96 results about "Chromogenic Substrates" patented technology

The invention discloses a method for detecting antioxidants and polyphenols in food by constructing a colorimetric sensing array based on monatomic iron nano-enzyme, and belongs to the field of nanotechnology and colorimetric sensing. The traditional monatomic nano enzyme shows limited enzyme-like activity and substrate specificity, and is difficult to meet the comprehensive requirements of detecting various antioxidants and polyphenols in food. In the research, a novel nano-enzyme system (sdsFeN (at) G) of monatomic iron nano-enzyme is developed. The SdsFeN (at) G shows excellent enzymatic activity, and a three-channel colorimetric sensing array is constructed by utilizing inhibition of different antioxidants and polyphenols on oxidation of three chromogenic substrates, so that simple, convenient and reliable identification and detection of five substances (tert-butylhydroquinone, butylated hydroxyanisole, propyl gallate, catechol and hydroquinone) in food are realized.

The invention provides a colorimetric / fluorescent dual-mode sensor, a preparation method of the colorimetric / fluorescent dual-mode sensor and application of the colorimetric / fluorescent dual-mode sensor in histamine detection. The colorimetric / fluorescent dual-mode sensor comprises a horseradish peroxidase-loaded metal organic framework (HRP-AIE-MOF), hydrogen peroxide and a chromogenic substrate o-phenylenediamine (OPD), horse radish peroxidase (HRP) in the HRP-coated AIE-MOF can oxidize colorless OPD into yellow 2, 3-diaminophenazine (DAP), an ultraviolet absorption peak is generated at 425 nm, a strong fluorescence emission peak is generated at 555 nm, the HRP-coated AIE-MOF has a strong fluorescence emission peak at 450 nm, and the HRP-coated AIE-MOF and a fluorescence signal of the DAP can form a ratio fluorescence sensor. According to the invention, the excellent catalytic ability of natural enzyme and the luminescence characteristic of AIE-MOF are integrated in one system, and high-sensitivity histamine detection is realized.

The invention discloses a method for detecting organophosphorus pesticides through a colorimetric sensor array, and relates to the technical field of organophosphorus pesticide detection and separation. The detection effect is improved; comprising the following steps: synthesizing a ZrMOF (at) Pt (at) ZrMOF composite material with a hierarchical structure; catalyzing an enzymatic reaction between a chromogenic substrate and hydrogen peroxide by using the composite material; and an organophosphorus pesticide identification mode is constructed through multi-channel colorimetric signal change. The ZrMOF (at) Pt (at) ZrMOF composite material with a three-layer structure (inner-layer ZrMOF, middle-layer PtNPs and outer-layer ZrMOF) is used as a sensing element, the inner-layer ZrMOF provides a larger anchoring surface area for PtNPs and reduces aggregation, and the outer-layer MOF further stabilizes PtNPs and promotes charge transfer in a catalytic reaction process, so that the composite material shows higher peroxidase-like activity, and the sensing element has a good application prospect. The affinity to a substrate is good.

The invention belongs to the technical field of sensors, and relates to an array sensor based on cerium-based metal organic framework nano-enzyme and detection application of perfluoroalkyl compounds. The array sensor is a Ce-MOF triple enzyme activity array sensor and is provided with a plurality of mutually independent sensing channels; the sensing channel contains a Ce-MOF nano enzyme and a chromogenic substrate; the chromogenic substrates comprise a first chromogenic substrate, a second chromogenic substrate and a third chromogenic substrate, the first chromogenic substrate comprises 3, 3 ', 5, 5'-tetramethyl benzidine, the second chromogenic substrate comprises 2, 4-diaminophenol and 4-aminoantipyrine, and the third chromogenic substrate comprises xanthine, purine oxidase and nitrogen blue tetrazole. The MOF nano-enzyme array sensor constructed by the invention has relatively high sensitivity and selectivity in identification and distinguishing of PFASs compounds, not only can accurately distinguish single PFASs compounds, but also can perform quantitative detection, and has good response to PFASs with different concentrations.

The invention discloses a preparation method of photoresponse COFs coated CeO2 nano-enzyme, and relates to the field of nano-materials. According to the invention, FePc, TAPP and 2, 5-dihydroxy-1, 4-phthalaldehyde are used for creating a core-shell material CeO2 (at) FePC / por-COF of photoresponse peroxidase based on COFs (Covalent Organic Frameworks), and the core-shell material CeO2 (at) FePC / por-COF is used for preparing the photoresponse peroxidase based on COFs. According to the invention, the highly ordered structural characteristic of the COFs material is utilized, so that the problem of fluorescence quenching caused by aggregation of traditional micromolecule photosensitizers (such as porphyrin, phthalocyanine and the like) in the use process is effectively solved. According to the present invention, the photoresponsive peroxidase activity is significant, and the chromogenic substrate of tetramethyl benzidine (TMB) can be effectively catalyzed; fePc is integrated into a COFs structure, so that the photoresponse activity of the COFs under near-infrared 808 nm is enhanced, and the COFs have excellent reactive oxygen species (ROS) generation capacity, and have great potential in the aspects of photodynamic therapy, antibacterial treatment, cell imaging and the like in biomedical application.

The invention provides a V2O5-NC nano-enzyme as well as preparation and application thereof. The V2O5-NC nano-enzyme is dark green powder and has a stacked layered structure, the specific surface area is 16-18m < 2 > / g, mesoporous distribution is realized, the average particle size is 20-25nm, and the V2O5-NC nano-enzyme has catalase-like activity. The wide-interlayer-spacing V2O5-NC nano-enzyme based on C-O bond bridging prepared by the invention has relatively good stability, can be prepared on a large scale, is convenient to store, has good affinity to catalytic chromogenic substrates, has high stability, and has universality for the chromogenic reaction of the catalytic chromogenic substrates (TMB, ABTS, DAB and OPD). The V2O5-NC nano-enzyme disclosed by the invention is used for analyzing five organophosphorus pesticides (parathion-methyl, glyphosate, phoxim, methamidophos and chlorpyrifos), has good pesticide recognition capability and interference resistance, and also has the advantages of wide linear range, low detection limit, simplicity and convenience in operation, low detection cost and the like.

The invention relates to a digital immunodetection analysis method for accurate quantification of protein. Specifically, the invention provides a digital immunodetection analysis method for accurate quantification of protein, which comprises the following steps: (s1) providing a water phase mixture; (s2) mixing the aqueous phase mixture with a chromogenic substrate and an oil phase to form a first mixture; (s3) emulsifying the first mixture to form a dispersed and uniformly mixed water-in-oil second mixture; wherein the second mixture contains a first micro-droplet and a second micro-droplet; the first micro-droplet is a micro-droplet containing a positive signal compound; the second micro-droplet is a micro-droplet containing a negative signal compound; and (s4) determining the first micro-droplets to obtain the number of the first micro-droplets so as to obtain a quantitative detection result of the protein.

The invention provides an ascorbic acid high-sensitivity colorimetric sensor as well as a preparation method and application thereof, and aims to solve the problem of low sensitivity of an MOFs (Metal-Organic Frameworks) nano material in ascorbic acid detection. Ce-UiO-66-NH2 nano enzyme particles can catalyze O2 to oxidize TMB (Tetramethylbenzidine) to generate a blue product (TMBOX) under an acidic condition; based on the principle that the absorbance of a mixed solution is reduced due to the fact that the ascorbic acid can react with TMBOX, the high-sensitivity ascorbic acid colorimetric sensor composed of Ce-UiO-66-NH2 nano-enzyme particles with the diameter of 10-50 nm and a chromogenic substrate 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) is constructed. Compared with MnO2, Pt-BGP NCs, CeO2 (at) PTA HNS and Co-PB NCs, the prepared ascorbic acid high-sensitivity colorimetric sensor has the advantages that the reaction time is shorter, and the content of ascorbic acid can be rapidly measured within 15 seconds. Based on the principle, the invention further provides a portable colorimetric test paper for visually detecting the ascorbic acid, and the ascorbic acid in the liquid lemon water can be semi-quantitatively detected by naked eyes within 30 seconds.

The invention relates to the technical field of culture media, and particularly discloses a candida chromogenic culture medium as well as a preparation method and application thereof. The candida chromogenic culture medium disclosed by the invention is prepared from the following components in concentration: 10 to 11 g / L of peptone, 12 to 17 g / L of agar, 7 to 9 g / L of glucose, 1 to 2 g / L of chromogenic substrate and 0.4 to 0.6 g / L of bacteriostatic agent, the chromogenic substrate is formed by mixing heavy 4-nitrophenyl-N-acetyl-beta-D-galactosamine and 4-methylumbelliferone-beta-D-N-acetyl galactosamine, and the chromogenic substrate is formed by mixing heavy 4-nitrophenyl-N-acetyl-beta-D- The bacteriostatic agent is prepared by mixing chloramphenicol, taurocholic acid and nalidixic acid. When being used for detecting and identifying candida albicans, candida tropicalis, candida krusei and candida glabrata, the chromogenic culture medium provided by the invention has strong specificity, short identification time and wide practical application value.

The present invention provides a kit for detecting tuberculosis antibodies. The kit includes a sample diluent, an enzyme conjugate reagent, a chromogenic substrate reagent, a concentrated washing solution reagent, and a detection cassette. The detection cassette includes a sampler and a reaction part. A reaction chamber is provided in the reaction part, a test strip is provided in the reaction chamber, and a quality control area and a detection area are provided on the test strip. A liquid inlet is provided on one side of the reaction chamber, and a liquid outlet is provided on the side of the reaction chamber away from the liquid inlet; a second liquid inlet is provided at the top of the sampler, and a second liquid outlet is provided on the side wall at the bottom of the sampler. The second liquid outlet matches the size of the liquid inlet. The sampler and the reaction part are connected by plugging to make the second liquid outlet communicate with the liquid inlet. By setting the detection cassette as a sampler and a reaction part, the sampling and the reaction are spatially isolated. The sampling is simple and easy to operate. Whether it is the sampling or the reaction stage, the solution is in a closed space, which can effectively prevent the phenomenon that the solution is contaminated by the outside world and causes inaccurate detection.

The present application relates to the technical field of biotechnology, and particularly relates to a D-dimer chemiluminescence assay kit. The present application provides a D-dimer chemiluminescence assay kit, which comprises a magnetic particle suspension (immunomagnetic beads) coated with D-dimer antibody 1, alkaline phosphatase-labeled D-dimer antibody 2 (enzyme-labeled antibody) and a calibrant. The immunomagnetic beads and the enzyme-labeled antibody are prepared by chemical coupling and stored in a buffer containing a soluble high molecular material, which can effectively improve the detection sensitivity and repeatability. The kit can be used in combination with a substrate solution containing a chromogenic substrate such as adamantane and its derivative AMPPD or APS-5 for a full-automatic immune test system, and the content of D-dimer in plasma or whole blood can be determined by a chemiluminescence instrument. The kit has high detection sensitivity and good repeatability.

A class of nanowires that possess enzyme-like catalytic activities, which are termed as “enzyme mimics”. The morphologies of such enzyme mimics are nanoscale wires (i.e., nanowires) of various lengths, diameters, and degrees of branching and bending. The composition of such enzyme mimics may contain various elements, but at least one is a platinum-group metal [including platinum (Pt), iridium (Ir), rhodium (Rh), palladium (Pd), and / or ruthenium (Ru)]. The nanowires as enzyme mimics can efficiently catalyze multiple chemical reactions that are typically catalyzed by natural enzymes (such as peroxidase, catalase, oxidase, and superoxide dismutase). For example, the nanowires can act as peroxidase mimics and catalyze the oxidation of chromogenic substrates by oxidizing agents (e.g., hydrogen peroxide), yielding colored products. In addition, disclosed here include the applications of such enzyme mimics in biosensing systems, wherein the enzyme mimics arc conjugated to bioreceptors (e.g., antibodies) and are used as labels or reporters.

The invention discloses a colorimetric array sensing analysis method for identifying a flavonoid antioxidant, belongs to the technical field of detection, and particularly relates to a flavonoid antioxidant identification technology. The method disclosed by the invention is based on NiCo-LDH, H2O2 is introduced to construct a reaction system of three different chromogenic substrates, and five flavonoid antioxidants are identified by a colorimetric array sensing method. The method and colorimetric array sensing have the characteristic of high throughput, can detect a plurality of samples at the same time, greatly improve the detection efficiency, quickly screen the flavonoid derivatives with high antioxidant activity, lay a foundation for drug research and development and product development, and show better prospects in the fields of medicine, food science and the like.

The invention discloses a rapid selective detection and monitoring culture medium for candida auricula as well as a preparation method and application of the rapid selective detection and monitoring culture medium, and belongs to the technical field of microbiological detection. The culture medium comprises the following components: peptone, yeast extract powder, glucose, agar, a selective inhibitor, a chromogenic substrate and auxiliary components, the chromogenic substrate comprises the following components: dulcitol and X-Gal, the culture medium disclosed by the invention is added with chloramphenicol and ciclopirox olamine to inhibit infectious microbes, and a chromogenic system of dulcitol and X-Gal realizes specific detection of candida auricula. The culture medium has the advantages of high selectivity, rapid identification and low cost, and is suitable for clinical sample and environment monitoring.

The application discloses a kind of peroxidase-like CDs@Fe3O4 nanoparticles and its preparation and application, which comprises: carbon source and nitrogen source are reacted by hydrothermal method, and nitrogen-doped carbon dot solution is prepared;Iron source is added to the nitrogen-doped carbon dot solution and stirred, after reaction at 150-300 DEG C, after separation, washing and drying, the peroxidase-like CDs@Fe3O4 nanoparticles are obtained.The CDs@Fe3O4 prepared by the application can catalyze hydrogen peroxide decomposition to produce hydroxyl radical to induce color change of chromogenic substrate, and Fe 3+ The linear response to chromogenic substrate is realized, and the visual monitoring of the damage depth of the protective layer of metal parts is realized.In the monitoring process, after the protective layer is slightly damaged, the CDs@Fe3O4 is exposed to make the chromogenic substrate develop color, and finally the protective layer is completely damaged to expose the metal, which can also induce the chromogenic substrate to develop color.At the same time, the application provides a convenient colorimetric method for detecting coating damage and metal corrosion using Python program.The method uses color difference as an index, and provides a simple and effective method for evaluating the degree of coating damage and metal corrosion.

The invention discloses a tumor necrosis factor alpha detection kit using a chemiluminescence immunoassay technology, and relates to the technical field of detection kits. An anti-human tumor necrosis factor alpha antibody is used for coating a microporous ELISA plate, after a standard substance is added, the tumor necrosis factor alpha can be tightly combined with the coating antibody, then a detection antibody is added to form a stable antibody-antigen-enzyme-labeled antibody compound, free components are thoroughly removed through plate washing operation, then a chromogenic substrate TMB is added, and the detection sensitivity is high. The TMB is blue under the catalysis of a detection antibody, finally, the TMB is converted into yellow after a stop buffer is added, the depth of the color is positively correlated with the concentration of the tumor necrosis factor alpha in a sample, the absorbance is measured through an enzyme-linked immunosorbent assay instrument, the concentration of the sample can be accurately calculated, and the fixed seat can be prevented from shifting under the cooperation of a connecting shaft and a roller; and by arranging the bearing mechanism, the purposes of stretching out and storing the fixing mechanism can be achieved, and taking, placing and protection are facilitated.

The application provides a method for constructing a double-signal sensing platform based on a non-copper nanometer enzyme AuPt@PBA@L-Pd and application, and a multifunctional nanometer composite material AuPt@L-Pd@PBA (hereinafter referred to as APL) with the effects of an oxidase-like enzyme, a peroxidase-like enzyme and a laccase-like enzyme is prepared. The composite nanometer enzyme has a regular cubic morphology, takes PBA as a base material, loads L-Pd through an amide bond, simultaneously collects the advantages of the MOF material PBA and L-Pd, has multiple enzyme-like effects, and can catalyze different chromogenic substrates. The AuNPs and PtNPs on the surface of APL not only provide good binding sites for antibodies, but also successfully prepare a biological composite probe Ab2@APL, further amplify the sensing signal, and improve the sensitivity of the sensing platform. It is worth mentioning that APL has the effect of a laccase-like enzyme, but does not contain copper ions, and further improves the biocompatibility. Based on the high catalytic activity and multifunctional nanometer enzyme Ab2@APL, a multi-signal sensing platform can be constructed, and the application prospect in more fields is wider.

The invention discloses a method for evaluating the aging degree of solid asphalt. Taking a solid asphalt sample to be detected, and crushing; mixing the crushed solid asphalt sample to be detected with an indicator, and stirring to obtain a reaction solution; wherein the indicator at least comprises a biological enzyme solution and a chromogenic substrate solution; and judging the aging degree of the asphalt according to the color change of the reaction liquid. The solid asphalt aging degree evaluation method provided by the invention is rapid in detection, visual in result and high in evaluation accuracy.

This invention relates to the field of tetracycline detection technology, specifically disclosing an iron porphyrin hydrogen-bonded organic framework nanozyme, its preparation method, and its applications. The iron porphyrin hydrogen-bonded organic framework nanozyme comprises an iron porphyrin hydrogen-bonded organic framework, gold nanoparticles, and a tetracycline nucleic acid aptamer. The gold nanoparticles are non-covalently loaded onto the surface and pores of the iron porphyrin hydrogen-bonded organic framework, and the tetracycline nucleic acid aptamer and gold nanoparticles are connected by coordination covalent bonds. The iron porphyrin hydrogen-bonded organic framework nanozyme exhibits peroxide-like nanozyme activity, catalyzing the oxidation of the chromogenic substrate TMB in the presence of H₂O₂ to detect tetracycline content. By introducing the hydrogen-bonded organic framework and the tetracycline nucleic acid aptamer, the detection specificity is improved, achieving highly sensitive, highly selective, and rapid detection of tetracycline. The colorimetric sensor constructed based on the iron porphyrin hydrogen-bonded organic framework nanozyme provides a new method for rapid and visual detection of tetracycline in feed.

The utility model provides a kind of equipment for dry chemical chromogenic substrate method detection, it is related to dry chemical analysis inspection equipment technical field.The equipment for dry chemical chromogenic substrate method detection, including base, top isolation component and bottom isolation component, is equipped with test hole on base, top isolation component includes transparent observation board, the top of base is equipped with the first through slot being communicated with test hole, transparent observation board is set in the first through slot, and can be slid into test hole along the first through slot extension direction, and test hole is blocked.Bottom isolation component includes upper plate body and lower plate body, the bottom of base is equipped with the second through slot for sending test paper, second through slot is communicated with test hole, lower plate body is coincident with upper plate body and forms conveying body, test paper groove for placing test paper is equipped on the lower plate body of upper plate body side, conveying body is set in second through slot.The utility model can avoid test paper exposure in external environment, greatly improve the accuracy of detection result.

The present invention relates to a method for detecting pyrophosphate and alkaline phosphatase based on metal-based silicate nanozymes, comprising: (1) using silica nanoparticles as a template and adding one or more of iron salts, manganese salts, copper salts, zinc salts, nickel salts, cerium salts, and cobalt salts to incorporate one or more of iron, manganese, copper, zinc, nickel, cerium, and cobalt ions to obtain metal-based silicate nanozymes; (2) the metal-based silicate nanozymes exhibit peroxidase-like activity and catalyze the oxidation and color development of a chromogenic substrate in the presence of H2O2; (3) in the presence of pyrophosphate (PPi), PPi will complex with the metal ions in the metal-based silicate nanozymes, thereby inhibiting their peroxidase-like activity, and the colorless chromogenic substrate cannot be oxidized and developed; (4) alkaline phosphatase (ALP) can hydrolyze PPi to restore the peroxidase-like activity of the metal-based silicate nanozymes, and the colorless chromogenic substrate develops color; thus, metal-based silicate nanozymes with high specificity, stability, and sensitivity are prepared to achieve rapid and simple detection of PPi and ALP.

Disclosed herein is a system for self-administering an infection culture test, the system including a housing having a cover and a compartment for storing a bacterial culture and to facilitate bacterial growth, wherein the compartment includes a pad having a partitioning element attached to a film, wherein the partitioning element separates a dry chromogenic medium into regions, wherein the medium is coated with a growth medium and a chromogenic substrate, and a transparent cover to hermetically seal the compartment, and one or more smart devices configured to record one or more images of the bacteria within the apparatus and provide a determination whether an infection is present and to identify what specific bacteria is present.

The application belongs to the technical field of sensors and relates to an array sensor based on cerium-based metal organic framework nanoscale enzyme and detection application of perfluoroalkyl compounds. The array sensor is a Ce-MOF triple enzyme activity array sensor and has multiple independent sensing channels. The sensing channels contain Ce-MOF nanoscale enzyme and chromogenic substrate. The chromogenic substrate includes first, second and third chromogenic substrates. The first chromogenic substrate includes 3,3',5,5'-tetramethylbenzidine. The second chromogenic substrate includes 2,4-diamino phenol and 4-aminoantipyrine. The third chromogenic substrate includes xanthine, xanthine oxidase and nitrogen blue tetrazolium. The MOF nanoscale enzyme array sensor constructed by the application has high sensitivity and selectivity in the identification and differentiation of PFASs compounds. The array sensor can not only accurately distinguish single PFASs compounds but also quantitatively detect the compounds, and has good response to PFASs with different concentrations.

The invention discloses a wearable sensor-minimally invasive diabetes monitoring patch and a preparation method thereof, and belongs to the technical field of biomedicine, the patch comprises a microneedle array module and a sensing unit; the microneedle array module is composed of a plurality of microneedles arranged in an array; the sensing unit is loaded in the microneedle, and the sensing unit comprises a multi-shell metal organic framework cascade enzyme system and a chromogenic substrate; the multi-shell metal organic framework cascade enzyme system is of a double-layer structure composed of an inner core and an outer shell, the inner core is a metal organic framework packaged with glucose oxidase and modified with carboxyl functional groups, and the outer shell is a metal organic framework packaged with horse radish peroxidase. And 2, 2-nitrogen-bis (3-ethylbenzothiazoline-6-sulfonic acid) is taken as a chromogenic substrate. According to the patch, the catalytic efficiency and the detection accuracy are improved, the stability is improved, the storage and transportation cost is reduced, the low detection limit can be achieved, the requirement for instant blood glucose detection is met, the size is small, and the cost is low.

The invention discloses a preparation method of cerium-based nano enzyme, which comprises the following steps: reacting cerium salt and zinc salt with 2-methylimidazole in methanol, then loading into a high-pressure reaction kettle to prepare a Ce-MOF precursor, then calcining the precursor in a tubular furnace to obtain powder, and finally pickling the powder to obtain the cerium-based nano enzyme. The prepared cerium-based nano-enzyme is used for uric acid detection, can replace natural enzyme for quantitative analysis, and has the advantages of high sensitivity, low detection limit and better selectivity and stability. The invention also provides a quantitative analysis method for detecting uric acid, which comprises the following steps: reacting uric acid with uricase to generate hydrogen peroxide, then catalyzing the hydrogen peroxide by the cerium-based nano-enzyme to oxidize a chromogenic substrate, and finally quantifying according to a color B value, absorbance or temperature.

The invention relates to the technical field of early risk assessment of gynecological tumors, and discloses a tumor risk assessment system based on an epithelial ion channel protein expression nanoprobe. Comprising a detection composition which is prepared into a secondary antibody containing a chromophoric enzyme label or a fluorescent substance label, a chromogenic substrate and a buffer solution; the diagnosis kit is configured to contain the detection composition and provide an integrated micro-fluidic chip for bearing a sample; the computer equipment is configured to obtain channel activity change data monitored by the micro-fluidic chip in real time through a multi-time impedance method, calculate a real-time sampling error based on the multiple groups of channel activity data, adjust sampling parameters to enable the sampling error to reach a continuous minimum variance, and optimize the color development signal or the development signal; and carrying out fusion analysis on the optimized protein expression data, imaging evidence and physical and chemical inspection data to generate a gynecological tumor risk collaborative estimation result.

This invention provides a colorimetric / fluorescence dual-mode nanozyme aptamer sensor, its preparation method, and its application in bisphenol A (BPA) detection. The sensor comprises a metal-organic framework nanozyme loaded with a BPA aptamer, hydrogen peroxide, and a chromogenic substrate, TMB. Cu / Zr-MOF oxidizes the colorless TMB to blue oxTMB, producing a UV absorption peak at 652 nm and a fluorescence emission peak at 415 nm. Modification with the BPA aptamer enhances the specific recognition ability of BPA, while simultaneously inhibiting the enzyme activity of Cu / Zr-MOF, leading to weakened colorimetric and fluorescence signals. In the presence of BPA, the aptamer specifically binds to the target and dissociates from the nanozyme surface, restoring enzyme activity and enhancing colorimetric and fluorescence signals. This invention integrates the excellent catalytic ability of nanozymes with the specific recognition ability of aptamers into a single system, achieving highly sensitive BPA detection.

The invention relates to the technical field of detection, and relates to a single-molecule protein detection method based on a microarray chip and a chemiluminescence immunoassay technology. An antigen-antibody complex reaction solution is randomly segmented into tens of thousands of microarray holes with the single-hole volume at the pL level by utilizing a microarray chip containing 0.5-200 thousands of micropores with the diameter of 5-30 [mu] m. Each micropore is used as an independent reaction unit for detection and comprises one or zero antigen-antibody compounds, a chromogenic substrate or exciting light is added to collect signal data in all the micropores, and the number of all the antigen-antibody compounds in a reaction system is directly and quantitatively detected according to a Poisson distribution calculation formula. And calculating the concentration of the antigen captured in the microarray chip. The method disclosed by the invention has the advantages of small background signal interference, high sensitivity, specificity, accuracy and precision, simplicity and rapidness in operation, low cost and obvious advantages in the technical field of single molecule detection, and the lower limit of detection reaches fg / ml.

A disposable bioassay diagnostic cartridge for performing enzyme-linked immunosorbent assay for a single patient in whole blood and plasma where the cartridge has a plurality of wells. The cartridge includes a pipette tip with internal sidewalls disposed in one well of the plurality of wells, the internal sidewalls being pretreated and coated with antibodies, a reagent mixture disposed in another well of the plurality of wells, the reagent mixture comprising at least one reagent selected from the group consisting of pH stabilizer, ionic catalyst control agent, ionic intracellular inhibitory control agent, detergent, negative control agent, chelating agent, and biocide preservative, antibody, and conjugate stabilizing agent. At least a chromogenic substrate and visualizing reagent disposed in an integrated cuvette of the plurality of wells. It also includes the detection of the hemoglobin amount in the blood sample so a correction for plasma amount in a sample can be performed.

The invention discloses a three-mode biosensor for detecting glutathione and copper ions and a construction method of the three-mode biosensor, and belongs to the field of biosensors. According to the sensor, ultraviolet spectrophotometry, Raman and a smart phone are combined, TMB is taken as a chromogenic substrate, Ag / MgMn2O4 and TMB are added into a NaAc buffer solution by utilizing the enzyme activity of Ag / MgMn2O4 oxide, GSH and Cu < 2 + > are detected, and when GSH exists, TMB oxide is inhibited to be TMbox < x >; and after Cu < 2 + > is added, the colorless TMB is re-oxidized into blue TMbox < ox >. The ultraviolet-Raman-smart phone three-mode biosensor for detecting GSH and Cu < 2 + > based on Ag / MgMn2O4 nano-enzyme is constructed, an ultra-sensitive new technology is provided for rapid and accurate detection of GSH and Cu < 2 + >, and the biosensor is especially suitable for concentration detection of GSH in urine and concentration detection of Cu < 2 + > in environmental lake water.