Joint determination method and reagent for high-low density lipoprotein cholesterol
A method for measuring lipoproteins, which is applied in biological testing, material inspection products, etc., can solve problems such as immature technology, affecting measurement results, and high prices, and achieve the effect of reducing costs and being simple and fast to operate
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Embodiment 1
[0050] Reagent 1:
[0051] pH value 7.5HEPES-NaoH buffer solution 50mmol / L
[0052] Polyoxyethylene alkylene phenyl ether 4g / L
[0053] Cholesterol esterase 4KU / L
[0054] Cholesterol oxidase 2KU / L
[0055] Peroxidase 8KU / L
[0056] Ascorbate oxidase 1KU / L
[0057] Polyethylene glycol 8000 30g / L
[0058] TOOS 4mmol / L
[0059] Potassium ferrocyanide 0.002mmol / L
[0060] Sodium cholate 1mmol / L
[0061] Bovine serum albumin 2mmol / L
[0062] ProClin4000.5g / L
[0063] Reagent 2:
[0064] pH value 7.5HEPES-NaoH buffer solution 50mmol / L
[0065] Polyoxyethylene alkylene phenyl ether 4g / L
[0066] Cholesterol esterase 4KU / L
[0067] Cholesterol oxidase 2KU / L
[0068] Peroxidase 8KU / L
[0069] Ascorbate oxidase 1KU / L
[0070] Saponin 0.2mmol / L
[0071] TOOS 4mmol / L
[0072] Potassium ferrocyanide 0.002mmol / L
[0073] Sodium cholate 1mmol...
Embodiment 2
[0094] The high-low-density lipoprotein cholesterol joint detection reagent used in this embodiment is that cholesterol esterase 4KU / L and cholesterol oxidase 2KU / L in embodiment 1 are changed to the following concentrations and combinations, and the same measurement as in embodiment 1 is carried out , the results are shown in Table 2 and image 3 , Figure 4 shown.
[0095] Cholesterol esterase 2KU / L
[0096] Cholesterol oxidase 1KU / L
[0097] Table 2
[0098]
[0099] 13
[0100] From the above results, it can be seen that substantially the same results can be obtained even when cholesterol esterase and cholesterol oxidase are reduced by 1 / 2 compared with Example 1.
Embodiment 3
[0102] The high-low-density lipoprotein cholesterol joint detection reagent used in the present embodiment is to change the HEPES-NaoH buffer solution with a pH value of 7.5 from 50mmol / L to 20mmol / L in embodiment 1, and carry out the same measurement of embodiment 1, The results are shown in Table 3 and Figure 5 , Figure 6 shown.
[0103] table 3
[0104]
[0105] 2
[0106] from Table 3 and Figure 5 , Figure 6 It can be seen that compared with Example 1, the HEPES-NaoH buffer solution with a pH value of 7.5 is reduced by 1 / 2, and approximately the same results can be obtained, so the cost of the assay can be reduced.
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