107 results about "Paeoniflorin" patented technology

The invention belongs to the technical field of solar cells, and particularly discloses a texturing additive, a texturing solution and a texturing method of a back contact cell. The texturing additive for the back contact battery comprises the following components in percentage by mass: 1.0%-5.0% of sodium carboxymethyl cellulose, 0.03%-0.2% of sodium lignin sulfonate, 0.003%-0.03% of paeoniflorin, 0.005%-0.05% of salvianolic acid B, 0.001%-0.01% of polyhexamethylene biguanide hydrochloride, 0.3%-1.0% of a buffering agent, 0.01%-0.1% of a surfactant and the balance of water. The components interact with each other, and the texturing additive is added into the texturing liquid to improve the flatness of the texturing surface and effectively solve the problem that the texturing surface is not fully distributed, so that the minority carrier lifetime and the open voltage of the back contact battery are improved, and the photoelectric conversion efficiency of the battery is improved.

The invention provides a preparation method of traditional Chinese medicine hydrogel for treating psoriasis, the hydrogel preparation is prepared from the following raw material medicines in parts by weight: 20-40 parts of PVA, 60-80 parts of borax, 20-40 parts of glycyrrhizic acid, 8-12 parts of paeoniflorin and 2-3 parts of glycerin, the hydrogel has the advantages of injectability, self-healing, stretchability and the like, can meet the requirements of skin dressing, can be tightly attached to the skin, and has a good application prospect. The separation is easy. Animal experiments prove that after being loaded with paeoniflorin, the gel has the curative effect of treating mild and moderate psoriasis, the treatment effect is remarkable, and the gel has a wide market application prospect.

The invention discloses a preparation process of peony seed extract with high paeoniflorin and resveratrol content, and belongs to the technical field of paeoniflorin and resveratrol extraction. The technical problem to be solved is to improve the content of resveratrol and paeoniflorin in the peony seed extract. According to the technical scheme, peony seeds are smashed, soaked, subjected to alcohol extraction, filtered, concentrated and dried; a solvent for alcohol extraction is an ethanol solution with the volume fraction of 70-80%, and the solid-liquid ratio of alcohol extraction is 1g: (15-20) g.

The invention discloses a composition for inhibiting bacteria, resisting inflammation, relieving inflammation and repairing sensitive skin. The composition comprises the following components in parts by weight: 1-5 parts of a sophora flavescens extract; 2-15 parts of a radix scutellariae extract; 5-10 parts of a white peony root extract; 0.2 to 5 parts of a grapefruit extract; and 1-5 parts of pomegranate extract. The composition prepared from the radix sophorae flavescentis extract, the radix scutellariae extract, the radix paeoniae alba extract, the grapefruit extract and the pomegranate extract takes sophocarpidine, baicalin, paeoniflorin, naringin and punicalagin as main functional components to play a synergistic effect, so that the composition has very remarkable effects of inhibiting bacteria, resisting inflammation, relieving inflammation and repairing sensitive skin.

The invention provides a pharmaceutical composition for treating prostatitis and a preparation method thereof. The pharmaceutical composition is prepared from the following raw materials in parts by weight: 20 to 60 parts of palmatine, 20 to 60 parts of paeoniflorin, 10 to 30 parts of formononetin, 20 to 60 parts of astilbin and 10 to 40 parts of stigmasterol. In-vitro cell tests and in-vivo animal experiments prove that the pharmaceutical composition has the effect of relieving the chronic prostatitis reaction.

The invention discloses a platelet increasing capsule characteristic chromatogram and a construction method and application thereof, and belongs to the technical field of quality control of traditional Chinese medicine compound preparations. According to the method, 13 control components including gallic acid, catechin, paeoniflorin, forsythiaside B, benzoic acid, astragalus smicus glycoside, forsythin, arctiin, liquiritigenin, benzoyl paeoniflorin, paeonol, arctigenin and indirubin are selected, a high performance liquid chromatography-mass spectrometry technology is applied, multiple batches of preparation samples are analyzed, and the content of the components in the preparation samples is determined. And establishing a characteristic spectrum of the chemical components. Compared with a traditional method, the technology has good precision and reproducibility, the data result is accurate and reliable, meanwhile, the technology can identify the 13 components at the same time under the same chromatographic condition, the analysis efficiency is remarkably improved, synchronous monitoring of multiple effective components is achieved, and the consistency and stability of the preparation quality can be guaranteed.

The invention discloses a novel application of total glucosides of paeony in preparation of a medicine for treating triple negative breast cancer. In-vitro and in-vivo experiments prove that the total glucosides of paeony can remarkably inhibit proliferation, migration and invasion of triple negative breast cancer cells (MDA-MB-231 and MDA-MB-468), reverse an epithelial-mesenchymal transition process and induce cell apoptosis by inducing mitochondrial injury, inhibiting oxidative phosphorylation and activating a mitochondrial apoptosis pathway. In an MDA-MB-231 cell xenotransplantation tumor nude mouse model, the total glucosides of paeony can effectively inhibit tumor growth. Therefore, the total glucosides of paeony can be used for preparing the medicine for treating the triple negative breast cancer, and a new treatment choice is provided for clinic.

The application provides a method for establishing HPLC fingerprint of Changnyle capsule (I), which is characterized in that 16 active components including gastrodin, danshensu, quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiopicroside, 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, puerarin apioside, paeoniflorin, daidzin, rosmarinic acid, shikonin, isoquercitrin, ononin, daidzein, salvianolic acid B and calycosin are used as reference substances, HPLC is adopted, methanol-acetonitrile-0.5vol% phosphoric acid aqueous solution is used as a mobile phase, different batches of samples are detected, and finally the fingerprint of Changnyle capsule (I) is obtained. The fingerprint can comprehensively represent the main components in Changnyle capsule (I), thereby being beneficial to the quality control of Changnyle capsule (I).

This invention relates to a highly active effective fraction of the Epiglottis-Clearing Decoction and its application in the preparation of antitussive drug compositions, belonging to the field of traditional Chinese medicine extract technology. The effective fraction contains hydroxysaffron yellow A, paeoniflorin, naringin, and neohesperidin, with the total content of these four components not less than 20% of the total mass of the effective fraction. The effective fraction of this invention is prepared by purification with macroporous resin. Compared with the freeze-dried powder of the traditional Epiglottis-Clearing Decoction, the total content of the four active ingredients is increased from 3.32% to 20.87%, achieving a more than 6-fold high-efficiency enrichment. Animal pharmacodynamic experiments show that the antitussive effect of a low dose (275.5 mg / kg) of this effective fraction is comparable to that of the freeze-dried powder of the original decoction (1928 mg / kg), reducing the dosage to about 1 / 7 of the original formula, demonstrating a significant "synergistic effect with reduced dosage" technical effect. The effective active ingredients provided by this invention have a clear content and stable quality, and can be made into modern preparations such as tablets, capsules or granules, solving the problems of large volume, inaccurate dosage and poor patient compliance of traditional decoctions.

The application discloses a kind of self-assembly hydrogel, preparation method and application, belong to material field.The application claims to protect a kind of self-assembly hydrogel, it is formed by glycyrrhizic acid, five gallate glucose and paeonol by molecular self-assembly;Wherein, the mass ratio of glycyrrhizic acid and five gallate glucose, paeonol is (10~60) :(1~8) :(2~8).The self-assembly hydrogel provided by the application is suitable for skin, raw material is natural, biocompatibility is good, mechanical and storage stability is excellent, can release paeonol and the transdermal effect is good, three components synergistically improve atopic dermatitis and other skin inflammation, and clinical value is high.In addition, the preparation process of the self-assembly hydrogel of the application is simple, can be prepared using conventional one-pot method, easy to produce.Therefore, the self-assembly hydrogel of the application has the prospect of developing into paeonol sustained-release delivery system, paeonol transdermal sustained-release preparation or skin inflammation treatment drug.

The application provides a pharmaceutical composition for treating rheumatoid arthritis, which is composed of paeoniflorin, glycyrrhizin, glycyrrhizic acid and glycyrrhetic acid in a weight ratio of (4.5-7.5):(0.4-1.8):(1.8-4.3):(22-40). The pharmaceutical composition can significantly inhibit the proliferation of fibroblast-like synoviocytes, reduce the secretion of inflammation-related factors (TNF-alpha, IL-1beta, IL-6 and COX-2), regulate the expression of TNF-alpha / NF-kappaB signal pathway-related proteins, and has a significant curative effect on rheumatoid arthritis.

The invention provides application of a paeoniflorin photoaffinity probe in screening key target protein of paeoniflorin, and relates to the technical field of chemical proteomics. The paeoniflorin photoaffinity probe has a structure as shown in a formula I, and the paeoniflorin key target proteins are 40S ribosomal protein S3 and statin 2. The paeoniflorin photoaffinity probe is applied to screening of the paeoniflorin key target proteins, so that two target proteins combined with paeoniflorin in liver cells are successfully identified; the two target proteins are respectively 40S ribosomal protein S3 (RPS3) and statin 2 (PHB2), and the two target proteins participate in the protection effect of paeoniflorin on the liver, and have the key effects of oxidation resistance, inflammation resistance, immunoregulation and the like in liver diseases.

The invention belongs to the technical field of biology and new medicine, and discloses a preparation method of acoustic resonance driven glycyrrhizic acid nano-composite responsive gel, glycyrrhizic acid is used as a drug carrying system, berberine, ursolic acid, baicalein, luteolinin, curcumin, baicalin or ginsenoside Rh2 is used as a water-insoluble natural small molecule drug, and the acoustic resonance driven glycyrrhizic acid nano-composite responsive gel is prepared. Glycyrrhizic acid is an amphiphilic compound composed of one molecule of glycyrrhetinic acid and two molecules of glucuronic acid, a hydrophobic cavity can be formed in a water environment to wrap the water-insoluble natural small molecule medicine, a glycyrrhizic acid nano-composite solution is formed, and meanwhile the solubility of the water-insoluble natural small molecule medicine such as baicalein is improved.

The invention discloses a method for determining chemical components in a medicine for treating vitiligo, which relates to the technical field of medicine analysis, and is characterized in that ultra-high performance liquid chromatography-mass spectrometry is adopted to determine the content of chemical components in a Bailing tablet: psoralen, isopsoralen, astragaloside, quercetin, polysaccharide and amino acid. The determination method comprises the following steps: respectively injecting a reference solution and a test solution into a liquid chromatograph for determination to obtain a chromatogram, and calculating the content of paeoniflorin in a test sample by adopting an external standard method, chromatographic conditions of a chromatogram obtained by adopting a liquid chromatograph are as follows: octadecylsilane chemically bonded silica is taken as a filling agent. According to the method for determining the chemical components in the medicine for treating leucoderma, the established detection method is verified, and the result shows that the detection method is easy and convenient to operate, accurate in result, high in specificity and good in reproducibility.

The application provides a method for detecting the pharmacodynamic components of a traditional Chinese medicine composition by UPLC-MS / MS. The method can identify 14 pharmacodynamic components of the traditional Chinese medicine composition, including saikosaponin A, saikosaponin D, naringin, paeoniflorin, hesperidin, chicoric acid, chlorogenic acid, salvianolic acid B, zephyranthine, kaempferitrin, and codariusrhizome glycoside, amargin, hyperoside and rutin, thereby providing a reference for evaluating the intrinsic quality of the traditional Chinese medicine composition.

The application discloses a freeze-dried mask based on Yuyong Powder and a preparation method thereof, and the preparation method comprises the following steps: after raw materials of the Yuyong Powder are crushed at low temperature, the raw materials are extracted at low temperature by using a composite solvent, and then the extracted solution is refined by flocculation clarification, centrifugation and microfiltration to obtain a refined extraction solution; the refined extraction solution is mixed with a humectant and a freeze-drying protective agent, and then is injected into a mask support, and gradient temperature control freeze-drying is carried out; finally, the freeze-dried mask is sealed and packaged under sterile conditions by using nitrogen. Through the whole low-temperature process and the synergistic effect of the composite freeze-drying protective agent, the heat-sensitive whitening components such as paeoniflorin, salvianolic acid and glabridin in the Yuyong Powder are effectively retained; impurities are removed through three-stage refining and purification, so that the irritability of the product is reduced; through freeze-drying and high-barrier packaging, the product can be stored at room temperature for a long time. The obtained freeze-dried mask can be quickly reconstituted when meeting water, has a refreshing skin feeling, and has remarkable effects of removing yellow and brown spots and whitening, and has the advantages of high activity, high stability, high convenience and high safety.

The invention belongs to the technical field of medicines, and particularly relates to an active ingredient composition for treating xerophthalmia and muscae volitantes as well as a preparation method and application of the active ingredient composition. The composition is prepared from lycium barbarum polysaccharide, zeaxanthine, apigenin, luteolin, zinc gluconate, ophiopogon japonicus polysaccharide, ophiopogonin B, paeoniflorin and gallic acid according to a specific weight part ratio, all the active ingredients have a synergistic effect, the ocular surface microenvironment is improved, the vitreous opacity progress is inhibited, eye tissue damage is repaired, and the eye quality is improved. The synergistic treatment effect on the xerophthalmia and the muscae volitantes is achieved, and the symptoms such as eye dryness, eye acerbity, lacrimal secretion insufficiency, black shadow fluttering and blurred vision can be effectively relieved.

The application belongs to the field of natural product extraction and cosmetic raw materials, and discloses a preparation method and application of a composition containing asiaticoside-paeoniflorin, which comprises two key and functionally different stages: ① extraction stage: using asiaticoside as an extraction aid and using propylene glycol as a complex medium, efficiently dissolving and forming an "asiaticoside-paeoniflorin" supramolecular complex; ② complexing and stabilizing stage: using glycerol as a stabilizer to maintain the long-term kinetic stability of the formed supramolecular complex and prevent its dissociation and aggregation. The method solves the technical problem that the extraction rate and stability of paeoniflorin are difficult to be considered simultaneously in the field by functional division of extraction and stabilization, and the composition containing asiaticoside-paeoniflorin obtained finally has excellent soothing performance, not only rapid effect, but also persistent and stable soothing protection effect, which can effectively resist and repair the influence of external stimulation on the skin.

The present application relates to the field of pharmacology, in particular to the use of paeoniflorin-6'-O-benzene sulfonate for improving acinar atrophy, maintaining the stability of gland functional units, and repairing gland secretion function. The paeoniflorin-6'-O-benzene sulfonate can effectively improve the acinar atrophy of diabetic animals, and the effect is significantly better than that of paeoniflorin, and can be used for preparing a medicine for treating xeroma.

The invention belongs to the field of analysis of traditional Chinese medicine preparations, and particularly discloses a method for determining the content of 15 components in a Xiaoyao preparation through quantitative analysis of multi-components by single marker and application of the method. According to the invention, pentagalloylglucose is used as an internal reference substance for quantitative analysis of multi-components by single marker; relative correction factors between the pentagalloylglucose and gallic acid, chlorogenic acid, albiflorin, paeoniflorin, schaftoside, ferulic acid, apiose liquiritin, liquiritin, senkyunolide I, paeoniflorin, senkyunolide H, formononin, liquiritigenin and glycyrrhizic acid in the Xiaoyao preparation are established, and the contents of the 15 components in the Xiaoyao preparation are calculated through the correction factors. The method disclosed by the invention is high in practicability, simple to operate, cost-saving and accurate and reliable in detection result, overcomes the defects of the quality detection method of the Xiaoyao preparation, especially the Xiaoyao mixture, and provides a new way for quality control of the Xiaoyao preparation and internal quality evaluation of medicines.

This invention discloses the application of total paeoniflorin in the preparation of drugs for the treatment of head and neck squamous cell carcinoma, belonging to the field of biomedical technology. Addressing the bottlenecks in current treatments for head and neck squamous cell carcinoma, such as high toxicity of radiotherapy and chemotherapy, low response rates to immunotherapy, and easy resistance to targeted drugs, this invention provides a method for preparing total paeoniflorin and a drug-loaded preparation technology based on hydrogels obtained from modified chitosan. Total paeoniflorin can dose-dependently inhibit the proliferation rate, colony formation ability, and in vitro migration ability of SCC7, FADU, and CAL27 head and neck squamous cell carcinoma cell lines, and significantly induce apoptosis. This invention utilizes the multi-target effects of total paeoniflorin to exert anti-head and neck squamous cell carcinoma effects through modulation of the tumor microenvironment and enhancement of immune surveillance. Furthermore, the loaded drug exhibits good sustained-release properties and biosafety, providing a new, highly effective, and low-toxicity strategy for the treatment of head and neck squamous cell carcinoma, and has significant clinical application value.

The invention discloses a screening method of anti-cerebral ischemic injury active components of a salvia miltiorrhiza-cortex moutan medicine, a targeted brain-entering composition and application thereof, and belongs to the technical field of traditional Chinese medicines. The pharmaceutical composition for resisting cerebral ischemic injury is prepared by compatibility of radix salviae miltiorrhizae and cortex moutan, blood-entering active components of the pharmaceutical composition comprise various components such as gallic acid, and paeoniflorin and the like are targeted brain-entering components. The invention also provides a method for screening the active ingredients of the composition in blood, which comprises the following steps: preparing a test sample and a mixed reference substance solution, and analyzing and determining the ingredients by high performance liquid chromatography and high resolution mass spectrometry. The composition can be used for preparing drugs for resisting cerebral ischemia injury, can improve the neurological function of rats and the like, and is suitable for treating acute and chronic cerebral ischemia and reperfusion injury. In addition, the active ingredients entering blood can be used for preparing drugs for promoting angiogenesis of cerebral vessels, and the BMECs related capacity of OGD / R damaged rats is improved. The invention provides a new direction and choice for treatment of cerebral ischemia injury.

The application discloses a detection method of Yichangleng composition, and the method comprises the following steps: S1, preparation of reference solution; S2, preparation of test solution; S3, preparation of mixed control solution; S4, respectively, precisely sucking Yichangleng test solution of S2 and mixed control solution of S3; injecting into a liquid chromatograph; S5, exporting Yichangleng characteristic spectrum obtained in S4, selecting chromatographic peaks existing in chromatograms of Yichangleng solution as characteristic peaks; calculating relative peak area and relative retention time of each characteristic peak; and according to the retention time of the chromatogram of the mixed control solution, labeling the components of the peaks in the control characteristic spectrum, the method can be used for detecting gallic acid, vanillic acid, paeoniflorin, uncarine, thalictrifolin, 5-O-methylvisamminol glycoside, quercetin, kaempferol and atractylochide II, and can realize overall quality control of Yichangleng solution.

The invention belongs to the technical field of traditional Chinese medicine preparations, and provides anti-cold granules and a preparation method thereof. The preparation method comprises the following steps: (1) preparing an extract: performing ultrasonic water extraction on honeysuckle flower powder to obtain a honeysuckle flower extract; performing ultrasonic extraction on the radix paeoniae rubra powder by adopting a composite solvent to obtain a radix paeoniae rubra water extract; adding a natural clarifying agent into the red paeony root water extract for clarifying, cooling, filtering, and concentrating under reduced pressure to obtain a red paeony root extract; carrying out ultrasonic water extraction on rhizoma dryopteris crassirhizomae, filtering, and carrying out vacuum concentration to obtain a rhizoma dryopteris crassirhizomae extract; (2) mixing the honeysuckle extract, the radix paeoniae rubra extract and the rhizoma dryopteris crassirhizomae extract, and performing vacuum concentration to obtain clear paste; and adding auxiliary materials into the clear paste to prepare the anti-cold granules. By adopting the preparation method, the dissolution of effective components in the anti-cold granules can be remarkably improved, and particularly the contents of paeoniflorin and chlorogenic acid are increased, so that the liver toxicity generated by the medicinal component phloroglucinol derivative of the rhizoma dryopteris crassirhizomae is balanced, and the effects of reducing toxicity and enhancing efficiency are achieved.

The application belongs to the technical field of content detection, and discloses a kind of HPLC detection methods for simultaneously determining the content of multiple components in semen Cannabis capsules.The detection method includes mixing reference solution, chrysophanol and methanol to obtain mixed reference solution.The reference solution includes physcion solution, magnolol solution, houpo chrysophanol solution, emodin solution, rhein solution, nobiletin solution, aloe emodin solution, hesperidin solution and paeoniflorin solution;The contents of semen Cannabis capsules are mixed with methanol, and the test solution is obtained after heating and refluxing;The mixed reference solution and test solution are respectively sucked and injected into high performance liquid chromatograph.The detection method can simultaneously determine the content of multiple index components, can comprehensively reflect the quality of the preparation, and the test method has excellent precision, repeatability, stability and high sample recovery rate.

The invention discloses a method for simultaneously determining seven mass markers (Q-Marker) in serum of traditional Chinese medicine particles for treating cervical pain, which is characterized in that the seven mass markers are magnoflorine, puerarin, tetrahydropalmatine, paeoniflorin, notoginsenoside R1, ligustilide and decuricin; the method comprises the following steps: adding a serum sample into acetonitrile and acetone, carrying out vortex and centrifugation, taking a supernatant, loading the supernatant into a Hybrid SPEcolumn, washing with water to remove salt and polar interferents, eluting a target analyte with acetonitrile, filtering the eluent through a microfiltration membrane, carrying out sample introduction determination, and carrying out quantitative analysis by adopting a high performance liquid chromatography-mass spectrometry method and an electrospray ion source. And quantitative analysis is carried out in a positive / negative ion multi-reaction monitoring detection mode. The method is efficient, sensitive and high in selectivity, and solves the problems of serious matrix interference, indefinite target components, difficulty in simultaneous analysis of multiple components and the like in the prior art.

The invention relates to the technical field of chemical analysis, in particular to a construction method and device of a near-infrared spectrogram analysis model and computer equipment, and the construction method of the near-infrared spectrogram analysis model comprises the steps that a data set is acquired, and the data set comprises a plurality of near-infrared spectrograms; a Lasso regression algorithm is adopted to construct a regression model; and training the regression model by using the data set to obtain an analysis model of the near infrared spectrogram. A near infrared spectrum data set is modeled and trained by adopting a Lasso regression algorithm, the linear relation between spectral characteristics and paeoniflorin concentration is effectively mined, and the constructed model not only has good generalization ability and stability, but also has relatively high interpretability and high calculation efficiency; experimental results show that the overfitting risk of the linear model is lower than 0.001, which is significantly superior to that of a nonlinear model, so that the practicability and reliability of the model are greatly improved while the prediction precision is ensured.

The invention relates to the technical field of cosmetics, in particular to a whitening, skin-tendering and anti-aging composition and a preparation method and application thereof.The whitening, skin-tendering and anti-aging composition is composed of the following components: a core active component: a peony root bark extract, the paeonol content of which is larger than or equal to 10% and the paeoniflorin content of which is larger than or equal to 8% (HPLC (High Performance Liquid Chromatography) detection); the solvent / carrier component is 1, 3-butanediol which is used as a compatible component of an extraction solvent and a cosmetic matrix; through efficient extraction with the 1, 3-butanediol and purification with a 5000Da ultrafiltration membrane, the content of paeonol is larger than or equal to 10%, the content of paeoniflorin is larger than or equal to 8%, and the content of paeonol and the content of paeoniflorin are obviously higher than those of commercially available peony extracts (generally paeonol is smaller than or equal to 5%), traditional ethyl alcohol is replaced with the 1, 3-butanediol, and therefore the paeoniflorin and paeoniflorin can be used for preparing the peony extract. The composition is low in irritation and good in compatibility with a cosmetic matrix, and is suitable for sensitive skin, and LD50 is greater than 2000mg / kg according to a skin irritation test.

The invention belongs to the technical field of acne rosacea treatment, and discloses a preparation method and application of a composition for treating acne rosacea, the composition for treating acne rosacea contains a moutan bark extract and a coptis chinensis extract, and also contains a lucid ganoderma extract, a cinnamon extract, paeoniflorin and syringic acid. The inflammatory response of rosacea is closely related to the expression of two core inflammatory factors, namely TNF-alpha and TLR4, and the invention consciously aims at the TLR4-TNF-alpha inflammatory axis multi-target design, inhibits the expression of TLR4 and TNF-alpha, and blocks the'start-amplification 'chain of inflammation, thereby inhibiting the recurrence of inflammation. In addition, the composition for treating rosacea provided by the invention can also improve capillary dysfunction, effectively inhibit microbial growth, and prevent and treat rosacea from multiple dimensions.

The application discloses a method for simultaneously controlling the qualitative and quantitative overall quality of a Huangqi Guizhi Wuwu Decoction (HGWD) reference sample, and the method is characterized in that: a water decoction process optimized through orthogonal tests is used to prepare HGWD decoctions and freeze-dried powder reference samples of multiple batches of medicinal material combinations from different producing areas; an HPLC characteristic chromatogram is established, 15 common peaks are calibrated by taking cinnamyl aldehyde as a reference peak; based on the same reference sample, the contents of eight index components, i.e., gallic acid, peony lactone glycoside, paeoniflorin, calycosin-7-glucoside, cinnamic acid, cinnamyl aldehyde, formononetin and 6-gingerol, are simultaneously determined by using a multi-wavelength HPLC, and the content of astragaloside A is determined by using an HPLC-ELSD method; the qualitative and quantitative overall quality of the reference sample is simultaneously controlled through characteristic chromatogram similarity evaluation and multi-index component content consistency evaluation. The application realizes the whole-process value transmission evaluation from medicinal material raw materials to final products, and provides a scientific basis for formulating the quality standard of the HGWD reference sample and developing granules.