194 results about "Supercritical fluid chromatography" patented technology

The invention discloses a preparing method of total sweet chrysanthemum glycosides and total chromocor in sweet leaf chrysanthemum, which is characterized by the following: comprising sweet chrysanthemum glycosides, stevi primary glycosides, labroid glycosides A, B, C, D, E, F, duacl glycosides A and so on in total sweet chrysanthemum glycosides; comprising cyanidenon, meletin, cyanidenon-7-0-beta-D glycosides, celery element-7-0-beta-D-glycosides, quercetin, meletin-3-0-beta-D-arabinoside, meletin-3-0-[4-0-trans-coffe acyl-alpha-L-isodulcitol-(1-6)-beta-D-arabinoside] and so on; choosing one or several methods from solvent extraction, solvent extraction process, macroreticular absorption resin method, column chromatography, supercritical fluid chromatography, liquid-liquid counter-current partition chromatography and so on; extracting the total chromocor; setting content of sweet chrysanthemum glycosides element in total sweet chrysanthemum glycosides at 5-100%; counting 5-100% of all sweet chrysanthemum glycosides content with sweet chrysanthemum glycosides and labroid glycosides; counting 5-100% of chromocor element in sweet leaf chrysanthemum total chromocor; counting 5-100% of all total chromocor content with cyanidenon-7-0-beta-D glycosides, quercetin and meletin-3-0-[4-0-trans-coffe acyl-alpha-L-isodulcitol-(1-6)-beta-D-arabinoside].

The invention discloses a peony total glycosides extract from traditional medicine white peony root or red peony root and preparing method, which is characterized by the following: comprising peony glycosides, white peony glycosides, peony new glycosides, peony inner ester A, B, C, oxidation peony glycosides, benzoyl peony glycosides, derivant and so on; choosing one or several methods from solvent extraction, solvent extraction process, macroreticular absorption resin method, column chromatography, supercritical fluid chromatography, liquid-liquid counter-current partition chromatography and so on; producing extract; setting sum of various terpene glycosides percentage content at 5-100%(w/w); setting content of peony glycosides at 5-100%(w/w).

The invention discloses a total chromocor extract from traditional medicine citrus fruit and preparing method, which is characterized by the following: comprising pomelo ped glycosides, new orange peel glycosides, orange peel glycosides, pomelo ped rutin, derivant and so on; choosing one or several methods from solvent extraction, solvent extraction process, macroreticular absorption resin method, lead salt precipitation method, column chromatography, supercritical fluid chromatography, liquid-liquid counter-current partition chromatography and so on; producing extract; setting percentage content of various chromocor element at 5-100%(w/w) in the extract; counting 5-100%(w/w) of pomelo ped glycosides, new orange peel glycosides, orange peel glycosides in total chromocor content.

The present invention provides a method for the purification of a prostaglandin by supercritical fluid chromatography, said method comprising the use of a stationary phase and a mobile phase comprising carbon dioxide, provided that when the stationary phase is unmodified silica gel, the prostaglandin is not luprostiol. The invention also provides prostaglandins obtainable by the method.

An apparatus and process to effectively create an active drain for high and low pressure gas-liquid separator. The invention accomplishes a deterministic drainage rate of variable volume and viscosity liquid fractions from the separator. The mechanisms and methods thereof create a flow path from the separator that dramatically favors controlled liquid flow over a wide range of flow rates and viscosities while restricting gas flow. The invention is a superior method of drainage over conventional systems that employ pressure, vacuum, isolation valves and/or passive flow restrictors to achieve drainage of the separators. The embodiments are primarily directed to the fields of preparative supercritical fluid chromatography (SFC) and supercritical fluid extraction (SFE). An advantage of one embodiment allows the economical conversion of typical HPLC systems to state of-the-art supercritical fluid chromatography (SFC) systems with minimal modification to system components.

The invention discloses an industrially-applicable method for preparing a polymer foamed material by using supercritical fluid technology, which comprises the following steps: 1) forming a polymer raw material to be foamed into raw blanks by melting, blending and cooling; 2) placing the raw blanks in a container, charging supercritical fluid into the container, and allowing the raw blanks to swell and the supercritical fluid to permeate into the raw blanks; and 3) initiating nucleation and foaming by controlling pressure relief rate and obtaining the closed micro pore polymer foamed material with uniform pore diameter and adjustable pore density. According to the invention, the process is simple, the production process is clean and environment-friendly, the efficiency is high, and the prepared foamed material has high mechanical performance and can be used in fields of transportation vehicles, building materials, packages and the like.

The present invention pertains to apparatus and methods for introduction of solid precursors and reactants into a supercritical fluid reactor. Solids are dissolved in supercritical fluid solvents in generator apparatus separate from the supercritical fluid reactor. Such apparatus preferably generate saturated solutions of solid precursors via recirculation of supercritical fluids through a vessel containing the solid precursors. Supercritical solutions of the solids are introduced into the reactor, which itself is charged with a supercritical fluid. Supercritical conditions are maintained during the delivery of the dissolved precursor to the reactor. Recirculation of supercritical precursor solutions through the reactor may or may not be implemented in methods of the invention. Methods of the invention are particularly well suited for integrated circuit fabrication, where films are deposited on wafers under supercritical conditions.

The invention provides an apparatus for supercritical fluid chromatography. The apparatus comprises a binary pump; an autosampler; a sampling valve; a first and second port switching valve; a first and second manifold; two or more channels, each having a check valve assembly, a separation column and one or more detectors operatively connected thereon; and a backpressure regulator. The apparatus also includes computer software and hardware to control distribution of fluid through the apparatus, including switching between a multi-channel mode or a single channel ode; 2) analyze data collected by the one or more detectors; and 3) optimize separation of analytes by controlling solvent combinations, concentration gradients, pressure and temperature. The apparatus excludes additional backpressure regulators or pumps on individual channels. Also provided is a method of screening a sample, using supercritical chromatography, using the above apparatus, where multiple samples can be screened simultaneously with parallel processing.

The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for its preparation and separations devices containing the chromatographic material; separations devices, chromatographic columns and kits comprising the same; and methods for the preparation thereof. The chromatographic materials of the invention are superficially porous chromatographic particulate materials comprising sized less than 2 microns.

The invention discloses a five-zone series supercritical carbon dioxide fluid simulated moving bed chromatography device and an operation method of the five-zone series supercritical carbon dioxide fluid simulated moving bed chromatography device. A supercritical fluid chromatography device and a liquid phase simulated moving bed chromatography device are integrated into the five-zone series supercritical fluid simulated moving bed chromatography device, especially the five-zone series supercritical fluid simulated moving bed chromatography device with supercritical carbon dioxide fluid as a flowing phase. Target component can be separated and extracted from multiple components in a continuous operation mode, a pressure gradient operation mode and a modifier gradient operation mode, the components are flexibly connected in series in a 4+3 mode or a 3+3 mode, one component in any position can be conveniently separated from the components, efficient, low-consumption, clean and large-scale chromatography separation of multiple component raw materials can be achieved, and the five-zone series supercritical carbon dioxide fluid simulated moving bed chromatography device is especially suitable for industrial preparation separation and can bring evident economic and social benefits.

An apparatus, system, and process of converting a standard, high performance liquid chromatography (HPLC) flow path to a flow path suitable for supercritical fluid chromatography (SFC) are described. This reversible technique is applied to a variety of flow configurations including binary, high pressure solvent mixing systems and quaternary, low pressure solvent mixing systems than can be conventionally operated or automated. The technique is generally applied to the fields of supercritical fluid chromatography and high pressure liquid chromatography, but users skilled in the art will find utility for any flow system where pressurization components must be periodically applied to and removed from both ends of a flow stream in an automated manner.

A drive mechanism for back pressure regulator used in liquid chromatography, supercritical fluid chromatography, or supercritical fluid extraction allows very fine automated control over a very wide range of pressures by combining a linear actuator compressing a spring, pushing a pin. The nozzle assembly of the regulator comprises a flow through chamber containing a diaphragm and a seat, in which the pin pushes the diaphragm against the seat, together with an upstream pressure sensor and electronic feedback control to the motor of the actuator. The BPR of the embodiments exhibits high pressure stability and extremely low pressure noise, even at moderate to high pressures. The exemplary BPR can be use at either constant pressure or to generate pressure programs where the pressure is varied versus time. Further, the nozzle assembly has a field-replaceable head, requiring no mechanical adjustment on replacement.

The invention discloses a sesquiterpenoids compound which is of a novel structure and found in tobacco. The compound is named nicotianasesterpene H, the molecular formula of the compound is C<15>H<20>O<3>, and the structural formula can be found in the specification. The invention further discloses the application of the compound. Activity tests indicate that the compound has a good inhibiting effect on rotavirus and can serve as a lead anti-rotavirus compound for being used in research and development of anti-rotavirus medicinal preparations.

The invention discloses a sesquiterpene compound which is prepared through supercritical fluid chromatography and is novel in structure. The compound is named as nicotianasesterpene-F, wherein the molecular formula of nicotianasesterpene-F is C16H20O2, and the nicotianasesterpene-F has the following structure (please see the structure in the specification). The invention further discloses application of the compound. Activity tests show that nicotianasesterpene-F has a very good restraining effect on tobacco mosaic viruses and can be used for research and development on tobacco mosaic virus prevention pharmaceutic preparations as the lead compound for preventing tobacco mosaic viruses.

The invention discloses a method for preparing surface-closed medicine-carrying porous polymer microsphere based on a supercritical fluid technology. The method comprises the following steps of: dispersing a dichloromethane solution of a polymer, poloxamer serving as a pore-foaming agent and a medicinal mixture in a polyvinyl alcohol aqueous solution to form emulsion, performing water bath and drying to obtain a medicine-carrying communication porous polymer microsphere; putting the medicine-carrying communication porous polymer microsphere into a high-pressure autoclave; and introducing supercritical carbon dioxide to contact the medicine-carrying communication porous polymer microsphere fully, closing communication holes on the surface of polymer microsphere under the plasticizing action of the supercritical carbon dioxide on a polymer, and releasing pressure to form the surface-closed medicine-carrying porous polymer microsphere. The method has a simple operating process, is mild in operating conditions and stable in process; and by the method, solvent residues can be removed in the process for preparing the medicine-carrying communication porous polymer microsphere, the burst release of medicines is avoided, and the method can be applied to sustained and controlled release administration systems.

The invention relates to multifunctional heating pressurized extraction-trapping-chromatographic separation on-line combined equipment. The multifunctional heating pressurized extraction-trapping-chromatographic separation on-line combined equipment comprises four chromatographic pumps, three three-way mixers, two six-way switching valves, a heating pressurized extraction unit, a trapping column, a chromatographic column, a detector and the like. Through combined use of an on-line trapping-desorption-switching device and an extraction-chromatographic separation device, switching of the two high-pressure six-way switching valves and pressure adjustment of a back pressure regulator are controlled, the two extraction modes, including supercritical fluid extraction and accelerated solvent extraction, and the two chromatographic techniques, including supercritical fluid chromatography and high performance liquid chromatography, can be freely combined for use, functional diversity of the equipment is improved, and the multifunctional heating pressurized extraction-trapping-chromatographic separation on-line combined equipment can be effectively used for on-line extraction, trapping, desorption, separation and detection of trace objects in such complicated solid or semi-solid samples as environments, food, medicines and biology.

An apparatus, process, and system are disclosed that effectively provide separation of a combined gas/liquid flow stream into its separated gas and liquid factions. The invention is primarily directed to the fields of preparative supercritical fluid chromatography [SFC] and supercritical fluid extraction [SFE], but will have other utilization and applicability where phases of dramatically different density, viscosity and volumetric flow require separation.

The invention discloses a method for preparing core-shell type composite microspheres by a supercritical fluid technology. The method comprises the following steps of: dissolving a polymer into an organic solvent which cannot be used for dissolving core material particles; dispersing the core material particles into the organic solvent of the polymer to obtain a suspension; then putting the suspension in a suspension conveying device; pumping in supercritical pure fluid through the suspension conveying device to form a solvent resistant effect to ensure that a polymer precipitate is separated out and coated on the surfaces of the core material particles so as to form core-shell type composite microspheres. The invention can improve the drug encapsulation rate and the slow releasing effect.