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Joint determination method and reagent for high-low density lipoprotein cholesterol

A method for measuring lipoproteins, which is applied in biological testing, material inspection products, etc., can solve problems such as immature technology, affecting measurement results, and high prices, and achieve the effect of reducing costs and being simple and fast to operate

Inactive Publication Date: 2005-01-26
王贤俊
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Judging from the current domestic direct method detection reagents, there are still technical immaturity problems. For example, the measurement reagent disclosed by the Chinese Patent Publication No. CN-1281981A is easy to form flocculation with some polyanions because of the high concentration of some metal ions in its components. substances that affect the measurement results
Another example is the assay reagent of Chinese Patent Publication No. CN-1379234A. Mg2+ is relatively high in its composition, which not only can inhibit the activity of various enzymes in the reagent, but also easily forms Mg(OH) in a buffer system with an alkaline pH value. Precipitation affects the determination
Due to the existence of the above technical problems, the domestic direct method for the determination of high and low density lipoproteins cannot be widely used.
Imported reagents are relatively mature in technology, but the price is relatively high, and some basic hospitals in China cannot carry out

Method used

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  • Joint determination method and reagent for high-low density lipoprotein cholesterol
  • Joint determination method and reagent for high-low density lipoprotein cholesterol
  • Joint determination method and reagent for high-low density lipoprotein cholesterol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Reagent 1:

[0051] pH value 7.5HEPES-NaoH buffer solution 50mmol / L

[0052] Polyoxyethylene alkylene phenyl ether 4g / L

[0053] Cholesterol esterase 4KU / L

[0054] Cholesterol oxidase 2KU / L

[0055] Peroxidase 8KU / L

[0056] Ascorbate oxidase 1KU / L

[0057] Polyethylene glycol 8000 30g / L

[0058] TOOS 4mmol / L

[0059] Potassium ferrocyanide 0.002mmol / L

[0060] Sodium cholate 1mmol / L

[0061] Bovine serum albumin 2mmol / L

[0062] ProClin4000.5g / L

[0063] Reagent 2:

[0064] pH value 7.5HEPES-NaoH buffer solution 50mmol / L

[0065] Polyoxyethylene alkylene phenyl ether 4g / L

[0066] Cholesterol esterase 4KU / L

[0067] Cholesterol oxidase 2KU / L

[0068] Peroxidase 8KU / L

[0069] Ascorbate oxidase 1KU / L

[0070] Saponin 0.2mmol / L

[0071] TOOS 4mmol / L

[0072] Potassium ferrocyanide 0.002mmol / L

[0073] Sodium cholate 1mmol...

Embodiment 2

[0094] The high-low-density lipoprotein cholesterol joint detection reagent used in this embodiment is that cholesterol esterase 4KU / L and cholesterol oxidase 2KU / L in embodiment 1 are changed to the following concentrations and combinations, and the same measurement as in embodiment 1 is carried out , the results are shown in Table 2 and image 3 , Figure 4 shown.

[0095] Cholesterol esterase 2KU / L

[0096] Cholesterol oxidase 1KU / L

[0097] Table 2

[0098]

[0099] 13

[0100] From the above results, it can be seen that substantially the same results can be obtained even when cholesterol esterase and cholesterol oxidase are reduced by 1 / 2 compared with Example 1.

Embodiment 3

[0102] The high-low-density lipoprotein cholesterol joint detection reagent used in the present embodiment is to change the HEPES-NaoH buffer solution with a pH value of 7.5 from 50mmol / L to 20mmol / L in embodiment 1, and carry out the same measurement of embodiment 1, The results are shown in Table 3 and Figure 5 , Figure 6 shown.

[0103] table 3

[0104]

[0105] 2

[0106] from Table 3 and Figure 5 , Figure 6 It can be seen that compared with Example 1, the HEPES-NaoH buffer solution with a pH value of 7.5 is reduced by 1 / 2, and approximately the same results can be obtained, so the cost of the assay can be reduced.

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Abstract

This invention discloses an associating determination and reagent about high and low-density lipoprotein cholesterol. It utilizes affinity difference between lipoprotein and surface activator, adds agent one and agent two, and with the action of the assembling ionic surface activator, the CM, VLDL and LDL-C or HDL-C in the serum form the soluble complex, the extricate cholesterol generates H#-[2]O#-[2] with the catalytic reaction of cholesterol esterase (CHER) and cholesterol oxidase (CHO), and with the action of peroxydase (POD), the H#-[2]O#-[2] is cleared, and adds agent three, as with the action of a specific selective surface activator, only HDL-C or LDL-C granule is soluble, so with action of Trinder one can determine the content of HDL-C and LDL-C.

Description

technical field [0001] The invention relates to a method and reagents for joint determination of high and low density lipoprotein cholesterol. Background technique [0002] Many epidemiological and clinical studies have confirmed that the occurrence of atherosclerosis (AS) and coronary heart disease (CHD) is negatively correlated with the level of high-density lipoprotein cholesterol (HDL-C) in serum, while it is negatively correlated with the level of low-density lipoprotein cholesterol (LDL-C). -C) levels are positively correlated. Clinically, the ratio of high-density lipoprotein cholesterol to low-density lipoprotein cholesterol is commonly used to indicate the incidence tendency of coronary heart disease, which is called coronary heart disease index. The most valuable index of risk factors for cardiovascular and cerebrovascular diseases in blood lipid determination. [0003] At present, although there are many methods for measuring HDL-C and LDL-C, such as ultracentrif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N33/92
Inventor 王贤俊
Owner 王贤俊
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