Coloring culture medium for detecting food-borne pathogenic Yersinia

Abstract

The present invention discloses a coloring culture medium for detecting food-borne pathogenic Yersinia, wherein the culture medium contains soybean peptone, yeast powder, sodium chloride, sodium oxalate, agar, a beta-riboside chromogenic substrate, a beta-galactoside chromogenic substrate, esculin, ammonium ferric citrate, bile salt No.3, cephalosporin, novobiocin, and the balance of water. According to the present invention, the coloring culture medium has characteristics of simple formula and convenient preparation operation, can be used for the separation and the detection of food-borne pathogenic Yersinia, and further has advantages of specificity, high efficiency, rapidness, low cost, simple result determination, wide application prospect, and the like.

Description

technical field [0001] The invention relates to a culture medium for microorganism detection, in particular to a chromogenic culture medium for detecting food-borne pathogenic Yersinia, belonging to the field of food safety microorganism detection and monitoring. Background technique [0002] Yersinia enterocolitica is one of the important food-borne pathogenic bacteria, which can survive and proliferate in low-temperature preserved food, and can cause extremely serious food poisoning incidents. In some countries, the degree even exceeds Shigella, close to Salmonella and Campylobacter jejuni. Once infected, it will cause a variety of diseases and seriously threaten people's health. It is a routine inspection item for food safety in various countries. my country's food safety standard GB4789 .8 specifies the inspection method for Yersinia enterocolitica in food. [0003] Yersinia enterocolitica belongs to the genus Yersinia in taxonomy, and there are 11 species in this genus. ...

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Problems solved by technology

[0004] For the inspection method of Yersinia enterocolitica in food, the traditional method is commonly used at present. Taking the method of food safety standard GB4789.8 as an example, the specific operation is as follows: after the pre-enrichment and the treatment of the enrichment solution, inoculate CIN-1 and improved Y medium were used for culture and separation, and then the suspicious bacteria were tested for Klebsiella iron, urease, semi-solid dynamic test and microscopic examination to obtain the results of whether they belong to the genus Yersinia. It takes at least 6-7 days for biochemical reactions to distinguish Yersinia enterocolitica, and the efficiency is low

In addition, the detection of Yersinia enterocolitica and other Yersinia of this genus also uses PCR technology and other methods, which require special equipment and professional technicians, and the cost is high, which is not conducive to popularization and application. Currently, there is no food source Specific method for the sexual pathogen Yersinia pseudotuberculosis

[0005] The bacterial biochemical identification technology based on the traditional culture medium by adding specific enzyme substrates can be better connected with the traditional method at present, and the cultivation, separation and identification can be completed at one time, the operation is simple, the detection time is greatly shortened, and through The characteristic identification of target bacteria improves the accuracy, so it has broad application prospects. It has been applied to bacteria such as Salmonella, Vibrio, Listeria, and pathogenic E. coli O157 in many fields such as clinical medicine, food hygiene, and environmental protection. For the detection of Yersinia enterocolitica, CHROMagar has developed a chromogenic medium (CHROMagarY. Yersinia is the target, that is, Yersinia enterocolitica is divided into 6 groups according to phenotypic characteristics, and the chromogenic medium developed by CHROMagar can detect the remaining 5 groups with strong pathogenicity except 1A Enterocolitica Yersinia (groups 1B, 2, 3, 4, 5), that is, the target bacteria that can be isolated and identified by this medium are not all Yersinia enterocolitica, and cannot be isolated and detection of Yersinia pseudotuberculosis

The purpose of food safety inspection is to reduce the risk. As far as Yersinia enterocolitica is concerned, taking the method of food safety standard GB4789.8 as an example, the clear scope of inspection is all biological groups. Therefore, this product cannot meet the requirements of food The demand for safety testing, in addition, in the application of medical testing, its expensive price increases the cost of the user department

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