19 results about "Cholesterol Esterase" patented technology

The present disclosure provides polypeptides having a cholesterol-lowering function in a soybean protein hydrolysate, a preparation method thereof, and uses thereof. The polypeptides are derived from peptide fragments having a molecular docking “binding energy” value of less than −1.2 kcal with cholesterol esterase. The peptide fragments of the polypeptides that are subjected to molecular docking with cholesterol esterase are derived from 23 polypeptides having a Peptide Ranker bioactivity score of greater than 0.9. The polypeptides possess a significant blood-lipid-lowering function.

According to the invention, the traditional fermented koumiss in Xinjiang is collected, two novel multifunctional probiotic strains, namely, the lactobacillus plantarum SR6 GDMCC NO.66817 strain and the enterococcus faecalis SM5 GDMCC NO.66816 strain with good compatibility are separated and screened out, and the two strains have the characteristics of reducing blood fat, reducing cholesterol, removing triglyceride, resisting oxidation and antagonizing pathogenic bacteria, and have the characteristics of good cholate and gastrointestinal tract tolerance, high survival rate and good safety. The growth characteristics and compatibility of the two strains are improved; the inhibition rate of cholesterol esterase and pancreatic lipase; the method comprises the following steps: evaluating the triglyceride removal rate, antioxidant activity, pathogenic bacterium resistance, artificially simulated gastrointestinal fluid tolerance, strain drug resistance and safety, compounding lactobacillus plantarum SR6 and enterococcus faecalis SM5 strains according to a ratio of 1: 1 to prepare a freeze-dried bacterial agent, and keeping good survival rate (pgt; 0.05) and functional characteristics, the strain can be used as a functional direct vat set starter and is directly applied to production of fermented dairy products, and the biological activity of food is improved.

The application provides a recombinant cholesteryl esterase, a polynucleotide thereof, a recombinant plasmid, an expression system and application. The application belongs to the technical field of protein engineering, and specifically, the amino acid sequence of the recombinant cholesteryl esterase provided by the application is shown as SEQ ID No. 1, and the nucleotide sequence for encoding the recombinant cholesteryl esterase is shown as SEQ ID No. 2. The sequence of the recombinant cholesteryl esterase provided by the application has a full length of 482 amino acids, is derived from Aspergillus ruber, has the advantage of high thermal stability, and has a thermal stability deviation of less than 10% after thermal storage at 37 DEG C for 11 days and after 30 days of on-board opening, and has a wide application prospect in the field of in-vitro diagnostic reagents.

The application belongs to the technical field of biological processing, and specifically discloses a sea cucumber flower polypeptide with lipid-lowering efficacy as well as a preparation method and application thereof. The sea cucumber flower polypeptide with lipid-lowering efficacy is composed of short peptides with a molecular weight less than 1000 Da; the short peptides are one or more of GTGATGTF, FTGIVGSL, LAIGETEF, VGITDIESF, VDDEF and YDDVP. The application discloses a sea cucumber flower polypeptide with lipid-lowering efficacy as well as a preparation method and application thereof. The sea cucumber flower polypeptide has a significantly improved inhibition rate on cholesteryl esterase and pancreatic lipase, and has a stronger adsorption capacity on bile acid. The sea cucumber flower polypeptide does not contain the enzyme cutting sites of pepsin and trypsin, can resist secondary digestion and degradation after being taken into the human body, stably maintains structural integrity and biological activity, and solves the problem that traditional lipid-lowering peptides are easily destroyed in activity.

The invention discloses a compound enzyme solution for improving total cholesterol test current, which is prepared from the following components in percentage by weight: 20 to 200mM of buffer solution, 0.5 to 200mg / mL of cholesterol oxidase, 0.1 to 100mg / mL of cholesterol esterase, 0.1 to 25mg / mL of peroxidase, 1 to 6 percent of potassium ferricyanide, 0.1 to 8 percent of glucoside and the balance of water. And the cellulose accounts for 0.01%-2%. When a whole blood sample is tested, the compound enzyme liquid of lipoprotein can be quickly decomposed, so that the test current is improved, and the test time is shortened.

The invention belongs to the technical field of functional microorganism application, and particularly relates to a novel lipid-lowering peptide derived from a probiotic thallus and application thereof, the novel lipid-lowering peptide is a crushed substance derived from a probiotic lactobacillus plantarum thallus, and the molecular weight of the novel lipid-lowering peptide is less than 3kDa. And two key polypeptides APGFGDR (AP7) and TESGAFGR (TE8) are identified from the components, and can specifically inhibit the activities of pancreatic lipase and pancreatic cholesterol esterase. The extracted thallus peptide can be efficiently combined with cholate to inhibit the formation of cholesterol micelles, and has a remarkable inhibiting effect on pancreatic lipase and pancreatic cholesterol esterase. Animal experiments show that the mycopeptide can effectively improve lipid metabolism disorder induced by high fat diet, inhibit weight gain, reduce serum and liver lipid levels and relieve fatty degeneration and inflammation of the liver. The lipid-lowering peptide disclosed by the invention is high in safety and multi-target in action, and can be used for preparing functional products for preventing or treating metabolic diseases such as obesity, hyperlipidemia and the like.

The present application relates to a kind of fish oil polypeptide composition for reducing blood fat and its application, belong to the field of biological medicine and functional food.The composition includes fish oil and pumpkin seed source active peptide C2-1 (molecular weight <3kDa), wherein active peptide accounts for 1-5% of the total weight of composition.Its preparation method includes: pumpkin seed protein complex zymolysis, ultrafiltration separation, anion exchange chromatography purification obtains C2-1 active peptide (KRGGRFL), then with fish oil homogenization into stable dispersion.The composition significantly reduces serum total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) by simultaneously inhibiting cholesterol esterase and lipase activity, and improves high-density lipoprotein cholesterol (HDL-C).Animal experiments show that its lipid-lowering effect is better than single fish oil, and is comparable to chemical drugs.The present application first realizes polypeptide-fish oil synergistic effect, provides safe and efficient new scheme for hyperlipidemia prevention and treatment.

The invention discloses selenium-enriched spirulina carbon dots as well as a preparation method and application thereof, and belongs to the technical field of medicines. The selenium-rich spirulina powder is obtained by taking selenium-rich spirulina growing to a logarithmic phase as a raw material, centrifuging, collecting and freeze-drying. Uniformly mixing the spirulina platensis with a catalyst and deionized water according to a mass ratio of 1: (0.1-0.8): 20, then putting the mixture into a high-temperature reaction kettle to react at 140-260 DEG C for 1.5-5.5 hours, dialyzing the reaction liquid for 24 hours after the reaction is finished, centrifuging, and freeze-drying the dialysate to obtain the selenium-enriched spirulina platensis carbon dots with the yield of 70-95%. The selenium-enriched spirulina carbon dots provided by the invention not only have the inhibition capability of alpha-amylase, alpha-glucosidase, cholesterol esterase and pancreatic lipase and the cholate binding capability, but also can improve the endogenous antioxidant defense capability of model organisms while removing exogenous oxidizing substances, so that the glycolipid metabolism balance is efficiently regulated and controlled.

This invention discloses a dry chemical reagent for total cholesterol detection, comprising a diffusion layer, a spacer layer, and a reagent layer sequentially overlapped. The diffusion layer includes reflective particles, a binder, a surfactant, cholesterol esterase, and cholesterol oxidase. The reagent layer includes a carrier, horseradish peroxidase, an indicator composition, and a buffer solution. The dry chemical detection reagent of this invention employs a coating method, layering a premixed homogeneous slurry onto a support layer. A spacer layer blocks interlayer interference, and phenolic substances replace photosensitizers as chromogens, combined with a specific ratio of substrate and buffer system. The multi-layered, spacer-free, low-interference vertical detection system constructed by this invention significantly improves the repeatability, accuracy, and stability of the detection reagent.

The invention provides Porro lactobacillus mucus with a lipid-lowering function and application of the Porro lactobacillus mucus, and belongs to the technical field of microorganisms. The invention relates to a lactobacillus mucus strain FVT08 of Porro, and the preservation number of the lactobacillus mucus strain FVT08 is CGMCC (China General Microbiological Culture Collection Center) No.33640. The strain FVT08 not only has high lipid-lowering capacity under in-vitro conditions, can inhibit the activity of lipase (pancreatic lipase and cholesterol esterase) to reduce fat absorption of a body, but also has high bile salt hydrolase activity to promote cholesterol discharge, and at the animal level, the strain FVT08 colonizes in intestinal tracts to promote cholesterol discharge. The liver blood fat level and the liver tissue antioxidant level are reduced, lipid metabolism related gene expression and intestinal flora disorder are improved, and the content of short-chain fatty acids (propionic acid, n-butyric acid, isobutyric acid, n-valeric acid and isovaleric acid) can be increased; meanwhile, the Lactobacillus mucilaginosus FVT08 of Porro can obviously reduce the expression level of hepatic tissue inflammatory factors and improve hepatic tissue structure damage caused by obesity.

The invention relates to the technical field of medicines, and provides a triterpenoid compound in ganoderma leukemia as well as a preparation method and application of the triterpenoid compound. Adding pure methanol according to a solid-liquid ratio of 1: 10, extracting at 60 DEG C for 2h (hour, hour), filtering to obtain a methanol extracting solution, repeating for three times, and combining filtrates; concentrating the filtrate to 1 / 5 of the original volume at 50 DEG C, and adding distilled water with the volume fraction of 2 / 3 until the methanol concentration is 60% to obtain a preparation raw material; the preparation raw materials are subjected to coarse separation in a C18 column. The invention proves that the DM (12-O-Deacetuleuccontextin M) has antioxidant activity, can inhibit the activity of alpha-glucosidase, cholesterol esterase and pancreatic lipase, and shows a multi-target lipid-lowering potential. In a high glucose induced nematode model, the DM can be used for effectively reducing lipid accumulation and lowering the level of TG (triglyceride) and NEFA (non-esterified fatty acid), and the DM can be used for effectively reducing the level of TG (triglyceride) and NEFA (non-esterified fatty acid).

Functional minerals produced from plant ash offer new applications, including the promotion of lipase enzyme activity, the promotion of cholesterol esterase or cholesterol oxidase enzyme activity, and the promotion of micronization of oil droplets of oily substances. [Solution] A plant ash selected from wood ash, grass ash, and seaweed ash obtained by burning trees, grasses, and seaweeds respectively is used as a raw material. A mixed ash obtained by mixing seaweed ash, or 50% by weight or more of seaweed ash, with at least one of wood ash and grass ash, is reheated, and a mineral-containing lipase enzyme activity enhancer, cholesterol esterase or cholesterol oxidase enzyme activity enhancer, and oil droplet micronizer are obtained by extracting the resulting reheated ash with water.

This invention belongs to the field of comprehensive utilization of food by-products and condiment processing technology, specifically relating to a method for preparing a seasoning base material of rice wine lees based on the directional control of the Maillard reaction through hot air drying and its application. Pretreated rice wine lees are hot-air dried at 100-140℃, with controlled temperature and time. Lees treated at 100℃ exhibit reduced bitterness and optimal umami, salty-savory balance, and overall acceptability, making them suitable as a high-quality auxiliary ingredient in compound seasonings such as chicken essence. Lees treated at 140℃ show increased ABTS free radical scavenging rate and inhibitory activity against pancreatic lipase and cholesterol esterase, possessing both antioxidant and lipid-lowering functions. This invention achieves directional control of the Maillard reaction solely through precise temperature control of hot air drying, eliminating the need for added exogenous amino acids or sugars. This transforms rice wine lees, a low-value by-product, into a seasoning base material with characteristic flavor and functional activity, significantly increasing added value.

The invention relates to the technical field of natural medicinal chemistry, and particularly discloses application of a traditional Chinese medicine extract in preparation of a lipid-lowering product. The traditional Chinese medicine extract is prepared from broadleaf holly leaves, lotus leaves, licorice roots and Chinese dates. The preparation method comprises the following steps: mixing the broadleaf holly leaves, the lotus leaves, the liquorice and the Chinese dates, and extracting with water; after the extraction is finished, taking an extracting solution, concentrating and drying to obtain a water extract, and taking the water extract to obtain the traditional Chinese medicine extract. Researches show that the traditional Chinese medicine extract disclosed by the invention has an inhibition effect on cholesterol esterase and pancreatic lipase; therefore, when being used as an active part for preparing a lipid-lowering product for treating and / or preventing hyperlipidemia, the compound has an important application value.

The invention discloses dry chemical test paper and application thereof. The dry chemical test paper comprises a supporting layer, a blood filtering layer, a filtering layer and a reaction layer, the filtering layer is treated by an R1 reagent, and the reaction layer is treated by an R2 reagent; the R1 reagent comprises a 3-morpholine propanesulfonate buffer solution, polyanions, an Mg < 2 + > reagent and a thickening agent; the R2 reagent comprises a 3-morpholine propanesulfonate buffer solution, a nonionic surfactant, an auxiliary surfactant, a saccharide stabilizer, a protein stabilizer, an auxiliary stabilizer, a preservative, a color developing agent, a chromogen substrate, peroxidase, cholesterol oxidase and cholesterol esterase. The dry chemical test paper can directly detect the low-density lipoprotein cholesterol on the dry chemical film, is not influenced by the concentration of triglyceride in a sample, and is accurate in detection result.

This invention discloses the preparation and application of a recombinant cholesterol esterase immobilized enzyme preparation. This invention will... Ophiostoma piceae Cholesterol esterase mutant gene ope-D136P Linked to pPICZαA plasmid, via signal peptide replacement and rDNA Gene insertion yielded the pEESB-3 plasmid; the protein disulfide isomerase gene was inserted. pdi The pPIC9K plasmid was ligated to obtain the pEESB-4 plasmid. This plasmid was electroporated into Pichia pastoris GS115, and after resistance selection and methanol-induced expression selection, recombinant Pichia pastoris engineered strains were obtained. Recombinant cholesterol esterase was obtained from this strain after methanol-induced expression. An aminated immobilization vector was prepared using APTES and TEOS as silane precursors. After grafting with 2,4-dihydroxyacetophenone and nickel ion chelation, the recombinant cholesterol esterase was immobilized to obtain an immobilized enzyme preparation. This immobilized enzyme preparation can catalyze the synthesis of stigmasterol ferulic acid ester from stigmasterol in a non-aqueous phase system, with a conversion rate of 33% (based on stigmasterol) or 32% (based on ferulic acid).

The application discloses a kind of based on multi-source feature fusion and multi-task collaborative learning lipid digestion double-target point inhibitory peptide prediction method;Belong to bioinformatics and functional food development technical field, for the single enzyme target and ignore pancreatic lipase (PL) and cholesterol esterase (CE) of the problem of existing method, the present method constructs the isomerous data set containing PL and CE inhibitory activity, allows the single target activity loss of existence of part sample;19 core molecular descriptors are fused to represent the multi-dimensional space conformation, electrostatic anchoring capacity and hydrophobic channel filling characteristics of polypeptide, to construct multi-source feature fingerprint;On this basis, the extreme random tree (ExtraTrees) algorithm is introduced to construct a multi-task collaborative regression model, and the potential correlation mechanism between the double targets is mined using unaligned data, and the model robustness is enhanced by combining the bidirectional noise injection strategy, the present application realizes the synchronous accurate prediction (R2>0.9) of the double inhibition activity of polypeptide, and significantly improves the screening efficiency and success rate of broad-spectrum lipid-lowering active peptides.

The application discloses a cholesteryl esterase inhibiting peptide activity prediction method, comprising the following steps: step one, constructing an initial data set and performing abnormal value cleaning; step two, feature dimension reduction and core feature screening; step three, feature representation based on a physical mechanism; step four, small sample data enhancement; and step five, constructing a prediction model and activity prediction. The application innovatively introduces a strategy combining "double model consensus elimination" and "Gaussian noise enhancement", which can effectively remove outliers caused by experimental operation errors, ensure the purity of training data, and significantly reduce the overfitting risk of the model on small samples. The advantages of integrating random forest and XGBoost are used for feature screening, so that the selected features have strong robustness. Meanwhile, the "electrostatic anchoring + hydrophobic channel filling" mechanism of cholesteryl esterase inhibiting peptides makes the model not only have high prediction accuracy, but also have strong biological interpretability, which can effectively guide subsequent molecular design.