104 results about "Yersinia" patented technology

The present invention provides isolated polypeptides isolatable from a Yersinia spp. Also provided by the present invention are compositions that include one or more of the polypeptides, and methods for making and methods for using the polypeptides.

The present disclosure relates to compositions, methods, systems and kits for the detection of microorganisms of the Yersinia species including Yersinia pestis. The disclosure relates to recombinant phage operable to infect a Yersinia microorganism, the phage comprising a detectable reporter. Detection systems of the disclosure may comprise a phage operable to infect a Yersinia microorganism, and may comprise a reporter nucleic acid expressible upon infection of a Yersinia microorganism by the phage. The system may be operable to detect the expression of the reporter. A detectable reporter may comprise any gene having bioluminescent, colorimetric and/or visual detectability. For example, a detectable reporter may comprise one or more luxAB genes detectable by emission, enhancement and/or change in spectrum of bioluminescent light. Live and infectious Yersinia microbes may be detected by the compositions, methods, systems and kits described herein.

The present invention provides a gene chip for quickly detecting pathogens from food source, discloses the preparation method of said gene chip and provides 26 oligonucleotide probe sequences for detection. Said gene chip can quickly, accurately and high-effectively detect and identify the class of the pathogens in food, and its detection range includes staphylococcus aureus, Shiga's bacillus, salmonella, colibacillus 0157, bacillus proteus, mononuclear hyperplastic listerella, enterocolitis yersinia, aeruginous pseudomonads, vibrio parahaemolyticus, vibrio cholerae, bacillus cereus, beta hemolytic streptococcus, coconut fermentation pseudomonads, boticin, vibrio jejuni and bacillus perfringens, etc.

The present invention relates to a safe non-virulent Yersinia enterocolitica mutant strain for delivering heterologous proteins in target cells carrying mutations in at least one of the effector genes yopH, yopO, yopP, yopE, yopM, yopT genes and at least one additional mutation in the invasin genes chosen from yadA and/or inv. The present invention also relates to a safe non-virulent Yersinia enterocolitica mutant strain for delivering heterologous proteins in target cells according to claim 1 carrying mutations in all effector genes yopH, yopO, yopP, yopE, yopM, yopT genes and at least one additional mutation in the invasin genes chosen from yadA and/or inv. The present invention also relates to an expression vector for delivering a heterologous protein into a target cell using a Yersinia enterocolitica mutant strain according to any of the claims 1 to 4, which comprises in the 5' to 3' direction :(a) a promoter of a Yersinia virulon gene, (b) a first DNA sequence encoding a delivery signal from a Yersinia effector protein, operably linked to said promoter; and, (c) a second DNA sequence coding for said heterologous protein, fused in frame to the 3' end of said first DNA sequence. The present invention further relates to methods and compositions comprising (the use of) the afore-mentioned mutant strains and expression vectors.

The invention relates to a bovine brucella indirect ELISA antibody detection kit. The kit uses the lipopolysaccharide (LPS) of brucella suis S2 acclimated and induced by the application institution in the 1960s as the coating antigen, and improves the sensitivity of detection. The study improves the LPS purification and purity determination method, and endows the developed kit with good specificity. The kit not only can effectively eliminate the interference of conventional Gram negative bacteria to Brucella, but also can get rid of the technical defect that general brucellosis indirect ELISA (enzyme-linked immunosorbent assay) kits cannot distinguish enterocolitis Yersinia O9 and Escherichia coli O157 interference, and improves the specificity of detection. Through improvement and optimization of the main reagent formula, the reaction background of the kit is effectively controlled, and also the time required by detection is shortened.

The invention relates to a multiplex PCR-based synchronous and rapid method for detecting 13 pathogenic microorganisms in water, which comprises the steps of using multiplex PCR to simultaneously amplify gene-specific fragments of the 13 pathogenic microorganisms including escherichia coli, enterohaemorragic escherichia coli o157:h7, legionella pneumophila, salmonella enteritidis, shigella dysenteriae, staphyloccocus aureus, listeria monoeytogenes, helicobacter pylori, mycobacterium tuberculosis, klebsiella pneumonia, vibrio cholera, bacillus anthracis and yersinia pestis, and detecting PCR amplified products through agarose gel electrophoresis, thereby achieving synchronous and rapid detection for the 13 pathogenic microorganisms.

Provided are methods for growing and shipping sprouts and microgreens in the same container, growing while in shipment using moisture provided in a water-absorbent layer, with optional added beneficials, and including methods for producing sprouts and microgreens for consumption, and for pharmaceutical/nutriceutical use, comprising growth of sprouts in retail-ready containers, the container comprising a moisture-retaining layer of agar media or the like providing water for growth and obviating the need for irrigation during sprout growth. In certain aspects, media is supplemented with beneficial organisms or additives such as probiotic microbes, vitamins (e.g., B12), cofactors, nutrients, and other items (e.g., phytochemicals, natural colors, and antioxidants) which promote the growth of the beneficial microbes on the product, and/or which become incorporated into the product. In certain aspects, added beneficial microorganisms are selected to compete antagonize human pathogens such as Listeria, Salmonella, enterohaemorrhagic E. coli, Yersinia, and/or spoilage organisms (e.g., Erwinia, Pseudomonas and Xanthomonas).

The disclosure relates to the targeting of Y. pestis mediated by the binding activity of tail fibers from naturally occurring R-type pyocins from Pseudomonas aeruginosa. The targeting may be mediated by a macromolecular complex such as the pyocin itself, a high molecular weight (hmw) bacteriocin modified to have the tail fiber's binding activity, or a bacteriophage modified to have the tail fiber's binding activity. Compositions comprising such complexes are described. Also disclosed are methods for the use of a complex, such as to inhibit the growth of a Yersinia species like Y. pestis, by compromising the integrity of its cytoplasmic membrane are also described. Additional methods include use of the binding activity to identify Y. pestis.

The invention discloses a method for detecting food origin enterocolitis Yersinia by using loop-mediated constant temperature PCR technology, belonging to the food sanitation field. The technical proposal is as follows: enterocolitis Yersinia 16S-23S regions are used as target detection sequences, and four specific primers containing the whole region sequences are designed. The invention comprises extraction of four specific primers of enterocolitis Yersinia 16S-23S regions and food origin enterocolitis Yersinia, detection method for loop-mediated constant temperature PCR amplification and interpretation of results. The method fills the gap of a method for detecting food origin enterocolitis Yersinia by using loop-mediated constant temperature PCR amplification technology, and is used for checking enterocolitis Yersinia in import and export foods. The method has the characteristics of low cost, high efficiency, simple operation and convenience.

The invention discloses a gene chip-based method for synchronously and rapidly detecting thirteen pathogenic microorganisms in a water body. The method is characterized in that specific gene segments of the pathogenic microorganisms are amplified simultaneously by adopting multiple polymerase chain reactions (PCR), and then, a detection result is obtained through gene chip hybridization and chip scanning image analysis. The detected thirteen pathogenic microorganisms comprise escherichia coli, Enterohemorrhagic Escherichia coli O157:H7, legionella pneumophila, salmonella enteritidis, Shigella, staphylococcus aureus, Listeria monocytogenes, Helicobacter Pylori, mycobacterium tuberculosis, Klebsiella pneumonia, vibrio cholera, Bacillus anthracis and Yersinia pestis.

Techniques from two basic approaches, structure-based immunogen design and phage T4 nanoparticle delivery, are developed to construct new plague vaccines. The NH₂-terminal β-strand of F1 of Yersinia pestis is transplanted to the COOH-terminus of F1 of Yersinia pestis and the NH₂-terminus sequence flanking the β-strand of F1 of Yersinia pestis is duplicated to eliminate polymerization but to retain the T cell epitopes. The mutated F1 is fused to the V antigen of Yersinia pestis to thereby form a fusion protein F1mut-V mutant, which produces a completely soluble monomer. The fusion protein F1mut-V is then arrayed on phage T4 nanoparticles via a small outer capsid protein, Soc, from a T4 phage or a T4-related phage. Both the soluble and T4 decorated F1mut-V provided approximately 100% protection to mice and rats against pneumonic plague evoked by high doses of Yersinia pestis CO92.

The invention discloses a psychrotrophic bacteria strain Yersinia sp. KM1, a psychrotrophic alkaline lipase that is generated by the strain and resistant to organic solvents, and a separating and purifying method of the psychrotrophic alkaline lipase. The purifying method comprises the steps of ammonium sulfate precipitation of a fermented supernatant, centrifugal concentration with a 10KDa ultrafiltration pipe, Sephacry HRS-100 chromatography and Superdex G-75 chromatography, and finally obtains pure psychrotrophic alkaline lipase under electrophoresis. The purified psychrotrophic alkaline lipase has low reaction temperature, wider pH accommodation range and high resistance to organic solvents, and consequently has huge application potential in the fields of cleaning solvents, food processing, medicine production and biodiesel production, etc.

The invention relates to a novel isothermal loop-mediated yersinia pestis nucleic acid mark detection reagent. A front inner primer in a primer group for isothermal loop-mediated amplification is used for marking an antigen, and meanwhile, a rear inner primer in the primer group is used for marking another antigen, so that a yersinia pestis specific target gene can be simultaneously amplified and marked. A matched colloidal gold strip can be used for rapidly detecting an amplification product of the target gene after marking, thereby detecting yersinia pestis. The detection reagent can be simply and rapidly operated, and is high in specificity and sensitivity.

The invention discloses a yersinia pestis detection kit based on a real-time fluorescence RPA technology and application of the yersinia pestis detection kit. The kit package is composed of a primer pair and a probe with the name of YPS723-1-P1, wherein the primer pair is composed of single-stranded DNA with the name of YPS723-F1 and single-stranded DNA with the name of YPS723-R1. The kit disclosed by the invention can be used for rapidly and conveniently detecting yersinia pestis with high sensitivity and good specificity, and is expected to become a rapid diagnosis auxiliary tool for clinical samples.