55 results about "Peroxiredoxin" patented technology

A brain damage-related disorder is diagnosed in a subject by detecting at least one polypeptide, or a variant or mutant thereof, selected from A-FABP, E-FABP, PGP 9.5, GFAP, Prostaglandin D synthase, Neuromodulin, Neurofilament L, Calcyphosine, RNA binding regulatory subunit, Ubiquitin fusion degradation protein 1 homolog, Nucleoside diphosphate kinase A, Glutathione S tranferase P, Cathepsin D, DJ-1 protein, Peroxiredoxin 5 and Peptidyl-prolyl cis-trans isomerase A (Cyclophilin A) in a sample of body fluid taken from the subject.

The invention discloses a sewage treatment agent special for printing and dyeing wastewater. The sewage treatment agent is prepared from, by weight, coal ash, wooden activated carbon, poly aluminium chloride, polyferric sulfate, polyacrylamide, ammonium persulfate, sodium carbonate, poly-ferric aluminum sulfate, aluminum sulfate, yttrium oxalate, manganese sulfate, sodium bentonite, zeolite, sepiolite, modified lignin sulfonate, alkylphenol ethoxylates, amino trimethylene phosphonic acid, menthone, thiourea, cationoid starch derivatives, sodium dodecyl benzene sulfonate, dimethyl diallyl ammonium chloride, octaphenyl polyoxyethyiene, glycerin monostearate, peroxiredoxin and microbial bacterial liquid. The microbial bacterial liquid is prepared from saccharomycetes, fusarium oxysporum, proteusbacillus vulgaris, neurospora and mycoplana. The invention further provides a preparation method of the sewage treatment agent. The prepared sewage treatment agent is good in treatment effect on the printing and dyeing wastewater, the preparation method is simple, and no secondary pollution exists.

This invention discloses an associating determination and reagent about high and low-density lipoprotein cholesterol. It utilizes affinity difference between lipoprotein and surface activator, adds agent one and agent two, and with the action of the assembling ionic surface activator, the CM, VLDL and LDL-C or HDL-C in the serum form the soluble complex, the extricate cholesterol generates H#-[2]O#-[2] with the catalytic reaction of cholesterol esterase (CHER) and cholesterol oxidase (CHO), and with the action of peroxydase (POD), the H#-[2]O#-[2] is cleared, and adds agent three, as with the action of a specific selective surface activator, only HDL-C or LDL-C granule is soluble, so with action of Trinder one can determine the content of HDL-C and LDL-C.

The present invention relates reagent kit for IgG immunoblotting diagnosis of SARS coronavirus antibody. The reagent kit consists of solid film, antiantibody of enzyme label and corresponding chromogenic substrate. The solid film is obtained through purifying SARS coronary totivirus lysate liquid antibody or recombining mixed antigen, gel electrophoresis to separate and transferring to film. The antiantibody of enzyme label is compounded with rabbet's antihuman IgG labeled with horseradish peroxidase or sheep's antihuman IgG labeled with alkaline phosphatase, 0.01 % concentration thimerosal as protecting agent, ox serum protein in 1 wt% and PBS in pH 7.4 in 0.01 M. The present invention may be used in detecting several kinds of antigen and antibody in clinical test department and sanitary epidemic prevention department.

A highly safe, inexpensive and widely utilizable method, which has a mechanism backed by scientific grounds, for improving an activity of ROS (reactive oxygen species)-scavenging enzyme group; An increase in the amount of an enzyme or promotion of an enzyme activity of the ROS scavenging enzyme group such as superoxide dismutase, catalase or peroxidase is caused in an organism having the ROS-scavenging enzyme with one or two or more kinds of substances selected from the group consisting of erythritol, mannitol, sorbitol and xylitol in 0.01-10% administration concentration.

The invention relates to a new fluoroimmunoassay method for human thymidine kinase fluoroimmunoassay based on magnetic nanometer grains. The invention sets up a quick and simple immunological detecting technology for the high flux human thymidine kinase (hTK1), and the technology is used for the hTK1 clinical examination. The hTK1 is fixed with covalence on the surface of an amido silanization superparamagnetism nanometer grain immobilized carrier through glutaric dialdehyde, a competitive immunoassay method is adopted, horse radish peroxidase is used as an enzyme labeling, ethyl-para-hydroxyphenyl acid is used as a fluorogenic substrate, thereby realizing the detecting for the hTK1. The amido silanization nucleocapsid-shaped magnetic nanometer grains are evenly dispersed in liquidoid, thereby having the advantages of fixation and uniformity, high specificity, good repeatability, quick reaction velocity, etc., under the action of an adscititious magnetic field, the invention can effectively realize the separation and enrichment of the immune complex and the reaction liquid of the nucleocapsid-shaped magnetic nanometer grains, the operation of the analytical method is simple, accurate, sensitive and fast, automatic detection is easy to be realized, and large batch of test task can be completed.

A heavy metal sewage treatment agent, consisting of the following substances in parts by mass: 21 parts of menthone, 25 parts of propylene glycol, 5 parts of acrylamide, 5 parts of sodium sulfoxylate, 3 parts of sodium acetate, 10 parts of glyceryl monostearate, 11 parts of tourmaline powder, 5 parts of starch, 20 parts of butane, 5 parts of dimethyl diallyl ammonium chloride, 10 parts of silicon dioxide, 20 parts of zeolite minerals, 5 parts of powdered activated carbon, 5 parts of sodium silicate, 12 parts of diatomaceous earth, 5 parts of potassium persulfate, 5 parts of dibenzoyl peroxide, 15 parts of peroxidoreductase, 12 parts of basic aluminum chloride, 8 parts of polyacrylamide, 30 parts of polymeric ferric sulfate, polymeric chlorine 18 parts of aluminum chloride, 2 parts of polysorbate, 20 parts of polyimide, and 1 part of polyethylene glycol.

The invention relates to a new ELISA test kit of human TFF3, and the key technique is characterized in that the specific anti-TFF3 antibody is prepared by a gene engineering method, an elisa plate coated by the antibody is used as an important part of the kit, and then is assembled with TFF3 multi-antibody, secondary antibody marked by horse radish peroxidase, TFF3 standard, TFF3 positive control, sample diluent, washing liquor, coloration solution and stopping solution into the ELISA test kit of human TFF3; ELISA is carried out by a bi-anti sandwich method to establish a measurement method which can sensitively and rapidly capture TFF3 concentration in human serum or cell and tissue culture, and has the characteristics of high specificity and high stability. The kit is mainly used for clinical research use such as general laboratory or gastrointestinal tract pathology.

The invention discloses a vascular endothelial growth factor (VEGF) receptor enzyme linked diagnosis kit. A VEGF receptor coated enzyme linked reaction plate, an enzyme conjugate prepared through a horse radish peroxidase (HRP)-marked monoclonal antibody and a protecting agent, VEGF protein freeze-dried powder serving as positive comparison, protein freeze-dried powder serving as negative comparison, sample diluting liquid, PBST concentrated washing liquid, a chromogenic substrate prepared through 3,3',5,5'-tetramethyl benzidine, 2MH2S04 serving as stop buffer and shrouding adhesive paper are arranged in a kit body. The kit can be used for diagnosing and prognosis of diseases related to VEGF receptors such as cancer, angiopathy, diabetes retina disease and rheumatoid arthritis, has the advantages of being accurate, special, flexible, stable, convenient and the like, and has wide application prospects.

The present invention relates to protein markers of gestational diabetes mellitus in urine and the use in early diagnosis. In particular, the present invention relates to the use of an identification reagent selected from the following proteins for preparing reagents for early diagnosis of gestational diabetes mellitus: protein NOV homolog, Peroxiredoxin-5 (PRDX-5), Hepatocyte growth factor-like protein (HFGL) and the combination thereof. The protein NOV homolog, the Peroxiredoxin-5 and the Hepatocyte growth factor-like protein and the combination thereof have good clinical application prospects for early diagnosis of gestational diabetes mellitus.

Specific plant extracts are applied topically to upregulate genes which code for proteins to prevent the build-up of and mitigate the activity of various harmful materials within the skin. These upregulated proteins include glutathione transferases, peroxiredoxins, oxidoreductases, glutaredoxins and glutathione syntheses. These proteins produce additional glutathione, glyoxalase I, maintain cellular redox homeostasis, reduce hydrogen peroxide and alkylhydroperoxides, and conjugate glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Keratinocytes have been induced to significantly upregulate glutathione and glyoxalase I protein expression. Additionally, keratinocytes pre-treated with diallyl trisulfide have increased viability when exposed to ozone, UV radiation or cigarette smoke extract. The active material can be extracted from plants such as garlic, onions, shallots, leeks, chives, scallions, brussel sprouts, broccoli, cabbage, cauliflower, bok choy, kale, mustard, turnip or radish, may include any compound of the allyl sulfide family, and may be of synthetic origin.

The invention discloses a method for adopting enzyme immune technology to test hepatitis C virus antibody, it also discloses the hepatitis C virus multiple bit table jogging antibody and the enzyme label connecting arm. The testing method comprises: preparing the hepatitis C virus multiple bit table jogging antibody, preparing the jogging antibody and the label connecting arm, preparing the jogging antibody label horse radish peroxides compound, using the jogging antibody to uterus the enzyme checker board, adding tested antibody and enzyme label antibody compound and so on.

The invention provides application of peroxidase Prdx1 (peroxiredoxin-1) to preparation of a drug for preventing and treating cerebral injury diseases based on cerebral arterial thrombosis, vascular dementia and other cerebrovascular diseases. According to the invention, a Prdx1 gene-carrying overexpression plasmid and a recombinant lentiviral vector are established, cell and whole animal level tests prove that the Prdx1 is capable of effectively reversing the ischemic injury of vascular endothelial cells, protecting a BBB (Blood Brain Barrier) and relieving nerve function deficits caused by cerebral microcirculatory disturbance, and therefore, the effect of preventing and treating the cerebrovascular diseases is achieved. The Prdx1 gene lentiviral vector provided by the invention is expected to become an important way for developing the drug for treating the cerebrovascular diseases, and experimental basis is provided to the application of the Prdx1 gene to gene therapy of the cerebrovascular diseases.

The invention relates to an enzyme-linked immunosorbent assay (ELISA) kit and a preparation method thereof, and more specifically relates to a novel anti-human FN1 protein monoclonal antibody coated ELISA plate and a preparation method of the ELISA kit. A key step of the preparation method is that: the specific mouse anti-human FN1 protein monoclonal antibody is prepared by genetic engineering method, and is purified; and then the ELISA plate is coated with the specific mouse anti-human FN1 protein monoclonal antibody, and is used as a constituent part of the ELISA kit. The ELISA kit also comprises FN1 recombined protein standard, polyclonal antibody of anti-human FN1, horse radish peroxidase-labeled second antibody, a sample diluent, a washing liquid, an antibody diluent, a colour reagent, and a stop solution. The ELISA kit can be used for rapid and convenient detection of FN1 concentration of human serum and blood plasma, and is mainly provided for scientific research or clinical research of cancer related diseases.

A hepatitis B virus gene grouping method by retroactive point hybridizing technology includes: 1) taking X zone sequence of HBV gene group as main designing grouping primmer and probe; 2) fixing active amino-marked probe on nylon membrane to obtain detecting membranous strip; 3) HBV DNA amplifying marked biotin of primmer; 4) amplified product hybridizing with detecting membranous strip, and color developing POD and TMB; 5) discriminating gene grouping end. It achieves low cost and simple operation.

A composition for removal of biofilm in the ears is useful for the treatment of ear infections such as otitis media, particularly those infections caused by Pseudomonas aeruginosa. In general, the composition comprises: (1) a quantity of at least one enzyme that catalyzes the hydrolysis of a bond that connects two monosaccharides in a polysaccharide or that connects a monosaccharide with a protein molecule in a glycoprotein sufficient to break down biofilm in the ear; and (2) a pharmaceutically acceptable carrier suitable for administration into the ear canal. The composition can further include ingredients such as a steroid, lysozyme, lactoferrin, or a peroxidase; if a peroxidase is included, the composition can further include an oxidase to generate peroxide as well as a substrate for the oxidase. The composition can be used in methods for treatment of an ear infection based on the ability of the composition to dissolve biofilm in the ear.

Bertrand(1982) hydrolytic generates trophicardyl from trophicardyl acid by nucleotidase, and gains H2O2 through the reaction of the enzyme1 and enzyme2. Use of H2O2 from Trinder's reaction to combine hydrogen provider3,5-double CL-2-hydroxide benzene sulphur acid and 4- to create a amaranth in the situation of the catalase. By watching the velocity of increase of the degree of the amaranth absorbing light at 510 nms to gain 5' nucleotidase activity. The invention uses the aniline compound ADOS, ADPS, ALPS, TODB, TOOS and TOPS as hydrogen provider for Trinder's reaction and increases the reaction ability. The invention also involves a reagent box used for implementing the method mentioned above.

The invention relates to the field of biotechnology and medical detection, and aims at providing a saliva-anti-mitochondrial-antibody-M2-type enzyme-linked immunity detection kit. The kit is prepared from an AMA-M2 standard substance, a polystyrene ELISA micro-pore plate coated with an M2 antigen, a horseradish peroxidase-conjugated antibody, a sample diluent and auxiliary reagent, wherein the enzyme-labeled second antibody is anti-human IgG labeled by horseradish peroxidase; the sample diluent is a BSA-PBS solution with the mass concentration of 0.1%; the auxiliary reagent comprises substrate color developing liquid, reaction stopping liquid and washing damping liquid. The kit is easier and more convenient to use, the expression level of AMA-M2 in saliva of a physical examination person can be directly detected, a wound-free detection mean is achieved, and the defects exist in blood path detection are overcome. The saliva-anti-mitochondrial-antibody-M2-type enzyme-linked immunity detection kit can be more safely, conveniently and rapidly applied to screening of PBC and assisting diagnosis of PBC, and data supporting is provided for treatment of later disease and improving prognosises of a patient.

The invention discloses a pigeon Newcastle disease virus monoclonal antibody, and applications thereof in preparation of a diagnosis and detection kit. The pigeon Newcastle disease virus monoclonal antibody is high in sensitivity, specificity, and titer. The colloidal gold diagnostic kit is used for detecting whether the liver, spleen, lung, brain, or heat tissue homogenate of infected pigeons orpigeons newly died of illness, and oral and nasal cavity secretion contains pigeon Newcastle disease virus, so that auxiliary diagnosis of pigeon Newcastle disease is realized. Response is rapid; thewhole operation time is only 15min; no professional is required; popularization is convenient. The competitive ELISA kit used for detecting the pigeon Newcastle disease virus antibody is capable of representing the titer of the pigeon Newcastle disease virus antibody in serum through detecting the inhibition effect of serum antibody in a detected sample on horseradish peroxidase labelled anti-pigeon Newcastle disease virus monoclonal antibody combined pigeon Newcastle disease virus antigen, operation of the kit is simple and reliable.

The invention provides a kit for detecting an anti-peroxiredoxin-1-IgG antibody. The kit consists of antigen protein peroxiredoxin-1, a solid-phase carrier, a labeled antibody, an antigen diluent, a sample dilution buffer solution, an antibody diluent, a substrate color developing agent, a washing solution, a standard substance, a positive quality control substance and a negative quality control substance. According to the kit disclosed by the invention, the anti-peroxiredoxin-1-IgG antibody in serum to be detected is detected by utilizing an indirect method reaction principle in combination with magnetic particle chemiluminescence immunoassay. According to the invention, the autoantibody aiming at the target antigen peroxiredoxin-1 in the serum of the patient with the autoimmune nephrotic syndrome is identified for the first time; the kit provided by the invention provides a basis for molecular mechanism research and clinical diagnosis and treatment of autoimmune nephrotic syndromes related to peroxiredoxin-1 and peroxiredoxin-1-IgG autoantibodies at home and abroad.

The invention discloses a cDNA of a trachinotus ovatus peroxiredoxin gene Prx1. The nucleotide of the cDNA is as shown in the SEQ ID NO.1 in the description. The invention further discloses an expression vector containing the trachinotus ovatus peroxiredoxin gene, a recombinant microorganism transformed by utilizing the vector, and a method for preparing the trachinotus ovatus peroxiredoxin gene by utilizing the microorganism. By adopting a genetic engineering technology, a recombinant trachinotus ovatus peroxiredoxin protein can be prepared.

The present invention relates to a process for extracting beneficial compounds, in particular growth factors, such as TGF beta and IGF-1 from milk. In this process a hydrophobic interaction chromatography step is included. A resin having a butyl group, or a phenyl group as the ligand is used as hydrophobic interaction resin. The resin can be eluted with a salt gradient which, when the ligand is a phenyl group, contains substantially no alcohol, and thus resulting in fractions enriched in the desired growth factors. These fractions can be separated further by means of a hydroxyapatite column.

The invention provides a kit for quantitatively detecting contents of enrofloxacin in food and the application thereof; the kit comprises reference materials, horse radish peroxidase (HRP) marked enrofloxacin antigen, enrofloxacin specific antibody, nano magnetic particle separating agents, luminescent substrate solution and lotion. The inventive kit has the advantages of easy storage, and highly stable, sensitive and precise detecting method.

The invention relates to a new fluoroimmunoassay method for human thymidine kinase fluoroimmunoassay based on magnetic nanometer grains. The invention sets up a quick and simple immunological detecting technology for the high flux human thymidine kinase (hTK1), and the technology is used for the hTK1 clinical examination. The hTK1 is fixed with covalence on the surface of an amido silanization superparamagnetism nanometer grain immobilized carrier through glutaric dialdehyde, a competitive immunoassay method is adopted, horse radish peroxidase is used as an enzyme labeling, ethyl-para-hydroxyphenyl acid is used as a fluorogenic substrate, thereby realizing the detecting for the hTK1. The amido silanization nucleocapsid-shaped magnetic nanometer grains are evenly dispersed in liquidoid, thereby having the advantages of fixation and uniformity, high specificity, good repeatability, quick reaction velocity, etc., under the action of an adscititious magnetic field, the invention can effectively realize the separation and enrichment of the immune complex and the reaction liquid of the nucleocapsid-shaped magnetic nanometer grains, the operation of the analytical method is simple, accurate, sensitive and fast, automatic detection is easy to be realized, and large batch of test task can be completed.

A brain damage-related disorder is diagnosed in a subject by detecting at least one polypeptide, or a variant or mutant thereof, selected from A-FABP, E-FABP, PGP 9.5, GFAP, Prostaglandin D synthase, Neuromodulin, Neurofilament L, Calcyphosine, RNA binding regulatory subunit, Ubiquitin fusion degradation protein 1 homolog, Nucleoside diphosphate kinase A, Glutathione S tranferase P, Cathepsin D, DJ-1 protein, Peroxiredoxin 5 and Peptidyl-prolyl cis-trans isomerase A (Cyclophilin A) in a sample of body fluid taken from the subject.

The invention provides a preparation method and an application of a co-immobilized enzyme electrode which is used for detecting p-dihydroxybenzene and o-dihydroxybenzene. The preparation method comprises steps of: immobilizing tyrosinase and peroxidase on oxidized grephene to obtain a co-immobilized enzyme liquid and smearing dropwisely the co-immobilized enzyme liquid and a chitosan solution successively on a surface of a glassy carbon electrode to obtain the co-immobilized enzyme electrode which is used for detecting p-dihydroxybenzene and o-dihydroxybenzene. The electrode is low in cost, is easy and convenient to operate, is quick and sensitive, is low in detection limit, is good in selectivity and can be used for detecting microscale p-dihydroxybenzene and o-dihydroxybenzene in environment.

A method for preparing labeled antibody of rabbit resisting goose IgY+IgYú¿í¸Fcú®ú¿H+Lú®horseradish perxidase includes applying protein purification technique and enzyme-label technique of salting-out and chromatography ,immunizing rabbit for purifying and preparing rabbit resisting goose IgY+IgYú¿í¸Fcú®ú¿H+Lú®antibody based on purification of yolk immune globulin ,combining said antibody with horseradish peroxidase through modified sodium periodate ,utilizing the salting-out and sephodex G200 purification for obtaining antibody product .

The invention discloses a novel method for preparing an enzyme-labeled antibody. Some unavoidable problems, particularly the tedious preparation of the enzyme-labeled antibody based on covalent binding usually exist in an enzyme-linked immunosorbent assay method. Therefore, the antibody is bound with a metal organic framework (MOF) with catalytic activity to peroxidase so as to form a bidirectional MOF / antibody composite material, and the bidirectional MOF / antibody composite material can be applied to a colorimetric immunoassay method. The MOF does not influence the capture capacity of the antibody to the antigen, and the antibody is not influenced by high temperature and biological degradation, so that the stability of the antibody is improved. More importantly, the signal amplification of the MOF with catalytic activity to peroxidase can be realized in a colorimetric immunoassay experiment, so that the detection sensitivity is improved. The method has the advantages of simplicity andconvenience in operation, low cost, high efficiency and the like.