100 results about "Dna encoding" patented technology

Provided are an antigenic peptide having multiple KRAS variant peptides linked thereto, mRNA encoding same, DNA encoding same, an immunogenic composition comprising an antigenic peptide having multiple KRAS variant peptides linked thereto or an mRNA encoding same, and a method for inducing an immune response to the KRAS variant peptides in a subject.

The present invention relates to a recombinant human type IV collagen and a preparation method therefor, which belong to the technical field of bioengineering. A recombinant human type IV collagen is designed on the basis of a partial sequence in an α2 chain of human type-IV collagen, and DNA encoding of the protein is ligated into an expression vector to construct a recombinant expression vector; then the recombinant expression vector is transformed into competent Escherichia coli cells, and a recombinant expression plasmid is extracted; after the plasmid is linearized, the linearized plasmid is transformed into an engineered strain HCPB-PPKEX2 to obtain a recombinant engineered strain; and the engineered strain is cultured, the expression of a target protein is induced, and the target protein is purified and identified. It is further demonstrated experimentally that the collagen can be completely cleaved by Kex2, Ste13 and CPB enzymes and efficiently secreted and expressed extracellularly, and the obtained sequence is completely consistent with a theoretical sequence. It is also verified experimentally that the recombinant collagen has a cell adhesion activity and a cell migration-promoting activity. The method avoids the risk of residual exogenous proteins, shortens the time and reduces the cost of the subsequent purification process, and thus has a relatively high safety and application value.

The present invention particularly relates to the technical field of data storage, and provides a data encryption method based on DNA encoding, a data decryption method based on DNA encoding, an intelligent terminal, and a medium. A solution comprises: encrypting acquired data to be encoded to obtain encrypted data to be encoded; on the basis of a pre-constructed DNA encoding table, performing DNA encoding on each byte of said encrypted data to obtain an initial base sequence, the DNA encoding table being constructed from four-base units and five-base units; and using a preset DNA sequence structure optimization rule to perform chaotic mapping adjustment and rearrangement on the initial base sequence to obtain an encrypted DNA sequence. According to the solution, the GC content in the encrypted DNA sequence can be kept in a balanced state, and the information density of the encrypted data is increased, such that the encryption effect is effectively improved, thereby reducing a storage space occupied by data to be encoded, improving the security and attack resistance of the data to be encoded, and thus guaranteeing the stability of the encrypted DNA sequence.

The invention discloses an encryption transmission method and system for financial transaction data and a storage medium, and the method comprises the steps: carrying out the grading preprocessing of original financial transaction data through an amount sensitivity grading algorithm, and obtaining a four-stage transaction amount data set and an account feature code; initializing 8D financial chaotic parameters for the numerical values and the account feature codes to obtain an eight-dimensional chaotic parameter sequence; performing frequency domain decomposition on the fourth-level transaction amount data set to obtain four pieces of frequency domain sub-band data; dynamically generating a DNA coding rule according to the account feature code and the eight-dimensional chaos parameter sequence to obtain a dynamic DNA coding mapping table; and performing adaptive block scrambling on the four frequency domain sub-band data according to the amount level, and performing fusion processing through a dynamic DNA coding mapping table to obtain an encrypted data packet. The technical problem that an existing financial transaction data encryption transmission method cannot perform differential security protection according to transaction amount sensitivity and lacks anti-quantum computing capability is solved.

The disclosure relates to antibodies specific to FcRn, formulations comprising the same, use of each in therapy, processes for expressing and optionally formulating said antibody, DNA encoding the antibodies and hosts comprising said DNA.

The presently disclosed subject matter relates to the use of a haploid inducing line transformed such that it contains DNA encoding a cellular mechanism capable of editing a gene. This mechanism is preferentially expressed in zygotic cells. Exemplary promoters include, but are not limited to, a VSP promoter, an RZDP promoter, an SCE1 promoter, and a U3 promoter. The promoters can be obtained from arabidopsis thaliana, corn, soybean, wheat, sunflower, rice and tomato.

To provide recombinant herpes simplex virus-2 (HSV-2) vaccine vectors and compositions and vaccines containing them, and methods of use thereof.SOLUTION: Disclosed is a process for producing a vaccine vector targeting a heterologous antigen, the process comprising the steps of: providing a HSV-2 genome nucleic acid comprising: (i) a gene encoding an intact or partially deleted HSV-2 glycoprotein D and (ii) a promoter-FP construct; b) co-transfecting a host cell with: (i) the HSV-2 genome and (ii) a linear DNA encoding the heterologous antigen under conditions where allelic recombination will occur between the HSV-2 genome of a) and the DNA fragment; c) screening plaques resulting from b) and identifying plaques not showing fluorescence under excitation light which elicits fluorescent protein fluorescence; and d) recovering a recombinant HSV-2 virus or virion from plaques not showing fluorescence in c).SELECTED DRAWING: Figure 2

The present application relates to a DNA encoding method, decoding method, device, electronic device and medium. The method includes: obtaining binary data to be encoded; grouping the binary data to obtain a plurality of binary data groups; for each binary data group, when the first binary bit of the binary data group is 0, obtaining a preset first mapping table; encoding the binary data group into corresponding base units according to the preset first mapping table; when the first binary bit of the binary data group is 1, obtaining a preset second mapping table; encoding the binary data group into corresponding base units according to the preset second mapping table; merging the corresponding base units of each binary data group to obtain a base sequence corresponding to the binary data, thereby improving the accuracy of DNA synthesis and sequencing.

The disclosure relates to antibodies specific to FcRn, formulations comprising the same, use of each in therapy, processes for expressing and optionally formulating said antibody, DNA encoding the antibodies and hosts comprising said DNA.

The embodiment of the invention discloses an image encryption method and system based on a fractional order pseudo-random number generator and DNA coding and decoding, and the method comprises the steps: determining the length of a key string required for encryption based on a partitioned image, and generating a high-randomness key string according to the fractional order pseudo-random number generator, the problems that a traditional integer order chaotic system is insufficient in randomness and limited in secret key space are solved, a CBC chain type encryption mode is introduced to conduct inter-block encryption on each block of image after block division, generation of each ciphertext block depends on a previous ciphertext, inter-block relevance is established, the problem that the inter-block relevance is missing due to the independent encryption characteristic is solved, and the encryption efficiency is improved. According to the method, the image is encrypted, the differential attack resistance of the image is enhanced, the DNA coding and decoding rule is dynamically selected based on the key string, and the image block is encrypted on the basis, so that the defect that the static coding rule is easily subjected to exhaustive attack is overcome, the real-time dynamic coding mechanism is realized, and the attack resistance of image encryption is improved.

The invention belongs to a DNA storage method, and provides a DNA encoding method, a DNA decoding method and a related device for solving the technical problems that a compression ratio does not reach an optimal state and the storage efficiency and the information density are low in a compression technology in the technical field of DNA information storage at present. Besides, when decoding is carried out after the super-resolution model is recovered and stored by adopting the storage object, the storage quality of the storage object can also be ensured, and an effective lossy compression algorithm based on DNA information storage is formed. Moreover, in practical application, the method can be combined with any DNA coding and decoding algorithm, so that other functions can be expanded on the basis of lossy compression, such as single base repetition or GC content of a control sequence. Therefore, on the premise of ensuring the decoding effect of the storage object, the storage density of the DNA information of the storage object is effectively improved, and the synthesis cost is reduced.

The invention discloses an image encryption method based on phase-shift digital holography and a multi-mode biological characteristic key, which comprises the following encryption steps of: firstly, encrypting a grayscale image into a DNA compilation result by using DNA coding and an iris chaos mask; secondly, encrypting a DNA compiling result into three ciphertext holograms by using phase-shift digital holography and fingerprint and finger vein chaos masks; the decryption step comprises the following steps of: firstly, authenticating three biological characteristics of decrypted iris, fingerprint and finger vein; if the authentication is successful, the iris chaos mask and the fingerprint and finger vein conjugate chaos mask for subsequent decryption are generated by the system. And finally, obtaining a final decrypted image by using a digital holographic reconstruction technology and a DNA decoding technology. The method provided by the invention has the advantages of high security level, high biological key robustness and high digital key sensitivity.

The preparation of a library of bispecific binders expressed in eukaryotic cells described herein is a method for producing a cloned library of eukaryotic cells containing DNA encoding a diverse library of bispecific binders, the present invention relates to bispecific conjugates, in particular bispecific conjugates comprising a first binding domain coupled to a first Fc domain and a second binding domain coupled to a second Fc domain.

This invention discloses an attack tracing method and system for end-to-end data transmission in optical communication, belonging to the field of optical fiber communication technology. The method includes: processing an initial key value using a hash function and DNA encoding to generate a DNA sequence; concatenating the DNA sequence to generate a session key and calculating its hash value; calculating a single-byte XOR checksum of the original data to generate extended data, generating ciphertext based on the extended data, and calculating a digest of the ciphertext; decrypting the ciphertext and performing XOR checksum consistency verification to obtain an event digest; calculating a root hash based on the key digest, parameter digest, ciphertext digest, and event digest, storing the root hash in the block header, and performing attack tracing on the block header to obtain the attack tracing result. This invention introduces four-dimensional chaos and DNA encoding to generate a high-entropy key, dynamically storing and updating the key through blockchain, while simultaneously achieving single-root verifiable attack tracing and quickly locating problematic nodes.

The invention relates to the technical field of DNA encryption, and discloses a DNA encryption method and device based on a random combination code table, equipment and a storage medium, and the method comprises the steps: obtaining seven designed code tables with different sizes and a to-be-coded file; generating a random number according to the file type of the to-be-coded file; carrying out code table selection in each code table based on the random number, taking the selected code table as a target code table, then carrying out coding conversion according to the target code table and the to-be-coded file to obtain a DNA coding result, and generating a random sequence by using chaotic mapping based on the length corresponding to the DNA coding result; and adjusting the sequence of the bases in the DNA coding result according to the random sequence to obtain a randomized DNA encryption sequence, and performing code conversion on the to-be-coded file through the code table composed of the screened bases to obtain the DNA encryption sequence, thereby realizing DNA encryption of the to-be-coded file, and improving the security and efficiency of encryption.

Provided herein is a protein from Tumebacillus sp. NITE BP-02779 having a peptidoglycan-decomposing activity. Provided herein is a protein comprising the amino acid sequence of SEQ ID NO: 2 (1-164) or the amino acid sequence of SEQ ID NO: 4 (1-493), or a protein comprising an amino acid sequence in which 1 to 10 amino acid residues of these amino acid sequences are substituted, deleted, inserted, or added, or an amino acid sequence having 90% or more identity to these amino acid sequences, and having a peptidoglycan-decomposing activity.

Synthetic bacterial messenger RNA can be used to make autologous, allogeneic or direct nucleic acid cancer vaccines. Cancer cells are transfected in vitro or in vivo with mRNA obtained from DNA encoding immunogenic bacterial proteins. The immune response to cancer is generated by direct administration of the mRNA in vivo or administration of a vaccine made from cancer cells in vitro. Codon modification of the mRNA can optimize expression of the immunogenic polypeptide in cancer cells.

The present invention relates to a DcR3 variant, DNA encoding the DcR3 variant, a vector containing the DNA, a transformant obtained by introducing the vector, a method for producing a variant using the transformant, and a prophylactic or therapeutic agent for an autoimmune disease, an inflammatory disease or an allergic disease comprising the variant as an active ingredient. The present invention relates to a DcR3 variant having binding activity (preferably neutralizing activity) against a ligand of DcR3 while showing reduced production of aggregates and / or improved in vivo kinetics compared to wild-type DcR3 when prepared with a mammalian-derived cell as a host, and provides a DcR3 variant comprising a portion of DcR3 and a portion of a TNF superfamily molecule.

The present invention relates to a phage-based gene delivery system targeting human cells or animal cells, including immune cells, other human cells and cancer cells. In particular, the present invention relates to novel phage particles and associated phagemid expression systems and their production for the delivery of transgenes, comprising DNA encoding pathogen antigens, to human immune cells, suitable for use in vaccines and vaccine development against infectious diseases and cancers.

The present invention relates to the field of biotechnology and proposes antibodies that bind specifically to PD-L1. Furthermore, the discovery relates to the DNA encoding these antibodies, the corresponding expression vectors and production methods, as well as treatment methods that utilize these antibodies.

The purpose of the present invention is to provide a means for producing a non-animal-derived modified heparosan compound with higher efficiency than conventional means. Provided is any one of the following proteins [1] to [3] fused to a TRX tag. [1] A protein which comprises the amino acid sequence represented by SEQ ID NO: 1. [2] A protein which comprises an amino acid sequence having a structure wherein 1 to 20 amino acid residues are deleted, substituted, inserted, or added in the amino acid sequence represented by SEQ ID NO: 1 and which has N-sulfotransferase activity. [3] A protein which comprises an amino acid sequence having 70% or more identity to the amino acid sequence represented by SEQ ID NO: 1 and has N-sulfotransferase activity.

The present application provides an extended coding method for a storage channel, including: obtaining binary data and decomposing it into binary source blocks, encoding it using a preset code to obtain an independent data source block; using a preset encoder to encode the source symbol sequence in the independent data source block to obtain a coding sequence that does not contain homopolymers; using automatic indexing to generate a primary data source block index and a secondary source symbol sequence index, compounding them in a DNA coding symbol sequence to obtain a base index; mapping the DNA coding symbol sequence to a binary symbol sequence, and inversely mapping it to a DNA sequence after adding controlled redundancy through RS error correction coding. The present application breaks through the theoretical information density of 2 bits / Base of DNA data coding with natural bases as basic storage units, and realizes efficient, reliable and practical DNA coding. The present application also provides an extended coding system, a computer-readable storage medium and an electronic device for a storage channel, which have the above-mentioned beneficial effects.

The present invention relates to a mutated SARS-COV-2 spike protein, a variant or fragment thereof or an mRNA or DNA encoding them for use in the prevention of COVID-19.

The application relates to the technical field of communication, in particular to a chaotic image encryption method based on global dynamic selection, which has the technical scheme as follows: S1, generating a chaotic sequence used in the encryption method by using a 2D-LSM chaotic system; S2, expanding an image into a one-dimensional array through raster scanning; S3, dynamically DNA encoding the image to convert the one-dimensional array into a DNA matrix; S4, dynamically permuting the DNA matrix to obtain a permutation image; S5, DNA decoding the permutation image to obtain a DNA decoding matrix; S6, dynamically diffusing and encrypting the DNA decoding matrix to obtain an encrypted image; and S7, repeating steps S2-S6 to repeatedly encrypt the encrypted image as an initial image. The method has the advantages of high flexibility and selectivity.

The present invention provides compounds and methods targeting insulin-like peptide-5 (INSL5), including antibodies, cells and vectors comprising DNA encoding the same, and methods for producing the antibodies. In addition, the present invention provides for use of INSL5 antibodies in diagnostic assays.

The invention discloses an image encryption method based on fractional order heterogeneous neurons and DNA dynamic coding, and the method comprises the steps: 1) dividing an original color image into a two-dimensional matrix, and carrying out the partitioning processing; 2) constructing a fractional order heterogeneous neuron model; 3) generating a plurality of groups of chaotic sequences and dynamic keys; 4) dynamically selecting a DNA coding rule and an operation rule; 5) continuing to dynamically select the DNA decoding operation rule, and performing DNA decoding on the block matrix; 6) performing replacement operation on the three two-dimensional matrixes; and 7) generating a ciphertext image, combining the two-dimensional matrixes of the three channels after the operation in the steps, and generating an encrypted ciphertext image. The invention further discloses an image decryption method based on the fractional order heterogeneous neurons and DNA dynamic coding. The invention belongs to the technical field of information encryption, and solves the problems of poor security, low processing efficiency and insufficient dynamic adaptability of the existing image encryption technology.

The present invention discloses a color image encryption method based on a hyperchaotic system and multi-stage scrambling and diffusion at the octabase DNA level, belonging to the technical field of digital image encryption. The method generates a hyperchaotic sequence through a hyperchaotic system, extracts a subsequence and adjusts the numerical range; converts the plaintext color image from the pixel level to the bit level, and then converts it to the octabase DNA level according to the hyperchaotic subsequence and dynamic octabase DNA encoding; performs a global scrambling of the row and column levels on the octabase DNA level data through the sorting index of the hyperchaotic subsequence; based on multiple different hyperchaotic subsequences, uses octabase DNA algebraic operations and cyclic shifts to perform row diffusion, column scrambling, column diffusion and row scrambling in sequence; then uses dynamic octabase DNA decoding to convert back to the bit level, and then converts to the pixel level to obtain the ciphertext image. The present invention improves the security of encrypting color images through octabase DNA calculation and multi-stage scrambling and diffusion operations.

The present invention relates to the field of bioengineering and provides a monoclonal antibody that specifically binds to CD20. The present invention also relates to DNA encoding the antibody, a corresponding expression vector and a method for producing the same, and a therapeutic method using the antibody.

A method for nucleic acid detection based on a ternary DNA coding library belongs to the technical field of in vitro nucleic acid detection. The method aims to quickly determine whether a specific gene fragment is present in a solution through the coding library. By setting a certain number of nucleic acid structures in the coding region on the carrier, the size and number of nucleic acid structures in different locations determine the intensity of the nanopore electrical signal, thereby forming a multi-level electrical signal, and then creating a multi-binary coding library. Probes and marker nucleic acid structures are set in the sensing area of ​​the carrier to detect specific gene fragments. This method is based on DNA data coding structure for library construction and is an emerging rapid detection method with the characteristics of high sensitivity and high throughput.

The invention discloses a DNA encoding method and device and a DNA decoding method and device, and belongs to the technical field of information storage and security. The DNA encoding method comprises the steps that target information is converted into computer codes; converting the computer code into a DNA coding chain or a DNA coding chain combination; and cross-linking the DNA coding chain and / or the DNA coding chain combination in a storage micro-pool on a DNA information storage medium so as to realize the storage of the target information. According to the method, the information storage process is completely independent of DNA synthesis and DNA sequencing technologies, and the method has the advantages of high parallelism, short access time, good encryption, low cost, strong expansibility and the like.