29 results about "Cholesterol Esterase" patented technology

The present disclosure provides polypeptides having a cholesterol-lowering function in a soybean protein hydrolysate, a preparation method thereof, and uses thereof. The polypeptides are derived from peptide fragments having a molecular docking “binding energy” value of less than −1.2 kcal with cholesterol esterase. The peptide fragments of the polypeptides that are subjected to molecular docking with cholesterol esterase are derived from 23 polypeptides having a Peptide Ranker bioactivity score of greater than 0.9. The polypeptides possess a significant blood-lipid-lowering function.

According to the invention, the traditional fermented koumiss in Xinjiang is collected, two novel multifunctional probiotic strains, namely, the lactobacillus plantarum SR6 GDMCC NO.66817 strain and the enterococcus faecalis SM5 GDMCC NO.66816 strain with good compatibility are separated and screened out, and the two strains have the characteristics of reducing blood fat, reducing cholesterol, removing triglyceride, resisting oxidation and antagonizing pathogenic bacteria, and have the characteristics of good cholate and gastrointestinal tract tolerance, high survival rate and good safety. The growth characteristics and compatibility of the two strains are improved; the inhibition rate of cholesterol esterase and pancreatic lipase; the method comprises the following steps: evaluating the triglyceride removal rate, antioxidant activity, pathogenic bacterium resistance, artificially simulated gastrointestinal fluid tolerance, strain drug resistance and safety, compounding lactobacillus plantarum SR6 and enterococcus faecalis SM5 strains according to a ratio of 1: 1 to prepare a freeze-dried bacterial agent, and keeping good survival rate (pgt; 0.05) and functional characteristics, the strain can be used as a functional direct vat set starter and is directly applied to production of fermented dairy products, and the biological activity of food is improved.

The application provides a recombinant cholesteryl esterase, a polynucleotide thereof, a recombinant plasmid, an expression system and application. The application belongs to the technical field of protein engineering, and specifically, the amino acid sequence of the recombinant cholesteryl esterase provided by the application is shown as SEQ ID No. 1, and the nucleotide sequence for encoding the recombinant cholesteryl esterase is shown as SEQ ID No. 2. The sequence of the recombinant cholesteryl esterase provided by the application has a full length of 482 amino acids, is derived from Aspergillus ruber, has the advantage of high thermal stability, and has a thermal stability deviation of less than 10% after thermal storage at 37 DEG C for 11 days and after 30 days of on-board opening, and has a wide application prospect in the field of in-vitro diagnostic reagents.

The invention belongs to the technical field of hippophae rhamnoides fruit residue utilization, and particularly relates to application of hippophae rhamnoides fruit residue extract in preparation of a medicine for treating diabetic complications. Experiments find that the sea buckthorn pomace extract has a high inhibition rate on cholesterol esterase and alpha-amylase, and the sea buckthorn pomace extract mainly contains malvacic acid, aspartic acid, luteolin and vernonic acid, and key targets mainly include IL6, AKT1, TNF and PPARG, and the sea buckthorn pomace extract has a high inhibition rate on cholesterol esterase and alpha-amylase. The fructus hippophae pomace extract plays a role in regulating blood fat regulation, atherosclerosis, HIF-1 signal transduction, AGE-RAGE signal transduction and other pathways in diabetic complications.

The application belongs to the technical field of biological processing, and specifically discloses a sea cucumber flower polypeptide with lipid-lowering efficacy as well as a preparation method and application thereof. The sea cucumber flower polypeptide with lipid-lowering efficacy is composed of short peptides with a molecular weight less than 1000 Da; the short peptides are one or more of GTGATGTF, FTGIVGSL, LAIGETEF, VGITDIESF, VDDEF and YDDVP. The application discloses a sea cucumber flower polypeptide with lipid-lowering efficacy as well as a preparation method and application thereof. The sea cucumber flower polypeptide has a significantly improved inhibition rate on cholesteryl esterase and pancreatic lipase, and has a stronger adsorption capacity on bile acid. The sea cucumber flower polypeptide does not contain the enzyme cutting sites of pepsin and trypsin, can resist secondary digestion and degradation after being taken into the human body, stably maintains structural integrity and biological activity, and solves the problem that traditional lipid-lowering peptides are easily destroyed in activity.

The invention discloses a compound enzyme solution for improving total cholesterol test current, which is prepared from the following components in percentage by weight: 20 to 200mM of buffer solution, 0.5 to 200mg / mL of cholesterol oxidase, 0.1 to 100mg / mL of cholesterol esterase, 0.1 to 25mg / mL of peroxidase, 1 to 6 percent of potassium ferricyanide, 0.1 to 8 percent of glucoside and the balance of water. And the cellulose accounts for 0.01%-2%. When a whole blood sample is tested, the compound enzyme liquid of lipoprotein can be quickly decomposed, so that the test current is improved, and the test time is shortened.

The invention belongs to the technical field of functional microorganism application, and particularly relates to a novel lipid-lowering peptide derived from a probiotic thallus and application thereof, the novel lipid-lowering peptide is a crushed substance derived from a probiotic lactobacillus plantarum thallus, and the molecular weight of the novel lipid-lowering peptide is less than 3kDa. And two key polypeptides APGFGDR (AP7) and TESGAFGR (TE8) are identified from the components, and can specifically inhibit the activities of pancreatic lipase and pancreatic cholesterol esterase. The extracted thallus peptide can be efficiently combined with cholate to inhibit the formation of cholesterol micelles, and has a remarkable inhibiting effect on pancreatic lipase and pancreatic cholesterol esterase. Animal experiments show that the mycopeptide can effectively improve lipid metabolism disorder induced by high fat diet, inhibit weight gain, reduce serum and liver lipid levels and relieve fatty degeneration and inflammation of the liver. The lipid-lowering peptide disclosed by the invention is high in safety and multi-target in action, and can be used for preparing functional products for preventing or treating metabolic diseases such as obesity, hyperlipidemia and the like.

The present invention relates to an electrochemical sensor for multiple detection, a preparation method thereof, and an application thereof, and belongs to the field of biological detection technology. The present invention provides an electrochemical sensor for multiple detection, wherein the reference electrode, the working electrode, and the counter electrode of the sensor are sequentially modified with a GOD-HRP-CS-MB layer, a LOD-HRP-FMN layer, and a COD-CEH-HRP-β-CD layer from bottom to top, wherein the components of the GOD-HRP-CS-MB layer include horseradish peroxidase, methylene blue, glucose oxidase, and chitosan, the components of the LOD-HRP-FMN layer include lactate oxidase and flavin mononucleotide, and the components of the COD-CEH-HRP-β-CD layer include β-cyclodextrin, ferrocene, cholesterol oxidase, horseradish peroxidase, and cholesterol esterase. The sensor can simultaneously measure the concentrations of glucose, lactate, and cholesterol in a sample to be tested.

A dry analytical reagent for total cholesterol detection comprises, from top to bottom, an upper supporting layer, a diffusion layer, a reagent layer, a support, and a lower supporting layer, wherein the diffusion layer, the reagent layer, and the support are sequentially arranged in a middle position between the upper supporting layer and the lower supporting layer, the pores at both ends of the upper supporting layer and the lower supporting layer are bonded by the middle supporting layer, and a sample addition hole and a test hole are respectively arranged in the middle position of the upper supporting layer and the lower supporting layer, the diffusion layer is an isotropic porous diffusion membrane with a porosity of 50-85%; the diffusion layer is composed of polystyrene microspheres, an adhesive, and a surfactant, and the particle size of the polystyrene microspheres is 0.1-1 μm; the reagent layer includes a surfactant, cholesterol esterase, cholesterol oxidase, horseradish peroxidase, a colorless dye, a buffer, a stabilizer, and a water-soluble high molecular polymer. The diffusion layer prepared by the present invention has a diffusion path of tens to hundreds of nanometers, which can quickly absorb, evenly distribute, measure, and quickly transmit multiple analytes.

The invention relates to the technical field of biochemistry, in particular to compound enzyme liquid, detection test paper, a detection system and a detection method for total cholesterol detection. The compound enzyme liquid for total cholesterol detection comprises cholesterol oxidase, cholesterol esterase, peroxidase, an electronic mediator and a surfactant composition, and the detection method for total cholesterol detection comprises the following steps: inserting detection test paper into a detection system, putting a to-be-detected sample close to a test paper sample adding port, and adding the to-be-detected sample into the test paper sample adding port; a to-be-detected sample is incubated with the compound enzyme liquid loaded on the electrode layer of the detection test paper, and the electrochemical response is determined by applying potential through a detection system. By using the detection method, the total cholesterol can be detected through an electrochemical method, the detection speed is high, the sensitivity is high, and the accuracy is high.

The present application relates to a kind of fish oil polypeptide composition for reducing blood fat and its application, belong to the field of biological medicine and functional food.The composition includes fish oil and pumpkin seed source active peptide C2-1 (molecular weight <3kDa), wherein active peptide accounts for 1-5% of the total weight of composition.Its preparation method includes: pumpkin seed protein complex zymolysis, ultrafiltration separation, anion exchange chromatography purification obtains C2-1 active peptide (KRGGRFL), then with fish oil homogenization into stable dispersion.The composition significantly reduces serum total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) by simultaneously inhibiting cholesterol esterase and lipase activity, and improves high-density lipoprotein cholesterol (HDL-C).Animal experiments show that its lipid-lowering effect is better than single fish oil, and is comparable to chemical drugs.The present application first realizes polypeptide-fish oil synergistic effect, provides safe and efficient new scheme for hyperlipidemia prevention and treatment.

The invention discloses selenium-enriched spirulina carbon dots as well as a preparation method and application thereof, and belongs to the technical field of medicines. The selenium-rich spirulina powder is obtained by taking selenium-rich spirulina growing to a logarithmic phase as a raw material, centrifuging, collecting and freeze-drying. Uniformly mixing the spirulina platensis with a catalyst and deionized water according to a mass ratio of 1: (0.1-0.8): 20, then putting the mixture into a high-temperature reaction kettle to react at 140-260 DEG C for 1.5-5.5 hours, dialyzing the reaction liquid for 24 hours after the reaction is finished, centrifuging, and freeze-drying the dialysate to obtain the selenium-enriched spirulina platensis carbon dots with the yield of 70-95%. The selenium-enriched spirulina carbon dots provided by the invention not only have the inhibition capability of alpha-amylase, alpha-glucosidase, cholesterol esterase and pancreatic lipase and the cholate binding capability, but also can improve the endogenous antioxidant defense capability of model organisms while removing exogenous oxidizing substances, so that the glycolipid metabolism balance is efficiently regulated and controlled.

This invention discloses a dry chemical reagent for total cholesterol detection, comprising a diffusion layer, a spacer layer, and a reagent layer sequentially overlapped. The diffusion layer includes reflective particles, a binder, a surfactant, cholesterol esterase, and cholesterol oxidase. The reagent layer includes a carrier, horseradish peroxidase, an indicator composition, and a buffer solution. The dry chemical detection reagent of this invention employs a coating method, layering a premixed homogeneous slurry onto a support layer. A spacer layer blocks interlayer interference, and phenolic substances replace photosensitizers as chromogens, combined with a specific ratio of substrate and buffer system. The multi-layered, spacer-free, low-interference vertical detection system constructed by this invention significantly improves the repeatability, accuracy, and stability of the detection reagent.

The invention belongs to the technical field of biological processing, and particularly discloses sea cucumber flower polypeptide with a lipid-lowering effect as well as a preparation method and application of the sea cucumber flower polypeptide. The sea cucumber flower polypeptide with the lipid-lowering effect is composed of oligopeptides with the molecular weight smaller than 1000 Da. And the oligopeptide is one or more of GTGATGTF, FTGIVGSL, LAIGETEF, VGITDIESF, VDDEF and YDDVP, and the oligopeptide is one or more of GTGATGTF, FTGIVGSL, LAIGETEF, VGITDIESF, VDDEF and YDDVP The invention discloses a sea cucumber flower polypeptide with a lipid-lowering effect and a preparation method and application thereof.The inhibition rate of the sea cucumber flower polypeptide on cholesterol esterase and pancreatic lipase is remarkably increased, the adsorption capacity of the sea cucumber flower polypeptide on bile acid is higher, and the sea cucumber flower polypeptide does not contain restriction enzyme cutting sites of pepsase and trypsin and is free of toxic and side effects. The lipid-lowering peptide can resist secondary digestion and degradation after human body ingestion, stably maintain structural integrity and biological activity, and solve the problem that the traditional lipid-lowering peptide is easy to digest and damage the activity.

The invention provides Porro lactobacillus mucus with a lipid-lowering function and application of the Porro lactobacillus mucus, and belongs to the technical field of microorganisms. The invention relates to a lactobacillus mucus strain FVT08 of Porro, and the preservation number of the lactobacillus mucus strain FVT08 is CGMCC (China General Microbiological Culture Collection Center) No.33640. The strain FVT08 not only has high lipid-lowering capacity under in-vitro conditions, can inhibit the activity of lipase (pancreatic lipase and cholesterol esterase) to reduce fat absorption of a body, but also has high bile salt hydrolase activity to promote cholesterol discharge, and at the animal level, the strain FVT08 colonizes in intestinal tracts to promote cholesterol discharge. The liver blood fat level and the liver tissue antioxidant level are reduced, lipid metabolism related gene expression and intestinal flora disorder are improved, and the content of short-chain fatty acids (propionic acid, n-butyric acid, isobutyric acid, n-valeric acid and isovaleric acid) can be increased; meanwhile, the Lactobacillus mucilaginosus FVT08 of Porro can obviously reduce the expression level of hepatic tissue inflammatory factors and improve hepatic tissue structure damage caused by obesity.

The invention relates to the technical field of medicines, and provides a triterpenoid compound in ganoderma leukemia as well as a preparation method and application of the triterpenoid compound. Adding pure methanol according to a solid-liquid ratio of 1: 10, extracting at 60 DEG C for 2h (hour, hour), filtering to obtain a methanol extracting solution, repeating for three times, and combining filtrates; concentrating the filtrate to 1 / 5 of the original volume at 50 DEG C, and adding distilled water with the volume fraction of 2 / 3 until the methanol concentration is 60% to obtain a preparation raw material; the preparation raw materials are subjected to coarse separation in a C18 column. The invention proves that the DM (12-O-Deacetuleuccontextin M) has antioxidant activity, can inhibit the activity of alpha-glucosidase, cholesterol esterase and pancreatic lipase, and shows a multi-target lipid-lowering potential. In a high glucose induced nematode model, the DM can be used for effectively reducing lipid accumulation and lowering the level of TG (triglyceride) and NEFA (non-esterified fatty acid), and the DM can be used for effectively reducing the level of TG (triglyceride) and NEFA (non-esterified fatty acid).

Functional minerals produced from plant ash offer new applications, including the promotion of lipase enzyme activity, the promotion of cholesterol esterase or cholesterol oxidase enzyme activity, and the promotion of micronization of oil droplets of oily substances. [Solution] A plant ash selected from wood ash, grass ash, and seaweed ash obtained by burning trees, grasses, and seaweeds respectively is used as a raw material. A mixed ash obtained by mixing seaweed ash, or 50% by weight or more of seaweed ash, with at least one of wood ash and grass ash, is reheated, and a mineral-containing lipase enzyme activity enhancer, cholesterol esterase or cholesterol oxidase enzyme activity enhancer, and oil droplet micronizer are obtained by extracting the resulting reheated ash with water.

The invention relates to a blood fat reducing fish oil polypeptide composition and application thereof, and belongs to the field of biological medicine and functional food. The composition comprises fish oil and pumpkin seed active peptide C2-1 (the molecular weight is less than 3kDa), wherein the active peptide accounts for 1-5% of the total weight of the composition. The preparation method comprises the following steps: performing composite enzymolysis on pumpkin seed protein, performing ultrafiltration separation, performing anion exchange chromatography purification to obtain C2-1 active peptide (KRGGRFL), and homogenizing the KRGGRFL and fish oil to obtain the stable dispersion. The composition significantly reduces serum total cholesterol (TC), triglyceride (TG) and low density lipoprotein cholesterol (LDL-C) by synchronously inhibiting cholesterol esterase and lipase activity, and improves high density lipoprotein cholesterol (HDL-C). Animal experiments show that the lipid-lowering effect of the fish oil is superior to that of single fish oil and is equivalent to that of chemical drugs. The polypeptide-fish oil synergistic interaction is realized for the first time, and a safe and efficient new scheme is provided for hyperlipidemia prevention and treatment.

This invention belongs to the field of comprehensive utilization of food by-products and condiment processing technology, specifically relating to a method for preparing a seasoning base material of rice wine lees based on the directional control of the Maillard reaction through hot air drying and its application. Pretreated rice wine lees are hot-air dried at 100-140℃, with controlled temperature and time. Lees treated at 100℃ exhibit reduced bitterness and optimal umami, salty-savory balance, and overall acceptability, making them suitable as a high-quality auxiliary ingredient in compound seasonings such as chicken essence. Lees treated at 140℃ show increased ABTS free radical scavenging rate and inhibitory activity against pancreatic lipase and cholesterol esterase, possessing both antioxidant and lipid-lowering functions. This invention achieves directional control of the Maillard reaction solely through precise temperature control of hot air drying, eliminating the need for added exogenous amino acids or sugars. This transforms rice wine lees, a low-value by-product, into a seasoning base material with characteristic flavor and functional activity, significantly increasing added value.

The invention belongs to the technical field of oak functional development, and particularly relates to a preparation method and application of white oak essential oil. The white oak essential oil provided by the invention can up-regulate the expression level of genes participating in lipid hydrolysis, such as atgl-1 and hosl-1, and genes participating in fatty acid beta oxidation, such as acs-2, and down-regulate the expression level of genes participating in lipid synthesis, such as fat-5, fat-6, fat-7 and sbp-1, so that lipid hydrolysis is promoted, fatty acid beta oxidation is promoted, and lipid synthesis is inhibited; in addition, the oak can be combined with pancreatic lipase through reversible chemical bonds, the activity of pancreatic lipase, cholesterol esterase and alpha-glucosidase is inhibited, synthesis of blood fat components such as triglyceride is inhibited, the blood fat reducing effect is achieved, and therefore the technical problem that existing oak is low in functional development degree is solved.

The invention relates to the technical field of natural medicinal chemistry, and particularly discloses application of a traditional Chinese medicine extract in preparation of a lipid-lowering product. The traditional Chinese medicine extract is prepared from broadleaf holly leaves, lotus leaves, licorice roots and Chinese dates. The preparation method comprises the following steps: mixing the broadleaf holly leaves, the lotus leaves, the liquorice and the Chinese dates, and extracting with water; after the extraction is finished, taking an extracting solution, concentrating and drying to obtain a water extract, and taking the water extract to obtain the traditional Chinese medicine extract. Researches show that the traditional Chinese medicine extract disclosed by the invention has an inhibition effect on cholesterol esterase and pancreatic lipase; therefore, when being used as an active part for preparing a lipid-lowering product for treating and / or preventing hyperlipidemia, the compound has an important application value.

The invention discloses dry chemical test paper and application thereof. The dry chemical test paper comprises a supporting layer, a blood filtering layer, a filtering layer and a reaction layer, the filtering layer is treated by an R1 reagent, and the reaction layer is treated by an R2 reagent; the R1 reagent comprises a 3-morpholine propanesulfonate buffer solution, polyanions, an Mg < 2 + > reagent and a thickening agent; the R2 reagent comprises a 3-morpholine propanesulfonate buffer solution, a nonionic surfactant, an auxiliary surfactant, a saccharide stabilizer, a protein stabilizer, an auxiliary stabilizer, a preservative, a color developing agent, a chromogen substrate, peroxidase, cholesterol oxidase and cholesterol esterase. The dry chemical test paper can directly detect the low-density lipoprotein cholesterol on the dry chemical film, is not influenced by the concentration of triglyceride in a sample, and is accurate in detection result.

The invention provides a lipoprotein subfraction detection kit and a lipoprotein subfraction detection system, and belongs to the technical field of medical examination.The lipoprotein subfraction detection kit comprises a reagent R1, a reagent R2 and a reagent R3, the reagent R1 comprises a first buffer solution, a surfactant, magnesium chloride, a first protective agent, a first preservative, 4-aminoantipyrine, lipoprotein esterase, cholesterol esterase and ascorbic acid oxidase; the reagent R2 comprises a second buffer solution, a second protective agent, a second preservative, NAD < + > and glycerol dehydrogenase; the reagent R3 comprises a third buffer solution, a third protective agent, a third preservative, cholesterol oxidase, peroxidase and a chromogen substance. The lipoprotein subfraction detection kit provided by the invention is matched with the lipoprotein subfraction detection system provided by the invention, so that synchronous detection of cholesterol and triglyceride can be realized, and further detection of 20 lipoprotein subfractions is realized.

The present invention discloses an active peptide having both lipid-lowering and uric acid-lowering functions, and its preparation method and application, which belong to the field of biotechnology. The preparation method of the active peptide of the present invention comprises the following steps: taking quinoa powder, adding ethanol, reacting, and drying to obtain defatted quinoa powder; adding alkali solution, reacting; filtering and centrifuging the reaction solution, collecting the supernatant and adjusting, standing and then centrifuging, removing the supernatant, and retaining the protein precipitate; freeze-drying to obtain a quinoa protein crude extract; adding water to form a solution, adding protease for enzymolysis to obtain an enzymolysis solution; terminating the enzymolysis reaction, centrifuging, and collecting the supernatant. The active peptide provided by the present invention has good results in three in vitro lipid-lowering activity evaluation indicators: pancreatic lipase inhibition, sodium taurocholate binding ability, and cholesterol esterase inhibition. In addition, the xanthine oxidase inhibition effect of the quinoa protein hydrolysate shows that the quinoa protein hydrolysate under the optimal process conditions has good uric acid-lowering activity.

This invention discloses the preparation and application of a recombinant cholesterol esterase immobilized enzyme preparation. This invention will... Ophiostoma piceae Cholesterol esterase mutant gene ope-D136P Linked to pPICZαA plasmid, via signal peptide replacement and rDNA Gene insertion yielded the pEESB-3 plasmid; the protein disulfide isomerase gene was inserted. pdi The pPIC9K plasmid was ligated to obtain the pEESB-4 plasmid. This plasmid was electroporated into Pichia pastoris GS115, and after resistance selection and methanol-induced expression selection, recombinant Pichia pastoris engineered strains were obtained. Recombinant cholesterol esterase was obtained from this strain after methanol-induced expression. An aminated immobilization vector was prepared using APTES and TEOS as silane precursors. After grafting with 2,4-dihydroxyacetophenone and nickel ion chelation, the recombinant cholesterol esterase was immobilized to obtain an immobilized enzyme preparation. This immobilized enzyme preparation can catalyze the synthesis of stigmasterol ferulic acid ester from stigmasterol in a non-aqueous phase system, with a conversion rate of 33% (based on stigmasterol) or 32% (based on ferulic acid).

The application discloses a kind of based on multi-source feature fusion and multi-task collaborative learning lipid digestion double-target point inhibitory peptide prediction method;Belong to bioinformatics and functional food development technical field, for the single enzyme target and ignore pancreatic lipase (PL) and cholesterol esterase (CE) of the problem of existing method, the present method constructs the isomerous data set containing PL and CE inhibitory activity, allows the single target activity loss of existence of part sample;19 core molecular descriptors are fused to represent the multi-dimensional space conformation, electrostatic anchoring capacity and hydrophobic channel filling characteristics of polypeptide, to construct multi-source feature fingerprint;On this basis, the extreme random tree (ExtraTrees) algorithm is introduced to construct a multi-task collaborative regression model, and the potential correlation mechanism between the double targets is mined using unaligned data, and the model robustness is enhanced by combining the bidirectional noise injection strategy, the present application realizes the synchronous accurate prediction (R2>0.9) of the double inhibition activity of polypeptide, and significantly improves the screening efficiency and success rate of broad-spectrum lipid-lowering active peptides.

The invention discloses soybean-derived cholesterol-lowering polypeptide as well as a preparation method and application thereof, and relates to the technical field of biology. The amino acid sequence of the soybean-derived cholesterol-lowering polypeptide is shown as any one of SEQ ID NO.1-2. The invention further discloses a preparation method of the soybean-derived cholesterol-lowering polypeptide. The two kinds of functional polypeptides obtained through screening are derived from natural plant soybeans and have a definite cholesterol lowering effect, the action mechanism of the polypeptides is that the activity of cholesterol esterase is specifically inhibited, hydrolysis and absorption of dietary cholesterol ester are reduced, then the cholesterol level in the body is reduced, and the cholesterol lowering effect is achieved. The action is doubly verified through a molecular docking technology and an in-vitro enzyme activity inhibition experiment, the mechanism is clear, and the action target is definite. In view of the characteristics, the two polypeptides as natural cholesterol-lowering active components can be directly used as functional factors to develop functional food and products in the related medicine field, which are beneficial to maintaining the health level of blood fat (cholesterol), and have remarkable application value and wide market prospect.

The application discloses a cholesteryl esterase inhibiting peptide activity prediction method, comprising the following steps: step one, constructing an initial data set and performing abnormal value cleaning; step two, feature dimension reduction and core feature screening; step three, feature representation based on a physical mechanism; step four, small sample data enhancement; and step five, constructing a prediction model and activity prediction. The application innovatively introduces a strategy combining "double model consensus elimination" and "Gaussian noise enhancement", which can effectively remove outliers caused by experimental operation errors, ensure the purity of training data, and significantly reduce the overfitting risk of the model on small samples. The advantages of integrating random forest and XGBoost are used for feature screening, so that the selected features have strong robustness. Meanwhile, the "electrostatic anchoring + hydrophobic channel filling" mechanism of cholesteryl esterase inhibiting peptides makes the model not only have high prediction accuracy, but also have strong biological interpretability, which can effectively guide subsequent molecular design.