Reagent kit for LgG immunoblotting diagnosis of SARS coronavirus antibody

A diagnostic kit, coronavirus technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as inappropriate rapid detection of diseases, incorrect test results, complicated experimental techniques, etc., to achieve suitable mass production, high sensitivity, Ease of use
CN1453590AInactive Publication Date: 2003-11-05GUANGZHOU WONDFO BIOTECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGZHOU WONDFO BIOTECH
Publication Date
2003-11-05
Estimated Expiration
Not applicable · inactive patent
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Abstract

The present invention relates reagent kit for IgG immunoblotting diagnosis of SARS coronavirus antibody. The reagent kit consists of solid film, antiantibody of enzyme label and corresponding chromogenic substrate. The solid film is obtained through purifying SARS coronary totivirus lysate liquid antibody or recombining mixed antigen, gel electrophoresis to separate and transferring to film. The antiantibody of enzyme label is compounded with rabbet's antihuman IgG labeled with horseradish peroxidase or sheep's antihuman IgG labeled with alkaline phosphatase, 0.01 % concentration thimerosal as protecting agent, ox serum protein in 1 wt% and PBS in pH 7.4 in 0.01 M. The present invention may be used in detecting several kinds of antigen and antibody in clinical test department and sanitary epidemic prevention department.
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Description

technical field

[0001] The invention relates to a kit for detecting human anti-SARS coronavirus antibody (IgG) by immunoblotting method, and carrying out epidemiological investigation and clinical diagnosis of atypical pneumonia. Background technique

[0002] Atypical pneumonia (also known as Severe Acute Respiratory Syndrome, Severe Acute Respiratory Syndrome, abbreviated as SARS) has been raging around the world for nearly five months. On April 16, the World Health Organization officially announced a new type of coronavirus that has never been found in humans before. It is the pathogen of SARS. Coronavirus (Corona Virus) was discovered by Almeida et al. in 1968. It was named "coronavirus" because of its shape. Under the electron microscope, the virus envelope has spikes similar to the corona. Coronaviruses are widely classified and can infect humans, poultry and livestock, and can cause poultry infectious bronchitis, mouse hepatitis, porcine encephalomyelitis, feline infe...

Claims

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