. This is achieved by concurrently expressing genes responsible for pigment production and genes involved in bioplastic synthesis within a microbial host. These genes can be synthesized, obtained from a different host through cloning, or naturally occurring within the host organism. The resultant color compounds become encapsulated within the extended bioplastic polymers within the cells, resulting in the formation of naturally pigmented bioplastics.

The invention relates to a tuberculosis antigen specific host biomarker for detecting tuberculosis infection and a kit, and belongs to the field of biological medicines. The biomarker comprises IFN gamma, CXCL10, IL-2, IL-3, GBP6, ULBP2, CXCL11, ATP13A3, RHOV, ATP2B1, IL-22 and MMP1 (Metallocin Protein Protein 1). According to the present invention, the specific RD1 region antigen or peptide pool from mycobacterium tuberculosis and the specific RD2 region antigen or peptide pool from mycobacterium tuberculosis are jointly used as the antigen irritants to stimulate peripheral whole blood or peripheral blood mononuclear cells (PBMCs) of an individual to be detected, and then the expression quantity change of the host biomarker before and after the stimulation is detected; the kit can effectively distinguish tuberculosis infected patients from non-tuberculosis infected individuals, can identify and diagnose active tuberculosis and latent tuberculosis infected patients, solves the defects of insufficient sensitivity and limited specificity of the existing diagnostic technology, and has high diagnostic efficiency.

The application provides a method for improving biological stress resistance by using a dotP gene, which comprises the following steps: transferring the dotP gene into a host organism, and making the host organism express the dotP gene; the host organism is a plant or a microorganism; the dotP gene encodes a protein (a) or (b): (a) a protein consisting of the amino acid sequence shown in SEQ ID NO. 2; (b) a protein derived from (a) by substituting, deleting or adding n amino acids in the amino acid sequence in (a) and having the activity of a quadruplet repeat sequence protein, wherein n is any integer between 1 and 4. By the technical scheme, the biological stress resistance of the plant or the microorganism is improved, and in particular, the dry resistance of the plant or the microorganism is improved.

The invention relates to methods for detecting and quantifying the presence of an organism in a sample, typically for detecting and quantifying the presence of a non-host organism in a host, and kits providing components useful for performing such methods. The invention also relates to methods for monitoring the effectiveness of a treatment of a disease or infection associated with a pathogen in a host. The invention further relates to methods for treating a disease or an infection associated with a pathogen in a host. The invention additionally relates to a computer program and to a computer-readable storage medium, comprising instructions causing a computer to carry out the method of the invention.

The present invention provides the nucleic acid and amino acid sequences of a cytochrome P450 capable of oxidizing terpene molecules. It also provides methods for oxidizing terpene molecules, comprising contacting the cytochrome P450 with the terpene molecule to be oxidized. In particular, the method can be carried out in vitro or in vivo to produce oxidized terpene molecules, which can be used in various technical fields such as, for example, perfumery and flavorings. The present invention also provides an expression vector containing the nucleic acid. A non-human host organism or a cell transformed with the nucleic acid is also an object of the invention.

A composition comprising a recombinant probiotic organism engineered to constitutively express an enzyme involved in maintaining telomere length, wherein the enzyme comprises a telomerase subunit, the genetic modification comprises an operon comprising a gene sequence encoding telomerase having a promoter recognized by the RNA polymerase of the probiotic organism and a genetic modification that induces the secretion of telomerase into the host organism, and the genetic modification of the telomerase gene sequence enhances the entry of the telomerase enzyme into the host organism's cells.

This document discloses methods for producing interfering RNA (RNA) biopesticides, such as microbial host organisms engineered to produce interfering RNA molecules that inhibit gene expression in disease-carrying mosquitoes through RNA interference. This document also discloses polynucleotides, such as expression cassettes encoding these interfering RNA molecules and facilitating integration (e.g., stable integration) into the host organism's genome. This document further discloses compositions comprising the disclosed nucleotide sequences and a host organism, and methods for using them to control mosquito populations.

The invention relates to a tuberculosis antigen-specific host biomarker for diagnosing tuberculous pleural effusion and a kit, and belongs to the technical field of biological medicines. The host biomarker provided by the invention comprises the following components: IFN (interferon) gamma, IL-2 (interleukin-2), IL-12RB2 (interleukin-12R < 2 >), GZMB (Growth Zehnder Microorganism Blanket), IL-3 (interleukin-3), CXCL10 (C X-ray cell line 10), IL-12B (interleukin-12B), IL-18RAP ( According to the present invention, the mycobacterium tuberculosis RD1 region antigen or peptide fragment and the mycobacterium tuberculosis RD2 region antigen or peptide fragment are jointly used as the antigen irritants to stimulate the pleural effusion sample to be detected, and then the expression change of the host biomarker is detected so as to effectively identify and diagnose the tuberculous pleural effusion. By combining tuberculosis specific antigen stimulation and local infection microenvironment transcriptome analysis, the non-specificity defect of an existing host biomarker is overcome, and the host biomarker has high sensitivity and specificity in diagnosis of tuberculous pleural effusion.

Disclosed herein are methods for producing interfering RNA larvicides for Spotted Wing Drosophila, D. suzukii, such as microbial host organisms engineered to produce interfering RNA (iRNA) molecules which selectively undermine the survival of female D. suzukii larva, to produce a predominantly male population of adult SWD flies. Such iRNA molecules selectively inhibit the expression of a gene in female SWD larvae population by RNA interference. Also disclosed herein are polynucleotides, such as expression cassettes encoding iRNA molecules and facilitating integration, such as stable integration into the genome of a host cell. Further disclosed herein are compositions including the disclosed nucleotide sequences and host organisms, along with methods of using the same to generate a predominately male SWD population.

A method for assaying putative Psilocybe spores, Psilocybe tissue, or a cultured host organism that expresses Psilocybe genes comprises obtaining a sample including nucleic acids, contacting the sample with primers for amplifying target nucleic acid sequences, amplifying any of the target nucleic acid sequences when present among the nucleic acids to obtain amplicons, and detecting the amplicons upon amplification of the target nucleic acid sequence. In embodiments, the primers include primers for amplifying (i) a bacterial target nucleic acid sequence, (ii) a Psilocybe target nucleic acid sequence, and / or (iii) a fungal target nucleic acid sequence. For instance, the primers include primers for amplifying a Psilocybecubensis target nucleic acid sequence and a target nucleic acid sequence from at least one other mushroom species subject to misidentification as Psilocybecubensis. A kit may include those primers and probes for detecting the amplicons amplified from any of those target nucleic acid sequences.

In some other conventional carminic acid production methods, carminic acid production may be carried out within a host organism and / or cells. The present invention relates to materials and methods for the production of carminic acid from substrates. The present invention provides methods and materials for cell-free bioproduction of carminic acid. The methods provided in the present invention relate to cell-free production of carminic acid that is more economical and reliable compared to other conventional carminic acid production methods. Furthermore, the methods provided in the present invention provide carminic acid from these processes that offers higher potency compared to carminic acid produced by other conventional methods.

A composition comprising a recombinant probiotic organism reengineered to constitutively express an enzyme involved in the maintenance of telomere length wherein the enzyme comprises a telomerase subunit, the genetic modification comprising an operon, a telomerase subunit, a telomerase subunit, a telomerase subunit, a telomerase subunit, a telomerase subunit, a telomerase subunit, a telomerase subunit, and a telomerase subunit. The operon comprises a promoter recognized by an RNA polymerase of the probiotic organism and a gene sequence encoding a telomerase, has a genetic modification that induces secretion of the telomerase into the host organism, and the genetic modification of the telomerase gene sequence enhances telomerase entry into cells of the host organism.

Described herein are biocatalytic methods of producing terpene degradation products useful as starting material for the production of perfumery ingredients, such as, for example, ambrox. In particular, terpene degrading polypeptides (enal-cleaving polypeptides) and peptides converting terpenes compounds to oxygenated derivatives (oxygenases) and mutants and variants derived therefrom are described which may be applied in fully enzymatic multistep degradation pathways allowing the controlled, stepwise conversion and degradation of linear or cyclic terpene substrates. The biosynthetic strategies allow the fully biochemical synthesis of valuable terpene-derived compounds, like for example manooloxy or gamma ambrol. Also described herein are recombinant host organisms carrying the required set of genetic information for the functional expression of the set of enzymes necessary for catalyzing the combination of enzymatic conversion and degradation steps.

An L-threonine dehydratase variant is provided that is capable of producing increased yields of the amino acid L-isoleucine when expressed in host organisms and methods of producing increased yields of L-isoleucine using the same.

Provided herein are biocatalytic methods for the production of terpene degradation products that can be used as starting materials for the production of fragrance ingredients such as ambrox. In particular, novel terpene degradation polypeptides (enal cleavage polypeptides) and novel peptides that convert terpene compounds to oxidized derivatives (oxidases) are provided, as well as mutants and variants derived therefrom, which can be applied in novel, fully enzymatic multi-step degradation pathways, allowing controlled, stepwise conversion and degradation of linear or cyclic terpene substrates. The novel biosynthetic strategies allow fully biochemical synthesis of valuable terpene-derived compounds such as minorenoloxyl or gamma-ambrol. The present invention also provides recombinant host organisms that carry the required set of genetic information for the functional expression of the set of enzymes necessary for the combination of enzymatic conversion and degradation steps.

Disclosed herein are methods for producing interfering RNA biopesticides, such as microbial host organisms engineered to produce interfering RNA (iRNA) molecules which selectively undermine the survival of lepidopteran insects, such as agricultural pest moths and larval (caterpillar) forms thereof. Such iRNA molecules selectively inhibit the expression of a gene in a specific insect population, such as a lepidopteran population, by RNA interference. Also disclosed herein are polynucleotides, such as expression cassettes encoding iRNA molecules and facilitating integration, such as stable integration into the genome of a host cell. Further disclosed herein are compositions including the disclosed nucleotide sequences and host organisms, along with methods of using the same to control lepidopteran populations.

The present disclosure provides patterned biomaterials having organized cords and extracellular matrix embedded in a 3D scaffold. According, the present disclosure provides compositions and applications for patterned biomaterials. Pre-patterning of these biomaterials can lead to enhanced integration of these materials into host organisms, providing a strategy for enhancing the viability of engineered tissues by promoting vascularization.