44 results about "Yersinia" patented technology

The invention belongs to the technical field of biology, and discloses a yarrowia lipolytica engineering strain for efficiently producing eicosapentaenoic acid based on citric acid metabolism regulation, a method and application of the yarrowia lipolytica engineering strain. Delta9 elongase, Delta8 desaturase, Delta5 desaturase and Delta17 desaturase genes in a fatty acid synthesis route, heterologous ATP-citrate lyase and a mitochondrial citric acid vector are overexpressed. According to the invention, ATP-citric acid lyase and a mitochondrial citric acid carrier gene which are respectively derived from Schizochylene sp. And Saccharomyces cerevisiae are jointly expressed in the yarrowia lipolytica engineering strain, so that the yields of EPA and grease in the yarrowia lipolytica engineering strain are remarkably improved, and compared with a control strain, the yields of the grease and the EPA are respectively improved by 243.35% and 161.85%.

The invention discloses a kit for simultaneously detecting 16 food-borne pathogenic bacteria. Each group of probes is designed based on bacterial classification, virulence gene specificity or serotype marks of the pathogenic bacteria; a group A probe vibrio detection group is used for detecting vibrio cholerae universal type, vibrio cholerae O1 serotype, vibrio cholerae O139 serotype and vibrio parahaemolyticus; the B group of probe invasive enterobacteriaceae pathogen group is used for detecting escherichia coli O157: H7, shigella, salmonella and yersinia small intestine; the C group probe escherichia coli pathotype detection group is used for detecting enterotoxigenic escherichia coli ETEC, enteroinvasive escherichia coli EIEC, enteroaggregative adherent escherichia coli EAEC and pathogenic escherichia coli EPEC; and the D group of probes are used for detecting campylobacter jejuni, campylobacter colons, listeria monocytogenes and enterohemorrhagic escherichia coli EHEC in the campylobacter and toxin type pathogen group.

The invention discloses a primer and a kit for rapidly detecting yersinia rouxii through RPA-CRISPR / Cas12a and application of the primer and the kit, and relates to the technical field of disease prevention and control in aquaculture. The invention aims to solve the problems that the existing detection method is time-consuming and labor-consuming, is easy to cause pollution and needs to be matched with professional detection equipment. According to the present invention, the RPA amplification primer is composed of a forward primer yrp1-RPA-F2 and a reverse primer yrp1-RPA-R2, the crRNA is yrp1-crRNA-R1, and the kit for rapid detection of yersinia rouxii contains the RPA amplification primer and the crRNA. The method for rapidly detecting yersinia rouxii comprises a fluorescence method and a test paper method. The method disclosed by the invention has the advantages of good inclusiveness, strong specificity, high sensitivity and the like, detection is carried out based on the RPA primer and the crRNA, and the obtained RPA product is detected by using a lateral flow chromatography test strip and fluorescence, so that the purpose of portable visual rapid detection on site or in a breeding base is achieved, and the method is suitable for large-scale popularization and application. The method has very high clinical application value in the aspects of early discovery of diseases, epidemiological investigation and comprehensive prevention and control.

This invention discloses a lipophilic yeast strain for synthesizing astaxanthin (Yersinia lipophila). Yarrowia lipolytica This invention relates to an engineered strain, its construction method, and its application. The engineered strain is a *Yersinia lipolytica* strain carrying CrtZ and CrtW. The invention utilizes genetic engineering methods to introduce a *Yersinia lipolytica* strain derived from *Rhodochophora* into a β-carotene-producing strain via genetic engineering. Haematococcus Pluvialis The β-carotene hydroxylase encoding gene (HpCrtZ) originates from the genus Paracoccus ( Paracoccus sp. The gene encoding β-carotene ketolase (PsCrtW) and the gene derived from Saccharomyces cerevisiae (Saccharomyces cerevisiae) Saccharomyces cerevisiae The method involves obtaining a genetically engineered *Yersinia lipolytica* strain that produces astaxanthin by constructing a fusion enzyme complex and fusing it with peroxisomes, endoplasmic reticulum, and lipid droplet-targeting signal peptides to obtain a *Yersinia lipolytica* strain with higher astaxanthin yield. The astaxanthin-producing genetically engineered strain constructed in this invention achieved an astaxanthin yield of 1.43 g / L in a 5 L bioreactor after pH control and continuous feeding optimization.

The invention relates to a primer and probe combination, a kit and a method for detecting gastrointestinal tract pathogenic bacteria. The gastrointestinal pathogenic bacteria comprise vibrio cholerae, vibrio parahaemolyticus, salmonella, shigella, diarrheagenic escherichia coli, campylobacter, yersinia enterocolons, clostridium difficile, cronobacter sakazakii, aeromonas hydrophila, plesiomonas shigelloides and vibrio fluvialis. The primer and the probe group can be independently used for PCR amplification detection and can be combined in the same reaction system and reaction procedure for multiple PCR amplification detection, and the primers and the probes aiming at different pathogenic bacteria cannot interfere with each other during multiple PCR amplification detection. The primer and probe combination covers various types of gastrointestinal pathogenic bacteria, and is high in sensitivity, low in detection limit, good in specificity and wide in application prospect.

This invention relates to the fields of synthetic biology and biochemical engineering, and provides an engineered *Yersinia lipolytica* strain that enhances coumaric acid accumulation, its construction method, and its applications. The engineered *Yersinia lipolytica* strain of this invention uses *Yersinia lipolytica* as the substrate strain, expresses the tyrosine ammonia-lyase encoding gene RgTAL derived from *Rhodotorula glutinis*, and completely knocks out the aminotransferase encoding gene containing the nucleotide sequence shown in SEQ ID NO 1 (database number YALI0A08734g). RgTAL converts endogenous L-tyrosine to coumaric acid. This invention reduces dependence on complex metabolic regulation and increases the coumaric acid accumulation level in *Yersinia lipolytica*.

The invention discloses yarrowia lipolytica engineering bacteria for producing esterified astaxanthin as well as a construction method and application of the yarrowia lipolytica engineering bacteria. According to the invention, acyltransferase ScDGAT2-1 from fission vibrio is integrated in a genome of a yarrowia lipolytica engineering strain DN20, diacylglycerol acyltransferase DGA1 and phosphatidic acid phosphatase PAH1 are knocked out, and the corn oil lipoprotein Oleosin and the acyltransferase ScDGAT2-1 from fission vibrio are subjected to fusion expression, so that the yarrowia lipolytica engineering strain DN20 is obtained. According to the present invention, the Yarrowia lipolytica engineering bacterium capable of producing esterified astaxanthin at a high yield is constructed by using SBT06-005 as a raw material and integrating beta-carotene hydroxylase CrtZ, endogenous truncated 3-hydroxy3-methylglutaryl coenzyme A reductase tHMG1, phytoene synthase CarRP and beta-isopropyl malate dehydrogenase LEU2, and the SBT06-005 can produce 166.6 mg / L esterified astaxanthin and 1097.0 mg / L total astaxanthin in a 5L fermentation tank.

The invention relates to a Brucella lipopolysaccharide monoclonal antibody and a cELISA detection kit and detection method based on the same, the monoclonal antibody comprises a heavy chain variable region and a light chain variable region, and is characterized in that the amino acid sequence of the heavy chain variable region is SEQ ID No.1, and the corresponding nucleotide sequence is SEQ ID No.2; the amino acid sequence of the light chain variable region is SEQ ID No.3, and the corresponding nucleotide sequence is SEQ ID No.4. The monoclonal antibody is only specifically combined with smooth LPS of brucella, and has no cross reaction with 21 pathogens such as rough brucella, yersinia O: 9 and the like. The sensitivity of a competitive enzyme-linked immunosorbent assay (cELISA) kit developed on the basis of the antibody reaches 1: 25600 and is improved by 200 times compared with that of a traditional method, the detection time is shortened to 90 min, the in-plate / inter-plate variable coefficient is lower than 9.87%, and the antibody is suitable for high-throughput screening of multi-host serum.

The present invention relates to a recombinant protein and polypeptide with antibacterial properties derived from the gene YR7 from Yersinia ruckeri, compositions comprising them, uses and the method utilizing thereof. The recombinant proteins and the polypeptide with antibacterial properties will be used to prevent the development of bacteria in aquatic environments and will have uses in the field of zootechnics, fishery and fish farming, in particular - of salmonid fish in aquacultures, especially against Yersinia ruckeri causing fish yersiniosis.

The invention discloses a cell-penetrating peptide CPP137 as well as a compound, a composition and application thereof, and belongs to the technical field of polypeptides. According to the application, a cell-penetrating peptide CPP137 with immune cell selectivity is screened out from a plague bacillus sORF library. The polypeptide not only can be efficiently internalized by THP-1 (M0 type) mononuclear cells, but also can specifically target two key antigen presenting cells, namely primary macrophages and dendritic cells (DC), in a complex human whole blood physiological environment, but is not obviously combined with other blood cell types. Therefore, the cell-penetrating peptide CPP137 is a targeted delivery carrier with great potential, can be used for specifically delivering a therapeutic load to myeloid immune cells, and is used for treating intracellular infection and immune-related diseases or used as a vaccine development platform. Meanwhile, CPP137 can also be used as a specific diagnostic marker or an imaging probe for detecting or tracing pathological states related to macrophages / DC.

The present invention relates to the technical field of Brucella detection. The present invention provides a Brucella-specific antigen and its application. The nucleotide sequence of the Brucella-specific antigen is shown in SEQ ID NO.1. The Brucella-specific antigen of the present invention has strong specificity and good use effect. A test strip prepared using the antigen of the present invention can still detect a significant positive reaction when the positive serum concentration is diluted to 1:40; the test strip of the present invention detects Yersinia intestinalis-positive serum and Escherichia coli-positive serum, and the results are both negative, indicating that the test strip has good specificity.

The invention discloses application of a substance for inhibiting Yp1842 protein expression quantity and / or activity in reducing yersinia pestis virulence. The amino acid sequence of the Yp1842 protein is as shown in SEQ ID NO: 2. 100CFU plague bacillus yp1842 and 201 plague bacillus strains are subcutaneously injected into the groin of the Bal b / c mouse, and then the mouse is normally fed for 14 days; the survival condition of the mouse is observed during the period. The result shows that compared with 201 strains of plague bacillus, the toxicity of the plague bacillus yp1842 is obviously reduced. Therefore, the Yp1842 protein has an important influence on the virulence of the yersinia pestis. The method has great application value.

This invention provides a genetically engineered *Yersinia lipolytica* strain and its applications. The genetically engineered strain is a *Yersinia lipolytica* Po1f strain with the PEX10 gene knocked out, expressing the C16 / 18 elongase gene MaELO3 from *Morchella alpina*, the elongase gene AtKCS from *Arabidopsis thaliana*, and the elongase gene CraKCS from *Brassica oleracea*. The strain also expresses the elongase gene CgKCS from *Capsella*. Further expression of the desaturase gene MaD15D from *Morchella alpina*, and overexpression of the strain's own diacylglycerol transferase gene DGA1 and its own Δ9 desaturase gene OLE1, resulted in a nervonic acid yield of 111.6 mg / L in shake-flask fermentation. By adding 1% rapeseed oil exogenously, the nervonic acid yield reached 185.1 mg / L. The *Yersinia lipolytica* genetically engineered strain constructed in this invention is simple to operate, has stable and reliable performance, and can be applied to large-scale commercial production.

The invention provides a recombinant lipolytic yeast strain with high yield of sclareol as well as a preparation method and application of the recombinant lipolytic yeast strain. The recombinant yarrowia lipolytica strain can produce sclareol with high yield and efficiency, and effectively solves the problem of low yield of sclareol prepared by fermentation of existing recombinant yarrowia lipolytica. Meanwhile, when the recombinant lipolytic yeast strain is used for producing sclareol, the stability among batches is relatively good, and the requirements of large-scale industrial production on product stability can be met. Therefore, the recombinant lipolytic yeast strain disclosed by the invention has relatively high production benefits in the production of sclareol, so that the recombinant lipolytic yeast strain has a relatively good industrial application prospect.

The inventors found in a sage, Salvia pisidica Boiss. & Heldr. substances that can kill Borrelia spp. and Yersinia spp. in vitro and in vivo. Up to now, it has been assumed that a methanol extract or the oil of Silvia pisidica has antimicrobial effects against gram (+) bacteria. Previous experiments confirm this current scientific view.

We have shown that cprA expression leads to the production of outer membrane vesicles (OMVs) that block autophagic flux and have a greater capacity to activate the non-canonical inflammasome pathway. In a murine model of sepsis, a P. aeruginosa strain deleted for cprA was less virulent than the wild-type strain. These results demonstrate the important role of CprA in the pathogenicity of P. aeruginosa. It is worth noting that CprA is also a functional ortholog of HlyF, which is encoded by virulence plasmids of Escherichia coli. We have shown that other cryptic SDRs encoded by mammalian and plant pathogens, such as Yersinia pestis and Ralstonia solanacearum are functional orthologs of CprA and HlyF. These SDRs also induce the production of OMVs which block autophagic flux. This study uncovers a new family of virulence determinants in Gram-negative bacteria, offering potential for innovative therapeutic interventions and deeper insights into bacterial pathogenesis. The present invention relates to a method for producing fatty alcohols in a medium comprising HlyF protein, or an ortholog of HlyF protein, and fatty acyl-CoA.

This invention relates to a method for improving tobacco stem extract using multi-strain synergistic fermentation and its application. The method includes: (1) inoculating Bacillus subtilis and Bacillus kochia into a culture medium containing tobacco stem extract for fermentation to obtain a primary fermentation broth; (2) inoculating Saccharomyces cerevisiae (producing phenylethanol) and Yersinia geraniolata (producing geraniol lipolysin) into the primary fermentation broth for further fermentation to obtain an improved tobacco stem extract. This invention creatively provides a method for improving tobacco stem extract using multi-microorganism synergistic fermentation. This method effectively degrades macromolecular components such as cellulose, hemicellulose, pectin, and starch in the tobacco stem extract while increasing the content of aroma components in the tobacco stem extract. This significantly reduces the off-flavors and irritation of the treated improved tobacco stem extract, improves its sensory quality, and significantly enhances its reclaiming value.

A method for improving the synthetic ability of *Yersinia lipolytica* to synthesize amino acid derivatives. This invention discloses an engineered strain for the biological synthesis of the amino acid derivative β-hydroxy-β-methylbutyric acid (β-methylbutyric acid) and its construction method. Using *Yersinia lipolytica* as the substrate cell, the HMGCL and OCT genes involved in branched-chain amino acid metabolism in its genome are knocked out to obtain a gene knockout strain. Decarboxylase genes, hydratase genes, dehydratase genes, and thioesterase genes are integrated into the genome of the gene knockout strain to obtain an engineered strain capable of de novo synthesis of β-hydroxy-β-methylbutyric acid. Further integration of the CcGCTB-CcGCTA fusion gene expression module enhances the expression level of key enzyme genes, thereby increasing the yield of β-hydroxy-β-methylbutyric acid in the strain. The engineered strain constructed in this invention can synthesize the target product β-hydroxy-β-methylbutyric acid using glucose as a carbon source through fermentation, with a maximum yield of up to 30 g / L.

The invention discloses a primer probe composition, a kit and a method for detecting 16 pathogenic microorganisms. The 16 pathogenic microorganisms comprise total bacterial colonies, total vibrio, helicobacter pylori, aeromonas hydrophila, mycobacterium tuberculosis, vibrio cholerae, yersinia pestis, pseudomonas aeruginosa, staphylococcus aureus, mycoplasma, chlamydia, rickettsia and toxoplasma gondii. The sequences of screened primer probes are shown as SEQ ID NO.1-SEQ ID NO.48, the sensitivity is highest, the specificity is good, the primer probes serve as a quadruple detection reagent, an optimized lysis solution and a fluorescent PCR amplification program are combined, the extraction effect and the sensitivity are guaranteed, the method is combined with a nucleic acid extraction and detection integrated device, the detection efficiency is high, and the detection cost is low. Full automation is achieved from nucleic acid extraction to qPCR detection, only 60 min is needed from extraction to qPCR detection, and the detection time is shortened.

The invention discloses a primer group for simultaneously detecting seven pet enteropathogenic bacteria and application thereof, the primer group comprises primer sequences as shown in SEQ ID NO: 1-14, and the seven pet enteropathogenic bacteria are hemorrhagic escherichia coli, salmonella enteritidis, campylobacter jejuni, clostridium difficile, clostridium perfringens, yersinia enteritidis and staphylococcus aureus. Extracting DNA (Deoxyribonucleic Acid) of a sample to be detected by using the excrement sample nucleic acid extraction kit; taking the sample DNA as a template, carrying out multiple PCR reaction by using the primer group to obtain an amplification curve, and if the Ct value of the amplification curve of the sample to be detected is 0 amp, detecting the Ct value of the sample to be detected; lt; c < tamp >; lt; 30, and the Ct value of the IPC is amp; lt; and 40, determining that the interpretation result is positive, and indicating that the sample to be detected contains the one or more enteropathogenic bacteria. According to the invention, a primer sequence with high sensitivity and specificity is adopted, so that the quality of a detection result is ensured; the detection method is simple to operate, time-saving and labor-saving; the detection flux is high, and the reagent consumable cost is low.

This invention discloses an engineered strain of *Yersinia lipolytica* for synthesizing astaxanthin, its preparation method and application, a method for producing astaxanthin, and a method for increasing astaxanthin yield. The engineered strain of *Yersinia lipolytica* for synthesizing astaxanthin is obtained by expressing phytoene dehydrogenase CarB, phytoene synthase / lycopene cyclase CarRP, β-carotene ketolase CrtW, β-carotene hydroxylase CrtZ, and ferricoxane reductase RFNR in a host strain. RFNR Derived from Chlamydomonas rhinelandii Chlamydomonas reinhardtii Haematococcus pluvialis Haematococcus pluvialis This strain, through heterologous expression of RFNR, efficiently provides reducing power to CrtZ, significantly enhancing the catalytic activity of CrtZ and ultimately achieving higher astaxanthin yield.

The invention discloses recombinant yersinia pestis with reduced toxicity. The recombinant yersinia pestis is obtained by inhibiting the expression quantity and / or activity of Yp1841 protein in the yersinia pestis; the amino acid sequence of the Yp1841 protein is as shown in SEQ ID NO: 2. 100CFU plague bacillus yp1841 and 201 plague bacillus strains are subcutaneously injected into the groin of the Bal b / c mouse respectively, and then the mouse is normally fed for 14 days; the survival condition of the mouse is observed during the period. The result shows that compared with 201 strains of plague bacillus, the toxicity of the plague bacillus yp1841 is obviously reduced. Therefore, the Yp1841 protein has an important influence on the virulence of the yersinia pestis. The method has great application value.

The invention belongs to the field of life science, and particularly relates to a yersinia quorum sensing system research method, whole-cell biosensor construction based on the method and application of the whole-cell biosensor. The method comprises the following steps: (1) constructing knockout bacteria and YpsR back-supplementing bacteria of a yersinia pseudotuberculosis quorum sensing system YpsR / YpsI; (2) analysis and screening of the affinity of an N-acyl homoserine lactone (AHL) signal small molecule and a YpsR (Yttrium limacinum) regulatory protein, and (3) analysis and screening of the affinity of the N-acyl homoserine lactone (AHL) signal small molecule and the YpsR regulatory protein; (3) construction of a gene line based on AHL and YpsR regulatory protein; and (4) functional verification of the Y.pstb whole-cell sensor based on AHL detection. The biosensor capable of detecting the AHL generated by the yersinia pseudotuberculosis is constructed by researching a YpsR / YpsI quorum sensing system and a molecular signal recognition and combination mechanism, and more theoretical basis and technical support are provided for prevention, control and treatment of the yersinia pseudotuberculosis.

The invention relates to a protein delivery system, a preparation method and modification and application thereof, and belongs to the technical field of biology. The protein delivery system disclosed by the invention comprises a structural protein derived from a Yersinia similia 228 strain, a regulatory factor derived from a Photorhabus asymbiosis 43949 strain, and a signal peptide derived from a pathogenic bacterium, a yersinia and a non-symbiotic photobacterium, and is characterized in that the protein delivery system comprises the structural protein derived from the Yersinia similia 228 strain and the regulatory factor derived from the Photorhabus asymbiosis 43949 strain. The protein delivery system disclosed by the invention can be expressed in escherichia coli and assembled into the YPCIS protein compound, and the effect protein can be loaded to the YPCIS protein compound through the signal peptide; on the basis, the recognition region of the protein coded by the Cis gene cluster is further subjected to adaptive transformation, so that the YPCIS protein compound can target a target cell, and the specific delivery of the effect protein is realized.

This invention discloses a thermophilic recombinant *Yersinia lipolytica* strain producing carotenoids, its construction method, and its applications. The recombinant strain exogenously expresses thermophilic precursor fatty acid desaturase (FAD3) and isocitrate dehydrogenase (IDH2) mutants from *Rhodotorula rubrum*, achieving efficient synthesis at 30°C. Secondly, the metabolic pathway of the recombinant strain is optimized based on a metabolic flux push-pull strategy, overexpressing isopentenyl pyrophosphate isomerase (IDI), farnesyl pyrophosphate synthase (ERG20), phytene dehydrogenase (CarB), and bifunctional phytene synthase (CarRP) to increase precursor throughput. Finally, multiple copies of the genes for astaxanthin synthase (crtS) and cytochrome P450 reductase (crtR) from *Rhodotorula rubrum* are performed to further enhance the carotenoid production capacity of *Yersinia lipolytica*. The thermophilic *Yersinia lipolytica* construction method of this invention is simple, can achieve more efficient carotenoid synthesis at higher temperatures, significantly reduces fermentation costs, and has potential for industrial production.

The application discloses a diagnostic antigen for quantitatively detecting Yersinia pestis antibody. Specifically disclosed is a protein F1B139 with an amino acid sequence of SEQ ID No. 1 and application of the protein F1B139 as an antigen in detection, analysis or evaluation of Yersinia pestis antibody. Experiments show that the Yersinia pestis antigen has good sensitivity, can be recognized by rabbit anti-F1 polyclonal antibody, can be used as a quality control product of a Yersinia pestis antigen detection reagent, ensures batch stability of antibody raw material production, can be used as a raw material of an antibody detection reagent, the recombinant F1 antigen component is single, and the antibody can be accurately evaluated. The immunodetection method and product based on the antigen can realize quantitative detection of the antibody, have good sensitivity, can be widely used in detection, quality control and evaluation of the Yersinia pestis antibody, provide help for clinical diagnosis, curative effect observation, prognosis judgment, epidemiological investigation of infectious diseases and the like, and have very wide clinical application prospects.

Biopolymer compositions comprising antimicrobial peptides (AMPs) for treating infections such as bacterial infections, viral infections, fungal infections, and parasitic infections. The compositions herein may also be used for treating infections associated with antibiotic-resistant bacteria, antifungal-resistant fungi, antiviral-resistant viruses, or for treating biological warfare agents (BWAs) such as Bacillus anthracis and Yersenia pestis. The present invention also provides methods of synthesis of said biopolymer compositions, wherein AMP biopolymers can be synthesized as an artificially engineered protein by genetically fusing an AMP; a protein that behaves similarly to polymer tethers; and a protein as a modifiable material platform that can transform to self-assembled nanoparticles, self-standing films, or adhesives to easily attach tethered AMPs onto any biomaterial surface for various clinical applications.

A vaccine platform using a Yersinia pestis mutant synthesizing an adjuvant form lipid A (monophosphoryl lipid A, MPLA) for the increased biogenesis of bacterial outer membrane vesicles (OMVs). To enhance the immunogenicity of the OMVs, an Asd-based balanced-lethal host-vector system was constructed to oversynthesize the LcrV antigen of Y. pestis, raise the amounts of LcrV enclosed in OMVs by Type II secretion system, and eliminate harmful factors like plasminogen activator (Pla) and murine toxin from the OMVs. Vaccination with OMVs containing MPLA and increased amounts of LcrV with diminished toxicity afforded complete protection in mice against subcutaneous challenge and intranasal challenge and was significantly superior to that resulting from vaccination with LcrV / alhydrogel. Additionally, the Yersinia OMV can be used as a platform to deliver the heterologous antigens of Bacillus anthraces. Vaccination with multiantigenic self-adjuvanting bionanoparticles from Pseudomonas was also successfully tested in connection with Pseudomonas aeruginosa.

This invention provides a recombinant lipolytic yeast strain with high perillaldehyde production, its preparation method, and its applications. This recombinant lipolytic yeast strain can produce perillaldehyde with high yield and efficiency, effectively solving the problem of low yield in existing recombinant Yersinia lipolytic yeast fermentation for perillaldehyde production. Furthermore, the batch-to-batch stability of perillaldehyde production using this recombinant lipolytic yeast strain is good, meeting the requirements for product stability in large-scale industrial production. Therefore, the recombinant lipolytic yeast strain of this application has high production efficiency in perillaldehyde production, thus showing good prospects for industrial application.

This invention provides a composition, kit, and application for triple real-time fluorescence PCR detection of pathogenic Yersinia pestis based on TaqMan probes. Specific primers and TaqMan probes are designed targeting the caf1 gene of Yersinia pestis, the foxA gene of Yersinia enterocolitica, and the inv gene of Yersinia pseudotuberculosis, enabling simultaneous detection of these three pathogens in a single reaction system. The optimized annealing temperature is 54℃, and the final probe concentration is 0.3 μM. This method exhibits good linearity (R²>0.99), with detection limits of 5×10² copies / μL for I9 and C4, and 1×10¹ copies / μL for F4. Intra-assay and inter-assay coefficients of variation are mostly below 2%, and there is no cross-reactivity with non-target bacteria. It demonstrates good sensitivity, specificity, and repeatability, and can be used for rapid detection of pathogenic Yersinia pestis in food, environmental, clinical, and animal-derived samples.