The invention discloses a method for joint detection of PCT, CRP and IL-6 and a reagent kit for the method. The reagent kit comprises a first enveloping film and a second enveloping film. One end of the first enveloping film is connected with one end of the second enveloping film, and a labeled antibody mixture is enveloped in at least one area in the first enveloping film and comprises a first labeled antibody, a second labeled antibody and a third labeled antibody. The second enveloping film comprises a first area, a second area, a third area and a fourth area which are separated, wherein a first enveloped antibody, a second enveloped antibody and a third enveloped antibody are enveloped in the first area, the second area and the third area respectively, the first enveloped antibody and the first labeled antibody can be in specific binding with a first antigen, the second enveloped antibody and the second labeled antibody can be in specific binding with a second antigen, the third enveloped antibody and the third labeled antibody can be in specific binding with a third antigen, an antiantibody is enveloped in the fourth area, and the antiantibody can be in specific binding with the first labeled antibody, the second labeled antibody and the third labeled antibody. By means of the reagent kit, PCT, CRP and IL-6 can be quickly and accurately detected at the same time.