143 results about "Paeoniflorin" patented technology

The invention discloses a planting method for increasing the content of anthocyanin in purple rice grains, which comprises the following specific steps: sowing purple rice seeds from May 20th to May 30th every year, and setting the nitrogen application amount to be 24kg N / mu and the planting density to be 1.58-2.22 million holes / mu at the same time. According to the method, the sowing time, the nitrogen application amount and the planting density are precisely and cooperatively regulated and controlled, resource distribution in the key period of purple rice anthocyanin synthesis is directionally optimized, the content of paeoniflorin and cyanidin in grains is remarkably increased, meanwhile, yield stability and efficient resource utilization are both considered, and through cultivation technology innovation which is low in cost and easy to popularize, the yield of purple rice is increased. The multi-target of directional enhancement of functional components of purple rice, stable yield and high resource efficiency is achieved, a technical normal form is provided for green transformation of functional agriculture, remarkable economic, ecological and social benefits are achieved, and the important requirement for green and high-efficiency development of functional agriculture is met.

The invention belongs to the technical field of solar cells, and particularly discloses a texturing additive, a texturing solution and a texturing method of a back contact cell. The texturing additive for the back contact battery comprises the following components in percentage by mass: 1.0%-5.0% of sodium carboxymethyl cellulose, 0.03%-0.2% of sodium lignin sulfonate, 0.003%-0.03% of paeoniflorin, 0.005%-0.05% of salvianolic acid B, 0.001%-0.01% of polyhexamethylene biguanide hydrochloride, 0.3%-1.0% of a buffering agent, 0.01%-0.1% of a surfactant and the balance of water. The components interact with each other, and the texturing additive is added into the texturing liquid to improve the flatness of the texturing surface and effectively solve the problem that the texturing surface is not fully distributed, so that the minority carrier lifetime and the open voltage of the back contact battery are improved, and the photoelectric conversion efficiency of the battery is improved.

The invention discloses a method for determining chemical components in a medicine for treating vitiligo and a method for establishing a fingerprint spectrum. Comprising the following steps: S1, preparing a to-be-detected solution and a reference substance solution: grinding Bailing tablets, dissolving the Bailing tablets in a solvent, and extracting to prepare the to-be-detected solution; dissolving a paeoniflorin reference substance in a solvent to prepare a reference substance solution; wherein the extraction solvent is a methanol-water mixed solution; s2, respectively obtaining chromatograms of the to-be-detected solution and the reference substance solution under the same detection conditions by adopting ultra-high performance liquid chromatography, and carrying out qualitative analysis and / or quantitative analysis on chemical components in the chromatograms of the to-be-detected solution; wherein the detection conditions are as follows: the wavelength of a detector is 201 + / -2nm; the elution conditions are as follows: acetonitrile is used as a phase A, water is used as a phase B, and elution is performed through a gradient elution procedure. According to the method, the chemical components in the medicine for treating the leucoderma can be accurately determined, so that the establishment of the fingerprint spectrum database of the medicine for treating the leucoderma can be better realized.

The invention relates to the technical field of quality detection, and discloses a method for determining the content of five components of liver-soothing tablets based on a double-label multi-measurement method, and the method comprises the following steps: S1, extracting a test solution; s2, preparing a reference substance solution; s3, preparing each negative control solution; s4, determining chromatographic conditions; s5, constructing a retention time prediction model by using a double-marking linear correction method, taking the standard retention time of paeoniflorin and hesperidin as a horizontal coordinate, taking the actual retention time of paeoniflorin and hesperidin in the chromatographic column as a vertical coordinate, and substituting the standard retention time of agarotetrol, naringin and neohesperidin into the equation to calculate and obtain predicted retention time respectively; s6, determining the contents of paeoniflorin, agarotetrol, naringin, hesperidin and neohesperidin in the liver soothing tablet sample by adopting a double-label multi-determination method; according to the method, the contents of five components, namely paeoniflorin, agarotetrol, naringin, hesperidin and neohesperidin, in the liver-soothing tablet are simultaneously determined by a double-label multi-measurement method, the accuracy is high, and the cost is low.

The invention discloses a quality detection method of a gout clearing mixture for treating hyperuricemia, which adopts a separation analysis technology of ultra-high performance liquid chromatography to detect eight chemical components in the gout clearing mixture. Comprising the following components: 3-O-feruloylquinic acid, 5-O-caffeoylshikimic acid, paeoniflorin, polydatin, neoastilbin, astilbin, berberine hydrochloride and emodin-8-beta-D-glucopyranoside. According to the method, eight chemical components of six active components in the gout clearing mixture are quantitatively analyzed at the same time, and the method has the advantages of being easy and convenient to operate, good in stability and easy to popularize. The invention can provide a comprehensive and reliable quality control basis for the gout clearing mixture, and has important significance for ensuring the clinical curative effect of the gout clearing mixture.

The invention provides application of paeoniflorin in preparation of a medicine for inhibiting diabetic retinal vascular hyperplasia. The active ingredients of the medicine are one or more of paeoniflorin, a hydrate of paeoniflorin, pharmaceutically acceptable salt of paeoniflorin, a tautomer of paeoniflorin, a stereoisomer of paeoniflorin and a precursor compound of paeoniflorin. Paeoniflorin has potential to be developed into drugs, health care products or functional food for treating diabetic retinopathy.

The invention provides a preparation method of traditional Chinese medicine hydrogel for treating psoriasis, the hydrogel preparation is prepared from the following raw material medicines in parts by weight: 20-40 parts of PVA, 60-80 parts of borax, 20-40 parts of glycyrrhizic acid, 8-12 parts of paeoniflorin and 2-3 parts of glycerin, the hydrogel has the advantages of injectability, self-healing, stretchability and the like, can meet the requirements of skin dressing, can be tightly attached to the skin, and has a good application prospect. The separation is easy. Animal experiments prove that after being loaded with paeoniflorin, the gel has the curative effect of treating mild and moderate psoriasis, the treatment effect is remarkable, and the gel has a wide market application prospect.

The invention relates to a method for extracting paeonol and paeoniflorin from fresh radix paeoniae rubra. The method comprises the following specific steps: washing and mincing the fresh radix paeoniae rubra, extracting with diethyl ether, performing alcohol extraction and water extraction, filtering, concentrating, performing alcohol precipitation, crystallizing and recrystallizing to obtain pure paeonol and paeoniflorin products. According to the method disclosed by the invention, diethyl ether extraction is adopted, so that not only is the enzyme activity of the fresh peony accelerated to be damaged, but also the risk that paeonol is oxidized is reduced, the leaching speed of paeonol is increased, the utilization efficiency of the fresh peony is greatly improved, the drying step of the fresh peony is effectively reduced, and the production energy consumption during extraction processing is saved; the dissolution and volatilization characteristics of paeonol are fully considered, diethyl ether removal and paeonol volatilization are carried out by utilizing different temperature gradients, and refining is carried out to obtain a pure paeonol product.

The invention discloses a preparation process of peony seed extract with high paeoniflorin and resveratrol content, and belongs to the technical field of paeoniflorin and resveratrol extraction. The technical problem to be solved is to improve the content of resveratrol and paeoniflorin in the peony seed extract. According to the technical scheme, peony seeds are smashed, soaked, subjected to alcohol extraction, filtered, concentrated and dried; a solvent for alcohol extraction is an ethanol solution with the volume fraction of 70-80%, and the solid-liquid ratio of alcohol extraction is 1g: (15-20) g.

The invention discloses a composition for inhibiting bacteria, resisting inflammation, relieving inflammation and repairing sensitive skin. The composition comprises the following components in parts by weight: 1-5 parts of a sophora flavescens extract; 2-15 parts of a radix scutellariae extract; 5-10 parts of a white peony root extract; 0.2 to 5 parts of a grapefruit extract; and 1-5 parts of pomegranate extract. The composition prepared from the radix sophorae flavescentis extract, the radix scutellariae extract, the radix paeoniae alba extract, the grapefruit extract and the pomegranate extract takes sophocarpidine, baicalin, paeoniflorin, naringin and punicalagin as main functional components to play a synergistic effect, so that the composition has very remarkable effects of inhibiting bacteria, resisting inflammation, relieving inflammation and repairing sensitive skin.

The invention provides a pharmaceutical composition for treating prostatitis and a preparation method thereof. The pharmaceutical composition is prepared from the following raw materials in parts by weight: 20 to 60 parts of palmatine, 20 to 60 parts of paeoniflorin, 10 to 30 parts of formononetin, 20 to 60 parts of astilbin and 10 to 40 parts of stigmasterol. In-vitro cell tests and in-vivo animal experiments prove that the pharmaceutical composition has the effect of relieving the chronic prostatitis reaction.

The invention discloses a platelet increasing capsule characteristic chromatogram and a construction method and application thereof, and belongs to the technical field of quality control of traditional Chinese medicine compound preparations. According to the method, 13 control components including gallic acid, catechin, paeoniflorin, forsythiaside B, benzoic acid, astragalus smicus glycoside, forsythin, arctiin, liquiritigenin, benzoyl paeoniflorin, paeonol, arctigenin and indirubin are selected, a high performance liquid chromatography-mass spectrometry technology is applied, multiple batches of preparation samples are analyzed, and the content of the components in the preparation samples is determined. And establishing a characteristic spectrum of the chemical components. Compared with a traditional method, the technology has good precision and reproducibility, the data result is accurate and reliable, meanwhile, the technology can identify the 13 components at the same time under the same chromatographic condition, the analysis efficiency is remarkably improved, synchronous monitoring of multiple effective components is achieved, and the consistency and stability of the preparation quality can be guaranteed.

The invention discloses a novel application of total glucosides of paeony in preparation of a medicine for treating triple negative breast cancer. In-vitro and in-vivo experiments prove that the total glucosides of paeony can remarkably inhibit proliferation, migration and invasion of triple negative breast cancer cells (MDA-MB-231 and MDA-MB-468), reverse an epithelial-mesenchymal transition process and induce cell apoptosis by inducing mitochondrial injury, inhibiting oxidative phosphorylation and activating a mitochondrial apoptosis pathway. In an MDA-MB-231 cell xenotransplantation tumor nude mouse model, the total glucosides of paeony can effectively inhibit tumor growth. Therefore, the total glucosides of paeony can be used for preparing the medicine for treating the triple negative breast cancer, and a new treatment choice is provided for clinic.

The application provides a method for establishing HPLC fingerprint of Changnyle capsule (I), which is characterized in that 16 active components including gastrodin, danshensu, quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiopicroside, 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, puerarin apioside, paeoniflorin, daidzin, rosmarinic acid, shikonin, isoquercitrin, ononin, daidzein, salvianolic acid B and calycosin are used as reference substances, HPLC is adopted, methanol-acetonitrile-0.5vol% phosphoric acid aqueous solution is used as a mobile phase, different batches of samples are detected, and finally the fingerprint of Changnyle capsule (I) is obtained. The fingerprint can comprehensively represent the main components in Changnyle capsule (I), thereby being beneficial to the quality control of Changnyle capsule (I).

This invention relates to a highly active effective fraction of the Epiglottis-Clearing Decoction and its application in the preparation of antitussive drug compositions, belonging to the field of traditional Chinese medicine extract technology. The effective fraction contains hydroxysaffron yellow A, paeoniflorin, naringin, and neohesperidin, with the total content of these four components not less than 20% of the total mass of the effective fraction. The effective fraction of this invention is prepared by purification with macroporous resin. Compared with the freeze-dried powder of the traditional Epiglottis-Clearing Decoction, the total content of the four active ingredients is increased from 3.32% to 20.87%, achieving a more than 6-fold high-efficiency enrichment. Animal pharmacodynamic experiments show that the antitussive effect of a low dose (275.5 mg / kg) of this effective fraction is comparable to that of the freeze-dried powder of the original decoction (1928 mg / kg), reducing the dosage to about 1 / 7 of the original formula, demonstrating a significant "synergistic effect with reduced dosage" technical effect. The effective active ingredients provided by this invention have a clear content and stable quality, and can be made into modern preparations such as tablets, capsules or granules, solving the problems of large volume, inaccurate dosage and poor patient compliance of traditional decoctions.

The application discloses a kind of self-assembly hydrogel, preparation method and application, belong to material field.The application claims to protect a kind of self-assembly hydrogel, it is formed by glycyrrhizic acid, five gallate glucose and paeonol by molecular self-assembly;Wherein, the mass ratio of glycyrrhizic acid and five gallate glucose, paeonol is (10~60) :(1~8) :(2~8).The self-assembly hydrogel provided by the application is suitable for skin, raw material is natural, biocompatibility is good, mechanical and storage stability is excellent, can release paeonol and the transdermal effect is good, three components synergistically improve atopic dermatitis and other skin inflammation, and clinical value is high.In addition, the preparation process of the self-assembly hydrogel of the application is simple, can be prepared using conventional one-pot method, easy to produce.Therefore, the self-assembly hydrogel of the application has the prospect of developing into paeonol sustained-release delivery system, paeonol transdermal sustained-release preparation or skin inflammation treatment drug.

The application provides a pharmaceutical composition for treating rheumatoid arthritis, which is composed of paeoniflorin, glycyrrhizin, glycyrrhizic acid and glycyrrhetic acid in a weight ratio of (4.5-7.5):(0.4-1.8):(1.8-4.3):(22-40). The pharmaceutical composition can significantly inhibit the proliferation of fibroblast-like synoviocytes, reduce the secretion of inflammation-related factors (TNF-alpha, IL-1beta, IL-6 and COX-2), regulate the expression of TNF-alpha / NF-kappaB signal pathway-related proteins, and has a significant curative effect on rheumatoid arthritis.

The invention provides a method for preparing paeoniflorin and albiflorin by utilizing supercritical chromatography, and belongs to the field of natural products. The invention provides a method for preparing paeoniflorin and albiflorin by using supercritical chromatography, which comprises the following steps: carrying out supercritical chromatography analysis on a solution of a sample to obtain a chromatogram of the sample; if the chromatogram shows that the sample contains paeoniflorin and albiflorin, carrying out supercritical chromatographic separation on the solution of the sample to obtain pure paeoniflorin and pure albiflorin; a chromatographic column used for the supercritical chromatographic analysis and the supercritical chromatographic separation is silica. The method is simple, convenient and efficient, simple in pretreatment, high in analysis and separation speed, small in used solvent amount, easy to recover and reusable, time, manpower and material resources are saved, the purity of paeoniflorin and albiflorin is improved by selecting silica as a chromatographic column, the single sample loading amount is large, and high-purity, rapid and large-scale preparation of paeoniflorin and albiflorin can be achieved.

The present invention discloses a method for simultaneously detecting seven effective ingredients in Yangyin Qingfei Pills, and belongs to the technical field of chemical detection. The method for simultaneously detecting seven effective ingredients in Yangyin Qingfei Pills described in the present invention comprises the following steps: injecting the Yangyin Qingfei Pill sample solution into a liquid chromatograph, and using a segmented wavelength conversion method to perform chromatographic detection to achieve qualitative and quantitative detection of the seven effective ingredients. The present invention uses the UPLC segmented wavelength conversion method to achieve simultaneous determination of the contents of harpagine, paeoniflorin, liquiritin, verbascoside, harpagine, paeonol and glycyrrhizic acid in Yangyin Qingfei Pills, and all seven effective ingredients can be well separated without interference from other ingredients. Moreover, each component has a good linear relationship within its own linear range, and the sample recovery rate is between 95% and 105%. The detection method of the present invention is simple, accurate and effective, and can be used for quality control and overall quality evaluation of Yangyin Qingfei Pills.

The invention provides application of a paeoniflorin photoaffinity probe in screening key target protein of paeoniflorin, and relates to the technical field of chemical proteomics. The paeoniflorin photoaffinity probe has a structure as shown in a formula I, and the paeoniflorin key target proteins are 40S ribosomal protein S3 and statin 2. The paeoniflorin photoaffinity probe is applied to screening of the paeoniflorin key target proteins, so that two target proteins combined with paeoniflorin in liver cells are successfully identified; the two target proteins are respectively 40S ribosomal protein S3 (RPS3) and statin 2 (PHB2), and the two target proteins participate in the protection effect of paeoniflorin on the liver, and have the key effects of oxidation resistance, inflammation resistance, immunoregulation and the like in liver diseases.

The invention belongs to the technical field of biology and new medicine, and discloses a preparation method of acoustic resonance driven glycyrrhizic acid nano-composite responsive gel, glycyrrhizic acid is used as a drug carrying system, berberine, ursolic acid, baicalein, luteolinin, curcumin, baicalin or ginsenoside Rh2 is used as a water-insoluble natural small molecule drug, and the acoustic resonance driven glycyrrhizic acid nano-composite responsive gel is prepared. Glycyrrhizic acid is an amphiphilic compound composed of one molecule of glycyrrhetinic acid and two molecules of glucuronic acid, a hydrophobic cavity can be formed in a water environment to wrap the water-insoluble natural small molecule medicine, a glycyrrhizic acid nano-composite solution is formed, and meanwhile the solubility of the water-insoluble natural small molecule medicine such as baicalein is improved.

The present invention provides a method for detecting the content of anti-inflammatory active ingredients in a rhubarb and peony preparation. The method utilizes specific solvents and chromatographic conditions, and employs multi-wavelength switching technology to simultaneously and quantitatively detect nine anti-inflammatory active ingredients in rhubarb and peony granules. The method is simple to operate, has good stability, high accuracy, short detection time, high efficiency, and low detection cost. The method calculates the content of the main active ingredients in the rhubarb and peony preparation, including gallic acid, amygdalin, paeoniflorin, paeonol, aloe-emodin, rhein, emodin, chrysophanol, and physcion, by calculating the relative correction factor f. This method provides a more comprehensive method for quality control of rhubarb and peony preparations.

The present invention provides a method for constructing a fingerprint of a traditional Chinese medicine preparation for treating eczema, comprising the following steps: taking gallic acid, gentiopicroside, geniposide, paeoniflorin, ammonium glycyrrhizate, salvianolic acid B, baicalin, baicalein and wogonin reference substances to prepare a mixed reference substance solution; mixing the traditional Chinese medicine preparation for treating eczema with an organic solvent to prepare a test solution; the organic solvent is selected from one or more of methanol aqueous solution, ethanol aqueous solution and anhydrous ethanol; the mixed reference substance solution and the test solution are respectively subjected to high performance liquid chromatography determination; wherein the conditions adopted by the high performance liquid chromatography include: mobile phase A is selected from one or more of methanol and acetonitrile, and mobile phase B is selected from one or more of phosphoric acid aqueous solution, acetic acid aqueous solution and formic acid aqueous solution; and a gradient elution program is adopted.

The invention discloses a method for determining chemical components in a medicine for treating vitiligo, which relates to the technical field of medicine analysis, and is characterized in that ultra-high performance liquid chromatography-mass spectrometry is adopted to determine the content of chemical components in a Bailing tablet: psoralen, isopsoralen, astragaloside, quercetin, polysaccharide and amino acid. The determination method comprises the following steps: respectively injecting a reference solution and a test solution into a liquid chromatograph for determination to obtain a chromatogram, and calculating the content of paeoniflorin in a test sample by adopting an external standard method, chromatographic conditions of a chromatogram obtained by adopting a liquid chromatograph are as follows: octadecylsilane chemically bonded silica is taken as a filling agent. According to the method for determining the chemical components in the medicine for treating leucoderma, the established detection method is verified, and the result shows that the detection method is easy and convenient to operate, accurate in result, high in specificity and good in reproducibility.

The invention discloses paeoniflorin serving as an SIRT1 agonist and application of the paeoniflorin. Test results prove that paeoniflorin can be stably combined with SIRT1 at an agonist site so as to promote the activity of deacetylase SIRT1. The deacetylase SIRT1 agonist disclosed by the invention has a good activation effect on a deacetylation reaction of SIRT1 and a p53 substrate on a molecular level. The application proves that paeoniflorin can significantly improve nano-zinc oxide-induced mouse kidney injury in vivo, and can be used as a potential medicine for treating nano-zinc oxide-induced kidney injury by improving renal dysfunction, activating kidney SIRT1 and increasing antioxidant enzyme activity. The new function of paeoniflorin found in the invention provides a new strategy for research and development of SIRT1 agonist drugs, and provides a new direction for development of nano-zinc oxide protective agents.

The application provides a method for detecting the pharmacodynamic components of a traditional Chinese medicine composition by UPLC-MS / MS. The method can identify 14 pharmacodynamic components of the traditional Chinese medicine composition, including saikosaponin A, saikosaponin D, naringin, paeoniflorin, hesperidin, chicoric acid, chlorogenic acid, salvianolic acid B, zephyranthine, kaempferitrin, and codariusrhizome glycoside, amargin, hyperoside and rutin, thereby providing a reference for evaluating the intrinsic quality of the traditional Chinese medicine composition.

The application discloses a freeze-dried mask based on Yuyong Powder and a preparation method thereof, and the preparation method comprises the following steps: after raw materials of the Yuyong Powder are crushed at low temperature, the raw materials are extracted at low temperature by using a composite solvent, and then the extracted solution is refined by flocculation clarification, centrifugation and microfiltration to obtain a refined extraction solution; the refined extraction solution is mixed with a humectant and a freeze-drying protective agent, and then is injected into a mask support, and gradient temperature control freeze-drying is carried out; finally, the freeze-dried mask is sealed and packaged under sterile conditions by using nitrogen. Through the whole low-temperature process and the synergistic effect of the composite freeze-drying protective agent, the heat-sensitive whitening components such as paeoniflorin, salvianolic acid and glabridin in the Yuyong Powder are effectively retained; impurities are removed through three-stage refining and purification, so that the irritability of the product is reduced; through freeze-drying and high-barrier packaging, the product can be stored at room temperature for a long time. The obtained freeze-dried mask can be quickly reconstituted when meeting water, has a refreshing skin feeling, and has remarkable effects of removing yellow and brown spots and whitening, and has the advantages of high activity, high stability, high convenience and high safety.

The invention belongs to the technical field of medicines, and particularly relates to an active ingredient composition for treating xerophthalmia and muscae volitantes as well as a preparation method and application of the active ingredient composition. The composition is prepared from lycium barbarum polysaccharide, zeaxanthine, apigenin, luteolin, zinc gluconate, ophiopogon japonicus polysaccharide, ophiopogonin B, paeoniflorin and gallic acid according to a specific weight part ratio, all the active ingredients have a synergistic effect, the ocular surface microenvironment is improved, the vitreous opacity progress is inhibited, eye tissue damage is repaired, and the eye quality is improved. The synergistic treatment effect on the xerophthalmia and the muscae volitantes is achieved, and the symptoms such as eye dryness, eye acerbity, lacrimal secretion insufficiency, black shadow fluttering and blurred vision can be effectively relieved.

The application belongs to the field of natural product extraction and cosmetic raw materials, and discloses a preparation method and application of a composition containing asiaticoside-paeoniflorin, which comprises two key and functionally different stages: ① extraction stage: using asiaticoside as an extraction aid and using propylene glycol as a complex medium, efficiently dissolving and forming an "asiaticoside-paeoniflorin" supramolecular complex; ② complexing and stabilizing stage: using glycerol as a stabilizer to maintain the long-term kinetic stability of the formed supramolecular complex and prevent its dissociation and aggregation. The method solves the technical problem that the extraction rate and stability of paeoniflorin are difficult to be considered simultaneously in the field by functional division of extraction and stabilization, and the composition containing asiaticoside-paeoniflorin obtained finally has excellent soothing performance, not only rapid effect, but also persistent and stable soothing protection effect, which can effectively resist and repair the influence of external stimulation on the skin.

The invention discloses a traditional Chinese medicine monomer targeting MEK2 protein and application of the traditional Chinese medicine monomer. The invention provides an application of a substance for activating an MEK2 / ERK pathway in preparation of a product with any one of the following functions: A1) treating acute hypoxia lung injury; and A2) improving the acute hypoxia lung injury. Experiments prove that paeoniflorin is a natural compound with remarkable treatment potential, has important significance in treatment of acute hypoxia lung injury, and is worthy of further deep research and clinical application exploration.