15 results about "Yersinia" patented technology

The invention discloses a primer and a kit for rapidly detecting yersinia rouxii through RPA-CRISPR / Cas12a and application of the primer and the kit, and relates to the technical field of disease prevention and control in aquaculture. The invention aims to solve the problems that the existing detection method is time-consuming and labor-consuming, is easy to cause pollution and needs to be matched with professional detection equipment. According to the present invention, the RPA amplification primer is composed of a forward primer yrp1-RPA-F2 and a reverse primer yrp1-RPA-R2, the crRNA is yrp1-crRNA-R1, and the kit for rapid detection of yersinia rouxii contains the RPA amplification primer and the crRNA. The method for rapidly detecting yersinia rouxii comprises a fluorescence method and a test paper method. The method disclosed by the invention has the advantages of good inclusiveness, strong specificity, high sensitivity and the like, detection is carried out based on the RPA primer and the crRNA, and the obtained RPA product is detected by using a lateral flow chromatography test strip and fluorescence, so that the purpose of portable visual rapid detection on site or in a breeding base is achieved, and the method is suitable for large-scale popularization and application. The method has very high clinical application value in the aspects of early discovery of diseases, epidemiological investigation and comprehensive prevention and control.

This invention discloses a lipophilic yeast strain for synthesizing astaxanthin (Yersinia lipophila). Yarrowia lipolytica This invention relates to an engineered strain, its construction method, and its application. The engineered strain is a *Yersinia lipolytica* strain carrying CrtZ and CrtW. The invention utilizes genetic engineering methods to introduce a *Yersinia lipolytica* strain derived from *Rhodochophora* into a β-carotene-producing strain via genetic engineering. Haematococcus Pluvialis The β-carotene hydroxylase encoding gene (HpCrtZ) originates from the genus Paracoccus ( Paracoccus sp. The gene encoding β-carotene ketolase (PsCrtW) and the gene derived from Saccharomyces cerevisiae (Saccharomyces cerevisiae) Saccharomyces cerevisiae The method involves obtaining a genetically engineered *Yersinia lipolytica* strain that produces astaxanthin by constructing a fusion enzyme complex and fusing it with peroxisomes, endoplasmic reticulum, and lipid droplet-targeting signal peptides to obtain a *Yersinia lipolytica* strain with higher astaxanthin yield. The astaxanthin-producing genetically engineered strain constructed in this invention achieved an astaxanthin yield of 1.43 g / L in a 5 L bioreactor after pH control and continuous feeding optimization.

This invention relates to the fields of synthetic biology and biochemical engineering, and provides an engineered *Yersinia lipolytica* strain that enhances coumaric acid accumulation, its construction method, and its applications. The engineered *Yersinia lipolytica* strain of this invention uses *Yersinia lipolytica* as the substrate strain, expresses the tyrosine ammonia-lyase encoding gene RgTAL derived from *Rhodotorula glutinis*, and completely knocks out the aminotransferase encoding gene containing the nucleotide sequence shown in SEQ ID NO 1 (database number YALI0A08734g). RgTAL converts endogenous L-tyrosine to coumaric acid. This invention reduces dependence on complex metabolic regulation and increases the coumaric acid accumulation level in *Yersinia lipolytica*.

We have shown that cprA expression leads to the production of outer membrane vesicles (OMVs) that block autophagic flux and have a greater capacity to activate the non-canonical inflammasome pathway. In a murine model of sepsis, a P. aeruginosa strain deleted for cprA was less virulent than the wild-type strain. These results demonstrate the important role of CprA in the pathogenicity of P. aeruginosa. It is worth noting that CprA is also a functional ortholog of HlyF, which is encoded by virulence plasmids of Escherichia coli. We have shown that other cryptic SDRs encoded by mammalian and plant pathogens, such as Yersinia pestis and Ralstonia solanacearum are functional orthologs of CprA and HlyF. These SDRs also induce the production of OMVs which block autophagic flux. This study uncovers a new family of virulence determinants in Gram-negative bacteria, offering potential for innovative therapeutic interventions and deeper insights into bacterial pathogenesis. The present invention relates to a method for producing fatty alcohols in a medium comprising HlyF protein, or an ortholog of HlyF protein, and fatty acyl-CoA.

This invention relates to a method for improving tobacco stem extract using multi-strain synergistic fermentation and its application. The method includes: (1) inoculating Bacillus subtilis and Bacillus kochia into a culture medium containing tobacco stem extract for fermentation to obtain a primary fermentation broth; (2) inoculating Saccharomyces cerevisiae (producing phenylethanol) and Yersinia geraniolata (producing geraniol lipolysin) into the primary fermentation broth for further fermentation to obtain an improved tobacco stem extract. This invention creatively provides a method for improving tobacco stem extract using multi-microorganism synergistic fermentation. This method effectively degrades macromolecular components such as cellulose, hemicellulose, pectin, and starch in the tobacco stem extract while increasing the content of aroma components in the tobacco stem extract. This significantly reduces the off-flavors and irritation of the treated improved tobacco stem extract, improves its sensory quality, and significantly enhances its reclaiming value.

A method for improving the synthetic ability of *Yersinia lipolytica* to synthesize amino acid derivatives. This invention discloses an engineered strain for the biological synthesis of the amino acid derivative β-hydroxy-β-methylbutyric acid (β-methylbutyric acid) and its construction method. Using *Yersinia lipolytica* as the substrate cell, the HMGCL and OCT genes involved in branched-chain amino acid metabolism in its genome are knocked out to obtain a gene knockout strain. Decarboxylase genes, hydratase genes, dehydratase genes, and thioesterase genes are integrated into the genome of the gene knockout strain to obtain an engineered strain capable of de novo synthesis of β-hydroxy-β-methylbutyric acid. Further integration of the CcGCTB-CcGCTA fusion gene expression module enhances the expression level of key enzyme genes, thereby increasing the yield of β-hydroxy-β-methylbutyric acid in the strain. The engineered strain constructed in this invention can synthesize the target product β-hydroxy-β-methylbutyric acid using glucose as a carbon source through fermentation, with a maximum yield of up to 30 g / L.

This invention discloses an engineered strain of *Yersinia lipolytica* for synthesizing astaxanthin, its preparation method and application, a method for producing astaxanthin, and a method for increasing astaxanthin yield. The engineered strain of *Yersinia lipolytica* for synthesizing astaxanthin is obtained by expressing phytoene dehydrogenase CarB, phytoene synthase / lycopene cyclase CarRP, β-carotene ketolase CrtW, β-carotene hydroxylase CrtZ, and ferricoxane reductase RFNR in a host strain. RFNR Derived from Chlamydomonas rhinelandii Chlamydomonas reinhardtii Haematococcus pluvialis Haematococcus pluvialis This strain, through heterologous expression of RFNR, efficiently provides reducing power to CrtZ, significantly enhancing the catalytic activity of CrtZ and ultimately achieving higher astaxanthin yield.

This invention provides a composition, kit, and application for triple real-time fluorescence PCR detection of pathogenic Yersinia pestis based on TaqMan probes. Specific primers and TaqMan probes are designed targeting the caf1 gene of Yersinia pestis, the foxA gene of Yersinia enterocolitica, and the inv gene of Yersinia pseudotuberculosis, enabling simultaneous detection of these three pathogens in a single reaction system. The optimized annealing temperature is 54℃, and the final probe concentration is 0.3 μM. This method exhibits good linearity (R²>0.99), with detection limits of 5×10² copies / μL for I9 and C4, and 1×10¹ copies / μL for F4. Intra-assay and inter-assay coefficients of variation are mostly below 2%, and there is no cross-reactivity with non-target bacteria. It demonstrates good sensitivity, specificity, and repeatability, and can be used for rapid detection of pathogenic Yersinia pestis in food, environmental, clinical, and animal-derived samples.

This invention belongs to the field of bioengineering and discloses a *Yersinia lipolytica* chassis strain with homologous recombination preference, its construction method, and its applications. It involves inserting endogenous genes Rad52, Rad50, Mus81, and Mms4, as well as the single-stranded DNA annealing protein (PapRecT) encoded by *Pseudomonas aeruginosa* bacteriophage and its corresponding host *P. aeruginosa*-derived single-stranded DNA binding protein (PaSSB), into the genome of the *Yersinia lipolytica* strain, followed by knocking out its endogenous Rad9, resulting in a *Yersinia lipolytica* chassis strain with homologous recombination preference. This invention's *Yersinia lipolytica* chassis strain achieves effective improvement in homologous recombination efficiency through multi-dimensional regulation of the homologous recombination process, constructing an excellent *Yersinia lipolytica* platform, which is beneficial for further genome editing and has significant implications for using *Yersinia lipolytica* as a chassis cell in basic or applied research.

This application relates to the field of microbial engineering technology, specifically to a *Yersinia lipolytica* yeast, its application, and a method for producing conjugated linolenic acid (CLNA). This application provides a *Yersinia lipolytica* yeast, with accession number CGMCCNO.39103, and provides its application in producing CLNA. It also provides a method for producing CLNA, comprising the following steps: (1) inoculating a *Yersinia lipolytica* yeast strain into a culture medium rich in linolenic acid substrate, fermenting to obtain a *Yersinia lipolytica* yeast strain rich in CLNA; (2) extracting fatty acids using an organic solvent to obtain CLNA. The *Yersinia lipolytica* yeast strain of this application not only produces high levels of CLNA, but also synthesizes CLNA with conjugated double bonds located at carbon atoms 11, 13, and 15 at the carboxyl terminus, similar to natural CLNA; furthermore, the method for producing CLNA has the advantages of high efficiency and low cost.

We have shown that cprA expression leads to the production of outer membrane vesicles (OMVs) that block autophagic flux and have a greater capacity to activate the non-canonical inflammasome pathway. In a murine model of sepsis, a P. aeruginosa strain deleted for cprA was less virulent than the wild-type strain. These results demonstrate the important role of CprA in the pathogenicity of P. aeruginosa. It is worth noting that CprA is also a functional ortholog of HlyF, which is encoded by virulence plasmids of Escherichia coli. We have shown that other cryptic SDRs encoded by mammalian and plant pathogens, such as Yersinia pestis and Ralstonia solanacearum are functional orthologs of CprA and HlyF. These SDRs also induce the production of OMVs which block autophagic flux. This study uncovers a new family of virulence determinants in Gram-negative bacteria, offering potential for innovative therapeutic interventions and deeper insights into bacterial pathogenesis. The present invention relates to a method for producing vesicles, the method comprising a step of expressing or overexpressing hemolysin F (hlyF) gene or an ortholog of hemolysin F gene in Gram-negative bacteria, said hlyF gene or ortholog of hlyF gene encoding respectively a HlyF protein or an ortholog of HlyF protein.

This invention discloses a method and engineered strain for synthesizing multiple types of retinyl esters using *Yarrowia lipolytica*, relating to the field of biotechnology. The method for constructing the engineered strain includes the steps of introducing key genes into a *Yarrowia lipolytica* starter strain, overexpressing the key genes, and obtaining the engineered strain. The key genes include the ybbO gene, the LRAT gene, and the PaGGPPS gene. The nucleotide sequence of the ybbO gene is shown in SEQ ID NO.1; the nucleotide sequence of the LRAT gene is shown in SEQ ID NO.2; and the nucleotide sequence of the PaGGPPS gene is shown in SEQ ID NO.3. The engineered strain constructed by this invention can utilize multiple carbon sources to achieve de novo synthesis of retinyl esters. The product exhibits superior stability and safety compared to retinol, and comparable antioxidant and anti-aging activities, making it suitable for industrial production in cosmetics, food, and other fields.

This invention discloses a strain of *Yarrowia lipolytica* that produces crocin, classified and named as follows: Yarrowia lipolytica OUC-Cro13-EL8GGP, deposited at the China Center for Type Culture Collection (CCTCC) NO: M 20252999, date of deposit: December 24, 2025. This strain is used in the preparation of crocin. This invention successfully obtained a high-yielding *Yersinia lipolytica* genetically engineered strain capable of producing crocin-1, achieving a yield of 21.3 mg / L, through strategies such as heterologous gene transfer, overexpression of key enzymes, regulation of key metabolic nodes, and modular enzyme assembly and expression. This demonstrates the efficient synthesis of crocin in *Yersinia lipolytica*. The crocin-producing *Yersinia lipolytica* strain of this invention can be used for large-scale crocin production, showing potential for industrial-scale production and promising application prospects.

This invention provides a genome editing method for *Yersinia lipolytica* based on homologous recombination, belonging to the fields of microbial genetic engineering and synthetic biology. This invention constructs the tool plasmid pUrloxP3 to achieve efficient knockout and combined knockout of ylAS and ylPDH, completing site-specific integration and functional verification of exogenous genes RgTAL, 4CL, and CHS. The Cre / loxP system is used to recover the URA3 marker and eliminate the pYLXP'-Cre helper plasmid. This invention features high editing efficiency, standardized procedures, cyclical selection markers, and stable integration of exogenous genes, ultimately yielding a stable engineered strain without markers or helper plasmids. It is suitable for multi-round metabolic engineering and cell factory construction of *Yersinia lipolytica*.

This invention discloses a method for constructing a highly efficient mannitol-synthesizing *Yersinia lipolytica* strain and a proprietary strain. Using *Yersinia lipolytica* as the substrate microorganism, this invention modifies the yeast through systemic metabolic engineering, significantly reducing the synthesis of byproducts and greatly enhancing the yield, conversion rate, and production efficiency of the target product, mannitol. The CGMCC No. 39179 strain constructed in this invention can efficiently and rapidly synthesize mannitol from glucose. In a 200-liter fermenter, this strain achieved a mannitol yield of 254.4 g / L, a conversion rate of 58.1%, and a synthesis efficiency of 3.18 g / L / hour, representing the highest reported yield of mannitol synthesized by microorganisms using glucose as a carbon source.