162 results about "Paeoniflorin" patented technology

The invention discloses a planting method for increasing the content of anthocyanin in purple rice grains, which comprises the following specific steps: sowing purple rice seeds from May 20th to May 30th every year, and setting the nitrogen application amount to be 24kg N / mu and the planting density to be 1.58-2.22 million holes / mu at the same time. According to the method, the sowing time, the nitrogen application amount and the planting density are precisely and cooperatively regulated and controlled, resource distribution in the key period of purple rice anthocyanin synthesis is directionally optimized, the content of paeoniflorin and cyanidin in grains is remarkably increased, meanwhile, yield stability and efficient resource utilization are both considered, and through cultivation technology innovation which is low in cost and easy to popularize, the yield of purple rice is increased. The multi-target of directional enhancement of functional components of purple rice, stable yield and high resource efficiency is achieved, a technical normal form is provided for green transformation of functional agriculture, remarkable economic, ecological and social benefits are achieved, and the important requirement for green and high-efficiency development of functional agriculture is met.

The invention discloses a silk fibroin-hyaluronic acid temperature-sensitive bioactive hydrogel scaffold, a preparation method thereof and application of the silk fibroin-hyaluronic acid temperature-sensitive bioactive hydrogel scaffold in TBI treatment, hyaluronic acid, silk fibroin and N-isopropylacrylamide are used as raw materials to construct temperature-sensitive hydrogel, and bone marrow mesenchymal stem cells (BMSCs) and paeoniflorin (PF) are loaded to form a treatment unit. Hyaluronic acid is one of components of an extracellular matrix (ECM), has high affinity to cells, and can promote proliferation of stem cells. Silk fibroin has high biocompatibility and biodegradability and good mechanical strength and ductility / toughness, and can promote tissue repair and stem cell attachment and proliferation. N-isopropylacrylamide endows the hydrogel with the characteristic of in-situ temperature-sensitive gelling. Therefore, the bioactive hydrogel stent has the advantages of components, combines the BMSCs and the PF, overcomes the obstacle that the medicine is limited by BBB to be transferred into the brain, and is implanted into a brain injury part after a TBI surgical operation to be used for repairing and treating nerve injury.

The invention provides an anti-depression composition and a preparation method thereof. The composition is prepared from the following components in parts by mass: 1 part of saikoside D, 2 to 6 parts of saikoside A, 20 to 25 parts of paeoniflorin, 6 to 9 parts of albiflorin, 12 to 15 parts of apiose liquiritin, 2 to 4 parts of apiose isoliquiritin and 4 to 6 parts of atractylenolide I. Compared with western medicine fluoxetine and classic famous formula Xiaoyao powder which are sold on the market under the clinical equivalent dosage condition in the depression cell and depression animal experiment dimension, cell experiments and animal experiments both show that the medicine effect of the anti-depression composition is far higher than the anti-depression effect of the western medicine fluoxetine, and meanwhile, the anti-depression composition has the advantages that the anti-depression effect of the anti-depression composition is greatly improved. The medicine effect is higher than the anti-depression effect of the whole prescription of the Xiaoyao powder

The invention belongs to the technical field of solar cells, and particularly discloses a texturing additive, a texturing solution and a texturing method of a back contact cell. The texturing additive for the back contact battery comprises the following components in percentage by mass: 1.0%-5.0% of sodium carboxymethyl cellulose, 0.03%-0.2% of sodium lignin sulfonate, 0.003%-0.03% of paeoniflorin, 0.005%-0.05% of salvianolic acid B, 0.001%-0.01% of polyhexamethylene biguanide hydrochloride, 0.3%-1.0% of a buffering agent, 0.01%-0.1% of a surfactant and the balance of water. The components interact with each other, and the texturing additive is added into the texturing liquid to improve the flatness of the texturing surface and effectively solve the problem that the texturing surface is not fully distributed, so that the minority carrier lifetime and the open voltage of the back contact battery are improved, and the photoelectric conversion efficiency of the battery is improved.

The invention discloses a method for determining chemical components in a medicine for treating vitiligo and a method for establishing a fingerprint spectrum. Comprising the following steps: S1, preparing a to-be-detected solution and a reference substance solution: grinding Bailing tablets, dissolving the Bailing tablets in a solvent, and extracting to prepare the to-be-detected solution; dissolving a paeoniflorin reference substance in a solvent to prepare a reference substance solution; wherein the extraction solvent is a methanol-water mixed solution; s2, respectively obtaining chromatograms of the to-be-detected solution and the reference substance solution under the same detection conditions by adopting ultra-high performance liquid chromatography, and carrying out qualitative analysis and / or quantitative analysis on chemical components in the chromatograms of the to-be-detected solution; wherein the detection conditions are as follows: the wavelength of a detector is 201 + / -2nm; the elution conditions are as follows: acetonitrile is used as a phase A, water is used as a phase B, and elution is performed through a gradient elution procedure. According to the method, the chemical components in the medicine for treating the leucoderma can be accurately determined, so that the establishment of the fingerprint spectrum database of the medicine for treating the leucoderma can be better realized.

The invention relates to the technical field of quality detection, and discloses a method for determining the content of five components of liver-soothing tablets based on a double-label multi-measurement method, and the method comprises the following steps: S1, extracting a test solution; s2, preparing a reference substance solution; s3, preparing each negative control solution; s4, determining chromatographic conditions; s5, constructing a retention time prediction model by using a double-marking linear correction method, taking the standard retention time of paeoniflorin and hesperidin as a horizontal coordinate, taking the actual retention time of paeoniflorin and hesperidin in the chromatographic column as a vertical coordinate, and substituting the standard retention time of agarotetrol, naringin and neohesperidin into the equation to calculate and obtain predicted retention time respectively; s6, determining the contents of paeoniflorin, agarotetrol, naringin, hesperidin and neohesperidin in the liver soothing tablet sample by adopting a double-label multi-determination method; according to the method, the contents of five components, namely paeoniflorin, agarotetrol, naringin, hesperidin and neohesperidin, in the liver-soothing tablet are simultaneously determined by a double-label multi-measurement method, the accuracy is high, and the cost is low.

The invention discloses a quality detection method of a gout clearing mixture for treating hyperuricemia, which adopts a separation analysis technology of ultra-high performance liquid chromatography to detect eight chemical components in the gout clearing mixture. Comprising the following components: 3-O-feruloylquinic acid, 5-O-caffeoylshikimic acid, paeoniflorin, polydatin, neoastilbin, astilbin, berberine hydrochloride and emodin-8-beta-D-glucopyranoside. According to the method, eight chemical components of six active components in the gout clearing mixture are quantitatively analyzed at the same time, and the method has the advantages of being easy and convenient to operate, good in stability and easy to popularize. The invention can provide a comprehensive and reliable quality control basis for the gout clearing mixture, and has important significance for ensuring the clinical curative effect of the gout clearing mixture.

The invention provides application of paeoniflorin in preparation of a medicine for inhibiting diabetic retinal vascular hyperplasia. The active ingredients of the medicine are one or more of paeoniflorin, a hydrate of paeoniflorin, pharmaceutically acceptable salt of paeoniflorin, a tautomer of paeoniflorin, a stereoisomer of paeoniflorin and a precursor compound of paeoniflorin. Paeoniflorin has potential to be developed into drugs, health care products or functional food for treating diabetic retinopathy.

The present invention relates to a composition of paeoniflorin and isoliquiritigenin and its application. The composition according to the present invention has rich raw materials, has effects such as anti-inflammatory, antioxidant, and inhibition of collagen deposition, can be effectively used for the treatment of diffuse pulmonary interstitial fibrosis, and has high economic and social benefits.

The invention provides a preparation method of traditional Chinese medicine hydrogel for treating psoriasis, the hydrogel preparation is prepared from the following raw material medicines in parts by weight: 20-40 parts of PVA, 60-80 parts of borax, 20-40 parts of glycyrrhizic acid, 8-12 parts of paeoniflorin and 2-3 parts of glycerin, the hydrogel has the advantages of injectability, self-healing, stretchability and the like, can meet the requirements of skin dressing, can be tightly attached to the skin, and has a good application prospect. The separation is easy. Animal experiments prove that after being loaded with paeoniflorin, the gel has the curative effect of treating mild and moderate psoriasis, the treatment effect is remarkable, and the gel has a wide market application prospect.

The invention relates to a method for extracting paeonol and paeoniflorin from fresh radix paeoniae rubra. The method comprises the following specific steps: washing and mincing the fresh radix paeoniae rubra, extracting with diethyl ether, performing alcohol extraction and water extraction, filtering, concentrating, performing alcohol precipitation, crystallizing and recrystallizing to obtain pure paeonol and paeoniflorin products. According to the method disclosed by the invention, diethyl ether extraction is adopted, so that not only is the enzyme activity of the fresh peony accelerated to be damaged, but also the risk that paeonol is oxidized is reduced, the leaching speed of paeonol is increased, the utilization efficiency of the fresh peony is greatly improved, the drying step of the fresh peony is effectively reduced, and the production energy consumption during extraction processing is saved; the dissolution and volatilization characteristics of paeonol are fully considered, diethyl ether removal and paeonol volatilization are carried out by utilizing different temperature gradients, and refining is carried out to obtain a pure paeonol product.

The invention discloses a preparation process of peony seed extract with high paeoniflorin and resveratrol content, and belongs to the technical field of paeoniflorin and resveratrol extraction. The technical problem to be solved is to improve the content of resveratrol and paeoniflorin in the peony seed extract. According to the technical scheme, peony seeds are smashed, soaked, subjected to alcohol extraction, filtered, concentrated and dried; a solvent for alcohol extraction is an ethanol solution with the volume fraction of 70-80%, and the solid-liquid ratio of alcohol extraction is 1g: (15-20) g.

The invention discloses an astragaloside-salvianolic acid B-paeoniflorin-amygdalin nano-liposome and a preparation method of the astragaloside-salvianolic acid B-paeoniflorin-amygdalin nano-liposome. The mass ratio of astragaloside to salvianolic acid B to paeoniflorin to amygdalin in the nano-liposome is (1-5): (1-5); (1-10): (1-5). The liposome disclosed by the invention can be used for preparing the medicine for preventing and / or treating cardiovascular diseases, especially for preparing the astragalus-dragon gum, and has a remarkable effect in the aspect of treating cardiovascular diseases.

The invention discloses a composition for inhibiting bacteria, resisting inflammation, relieving inflammation and repairing sensitive skin. The composition comprises the following components in parts by weight: 1-5 parts of a sophora flavescens extract; 2-15 parts of a radix scutellariae extract; 5-10 parts of a white peony root extract; 0.2 to 5 parts of a grapefruit extract; and 1-5 parts of pomegranate extract. The composition prepared from the radix sophorae flavescentis extract, the radix scutellariae extract, the radix paeoniae alba extract, the grapefruit extract and the pomegranate extract takes sophocarpidine, baicalin, paeoniflorin, naringin and punicalagin as main functional components to play a synergistic effect, so that the composition has very remarkable effects of inhibiting bacteria, resisting inflammation, relieving inflammation and repairing sensitive skin.

The invention provides a pharmaceutical composition for treating prostatitis and a preparation method thereof. The pharmaceutical composition is prepared from the following raw materials in parts by weight: 20 to 60 parts of palmatine, 20 to 60 parts of paeoniflorin, 10 to 30 parts of formononetin, 20 to 60 parts of astilbin and 10 to 40 parts of stigmasterol. In-vitro cell tests and in-vivo animal experiments prove that the pharmaceutical composition has the effect of relieving the chronic prostatitis reaction.

The invention discloses a platelet increasing capsule characteristic chromatogram and a construction method and application thereof, and belongs to the technical field of quality control of traditional Chinese medicine compound preparations. According to the method, 13 control components including gallic acid, catechin, paeoniflorin, forsythiaside B, benzoic acid, astragalus smicus glycoside, forsythin, arctiin, liquiritigenin, benzoyl paeoniflorin, paeonol, arctigenin and indirubin are selected, a high performance liquid chromatography-mass spectrometry technology is applied, multiple batches of preparation samples are analyzed, and the content of the components in the preparation samples is determined. And establishing a characteristic spectrum of the chemical components. Compared with a traditional method, the technology has good precision and reproducibility, the data result is accurate and reliable, meanwhile, the technology can identify the 13 components at the same time under the same chromatographic condition, the analysis efficiency is remarkably improved, synchronous monitoring of multiple effective components is achieved, and the consistency and stability of the preparation quality can be guaranteed.

The present invention discloses a multi-layer microsphere based on a hollow porous yeast carrier, its preparation method and application, belonging to the field of biomedical technology. The material comprises a hollow porous yeast carrier; the interior of the hollow porous yeast carrier is loaded with traditional Chinese medicine active molecules; the surface of the hollow porous yeast carrier is coated with a cross-linking agent layer; lactic acid bacteria are loaded on the cross-linking agent layer. The hollow porous yeast carrier is obtained by treating yeast successively with alkali, acid and organic solvent; the yeast is Saccharomyces cerevisiae; the traditional Chinese medicine active molecule is paeoniflorin; the cross-linking agent layer is formed by configuring a cross-linking agent into a solution at a pH value of 4.9 - 5.0 and cross-linking; the cross-linking agent is chitosan and sodium tripolyphosphate; the lactic acid bacteria are Lactobacillus paracasei. The microspheres of the present invention can not only inhibit the growth of Staphylococcus aureus, but also play an anti-inflammatory and repair role, reduce drug resistance, and improve the stability and targeting of drugs in the vaginal environment.

The invention discloses a novel application of total glucosides of paeony in preparation of a medicine for treating triple negative breast cancer. In-vitro and in-vivo experiments prove that the total glucosides of paeony can remarkably inhibit proliferation, migration and invasion of triple negative breast cancer cells (MDA-MB-231 and MDA-MB-468), reverse an epithelial-mesenchymal transition process and induce cell apoptosis by inducing mitochondrial injury, inhibiting oxidative phosphorylation and activating a mitochondrial apoptosis pathway. In an MDA-MB-231 cell xenotransplantation tumor nude mouse model, the total glucosides of paeony can effectively inhibit tumor growth. Therefore, the total glucosides of paeony can be used for preparing the medicine for treating the triple negative breast cancer, and a new treatment choice is provided for clinic.

The application provides a method for establishing HPLC fingerprint of Changnyle capsule (I), which is characterized in that 16 active components including gastrodin, danshensu, quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiopicroside, 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, puerarin apioside, paeoniflorin, daidzin, rosmarinic acid, shikonin, isoquercitrin, ononin, daidzein, salvianolic acid B and calycosin are used as reference substances, HPLC is adopted, methanol-acetonitrile-0.5vol% phosphoric acid aqueous solution is used as a mobile phase, different batches of samples are detected, and finally the fingerprint of Changnyle capsule (I) is obtained. The fingerprint can comprehensively represent the main components in Changnyle capsule (I), thereby being beneficial to the quality control of Changnyle capsule (I).

This invention relates to a highly active effective fraction of the Epiglottis-Clearing Decoction and its application in the preparation of antitussive drug compositions, belonging to the field of traditional Chinese medicine extract technology. The effective fraction contains hydroxysaffron yellow A, paeoniflorin, naringin, and neohesperidin, with the total content of these four components not less than 20% of the total mass of the effective fraction. The effective fraction of this invention is prepared by purification with macroporous resin. Compared with the freeze-dried powder of the traditional Epiglottis-Clearing Decoction, the total content of the four active ingredients is increased from 3.32% to 20.87%, achieving a more than 6-fold high-efficiency enrichment. Animal pharmacodynamic experiments show that the antitussive effect of a low dose (275.5 mg / kg) of this effective fraction is comparable to that of the freeze-dried powder of the original decoction (1928 mg / kg), reducing the dosage to about 1 / 7 of the original formula, demonstrating a significant "synergistic effect with reduced dosage" technical effect. The effective active ingredients provided by this invention have a clear content and stable quality, and can be made into modern preparations such as tablets, capsules or granules, solving the problems of large volume, inaccurate dosage and poor patient compliance of traditional decoctions.

The application discloses a kind of self-assembly hydrogel, preparation method and application, belong to material field.The application claims to protect a kind of self-assembly hydrogel, it is formed by glycyrrhizic acid, five gallate glucose and paeonol by molecular self-assembly;Wherein, the mass ratio of glycyrrhizic acid and five gallate glucose, paeonol is (10~60) :(1~8) :(2~8).The self-assembly hydrogel provided by the application is suitable for skin, raw material is natural, biocompatibility is good, mechanical and storage stability is excellent, can release paeonol and the transdermal effect is good, three components synergistically improve atopic dermatitis and other skin inflammation, and clinical value is high.In addition, the preparation process of the self-assembly hydrogel of the application is simple, can be prepared using conventional one-pot method, easy to produce.Therefore, the self-assembly hydrogel of the application has the prospect of developing into paeonol sustained-release delivery system, paeonol transdermal sustained-release preparation or skin inflammation treatment drug.

The application provides a pharmaceutical composition for treating rheumatoid arthritis, which is composed of paeoniflorin, glycyrrhizin, glycyrrhizic acid and glycyrrhetic acid in a weight ratio of (4.5-7.5):(0.4-1.8):(1.8-4.3):(22-40). The pharmaceutical composition can significantly inhibit the proliferation of fibroblast-like synoviocytes, reduce the secretion of inflammation-related factors (TNF-alpha, IL-1beta, IL-6 and COX-2), regulate the expression of TNF-alpha / NF-kappaB signal pathway-related proteins, and has a significant curative effect on rheumatoid arthritis.

The invention provides a method for preparing paeoniflorin and albiflorin by utilizing supercritical chromatography, and belongs to the field of natural products. The invention provides a method for preparing paeoniflorin and albiflorin by using supercritical chromatography, which comprises the following steps: carrying out supercritical chromatography analysis on a solution of a sample to obtain a chromatogram of the sample; if the chromatogram shows that the sample contains paeoniflorin and albiflorin, carrying out supercritical chromatographic separation on the solution of the sample to obtain pure paeoniflorin and pure albiflorin; a chromatographic column used for the supercritical chromatographic analysis and the supercritical chromatographic separation is silica. The method is simple, convenient and efficient, simple in pretreatment, high in analysis and separation speed, small in used solvent amount, easy to recover and reusable, time, manpower and material resources are saved, the purity of paeoniflorin and albiflorin is improved by selecting silica as a chromatographic column, the single sample loading amount is large, and high-purity, rapid and large-scale preparation of paeoniflorin and albiflorin can be achieved.

The centrifugal separator comprises a centrifugal box body and a digital display control panel, a centrifugal chamber is formed in the centrifugal box body, the upper side of the centrifugal chamber is open, and a centrifugal barrel structure is rotationally arranged in the centrifugal chamber; a second motor for driving the centrifugal barrel structure to rotate is arranged on the bottom side of the centrifugal box body; a box cover is arranged at an opening in the upper side of the centrifugal box body, a lifting driving assembly used for driving the box cover to ascend and descend vertically is arranged on the centrifugal box body, and when the lifting driving assembly drives the box cover to descend to the lowest position height, the box cover can seal the opening in the upper side of the centrifugal box body. The adjustable filter gaps are arranged, so that the separation conditions can be flexibly adjusted according to the sizes of impurity particles, the separation efficiency and the extraction purity of various components of paeoniflorin are remarkably improved, and the requirements of the fields of medicines, health care products and the like on high-purity paeoniflorin are met.

The present invention discloses a method for simultaneously detecting seven effective ingredients in Yangyin Qingfei Pills, and belongs to the technical field of chemical detection. The method for simultaneously detecting seven effective ingredients in Yangyin Qingfei Pills described in the present invention comprises the following steps: injecting the Yangyin Qingfei Pill sample solution into a liquid chromatograph, and using a segmented wavelength conversion method to perform chromatographic detection to achieve qualitative and quantitative detection of the seven effective ingredients. The present invention uses the UPLC segmented wavelength conversion method to achieve simultaneous determination of the contents of harpagine, paeoniflorin, liquiritin, verbascoside, harpagine, paeonol and glycyrrhizic acid in Yangyin Qingfei Pills, and all seven effective ingredients can be well separated without interference from other ingredients. Moreover, each component has a good linear relationship within its own linear range, and the sample recovery rate is between 95% and 105%. The detection method of the present invention is simple, accurate and effective, and can be used for quality control and overall quality evaluation of Yangyin Qingfei Pills.

The invention provides application of a paeoniflorin photoaffinity probe in screening key target protein of paeoniflorin, and relates to the technical field of chemical proteomics. The paeoniflorin photoaffinity probe has a structure as shown in a formula I, and the paeoniflorin key target proteins are 40S ribosomal protein S3 and statin 2. The paeoniflorin photoaffinity probe is applied to screening of the paeoniflorin key target proteins, so that two target proteins combined with paeoniflorin in liver cells are successfully identified; the two target proteins are respectively 40S ribosomal protein S3 (RPS3) and statin 2 (PHB2), and the two target proteins participate in the protection effect of paeoniflorin on the liver, and have the key effects of oxidation resistance, inflammation resistance, immunoregulation and the like in liver diseases.

The invention belongs to the technical field of biology and new medicine, and discloses a preparation method of acoustic resonance driven glycyrrhizic acid nano-composite responsive gel, glycyrrhizic acid is used as a drug carrying system, berberine, ursolic acid, baicalein, luteolinin, curcumin, baicalin or ginsenoside Rh2 is used as a water-insoluble natural small molecule drug, and the acoustic resonance driven glycyrrhizic acid nano-composite responsive gel is prepared. Glycyrrhizic acid is an amphiphilic compound composed of one molecule of glycyrrhetinic acid and two molecules of glucuronic acid, a hydrophobic cavity can be formed in a water environment to wrap the water-insoluble natural small molecule medicine, a glycyrrhizic acid nano-composite solution is formed, and meanwhile the solubility of the water-insoluble natural small molecule medicine such as baicalein is improved.

The present invention provides a method for detecting the content of anti-inflammatory active ingredients in a rhubarb and peony preparation. The method utilizes specific solvents and chromatographic conditions, and employs multi-wavelength switching technology to simultaneously and quantitatively detect nine anti-inflammatory active ingredients in rhubarb and peony granules. The method is simple to operate, has good stability, high accuracy, short detection time, high efficiency, and low detection cost. The method calculates the content of the main active ingredients in the rhubarb and peony preparation, including gallic acid, amygdalin, paeoniflorin, paeonol, aloe-emodin, rhein, emodin, chrysophanol, and physcion, by calculating the relative correction factor f. This method provides a more comprehensive method for quality control of rhubarb and peony preparations.

The present invention provides a method for constructing a fingerprint of a traditional Chinese medicine preparation for treating eczema, comprising the following steps: taking gallic acid, gentiopicroside, geniposide, paeoniflorin, ammonium glycyrrhizate, salvianolic acid B, baicalin, baicalein and wogonin reference substances to prepare a mixed reference substance solution; mixing the traditional Chinese medicine preparation for treating eczema with an organic solvent to prepare a test solution; the organic solvent is selected from one or more of methanol aqueous solution, ethanol aqueous solution and anhydrous ethanol; the mixed reference substance solution and the test solution are respectively subjected to high performance liquid chromatography determination; wherein the conditions adopted by the high performance liquid chromatography include: mobile phase A is selected from one or more of methanol and acetonitrile, and mobile phase B is selected from one or more of phosphoric acid aqueous solution, acetic acid aqueous solution and formic acid aqueous solution; and a gradient elution program is adopted.

The invention discloses bacillus subtilis BS4-35 and an application thereof in preparation of a white peony root whitening aqueous extract. The bacillus subtilis BS4-35 is preserved in Guangdong Microbial Culture Collection Center, the preservation number is GDMCC No: 65591, and the preservation date is December 9, 2024. Before the white paeony root whitening aqueous extract is extracted by a hot water ultrasonic method, the bacillus subtilis BS4-35 is added for fermentation treatment, so that the yield of the aqueous extract is remarkably improved, and the activity of inhibiting tyrosinase is also remarkably enhanced. Compared with an unfermented direct hot water ultrasonic extraction method, the yield of the aqueous extract of the radix paeoniae alba can be increased by 31.1%. The contents of polysaccharide and paeoniflorin in the aqueous extract are respectively increased by 18.5% and 11.4%, and the activity of inhibiting tyrosinase is enhanced by 25.3%.