29 results about "Carrier protein" patented technology

The present invention provides methods, compositions, systems, and kits comprising nano-satellite complexes and / or serum albumin carrier complexes, which are used for modulating antigen-specific immune response (e.g., enhancing anti-tumor immunity). In certain embodiments, the nano-satellite complexes comprise: a) a core nanoparticle complex comprising a biocompatible coating surrounding a nanoparticle core; b) at least one satellite particle attached to, or absorbed to, the biocompatible coating; and c) an antigenic component conjugated to, or absorbed to, the at least one satellite particle component. In certain embodiments, the complexes further comprise: d) a type I interferon agonist agent. In some embodiments, the serum albumin complexes comprise: a) at least part of a serum albumin protein, b) an antigenic component conjugated to the carrier protein, and c) a type I interferon agonist agent.

This invention relates to a medemididine hapten, a complete antigen, and their preparation methods and applications. The structure of the medemididine hapten is shown in Formula I. The preparation method of the medemididine hapten includes: (1) substituting medemididine with ethyl 3-bromopropionate to obtain intermediate 2; (2) adding sodium hydroxide to deprotect the ester group to obtain medemididine hapten 3. The structure of the complete antigen is shown in Formula II. The preparation method of the complete antigen includes: coupling the medemididine hapten with a carrier protein to obtain the complete antigen. This invention discloses a method for preparing medemididine hapten and complete antigen. The synthesis steps are simple and effective, and it can be used in immunoassay. Among them, colloidal gold immunochromatography is simple to operate, has a short detection time, high specificity, and good reproducibility, which can meet the domestic demand for immunoassay of medemididine.

The application provides a benzopyrene immunoaffinity column and a preparation method and application thereof, and a filler of the benzopyrene immunoaffinity column comprises a solid carrier coupled with a benzopyrene monoclonal antibody. The benzopyrene monoclonal antibody in the benzopyrene immunoaffinity column is prepared by using a benzopyrene hapten-carrier protein conjugate as an immunogen, so that the antibody can specifically adsorb benzopyrene substances in petroleum chemicals, effectively improves the recovery rate of benzopyrene, reduces the interference of other impurities such as lipids, proteins and pigments in the petroleum chemicals, and improves the accuracy of benzopyrene detection.

The application discloses a method for preparing a reproductive vaccine based on a novel carrier protein coupled GnRH polypeptide and a product, wherein the amino acid sequence of the carrier protein is shown as SEQ ID NO.1, the gene sequence for coding the carrier protein is shown as SEQ ID NO.2, the gene for coding the carrier protein is subjected to prokaryotic expression, and then subjected to affinity chromatography, anion exchange chromatography purification, TEV enzyme cutting to remove a tag protein, and then subjected to affinity chromatography and molecular sieve to obtain the purified carrier protein, and finally covalently coupled with a GnRH polypeptide to obtain the reproductive vaccine, wherein the reproductive vaccine prepared by the application has a contraceptive effect, and high-quality, low-cost recombinant protein vaccines can be obtained on a large scale for commercialization.

This invention discloses a test strip for detecting thiophanate-methyl and its application. The test strip includes a sample absorption pad (1), a conjugate release pad (2), a reaction membrane (3), an absorbent pad (4), and a base plate (7). The reaction membrane has a detection line (5) coated with a thiophanate-methyl hapten-carrier protein conjugate and a control line (6) coated with goat anti-mouse anti-antibody. The conjugate release pad (2) is coated with a thiophanate-methyl monoclonal antibody-colloidal gold label. This invention also provides a method for detecting thiophanate-methyl residues in vegetables and fruits using the above-mentioned thiophanate-methyl test strip. The test strip provided by this invention has the characteristics of simple operation, high sensitivity, fast detection speed, and low cost, and is suitable for screening large numbers of samples and on-site monitoring.

This invention relates to the field of biochemical technology, and particularly to highly specific haptens of polymyxin B and colistin, artificial antigens, their preparation methods, and applications. The haptens have structures as shown in Formula I or Formula II. The applications include: (1) preparing specific antibodies against polymyxin B or colistin; (2) detecting specific antibodies against polymyxin B or colistin; and (3) preparing reagents for detecting specific antibodies against polymyxin B or colistin. This invention further provides artificial antigens obtained by conjugating the haptens with carrier proteins. Using the artificial antigens provided by this invention as immunogens to immunize experimental animals, highly specific and sensitive antiserum can be prepared, which can then be used to extract and prepare polyclonal antibodies. These methods can be used to establish highly specific, highly sensitive, simple, and rapid detection methods for polymyxin B and colistin, and have significant application value.

This invention belongs to the field of immunoassay technology, specifically relating to acetylpyridinium hapten, complete antigen, antibody, preparation methods, and applications. The acetylpyridinium hapten provided by this invention has the structure shown in Formula I, where R1 is an active group capable of direct coupling with a carrier protein. The acetylpyridinium hapten provided by this invention retains the basic structure of acetylpyridinium, using the original -CH2-CH2-CH2-CH2- structure of acetylpyridinium as a linker arm. An active group R1 capable of direct coupling with a carrier protein is introduced into the linker arm at a site far from the characteristic structure of acetylpyridinium, effectively avoiding non-specific binding caused by the external linker arm. After coupling with the carrier protein, the introduced active group can fully expose the characteristic structure of the acetylpyridinium bulk molecule. The prepared acetylpyridinium complete antigen is immunogenic, and the prepared anti-acetylpyridinium antibody has good specificity and high sensitivity. It can be used to establish immunological detection methods or reagents for acetylpyridinium, and has broad application prospects.

This invention discloses a hapten, an artificial antigen, and their preparation method and application for cycloalkanoic acid markers, relating to the field of artificial antigen technology. A hapten for a cycloalkanoic acid marker is a compound represented by Formula I in Figure 1; an artificial antigen for a cycloalkanoic acid marker is a complete antigen prepared by conjugating the hapten of the compound represented by Formula I with a carrier protein. The core of this invention lies in introducing a specific molecular arm onto the cyclopentylpropionic acid molecule to construct a structurally optimized hapten, which is then conjugated with a carrier protein to form an artificial antigen. It is particularly important to note that the prepared artificial antigen is essentially a polypeptide, capable of effectively stimulating the body's immune response and producing antibodies with high affinity and high specificity.

The application provides an antigen epitope peptide and a paralichthys olivaceus mucin Muc13 specific antibody prepared by the antigen epitope peptide, determines a B cell epitope from the paralichthys olivaceus Muc13 protein through antigen epitope screening, and the sequence is PGTTEEPDPTAGSPP. After the epitope is coupled with a KLH carrier protein, the epitope is used for immunizing New Zealand white rabbits, and a rabbit-derived anti-paralichthys olivaceus Muc13 specific antibody is successfully obtained. The antibody can specifically recognize the Muc13 protein in paralichthys olivaceus mucosa tissue and Muc13 positive mucous cells, and provides a key detection means for in-depth exploration of the function mechanism of mucous cells in the fish mucosa immune response process.

The application discloses a preparation method and application of a cocktail vaccine of Acinetobacter baumannii, and belongs to the technical field of vaccine preparation. The vaccine comprises sugar conjugates formed by coupling three branched pentasaccharides of K14, K47 and K128 with diphtheria toxoid. The preparation method comprises the steps of hapten synthesis, purification, coupling with carrier protein and mixing. Experiments show that the vaccine can induce high levels of specific antibodies, significantly reduce the tissue bacterial load of mice infected with Acinetobacter baumannii, and has a wide protective effect. The application is suitable for preventing and treating various infectious diseases caused by Acinetobacter baumannii.

The application provides an antigen epitope polypeptide and a paralichthys ovatus mucin Muc5b specific antibody prepared by the antigen epitope polypeptide, a B cell antigen epitope of the paralichthys ovatus Muc5b protein is screened, the amino acid sequence of the B cell antigen epitope is shown as NDKQRSKQCEDYQVV, a New Zealand white rabbit is immunized after the antigen epitope is coupled with a KLH carrier protein, and a rabbit anti-paralichthys ovatus Muc5b specific antibody is prepared. The antibody can specifically recognize the Muc5b protein in paralichthys ovatus mucosa tissue and positive mucous cells, and provides a key detection tool for in-depth exploration of the function mechanism of mucous cells in the paralichthys ovatus mucosa immune response process.

The application provides an antigen epitope polypeptide and a paralichthys ovatus mucin Muc1 specific antibody prepared by the antigen epitope, a B cell epitope from the paralichthys ovatus Muc1 protein is identified through antigen epitope screening, and the amino acid sequence is SEQ ID NO:1. After the epitope is coupled with a KLH carrier protein, a New Zealand white rabbit is immunized, and a rabbit anti-paralichthys ovatus Muc1 polyclonal antibody is successfully prepared. The antibody can specifically recognize the Muc1 protein in paralichthys ovatus mucosa tissue and positive mucous cells, and provides an important detection tool for studying the function mechanism of mucous cells in fish mucosal immunity.

This invention discloses a stability-improved complete thyroid hormone antigen, its preparation, and its application. The free amino group of the thyroid hormone or its deiodinated derivative moiety of this complete antigen is protected by a tert-butoxycarbonyl (Boc) group, and its carboxyl group is coupled to a carrier protein via an amide bond. The preparation method includes: protecting the amino group of a thyroid hormone molecule (such as T4, T3, etc.) with Boc2O, followed by activating its carboxyl group using the EDC / NHS method and coupling it to a carrier protein. This invention effectively overcomes the chemical instability problems of traditional complete antigens, such as easy photo-oxidative deiodination, easy racemization, and easy deamination reactions. In vitro detection reagents coated with the complete antigen of this invention exhibit longer shelf life, lower batch-to-batch variability, and extremely stable detection performance.

The present application relates to the field of cell agriculture and animal cell in vitro culture technology, and particularly relates to a Haematococcus pluvialis lysate and a preparation method and application thereof. Through mild wall breaking, controllable enzymolysis, clarification and impurity removal, two-stage tangential flow ultrafiltration molecular weight fractionation and other means, that is, separation is sequentially performed by using 10 kDa and 3 kDa membranes, and combined with stable delivery of lipid-soluble components and standardized compounding, an animal source-free culture supplement which can replace fetal bovine serum is obtained. The supplement can effectively promote fish cell proliferation and realize adipogenic differentiation, realize the integration of amplification and adipogenic compatibility in low serum / serum-free culture of cell cultured fish meat, and significantly reduce batch differences. The technical route can provide adhesion and proliferation support of serum in the proliferation stage, and play the roles of antioxidant, carrier protein-like buffer and lipid metabolism support in the adipogenic stage, and through a clear fractionation process and quality control index, quantifiable, predictable and repeatable production is realized.

The present invention provides a method of producing a polysaccharide-protein conjugate with capsular polysaccharide from Streptococcus pneumoniae serotype 19F conjugated to a carrier protein. The method includes a prolonged incubation step prior to filtration to remove free polysaccharide.

The present application belongs to the technical field of polycyclic aromatic hydrocarbon artificial antigen synthesis, and relates to a novel complete antigen synthesis method for detecting polycyclic aromatic hydrocarbon (PAHs), benzanthracene. The present application successfully prepares a hapten through a Friedel-Crafts acylation reaction, and prepares a complete antigen by coupling the hapten esterized hapten with a carrier protein. The complete antigens have been identified. The present application provides a novel hapten screening, preparation and efficient artificial antigen preparation method for the immunological analysis method of benzanthracene.

An immunomodulatory nanoparticle vaccine carrier protein and its application. This invention uses duck hepatitis B virus core antigen (DHBc) as a base, fuses and expresses the SpyCatcher003 sequence to construct DHBc-SpyCatcher (DHBc-SC) virus-like particles with self-assembly capability, and efficiently expresses them in *E. coli*. When DHBc-SC virus-like particles are mixed with heterologous monomeric antigens containing SpyTag, they can spontaneously couple to form virus-like particles displaying the heterologous antigen, significantly enhancing the immunogenicity of the heterologous antigen.

The application discloses a preparation method and application of an aflatoxin M1 hapten and a whole antigen, wherein the hapten contains two complete active carboxyl groups and can be firmly combined with more carrier proteins. The aflatoxin M1 hapten is obtained by addition of aflatoxin M1 and dimercaptosuccinic acid under catalysis of boron trifluoride ether; the whole antigen is a covalent combination of the hapten and BSA, is prepared by using an EDC / NHS carboxyl coupling method, and can be used for antibody preparation and AFM1 detection.

The present disclosure relates generally to improved methods of preparing glycoconjugates. The methods comprise using a reducing mixture containing acetoxyborohydrides prepared in-situ for conjugating a saccharide to a carrier protein.

The application belongs to the technical field of biological pigment reconstruction and protein engineering, and particularly relates to a pigment complex based on recombinant macrobrachium rosenbergii lipid carrier protein as well as an in-vitro preparation method and application thereof. The formation of the pigment complex relies on the synergistic stabilization of a key residue network of the recombinant macrobrachium rosenbergii lipid carrier protein, a pigment characteristic end group and phosphatidyl ethanolamine. The application has important scientific significance and industrial value for revealing the protein-pigment-lipid ternary interaction rule, constructing a thermal response color-changing system, expanding the application of protein-based pigment materials and providing a technical basis for color biomimetic construction in food and other systems.

This invention discloses a method for preparing aflatoxin M1 hapten and full antigen, and their applications. The hapten contains two intact active carboxyl groups, which can firmly bind to more carrier proteins. The aflatoxin M1 hapten is obtained by the addition reaction of aflatoxin M1 with dimercaptosuccinic acid under the catalysis of boron trifluoride diethyl ether. The full antigen is a covalent conjugate of the hapten and BSA, prepared by the EDC / NHS activated carboxyl coupling method, and can be used for antibody preparation and AFM1 detection.

The application discloses a fentanyl artificial hapten, a fentanyl artificial antigen and a preparation method and application thereof. The fentanyl artificial hapten of the application retains the characteristic structure of fentanyl to the maximum extent, and has an active group which can be coupled with a carrier protein and can serve as an antigenic determinant; the fentanyl artificial antigen prepared further can be used for immunization to obtain an anti-fentanyl antibody with high affinity, high sensitivity and high specificity, and the titer of the immune serum obtained by immunizing a New Zealand white rabbit is as high as 1:85000. The antigen and antibody can be used for enzyme-linked immunoassay, immunochromatography and the like, have the characteristics of high sensitivity, high specificity, strong operability and the like, and can be used for screening and detection of fentanyl components in various complex samples.

Provided is a nucleic acid delivery system for achieving the delivery of target nucleic acids, wherein the structure of the nucleic acid delivery system includes: a self-packaging carrier protein and target nucleic acids. The self-packaging carrier protein includes: a self-assembly region and a membrane-promoting region, wherein the self-packaging carrier protein and the target nucleic acids are combined into a whole through a nucleic acid binding region, and the target nucleic acids can be expressed after being delivered to the specific cells through the nucleic acid delivery system.

This invention discloses a pyrifensulfuron hapten, an artificial antigen, an antibody, and their preparation methods and applications. The structure of the pyrifensulfuron hapten is shown in Formula (I). This invention obtains a hapten by introducing a carboxyl active group with a certain length of spacer arm onto the benzene ring of pyrifensulfuron, which can then be coupled with a carrier protein to obtain an artificial antigen for immunization. This pyrifensulfuron hapten retains all the characteristic groups of pyrifensulfuron, with minimal alteration to the original structural features of pyrifensulfuron, laying the foundation for subsequent stimulation of animal immune responses to produce antibodies with higher specificity and sensitivity. The artificial antigen and antibody are then prepared using the above hapten. The resulting pyrifensulfuron monoclonal antibody, based on an indirect competitive ELISA using the pyrifensulfuron monoclonal antibody, shows a limit of detection of 2.30 ng / mL for pyrifensulfuron, with an IC50 value of [missing value]. 50 The effective concentration was 17.65 ng / mL, with a linear range of 4.87–63.94 ng / mL, indicating high detection sensitivity and a wide linear range.

Provided is a GnRH nucleic acid vaccine. GnRH is linked by means of a specific linker arm to form a multimer, or the GnRH or multimer is linked to a carrier protein to form a fusion antigen. Compared with a conventional linker arm, the expression of the multimer or fusion antigen via mRNA achieves higher expression levels and provides better presentation of GnRH epitopes; and an mRNA vaccine developed by encapsulating mRNA in liposomes can be used for castrating animals or pets, thereby effectively controlling the fertility of animals or pets.

The present application relates to a kind of nanoparticles, nano drop eye drops and its preparation method and application, belong to medical technology field.The present application provides a kind of nanoparticle, the nanoparticle is composed of tyrosine kinase inhibitor and carrier protein;The mass ratio of tyrosine kinase inhibitor and carrier protein is 2~4:1.The present application prepares a kind of nanoparticle loaded tyrosine kinase inhibitor, creatively combines it with light-sensitive agent loaded lipid vesicle, obtains a kind of synergistic photodynamic-VEGF inhibits bifunctional light-responsive nano drop eye drops, its particle size is uniform, preparation method is simple and easy to operate, and stability is good, realizes the efficient co-delivery of light-sensitive agent, tyrosine kinase inhibitor, with laser response release behavior, and can be long time in eye tissue Retention, not only greatly improve the bioavailability of hydrophobic drug, and realize the final curative effect of anti-ocular angiogenesis, with potential clinical application value.