69 results about "Chromogenic Substrates" patented technology

The invention belongs to the technical field of sensors, and relates to an array sensor based on cerium-based metal organic framework nano-enzyme and detection application of perfluoroalkyl compounds. The array sensor is a Ce-MOF triple enzyme activity array sensor and is provided with a plurality of mutually independent sensing channels; the sensing channel contains a Ce-MOF nano enzyme and a chromogenic substrate; the chromogenic substrates comprise a first chromogenic substrate, a second chromogenic substrate and a third chromogenic substrate, the first chromogenic substrate comprises 3, 3 ', 5, 5'-tetramethyl benzidine, the second chromogenic substrate comprises 2, 4-diaminophenol and 4-aminoantipyrine, and the third chromogenic substrate comprises xanthine, purine oxidase and nitrogen blue tetrazole. The MOF nano-enzyme array sensor constructed by the invention has relatively high sensitivity and selectivity in identification and distinguishing of PFASs compounds, not only can accurately distinguish single PFASs compounds, but also can perform quantitative detection, and has good response to PFASs with different concentrations.

The invention provides an ascorbic acid high-sensitivity colorimetric sensor as well as a preparation method and application thereof, and aims to solve the problem of low sensitivity of an MOFs (Metal-Organic Frameworks) nano material in ascorbic acid detection. Ce-UiO-66-NH2 nano enzyme particles can catalyze O2 to oxidize TMB (Tetramethylbenzidine) to generate a blue product (TMBOX) under an acidic condition; based on the principle that the absorbance of a mixed solution is reduced due to the fact that the ascorbic acid can react with TMBOX, the high-sensitivity ascorbic acid colorimetric sensor composed of Ce-UiO-66-NH2 nano-enzyme particles with the diameter of 10-50 nm and a chromogenic substrate 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) is constructed. Compared with MnO2, Pt-BGP NCs, CeO2 (at) PTA HNS and Co-PB NCs, the prepared ascorbic acid high-sensitivity colorimetric sensor has the advantages that the reaction time is shorter, and the content of ascorbic acid can be rapidly measured within 15 seconds. Based on the principle, the invention further provides a portable colorimetric test paper for visually detecting the ascorbic acid, and the ascorbic acid in the liquid lemon water can be semi-quantitatively detected by naked eyes within 30 seconds.

The invention relates to the technical field of culture media, and particularly discloses a candida chromogenic culture medium as well as a preparation method and application thereof. The candida chromogenic culture medium disclosed by the invention is prepared from the following components in concentration: 10 to 11 g / L of peptone, 12 to 17 g / L of agar, 7 to 9 g / L of glucose, 1 to 2 g / L of chromogenic substrate and 0.4 to 0.6 g / L of bacteriostatic agent, the chromogenic substrate is formed by mixing heavy 4-nitrophenyl-N-acetyl-beta-D-galactosamine and 4-methylumbelliferone-beta-D-N-acetyl galactosamine, and the chromogenic substrate is formed by mixing heavy 4-nitrophenyl-N-acetyl-beta-D- The bacteriostatic agent is prepared by mixing chloramphenicol, taurocholic acid and nalidixic acid. When being used for detecting and identifying candida albicans, candida tropicalis, candida krusei and candida glabrata, the chromogenic culture medium provided by the invention has strong specificity, short identification time and wide practical application value.

The present application relates to the technical field of biotechnology, and particularly relates to a D-dimer chemiluminescence assay kit. The present application provides a D-dimer chemiluminescence assay kit, which comprises a magnetic particle suspension (immunomagnetic beads) coated with D-dimer antibody 1, alkaline phosphatase-labeled D-dimer antibody 2 (enzyme-labeled antibody) and a calibrant. The immunomagnetic beads and the enzyme-labeled antibody are prepared by chemical coupling and stored in a buffer containing a soluble high molecular material, which can effectively improve the detection sensitivity and repeatability. The kit can be used in combination with a substrate solution containing a chromogenic substrate such as adamantane and its derivative AMPPD or APS-5 for a full-automatic immune test system, and the content of D-dimer in plasma or whole blood can be determined by a chemiluminescence instrument. The kit has high detection sensitivity and good repeatability.

The invention discloses a colorimetric array sensing analysis method for identifying a flavonoid antioxidant, belongs to the technical field of detection, and particularly relates to a flavonoid antioxidant identification technology. The method disclosed by the invention is based on NiCo-LDH, H2O2 is introduced to construct a reaction system of three different chromogenic substrates, and five flavonoid antioxidants are identified by a colorimetric array sensing method. The method and colorimetric array sensing have the characteristic of high throughput, can detect a plurality of samples at the same time, greatly improve the detection efficiency, quickly screen the flavonoid derivatives with high antioxidant activity, lay a foundation for drug research and development and product development, and show better prospects in the fields of medicine, food science and the like.

The invention discloses a rapid selective detection and monitoring culture medium for candida auricula as well as a preparation method and application of the rapid selective detection and monitoring culture medium, and belongs to the technical field of microbiological detection. The culture medium comprises the following components: peptone, yeast extract powder, glucose, agar, a selective inhibitor, a chromogenic substrate and auxiliary components, the chromogenic substrate comprises the following components: dulcitol and X-Gal, the culture medium disclosed by the invention is added with chloramphenicol and ciclopirox olamine to inhibit infectious microbes, and a chromogenic system of dulcitol and X-Gal realizes specific detection of candida auricula. The culture medium has the advantages of high selectivity, rapid identification and low cost, and is suitable for clinical sample and environment monitoring.

The application discloses a kind of peroxidase-like CDs@Fe3O4 nanoparticles and its preparation and application, which comprises: carbon source and nitrogen source are reacted by hydrothermal method, and nitrogen-doped carbon dot solution is prepared;Iron source is added to the nitrogen-doped carbon dot solution and stirred, after reaction at 150-300 DEG C, after separation, washing and drying, the peroxidase-like CDs@Fe3O4 nanoparticles are obtained.The CDs@Fe3O4 prepared by the application can catalyze hydrogen peroxide decomposition to produce hydroxyl radical to induce color change of chromogenic substrate, and Fe 3+ The linear response to chromogenic substrate is realized, and the visual monitoring of the damage depth of the protective layer of metal parts is realized.In the monitoring process, after the protective layer is slightly damaged, the CDs@Fe3O4 is exposed to make the chromogenic substrate develop color, and finally the protective layer is completely damaged to expose the metal, which can also induce the chromogenic substrate to develop color.At the same time, the application provides a convenient colorimetric method for detecting coating damage and metal corrosion using Python program.The method uses color difference as an index, and provides a simple and effective method for evaluating the degree of coating damage and metal corrosion.

The application provides a method for constructing a double-signal sensing platform based on a non-copper nanometer enzyme AuPt@PBA@L-Pd and application, and a multifunctional nanometer composite material AuPt@L-Pd@PBA (hereinafter referred to as APL) with the effects of an oxidase-like enzyme, a peroxidase-like enzyme and a laccase-like enzyme is prepared. The composite nanometer enzyme has a regular cubic morphology, takes PBA as a base material, loads L-Pd through an amide bond, simultaneously collects the advantages of the MOF material PBA and L-Pd, has multiple enzyme-like effects, and can catalyze different chromogenic substrates. The AuNPs and PtNPs on the surface of APL not only provide good binding sites for antibodies, but also successfully prepare a biological composite probe Ab2@APL, further amplify the sensing signal, and improve the sensitivity of the sensing platform. It is worth mentioning that APL has the effect of a laccase-like enzyme, but does not contain copper ions, and further improves the biocompatibility. Based on the high catalytic activity and multifunctional nanometer enzyme Ab2@APL, a multi-signal sensing platform can be constructed, and the application prospect in more fields is wider.

This invention relates to the field of tetracycline detection technology, specifically disclosing an iron porphyrin hydrogen-bonded organic framework nanozyme, its preparation method, and its applications. The iron porphyrin hydrogen-bonded organic framework nanozyme comprises an iron porphyrin hydrogen-bonded organic framework, gold nanoparticles, and a tetracycline nucleic acid aptamer. The gold nanoparticles are non-covalently loaded onto the surface and pores of the iron porphyrin hydrogen-bonded organic framework, and the tetracycline nucleic acid aptamer and gold nanoparticles are connected by coordination covalent bonds. The iron porphyrin hydrogen-bonded organic framework nanozyme exhibits peroxide-like nanozyme activity, catalyzing the oxidation of the chromogenic substrate TMB in the presence of H₂O₂ to detect tetracycline content. By introducing the hydrogen-bonded organic framework and the tetracycline nucleic acid aptamer, the detection specificity is improved, achieving highly sensitive, highly selective, and rapid detection of tetracycline. The colorimetric sensor constructed based on the iron porphyrin hydrogen-bonded organic framework nanozyme provides a new method for rapid and visual detection of tetracycline in feed.

The utility model provides a kind of equipment for dry chemical chromogenic substrate method detection, it is related to dry chemical analysis inspection equipment technical field.The equipment for dry chemical chromogenic substrate method detection, including base, top isolation component and bottom isolation component, is equipped with test hole on base, top isolation component includes transparent observation board, the top of base is equipped with the first through slot being communicated with test hole, transparent observation board is set in the first through slot, and can be slid into test hole along the first through slot extension direction, and test hole is blocked.Bottom isolation component includes upper plate body and lower plate body, the bottom of base is equipped with the second through slot for sending test paper, second through slot is communicated with test hole, lower plate body is coincident with upper plate body and forms conveying body, test paper groove for placing test paper is equipped on the lower plate body of upper plate body side, conveying body is set in second through slot.The utility model can avoid test paper exposure in external environment, greatly improve the accuracy of detection result.

Disclosed herein is a system for self-administering an infection culture test, the system including a housing having a cover and a compartment for storing a bacterial culture and to facilitate bacterial growth, wherein the compartment includes a pad having a partitioning element attached to a film, wherein the partitioning element separates a dry chromogenic medium into regions, wherein the medium is coated with a growth medium and a chromogenic substrate, and a transparent cover to hermetically seal the compartment, and one or more smart devices configured to record one or more images of the bacteria within the apparatus and provide a determination whether an infection is present and to identify what specific bacteria is present.

The application belongs to the technical field of sensors and relates to an array sensor based on cerium-based metal organic framework nanoscale enzyme and detection application of perfluoroalkyl compounds. The array sensor is a Ce-MOF triple enzyme activity array sensor and has multiple independent sensing channels. The sensing channels contain Ce-MOF nanoscale enzyme and chromogenic substrate. The chromogenic substrate includes first, second and third chromogenic substrates. The first chromogenic substrate includes 3,3',5,5'-tetramethylbenzidine. The second chromogenic substrate includes 2,4-diamino phenol and 4-aminoantipyrine. The third chromogenic substrate includes xanthine, xanthine oxidase and nitrogen blue tetrazolium. The MOF nanoscale enzyme array sensor constructed by the application has high sensitivity and selectivity in the identification and differentiation of PFASs compounds. The array sensor can not only accurately distinguish single PFASs compounds but also quantitatively detect the compounds, and has good response to PFASs with different concentrations.

The invention discloses a wearable sensor-minimally invasive diabetes monitoring patch and a preparation method thereof, and belongs to the technical field of biomedicine, the patch comprises a microneedle array module and a sensing unit; the microneedle array module is composed of a plurality of microneedles arranged in an array; the sensing unit is loaded in the microneedle, and the sensing unit comprises a multi-shell metal organic framework cascade enzyme system and a chromogenic substrate; the multi-shell metal organic framework cascade enzyme system is of a double-layer structure composed of an inner core and an outer shell, the inner core is a metal organic framework packaged with glucose oxidase and modified with carboxyl functional groups, and the outer shell is a metal organic framework packaged with horse radish peroxidase. And 2, 2-nitrogen-bis (3-ethylbenzothiazoline-6-sulfonic acid) is taken as a chromogenic substrate. According to the patch, the catalytic efficiency and the detection accuracy are improved, the stability is improved, the storage and transportation cost is reduced, the low detection limit can be achieved, the requirement for instant blood glucose detection is met, the size is small, and the cost is low.

The invention discloses a preparation method of cerium-based nano enzyme, which comprises the following steps: reacting cerium salt and zinc salt with 2-methylimidazole in methanol, then loading into a high-pressure reaction kettle to prepare a Ce-MOF precursor, then calcining the precursor in a tubular furnace to obtain powder, and finally pickling the powder to obtain the cerium-based nano enzyme. The prepared cerium-based nano-enzyme is used for uric acid detection, can replace natural enzyme for quantitative analysis, and has the advantages of high sensitivity, low detection limit and better selectivity and stability. The invention also provides a quantitative analysis method for detecting uric acid, which comprises the following steps: reacting uric acid with uricase to generate hydrogen peroxide, then catalyzing the hydrogen peroxide by the cerium-based nano-enzyme to oxidize a chromogenic substrate, and finally quantifying according to a color B value, absorbance or temperature.

The invention relates to the technical field of early risk assessment of gynecological tumors, and discloses a tumor risk assessment system based on an epithelial ion channel protein expression nanoprobe. Comprising a detection composition which is prepared into a secondary antibody containing a chromophoric enzyme label or a fluorescent substance label, a chromogenic substrate and a buffer solution; the diagnosis kit is configured to contain the detection composition and provide an integrated micro-fluidic chip for bearing a sample; the computer equipment is configured to obtain channel activity change data monitored by the micro-fluidic chip in real time through a multi-time impedance method, calculate a real-time sampling error based on the multiple groups of channel activity data, adjust sampling parameters to enable the sampling error to reach a continuous minimum variance, and optimize the color development signal or the development signal; and carrying out fusion analysis on the optimized protein expression data, imaging evidence and physical and chemical inspection data to generate a gynecological tumor risk collaborative estimation result.

This invention provides a colorimetric / fluorescence dual-mode nanozyme aptamer sensor, its preparation method, and its application in bisphenol A (BPA) detection. The sensor comprises a metal-organic framework nanozyme loaded with a BPA aptamer, hydrogen peroxide, and a chromogenic substrate, TMB. Cu / Zr-MOF oxidizes the colorless TMB to blue oxTMB, producing a UV absorption peak at 652 nm and a fluorescence emission peak at 415 nm. Modification with the BPA aptamer enhances the specific recognition ability of BPA, while simultaneously inhibiting the enzyme activity of Cu / Zr-MOF, leading to weakened colorimetric and fluorescence signals. In the presence of BPA, the aptamer specifically binds to the target and dissociates from the nanozyme surface, restoring enzyme activity and enhancing colorimetric and fluorescence signals. This invention integrates the excellent catalytic ability of nanozymes with the specific recognition ability of aptamers into a single system, achieving highly sensitive BPA detection.

The invention relates to the technical field of detection, and relates to a single-molecule protein detection method based on a microarray chip and a chemiluminescence immunoassay technology. An antigen-antibody complex reaction solution is randomly segmented into tens of thousands of microarray holes with the single-hole volume at the pL level by utilizing a microarray chip containing 0.5-200 thousands of micropores with the diameter of 5-30 [mu] m. Each micropore is used as an independent reaction unit for detection and comprises one or zero antigen-antibody compounds, a chromogenic substrate or exciting light is added to collect signal data in all the micropores, and the number of all the antigen-antibody compounds in a reaction system is directly and quantitatively detected according to a Poisson distribution calculation formula. And calculating the concentration of the antigen captured in the microarray chip. The method disclosed by the invention has the advantages of small background signal interference, high sensitivity, specificity, accuracy and precision, simplicity and rapidness in operation, low cost and obvious advantages in the technical field of single molecule detection, and the lower limit of detection reaches fg / ml.

A disposable bioassay diagnostic cartridge for performing enzyme-linked immunosorbent assay for a single patient in whole blood and plasma where the cartridge has a plurality of wells. The cartridge includes a pipette tip with internal sidewalls disposed in one well of the plurality of wells, the internal sidewalls being pretreated and coated with antibodies, a reagent mixture disposed in another well of the plurality of wells, the reagent mixture comprising at least one reagent selected from the group consisting of pH stabilizer, ionic catalyst control agent, ionic intracellular inhibitory control agent, detergent, negative control agent, chelating agent, and biocide preservative, antibody, and conjugate stabilizing agent. At least a chromogenic substrate and visualizing reagent disposed in an integrated cuvette of the plurality of wells. It also includes the detection of the hemoglobin amount in the blood sample so a correction for plasma amount in a sample can be performed.

The invention discloses a colorimetric sensing array based on hierarchical pore nano-enzyme as well as a construction method and application of the colorimetric sensing array, and belongs to the technical field of analysis and detection. According to the array, two cerium-based MOF nano-enzymes, namely micropore UiO-66 and hierarchical pore HP-UiO-66, are respectively combined with TMB (tetramethylbenzidine) and ABTS (2, 2, 6, 6-tetramethylbenzidine) chromogenic substrates to form a four-channel sensing system. The method comprises the following steps: synthesizing hierarchical pore HP-UiO-66 by a template method; collecting the absorbance of each channel under the characteristic wavelength by using an array, and generating a specific fingerprint spectrum of the to-be-detected object; by combining mode recognition technologies such as linear discriminant analysis and the like, rapid and synchronous distinguishing and quantitative detection of at least eight antioxidants with similar structures such as glycyrrhizic acid and caffeic acid in the traditional Chinese medicine decoction are realized. According to the method, the catalysis and mass transfer efficiency is improved by regulating and controlling the pore structure of the nano-enzyme, the problem of multi-target synchronous recognition in a complex matrix is solved by utilizing array cross response, and the method has the advantages of simplicity and convenience in operation, high flux and good accuracy.

The invention belongs to the technical field of biomedical detection and immunoassay, and particularly discloses a mycobacterium tuberculosis detection probe as well as a preparation method and application thereof. The mycobacterium tuberculosis detection probe provided by the invention comprises a capture probe and a signal probe, the capture probe is magnetic nanoparticles of which the surfaces are fixedly loaded with a mycobacterium tuberculosis specific antibody 1, and the signal probe is a mycobacterium tuberculosis specific antibody 2 of which the surface is marked with a photosensitizer. According to the mycobacterium tuberculosis detection probe disclosed by the invention, a photosensitizer labeling technology is combined with immunoassay, so that high-sensitivity detection of mycobacterium tuberculosis can be realized under a mild condition; under the irradiation of visible light, active oxygen generated by the photosensitizer can catalyze oxidation reaction of various chromogenic substrates, so that rapid colorimetric detection of mycobacterium tuberculosis is realized, and the kit is particularly suitable for the requirements of clinical serum sample detection and the like; in addition, the fluorescent probe also has the advantages of simple preparation process, strong recognition specificity, high detection sensitivity, good stability and reproducibility and the like.

The present invention relates to the field of bioanalysis and detection technology, and specifically to a gold nanoparticle-modified magnetic covalent organic framework material, its preparation method, and application. The gold nanoparticle-modified magnetic covalent organic framework material exhibits both glucose oxidase-like activity and peroxidase-like activity under acidic conditions. In the presence of the substrate glucose, the gold nanoparticle-modified magnetic covalent organic framework material oxidizes the glucose to generate hydrogen peroxide through self-cascade catalysis, and then utilizes the peroxidase-like activity to oxidize the chromogenic substrate TMB to generate oxTMB, which is then colorimetrically detected based on the color depth of the substrate. In addition, the generated oxTMB reacts with the fluorescence of the gold nanoparticle-modified magnetic covalent organic framework material itself to produce an inner filter effect, quenching the autofluorescence, allowing fluorescence detection to be performed based on the fluorescence change. Rapidly detecting glucose content through the colorimetric / fluorescence dual mode provides a new approach for convenient and accurate glucose content detection.

The invention belongs to the technical field of immunological detection, and particularly discloses a monoclonal antibody resisting human monocyte chemoattractant protein 1, a kit containing the monoclonal antibody and application of the monoclonal antibody. The monoclonal antibody is a paired antibody, comprises a 6H11 antibody and a 1F12 antibody, and can specifically recognize human monocyte chemotactic protein 1. A human MCP-1 antibody (6H11) is coated with an elisa plate to form a solid-phase carrier, the solid-phase carrier is combined with an MCP-1 antigen in a biological sample to be detected, an antibody (1F12) is detected through specific enzyme labeling, a solid-phase antibody-antigen-enzyme labeled antibody compound is formed, and detection is performed through intervention of a chromogenic substrate solution. The detection kit provided by the invention adopts a one-step method, a standard product / sample and an enzyme-labeled antibody are added together for reaction during detection, the reaction time does not exceed 1h, the whole experimental operation steps are few, the time is short, the sensitivity is high, and the precision and the accuracy are good.

The invention provides an asphalt carbon-based oxidase-like nano material and a preparation method and application thereof.In the preparation method of the asphalt carbon-based oxidase-like nano material, starting from modification of a coal liquefaction asphalt base material, the asphalt carbon-based oxidase-like nano material is prepared through a series of links of coal liquefaction asphalt activation, metal ion loading, nitrogen doping, heat treatment and the like. And the asphalt carbon-based oxidase-like nano material with high specific surface area, rich porous structure, stable metal active sites and optimized electron distribution is gradually constructed. Through the elaborately designed steps, the material can efficiently catalyze oxidation of a chromogenic substrate without participation of hydrogen peroxide, visual detection of the antioxidant is achieved, and meanwhile it is ensured that the detection method has the remarkable advantages of being low in cost, easy and convenient to operate, high in sensitivity and wide in applicability; and powerful support is provided for technical innovation in the fields of food safety and health monitoring.

The invention provides a FeCoP-NC diatomic nano enzyme as well as a preparation method and application thereof. The used FeCoP-NC nano-enzyme has active sites of FeN3P-CoN3P, the mass fraction of doped P is 0.08%-0.12%, the mass fraction of Fe is 0.25%-0.32%, the mass fraction of Co is 0.5%-0.58%, the FeCoP-NC nano-enzyme is of a dodecahedron structure, the surface area of the FeCoP-NC nano-enzyme is 810-830 m < 2 > / g, the FeCoP-NC nano-enzyme is of a porous structure, and the pore diameter of the FeCoP-NC nano-enzyme is 3-3.5 nm. The FeCoP-NC nano-enzyme disclosed by the invention has excellent oxide-like enzyme activity, and can be used as a catalyst for catalyzing a chromogenic reaction of a chromogenic substrate in a colorimetric sensor.

The invention discloses a recombinant tachypleus amebocyte lyophilized preparation for bacterial endotoxin detection as well as a preparation method and application of the recombinant tachypleus amebocyte lyophilized preparation. The recombinant tachypleus amebocyte lyophilized preparation is obtained by lyophilizing a lyophilized solution, wherein the lyophilized solution comprises the following components: mannitol, cane sugar, polyvinylpyrrolidone, recombinant human albumin, ascorbic acid, sodium chloride, inositol, L-arginine hydrochloride, ethylene glycol diethyl ether diamine tetraacetic acid, Tween 80, Proclin 300, Tris-HCl with the pH value of 7.0-8.0, a recombinant tachypleus amebocyte lyophilized preparation, a chromogenic substrate and a reaction buffer solution. The invention further discloses a preparation method and application of the recombinant tachypleus amebocyte lyophilized preparation. The recombinant tachypleus amebocyte lyophilized preparation is prepared by matching a multi-element synergistic protection system with a seven-stage gradient temperature control freeze-drying process, and can be used for high-sensitivity and high-stability detection of bacterial endotoxin in drugs, medical instruments, biological products and clinical samples.

The application relates to the technical field of biological medicine, and particularly discloses a kit for determining antithrombin III activity, which comprises a first reagent, a second reagent, a third reagent and a diluent; the first reagent at least comprises a first buffer, thrombin, a serine protease inhibitor and a first stabilizer; the second reagent at least comprises a second buffer, a chromogenic substrate and a second stabilizer; the third reagent at least comprises a third buffer, heparin, isopropyl alcohol and a third stabilizer; and the diluent at least comprises physiological saline and a fourth stabilizer. In the kit, the cost is low, the anti-interference capability is strong, the sensitivity is high, the stability is good, and the kit has a wide application prospect.

The application discloses a rivaroxaban concentration determination method based on anti-Xa factor activity, a determination reagent and a preparation method thereof, and relates to the technical field of biology, and comprises reagent R1, reagent R2 and a sample diluent.The reagent R1 comprises Xa factor, a buffer, a stabilizer and inorganic salt.The reagent R2 comprises a chromogenic substrate, a buffer, polyethylene glycol and inorganic salt.The sample diluent comprises a buffer, a stabilizer and inorganic salt and is used for diluting samples with a concentration higher than a preset threshold value.The application remarkably reduces the cost by adopting an optimized formula of bovine factor and domestic raw materials, ensures the detection accuracy of high-concentration samples, and has excellent sensitivity, specificity and stability, realizes a detection performance highly related to a gold standard method, and thus successfully overcomes the technical bottlenecks of high detection cost, limited linear range, inaccurate detection of high-value samples and lack of high-quality domestic reagents in the prior art.

This invention provides a colorimetric / fluorescence dual-mode sensor, its preparation method, and its application in histamine detection. The colorimetric / fluorescence dual-mode sensor comprises a metal-organic framework (HRP@AIE-MOF) loaded with horseradish peroxidase, hydrogen peroxide, and the chromogenic substrate o-phenylenediamine (OPD). The horseradish peroxidase (HRP) in the HRP@AIE-MOF oxidizes colorless OPD to yellow 2,3-diaminophenazine (DAP), producing a UV absorption peak at 425 nm and a strong fluorescence emission peak at 555 nm. The HRP@AIE-MOF also exhibits a strong fluorescence emission peak at 450 nm, which, together with the fluorescence signal of DAP, forms a ratiometric fluorescence sensor. This invention integrates the excellent catalytic ability of a natural enzyme with the luminescent properties of AIE-MOF into a single system, achieving highly sensitive histamine detection.

The invention discloses a green preparation method and application of alginate oligosaccharide nano-silver, ellipsoidal AOS-AgNPs of about 50 nanometers is prepared through an environment-friendly method, the material has oxidase-like activity, and the activity is remarkably enhanced after the material is combined with mercury ions to form Ag-Hg alloy. Based on the characteristic, an AOS-AgNPs-TMB-Hg < 2 + > detection system is studied and constructed, and 3, 3 ', 5, 5'-tetramethyl benzidine (TMB) is adopted as a chromogenic substrate to realize accurate detection of Hg < 2 + > in a water environment. The system shows excellent performance: the affinity to TMB (Tetramethylbenzidine) is high (Km is equal to 0.547 mM, Vmax is equal to 67.98 mu M / min), the detection linear range is wide (5-100 mu M), the detection limit is as low as 10nM, and the system has strong anti-interference capability. The portable paper-based colorimetric sensor is researched and developed by combining a smart phone technology, and the detection range is expanded to 30-200 [mu] M. The technology breaks through the limitation of a traditional detection method, has high sensitivity, operability and portability, has important application value in the fields of environmental monitoring and agricultural product safety assessment, and provides a new solution for mercury pollution prevention and control.

The present invention discloses a kit for accurate fluorescence and colorimetric multimodal detection of African swine fever virus, comprising a 96-well polystyrene plate, African swine fever virus containing P72 protein after being crushed, a blocking agent, a P72 protein capture antibody, a P72 protein monoclonal antibody labeled with a palladium-gold nanodendrite particle mimicking enzyme, a hydrogen peroxide solution (H2O2), an o-phenylenediamine solution, a washing solution, a diluent, and a stop solution. A detection method is also provided, wherein a P72 protein capture antibody, an African swine fever virus (inactivated), and a P72 protein monoclonal antibody labeled with a palladium-gold nanodendrite are sequentially constructed on a 96-well plate, and then a chromogenic substrate OPD and H2O2 are added. The palladium-gold nanodendrite particle mimics the efficient peroxidase-like performance of the enzyme material to generate an oxidized o-phenylenediamine (oxidized-OPD) with absorption and fluorescence emission, thereby achieving quantitative detection of the African swine fever virus (inactivated).