31 results about "Lactobacillus jensenii" patented technology

Use of an association of lactobacilli for preparation of a pharmaceutical composition for treatment of vaginosis and vaginitis. Said bacteria association comprises the Lactobacillus brevis and Lactobacillus salivarius subs. salicinius species, possibly in combination with one or more species selected from Lactobacillus salivarius subs. salivarius, Lactobacillus jensenii, Lactobacillus catenaforme, Lactobacillus minutus and Lactobacillus gasseri.A pharmaceutical composition comprising said association of lactobacilli adapted for treatment of vaginosis and vaginitis.A method of treating vaginosis and vaginitis based on administration of said pharmaceutical composition.

The invention discloses lactobacillus jensenii and application thereof. The lactobacillus jensenii is particularly lactobacillus jensenii IMJ-1, and the file No. of the lactobacillus jensenii IMJ-1 in the General Microbiological Culture Collection Center of China Microbial Culture Collection Management Committee is CGMCC No. 6558. The lactobacillus jensenii IMJ-1 CGMCC No.6558 has high H2O2 generation capability, can effectively suppress breeding of external pathogenic bacteria and other conditional pathogenic bacteria, and has a high binding function to epithelial cells of vagina, and accordingly, breeding of bacteria strains on the vaginal epithelium is guranteed. Besides, the lactobacillus jensenii IMJ-1 CGMCC No. 6558 has a certain application prospect in preventing and curing bacterial vaginosis.

The invention discloses a multi-joint bacterium lacticum composition. The multi-joint bacterium lacticum composition is an advantage bacterial strain selected by a separating screen in the vagina of ahealthy female in China and at least comprises three active components of Lactobacillus johnsonii (Lactobacillus johnsonii), Lactobacillusgasseri (Lactobacillusgasseri), Lactobacillus rhamnosus ( Lactobacillus rhamnosus ), Lactobacillus crispatus ( Lactobacillus crispatus ) and Lactobacillus jensenii (Lactobacillus jensenii ).

The invention provides a micro-ecological vaginal bacterial agent. The agent is prepared from a fermented liquid prepared from Lactobacillus crispatus and Lactobacillus jensenii through compatible fermentation. The invention further provides a preparation method of the micro-ecological vaginal bacterial agent. According to the method, Lactobacillus crispatus and Lactobacillus jensenii are mixed to be compatible, an enrichment culture medium is inoculated with the mixed bacterial liquid for enrichment culture, and an enrichment culture liquid is obtained; an enlargement culture medium is inoculated with the enrichment culture liquid for enlargement culture, and an enlargement culture liquid is obtained; a fermentation culture medium is inoculated with the enlargement culture liquid for fermentation culture, and the obtained fermented liquid is the micro-ecological vaginal bacterial agent. The micro-ecological vaginal bacterial agent is applied to preparation of drugs for preventing and treating gynecological diseases such as recurrent vaginitis or discharge abnormality. With the micro-ecological vaginal bacterial agent, the cure rate ranges from 88% to 98%, the effective rate ranges from 92% to 100%, the recurrence rate within three months ranges from 0 to 6%, and the adverse reaction rate is low, thereby proving that the micro-ecological vaginal bacterial agent is effective in treatment of various recurrent vaginitis.

Useful probiotics have been selected among lactic acid bacteria strains of the genus L. acidophilus, L. crispatus, L. gasseri, L. helveticus and L. jensenii for their ability to kill urogenital and / or gastrointestinal pathogens and their ability to inhibit internalization of urogenital and / or gastrointestinal pathogens within urogenital and / or gastrointestinal epithelial cells in humans. Probiotic compositions comprise at least one of the said lactic acid bacteria strains in combination with a suitable delivery system, such as a food product or a beverage, a food or beverage compositions, a food or beverage supplement or adjuvant.

The invention relates to the technical field of food, in particular to a mouth odor refreshing solid drink containing dendrobium officinale prebiotics and probiotics. Dendrobium officinale mannooligosaccharide is added as the prebiotic component, lactobacillus acidophilus, lactobacillus casei, lactobacillus jensenii, lactobacillus Raman, bifidobacterium longum, bifidobacterium breve, bifidobacterium ovale, bifidobacterium thermophilum, lactobacillus rhamnosus, streptococcus thermophilus and yeast are added for compounding to serve as the probiotic component, and then a compound vitamin component is added. The dendrobium officinale mannooligosaccharide and compound vitamin can promote the proliferation of compound probiotic components, rapidly change the structure of oral and intestinal flora, help repair buccal inflammation and damaged gastrointestinal mucosa, regulate the normal functions of the oral cavity, teeth and gastrointestinal tract, solve the causes of halitosis originated from oral factors and halitosis originated from non-oral factors, and can achieve the effect of mouth odor refreshing from the origin. The solid drink can be applied to in toothpaste, mouthwash, functional beverages and other products.

This disclosure relates to a modified version of cyanovirin-N protein and a transformed Lactobacillus bacterium that is capable of recombinantly expressing this modified protein. Further disclosed are polynucleotide sequence encoding the modified cyanovirin-N protein, expression cassette that allows the expression of the modified protein, and method of make and use of the modified protein as well as the transformed Lactobacillus bacterium. In addition, this disclosure describes two novel promoters originated from L. jensenii, which are capable of directing a high level of gene expression in Lactobacillus bacteria. Also described are an expression cassette comprising one of the novel promoters, a genetically modified Lactobacillus bacterium containing the expression cassette, a method for recombinant gene expression in Lactobacillus bacteria using the novel promoters, and a method for delivery of proteins with desired biological activity to a mucosal surface in a human.

The invention discloses a lactobacillus jensenii and application thereof. The lactobacillus jensenii disclosed by the invention is named as IMJ-19, and is collected in the China General Microbiological Culture Collection Center on July 14th, 2014, with the collection number of CGMCC No.9439. The lactobacillus jensenii IMJ-19 has strong capability of generating H2O2, can effectively inhibit the growth of foreign pathogenic bacteria and other conditioned pathogens, also has strong adhesion action to vagina epithelial cells, and ensures the colonization of strains on vaginal epithelial, and the lactobacillus jensenii IMJ-19 has certain application prospects on the aspect of prevention and treatment of urogenital tract infection.

The invention provides lactobacillus jensenii and application thereof for preparing vaginal bacteriostatic drugs. The lactobacillus jensenii is deposited in the China General Microorganisms CollectionCenter of China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No. 14112. Research on the metabolic performance of the lactobacillus jensenii provided by theinvention discovers that, compared to ordinary lactobacillus jensenii, the strain has high lactic acid production capacity, high hydrogen peroxide production capacity, high bacteriostatic ability topathogenic bacteria, and outstanding vaginal epithelial cell adhesion ability. Based on the new properties discovered above, the invention determines a new use of the lactobacillus jensenii for preparing the vaginal bacteriostatic drugs, and the vaginal bacteriostatic drugs can realize treatment of a variety of bacterial vaginal diseases. The bactericidal agent provided by the invention has obvious effects on bacterial vaginal diseases, is safe and non-toxic, has good stability, and can be stored for a long time. The invention also relates to the use of the lactobacillus jensenii in drugs forprevention and / or treatment of gynecological diseases.

The invention discloses active bio-enzyme conditioning extract. The active bio-enzyme conditioning extract comprises, by mass, 14%-16% of lactobacillus acidophilus, 1.35%-15.5% of bifidobacterium longum, 12.5%-14.5% of lactobacillus casei, 11.8%-13.8% of oviform bifidobacteria, 11%-13% of lactobacillus jensenii, 8%-10% of Raman lactobacillus, 6.5%-8.5% of bifidobacterium thermophilum, 1.5%-2.5% of pullulan, 1.5%-2.5% of bacillus subtilis fibrinolytic enzyme, 1.5%-2.5% of lysozyme, 1.5%-2.5% of lysozyme beta-glucan, 1.5%-2.5% of plankton extract, 1.3%-1.7% of bletilla striata root extract, 0.8%-1.2% of scutellaria baicalensis extract, 1%-1.4% of centella asiatica extract, 0.4%-0.6% of licorice extract, 0.7%-0.9% of polygonum cuspidatum extract and 0.3%-0.5% of matricaria extract. The active bio-enzyme conditioning extract is an active bio-enzyme and plant ferment formula developed specially for people with acne-prone and sensitive skin, and is capable of fundamentally improving sensitive, dark and rough skin, removing acne, and recovering white and bright skin.

The invention provides a primer probe combination for detecting female vaginal microorganisms based on quadruple droplet digital PCR and application of the primer probe combination. The primer probe combination comprises a primer pair and a probe, wherein the primer pair and the probe are used for amplifying and detecting a polE gene of inert lactobacillus, a galR gene of lactobacillus jensenii, aCbiQ1 gene of lactobacillus crispatus and a murQ gene of lactobacillus gasseri, wherein the primer pair has relatively high specificity, can avoid primer amplification of other strains during amplification, is used in combination with quadruple droplet digital PCR, classifies and quantitatively analyzes droplets after PCR amplification, and can determine the concentration of each lactobacillus ina sample, determine the dominant microorganisms of the female vagina and realize vagina microorganism typing.

The invention discloses a probiotics syrup capable of improving immunity. Lactobacillus acidophilus, lactobacillus casei, lactobacillus jensenii, lactobacillus plantarum, bifidobacterium longum, bifidobacterium breve, oval bifidobacterium, bifidobacterium bifidum, bifidobacterium catenulatum, bifidobacterium, bacillus acidi lactici, bacterium lacticum, lactic acid bacteria, filiform saccharomycetes, actinomycetes, bifidobacterium thermophilum, gram-positive cocci, streptococcus, galactococcus, intermediary streptococcus, acetobacter aceti, protease, lipase, amylase, cellulose, hemicellulase, pectinase and xylanase are adopted and mixed with traditional Chinese medicine extracts to prepare the probiotics syrup capable of improving immunity. The prepared probiotics syrup can resist pathogenic bacteria, is helpful for cancer treatment and prevention, and can suppress tumor growth and allergic diseases, reduce serum cholesterol and prevent osteoporosis. The probiotics syrup has the advantages of being capable of becoming effective quickly, obvious in effect, high in safety and the like.

The invention discloses a composition composed of three kinds of lactobacillus. The composition contains a lactobacillus rhamnosus strain, a lactobacillus gasseri strain and a lactobacillus jensenii strain, and can resist bacterial vaginosis and related vaginal infection. The composition has a remarkable effect on the bacterial vaginosis and related vaginal diseases, is safe and non-toxic, has good stability, and can be stored for a long time. Compared with other commercially available preparations at present, the strains in the composition can be effectively planted, and the three kinds of lactobacillus strains can play a synergistic role; and compared with a single strain preparation, the composition composed of the three kinds of lactobacillus has the advantage that vaginal microecologycan be formed more obviously.

The invention discloses a preparation for preventing and controlling bee fungal diseases. The preparation consists of 0.1 to 2 percent of (E)-N-(6,6-dimethyl heptanoic-2-olefin-4-alkynyl)-N-methyl-1-naphthalene methylamine hydrochloride, 0.1 to 4 percent of beneficial microorganism and 94 to 99.8 percent of soluble starch or saccharides. The beneficial microorganism comprises one or random combination of bacillus subtilis, bacillus licheniformis, bee bifidobacterium, bifidobacterium asteroids, minimum bifidobacterium, bifidobacterium coryneforme, lactobacillus jensenii, lactobacillus amylovorus, lactobacillus plantarum, tiny lactobacillus and lactobacillus bifermentans; and a gram of the beneficial microorganism comprises 10<9> to 5*10<9> viable bacteria. The preparation for preventing and controlling bee fungal diseases is applied to prevention and control of bee or silkworm fungal diseases.

The invention discloses a microbial preparation and a preparation method thereof, in particular to a hericium erinaceus protein-containing microbial preparation and a preparation method thereof. The hericium erinaceus protein-containing microbial preparation is obtained by uniformly mixing hericium erinaceus protein extract and compound probiotics in the weight ratio ranging from 8:1 to 15:1; thecompound probiotics comprise a mixture of bifidobacteria, lactobacillus acidophilus, lactobacillus casei, lactobacillus jensenii, streptococcus faecalis, galactococcus and intermediate streptococcocciwhich are in the weight ratio of (1 to 2):(1 to 2):(1 to 2):(1 to 2):(1 to 2):(1 to 2):(1 to 2); the activities of the bifidobacteria, the lactobacillus acidophilus, the lactobacillus casei, the lactobacillus jensenii, the streptococcus faecalis, the galactococcus and the intermediate streptococcocci are 5*10<9> to 1*10<10> viable bacteria / g; the preparation method of the hericium erinaceus protein-containing microbial preparation comprises the steps which are shown in the specification; the microbial preparation disclosed by the invention can remarkably improve a gastrointestinal function; compared with using the compound probiotics, for example, the compound probiotics provided by the invention, singly, the microbial preparation has a remarkable synergistic assisting effect.

The invention provides a composite probiotic preparation for targeting treatment on tumor and a preparation method of the composite probiotic preparation. By adopting the technical scheme, on the basis of physiological actions of various probiotics and synergic relationships of the probiotics, bifidobacterium adolescentis, lactobacillus crispatus, lactobacillus jensenii, lactobacillus vaginalis and lactobacillus johnsonii are adopted as preparation main bodies, on the basis, a compounding scheme is designed from angles of amount proportioning, numbers of viable organisms, and the like, and aneffective preparation method is provided. By adopting the preparation, targeting treatment on tumor can be achieved in an oral taking mode, and meanwhile the preparation can have synergetic effects with other anti-tumor medicines to promote anti-tumor effects of the preparation. The preparation is safe and non-toxic, free of conspicuous side effect, free of limits of tumor sizes and types, and good in quantitative treatment effect on various types of tumor.

The invention relates to actobacillus jensenii and application thereof and particularly relates to Lactobacillus jensenii IMJ-118 for preventing and treating bacterial vaginosis. The strain has relatively strong capability of producing H2O2, and can effectively inhibit the growth of external pathogens and other conditional pathogenic bacteria; and meanwhile, the strain has a relatively strong adhesive effect against vaginal epithelial cells and ensures the colonization on vaginal epithelium. Thus, the Lactobacillus jensenii IMJ-118 provided by the invention has certain application prospects in the aspect of preventing and treating bacterial vaginosis.

The invention provides a probiotic preparation for repairing and maintaining microecological balance of the vagina. The probiotic preparation is probiotic powder, and the probiotic powder is composed of lactobacillus plantarum, lactobacillus rhamnosus, lactobacillus acidophilus, lactobacillus jensenii and lactobacillus gasseri. The probiotic preparation provided by the invention has the effects of resisting to bacteria, diminishing inflammation, removing swelling, easing pain, inhibiting bacteria and relieving itching, meanwhile, has the functions of refreshing and removing odor, and can effectively relieve discomfort caused by female vaginitis; meanwhile, the probiotic preparation provided by the invention can also prevent gynecological inflammation, the cervical cancer caused by HIV or HPV and other diseases; and the probiotic preparation provided by the invention is not added with any chemical additive, is natural and healthy, and has no side effect.

The invention relates to a culture medium for culturing a living biological medicine and application of the culture medium. The culture medium for culturing the living biological medicine is prepared from peptone, beef extract, lactulose, mannose oligomer, raffinose, yeast extract, diammonium hydrogen citrate, K2HPO4, MgSO4, MnSO4, Tween-80 and L-cysteine. The living biological medicine comprises any one or a combination of at least two of lactobacillus reuteri, lactobacillus paracasei, lactobacillus acidophilus, lactobacillus rhamnosus, bifidobacterium longum, pediococcus pentosaceus, lactobacillus crispatus, lactobacillus jensenii or lactobacillus gasseri. The culture medium has an unexpected promoting effect on growth, proliferation, metabolism and efficacy exertion of living biological medicines. The efficacy is such as prevention or treatment of gastrointestinal diseases, immune diseases, cancers, vaginitis and other diseases.

Symbiotic bacteria generally mainly producing Lactobacillus bacteria producing H2O2 are distributed on vaginal mucosa of a healthy woman of childbearing age, and the Lactobacillus bacteria functions in protectively preventing urinary and reproductive system infections. Multiple Lactobacillus bacteria are already provided with resistance to, for example, HIV virus by bioengineering so as to prevent the urinary and reproductive system infections. Lactobacillus jensenii producing H2O2, a principle member in the vaginal microbial flora, is reconstructed by bioengineering, so that the Lactobacillus jensenii is enabled to produce and secrete a powerful broad-spectrum antiviral molecule, human IFN Alpha-2b.

Use of an association of lactobacilli for preparation of a pharmaceutical composition for treatment of vaginosis and vaginitis. Said bacteria association comprises the Lactobacillus brevis and Lactobacillus salivarius subs. salicinius species, possibly in combination with one or more species selected from Lactobacillus salivarius subs. salivarius, Lactobacillus jensenii, Lactobacillus catenaforme, Lactobacillus minutus and Lactobacillus gasseri.A pharmaceutical composition comprising said association of lactobacilli adapted for treatment of vaginosis and vaginitis.A method of treating vaginosis and vaginitis based on administration of said pharmaceutical composition.

The invention discloses a high-glucose-resistant lactic acid production strain and application thereof in D-lactic acid production. The invention provides bacillus coagulans G35, and the preservation number of the bacillus coagulans G35 in the China General Microbiological Culture Collection Center is CGMCC (China General Microbiological Culture Collection Center) No. 21293. The invention further provides the bacillus coagulans G35-LjLDH, and the bacillus coagulans G35-LjLDH is a recombinant strain obtained by replacing a D-lactic dehydrogenase gene of a strain G35 with a D-lactic dehydrogenase gene from lactobacillus jensenii. The strain developed and obtained by the invention has the characteristics of high glucose tolerance, capability of utilizing a low-concentration organic nitrogen source, high temperature resistance and high D-lactic acid yield, and has important practical significance.

The invention discloses a primer probe combination and a kit for vaginal micro-ecology detection, the primer probe combination comprises target gene detection primers and probes for lactobacillus crispatus (LC), lactobacillus gasseri (LG), lactobacillus jensenii (LJ), lactobacillus inertus (LI), gardnerella vaginalis (GV), candida (CA) and trichomonas vaginalis (TV), and the probes are used for melting curve typing. The kit comprises a primer probe combination. Through combination of the specific primers and the probes, specific target genes of different microorganisms can be effectively amplified, melting curve analysis can be performed on amplification products, the seven microorganisms can be detected in one tube, and the method has the advantages of high specificity, short time consumption, high sensitivity, comprehensive detection site coverage and the like; auxiliary diagnosis reference can be provided for clinicians, treatment can be carried out in advance, and clinical application is wide.

The invention discloses lactobacillus jensenii and application thereof. The lactobacillus jensenii is particularly lactobacillus jensenii IMJ-1, and the file No. of the lactobacillus jensenii IMJ-1 in the General Microbiological Culture Collection Center of China Microbial Culture Collection Management Committee is CGMCC No. 6558. The lactobacillus jensenii IMJ-1 CGMCC No.6558 has high H2O2 generation capability, can effectively suppress breeding of external pathogenic bacteria and other conditional pathogenic bacteria, and has a high binding function to epithelial cells of vagina, and accordingly, breeding of bacteria strains on the vaginal epithelium is guranteed. Besides, the lactobacillus jensenii IMJ-1 CGMCC No. 6558 has a certain application prospect in preventing and curing bacterial vaginosis.

The invention discloses a strain of Lactobacillus jansii and application thereof. Lactobacillus jensenii (Lactobacillus.jensenii) disclosed by the present invention is named as IMJ‑19, and has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on July 14, 2014, and its preservation number is CGMCC No.9439 . Lactobacillus jensenii (Lactobacillus.jensenii) IMJ‑19 disclosed by the invention has a strong ability to produce H2O2, can effectively inhibit the growth of foreign pathogenic bacteria and other conditional pathogenic bacteria, and has a strong effect on the vaginal epithelium. The adhesion of the cells ensures the colonization of the strain on the vaginal epithelium, and Lactobacillus jensii IMJ‑19 has a certain application prospect in the prevention and treatment of urogenital tract infections.

The invention relates to poplar cutting nutritional soil. The poplar cutting nutritional soil comprises, by weight, field soil, compound fertilizers, zeolite, activated carbon, soybeans, egg white, lactobacillus jensenii solution, saccharomyces cerevisiae solution, radix codonopsis, radix astragali, fructus lycii, lemons, honey, vitamin C, taurine, calcium magnesium phosphate fertilizers, ammonium superphosphate, aspartic acid, glutamic acid and water. The poplar cutting nutritional soil has the advantages that the water retention and fertilizer conservation capacity of the field soil, the activated carbon and the zeolite is utilized, the field soil, components of the poplar cutting nutritional soil are arranged in a layered manner, and accordingly nutrient sustained-release effects can be realized; nutrition can be assuredly directly supplied to roots in integral cutting rooting procedures, accordingly, the survival rate of cutting can be greatly increased, obvious effects can be realized, the survival rate can be increased from the conventional survival rate of 67.6% and is higher than 82.4% at least as proved by experiments, and the poplar cutting nutritional soil is worthy of being popularized and utilized.

The present invention relates to a kind of microecological preparation and preparation method thereof, especially to a kind of microecological preparation containing Morinda officinalis fructooligosaccharide and preparation method thereof, said method comprises: Morinda officinalis fructooligosaccharide and compound probiotic Obtained by mixing evenly at a weight ratio of 8:1-15:1, the composite probiotics contain a weight ratio of 1-2:1-2:1-2:1-2:1-2:1-2:1-2 Mixture of Bifidobacterium, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus jansneri, Streptococcus faecalis, Lactococcus, Streptococcus intermedius. The above-mentioned microecological preparation of the present invention can significantly improve gastrointestinal function, and has a significant synergistic auxiliary effect compared with the simple use of compound probiotics such as the compound probiotics described in the present invention.

The invention discloses lactobacillus jensenii for relieving inflammation caused by gardnerella vaginalis and application of the lactobacillus jensenii, and belongs to the technical field of microorganisms. The lactobacillus jensenii is screened from the vagina of a healthy woman, and the lactobacillus jensenii can remarkably relieve and improve bacterial vaginitis caused by gardnerella, regulate the level of inflammatory factors, relieve the condition that vaginal epithelial cells fall off and improve the recovery capacity of vaginal tissue. The lactobacillus jensenii can be used for preparing products for relieving bacterial vaginitis and has a wide application prospect.

The invention relates to Lactobacillus jensenii and application thereof, in particular to Lactobacillus jensenii IMJ‑118 used for preventing and treating bacterial vaginosis. The strain has a strong production of H 2 o 2 The ability to effectively inhibit the growth of foreign pathogens and other conditional pathogens; at the same time, it has a strong adhesion to vaginal epithelial cells, ensuring the colonization of bacterial strains in the vaginal epithelium. Therefore, the Lactobacillus jansii IMJ‑118 provided by the present invention has a certain application prospect in the prevention and treatment of bacterial vaginosis.

The invention relates to a microbial ecological agent and a preparation method thereof, in particular to a microbial ecological agent containing radix morindae officinalis fructo-oligosaccharide, andthe preparation method thereof. The method comprises the step of uniformly mixing the radix morindae officinalis fructo-oligosaccharide and compound probiotics according to the weight ratio of (8 to1)to (15 to 1), wherein the compound probiotics is a mixture prepared from bifidobacterium, lactobacillus acidophilus, lactobacillus casei, lactobacillus jensenii, streptococcus faecium, galactococcusand intermediate streptococcus with the weight ratio being (1 to 2) to (1 to 2) to (1 to 2) to (1 to 2) to (1 to 2) to (1 to 2) to (1 to 2). The microbial ecological agent provided by the invention can be beneficial to remarkably improving a gastrointestinal function, and has a remarkable synergistic assistance effect compared with a way of independently using the compound probiotics such as the compound probiotics provided by the invention.