50 results about "Dna encoding" patented technology

The presently disclosed subject matter relates to the use of a haploid inducing line transformed such that it contains DNA encoding a cellular mechanism capable of editing a gene. This mechanism is preferentially expressed in zygotic cells. Exemplary promoters include, but are not limited to, a VSP promoter, an RZDP promoter, an SCE1 promoter, and a U3 promoter. The promoters can be obtained from arabidopsis thaliana, corn, soybean, wheat, sunflower, rice and tomato.

The disclosure relates to antibodies specific to FcRn, formulations comprising the same, use of each in therapy, processes for expressing and optionally formulating said antibody, DNA encoding the antibodies and hosts comprising said DNA.

The embodiment of the invention discloses an image encryption method and system based on a fractional order pseudo-random number generator and DNA coding and decoding, and the method comprises the steps: determining the length of a key string required for encryption based on a partitioned image, and generating a high-randomness key string according to the fractional order pseudo-random number generator, the problems that a traditional integer order chaotic system is insufficient in randomness and limited in secret key space are solved, a CBC chain type encryption mode is introduced to conduct inter-block encryption on each block of image after block division, generation of each ciphertext block depends on a previous ciphertext, inter-block relevance is established, the problem that the inter-block relevance is missing due to the independent encryption characteristic is solved, and the encryption efficiency is improved. According to the method, the image is encrypted, the differential attack resistance of the image is enhanced, the DNA coding and decoding rule is dynamically selected based on the key string, and the image block is encrypted on the basis, so that the defect that the static coding rule is easily subjected to exhaustive attack is overcome, the real-time dynamic coding mechanism is realized, and the attack resistance of image encryption is improved.

The invention discloses an image encryption method based on phase-shift digital holography and a multi-mode biological characteristic key, which comprises the following encryption steps of: firstly, encrypting a grayscale image into a DNA compilation result by using DNA coding and an iris chaos mask; secondly, encrypting a DNA compiling result into three ciphertext holograms by using phase-shift digital holography and fingerprint and finger vein chaos masks; the decryption step comprises the following steps of: firstly, authenticating three biological characteristics of decrypted iris, fingerprint and finger vein; if the authentication is successful, the iris chaos mask and the fingerprint and finger vein conjugate chaos mask for subsequent decryption are generated by the system. And finally, obtaining a final decrypted image by using a digital holographic reconstruction technology and a DNA decoding technology. The method provided by the invention has the advantages of high security level, high biological key robustness and high digital key sensitivity.

The preparation of a library of bispecific binders expressed in eukaryotic cells described herein is a method for producing a cloned library of eukaryotic cells containing DNA encoding a diverse library of bispecific binders, the present invention relates to bispecific conjugates, in particular bispecific conjugates comprising a first binding domain coupled to a first Fc domain and a second binding domain coupled to a second Fc domain.

This invention discloses an attack tracing method and system for end-to-end data transmission in optical communication, belonging to the field of optical fiber communication technology. The method includes: processing an initial key value using a hash function and DNA encoding to generate a DNA sequence; concatenating the DNA sequence to generate a session key and calculating its hash value; calculating a single-byte XOR checksum of the original data to generate extended data, generating ciphertext based on the extended data, and calculating a digest of the ciphertext; decrypting the ciphertext and performing XOR checksum consistency verification to obtain an event digest; calculating a root hash based on the key digest, parameter digest, ciphertext digest, and event digest, storing the root hash in the block header, and performing attack tracing on the block header to obtain the attack tracing result. This invention introduces four-dimensional chaos and DNA encoding to generate a high-entropy key, dynamically storing and updating the key through blockchain, while simultaneously achieving single-root verifiable attack tracing and quickly locating problematic nodes.

Provided herein is a protein from Tumebacillus sp. NITE BP-02779 having a peptidoglycan-decomposing activity. Provided herein is a protein comprising the amino acid sequence of SEQ ID NO: 2 (1-164) or the amino acid sequence of SEQ ID NO: 4 (1-493), or a protein comprising an amino acid sequence in which 1 to 10 amino acid residues of these amino acid sequences are substituted, deleted, inserted, or added, or an amino acid sequence having 90% or more identity to these amino acid sequences, and having a peptidoglycan-decomposing activity.

Synthetic bacterial messenger RNA can be used to make autologous, allogeneic or direct nucleic acid cancer vaccines. Cancer cells are transfected in vitro or in vivo with mRNA obtained from DNA encoding immunogenic bacterial proteins. The immune response to cancer is generated by direct administration of the mRNA in vivo or administration of a vaccine made from cancer cells in vitro. Codon modification of the mRNA can optimize expression of the immunogenic polypeptide in cancer cells.

The present invention relates to a DcR3 variant, DNA encoding the DcR3 variant, a vector containing the DNA, a transformant obtained by introducing the vector, a method for producing a variant using the transformant, and a prophylactic or therapeutic agent for an autoimmune disease, an inflammatory disease or an allergic disease comprising the variant as an active ingredient. The present invention relates to a DcR3 variant having binding activity (preferably neutralizing activity) against a ligand of DcR3 while showing reduced production of aggregates and / or improved in vivo kinetics compared to wild-type DcR3 when prepared with a mammalian-derived cell as a host, and provides a DcR3 variant comprising a portion of DcR3 and a portion of a TNF superfamily molecule.

The present invention relates to a phage-based gene delivery system targeting human cells or animal cells, including immune cells, other human cells and cancer cells. In particular, the present invention relates to novel phage particles and associated phagemid expression systems and their production for the delivery of transgenes, comprising DNA encoding pathogen antigens, to human immune cells, suitable for use in vaccines and vaccine development against infectious diseases and cancers.

The present invention relates to the field of biotechnology and proposes antibodies that bind specifically to PD-L1. Furthermore, the discovery relates to the DNA encoding these antibodies, the corresponding expression vectors and production methods, as well as treatment methods that utilize these antibodies.

The purpose of the present invention is to provide a means for producing a non-animal-derived modified heparosan compound with higher efficiency than conventional means. Provided is any one of the following proteins [1] to [3] fused to a TRX tag. [1] A protein which comprises the amino acid sequence represented by SEQ ID NO: 1. [2] A protein which comprises an amino acid sequence having a structure wherein 1 to 20 amino acid residues are deleted, substituted, inserted, or added in the amino acid sequence represented by SEQ ID NO: 1 and which has N-sulfotransferase activity. [3] A protein which comprises an amino acid sequence having 70% or more identity to the amino acid sequence represented by SEQ ID NO: 1 and has N-sulfotransferase activity.

The application relates to the technical field of communication, in particular to a chaotic image encryption method based on global dynamic selection, which has the technical scheme as follows: S1, generating a chaotic sequence used in the encryption method by using a 2D-LSM chaotic system; S2, expanding an image into a one-dimensional array through raster scanning; S3, dynamically DNA encoding the image to convert the one-dimensional array into a DNA matrix; S4, dynamically permuting the DNA matrix to obtain a permutation image; S5, DNA decoding the permutation image to obtain a DNA decoding matrix; S6, dynamically diffusing and encrypting the DNA decoding matrix to obtain an encrypted image; and S7, repeating steps S2-S6 to repeatedly encrypt the encrypted image as an initial image. The method has the advantages of high flexibility and selectivity.

The present invention provides compounds and methods targeting insulin-like peptide-5 (INSL5), including antibodies, cells and vectors comprising DNA encoding the same, and methods for producing the antibodies. In addition, the present invention provides for use of INSL5 antibodies in diagnostic assays.

The invention discloses a DNA encoding method and device and a DNA decoding method and device, and belongs to the technical field of information storage and security. The DNA encoding method comprises the steps that target information is converted into computer codes; converting the computer code into a DNA coding chain or a DNA coding chain combination; and cross-linking the DNA coding chain and / or the DNA coding chain combination in a storage micro-pool on a DNA information storage medium so as to realize the storage of the target information. According to the method, the information storage process is completely independent of DNA synthesis and DNA sequencing technologies, and the method has the advantages of high parallelism, short access time, good encryption, low cost, strong expansibility and the like.

The application relates to the technical field of DNA encryption, and discloses a DNA encryption method and device based on random combination code tables, equipment and a storage medium, the method comprising the following steps: designing seven code tables with different sizes and a to-be-encoded file; generating a random number according to the file type of the to-be-encoded file; selecting a code table in each code table based on the random number, taking the selected code table as a target code table, then performing encoding conversion according to the target code table and the to-be-encoded file to obtain a DNA encoding result, and generating a random sequence by using chaotic mapping based on the length corresponding to the DNA encoding result; and adjusting the base sequence in the DNA encoding result according to the random sequence to obtain a randomized DNA encryption sequence. The to-be-encoded file can be encoded and converted by using the code table composed of the screened bases, so that the DNA encryption sequence is obtained, the DNA encryption of the to-be-encoded file is realized, and the security and efficiency of encryption are improved.

The invention relates to the technical field of DNA data storage, in particular to a DNA information encryption and error correction method based on secondary structure fingerprint separation storage, which comprises an information DNA encoding stage, a structure signature generation stage, a signature DNA encoding stage, an information DNA and signature DNA synthesis stage and a decoding and error correction stage. The information DNA encoding stage is used for encoding original data into information DNA fragments; the structure signature generation stage is used for calculating structure characteristic parameters, generating a compact structure signature, and associating the fragment ID with the compact structure signature to form a signature data set; the signature DNA encoding stage is used for encoding the signature data set into signature DNA fragments; the information DNA and signature DNA synthesis stage is used for respectively synthesizing an information DNA fragment and a signature DNA fragment, and physically separating and storing the information DNA fragment and the signature DNA fragment; and the decoding and error correction stage is used for verifying and correcting the consensus sequence based on the signature data set to recover the original data. According to the method, the structure signature is stored in the independent DNA molecule, so that physical layer encryption protection is realized.

The present invention relates to a method for producing a microorganism having N-deacetylation activity and N-sulfation activity, the method comprising a step (I) of modifying the nucleotide sequence of DNA encoding an N-deacetylase / N-sulfotransferase, and a step (II) of introducing DNA containing the nucleotide sequence modified in step (I) into the microorganism in an expressible manner, wherein the step (I) is a step of codon optimization according to the frequency of use of codons of a biological species different from the microorganism. Furthermore, the present invention relates to a method for producing a microorganism having N-deacetylation activity and N-sulfation activity, the method comprising the step (i) of expressing a specific protein in the microorganism.

The invention provides an image DNA (deoxyribonucleic acid) storage method for improving information density and biological stability, which comprises the following steps of: partitioning an input image, performing two-dimensional discrete wavelet transform, performing quantization processing on a wavelet coefficient by adopting a self-adaptive quantization strategy, and converting the wavelet coefficient into a binary sequence to be coded; the method comprises the following steps: dividing a binary sequence to be coded into a byte according to every 8 bits, and carrying out dual-mode DNA coding by adopting an odd-even alternating mode and a segmentation structure of'feature segment-coding segment 'to generate a DNA information segment sequence; performing error correction on the coded DNA sequence based on preliminary correction of Hamming distance and RS code secondary error correction; marking the DNA sequence which still does not pass the CRC verification after multiple rounds of error correction as an unrepairable sequence, wherein the image block corresponding to the unrepairable sequence is an error block; and in combination with context information, the neighborhood features of the error blocks are learned by using a cross Transform context repair network, and the error blocks are repaired. According to the method, better balance is achieved in the aspects of image reconstruction quality, coding density and biological compatibility.

The present invention relates to RNA-based methods for inhibiting the expression of the superoxide dismutase 1 (SOD-1) gene. Recombinant adeno-associated viruses of the invention deliver DNAs encoding RNAs that neutralize the expression of SOD-1. The methods have application in the treatment of amyotrophic lateral sclerosis.

A vector construct is described that is a lentiviral construct including DNA encoding for GM-CSF. A vaccine composition is also described that is includes K562 cells transfected with this vector construct, and also possibly including the U266 and H929. Methods are described for using the vaccine composition in methods of immunizing against plasma cell disorders, including multiple myeloma and related disorders.

To provide a novel method that forms a complex linking a peptide with DNA encoding the peptide to establish correspondence therebetween, and to provide a method for readily selecting DNA encoding a desired peptide from a complex library in which peptides correspond to DNA encoding the peptides.SOLUTION: A method for creating a complex in which a peptide is linked to a nucleotide sequence encoding the peptide, the method comprising: (1) preparing circular DNA having a nucleotide sequence encoding a peptide; and (2) transcribing and translating the nucleotide sequence from the circular DNA using a cell-free transcription-translation reaction to express a peptide, wherein the expressed peptide forms a complex linked to the circular DNA.SELECTED DRAWING: None

The application discloses a kind of based on DNA molecule traffic's nanopore real-time high-throughput sequencing system and method, including DNA encoding module, molecular hopper loads DNA sequence chain module, nanopore sequencing module and DNA decoding module;The transmission data required by user is stored in DNA sequence by base pairing;The DNA sequence obtained by encoding is linked with molecular hopper, is transported on the track of nanopore protein, and molecular motor of DNA chain transmission is transmitted on track;Real-time sequencing is completed by the different base pair blocking current different ability;By DNA decoding module, redundancy error caused by molecular hopper in the track of nanopore protein is eliminated.The system provided by the application solves the repeated data deletion error LT real-time error correction algorithm, to eliminate the redundant information generated by hopper bounce back;Eliminate the redundancy caused by hopper back, so as to reduce the high communication cost brought by this kind of error code, obtain greater signal-to-noise ratio, facilitate the application of DNA molecular communication to be popularized.

Synthetic bacterial messenger RNA can be used to make autologous, allogeneic or direct nucleic acid cancer vaccines. Cancer cells are transfected in vitro or in vivo with mRNA obtained from DNA encoding immunogenic bacterial proteins. The immune response to cancer is generated by direct administration of the mRNA in vivo or administration of a vaccine made from cancer cells in vitro. Codon modification of the mRNA can optimize expression of the immunogenic polypeptide in cancer cells.

The present invention relates to method for producing and purifying RNA comprising the steps of providing DNA encoding the RNA; transcription of the DNA into RNA; and conditioning and / or purifying of the solution comprising transcribed RNA by one or more steps of tangential flow filtration (TFF).

An RNA interference-based product and method for inhibiting the expression of the double homeobox 4 (DUX4) gene on human chromosome 4q35 are disclosed. The invention comprises the silencing of the Cas13 protein of RNA, wherein Cas13 is specifically targeted to a DUX4 region of interest using a sequence-specific guide RNA (gRNA). The recombinant adeno-associated virus of the invention is constructed with a Cas13 direct repeat sequence and delivers DNA encoding an inhibitory gRNA that knocks down DUX4 expression. The method is applicable to the treatment of muscular dystrophy, including but not limited to facial-scapulohumeral muscle dystrophy (FSHD), and other disorders, including cancer, associated with elevated DUX4 expression.

The invention provides a method for constructing a DNA (Deoxyribonucleic Acid) encoding library, which specifically comprises the following steps: (s1) providing an HP (High Performance) module which comprises nucleic acid; (s2) covalently linking a chemical structural unit to the HP module to form a chemical moiety linked to the HP module while covalently linking an oligonucleotide encoding the chemical structural unit to the HP module to form an encoding nucleic acid moiety of the HP module to form a nucleic acid-compound conjugate; (s3) carrying out ligation reaction on the nucleic acid-compound conjugate and a compound as shown in a formula (II) to obtain a first mixture; s-R5 (II), in the formula, S is a solid-phase carrier; r5 is a second group participating in the ligation reaction; (s4) filtering the first mixture, washing impurities, purifying, and cutting to obtain a new compound; and (s5) the steps (s2)-(s4) are repeated for x times, and x is an integer greater than 0, so that the DNA coding library is formed. The DNA encoding library has the advantages of higher loading capacity, high purity, longer polypeptide library and wider applicable reaction range.

The invention discloses an attack tracing method and system for optical communication end-to-end data transmission, and belongs to the technical field of optical fiber communication. The method comprises the following steps of: processing a key initial value through a hash function and DNA (Deoxyribose Nucleic Acid) coding to generate a DNA sequence; splicing the DNA sequence to generate a session key and calculating a hash value of the session key; calculating a single-byte XOR check code of the original data to generate extended data, generating a ciphertext according to the extended data, and calculating an abstract of the ciphertext; decrypting the ciphertext and carrying out XOR check code consistency confirmation to obtain an event abstract; and according to the key abstract, the parameter abstract, the ciphertext abstract and the event abstract, calculating root hash, storing the root hash to a block head, and performing attack tracing on the block head to obtain an attack tracing result. According to the method, four-dimensional chaos and DNA coding are introduced to generate a high-entropy key, the key is dynamically stored and updated through the block chain, and meanwhile, single-root verifiable attack traceability is realized, and problem nodes are quickly positioned.

The present invention provides an antigenic peptide to which multiple KRAS mutant peptides are linked, mRNA encoding the same, DNA encoding the same, an immunogenic composition comprising the antigenic peptide to which multiple KRAS mutant peptides are linked or mRNA encoding the same, and a method for inducing an immune response against the KRAS mutant peptide in an individual.

This invention discloses a method for identifying lithium battery equivalent circuit parameters based on a multi-population particle swarm genetic algorithm using DNA encoding: (1) establishing a lithium battery equivalent circuit model with a well-defined structure; (2) obtaining dynamic current and voltage data of the lithium battery under different charging states using hybrid pulse power characteristic testing; (3) optimizing the parameter identification process in the lithium battery equivalent circuit model using a multi-population particle swarm genetic algorithm to obtain the optimal identification parameters; wherein, in step (3), the lithium battery equivalent circuit model obtains the estimated value of the battery terminal voltage at each dynamic moment by substituting the current parameter value and the dynamic current data at each moment in step (2); the fitness function in the multi-population particle swarm genetic algorithm is established based on the estimated value of the battery terminal voltage and the dynamic voltage data / measured voltage at the corresponding moment in step (2). This method has excellent identification effect on lithium battery equivalent circuit parameters.