The invention discloses a sunflower disc water extractive with the xanthine oxidase inhibitory activity, a preparation method and application thereof, and belongs to the technical field of medical science. The method comprises the steps that sunflower discs are dried, ground and screened by a 60-mesh sieve to obtain a crude product; 3-8 g of the crude product is added into 100 mL distilled water,oscillation water extraction is performed for 1-3 h under the temperature of 50-100 DEG C, centrifuging is performed under the rate of 3,000-8,000 rpm / min for 10-20 min, and a filtrate I is obtained;precipitates are collected, 100 mL of distilled water is added, oscillation water extraction is performed under the same condition, and centrifuging is performed to obtain a filtrate II; precipitatesare collected, 50 mL of distilled water is added, oscillation water extraction is performed under the same condition, and centrifuging is performed to obtain a filtrate III; the filtrates I, II and III are combined; ultrafiltration is performed on the filtrates, then, the filtrates pass through a filter membrane of the size being 0.45 micrometer, and freeze drying is performed to obtain the sunflower disc water extractive; the yield is 25.00-39.20%. The sunflower disc water extractive can be applied for preparing medicine or food for reducing uric acid and promoting mouse kidney health.