31 results about "Sequence selectivity" patented technology

A method for the selective amplification of a target sequence. The method includes hybridizing a tagged oligonucleotide to the target sequence and reducing the effective concentration of unhybridized tagged oligonucleotide which is capable of hybridizing to the target sequence. The tagged oligonucleotide includes a target hybridizing sequence and a tag sequence situated 5′ to target hybridizing sequence, where the tag sequence does not stably hybridize to a target nucleic acid containing the target sequence. A blocker oligonucleotide is provided which is designed to hybridize to a region of a nucleic acid containing the target sequence, where the region targeted by the blocker oligonucleotide is 3′ to the target sequence. Amplification products are formed using first and second oligonucleotides. The first oligonucleotide hybridizes to the 3′-end of the complement of the target sequence, and the second oligonucleotide hybridizes to the complement of the tag sequence.

An enzyme composite and a method for regulating nuclease sequence selectivity are disclosed. A novel chemical regulation means is developed, nucleases (DNase and RNase) which don't have sequence specificity originally are allowed to obtain sequence selectivity, and the sequence selectivity of the enzymes can be regulated flexibly according to requirements of a needed cleavage sequence. Accordingly, a new tool for molecular biology study, medicine development, development of genetic engineering techniques, and the like is provided.

Machine control parameters of a magnetic resonance apparatus are selected that influence the timing sequence of gradient pulses of the system's gradient system when a magnetic resonance measurement sequence is executed. The machine control parameters are compared with reference control parameters that indicate an increased mechanical force flow in the gradient system when the MR measurement sequence is being executed. As a function of the comparison, the MR measurement sequence is executed selectively with the selected machine control parameters.

In a method and magnetic resonance (MR) apparatus for acquiring MR data from a volume of an object in which first and second excitable spin types are present that differ in their Larmor frequencies by a chemical shift, an MR sequence with at least one radio-frequency pulse sequence selectively excites the first spin type or selectively suppresses MR signals of the second spin type. A B0 map describing the basic field distribution in a region of interest of the volume is established. First and second items of distribution information, which respectively describe the spectral distribution of Larmor frequencies of the first and second spin types, are derived from the B0 map. A pulse sequence parameter that describes the excitation spectrum of the radio-frequency pulse sequence is optimized based on the items of distribution information, with regard to a quality criterion that optimizes selective excitation and / or suppression.

A new polypeptide prepared from human library by the SST method and a process for preparation of it; a cDNA encoding the polypeptide; a fragment selectively hybridizing with the sequence of the cDNA; a replication or expression plasmid containing the cDNA integrated thereinto; a host cell transformed with plasmid; an antibody against the polypeptide; and a pharmaceutical composition containing the polypeptide or the antibody.

There are disclosed compositions and methods to render nucleic acids resistant to nuclease digestion while maintaining sequence selective hybridization competency. The approach relies on utilizing nucleic acids as the polar head group of a nucleic acid-polymer amphiphile in order to assemble well-defined, discrete micellar nanoparticles. Dense packing of nucleic acid in the micelle corona allows for hybridization of complementary oligonucleotides while prohibiting enzymatic degradation.

The present invention relates to supramolecular polymers containing sequence-selective hydrogen bonding subunits in their backbone which form double helices. The invention allows for tuning of the numbers and sequences of donor / acceptor units incorporated in any one crosslinking hydrogen bonding subunit and hence tuning of the interaction strength not only through the amount of crosslinking material incorporated but also through modulation of the strength of the crosslinking interactions. It also allows for the incorporation of more than one type of crosslinking agent in the material allowing for multiple strengths of crosslinking which are each tunable with regard to disruption from solvent, temperature and stress. Hydrogen bond strength between oligomeric chains can be tailored through modification of the numbers and sequences of the donors / acceptors in the oligomers. The oligomers are sequence-specific and will generally only hydrogen-bond to oligomeric chains which are composed of a complementary set of donors / acceptors. The hydrogen bonded motif formed by the interacting oligomers is helical, imparting both chirality and intertwined topology to these interaction points. Because the polymer end units react with their complements through hydrogen bonding, the telechelic polymer(s) incorporating this technology are reversibly able to be processed as the bonds are first broken and then reformed. This has applications in a number of fields such as inkjet inks, adhesives, printing plates and microphase patterning of polymer surfaces.

The invention discloses an artificial nuclease and a preparation method thereof, belonging to the technical field of biochemistry and aiming at providing an efficient artificial nuclease and a preparation method thereof according to a restriction relation among the solubility, molecular structure, size and activity of the traditional nuclease. A water soluble functional metal compound is prepared from copper chloride, glycine and pi-electronic groups in a water phase through one-step chemical reaction, is of a plane or tetragonal pyramidal structure, and has strong insertion and hydrolysis capacity on nucleic acid, and stronger sequence selectivity and connection capacity; and by using a mixed solvent volatilizing method, the crystallizing efficiency of products is quickened, and the purity and the yield are improved. Copper at the center of the compound obtained from the invention is respectively of a three-dimensional tetragonal pyramidal structure and a plane quadrilateral structure, thus the artificial nuclease is endowed with tRNA targeting identification and biological connection genes, and has stronger binding constant to the nucleic acid and high hydrolysis rate.

Embodiments are disclosed for a sequence selective symbol checker for communication systems. An example method includes configuring a symbol checker of a receiver with first binary sequence data generated by a symbol generator of the receiver. The example method also includes comparing, using the symbol checker, second binary sequence data provided by a transmitter to the first binary sequence data to generate error count data related to a number of errors for symbols associated with the second binary sequence data. The example method also includes determining total count data related to a number of symbols associated with the first binary sequence data. The example method also includes determining error ratio data associated with the transmitter based on the error count data and the total count data.

Embodiments are disclosed for a sequence selective symbol checker for communication systems. An example method includes configuring a symbol checker of a receiver with first binary sequence data generated by a symbol generator of the receiver. The example method also includes comparing, using the symbol checker, second binary sequence data provided by a transmitter to the first binary sequence data to generate error count data related to a number of errors for symbols associated with the second binary sequence data. The example method also includes determining total count data related to a number of symbols associated with the first binary sequence data. The example method also includes determining error ratio data associated with the transmitter based on the error count data and the total count data.

Disclosed are an ABA-type triblock copolymer based on a molecular glue, synthesis therefor, and use thereof. The structural formula of the copolymer is selected from the Formula (I). The copolymer of the present invention can be used to prepare a self-assembled medicine-carrying micelle. The present invention is based on the hydrophilicity of polyethylene glycol and chitosan of a low molecular weight, and on the hydrophobicity of polylactic acid, polycaprolactone, lauric acid, and stearate. Single chains of molecular glue having hydrogen bond sequence-selectivity are separately introduced into known macro-molecular polymers, such that under certain conditions, two complimentary single chains can form a molecular glue. A series of amphiphilic triblock copolymers can thereby effectively synthesized. Additionally, the molecular glue introduced contains a disulfide bond, and the copolymer is therefore sensitive to oxidoreduction. The raw material agents for the synthesis of the present invention can be easily obtained, the conditions are temperate, and the synthesis process contains only conventional chemical reactions and is suitable for large-scale production.

The present invention relates to an affinity ligand capable of selective interaction with an epitope sequence consisting of 47 amino acids or less and comprising the amino acid sequence of SEQ ID NO:1 and / or SEQ ID NO:2. Further, it relates to a polypeptide consisting of the epitope sequence and to uses of the affinity ligand and the polypeptide.

Peptide nucleic acids containing thymidine and 2-aminopyridine (M) nucleobases formed stable and sequence selective triple helices with double stranded RNA at physiologically relevant conditions. The M-modified PNA displayed unique RNA selectivity by having two orders of magnitude higher affinity for the double stranded RNAs than for the same DNA sequences. Preliminary results suggested that nucleobase-modified PNA could bind and recognize double helical precursors of microRNAs.

The present invention relates to sequence selective compounds for targeting therapeutic or diagnostic groups to polynucleotides. More particularly, the present invention relates to sequence selective targeting of metallocomplexes, such as metallodrugs and metallodiagnostics, to polynucleotides.

Novel polypeptides produced by a human adult brain tissue, a cell line derived therefrom, a cell line derived from human bone marrow and a human umbilical cord venous endothelial cell line; a process for producing these polypeptides; cDNAs encoding the polypeptides; fragments hybridizable selectively with the cDNA sequences; replication or expression plasmids having the cDNAs integrated thereinto; host cells transformed by the plasmids; antibodies against the above polypeptides; and medicinal compositions containing the peptides or the antibodies.

An enzyme composite and a method for regulating nuclease sequence selectivity are disclosed. A novel chemical regulation means is developed, nucleases (DNase and RNase) which don't have sequence specificity originally are allowed to obtain sequence selectivity, and the sequence selectivity of the enzymes can be regulated flexibly according to requirements of a needed cleavage sequence. Accordingly, a new tool for molecular biology study, medicine development, development of genetic engineering techniques, and the like is provided.

The present invention relates to supramolecular polymers containing sequence-selective hydrogen bonding subunits in their backbone which form double helices. The invention allows for tuning of the numbers and sequences of donor / acceptor units incorporated in any one crosslinking hydrogen bonding subunit and hence tuning of the interaction strength not only through the amount of crosslinking material incorporated but also through modulation of the strength of the crosslinking interactions. It also allows for the incorporation of more than one type of crosslinking agent in the material allowing for multiple strengths of crosslinking which are each tunable with regard to disruption from solvent, temperature and stress. Hydrogen bond strength between oligomeric chains can be tailored through modification of the numbers and sequences of the donors / acceptors in the oligomers. The oligomers are sequence-specific and will generally only hydrogen-bond to oligomeric chains which are composed of a complementary set of donors / acceptors. The hydrogen bonded motif formed by the interacting oligomers is helical, imparting both chirality and intertwined topology to these interaction points. Because the polymer end units react with their complements through hydrogen bonding, the telechelic polymer(s) incorporating this technology are reversibly able to be processed as the bonds are first broken and then reformed. This has applications in a number of fields such as inkjet inks, adhesives, printing plates and microphase patterning of polymer surfaces.

A polypeptide prepared from mouse ES cell strains by the SST method and a homologous polypeptide obtained from mouse kidney, human uterus, mouse fetus, human fetal liver and pancreas libraries; a cDNA encoding the polypeptide; a fragment selectively hybridizing with the sequence of the cDNA; a replication or expression plasmid containing the cDNA integrated thereinto; a host cell transformed with plasmid; an antibody against the polypeptide; and a pharmaceutical composition containing the polypeptide or the antibody.

The present invention refers to a conjugate comprising a nanoparticle and at least one oligonucleotide analog, wherein the at least one oligonucleotide analog is a phosphorodiamidate morpholino oligo (PMO) or a derivative thereof that is covalently coupled to the nanoparticle, methods for detecting a target nucleic acid using the conjugate and a kit comprising the conjugate.

The present invention relates to novel achiral seco-analogues of the DNA minor groove and sequence-selective alkylating agents (+)-CC1065 and the duocarmycins, depicted as general class (I), (II), (III), (IV) and (V) wherein X is a good leaving group, such as chloride, a bromide, an iodide, a mesylate, a tosylate, an acetate, a quaternary ammonium moiety, a mercaptan, an alkylsulfoxyl, or an alkylsulfonyl group, preferably either a chloride, a bromide, or an iodide group. R1 is a suitable minor groove binding agent to enhance the interactions of the achiral seco-cyclopropaneindole (CI) or an achiral seco-duocarmycin with specific sequences of DNA. R and R2-R5 are defined in claim 1.