142 results about "Lactobacillus brevis" patented technology

The invention discloses a carbonyl reductase, a mutant and an application of the carbonyl reductase in synthesis of a chiral alcohol compound. The carbonyl reductase mutant is obtained by performing single-site or multi-site mutation on the 96th site, the 145th site, the 202nd site or the 206th site of the amino acid sequence of the carbonyl reductase LbCR derived from lactobacillus brevis as shown in SEQ ID No.2. The activity and stability of the carbonyl reductase LbCR mutant are obviously improved, and the carbonyl reductase LbCR mutant has a wide substrate spectrum. Compared with a wild type, the mutant LbCRM4 has the advantages that the enzyme activity is improved by 86.3 times, the substrate conversion rate is greater than 99%, the e.e.p value of the product is always kept at 99.5% or above, the space time yield reaches 1288.9 g / L / d, and the half-life period at 40 DEG C is 101.4 h and is improved by 83.5 times. The LbCR mutant has an industrial application prospect.

The invention discloses banana resistant starch as well as a preparation method and application thereof in preparation of health-care food, and relates to the technical field of biology. The preparation method comprises the following steps: peeling immature bananas, performing irradiation disinfection, adding sterile water, performing grinding and pulping, and performing sieving with a 60-mesh sieve so as to obtain slurry; inoculating lactobacillus brevis and kluffa yeast into the slurry, and performing fermentation treatment to obtain fermentation liquor; standing the fermentation liquor, removing the supernatant, cleaning the precipitate with sterile water, and drying to obtain the banana resistant starch. The banana resistant starch prepared by performing combined fermentation treatment on lactobacillus brevis and Kluffa yeast can remarkably enhance the blood glucose metabolism regulation capability under the influence of high fat, so that the banana resistant starch can be applied to preparation of health-care food beneficial to maintaining the blood glucose health level. The method has important practical application prospects and is particularly suitable for market application and popularization.

The invention discloses a rhizoma smilacis glabrae leavening and a preparation method and application thereof in hyperuricemia, and the scheme is as follows: inoculating a lactobacillus fermentation seed solution into a rhizoma smilacis glabrae traditional Chinese medicine stock solution, fermenting for 48-72 hours at 37 DEG C, and freeze-drying to obtain the rhizoma smilacis glabrae leavening; the lactobacillus brevis fermentation seed liquid is prepared by fermenting lactobacillus brevis YM 1301 and lactobacillus plantarum CICC24202, and the lactobacillus brevis YM 1301 and the lactobacillus plantarum CICC24202 are fermented; the lactobacillus fermentation seed liquid accounts for 6-10% of the volume of the rhizoma smilacis glabrae traditional Chinese medicine Results show that after the rhizoma smilacis glabrae is fermented, the contents of total flavone and total polyphenol are increased, and the polysaccharide content is reduced; the rhizoma smilacis glabrae leavening can significantly reduce serum uric acid, urea nitrogen and creatinine levels of HUA rats; the kidney injury caused by hyperuricemia is improved; the increase of inflammatory cytokines such as IL-1beta and TNF-alpha in serum is reduced, and the curative effect is superior to that of unfermented rhizoma smilacis glabrae; the mechanism of reducing uric acid may be by inhibiting the activity of xanthine oxidase in HUA rats and increasing the excretion of uric acid in excrement. The invention provides a solid theoretical reference basis for developing safe and effective uric acid-reducing traditional Chinese medicine large-scale health products.

The preservation number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) NO.33629, the preservation date of the bacterial strain is February 24, 2025, the preservation classification of the bacterial strain is Lactobacillus sakei, and the preservation unit of the bacterial strain is China General Microbiological Culture Collection Center. The strain provided by the invention can reduce the concentration of blood uric acid, relieve kidney inflammation caused by hyperuricemia, improve the protein level of a kidney uric acid excretion channel and reduce the expression level of uric acid resorption protein.

The invention discloses a composition for treating insomnia. Comprising probiotic compounds: lactobacillus plantarum, bifidobacterium lactis, lactobacillus crispatus, lactobacillus acidophilus, lactobacillus rhamnosus, bifidobacterium bifidum, lactobacillus reuteri, lactobacillus casei, lactobacillus bulgaricus, lactobacillus fermentum, lactobacillus gasseri, lactococcus lactis, bifidobacterium longum, lactobacillus salivarius, bifidobacterium breve, streptococcus thermophilus and lactobacillus paracasei; lactobacillus helveticus; and traditional Chinese medicine components and prebiotics. Probiotics are matched with traditional Chinese medicines with homology of medicine and food, and corresponding prebiotics are supplemented, so that the balance of intestinal flora can be adjusted and information transmission between intestines and cranial nerves can be promoted through the synergistic effect of the probiotics and the corresponding prebiotics, and then gastrointestinal inflammation, ulcer, helicobacter pylori infection, constipation and insomnia are treated or improved; and treating or improving spleen and stomach disharmony, liver qi stagnation, excessive heart fire, fire excess from yin deficiency, heart-kidney disharmony caused by emotion, pressure, diseases and the like, and insomnia caused by the symptoms.

The invention discloses an application of Lactobacillus brevis 146 (Lactobacillus brevis 146) in improving the bioavailability of GABA (gamma-aminobutyric acid) (gamma-aminobutyric acid). According to the present invention, the specific Lactobacillus brevis 146 is adopted, such that the component of GABA is enriched, the GABA is more diversified, and the GABA, the dead bacteria component of the postbiotics, and the other metabolic components such as the active components such as polysaccharide are provided, such that the bioavailability of the GABA is improved; therefore, the lactobacillus brevis 146 and the GABA can be combined for use, and the lactobacillus brevis 146 can be used for preparing the medicine for treating the nervous system and intestinal diseases, and can be used for preparing food and health care products.

The present invention relates to the field of microorganisms, and in particular to a strain of Lactobacillus brevis ZG-7 and its application. The Lactobacillus brevis ZG-7 has been preserved in the Guangdong Provincial Microbial Culture Collection Center on June 14, 2023, with a preservation number of GDMCC No.63562. The Lactobacillus brevis ZG-7 of the present invention has a strong inhibitory ability against Salmonella typhimurium, and has good acid and alkali resistance and bile salt resistance, and the inhibitory property has good thermal stability and ultraviolet stability. The Lactobacillus brevis ZG-7 of the present invention can be used to regulate the microecological balance in the intestine of animals, enhance the nonspecific immune function of the body to prevent diseases, and can also provide nutritional factors, promote the digestion and absorption of nutrients, promote animal growth and improve feed conversion rate.

The invention discloses lactobacillus brevis JS231 for high yield of gamma-aminobutyric acid and application of the lactobacillus brevis JS231, and belongs to the technical field of biology, the lactobacillus brevis JS2311 is preserved in Guangdong Microbial Culture Collection Center on August 30, 2024, the preservation address is building 59, No. 100 Courtyard, Xianlie Middle Road, Guangzhou, and the preservation number is GDMCC 65079. The invention also provides any one of the following applications of the lactobacillus brevis JS231: (1) application in production of gamma-aminobutyric acid; (2) application in preparation of a fermentation product with antioxidant activity; (3) application in inhibiting the activity of pathogenic bacteria; (4) application in degradation of nitrite; and (5) application in development of lactic acid bacteria products rich in gamma-aminobutyric acid. The lactobacillus brevis JS231 provided by the invention provides an original material for the development of lactic acid bacteria resources.

The present invention relates to an enzyme catalyst for preparing a dicarboxylic acid and a method for preparing a dicarboxylic acid using the same. The enzyme catalyst is prepared by a method comprising the following steps: (1) carrying out fermentation culture on enzyme-producing microorganisms in a culture medium to obtain a crude enzyme solution, and (2) immobilizing the crude enzyme solution on a molecular sieve to obtain the enzyme catalyst, wherein the enzyme producing microorganisms are selected from candida tropicalis, candida sake, pichia pastoris, saccharomyces cerevisiae, lactobacillus brevis, corynebacterium glutamicum, bacillus subtilis, bacillus cereus, escherichia coli and pseudomonas aeruginosa. The method comprises the following steps: in a PBS buffer solution, mixing triglyceride with the enzyme catalyst, and carrying out catalytic reaction under stirring at 20-50 DEG C; and then carrying out hydrolysis reaction at the temperature of 250-300 DEG C under the pressure of 30-100 bar. According to the method, the enzyme catalyst and the molecular sieve are immobilized, so that other oxidants or catalysts are not required to be added while the dicarboxylic acid with higher yield is obtained.

The invention discloses a low-salt rapid bamboo shoot fermentation method, which adopts an inoculated fermentation mode, uses lactobacillus plantarum and lactobacillus brevis as leavening agents, and uses fresh bamboo shoots as raw materials. The preparation method comprises the following steps: selecting fresh bamboo shoots with high tenderness, peeling off bamboo shoot shells, cutting off bases, cleaning, and cutting into bamboo shoot slices with uniform sizes; cooking in boiling water for 5 minutes, fishing out, and airing to remove moisture; preparing water with the salt concentration of 2% and the glucose concentration of 2%, boiling the water, and airing the water for later use to obtain a fermentation feed liquid; adding the air-dried fresh bamboo shoot slices and the prepared material liquid into a bag which is scalded by boiling water and cooled according to a solid-to-liquid ratio of 1: 4, and then adding a lactic acid bacteria leavening agent into the fermentation bag for sealed fermentation; and placing the fermentation bag in an environment with a temperature of 30 DEG C for fermentation for 5 days. The prepared fermented bamboo shoots are short in fermentation period, low in nitrite content, mellow and normal in sourness, rich in flavor and high in sensory quality and food safety, and can better meet industrial and consumer requirements.

The invention belongs to the technical field of aquaculture, and particularly relates to a pearl grouper fry rearing method. According to the method, firstly, after the roes are hatched, the fries are soaked with a bactericide, harmful bacteria on the surfaces of the roes and in a hatching pond can be effectively killed, and a bacteria solution of lactobacillus brevis serves as the bactericide, so that the use of antibiotics is reduced while the growth of pathogenic bacteria is inhibited; the culture pond is divided into 1m * 1m spaces by the partition plates, so that the phenomenon that the fries kill each other or snatch for food due to insufficient spaces is avoided, and uniform growth of the fries is facilitated; culture is carried out in stages according to nutritional requirements of different growth stages, and the Weissella microbial inoculum and the lactococcus lactis microbial inoculum are added to the feed in each stage, so that the intestinal microecological balance of the fry is adjusted, the growth and reproduction of harmful bacteria are inhibited, the intestinal digestion and absorption functions of the fry are enhanced, and the utilization rate of the feed is increased.

The invention discloses lactobacillus brevis and application thereof, and belongs to the technical field of food microorganisms. The invention provides a lactobacillus brevis CHOL1, which is separated from pickled Chinese cabbages, can be applied to food through safety evaluation, has relatively strong nitrite degradation capability, can react with myoglobin to generate nitrosylmyoglobin, and can be used for degrading nitrite in food. After being inoculated into the fermented meat product, the strain can reduce nitrite and nitrosamine residues in the fermented meat product and improve the color and luster of the meat product at the same time. As a leavening agent, the strain can improve the quality and safety of fermented meat products, and can be used as a natural effective substitute for partially replacing nitrite in the fermented meat products.

The invention provides lactobacillus brevis and application thereof, the lactobacillus brevis is lactobacillus brevis RJS1401, is preserved in the China Center for Type Culture Collection in Wuhan on October 22, 2024, and has the preservation number of CCTCC NO: M 20242296. The lactobacillus brevis is lactobacillus brevis RJS1401, is preserved in the China Center for Type Culture Collection in Wuhan on October 22, 2024, and has the preservation number of CCTCC NO: M 20242296. The strain can effectively degrade organophosphorus and nitrite, shows good stress resistance and gamma-aminobutyric acid (GABA) production capacity, and has a remarkable peculiar smell modification effect; the method can be applied to preparation of edible plant enzymes, pickled vegetables and other products.

The invention discloses a traditional Chinese medicine and probiotic composition and application thereof, and belongs to the technical field of medicines. The composition is prepared from a traditional Chinese medicine extract and lactobacillus brevis DMU22441 (the preservation number is CGMCC No.65301), wherein the traditional Chinese medicine extract is prepared from fructus gardeniae, rhizoma phragmitis and flos sophorae according to the mass ratio of 1: 1: 1. The traditional Chinese medicine extract is prepared through an ethanol extraction method, and the viable count of probiotics is 10 < 9 > CFU / day. According to the composition, through a three-in-one synergistic mechanism of inhibiting synthesis, promoting excretion and regulating flora, the serum uric acid level is remarkably reduced, renal function impairment is improved, and intestinal flora imbalance is regulated. The invention provides an efficient and safe multi-target solution for the treatment of hyperuricemia and complications thereof, and has significant clinical application value.

The invention relates to the technical field of thallus quantity detection, in particular to a primer pair and a kit for detecting the number of lactobacillus brevis thalli in a postbiotic preparation and application of the primer pair and the kit. The primer pair provided by the invention has the advantages of strong specificity and high amplification efficiency, and can be used for carrying out qPCR quantitative detection on the quantity of lactobacillus brevis in the metastatic preparation. As the traditional flow cytometry and the plate cell counting method are not suitable for detecting the number of thalli in the metagen preparation, the qPCR quantitative method provided by the invention can be used for rapidly and accurately carrying out quantitative analysis on the number of lactobacillus brevis thalli in the metagen in a relatively short time, and the result is compared with a plate counting result; compared with the prior art, the qPCR counting method has the advantages that the detection result difference does not exceed one order of magnitude, counting does not distinguish viable bacteria and dead bacteria, and the qPCR counting method has the advantages of high specificity and high speed and can make up the limitation that a plate counting method is suitable for viable bacteria counting, so that the qPCR counting method provided by the invention has more advantages in the application of metagen counting.

A method for treating silage to enhance the aerobic stability by increasing the fermentation and stabilization of silage is disclosed. The method comprises treating silage or feed with a composition comprising one or more of a Lactobacillus brevis (Levilactobacillus brevis) strain LB7148, deposited as Patent Deposit No. NRRL B-67991 and Lactobacillus buchneri (Lentilactobacillus buchneri) strain LN7149, deposited as Patent Deposit No. NRRL B-67992, including mixtures or mutant(s) thereof which retain their silage preservative activity and / or or the anti-Acetobacter spp. components produced by LB7148 and LN7149, respectively. The strains of Lactobacillus buchneri (Lentilactobacillus buchneri) and Lactobacillus brevis (Levilactobacillus brevis) disclosed herein have been purified, isolated, and when applied to pre-ensiled plant material found to inhibit the growth of Acetobacter spp. and to improve aerobic stability of silage.

The invention discloses an application of lactobacillus brevis CCFM1267 capable of increasing the content of phytosphingosine in fermented milk, and belongs to the technical field of microbial fermentation. The invention provides a bacterial strain composition. The bacterial strain composition comprises lactobacillus brevis CCFM1267, streptococcus thermophilus CCFM1094 and lactobacillus delbrueckii subsp. Bulgaricus 2038. The strain composition is used for fermenting dairy products, and the content of phytosphingosine in the dairy products can be remarkably increased. Therefore, the sake lactobacillus brevis CCFM1267 or the strain composition has a huge application prospect in preparation of fermented dairy products.

The invention provides application of manuka honey in skin antibiosis and anti-inflammation, and belongs to the technical field of cosmetics, the application comprises the following steps: (1) mixing rose petals, perilla roots, grape seeds and manuka honey, and adding the mixture into water to obtain an enzymolysis substrate; (2) sequentially adding pectinase, neutral protease and beta-glucanase into the enzymolysis substrate, and performing enzymolysis to obtain an enzymolysis product; (3) adding lactobacillus brevis, lactobacillus helveticus and bifidobacterium lactis into the enzymolysis product, and fermenting to obtain a fermentation product; (4) adding an ethanol aqueous solution into the fermentation product, stirring, filtering by using a filter membrane to obtain a supernatant, and concentrating the supernatant to obtain a mixed extract; and (5) preparing skin antibacterial and anti-inflammatory products from the mixed extract. The mixed extract disclosed by the invention is good in batch stability, relatively good in antibacterial and anti-inflammatory effects and low in irritation.

The invention discloses a strain of Lactobacillus brevis as well as a high-density culture method and application thereof, the strain is Lactobacillus brevis X66, is obtained by separating and screening from a traditional dairy product dried milk cake in Inner Mongolia, is preserved in Guangdong Microbial Culture Collection Center on August 4, 2022, and has a preservation number of GDMCC No: 62641. The strain provided by the invention is safe, can tolerate gastrointestinal tract environment, has excellent self-coacervation capability and oxidation resistance, and can inhibit escherichia coli, staphylococcus aureus and pseudomonas aeruginosa. The invention provides a high-density culture method of the X66 strain, the viable count of X66 in optimized fermentation liquor exceeds 2 billion CFU / mL and is 2-3 times that before optimization, the viable count of freeze-dried powder prepared from the X66 strain reaches up to 10 billion CFU / g, and preconditions are provided for application of the strain in the fields of fermented dairy products, functional foods, nutritional dietary supplements and the like.

The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO.30979, the preservation date is June 17, 2024, the preservation classification is named as Lactobacillus brevis, and the preservation unit is the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms. The strain is harmless to animals and has the remarkable effects of relieving lung tissue inflammatory infiltration caused by bronchial asthma, reducing release of serum IgE and Th2 type cell factors, inhibiting activation of an NF-kappa B channel and improving allergic airway inflammation. The invention also discloses application of the strain and the thallus component thereof in preparation of drugs for preventing and treating allergic asthma.

The present disclosure relates to an anti-fatigue Lactobacillus composition. The anti-fatigue Lactobacillus composition, which includes at least one of Lactobacillus brevis GKEX, Lactobacillus plantarum GKK1 and Lactobacillus johnsonii GKJ2 as an active ingredient, administered to a healthy subject for a continuous period of time, can significantly improve fatigue-related biochemical indices and prolong aerobic exercise time to exhaustion, and thus can be used as an active ingredient for preparation of various compositions for anti-fatigue and / or improving athletic ability.

The invention provides lactobacillus breviscapus for dispelling effects of alcohol and protecting liver and application thereof, and relates to the technical field of biological fermentation, the lactobacillus breviscapus is lactobacillus breviscapus, the strain number of the lactobacillus breviscapus is KMHD10-222, the registration number of the lactobacillus breviscapus in the China General Microbiological Culture Collection Center is CGMCC No.32358, and the number of the lactobacillus breviscapus in the China General Microbiological Culture Collection Center is KMHD10-222. The degradation rate of the lactobacillus brevicornu KMHD10-222 to ethanol with the concentration of 3% reaches 36.05%, the degradation rate of the lactobacillus brevicornu KMHD10-222 to ethanol with the concentration of 7% reaches 19.74%, and the lactobacillus brevicornu KMHD10-222 can degrade ethanol; the lactobacillus brevis KMHD10-222 microbial inoculum has the effect of relieving the acute alcoholic liver injury. The technical problem that in the prior art, probiotics capable of degrading ethyl alcohol and having the effects of dispelling the effects of alcohol and protecting the liver are lacked is solved.

Provided is a glucuronidase activator containing, as an active ingredient, Levilactobacillus brevis (Levilactobacillusbrevis) strain I-3141 (accession number: CECT 7480).

The invention relates to lactobacillus brevis, the name of the lactobacillus brevis is lactobacillus brevis HSDF-1bn-2023, the preservation number is CGMCC No.30582, the lactobacillus brevis is preserved in China General Microbiological Culture Collection Center (CGMCC) on May 10, 2024, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Beichen West Road, Chaoyang District, Beijing, China. According to the present invention, the Lactobacillus brevis separated from the naturally fermented Chinese sauerkraut can be used for inhibiting Pichiakudriavzevii HSDF-pks-2023 (pks) causing the putrefaction of the Chinese sauerkraut, and the Lactobacillus brevis can be used for inhibiting the Pichiakudriavzevii HSDF-pks-2023 (pks).

The present application relates to the field of agricultural microorganism technology, and discloses a strain of starch-degrading Levilactobacillus brevis AM515, which has a preservation number of CGMCC No. 33115, and the strain can have a degradation or structural modification capacity on corn starch in an anaerobic silage fermentation environment with a pH of 3.5-4.2. Development and application of a strain of Levilactobacillus brevis for improving in-vitro digestibility of whole-plant corn silage starch, the provided Levilactobacillus brevis AM515 (CGMCC No. 33115) is a functional strain directly screened from a silage environment. It can not only produce acid quickly like traditional lactic acid bacteria and dominate silage fermentation, but also has the ability to survive stably in a harsh low-pH (3.5-4.2) and anaerobic silage environment, degrade the starch content in corn silage feed, and improve the in-vitro digestibility of starch. This overcomes the dual technical obstacles of the difficulty of ordinary starch-degrading bacteria to adapt to the silage environment and the insufficient starch capacity of traditional silage lactic acid bacteria.

The invention discloses a lactobacillus brevis strain D15 for salt-reduced Pixian thick broad-bean sauce and application of the lactobacillus brevis strain D15. The preservation registration number of the strain D15 is CGMCC (China General Microbiological Culture Collection Center) No: 27698. The botanical classification of the strain D15 is short-growth-promoting lactobacillus in the bacterial field, the phylum of Thyrium, the Bacillus, the Lactobacillus, the Lactobacillus family and the Lactobacillus genus, and the Latin literature name of the strain D15 is Levibacillus brevis D15. The application of the strain D15 is as follows: firstly, the strain D15 is applied to preparation of salt-reduced Pixian thick broad-bean sauce; 2, application in preparation of salt-reduced chilli sauce; and 3, application in preparation of the salt-reduced broad bean sauce. After inoculation optimization is carried out on chili embryos or broad bean embryos by using the strain D15 disclosed by the invention, a salt-reduced fermentation system which takes chili and broad beans as raw materials and is under low salinity can be kept stable, the pH value is 4.2-4.5, and rancidity of the prepared fermented food is avoided; the enzyme activity can be obviously promoted, the fermentation time is shortened, the preparation cost is reduced, the flavor quality of the Pixian thick broad-bean sauce, the chili sauce and the broad bean sauce is obviously improved, and the industrial application value of the Pixian thick broad-bean sauce is obviously improved.

The invention discloses a composition with effects of reducing weight and reducing blood fat and application of the composition, and belongs to the technical field of biology. The composition comprises 30%-80% of a probiotic compound and 20%-70% of a raw material containing glycerol glycolipid, the probiotic compound takes lactobacillus reuteri as an essential component, and further comprises any one or more of bifidobacterium, lactobacillus fermentum, lactobacillus fermentum, lactobacillus acidophilus, lactobacillus casei, lactobacillus rhamnosus, lactobacillus brevis and bacillus coagulans. According to the invention, the specific probiotic compound is screened and compounded with the raw materials rich in glyceroglycolipid, and the synergistic effect of the probiotic compound and the raw materials is utilized to improve the weight-reducing and blood fat-reducing effects. According to the composition developed by the invention, the problem of resource waste caused by the fact that raw materials such as nannochloropsis oculata oil rich in glyceroglycolipid cannot be effectively absorbed in the prior art is solved, the nutritional effect of the composition is improved, and the composition has a wide application prospect in the fields of food, health care products and medicines.

The invention belongs to the technical field of food, and relates to a method for fermenting air-dried sausages by completely replacing nitrite. According to the invention, lactobacillus fermentum CGMCC 1.1880, lactobacillus brevis CHOL1 and carrot powder are mixed to completely replace nitrite for food fermentation. The method can completely replace nitrite to promote the color development of the fermented meat product, so that the chromaticity can reach the effect similar to that of adding 140 mg / kg of sodium nitrite in meat, the residual quantity of nitrite in the fermented meat product is reduced, and the possibility that secondary amine in the nitrite and the meat reacts to generate a cancerogenic substance N-nitrosamine is further reduced; the residual quantity of nitrite in the air-dried sausage does not exceed 2mg / kg of meat, and the content of nitrosamine does not exceed 2.5 mu g / kg of meat. The method is of great significance in improving the safety of fermented food.

The invention belongs to the technical field of health care products, and particularly relates to a composition containing probiotics. Comprising the following components in parts by weight: 5-10 parts of mixed zymolyte, 2-3 parts of lactobacillus acidophilus, 1-2 parts of bifidobacterium lactis, 1-2 parts of lactobacillus bulgaricus, 1-2 parts of lactobacillus brevis, 1-3 parts of lactobacillus delbrueckii, 1-2 parts of mannitol, 1-2 parts of isomaltooligosacharide and 3-7 parts of mixed leavening. The composition prepared by the invention has a very good weight-losing effect, and especially has a remarkable effect on a large cardinal number crowd with the weight of more than 80kg.

The present invention belongs to the field of microbial technology, and discloses a strain of Lactobacillus brevis and its antibacterial optimization culture method and application. The strain is Lactobacillus brevis (Lactobacillus brevis) L1612, which has been deposited in Guangdong Provincial Microbial Culture Collection Center on August 4, 2022, with a deposit number of GDMCC 62642. The strain of the present invention has broad-spectrum, efficient antibacterial properties, has the potential to produce new bacteriocins, and is safe and non-resistant. It can be used in the preparation of intestinal antibacterial drugs and in functional foods containing lactic acid bacteria. The present invention also provides an antibacterial optimization culture method for the bacteria, which uses MRS culture medium as a basal culture medium, optimizes the composition ratio and culture conditions in the basal culture medium, and makes the diameter of the inhibition zone of the bacterial fermentation broth increase significantly, which is 25.21% higher than before optimization, thereby facilitating the bacteria to be widely used in the fields of antibacterial drugs and functional food production.