1804 results about "Flavones" patented technology

The invention discloses piggy creep feed containing composite flavone. The piggy creep feed is prepared from the following raw materials in parts by weight: 13 to 20 parts of expanded corn, 10 to 25 parts of corn, 6 to 13 parts of broken rice, 8 to 12 parts of flour, 8 to 15 parts of whey mist, 3 to 8 parts of soy protein concentrate, 5 to 10 parts of extruded soybean, 0.7 to 3 parts of soya-bean oil, 7 to 12 parts of 46.5-percent bean pulp, 2 to 5 parts of fish meal, 2 to 5 parts of plasma protein powder, 0.3 to 0.7 part of mountain flour, 0.5 to 1.0 part of calcium hydrophosphate, 0.05 to 0.5 part of lysine, 0.05 to 0.3 part of threonine, 0.1 to 0.3 part of sodium chloride, 0.1 to 0.8 part of compound flavone premix compounds, 0.15 to 0.3 part of zinc oxide, 0.03 to 0.06 part of calcium propionate, 0.02 to 0.04 part of ethoxyquin and 1.0 part of suckling pig premix compounds. The invention also discloses a preparation method. The intestinal tract health and the production performance of weaned pigs can be improved.

The invention discloses a calendula double-bacterium fermentation product as well as a preparation method and application thereof, and relates to the technical field of plant fermentation. The preparation method of the calendula double-bacterium fermentation product disclosed by the invention comprises the following steps: carrying out primary fermentation by using lactobacillus plantarum, then carrying out secondary fermentation by using saccharomyces cerevisiae, and carrying out sterilization and filtration treatment on the calendula fermentation liquid subjected to secondary fermentation, so as to obtain the calendula fermentation liquid. The calendula fermentation liquor is subjected to combined fermentation through lactobacillus plantarum and saccharomyces cerevisiae, and the content of polyphenol and flavone in the calendula double-bacterium fermentation product is remarkably increased. Meanwhile, the calendula double-bacterium fermentation product has the effects of preserving moisture, resisting oxidation, relieving and controlling oil, and has a wide application prospect in the field of cosmetics.

The invention provides application of an enhanced and optimized mesenchymal stem cell exosome in knee osteoarthritis and muscle cartilage injury, and belongs to the technical field of biological medicine. The treatment activity of the umbilical cord mesenchymal stem cell exosome is remarkably improved by combining a composite pretreatment technology with a 3D culture system. Through the synergistic effect of the total flavonoids of rhizoma drynariae and tanshinone IIA, the pathological process of knee osteoarthritis can be regulated and controlled in multiple dimensions, including inflammatory reaction inhibition, cartilage repair promotion and joint function improvement, and the functional limitation of single-component pretreatment is broken through. The invention provides a safe and efficient novel treatment technical strategy for relieving knee osteoarthritis and muscle cartilage injury, and has a wide clinical application prospect.

The invention relates to the technical field of food, and particularly discloses a compound prebiotics embedded probiotic microcapsule and a preparation method and application thereof.The green tea leaves serve as main raw materials, water extraction is conducted on the green tea leaves, meanwhile, ultrasonic-assisted treatment is adopted, active ingredients in the green tea leaves can be more effectively released, the extraction time is shortened, and the content of probiotics in the green tea leaves is increased; the extraction rate of active ingredients in the green tea leaves is improved; then, lactobacillus acidophilus is adopted to ferment the extract liquid, so that the green tea fermentation liquid contains more antioxidant active ingredients, combined polyphenol in the extract liquid can release micromolecular polyphenol in the fermentation process, the content of substances such as flavone and polyphenol in the green tea fermentation liquid is increased, and the antioxidant activity is further improved; and then the modified dietary fiber is used as a main wall material of the microcapsule to embed the green tea fermentation liquor and the probiotics, so that the stability of active substances and the probiotics in the green tea fermentation liquor is improved, and the green tea fermentation liquor can play a role in protection and slow release in the simulated digestion process.

The invention discloses a plant-source nutritional supplement based on glycoside compounds and a preparation method thereof, and relates to the technical field of preparation of plant-source nutritional supplements.The preparation method comprises the steps that raw materials are prepared; the preparation method comprises the following steps: respectively carrying out ultrasonic-assisted extraction on flavonoid glycoside, saponin and geniposide raw materials to obtain an extracting solution; carrying out centrifugal separation on the extracting solution, taking supernate, and then purifying by adopting a macroporous resin adsorption method to obtain a purified glycoside compound solution; mixing the purified glycoside compound solution with lycium barbarum polysaccharide, Chinese yam starch, vitamin E and other auxiliaries in proportion, and uniformly stirring to form a mixed solution; carrying out spray drying on the mixed solution to obtain a powdery nutritional supplement; and screening the powdery nutritional supplement. The high-purity plant source glycoside compound and the functional auxiliary agent are scientifically compounded, and ultrasonic extraction, resin purification and precise process control are combined, so that the integrated improvement of activity synergy, quality stability and personalized scene adaptation of the nutritional supplement is realized.

The invention discloses a fructus psoraleae total flavone extract as well as a preparation method and application thereof in promoting hair growth. According to the preparation method of the fructus psoraleae total flavone extract, the specific eutectic solvent is adopted, the ultrasonic technology is combined for extraction, the content of total flavone in the fructus psoraleae extract can be remarkably increased, and the fructus psoraleae total flavone can be efficiently extracted; and the adopted extraction solvent has the advantages of greenness, environmental protection, efficient process, low cost, high safety and the like, and can solve the problems of the existing extraction process. The malaytea scurfpea fruit total flavone extract obtained by the method has the effect of promoting hair growth and can remarkably promote hair follicle cell proliferation and prolong the hair growth period, the prepared external gel product has the hair growth effect, the preparation method is simple, and external use is convenient. The malaytea scurfpea fruit total flavone extract provided by the invention can be used for developing more cosmetics and medical products for preventing alopecia and promoting hair growth, and a new scheme is provided for green extraction of natural active ingredients and treatment of alopecia.

The invention discloses an application of a CsHCT1 gene in increasing the content of citrus flavonoids, belongs to the technical field of agricultural biological genetic engineering, and provides an application of the CsHCT1 gene in increasing the content of citrus flavonoids, and the nucleotide sequence of the CsHCT1 gene is as shown in SEQ ID NO.1. The invention further discloses an application of the CsHCT1 gene in increasing the content of citrus flavonoids. The invention also discloses a method for improving the flavone compound of the citrus peel by using the CsHCT1 gene. The method comprises the following steps: cloning the citrus CsHCT1 gene; a CsHCT1 overexpression vector is constructed; transforming the overexpression vector to agrobacterium tumefaciens to obtain an agrobacterium tumefaciens liquid; and injecting the CsHCT1 gene into the citrus fruit to obtain the CsHCT1 gene overexpressed citrus fruit. A new choice is provided for increasing the flavonoid compounds in the citrus peel, the content of vicenin-2 and / or hesperidin in the citrus peel is effectively increased, and the application has great application value for cultivating new functional citrus varieties and accelerating the breeding process.

The invention discloses a method for extracting and purifying flavone in litsea coreana through an ultrasonic-assisted eutectic solvent, and belongs to the technical field of green processing of natural products. The invention provides a method for efficiently preparing litsea coreana flavone by integrating computer prediction, green solvent extraction and resin purification technologies. The method comprises the following steps: firstly, calculating and screening a deep eutectic solvent system with the highest affinity with the litsea coreana flavone on the basis of a COSMO-RS theoretical model, then coupling the optimized DES with an ultrasonic-assisted extraction (UAE) technology, and through the synergistic effect of the DES and the UAE technology, remarkably improving the extraction rate of the litsea coreana flavone and furthest keeping the biological activity of a target component; then, a macroporous resin dynamic adsorption-desorption purification process is linked, so that high-selectivity enrichment and impurity removal of the flavonoid compounds in the crude extract are realized, a high-purity product is obtained, and a complete process route from intelligent screening, efficient extraction to precise purification is finally formed.

A method for improving health conditions of mammals or livestock by feeding, to the mammals or livestock, a licorice extract including: (A) glycyrrhizic acid, a glycyrrhizic acid derivative, glycyrrhetinic acid, and / or a glycyrrhetinic acid derivative; (B) licorice saponins other than (A); and (C) licorice flavonoids; (B) including at least a licorice saponin H2, a licorice saponin G2, and macedonoside A, (C) including at least liquiritin apioside, isoliquiritin apioside, and isoliquiritin, where feeding is of 0.017 g / day / head or more of the licorice saponin H2, 0.002 g / day / head or more of the licorice saponin G2, and 0.002 g / day / head or more of the macedonoside A, 0.001 g / day / head or more of the liquiritin apioside, 0.001 g / day / head or more of the isoliquiritin apioside, and 0.001 g / day / head or more of the isoliquiritin.

The application discloses an Aspergillus niger strain and a culture method and application thereof, the Aspergillus niger strain is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 20241727. The fermented tea product obtained by fermenting tea leaves or tea leaf extracts by using the Aspergillus niger strain CCTCC NO: M 20241727 has prominent sweet and floral fragrance, and the astringency is obviously reduced; the contents of tea polyphenols, free amino acids and flavones are higher than those of a fermented tea product of an existing Aspergillus niger strain; the antioxidant activity is enhanced; the tea soup color is orange red and bright; and the color, fragrance and taste qualities are all superior to those of the fermented tea product of the existing Aspergillus niger strain.

The invention discloses an edible and medicinal composition for dispelling effects of alcohol and protecting liver as well as a preparation method and application thereof, and belongs to the technical field of functional foods. The composition consists of the following raw materials in parts by weight: 1-8 parts of ampelopsis grossedentata extract freeze-dried powder, 1-8 parts of astragalus membranaceus extract freeze-dried powder, 1-5 parts of kudzu vine root extract freeze-dried powder, 1-8 parts of hawthorn extract freeze-dried powder and 1-5 parts of cassia seed extract freeze-dried powder. The composition is designed according to a multi-component synergistic interaction principle, and the functions of protecting the liver and dispelling the effects of alcohol are enhanced through targeted interaction of active ingredients such as ampelopsis grossedentata flavone, puerarin and astragaloside; the compound preparation has the compound effects of protecting the liver, dispelling the effects of alcohol, resolving dampness, resisting oxidative stress, regulating lipid metabolism and the like, can remarkably relieve the problems of acute and chronic liver injury and metabolic disorder caused by alcohol intake, and meets the food-grade safety standard and the medicinal activity requirement; the important application prospect is realized in the aspect of preparing a preparation for relieving alcoholic liver injury and improving the liver detoxification function.

The invention discloses a flavonoid glycoside glycosyltransferase LbUGT71BX1 in laportea bulbifera as well as the coding gene and the use thereof. The amino acid sequence of the flavonoid glycoside glycosyltransferase LbUGT71BX1 is shown as SEQ ID NO: 2. The nucleotide sequence of the coding gene of the flavonoid glycoside glycosyltransferase LbUGT71BX1 is shown as SEQ ID NO: 1. According to the invention, on the basis of relevant results of the second-generation transcriptome and the third-generation full-length transcriptome sequencing of the laportea bulbifera, the last-step key enzyme LbUGT71BX1 for the synthesis of the flavonoid glycoside in the laportea bulbifera is screened and identified by using a reverse genetics method, filling the terminal blank of the biosynthesis pathway of the flavonoid glycoside in the laportea bulbifera.

The invention provides tartary buckwheat herbal baijiu and a preparation method thereof.The method aims at solving the problems that existing tartary buckwheat baijiu is lost in active ingredient, single in flavor and unstable in baijiu body, firstly, tartary buckwheat submicron powder, radix puerariae submicron powder and rhizoma polygonati submicron powder are put into Maotai-flavor base liquor together, and two-section type closed extraction is conducted at the normal temperature and then at the low temperature, adding a part of citric acid solution to adjust the pH at the initial stage of extraction; squeezing and filtering extract liquor, immediately adding bamboo leaf extract and phytic acid to carry out synergistic anti-oxidation treatment, and carrying out short-term aging; and finally, blending with the residual base wine. According to the preparation method disclosed by the invention, the retention rate of active ingredients such as tartary buckwheat flavone and the stability of the wine body are remarkably improved through staged extraction and dual antioxidant protection, the wine body is successfully endowed with coordinated baking fragrance, sauce fragrance and herbal flavor, and the taste is mellow and mild.

The invention discloses a supercritical entrainment extraction device for total flavonoids in folium cortex eucommiae, and relates to the technical field of pharmaceutical production. Comprising a fluid supply mechanism and an extraction mechanism, the fluid supply mechanism comprises a material mixing tank, a stirring pipe is rotatably arranged in the material mixing tank, a plurality of strip-shaped shells are fixedly arranged on the surface of the stirring pipe, and a plurality of nozzles are fixedly embedded in the surfaces of the strip-shaped shells. A dispersing disc and a grinding roller are arranged in the extraction tank, eucommia ulmoides leaves on the surface of the dispersing disc can be ground and smashed, and meanwhile, supercritical carbon dioxide mixed fluid is sprayed to the surfaces of the eucommia ulmoides leaves on the surface of the dispersing disc through a spray head on the surface of a rotating arm, so that the mixed fluid and the eucommia ulmoides leaves fully react; by utilizing the dissolving capacity of the supercritical carbon dioxide mixed fluid, a worker can conveniently extract the total flavonoids from the folium cortex eucommiae dissolved solution, so that the residual of solvent toxicity under the traditional solvent extraction condition can be effectively avoided, and meanwhile, the poison to a human body and the pollution to the environment in the extraction process are also prevented.

The invention discloses Kluffa yeast and application of the Kluffa yeast in preparation of red camellia fermentation liquor with anti-aging and repairing functions, the Kluffa yeast is used for repairing PR-LQX002, the Latin name of the Kluffa yeast is Lachancea kluyveri, and the preservation number of the Kluffa yeast is CCTCC NO: M 20241723. The invention also provides a red camellia fermentation broth, a composition containing the fermentation broth and application of the red camellia fermentation broth in preparation of products with anti-oxidation, anti-saccharification, anti-wrinkle or repair effects. The fermentation broth is prepared from the following raw materials: red camellia flowers, red camellia leaves and Kluffa yeast PR-LQX002. The Kluffa yeast can greatly promote fermentation of flowers and leaves of the red camellia, and the obtained red camellia fermentation liquor not only contains active substances such as flavone, polyphenol and free amino acid, but also contains lipid substances such as ceramide, and has multiple effects such as saccharification resistance, repair, wrinkle resistance and oxidation resistance.

The present invention discloses a dandelion flavonoid derivative, its preparation method, and application. The dandelion flavonoid derivative has the following molecular structure: The dandelion flavonoid derivative provided by the present invention has anti-inflammatory and lung nodule-removing effects, is simple to prepare, and has good biocompatibility, making it a promising new drug for eliminating lung nodules.

The invention belongs to the technical field of sleep-aiding oral liquids, and discloses a sleep-aiding nerve-calming oral liquid and a preparation process thereof, the oral liquid is composed of the following components: a plant fermentation broth, fructo-oligosaccharide, gamma-aminobutyric acid, casein hydrolysis peptide, a saussurea involucrata culture, and pyrroloquinoline quinone disodium salt; wherein the plant fermentation liquor is prepared from spina date seeds, poria cocos, lilies, Chinese dates, lotus seeds, lophatherum gracile, seville orange flowers, red roses with double petals, roselle, wheat, licorice roots, peach kernels, sweet-scented osmanthus and plant lactobacillus BXM2. The core component spina date seed saponin A is combined with gamma-aminobutyric acid to directly regulate the GABAergic nervous system, shorten the sleep latency and effectively help sleep and tranquilize the nerves; casein hydrolysis peptide promotes secretion of melatonin, and cooperates with the saussurea involucrata culture to resist oxidative stress, improve the sleep structure and restore circadian rhythm; small molecular active peptides and flavonoid glycosides are generated through fermentation, and anxiety mood, fatigue state and qi and blood circulation are improved; nano embedding and targeted delivery technologies are adopted in the whole process, the bioavailability is high, and the risk of drug dependence is avoided.

The invention relates to a flavonoid active fraction of an Abelmoschi corolla, a preparation method and an application thereof, and belongs to the field of traditional Chinese pharmaceutics. A high-purity Abelmoschi corolla flavonoid active fraction is prepared, which is found to have better therapeutic activity for kidney disease, and especially have a significant therapeutic effect for diabetic kidney disease and lupus nephritis, or contrast-induced kidney injury and eye diseases. Meanwhile, the Abelmoschi corolla flavonoid active fraction described in the invention also has good safety.

The invention discloses a method for extracting eucommia ulmoides with an intestinal repair function, and relates to the technical field of natural product extraction.The method comprises the steps that a fresh eucommia ulmoides raw material is subjected to low-temperature drying and smashing, a polysaccharide water extract and filter residues are obtained through water extraction, and then the flavone residual quantity in the polysaccharide water extract is synchronously detected; carrying out enzymolysis on the polysaccharide water extract through beta-glucanase to obtain a small molecular polysaccharide solution, and carrying out alcohol extraction on filter residues to obtain a flavone alcohol extract; the preparation method comprises the following steps: mixing the two components according to a ratio, inoculating compound probiotics for anaerobic fermentation, regulating according to the residual quantity of flavone, regulating an alcohol extraction process by using short-chain fatty acid generated by fermentation when the residual quantity is high, supplementing flavone extract in an enzymolysis system when the residual quantity is low, and finally removing impurities, purifying, adapting intestinal tracts and drying to obtain the product. According to the method, regulation and control operation is correspondingly executed by detecting the residual quantity of flavone in the polysaccharide water extract, so that self-supply of a regulation and control reagent and high efficiency of active ingredients are realized, the conversion rate of small molecular polysaccharide and flavone aglycone is increased, and the intestinal tract tolerance of the product is optimized.

The invention relates to the field of shampoo preparation, and particularly discloses a preparation method of anti-hair-loss, hair-growth and oil-control shampoo based on plant active ingredients, which comprises the following steps: step 1, soaking folium artemisiae argyi and pepper in warm water of 50-60 DEG C for 30 minutes, decocting for 30 minutes with medium fire, and filtering to obtain a first extracting solution; the preparation method comprises the following steps: soaking soapberry, cacumen biotae, duckweed, fructus cnidii, fructus kochiae, angelica sinensis, radix rehmanniae, radix paeoniae alba, rhizoma atractylodis macrocephalae, coptis chinensis, golden cypress, scutellaria baicalensis, radix sophorae flavescentis and ligusticum wallichii for 2 hours, and decocting at 95-100 DEG C for 4 hours; a functional closed loop of'oil control purification-hair follicle activation-inflammation blocking 'is formed through a three-element synergistic mechanism of sapindus, cacumen biotae and duckweed, sapindus saponin constructs microenvironment balance through targeted removal of hair follicle fat suppository, cacumen biotae flavone activates a hair papilla cell proliferation signal channel, duckweed polysaccharide forms an anti-inflammatory protection barrier, and the anti-inflammatory effect is achieved. The three components form a three-dimensional protective network on the molecular level, so that the pathological process of seborrheic alopecia is effectively blocked.

The invention relates to the technical field of medicine preparation, in particular to a preparation method of licoflavone. The preparation method comprises the following steps: providing a licorice raw material, crushing, sieving, and carrying out solid-state fermentation to obtain licorice powder after solid-state fermentation; the preparation method comprises the following steps: adding a first mixed organic solution into a reaction kettle, soaking, adding a second mixed organic solution into the first mixed aqueous solution again, soaking, adding a second mixed organic solution, extracting, collecting an organic phase, carrying out enzymolysis enzyme deactivation and impurity removal on a solid product, and adding the second mixed organic solution again for extracting; and combining the organic phases, dialyzing, purifying and concentrating to obtain the licoflavone.

The invention discloses preparation and application of an arundina graminifolia fermentation extract. The arundina graminifolia is fermented by adopting saccharomyces cerevisiae, so that the yield of total polyphenol (increased by 119.1%) and the yield of flavone (increased by 43.8%) in an arundina graminifolia extracting solution can be remarkably increased. The content of soluble solids in the obtained fermentation broth is 9.12 mg / mL, and the fermentation broth has acetylcholin esterase inhibitory activity (IC50 is 4.0 mg / mL) and oxidation resistance (the free radical scavenging rates IC50 of ABTS and DPPH are 0.116 mg / mL and 16.2 [mu] g / mL respectively); the fermentation liquor is high in sensory evaluation and palatability, and can be applied to nerve health maintenance in the field of homology of medicine and food as well as antioxidant functional beverages and health care products.

The invention belongs to the technical field of microorganisms, and relates to a plant lactobacillus NXU-F1 strain and application thereof. The classification name of the bacterial strain is lactobacillus plantarum, the bacterial strain is preserved in Guangdong Microbial Culture Collection Center on June 20, 2025, and the preservation number of the bacterial strain is GDMCC No.66563. The invention also discloses a preparation method of the bacterial strain. The plant lactobacillus is strong in growth capability, has good adaptive capacity to environment, and can obviously increase the content of antioxidant substances when being applied to plant matrix fermentation. The lycium barbarum and grape compound fermented beverage is prepared by fermenting the lycium barbarum and grape compound fermented beverage, the content of polyphenol, flavone, polysaccharide and procyanidine in the beverage is obviously increased, and the increase of resveratrol and ferulic acid is particularly obvious; meanwhile, ester compounds are increased and aldehyde substances are reduced, so that the beverage is endowed with strong longan fruity flavor, and the flavor of harmonious sour and sweet taste and lingering aftertaste is formed.

The invention discloses a skin anti-wrinkle whitening composition containing ginkgetin and a preparation method of the skin anti-wrinkle whitening composition. The composition takes ginkgo biflavone as a main active component, has the characteristics of safety and effectiveness, can remarkably improve skin elasticity and reduce wrinkles, and has the effect of whitening skin.

The invention discloses targeted prebiotic plant extraction dietary powder for relieving hyperuricemia and a preparation technology of the targeted prebiotic plant extraction dietary powder, and belongs to the technical field of dietary powder.In a formula of the targeted prebiotic plant extraction dietary powder for relieving hyperuricemia, as a patented bacterial strain, the bacillus coagulans MKSWC01 can directly act on the intestinal tract environment, uric acid precursor substances generated by purine metabolism are degraded in a targeted mode, and generation of uric acid is reduced from the source; the chicory root superfine powder and the mulberry leaf DNJ extract respectively activate kidney excretion protein, inhibit the activity of glycosidase, accelerate discharge of uric acid through urine and block the path of conversion from fructose metabolism to uric acid; the celery seed flavone and the smilax glabra polysaccharide further play an anti-inflammatory role, the deposition risk of urate crystals at joints is reduced, and potential inflammatory response caused by hyperuricemia is relieved. Meanwhile, the resistant dextrin and the xylooligosaccharide serve as prebiotics, nutrients are provided for probiotics to support planting of the probiotics, the overall metabolic environment can be improved by adjusting intestinal flora balance, the pathological link of hyperuricemia is comprehensively covered, and the relieving effect is improved.

The invention relates to the technical field of nutritional health-care food, and particularly discloses functional oral liquid capable of tonifying qi and blood and enhancing immunity based on ganoderma lucidum active ingredients and a preparation method. The composition comprises a ganoderma lucidum fermentation stock solution, ginseng, lucid ganoderma, astragalus membranaceus, angelica sinensis, fructus lycii, fructus hippophae, rhizoma polygonati, oyster nano peptide, raspberry and a chitosan oligosaccharide-vitamin E stabilizer. The preparation method comprises the following steps: crushing medicinal materials, performing graded extraction, performing ultrasonic integration with a ganoderma lucidum fermentation stock solution, performing membrane separation and purification, performing gradient vacuum concentration, adding a stabilizer under the protection of nitrogen, stirring, and finally performing nitrogen filling and pasteurization. The composition disclosed by the invention can be used for tonifying qi and blood and regulating immunity, the bioavailability is improved by virtue of synergy of ganoderma lucidum polysaccharide, ganoderma lucidum triterpenes and ginsenoside and an oyster nano peptide-sea buckthorn flavone composite carrier, and efficient retention and synergistic interaction of active ingredients are realized; in addition, low-temperature gradient concentration and mild sterilization are adopted in the preparation method, so that the heating time is shortened, and the quality stability of the product is ensured.

The invention discloses a method for improving the quality of phyllanthus emblica fruit juice through an enzymolysis-assisted pulsed electric field, which comprises the following steps: firstly, carrying out preliminary enzymolysis treatment on phyllanthus emblica fruits through a low-concentration compound enzyme solution to form micropores in the surfaces of cell walls; then, the pore diameter of the preliminarily formed micropore area is further expanded under the action of a pulsed electric field, the cell structure is destroyed to form a pore channel, through combined regulation and control of enzymolysis and electric field parameters, efficient release of micromolecular nutritional ingredients such as vitamin C, polyphenol substances, total flavonoids and monosaccharide is achieved through the synergistic effect of the enzymolysis and the electric field parameters, and the effect of improving the nutrient content is achieved. Meanwhile, the excessive precipitation of macromolecular polysaccharide is limited, the taste of the fruit juice is improved, the clarity of the fruit juice and the stability of nutritional ingredients are ensured, the consistency of the taste of the product is maintained, in addition, the enzyme dosage is effectively reduced, the safety problem caused by enzyme residues is reduced, the enzymolysis time and the pulse intensity are also remarkably reduced, and the production cost is reduced; the method meets the requirement of green production and is suitable for industrial large-scale application.

The invention relates to the technical field of aquaculture, in particular to a leiocassis longirostris intestinal health functional additive based on spermaceti soxhlet metabolite. The Leiocassis longirostris intestinal health functional additive comprises the following functional components: chondroitin sulfate, N-acetyl-D-galactosamine, 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4', 4 ', 4'- The composition is prepared from the following raw materials: 4, 6-dihydroxyflavone, polydatin, ascorbic acid, glucoside, arabinogalactan, 3-O-beta-D-galactopyranosyl-L-arabinose, tyramine, 4A-hydroxytetrahydrobiopterin, hyaluronic acid and 3-mercapto-2-butanol. According to the technical scheme, the technical problem that an existing probiotic viable bacterium preparation is difficult to meet the intestinal health management requirement in intensive culture of the leiocassis longirostris can be solved. Compared with the prior art, the scheme is more efficient, stable and safe, healthy development of the Leiocassis longirostris breeding industry can be effectively promoted, and the method has ideal application and popularization prospects.

The invention discloses cloning and functional identification of a liverwort flavone synthetase gene, and belongs to the technical field of gene engineering. According to the invention, one flavone synthase I (flavones synthase I, FNS I) is obtained through screening, and is named as MpFNS I; a prokaryotic expression system is utilized, an MpFNS I recombinant protein is obtained through purification, and the result of in-vitro enzyme activity identification shows that an MpFNS I enzyme substrate is relatively wide in selectivity, has a flavone synthetase function and also has a part of flavonol synthetase function. The liverwort flavonoid has a significant difference on affinity and catalytic efficiency of different substrates, the in-vivo function of the liverwort flavonoid is verified by utilizing heterologous expression of arabidopsis thaliana, experimental evidence is provided for comprehensively analyzing a biosynthetic pathway of the liverwort flavonoid, and meanwhile, a research target is provided for deeply researching a mechanism for synthesizing and responding to UV-B radiation of the liverwort flavonoid.