14 results about "Paeoniflorin" patented technology

The application provides a method for establishing HPLC fingerprint of Changnyle capsule (I), which is characterized in that 16 active components including gastrodin, danshensu, quercetin-3-O-beta-D-glucose-7-O-beta-D-gentiopicroside, 3'-hydroxy puerarin, puerarin, 3'-methoxy puerarin, puerarin apioside, paeoniflorin, daidzin, rosmarinic acid, shikonin, isoquercitrin, ononin, daidzein, salvianolic acid B and calycosin are used as reference substances, HPLC is adopted, methanol-acetonitrile-0.5vol% phosphoric acid aqueous solution is used as a mobile phase, different batches of samples are detected, and finally the fingerprint of Changnyle capsule (I) is obtained. The fingerprint can comprehensively represent the main components in Changnyle capsule (I), thereby being beneficial to the quality control of Changnyle capsule (I).

This invention relates to a highly active effective fraction of the Epiglottis-Clearing Decoction and its application in the preparation of antitussive drug compositions, belonging to the field of traditional Chinese medicine extract technology. The effective fraction contains hydroxysaffron yellow A, paeoniflorin, naringin, and neohesperidin, with the total content of these four components not less than 20% of the total mass of the effective fraction. The effective fraction of this invention is prepared by purification with macroporous resin. Compared with the freeze-dried powder of the traditional Epiglottis-Clearing Decoction, the total content of the four active ingredients is increased from 3.32% to 20.87%, achieving a more than 6-fold high-efficiency enrichment. Animal pharmacodynamic experiments show that the antitussive effect of a low dose (275.5 mg / kg) of this effective fraction is comparable to that of the freeze-dried powder of the original decoction (1928 mg / kg), reducing the dosage to about 1 / 7 of the original formula, demonstrating a significant "synergistic effect with reduced dosage" technical effect. The effective active ingredients provided by this invention have a clear content and stable quality, and can be made into modern preparations such as tablets, capsules or granules, solving the problems of large volume, inaccurate dosage and poor patient compliance of traditional decoctions.

The application discloses a kind of self-assembly hydrogel, preparation method and application, belong to material field.The application claims to protect a kind of self-assembly hydrogel, it is formed by glycyrrhizic acid, five gallate glucose and paeonol by molecular self-assembly;Wherein, the mass ratio of glycyrrhizic acid and five gallate glucose, paeonol is (10~60) :(1~8) :(2~8).The self-assembly hydrogel provided by the application is suitable for skin, raw material is natural, biocompatibility is good, mechanical and storage stability is excellent, can release paeonol and the transdermal effect is good, three components synergistically improve atopic dermatitis and other skin inflammation, and clinical value is high.In addition, the preparation process of the self-assembly hydrogel of the application is simple, can be prepared using conventional one-pot method, easy to produce.Therefore, the self-assembly hydrogel of the application has the prospect of developing into paeonol sustained-release delivery system, paeonol transdermal sustained-release preparation or skin inflammation treatment drug.

The application discloses a freeze-dried mask based on Yuyong Powder and a preparation method thereof, and the preparation method comprises the following steps: after raw materials of the Yuyong Powder are crushed at low temperature, the raw materials are extracted at low temperature by using a composite solvent, and then the extracted solution is refined by flocculation clarification, centrifugation and microfiltration to obtain a refined extraction solution; the refined extraction solution is mixed with a humectant and a freeze-drying protective agent, and then is injected into a mask support, and gradient temperature control freeze-drying is carried out; finally, the freeze-dried mask is sealed and packaged under sterile conditions by using nitrogen. Through the whole low-temperature process and the synergistic effect of the composite freeze-drying protective agent, the heat-sensitive whitening components such as paeoniflorin, salvianolic acid and glabridin in the Yuyong Powder are effectively retained; impurities are removed through three-stage refining and purification, so that the irritability of the product is reduced; through freeze-drying and high-barrier packaging, the product can be stored at room temperature for a long time. The obtained freeze-dried mask can be quickly reconstituted when meeting water, has a refreshing skin feeling, and has remarkable effects of removing yellow and brown spots and whitening, and has the advantages of high activity, high stability, high convenience and high safety.

The application belongs to the field of natural product extraction and cosmetic raw materials, and discloses a preparation method and application of a composition containing asiaticoside-paeoniflorin, which comprises two key and functionally different stages: ① extraction stage: using asiaticoside as an extraction aid and using propylene glycol as a complex medium, efficiently dissolving and forming an "asiaticoside-paeoniflorin" supramolecular complex; ② complexing and stabilizing stage: using glycerol as a stabilizer to maintain the long-term kinetic stability of the formed supramolecular complex and prevent its dissociation and aggregation. The method solves the technical problem that the extraction rate and stability of paeoniflorin are difficult to be considered simultaneously in the field by functional division of extraction and stabilization, and the composition containing asiaticoside-paeoniflorin obtained finally has excellent soothing performance, not only rapid effect, but also persistent and stable soothing protection effect, which can effectively resist and repair the influence of external stimulation on the skin.

The present application relates to the field of pharmacology, in particular to the use of paeoniflorin-6'-O-benzene sulfonate for improving acinar atrophy, maintaining the stability of gland functional units, and repairing gland secretion function. The paeoniflorin-6'-O-benzene sulfonate can effectively improve the acinar atrophy of diabetic animals, and the effect is significantly better than that of paeoniflorin, and can be used for preparing a medicine for treating xeroma.

This invention relates to the field of traditional Chinese medicine preparation technology, and discloses a fingerprint-standardized traditional Chinese medicine composition for relieving myopia, comprising: the composition is obtained by extracting Gastrodia elata, Paeonia lactiflora and Salvia miltiorrhiza, and contains gastrodin, paeoniflorin and salvianolic acid B; the composition contains micelles formed by endogenous components, with gastrodin, paeoniflorin and salvianolic acid B distributed in the hydrophobic centers inside the micelles; the composition induces micelle interface contraction by adjusting the polarity of the extract and the temperature gradient, thereby sealing the active components within the micelles to cut off their thermodynamic rearrangement pathways, and the fingerprint spectrum similarity of its common characteristic peaks is not less than 0.90. This invention utilizes the micelle interface locking mechanism to fix the spatial distribution of active components, solves the problem of ratio dispersion caused by reverse analysis of active ingredients in the extraction stage, and ensures that the composition has a constant biological activity output in relieving ciliary muscle spasm and improving retinal microcirculation.

The present application provides a kind of pharmaceutical composition for vulvar sclerosing lichen and its preparation method, the pharmaceutical composition includes active ingredient and pharmaceutically acceptable vulva local administration adjuvant, active ingredient is made of 0.005-0.02 parts of mometasone furoate, 0.05-0.3 parts of calcineurin inhibitor, 0.5-2 parts of paeoniflorin, 0.8-3 parts of asiaticoside, 0.3-1.5 parts of recombinant collagen, 0.2-1 part of ginkgolide B.The present application is aimed at the core pathogenesis of disease, constructs multi-target whole course of disease synergistic therapy system, can realize anti-inflammatory regulation, anti-fibrosis, basement membrane repair, microcirculation improvement simultaneously, low-dose strong effect, greatly reduce side effect and recurrence rate;Liposome targeted delivery technology is used, the stability of active ingredient is guaranteed, process controllable adaptation industrialization production, and clinical application prospect is wide.

This invention belongs to the field of natural product extraction and cosmetic raw materials, and discloses a method for preparing a composition containing asiaticoside-paeoniflorin and its application. The preparation method includes two key and functionally distinct stages: ① Extraction stage: using asiaticoside as an extraction aid and propylene glycol as a composite medium, efficiently dissolving and forming a supramolecular complex of "asiaticoside-paeoniflorin"; ② Complex stabilization stage: using glycerol as a stabilizer to maintain the long-term kinetic stability of the formed supramolecular complex and prevent its dissociation and aggregation. This method, through the functional division of labor between extraction and stabilization, synergistically solves the long-standing technical problem in the field of balancing the extraction rate and stability of paeoniflorin. Furthermore, the composition containing asiaticoside-paeoniflorin obtained by this invention possesses excellent soothing properties, not only acting rapidly but also providing long-lasting and stable soothing protection, effectively combating and repairing the effects of external stimuli on the skin.

This invention proposes a thin-layer chromatography (TLC) method for distinguishing between honeycomb-based traditional Chinese medicine (TCM) formula granules and stir-fried honeycomb-based TCM formula granules, as well as a method for preparing the honeycomb-based TCM formula granules and stir-fried honeycomb-based TCM formula granules. The identification method includes: after spotting, the TLC plate is first placed in a mixture of petroleum ether and diethyl ether at a volume ratio of 2:1 for development, removed, and dried. Then, the dried TLC plate is placed in a mixture of toluene, acetone, and formic acid at a volume ratio of 5:4:1 for development, removed, and dried. Finally, it is examined under ultraviolet light. The stir-fried honeycomb-based TCM formula granules show identification spots at Rf values ​​of 0.540-0.660 and 0.590-0.722, respectively, while the honeycomb-based TCM formula granules show no spots. The TLC method provided by this invention can quickly and effectively identify honeycomb-based TCM formula granules and stir-fried honeycomb-based TCM formula granules that have lost their medicinal slice form. Moreover, this method has strong specificity, good stability, high precision, convenience, and is easy to master.

The application discloses an anti-fatigue plant source nutritional supplement containing total paeony glycosides and a preparation method thereof. The nutritional supplement comprises a total paeony glycoside derivative modified by enzymatic selective acylation, vitamins and minerals, and a compound of acanthopanax senticosus extract, astragalus extract, medlar extract and grape seed extract. The total paeony glycoside derivative is selectively modified by introducing a C4-C8 medium-chain fatty acid acyl group to the C-6' hydroxyl group of paeonoside. The application significantly improves the fat solubility and membrane permeability of the total paeony glycoside by using a green and mild enzymatic acylation technology, so that the apparent permeability coefficient is increased by 8-12 times. The nutritional supplement prolongs the weight-bearing swimming time of mice by 92.8%, significantly reduces the serum urea nitrogen and blood lactic acid levels, increases the blood lactic acid elimination ratio to 37.8%, activates the AMPK-PGC1 alpha energy metabolism pathway and adjusts the central nervous transmitter 5-HT / DA ratio, and has a synergistic anti-fatigue effect.

The application provides a preparation method of paeonol glycoside for inhibiting GM-CSF + The application belongs to the technical field of biological medicine, and relates to application of Th cell differentiation in a medicine for preventing and / or treating rheumatoid arthritis (RA). + Abnormal activation of Th cells is obviously earlier than appearance of clinical symptoms of RA in a time axis, is an early key initiating factor for driving occurrence of the RA disease, and is a core target point for implementing an early blocking strategy. + Further, it is found that paeonol glycoside (Alb) can block a gamma c / JAK3 / STAT5 signal path by targeting and combining with an interleukin-2 receptor gamma chain, down-regulate CSF2 gene transcription and GM-CSF protein expression, thereby inhibiting differentiation of Th cells, and finally achieving an effect of preventing occurrence of CIA arthritis and not rebounding after drug withdrawal. The application provides a new intervention medicine which is high in safety, suitable for long-term use, can effectively regulate immune abnormalities in a pre-RA stage, prevents rheumatoid arthritis, and has a wide application prospect.

A kind of overall quality control method of compound eucommia ulmacea waist capsule, including: psoralen and isopsoralen content determination: with hydrochloric acid-methanol solution as extraction solvent, heating reflux extraction, with methanol-phosphoric acid aqueous solution as mobile phase isocratic elution;Macon glycoside, paeoniflorin and paeonol glycoside content determination: with 50% ethanol ultrasonic extraction, with acetonitrile-0.1% phosphoric acid solution as mobile phase gradient elution;HPLC fingerprint is established: 21 common peaks are calibrated, 6 characteristic peaks are identified, and the similarity is not less than 0.992;Thin layer chromatography identification: establish white peony root and cornus officinalis synchronous identification, ephedra identification and radix rehmanniae praeparata identification method.The present application is based on the identification of 92 kinds of compounds by LC-MS technology, and a multi-dimensional quality control system combining multi-component quantitative, fingerprint overall evaluation and exclusive identification is constructed, which overcomes the shortcomings of single existing standard index and lack of overall evaluation, and can comprehensively and accurately control the quality of compound eucommia ulmacea waist capsule.

This invention relates to a method for detecting common components of peony bark that can be transferred to Liuwei Dihuang oral liquid. The method comprises two parts: First, using thin-layer chromatography (TLC) to detect in parallel samples 1 (test solution 1), peony bark solution 1, paeonol solution 1, and negative sample solution 1, to obtain spots of at least two peony bark components that can be transferred to Liuwei Dihuang oral liquid; second, using high-performance liquid chromatography (HPLC) to detect sample solution 2, peony bark solution 2, paeonol solution 2, paeoniflorin solution, and negative sample solution 2, to obtain fingerprint chromatograms of common characteristic peaks of at least six peony bark components that can be transferred to Liuwei Dihuang oral liquid. The TLC and HPLC fingerprinting techniques of this invention have advantages such as high efficiency, accuracy, high specificity, high separation, and simple operation, providing a reliable regulatory technology for combating illegal activities related to the manufacture and sale of counterfeit and substandard Liuwei Dihuang oral liquid.