105 results about "Lactobacillus brevis" patented technology

The invention discloses a carbonyl reductase, a mutant and an application of the carbonyl reductase in synthesis of a chiral alcohol compound. The carbonyl reductase mutant is obtained by performing single-site or multi-site mutation on the 96th site, the 145th site, the 202nd site or the 206th site of the amino acid sequence of the carbonyl reductase LbCR derived from lactobacillus brevis as shown in SEQ ID No.2. The activity and stability of the carbonyl reductase LbCR mutant are obviously improved, and the carbonyl reductase LbCR mutant has a wide substrate spectrum. Compared with a wild type, the mutant LbCRM4 has the advantages that the enzyme activity is improved by 86.3 times, the substrate conversion rate is greater than 99%, the e.e.p value of the product is always kept at 99.5% or above, the space time yield reaches 1288.9 g / L / d, and the half-life period at 40 DEG C is 101.4 h and is improved by 83.5 times. The LbCR mutant has an industrial application prospect.

The preservation number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) NO.33629, the preservation date of the bacterial strain is February 24, 2025, the preservation classification of the bacterial strain is Lactobacillus sakei, and the preservation unit of the bacterial strain is China General Microbiological Culture Collection Center. The strain provided by the invention can reduce the concentration of blood uric acid, relieve kidney inflammation caused by hyperuricemia, improve the protein level of a kidney uric acid excretion channel and reduce the expression level of uric acid resorption protein.

The invention discloses a composition for treating insomnia. Comprising probiotic compounds: lactobacillus plantarum, bifidobacterium lactis, lactobacillus crispatus, lactobacillus acidophilus, lactobacillus rhamnosus, bifidobacterium bifidum, lactobacillus reuteri, lactobacillus casei, lactobacillus bulgaricus, lactobacillus fermentum, lactobacillus gasseri, lactococcus lactis, bifidobacterium longum, lactobacillus salivarius, bifidobacterium breve, streptococcus thermophilus and lactobacillus paracasei; lactobacillus helveticus; and traditional Chinese medicine components and prebiotics. Probiotics are matched with traditional Chinese medicines with homology of medicine and food, and corresponding prebiotics are supplemented, so that the balance of intestinal flora can be adjusted and information transmission between intestines and cranial nerves can be promoted through the synergistic effect of the probiotics and the corresponding prebiotics, and then gastrointestinal inflammation, ulcer, helicobacter pylori infection, constipation and insomnia are treated or improved; and treating or improving spleen and stomach disharmony, liver qi stagnation, excessive heart fire, fire excess from yin deficiency, heart-kidney disharmony caused by emotion, pressure, diseases and the like, and insomnia caused by the symptoms.

The invention discloses an application of Lactobacillus brevis 146 (Lactobacillus brevis 146) in improving the bioavailability of GABA (gamma-aminobutyric acid) (gamma-aminobutyric acid). According to the present invention, the specific Lactobacillus brevis 146 is adopted, such that the component of GABA is enriched, the GABA is more diversified, and the GABA, the dead bacteria component of the postbiotics, and the other metabolic components such as the active components such as polysaccharide are provided, such that the bioavailability of the GABA is improved; therefore, the lactobacillus brevis 146 and the GABA can be combined for use, and the lactobacillus brevis 146 can be used for preparing the medicine for treating the nervous system and intestinal diseases, and can be used for preparing food and health care products.

The invention belongs to the technical field of aquaculture, and particularly relates to a pearl grouper fry rearing method. According to the method, firstly, after the roes are hatched, the fries are soaked with a bactericide, harmful bacteria on the surfaces of the roes and in a hatching pond can be effectively killed, and a bacteria solution of lactobacillus brevis serves as the bactericide, so that the use of antibiotics is reduced while the growth of pathogenic bacteria is inhibited; the culture pond is divided into 1m * 1m spaces by the partition plates, so that the phenomenon that the fries kill each other or snatch for food due to insufficient spaces is avoided, and uniform growth of the fries is facilitated; culture is carried out in stages according to nutritional requirements of different growth stages, and the Weissella microbial inoculum and the lactococcus lactis microbial inoculum are added to the feed in each stage, so that the intestinal microecological balance of the fry is adjusted, the growth and reproduction of harmful bacteria are inhibited, the intestinal digestion and absorption functions of the fry are enhanced, and the utilization rate of the feed is increased.

The invention provides lactobacillus brevis and application thereof, the lactobacillus brevis is lactobacillus brevis RJS1401, is preserved in the China Center for Type Culture Collection in Wuhan on October 22, 2024, and has the preservation number of CCTCC NO: M 20242296. The lactobacillus brevis is lactobacillus brevis RJS1401, is preserved in the China Center for Type Culture Collection in Wuhan on October 22, 2024, and has the preservation number of CCTCC NO: M 20242296. The strain can effectively degrade organophosphorus and nitrite, shows good stress resistance and gamma-aminobutyric acid (GABA) production capacity, and has a remarkable peculiar smell modification effect; the method can be applied to preparation of edible plant enzymes, pickled vegetables and other products.

The invention discloses a traditional Chinese medicine and probiotic composition and application thereof, and belongs to the technical field of medicines. The composition is prepared from a traditional Chinese medicine extract and lactobacillus brevis DMU22441 (the preservation number is CGMCC No.65301), wherein the traditional Chinese medicine extract is prepared from fructus gardeniae, rhizoma phragmitis and flos sophorae according to the mass ratio of 1: 1: 1. The traditional Chinese medicine extract is prepared through an ethanol extraction method, and the viable count of probiotics is 10 < 9 > CFU / day. According to the composition, through a three-in-one synergistic mechanism of inhibiting synthesis, promoting excretion and regulating flora, the serum uric acid level is remarkably reduced, renal function impairment is improved, and intestinal flora imbalance is regulated. The invention provides an efficient and safe multi-target solution for the treatment of hyperuricemia and complications thereof, and has significant clinical application value.

The invention relates to the technical field of thallus quantity detection, in particular to a primer pair and a kit for detecting the number of lactobacillus brevis thalli in a postbiotic preparation and application of the primer pair and the kit. The primer pair provided by the invention has the advantages of strong specificity and high amplification efficiency, and can be used for carrying out qPCR quantitative detection on the quantity of lactobacillus brevis in the metastatic preparation. As the traditional flow cytometry and the plate cell counting method are not suitable for detecting the number of thalli in the metagen preparation, the qPCR quantitative method provided by the invention can be used for rapidly and accurately carrying out quantitative analysis on the number of lactobacillus brevis thalli in the metagen in a relatively short time, and the result is compared with a plate counting result; compared with the prior art, the qPCR counting method has the advantages that the detection result difference does not exceed one order of magnitude, counting does not distinguish viable bacteria and dead bacteria, and the qPCR counting method has the advantages of high specificity and high speed and can make up the limitation that a plate counting method is suitable for viable bacteria counting, so that the qPCR counting method provided by the invention has more advantages in the application of metagen counting.

A method for treating silage to enhance the aerobic stability by increasing the fermentation and stabilization of silage is disclosed. The method comprises treating silage or feed with a composition comprising one or more of a Lactobacillus brevis (Levilactobacillus brevis) strain LB7148, deposited as Patent Deposit No. NRRL B-67991 and Lactobacillus buchneri (Lentilactobacillus buchneri) strain LN7149, deposited as Patent Deposit No. NRRL B-67992, including mixtures or mutant(s) thereof which retain their silage preservative activity and / or or the anti-Acetobacter spp. components produced by LB7148 and LN7149, respectively. The strains of Lactobacillus buchneri (Lentilactobacillus buchneri) and Lactobacillus brevis (Levilactobacillus brevis) disclosed herein have been purified, isolated, and when applied to pre-ensiled plant material found to inhibit the growth of Acetobacter spp. and to improve aerobic stability of silage.

The invention discloses an application of lactobacillus brevis CCFM1267 capable of increasing the content of phytosphingosine in fermented milk, and belongs to the technical field of microbial fermentation. The invention provides a bacterial strain composition. The bacterial strain composition comprises lactobacillus brevis CCFM1267, streptococcus thermophilus CCFM1094 and lactobacillus delbrueckii subsp. Bulgaricus 2038. The strain composition is used for fermenting dairy products, and the content of phytosphingosine in the dairy products can be remarkably increased. Therefore, the sake lactobacillus brevis CCFM1267 or the strain composition has a huge application prospect in preparation of fermented dairy products.

The invention discloses a strain of Lactobacillus brevis as well as a high-density culture method and application thereof, the strain is Lactobacillus brevis X66, is obtained by separating and screening from a traditional dairy product dried milk cake in Inner Mongolia, is preserved in Guangdong Microbial Culture Collection Center on August 4, 2022, and has a preservation number of GDMCC No: 62641. The strain provided by the invention is safe, can tolerate gastrointestinal tract environment, has excellent self-coacervation capability and oxidation resistance, and can inhibit escherichia coli, staphylococcus aureus and pseudomonas aeruginosa. The invention provides a high-density culture method of the X66 strain, the viable count of X66 in optimized fermentation liquor exceeds 2 billion CFU / mL and is 2-3 times that before optimization, the viable count of freeze-dried powder prepared from the X66 strain reaches up to 10 billion CFU / g, and preconditions are provided for application of the strain in the fields of fermented dairy products, functional foods, nutritional dietary supplements and the like.

The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO.30979, the preservation date is June 17, 2024, the preservation classification is named as Lactobacillus brevis, and the preservation unit is the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. The strain is harmless to animals and has the remarkable effects of relieving lung tissue inflammatory infiltration caused by bronchial asthma, reducing release of serum IgE and Th2 type cell factors, inhibiting activation of an NF-kappa B channel and improving allergic airway inflammation. The invention also discloses application of the strain and the thallus component thereof in preparation of drugs for preventing and treating allergic asthma.

The present disclosure relates to an anti-fatigue Lactobacillus composition. The anti-fatigue Lactobacillus composition, which includes at least one of Lactobacillus brevis GKEX, Lactobacillus plantarum GKK1 and Lactobacillus johnsonii GKJ2 as an active ingredient, administered to a healthy subject for a continuous period of time, can significantly improve fatigue-related biochemical indices and prolong aerobic exercise time to exhaustion, and thus can be used as an active ingredient for preparation of various compositions for anti-fatigue and / or improving athletic ability.

The invention provides lactobacillus breviscapus for dispelling effects of alcohol and protecting liver and application thereof, and relates to the technical field of biological fermentation, the lactobacillus breviscapus is lactobacillus breviscapus, the strain number of the lactobacillus breviscapus is KMHD10-222, the registration number of the lactobacillus breviscapus in the China General Microbiological Culture Collection Center is CGMCC No.32358, and the number of the lactobacillus breviscapus in the China General Microbiological Culture Collection Center is KMHD10-222. The degradation rate of the lactobacillus brevicornu KMHD10-222 to ethanol with the concentration of 3% reaches 36.05%, the degradation rate of the lactobacillus brevicornu KMHD10-222 to ethanol with the concentration of 7% reaches 19.74%, and the lactobacillus brevicornu KMHD10-222 can degrade ethanol; the lactobacillus brevis KMHD10-222 microbial inoculum has the effect of relieving the acute alcoholic liver injury. The technical problem that in the prior art, probiotics capable of degrading ethyl alcohol and having the effects of dispelling the effects of alcohol and protecting the liver are lacked is solved.

Provided is a glucuronidase activator containing, as an active ingredient, Levilactobacillus brevis (Levilactobacillusbrevis) strain I-3141 (accession number: CECT 7480).

The present application relates to the field of agricultural microorganism technology, and discloses a strain of starch-degrading Levilactobacillus brevis AM515, which has a preservation number of CGMCC No. 33115, and the strain can have a degradation or structural modification capacity on corn starch in an anaerobic silage fermentation environment with a pH of 3.5-4.2. Development and application of a strain of Levilactobacillus brevis for improving in-vitro digestibility of whole-plant corn silage starch, the provided Levilactobacillus brevis AM515 (CGMCC No. 33115) is a functional strain directly screened from a silage environment. It can not only produce acid quickly like traditional lactic acid bacteria and dominate silage fermentation, but also has the ability to survive stably in a harsh low-pH (3.5-4.2) and anaerobic silage environment, degrade the starch content in corn silage feed, and improve the in-vitro digestibility of starch. This overcomes the dual technical obstacles of the difficulty of ordinary starch-degrading bacteria to adapt to the silage environment and the insufficient starch capacity of traditional silage lactic acid bacteria.

The invention discloses a lactobacillus brevis strain D15 for salt-reduced Pixian thick broad-bean sauce and application of the lactobacillus brevis strain D15. The preservation registration number of the strain D15 is CGMCC (China General Microbiological Culture Collection Center) No: 27698. The botanical classification of the strain D15 is short-growth-promoting lactobacillus in the bacterial field, the phylum of Thyrium, the Bacillus, the Lactobacillus, the Lactobacillus family and the Lactobacillus genus, and the Latin literature name of the strain D15 is Levibacillus brevis D15. The application of the strain D15 is as follows: firstly, the strain D15 is applied to preparation of salt-reduced Pixian thick broad-bean sauce; 2, application in preparation of salt-reduced chilli sauce; and 3, application in preparation of the salt-reduced broad bean sauce. After inoculation optimization is carried out on chili embryos or broad bean embryos by using the strain D15 disclosed by the invention, a salt-reduced fermentation system which takes chili and broad beans as raw materials and is under low salinity can be kept stable, the pH value is 4.2-4.5, and rancidity of the prepared fermented food is avoided; the enzyme activity can be obviously promoted, the fermentation time is shortened, the preparation cost is reduced, the flavor quality of the Pixian thick broad-bean sauce, the chili sauce and the broad bean sauce is obviously improved, and the industrial application value of the Pixian thick broad-bean sauce is obviously improved.

The invention discloses a composition with effects of reducing weight and reducing blood fat and application of the composition, and belongs to the technical field of biology. The composition comprises 30%-80% of a probiotic compound and 20%-70% of a raw material containing glycerol glycolipid, the probiotic compound takes lactobacillus reuteri as an essential component, and further comprises any one or more of bifidobacterium, lactobacillus fermentum, lactobacillus fermentum, lactobacillus acidophilus, lactobacillus casei, lactobacillus rhamnosus, lactobacillus brevis and bacillus coagulans. According to the invention, the specific probiotic compound is screened and compounded with the raw materials rich in glyceroglycolipid, and the synergistic effect of the probiotic compound and the raw materials is utilized to improve the weight-reducing and blood fat-reducing effects. According to the composition developed by the invention, the problem of resource waste caused by the fact that raw materials such as nannochloropsis oculata oil rich in glyceroglycolipid cannot be effectively absorbed in the prior art is solved, the nutritional effect of the composition is improved, and the composition has a wide application prospect in the fields of food, health care products and medicines.

The invention belongs to the technical field of health care products, and particularly relates to a composition containing probiotics. Comprising the following components in parts by weight: 5-10 parts of mixed zymolyte, 2-3 parts of lactobacillus acidophilus, 1-2 parts of bifidobacterium lactis, 1-2 parts of lactobacillus bulgaricus, 1-2 parts of lactobacillus brevis, 1-3 parts of lactobacillus delbrueckii, 1-2 parts of mannitol, 1-2 parts of isomaltooligosacharide and 3-7 parts of mixed leavening. The composition prepared by the invention has a very good weight-losing effect, and especially has a remarkable effect on a large cardinal number crowd with the weight of more than 80kg.

The present invention belongs to the field of microbial technology, and discloses a strain of Lactobacillus brevis and its antibacterial optimization culture method and application. The strain is Lactobacillus brevis (Lactobacillus brevis) L1612, which has been deposited in Guangdong Provincial Microbial Culture Collection Center on August 4, 2022, with a deposit number of GDMCC 62642. The strain of the present invention has broad-spectrum, efficient antibacterial properties, has the potential to produce new bacteriocins, and is safe and non-resistant. It can be used in the preparation of intestinal antibacterial drugs and in functional foods containing lactic acid bacteria. The present invention also provides an antibacterial optimization culture method for the bacteria, which uses MRS culture medium as a basal culture medium, optimizes the composition ratio and culture conditions in the basal culture medium, and makes the diameter of the inhibition zone of the bacterial fermentation broth increase significantly, which is 25.21% higher than before optimization, thereby facilitating the bacteria to be widely used in the fields of antibacterial drugs and functional food production.

The invention provides a lactobacillus brevis strain HBUR51234 as well as a screening method and an application method of the lactobacillus brevis strain HBUR51234. The lactobacillus brevis strain HBUR51234 is specifically the lactobacillus brevis HBUR51234, and the preservation number of the lactobacillus brevis strain HBUR51234 is GDMCC (Graphics Database Microorganism Center) No.65258. The lactobacillus brevis strain HBUR51234 is a lactobacillus brevis strain HBUR51234. The lactobacillus brevis strain HBUR51234 is a lactobacillus brevis strain HBUR51234. The strain can improve the quality and nutritional ingredients of the alfalfa silage, effectively optimizes the storage conditions of the silage, and has the effects of increasing the number of lactic acid bacteria and inhibiting growth of mould, saccharomycetes and some other pathogenic bacteria. According to the method, the agricultural measure requirement that alfalfa is harvested in a field and is directly subjected to silage production can be met, the variety of alfalfa silage is increased, the problems of preparation of high-moisture alfalfa silage and seasonal forage gaps of herbivores in winter and spring are effectively solved, and meanwhile, the resource recycling channel of low-grade and inferior golden-silk jujubes is expanded.

This invention discloses a fermentation method for improving the flavor and stability of old dough, belonging to the field of food technology. The method includes: mixing activated *Candida cruzi*, *Lactobacillus brevis*, *Hansenula polymorpha*, *Yarrowia lipolytica*, and *Saccharomyces cerevisiae* in a specific ratio to obtain a compound bacterial solution; and fermenting coarse grains with this compound bacterial solution to obtain coarse grain steamed buns. This invention employs the synergistic fermentation of five specific bacterial strains. *Lactobacillus brevis* and *Candida cruzi* synergistically optimize the rheological properties of the dough, *Hansenula polymorpha* and *Saccharomyces cerevisiae* synergistically produce rich aroma compounds, and *Yarrowia lipolytica* secretes phytase and cellolytic enzymes to lower the glycemic index. The coarse grain steamed buns prepared by this invention contain 55-65 flavor compounds, and the glycemic index is estimated to be reduced to 48-56, achieving a stable and controllable fermentation process, a low-GI product, and a rich flavor.

This invention relates to a blackcurrant and orange juice mixed soft drink, belonging to the field of beverage technology. The mixed soft drink contains blackcurrant extract, orange juice, white hyacinth bean seed extract, mogrosides, steviol glycosides, etc. The blackcurrant extract is a product with a lower than 5kDa obtained by enzymatic hydrolysis of blackcurrants with hemicellulase and pectinase, followed by sequential enzymatic hydrolysis with nattokinase, fig protease, papain, and glucokinase. The white hyacinth bean seed extract is a product with a lower than 5kDa obtained by trypsin hydrolysis of white hyacinth bean seeds, followed by co-fermentation with Propionibacterium finigense, Lactobacillus brevis, and Bifidobacterium longum. The blackcurrant extract and white hyacinth bean seed extract have excellent anti-fatigue, antioxidant, and immune-enhancing effects, making the mixed soft drink both delicious and healthy.

The invention discloses a lactobacillus brevis MyhYC-01, which is preserved in the China General Microbiological Culture Collection Center (CGMCC) on January 16, 2025, and the preservation number is CGMCC No.33441. The lactobacillus brevis MyhYC-01 is a lactobacillus brevis strain. The strain is screened from healthy Guayi lake ecological breeding river crabs, has better oxidation resistance, pathogenic bacterium inhibition and oxygen resistance, and can be used for effectively fermenting feed, pre-decomposing nutritional ingredients in the feed and improving the utilization efficiency of the feed. Through breeding experiments, the inventor finds that the strain has a relatively good effect on improving liver and intestine health and immunity of the river crabs. The lactobacillus brevis Myh YC-01 disclosed by the invention has the potential of serving as aquatic probiotics, and is very wide in application prospect.

The invention discloses fermented pickles and a preparation process thereof, relates to the technical field of pickles, and aims to solve the problems of unstable flavor, poor industrial adaptation and high labor cost of traditional pickles. The process comprises the following three steps: S1, mixing glucose, soybean meal enzymatic hydrolysate and other raw materials, carrying out two-step enzymolysis on soybean meal through alkaline protease and flavourzyme to obtain the soybean meal enzymatic hydrolysate, and adjusting the pH value to obtain a flavor base solution; s2, inoculating a mixed bacterium solution containing lactobacillus plantarum, lactobacillus brevis and saccharomycetes, performing anaerobic fermentation, then adding spices, and performing aroma enhancement fermentation in a micro-aerobic environment to prepare a flavor fermentation base solution; s3, putting vegetables into the flavor fermentation base solution for soaking, adding table salt for seasoning, and performing pasteurization to obtain a finished product. The core of the process is that the stable base solution is prepared firstly, and then the vegetables are soaked, the prepared pickled vegetables are bright in color, crisp and tender in taste, coordinated in flavor and high in industrial adaptability, harmful substances can be controlled, nutrition and oxidation resistance are improved, and the defects of a traditional process are overcome.

The invention is applicable to the technical field of microorganisms, and provides lactobacillus brevis DF-19 with high yield of tyramine and application of the lactobacillus brevis DF-19, the lactobacillus brevis DF-19 is preserved in China General Microbiological Culture Collection Center on May 26, 2025, and the preservation number is CGMCC No.33896. The strain DF-19 screened and separated by the embodiment of the invention has efficient tyramine production capacity, is easy to culture, has strong acid and salt resistance, specific metabolism, stable heredity and strong biological safety, and provides a basis for efficient production of tyramine and application and development of related products.

The invention relates to the technical field of microorganisms, in particular to lactobacillus brevis X4-4 and application of the lactobacillus brevis X4-4 in fermentation. The preservation number of the lactobacillus brevis X4-4 provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No. 31284. The activity of pyruvate dehydrogenase, malic acid-lactase, N-acetyltransferase and acid protease of the strain is remarkably higher than that of existing common lactobacillus, malic acid and protein in food raw materials can be efficiently utilized, and beneficial products can be generated. The lactobacillus brevis X4-4 has the specific advantages that 1) the lactobacillus brevis X4-4 can survive in a large quantity in an environment with relatively low pH (Potential of Hydrogen); (2) malic acid is converted into lactic acid which participates in pyruvic acid metabolism and arginine synthesis, so that the irritative sour feeling of the raw materials is reduced; (3) protein can be hydrolyzed into small molecule peptide under an acidic condition, so that the utilization rate of the protein is increased; and 4) the method can be used for single fruit raw materials or high-protein raw materials, and can also be used for fruit and protein composite raw materials.

The invention discloses green additive-free low-purine dried bean curds and a preparation method thereof. The preparation method comprises the following steps: S1, pretreating raw materials: cleaning soybeans, and soaking the cleaned soybeans in water; s2, grinding the soaked soybeans into raw soybean milk, heating and boiling the raw soybean milk, and cooling the raw soybean milk for later use; s3, inoculating the compound leavening agent into the cooled soybean milk, uniformly mixing, and fermenting at constant temperature to obtain fermented soybean milk; s4, the fermented soybean milk is sequentially subjected to curdling, forming, after-ripening and drying, and a finished product of the dried bean curd is prepared. The compound leavening agent comprises lactobacillus plantarum, rice koji and lactobacillus brevis. According to the method, lactobacillus plantarum, rice koji and lactobacillus brevis compound flora are used for fermentation, on the premise that no chemical agent is added, efficient degradation of purine and improvement of the oxidation resistance of the dried bean curd are synchronously achieved, the purine content of the product can be reduced to 80 mg / 100 g or below and is reduced by 50% or above compared with traditional dried bean curd, and the product quality is improved. The prepared product has the advantages of low purine, high oxidation resistance and excellent flavor, and is suitable for people with gout and hyperuricemia to eat.

The invention belongs to the field of micro-ecological feed additives, and discloses a leavening agent for whole corn silage. The leavening agent is composed of plant lactobacillus plantarum, lactobacillus brevis, leuconostoc mesenteroides and pediococcus acidilactici, strains of the plant lactobacillus plantarum, the lactobacillus brevis, the leuconostoc mesenteroides and the pediococcus acidilactici are fermented respectively, and then strain fermentation solutions obtained after fermentation are mixed according to the viable count ratio of (1-2): (1-2): (1-2): (1-2) to obtain the composite micro-ecological leavening agent. When the composite micro-ecological fermentation agent is used for whole-plant corn silage fermentation, the pH value can be rapidly reduced, the dry matter content can be increased, and the acid detergent fiber level can be reduced.

Disclosed herein is a method of making an antibiotic-resistant bacteria-free fermented food product to treat gut microbiota dysbiosis. Also disclosed is a fermented food product comprising a combination of unique starter strains of bacteria of Leuconostoc sp., Lactobacillus sp., Pediococcus sp., and / or other genera of lactic acid bacteria, such as Leuconostoc K6-Leu13 (WL-1) Leuconostoc mesenteroides Pediococcus K6-Pedio (WL-2), Pediococcus inopinatus, Lacticaseibacillus paracasei, Leuconostoc citreum, Lactobacillus SH16 (WL-3), Lactobacillus brevis, Pediococcus acidilactici, Latilactobacillus sakei, etc., for successful fermented products, with or without microbial succession.