7 results about "Supercritical fluid chromatography" patented technology

The invention belongs to the technical field of valves, and relates to a high-pressure collecting valve suitable for being applied to a liquid chromatography system, application of the high-pressure collecting valve and a supercritical fluid chromatography system. According to the invention, high-pressure collection and automatic collection of samples in the operation process of the supercritical fluid chromatography system can be realized; the valve cavity unit adopts a scheme that equal-length branches are uniformly distributed in a star shape, so that the volume of a channel in the valve body is reduced and rectified, the dead volume of a pipeline can be greatly reduced, cross contamination among different components is reduced or even eliminated, and the sample collection rate and purity of each component are improved; the collecting valve adopts a structure completely different from a ball valve, and is provided with a pressure regulation feedback system and a sealing ring, so that the fault rate and the maintenance cost of the collecting valve can be reduced, and the service life is prolonged. The whole collecting valve adopts a modular design, so that the maintenance cost caused by hardware damage due to long-time high-pressure operation of the supercritical fluid chromatography system can be effectively reduced. And a pressure closed-loop control system is arranged, so that the collection rate and the purity of the sample can be further improved.

This invention discloses a method for separating and preparing 4-aminoindane using a supercritical fluid chromatography (SCLC) system, comprising the following steps: dissolving an aminoindane sample; separating the aminoindane sample on a chromatographic column using a SCLC system to prepare 4-aminoindane; the mobile phase of the SCLC system is a mixture of supercritical CO2 and lower alcohols; the stationary phase of the SCLC system includes one or more of the following: silica gel surface-bonded phenyl groups, silica gel surface-bonded urea groups, or silica gel surface-bonded imidazole groups. This invention is the first to discover the use of a SCLC system for the separation and preparation of 4-aminoindane, supplementing existing high-performance liquid chromatography (HPLC); CO2 is more environmentally friendly, reducing harm to human health and the environment, and significantly reducing solvent processing costs; it can reduce the amount of chemical reagents used, reducing costs and increasing efficiency; the separation time is short, the separation effect is good, and the product purity can reach over 99%.

The present application is directed to methods, systems, and compositions related to and obtained from the supercritical fluid chromatography -based purification of saponins. In some embodiments, the present application is directed to methods of producing a purified saponin composition, the methods comprising applying a Quillaja saponin source composition to a supercritical fluid chromatography (SFC) stationary phase of a SFC system. In some embodiments, the present application is directed to such methods and applying an elution condition to the SFC stationary phase to produce the purified saponin composition. Also provided herein are SFC systems for practicing the methods described herein, as well as compositions and medicaments produced by the methods described herein.

This invention belongs to the field of tobacco chemical composition technology, specifically relating to a method for the simultaneous analysis of multiple fat-soluble components in tobacco leaves. The method includes the following steps: drying and grinding the tobacco leaves to be tested, adding an extractant and an internal standard to obtain a sample solution; analyzing the sample using a supercritical fluid chromatography system, employing multi-wavelength detection, and using the internal standard method. This invention provides a method for the simultaneous analysis of multiple fat-soluble components in tobacco leaves, addressing the common characteristics of fat-soluble components in tobacco leaves such as large molecular weight, poor volatility, and easy oxidation and decomposition. By using supercritical fluid chromatography, it can achieve simultaneous quantitative analysis of 13 fat-soluble components in tobacco, using less solvent, lower cost, and higher detection efficiency. This provides an environmentally friendly, more efficient, and convenient high-throughput analytical method for tobacco leaf quality evaluation.