40 results about "Chromogenic Substrates" patented technology

The present application relates to the technical field of biotechnology, and particularly relates to a D-dimer chemiluminescence assay kit. The present application provides a D-dimer chemiluminescence assay kit, which comprises a magnetic particle suspension (immunomagnetic beads) coated with D-dimer antibody 1, alkaline phosphatase-labeled D-dimer antibody 2 (enzyme-labeled antibody) and a calibrant. The immunomagnetic beads and the enzyme-labeled antibody are prepared by chemical coupling and stored in a buffer containing a soluble high molecular material, which can effectively improve the detection sensitivity and repeatability. The kit can be used in combination with a substrate solution containing a chromogenic substrate such as adamantane and its derivative AMPPD or APS-5 for a full-automatic immune test system, and the content of D-dimer in plasma or whole blood can be determined by a chemiluminescence instrument. The kit has high detection sensitivity and good repeatability.

The application provides a method for constructing a double-signal sensing platform based on a non-copper nanometer enzyme AuPt@PBA@L-Pd and application, and a multifunctional nanometer composite material AuPt@L-Pd@PBA (hereinafter referred to as APL) with the effects of an oxidase-like enzyme, a peroxidase-like enzyme and a laccase-like enzyme is prepared. The composite nanometer enzyme has a regular cubic morphology, takes PBA as a base material, loads L-Pd through an amide bond, simultaneously collects the advantages of the MOF material PBA and L-Pd, has multiple enzyme-like effects, and can catalyze different chromogenic substrates. The AuNPs and PtNPs on the surface of APL not only provide good binding sites for antibodies, but also successfully prepare a biological composite probe Ab2@APL, further amplify the sensing signal, and improve the sensitivity of the sensing platform. It is worth mentioning that APL has the effect of a laccase-like enzyme, but does not contain copper ions, and further improves the biocompatibility. Based on the high catalytic activity and multifunctional nanometer enzyme Ab2@APL, a multi-signal sensing platform can be constructed, and the application prospect in more fields is wider.

This invention relates to the field of tetracycline detection technology, specifically disclosing an iron porphyrin hydrogen-bonded organic framework nanozyme, its preparation method, and its applications. The iron porphyrin hydrogen-bonded organic framework nanozyme comprises an iron porphyrin hydrogen-bonded organic framework, gold nanoparticles, and a tetracycline nucleic acid aptamer. The gold nanoparticles are non-covalently loaded onto the surface and pores of the iron porphyrin hydrogen-bonded organic framework, and the tetracycline nucleic acid aptamer and gold nanoparticles are connected by coordination covalent bonds. The iron porphyrin hydrogen-bonded organic framework nanozyme exhibits peroxide-like nanozyme activity, catalyzing the oxidation of the chromogenic substrate TMB in the presence of H₂O₂ to detect tetracycline content. By introducing the hydrogen-bonded organic framework and the tetracycline nucleic acid aptamer, the detection specificity is improved, achieving highly sensitive, highly selective, and rapid detection of tetracycline. The colorimetric sensor constructed based on the iron porphyrin hydrogen-bonded organic framework nanozyme provides a new method for rapid and visual detection of tetracycline in feed.

The utility model provides a kind of equipment for dry chemical chromogenic substrate method detection, it is related to dry chemical analysis inspection equipment technical field.The equipment for dry chemical chromogenic substrate method detection, including base, top isolation component and bottom isolation component, is equipped with test hole on base, top isolation component includes transparent observation board, the top of base is equipped with the first through slot being communicated with test hole, transparent observation board is set in the first through slot, and can be slid into test hole along the first through slot extension direction, and test hole is blocked.Bottom isolation component includes upper plate body and lower plate body, the bottom of base is equipped with the second through slot for sending test paper, second through slot is communicated with test hole, lower plate body is coincident with upper plate body and forms conveying body, test paper groove for placing test paper is equipped on the lower plate body of upper plate body side, conveying body is set in second through slot.The utility model can avoid test paper exposure in external environment, greatly improve the accuracy of detection result.

The application belongs to the technical field of sensors and relates to an array sensor based on cerium-based metal organic framework nanoscale enzyme and detection application of perfluoroalkyl compounds. The array sensor is a Ce-MOF triple enzyme activity array sensor and has multiple independent sensing channels. The sensing channels contain Ce-MOF nanoscale enzyme and chromogenic substrate. The chromogenic substrate includes first, second and third chromogenic substrates. The first chromogenic substrate includes 3,3',5,5'-tetramethylbenzidine. The second chromogenic substrate includes 2,4-diamino phenol and 4-aminoantipyrine. The third chromogenic substrate includes xanthine, xanthine oxidase and nitrogen blue tetrazolium. The MOF nanoscale enzyme array sensor constructed by the application has high sensitivity and selectivity in the identification and differentiation of PFASs compounds. The array sensor can not only accurately distinguish single PFASs compounds but also quantitatively detect the compounds, and has good response to PFASs with different concentrations.

The invention discloses a wearable sensor-minimally invasive diabetes monitoring patch and a preparation method thereof, and belongs to the technical field of biomedicine, the patch comprises a microneedle array module and a sensing unit; the microneedle array module is composed of a plurality of microneedles arranged in an array; the sensing unit is loaded in the microneedle, and the sensing unit comprises a multi-shell metal organic framework cascade enzyme system and a chromogenic substrate; the multi-shell metal organic framework cascade enzyme system is of a double-layer structure composed of an inner core and an outer shell, the inner core is a metal organic framework packaged with glucose oxidase and modified with carboxyl functional groups, and the outer shell is a metal organic framework packaged with horse radish peroxidase. And 2, 2-nitrogen-bis (3-ethylbenzothiazoline-6-sulfonic acid) is taken as a chromogenic substrate. According to the patch, the catalytic efficiency and the detection accuracy are improved, the stability is improved, the storage and transportation cost is reduced, the low detection limit can be achieved, the requirement for instant blood glucose detection is met, the size is small, and the cost is low.

The invention discloses a preparation method of cerium-based nano enzyme, which comprises the following steps: reacting cerium salt and zinc salt with 2-methylimidazole in methanol, then loading into a high-pressure reaction kettle to prepare a Ce-MOF precursor, then calcining the precursor in a tubular furnace to obtain powder, and finally pickling the powder to obtain the cerium-based nano enzyme. The prepared cerium-based nano-enzyme is used for uric acid detection, can replace natural enzyme for quantitative analysis, and has the advantages of high sensitivity, low detection limit and better selectivity and stability. The invention also provides a quantitative analysis method for detecting uric acid, which comprises the following steps: reacting uric acid with uricase to generate hydrogen peroxide, then catalyzing the hydrogen peroxide by the cerium-based nano-enzyme to oxidize a chromogenic substrate, and finally quantifying according to a color B value, absorbance or temperature.

The invention relates to the technical field of early risk assessment of gynecological tumors, and discloses a tumor risk assessment system based on an epithelial ion channel protein expression nanoprobe. Comprising a detection composition which is prepared into a secondary antibody containing a chromophoric enzyme label or a fluorescent substance label, a chromogenic substrate and a buffer solution; the diagnosis kit is configured to contain the detection composition and provide an integrated micro-fluidic chip for bearing a sample; the computer equipment is configured to obtain channel activity change data monitored by the micro-fluidic chip in real time through a multi-time impedance method, calculate a real-time sampling error based on the multiple groups of channel activity data, adjust sampling parameters to enable the sampling error to reach a continuous minimum variance, and optimize the color development signal or the development signal; and carrying out fusion analysis on the optimized protein expression data, imaging evidence and physical and chemical inspection data to generate a gynecological tumor risk collaborative estimation result.

This invention provides a colorimetric / fluorescence dual-mode nanozyme aptamer sensor, its preparation method, and its application in bisphenol A (BPA) detection. The sensor comprises a metal-organic framework nanozyme loaded with a BPA aptamer, hydrogen peroxide, and a chromogenic substrate, TMB. Cu / Zr-MOF oxidizes the colorless TMB to blue oxTMB, producing a UV absorption peak at 652 nm and a fluorescence emission peak at 415 nm. Modification with the BPA aptamer enhances the specific recognition ability of BPA, while simultaneously inhibiting the enzyme activity of Cu / Zr-MOF, leading to weakened colorimetric and fluorescence signals. In the presence of BPA, the aptamer specifically binds to the target and dissociates from the nanozyme surface, restoring enzyme activity and enhancing colorimetric and fluorescence signals. This invention integrates the excellent catalytic ability of nanozymes with the specific recognition ability of aptamers into a single system, achieving highly sensitive BPA detection.

The invention relates to the technical field of detection, and relates to a single-molecule protein detection method based on a microarray chip and a chemiluminescence immunoassay technology. An antigen-antibody complex reaction solution is randomly segmented into tens of thousands of microarray holes with the single-hole volume at the pL level by utilizing a microarray chip containing 0.5-200 thousands of micropores with the diameter of 5-30 [mu] m. Each micropore is used as an independent reaction unit for detection and comprises one or zero antigen-antibody compounds, a chromogenic substrate or exciting light is added to collect signal data in all the micropores, and the number of all the antigen-antibody compounds in a reaction system is directly and quantitatively detected according to a Poisson distribution calculation formula. And calculating the concentration of the antigen captured in the microarray chip. The method disclosed by the invention has the advantages of small background signal interference, high sensitivity, specificity, accuracy and precision, simplicity and rapidness in operation, low cost and obvious advantages in the technical field of single molecule detection, and the lower limit of detection reaches fg / ml.

A disposable bioassay diagnostic cartridge for performing enzyme-linked immunosorbent assay for a single patient in whole blood and plasma where the cartridge has a plurality of wells. The cartridge includes a pipette tip with internal sidewalls disposed in one well of the plurality of wells, the internal sidewalls being pretreated and coated with antibodies, a reagent mixture disposed in another well of the plurality of wells, the reagent mixture comprising at least one reagent selected from the group consisting of pH stabilizer, ionic catalyst control agent, ionic intracellular inhibitory control agent, detergent, negative control agent, chelating agent, and biocide preservative, antibody, and conjugate stabilizing agent. At least a chromogenic substrate and visualizing reagent disposed in an integrated cuvette of the plurality of wells. It also includes the detection of the hemoglobin amount in the blood sample so a correction for plasma amount in a sample can be performed.

The invention discloses a colorimetric sensing array based on hierarchical pore nano-enzyme as well as a construction method and application of the colorimetric sensing array, and belongs to the technical field of analysis and detection. According to the array, two cerium-based MOF nano-enzymes, namely micropore UiO-66 and hierarchical pore HP-UiO-66, are respectively combined with TMB (tetramethylbenzidine) and ABTS (2, 2, 6, 6-tetramethylbenzidine) chromogenic substrates to form a four-channel sensing system. The method comprises the following steps: synthesizing hierarchical pore HP-UiO-66 by a template method; collecting the absorbance of each channel under the characteristic wavelength by using an array, and generating a specific fingerprint spectrum of the to-be-detected object; by combining mode recognition technologies such as linear discriminant analysis and the like, rapid and synchronous distinguishing and quantitative detection of at least eight antioxidants with similar structures such as glycyrrhizic acid and caffeic acid in the traditional Chinese medicine decoction are realized. According to the method, the catalysis and mass transfer efficiency is improved by regulating and controlling the pore structure of the nano-enzyme, the problem of multi-target synchronous recognition in a complex matrix is solved by utilizing array cross response, and the method has the advantages of simplicity and convenience in operation, high flux and good accuracy.

The invention belongs to the technical field of biomedical detection and immunoassay, and particularly discloses a mycobacterium tuberculosis detection probe as well as a preparation method and application thereof. The mycobacterium tuberculosis detection probe provided by the invention comprises a capture probe and a signal probe, the capture probe is magnetic nanoparticles of which the surfaces are fixedly loaded with a mycobacterium tuberculosis specific antibody 1, and the signal probe is a mycobacterium tuberculosis specific antibody 2 of which the surface is marked with a photosensitizer. According to the mycobacterium tuberculosis detection probe disclosed by the invention, a photosensitizer labeling technology is combined with immunoassay, so that high-sensitivity detection of mycobacterium tuberculosis can be realized under a mild condition; under the irradiation of visible light, active oxygen generated by the photosensitizer can catalyze oxidation reaction of various chromogenic substrates, so that rapid colorimetric detection of mycobacterium tuberculosis is realized, and the kit is particularly suitable for the requirements of clinical serum sample detection and the like; in addition, the fluorescent probe also has the advantages of simple preparation process, strong recognition specificity, high detection sensitivity, good stability and reproducibility and the like.

The invention provides an asphalt carbon-based oxidase-like nano material and a preparation method and application thereof.In the preparation method of the asphalt carbon-based oxidase-like nano material, starting from modification of a coal liquefaction asphalt base material, the asphalt carbon-based oxidase-like nano material is prepared through a series of links of coal liquefaction asphalt activation, metal ion loading, nitrogen doping, heat treatment and the like. And the asphalt carbon-based oxidase-like nano material with high specific surface area, rich porous structure, stable metal active sites and optimized electron distribution is gradually constructed. Through the elaborately designed steps, the material can efficiently catalyze oxidation of a chromogenic substrate without participation of hydrogen peroxide, visual detection of the antioxidant is achieved, and meanwhile it is ensured that the detection method has the remarkable advantages of being low in cost, easy and convenient to operate, high in sensitivity and wide in applicability; and powerful support is provided for technical innovation in the fields of food safety and health monitoring.

The invention provides a FeCoP-NC diatomic nano enzyme as well as a preparation method and application thereof. The used FeCoP-NC nano-enzyme has active sites of FeN3P-CoN3P, the mass fraction of doped P is 0.08%-0.12%, the mass fraction of Fe is 0.25%-0.32%, the mass fraction of Co is 0.5%-0.58%, the FeCoP-NC nano-enzyme is of a dodecahedron structure, the surface area of the FeCoP-NC nano-enzyme is 810-830 m < 2 > / g, the FeCoP-NC nano-enzyme is of a porous structure, and the pore diameter of the FeCoP-NC nano-enzyme is 3-3.5 nm. The FeCoP-NC nano-enzyme disclosed by the invention has excellent oxide-like enzyme activity, and can be used as a catalyst for catalyzing a chromogenic reaction of a chromogenic substrate in a colorimetric sensor.

The invention discloses a recombinant tachypleus amebocyte lyophilized preparation for bacterial endotoxin detection as well as a preparation method and application of the recombinant tachypleus amebocyte lyophilized preparation. The recombinant tachypleus amebocyte lyophilized preparation is obtained by lyophilizing a lyophilized solution, wherein the lyophilized solution comprises the following components: mannitol, cane sugar, polyvinylpyrrolidone, recombinant human albumin, ascorbic acid, sodium chloride, inositol, L-arginine hydrochloride, ethylene glycol diethyl ether diamine tetraacetic acid, Tween 80, Proclin 300, Tris-HCl with the pH value of 7.0-8.0, a recombinant tachypleus amebocyte lyophilized preparation, a chromogenic substrate and a reaction buffer solution. The invention further discloses a preparation method and application of the recombinant tachypleus amebocyte lyophilized preparation. The recombinant tachypleus amebocyte lyophilized preparation is prepared by matching a multi-element synergistic protection system with a seven-stage gradient temperature control freeze-drying process, and can be used for high-sensitivity and high-stability detection of bacterial endotoxin in drugs, medical instruments, biological products and clinical samples.

The application relates to the technical field of biological medicine, and particularly discloses a kit for determining antithrombin III activity, which comprises a first reagent, a second reagent, a third reagent and a diluent; the first reagent at least comprises a first buffer, thrombin, a serine protease inhibitor and a first stabilizer; the second reagent at least comprises a second buffer, a chromogenic substrate and a second stabilizer; the third reagent at least comprises a third buffer, heparin, isopropyl alcohol and a third stabilizer; and the diluent at least comprises physiological saline and a fourth stabilizer. In the kit, the cost is low, the anti-interference capability is strong, the sensitivity is high, the stability is good, and the kit has a wide application prospect.

The application discloses a rivaroxaban concentration determination method based on anti-Xa factor activity, a determination reagent and a preparation method thereof, and relates to the technical field of biology, and comprises reagent R1, reagent R2 and a sample diluent.The reagent R1 comprises Xa factor, a buffer, a stabilizer and inorganic salt.The reagent R2 comprises a chromogenic substrate, a buffer, polyethylene glycol and inorganic salt.The sample diluent comprises a buffer, a stabilizer and inorganic salt and is used for diluting samples with a concentration higher than a preset threshold value.The application remarkably reduces the cost by adopting an optimized formula of bovine factor and domestic raw materials, ensures the detection accuracy of high-concentration samples, and has excellent sensitivity, specificity and stability, realizes a detection performance highly related to a gold standard method, and thus successfully overcomes the technical bottlenecks of high detection cost, limited linear range, inaccurate detection of high-value samples and lack of high-quality domestic reagents in the prior art.

The invention discloses a trimetal nano-catalyst, preparation thereof and application of the trimetal nano-catalyst in detecting and degrading antibiotic drugs. The preparation method specifically comprises the following steps: mixing molybdenum disulfide powder and polyvinylpyrrolidone, dispersing the mixture in a mixed solution of ethanol and water to obtain a suspension, and performing post-treatment to obtain a two-dimensional molybdenum disulfide nanosheet solution; and mixing the two-dimensional molybdenum disulfide nanosheet solution with a chloroauric acid solution and a chloroplatinic acid solution, heating in a water bath for reaction, cooling and centrifuging to obtain the trimetal nano-catalyst. The preparation method of the catalyst is green and mild, a reducing agent does not need to be added, deposition of precious metal can be completed in a low-temperature water phase through the reducibility of molybdenum disulfide, and the catalyst can catalyze hydrogen peroxide to generate hydroxyl free radicals to efficiently degrade antibiotics such as tetracycline and the like; the rapid and high-sensitivity colorimetric detection of the antibiotics can be realized based on the competitive oxidation reaction of the antibiotics and the chromogenic substrate, and the method can be used for monitoring and treating antibiotic drugs.

The present invention relates to a real-time bacteria detection and identification device (1) comprising a body, said body comprising: an inlet opening (2) and an outlet opening (3), configured for passage of a fluid; a central portion (4) located between the inlet opening (2) and the outlet opening (3), said central portion (4) being configured to allow the visualization of an at least one porous polymer-based matrix (5), said matrix (5) comprising at least one polymer, said polymer comprising at least one entrapped lysis compound, at least one immobilized bacteria attracting compound and at least one immobilized chromogenic substrate, wherein said immobilized chromogenic substrate of the polymer-based matrix (5) is configured to change its colour when contacted with a bacterium containing fluid.

The application discloses a chromogenic differential culture medium for separating duck Riemerella anatipestifer and application thereof, and belongs to the field of microorganism detection. The application provides the chromogenic differential culture medium based on physiological and biochemical characteristics of the duck Riemerella anatipestifer, and the chromogenic differential culture medium takes polymyxin B and vancomycin as specific antibiotics and takes X-Glu as a chromogenic substrate. The experimental results show that the chromogenic differential culture medium can efficiently inhibit growth of miscellaneous bacteria, and the duck Riemerella anatipestifer colony presents a characteristic color, so that the dual functions of selective culture and rapid identification are realized; the duck Riemerella anatipestifer identification method developed based on the chromogenic differential culture medium has the advantages of good selectivity, intuitive identification, simple operation, accurate results and the like, and the application provides new materials and methods for identification of the duck Riemerella anatipestifer and diagnosis of duck Riemerella anatipestifer disease.

The invention belongs to the technical field of biomedical detection and immunoassay, and particularly discloses a parathyroid hormone detection probe as well as a preparation method and application thereof. The parathyroid hormone detection probe provided by the invention comprises a capture probe and a signal probe, the capture probe is a magnetic particle of which the surface is fixed with a PTH specific antibody 1, and the signal probe is a PTH specific antibody 2 of which the surface is marked with a photosensitizer. The immunodetection probe provided by the invention can solve the problems of unstable enzyme activity, high detection cost, complex operation and the like in the existing enzyme labeling immunodetection technology. Different from the traditional chemiluminescence or enzyme catalysis signal amplification mechanism, the photosensitization oxidation reaction and the double-antibody sandwich immune recognition are creatively combined, and the color change of the chromogenic substrate is catalyzed by utilizing the characteristic that the photosensitizer efficiently generates active oxygen under the irradiation of visible light, so that the high-sensitivity and high-specificity detection on the to-be-detected object PTH is realized.

The application belongs to the technical field of hematin quantitative detection, and specifically discloses application of methylene blue cyclopropyl formylated derivative as a chromogenic substrate for detecting hematin, and uses methylene blue cyclopropyl formylated derivative AMB as a chromogenic substrate for detecting hematin Hemin; compared with a classical substrate TMB, the methylene blue cyclopropyl formylated derivative AMB of the application has higher sensitivity in the detection of hematin Hemin; in addition, the amino protons of the methylene blue cyclopropyl formylated derivative AMB of the application are fully substituted by methyl, which inhibits the participation of active oxygen free radicals in oxidation, but is still sensitive to the oxidation pathway of ferric free radicals, has better selectivity compared with TMB, and the AMB system can effectively detect hematin Hemin in a serum sample. Therefore, it can be said that for the detection of hematin Hemin, AMB is a more excellent colorimetric substrate than TMB.

This invention provides an electrochemical sensor for detecting PDGF-BB using a poly A-DNA tetrahedral probe and its application. The electrochemical sensor is a base-stack-dependent electrochemical sensor, comprising a poly A-tetrahedral probe self-assembled on a gold electrode, aptamer DNA, horseradish peroxidase, and a chromogenic substrate. The aptamer DNA is biotin-modified at its tail, and the horseradish peroxidase is avidin-modified. The electrochemical sensor detects PDGF-BB. The aptamer DNA is triggered by PDGF-BB to transform into a stem-loop structure and stacks with the poly A-tetrahedral probe. Based on the base stacking effect, the aptamer DNA stably hybridizes with the target complementary sequence of the poly A-tetrahedral probe immobilized on the gold electrode. The aptamer DNA and horseradish peroxidase bind via biotin-avidin. The chromogenic substrate is catalyzed to generate an electrochemical redox catalytic signal, which is then output.

The invention provides a kit for detecting endotoxin and a detection method thereof. The kit comprises: a capture unit: a recombinant crab-like hemocyanin factor C fixed on the surface of a solid phase carrier; the signal probe is composed of a polyclonal anti-lipopolysaccharide antibody coupled with gold nanoparticles and horse radish peroxidase; the reaction buffer solution is a Tris-HCl buffer solution containing calcium ions required by activation of Factor C and a surfactant; the signal substrate solution is a peroxidase chromogenic substrate solution containing 3, 3 ', 5, 5'-tetramethyl benzidine; the stop solution comprises a dilute sulphuric acid solution and an endotoxin standard substance. The rapid, high-sensitivity and high-specificity endotoxin detection kit based on the recombinant factor C and the nano signal amplification technology has the advantages of high specificity, interference resistance, high sensitivity, rapidness, convenience and the like, and is wide in application prospect.

The application relates to a tannic acid detection method based on Prussian blue nanoscale enzyme and application thereof, and the method comprises the following steps: adding HCl in K3[Fe(CN)6] * 3H2O, heating reaction, centrifugal collection of the precipitate, washing and drying to obtain Prussian blue nanoscale enzyme; mixing the aqueous solution of the Prussian blue nanoscale enzyme, a chromogenic substrate, peroxide and tannic acid standard working solutions with different concentrations, water bath reaction, measuring the absorbance at 650-655 nm, and drawing a standard curve; mixing the aqueous solution of the Prussian blue nanoscale enzyme, the chromogenic substrate, the peroxide and a sample to be measured, water bath reaction, measuring the absorbance at 650-655 nm, and calculating the tannic acid concentration in the sample to be measured according to the standard curve. The detection method constructed by the application is simple in operation, high in sensitivity and good in selectivity, can be accurately and quickly used for quantitative analysis of tannic acid in tobacco, and has a good application prospect in tobacco quality evaluation and related fields.

The invention discloses an ELISA detection kit for detecting forest encephalitis virus envelope E protein, and relates to the technical field of kits. The kit comprises a pre-coated elisa plate, an elisa antibody, a TBEV E protein quantitative standard substance, a sample diluent, a 20-time concentrated washing solution, a chromogenic substrate A, a chromogenic substrate B, a stop buffer, a plate sealing film and a drying agent. According to the present invention, the totivirus specific correlation can be identified, and the method can be used for the preparation of the kit quality control product so as to achieve the quantitative detection, and is suitable for the E protein preparation and the identification of the specific virus protein monoclonal antibody.

The application relates to a silicon-based supported gold / platinum bimetallic nanocomposite material and a preparation method and application thereof, and belongs to the field of tannic acid detection materials, and comprises the following steps: preparing silica balls, preparing dendritic silica balls modified by 3-aminopropyl triethoxysilane, and preparing dendritic silicon nanoballs loaded with gold / platinum nanoballs. The preparation method is simple, economical, environment-friendly and high in yield, and is convenient for realizing industrialized development. The silicon-based supported gold / platinum bimetallic nanocomposite material is uniform in size, stable in morphology, has strong oxidase-like catalytic activity and good biocompatibility, and has the abilities of in-vitro tannic acid content detection and antibiosis. The silicon-based supported gold / platinum bimetallic nanocomposite material can be applied to colorimetric method and photothermal method double-mode tannic acid content detection, is simple to operate, is simple in system composition, is composed of only materials and chromogenic substrates, and can complete accurate tannic acid detection without the participation of large instruments and other reagents, and is convenient and fast.

The application belongs to the technical field of colorimetric detection, and particularly relates to application of a high-entropy sulfide nano-enzyme material in colorimetric analysis and detection of sulfadiazine. The application uses metal nitrates of Fe, Co, Cu, Cr and Zr and TAA as raw materials to synthesize a five-element high-entropy sulfide nano-enzyme material (F3CZ-48). The F3CZ-48 is used as a colorimetric analysis and detection method with peroxidase activity based on tetramethyl benzidine (TMB) as a chromogenic substrate, and a new method for detecting sulfadiazine (SD) is established. The F3CZ-48 has high stability, easy storage, low cost, excellent catalytic performance and rich reaction sites. By fitting the change of UV-Vis intensity with the concentration of SD, a linear calibration curve with a correlation coefficient of 0.993 is obtained, and the detection limit is 0.121 muM (S / N=3). The material has good sensitivity, selectivity and reproducibility for colorimetric detection of SD, and provides a new scheme for designing a new catalyst for detecting SD.

This invention discloses a detection kit technology, specifically a detection kit and method for novoarginine, comprising a universal sample processing solution, a novoarginine-specific probe, fluorescently labeled streptavidin, washing solution, chromogenic substrate solution, stop solution, novoarginine standard, and a reaction microplate. The universal sample processing solution is composed of acetonitrile, potassium dihydrogen phosphate buffer, and disodium ethylenediaminetetraacetate in a volume ratio of 3:6:1, with the potassium dihydrogen phosphate buffer concentration being 0.1 mol / L and the disodium ethylenediaminetetraacetate concentration being 0.05 mol / L. The novoarginine-specific probe is a biotin-labeled novoarginine monoclonal antibody at a concentration of 1 mg / mL. The surface of the reaction microplate is coated with novoarginine hapten. This invention, by developing a universal sample processing solution adaptable to different matrix samples, eliminates the need for customized pretreatment procedures, significantly improving the versatility of the kit and solving the cumbersome problem of requiring separate processing for different samples.