44 results about "Sequence selectivity" patented technology

A server information handling system integrated management controller selectively communicates management information between a baseboard management controller and either a network remote console session or local I / O devices, such as a display, a keyboard, a mouse or a KVM appliance. Display information is communicated from the baseboard management controller to a display by writing from the baseboard management controller through a unified memory controller to a display frame buffer for presentation at the display. Keyboard, mouse and KVM information is communicated through a USB host to the baseboard management controller or, in an operational state, is communicated from the USB host to a USB client for use by the server information handling system. In an operational state, a break sequence selectively interfaces the I / O devices with the server information handling system or the baseboard management controller.

A method for the selective amplification of a target sequence. The method includes hybridizing a tagged oligonucleotide to the target sequence and reducing the effective concentration of unhybridized tagged oligonucleotide which is capable of hybridizing to the target sequence. The tagged oligonucleotide includes a target hybridizing sequence and a tag sequence situated 5′ to target hybridizing sequence, where the tag sequence does not stably hybridize to a target nucleic acid containing the target sequence. A blocker oligonucleotide is provided which is designed to hybridize to a region of a nucleic acid containing the target sequence, where the region targeted by the blocker oligonucleotide is 3′ to the target sequence. Amplification products are formed using first and second oligonucleotides. The first oligonucleotide hybridizes to the 3′-end of the complement of the target sequence, and the second oligonucleotide hybridizes to the complement of the tag sequence.

The invention discloses a method for improving selective mapping and a device for improving the selective mapping of phase sequences by using superimposed training sequences. The method comprises the following steps of: performing serial / parallel conversion on input signal sequences by a sending terminal and extracting partial parallel signal sequences from the converted signal sequences for execution; adding the parallel signal sequences to the superimposed training sequences in a one-to-one manner in consideration of power distribution; processing the addition result and the remaining parallel signal sequences after the extraction in parallel; multiplying all the parallel signals with an element corresponding to a sequence in an N-cluster phase sequence group and performing Fast Fourier Transform on the multiplied result; computing the corresponding own peak-to-average power ratio (PAPR) by the sending terminal, and selecting the phase sequence as the relatively optimum phase sequence, namely, suboptimum phase sequence once the PAPR is less than or equal to the preset threshold, or selecting the phase sequence corresponding to the minimum PAPR as the suboptimum phase sequence; and finally, using the parallel signal corresponding to the minimum PAPR in the suboptimum phase sequence as the result and sending the result.

The invention discloses an ABA type amphiphilic triblock copolymer based on molecular glue and uses of the same. The copolymer structure is as follows: the copolymer can be used for preparing a self-assembled loading drug micelle. The ABA type amphiphilic triblock copolymer based on molecular glue and uses of the same utilize a method of organic synthesis to modify polymer polyethylene glycol (PEG) and poly lactic acid (PLA) for the first time, introduce a molecular glue single chain with a hydrogen bond sequence selectivity into a polymer macromolecule, and enable the molecular glue single chain to form a molecular glue under certain conditions so as to selectively efficient synthesize the series amphiphilic triblock copolymer. The synthetic raw material of the compound is simple and easy to prepare, each synthesizing process is a conventional reaction, and suitable for mass production. The prepared block copolymer micelle has good stability, a blank micelle does no harm to normal cells, and the drug-loading micelle can effectively release encapsulated drugs under the reductants of dithiothreitol or reductible glutathione.

The present invention refers to a conjugate comprising a nanoparticle and at least one oligonucleotide analog, wherein the at least one oligonucleotide analog is a phosphorodiamidate morpholino oligo (PMO) or a derivative thereof that is covalently coupled to the nanoparticle, methods for detecting a target nucleic acid using the conjugate and a kit comprising the conjugate.

Machine control parameters of a magnetic resonance apparatus are selected that influence the timing sequence of gradient pulses of the system's gradient system when a magnetic resonance measurement sequence is executed. The machine control parameters are compared with reference control parameters that indicate an increased mechanical force flow in the gradient system when the MR measurement sequence is being executed. As a function of the comparison, the MR measurement sequence is executed selectively with the selected machine control parameters.

There are disclosed compositions and methods to render nucleic acids resistant to nuclease digestion while maintaining sequence selective hybridization competency. The approach relies on utilizing nucleic acids as the polar head group of a nucleic acid-polymer amphiphile in order to assemble well-defined, discrete micellar nanoparticles. Dense packing of nucleic acid in the micelle corona allows for hybridization of complementary oligonucleotides while prohibiting enzymatic degradation.

An enzyme composite and a method for regulating nuclease sequence selectivity are disclosed. A novel chemical regulation means is developed, nucleases (DNase and RNase) which don't have sequence specificity originally are allowed to obtain sequence selectivity, and the sequence selectivity of the enzymes can be regulated flexibly according to requirements of a needed cleavage sequence. Accordingly, a new tool for molecular biology study, medicine development, development of genetic engineering techniques, and the like is provided.

Machine control parameters of a magnetic resonance apparatus are selected that influence the timing sequence of gradient pulses of the system's gradient system when a magnetic resonance measurement sequence is executed. The machine control parameters are compared with reference control parameters that indicate an increased mechanical force flow in the gradient system when the MR measurement sequence is being executed. As a function of the comparison, the MR measurement sequence is executed selectively with the selected machine control parameters.

In a method and magnetic resonance (MR) apparatus for acquiring MR data from a volume of an object in which first and second excitable spin types are present that differ in their Larmor frequencies by a chemical shift, an MR sequence with at least one radio-frequency pulse sequence selectively excites the first spin type or selectively suppresses MR signals of the second spin type. A B0 map describing the basic field distribution in a region of interest of the volume is established. First and second items of distribution information, which respectively describe the spectral distribution of Larmor frequencies of the first and second spin types, are derived from the B0 map. A pulse sequence parameter that describes the excitation spectrum of the radio-frequency pulse sequence is optimized based on the items of distribution information, with regard to a quality criterion that optimizes selective excitation and / or suppression.

There are disclosed compositions and methods to render nucleic acids resistant to nuclease digestion while maintaining sequence selective hybridization competency. The approach relies on utilizing nucleic acids as the polar head group of a nucleic acid-polymer amphiphile in order to assemble well-defined, discrete micellar nanoparticles. Dense packing of nucleic acid in the micelle corona allows for hybridization of complementary oligonucleotides while prohibiting enzymatic degradation.

The present invention relates to supramolecular polymers containing sequence-selective hydrogen bonding subunits in their backbone which form double helices. The invention allows for tuning of the numbers and sequences of donor / acceptor units incorporated in any one crosslinking hydrogen bonding subunit and hence tuning of the interaction strength not only through the amount of crosslinking material incorporated but also through modulation of the strength of the crosslinking interactions. It also allows for the incorporation of more than one type of crosslinking agent in the material allowing for multiple strengths of crosslinking which are each tunable with regard to disruption from solvent, temperature and stress. Hydrogen bond strength between oligomeric chains can be tailored through modification of the numbers and sequences of the donors / acceptors in the oligomers. The oligomers are sequence-specific and will generally only hydrogen-bond to oligomeric chains which are composed of a complementary set of donors / acceptors. The hydrogen bonded motif formed by the interacting oligomers is helical, imparting both chirality and intertwined topology to these interaction points. Because the polymer end units react with their complements through hydrogen bonding, the telechelic polymer(s) incorporating this technology are reversibly able to be processed as the bonds are first broken and then reformed. This has applications in a number of fields such as inkjet inks, adhesives, printing plates and microphase patterning of polymer surfaces.

A method for identifying a compound that has a biocidal effect against a selected organism involves screening from among known or unknown peptide or non-peptide molecules, a test molecule that binds selectively to a target sequence of a multi-helical lid of a heat shock protein of the organism. The binding of the test compound inhibits the protein folding activity of the protein. A specific embodiment of such a method is useful for identifying or designing a pharmaceutical or veterinary biocidal or antibiotic compound, preferably a pathogen and / or strain-specific compound. For this purpose, the compound does not bind to a heat shock protein that is homologous to the mammalian subject to be treated with the compound. Screening methods can encompass direct binding or competitive assays. Molecules or compounds identified by these methods are employed as biocides for pharmaceutical, veterinary, pesticide, insecticide and rodenticide uses, among others.

The present invention relates to novel achiral seco-analogues of the DNA minor groove and sequence-selective alkylating agents (+)-CC1065 and the duocarmycins, depicted as general class (I), (II), (III), (IV) and (V) wherein X is a good leaving group, such as chloride, a bromide, an iodide, a mesylate, a tosylate, an acetate, a quaternary ammonium moiety, a mercaptan, an alkylsulfoxyl, or an alkylsulfonyl group, preferably either a chloride, a bromide, or an iodide group. R1 is a suitable minor groove binding agent to enhance the interactions of the achiral seco-cyclopropaneindole (CI) or an achiral seco-duocarmycin with specific sequences of DNA. R and R2-R5 are defined in claim 1.

The present invention relates to the field of biomedicine, and particularly to an assembled protein nanocarrier CABRi for targeted delivery of small interfering nucleic acids, its preparation method and application. The assembled protein nanocarrier CABRi (CABRi series of siRNA protein delivery carriers) designed by the present invention can efficiently load siRNA without sequence selectivity, and can be mixed with one or more siRNAs in a certain ratio to form a carrier / small nucleic acid complex CABRi-siRNA, which self-assembles into a nanoparticle with a size of 10-100 nm. The present invention constructs a brand-new siRNA delivery carrier, breaking through three key technical bottlenecks in siRNA drug delivery, namely, extremely low in vivo delivery efficiency of siRNA, endosomal escape and extrahepatic delivery problems, and finally achieving the purposes of efficient delivery, endosomal escape, targeting extrahepatic organs or tumors, high safety and easy industrialization of siRNA drugs.

Embodiments of the invention provide a method for in-situ selective deposition and etching for advanced patterning applications. According to one embodiment the method includes providing in a process chamber a substrate having a metal-containing layer thereon, and exposing the substrate to a gas pulse sequence to etch the metal-containing layer in the absence of a plasma, where the gas pulse sequence includes, in any order, exposing the substrate to a first reactant gas containing a halogen-containing gas, and exposing the substrate to a second reactant gas containing an aluminum alkyl. According to another embodiment, the substrate has an exposed first material layer and an exposed second material layer, and the exposing to the gas pulse sequence selectively deposits an additional material layer on the exposed first material layer but not on the exposed second material layer.

This invention belongs to the field of compound technology and provides a DNA-intercalating fluorescent quenching compound, its preparation method, and its application. This invention provides a DNA-intercalating fluorescent quenching compound that exhibits absorption in the visible light region and certain DNA sequence selectivity. The DNA-intercalating fluorescent quenching compound provided by this invention can be further used to design fluorescence analysis systems for restriction endonuclease EcoRV and DNases. The DNA-intercalating quenching compound can achieve relevant fluorescence analysis by combining with fluorescent molecules in a covalent-free manner, reducing the need for chemical labeling steps, thereby improving efficiency and reducing detection costs, and showing good application prospects in related analyses.

The invention discloses an artificial nuclease and a preparation method thereof, belonging to the technical field of biochemistry and aiming at providing an efficient artificial nuclease and a preparation method thereof according to a restriction relation among the solubility, molecular structure, size and activity of the traditional nuclease. A water soluble functional metal compound is prepared from copper chloride, glycine and pi-electronic groups in a water phase through one-step chemical reaction, is of a plane or tetragonal pyramidal structure, and has strong insertion and hydrolysis capacity on nucleic acid, and stronger sequence selectivity and connection capacity; and by using a mixed solvent volatilizing method, the crystallizing efficiency of products is quickened, and the purity and the yield are improved. Copper at the center of the compound obtained from the invention is respectively of a three-dimensional tetragonal pyramidal structure and a plane quadrilateral structure, thus the artificial nuclease is endowed with tRNA targeting identification and biological connection genes, and has stronger binding constant to the nucleic acid and high hydrolysis rate.

Embodiments are disclosed for a sequence selective symbol checker for communication systems. An example method includes configuring a symbol checker of a receiver with first binary sequence data generated by a symbol generator of the receiver. The example method also includes comparing, using the symbol checker, second binary sequence data provided by a transmitter to the first binary sequence data to generate error count data related to a number of errors for symbols associated with the second binary sequence data. The example method also includes determining total count data related to a number of symbols associated with the first binary sequence data. The example method also includes determining error ratio data associated with the transmitter based on the error count data and the total count data.

The present invention relates to a method for the multiplexed detection of nucleic acids in a sample. This is accomplished by using novel sets of primer pairs containing tails which can be selectively captured by complementary tail sequences with negligible cross-hybridization. The invention relates also to reagents, kits and devices used to implement the multiplexed detection method.

Embodiments are disclosed for a sequence selective symbol checker for communication systems. An example method includes configuring a symbol checker of a receiver with first binary sequence data generated by a symbol generator of the receiver. The example method also includes comparing, using the symbol checker, second binary sequence data provided by a transmitter to the first binary sequence data to generate error count data related to a number of errors for symbols associated with the second binary sequence data. The example method also includes determining total count data related to a number of symbols associated with the first binary sequence data. The example method also includes determining error ratio data associated with the transmitter based on the error count data and the total count data.

Disclosed are an ABA-type triblock copolymer based on a molecular glue, synthesis therefor, and use thereof. The structural formula of the copolymer is selected from the Formula (I). The copolymer of the present invention can be used to prepare a self-assembled medicine-carrying micelle. The present invention is based on the hydrophilicity of polyethylene glycol and chitosan of a low molecular weight, and on the hydrophobicity of polylactic acid, polycaprolactone, lauric acid, and stearate. Single chains of molecular glue having hydrogen bond sequence-selectivity are separately introduced into known macro-molecular polymers, such that under certain conditions, two complimentary single chains can form a molecular glue. A series of amphiphilic triblock copolymers can thereby effectively synthesized. Additionally, the molecular glue introduced contains a disulfide bond, and the copolymer is therefore sensitive to oxidoreduction. The raw material agents for the synthesis of the present invention can be easily obtained, the conditions are temperate, and the synthesis process contains only conventional chemical reactions and is suitable for large-scale production.

Peptide nucleic acids containing thymidine and 2-aminopyridine (M) nucleobases formed stable and sequence selective triple helices with double stranded RNA at physiologically relevant conditions. The M-modified PNA displayed unique RNA selectivity by having two orders of magnitude higher affinity for the double stranded RNAs than for the same DNA sequences. Preliminary results suggested that nucleobase-modified PNA could bind and recognize double helical precursors of microRNAs.

The present invention relates to an affinity ligand capable of selective interaction with an epitope sequence consisting of 47 amino acids or less and comprising the amino acid sequence of SEQ ID NO:1 and / or SEQ ID NO:2. Further, it relates to a polypeptide consisting of the epitope sequence and to uses of the affinity ligand and the polypeptide.

Peptide nucleic acids containing thymidine and 2-aminopyridine (M) nucleobases formed stable and sequence selective triple helices with double stranded RNA at physiologically relevant conditions. The M-modified PNA displayed unique RNA selectivity by having two orders of magnitude higher affinity for the double stranded RNAs than for the same DNA sequences. Preliminary results suggested that nucleobase-modified PNA could bind and recognize double helical precursors of microRNAs.

Novel polypeptides produced by a human adult brain tissue, a cell line derived therefrom, a cell line derived from human bone marrow and a human umbilical cord venous endothelial cell line; a process for producing these polypeptides; cDNAs encoding the polypeptides; fragments hybridizable selectively with the cDNA sequences; replication or expression plasmids having the cDNAs integrated thereinto; host cells transformed by the plasmids; antibodies against the above polypeptides; and medicinal compositions containing the peptides or the antibodies.