67 results about "Ros scavenging" patented technology

The invention relates to the field of gene engineering and molecular breeding, and discloses application of a ZmTCP5 gene in regulation and control of heat resistance of corn. According to the invention, three types of knockout mutants of the gene are obtained by using a CRISPR / Cas9 technology, and three overexpression materials are created. At normal temperature (25-28 DEG C), the material has no obvious phenotypic difference with a wild type; however, under high temperature stress (45 DEG C), the ZmTCP5 knockout mutant has enhanced heat resistance, reduced reactive oxygen species (ROS) accumulation, and significantly increased ROS scavenging enzyme activity, chlorophyll content and photochemical efficiency; on the contrary, the ZmTCP5 overexpression strain is more sensitive to heat stress, which indicates that the ZmTCP5 negatively regulates the heat resistance of the corn. The research provides a theoretical basis for analyzing a heat-resistant molecular mechanism of the corn, and also provides an important gene resource for creating a new germplasm of the heat-resistant corn.

The invention relates to the technical field of composite antibacterial materials, in particular to a preparation method and application of a spike rod-shaped nano composite material Cu-Fe-MOF (at) Ag. Rod-shaped Fe-MOF with biocompatibility is used as a carrier, Cu < 2 + > is introduced, and the appearance of the Fe-MOF is adjusted into a spike type, so that the antibacterial activity of the Fe-MOF is enhanced. On the basis of a soft and hard acid-base theory, by utilizing the principle that a d electron orbit of nano Ag is full electrons and is easy to coordinate with O and N atoms in Cu-Fe-MOF, the rodlike spike Cu-Fe-MOF (at) Ag composite nano material which can be adaptive to infected wound healing and intelligently adjust antibacterial activity and ROS (reactive oxygen species) removal capability is prepared through dual effects of adsorption and coordination. According to the material, the antibacterial activity of nano Ag can be improved, due to existence of coordinate bonds, the problem that Ag NPs are difficult to agglomerate and recycle can be solved, the problem of toxicity caused by accumulation of nano Ag on infected wounds is solved, and meanwhile, under the alkaline condition, conjugated bonds in MOF can cooperate with Ag to improve the ROS removal capacity.

The invention discloses an imitated intercorial nano preparation for treating acute respiratory distress syndrome, and belongs to the technical field of biological medicine. The internalized nano preparation comprises an active component, a liposome phospholipid bilayer and a target head, wherein the active component consists of an ROS (reactive oxygen species) removing drug and an anti-inflammatory drug. According to the invention, efficient and accurate targeting of the nano-preparation is realized by innovatively applying an imitated interment effect, an apoptotic cell marker is modified on the surface of the liposome, and an'eat me 'signal is released to simulate a natural biological process that macrophages clear apoptotic cells to be recognized and swallowed by alveolar macrophages, so that efficient and specific targeting is realized, and the targeting effect of the nano-preparation is improved. Meanwhile, up-regulation of PS receptor expression of macrophages at the inflammation part further enhances the targeting effect, and a new way is provided for efficient delivery of acute respiratory distress syndrome medicine preparations and remodeling of pathological microenvironment.

Complexes of polyoxometalate (POM) cluster anions and metal oxide nanocrystal (NC) cores (POM-NC) with tunable NC surface protonation are disclosed. These protonated POM-NC complexes enable precise control over catalytic properties, enhancing catalytic activity under near-neutral pH conditions while improving stability and solubility across various applications. The protonated POM- metal oxide complexes facilitate the activation of airborne O2 under ambient conditions and the production of reactive oxygen species (ROS), including hydroxyl radicals, superoxide, and peroxides. In certain catalytic processes, these complexes mimic enzymatic functions, such as oxidase, peroxidase, catalase, and superoxide dismutase activity, making them valuable for various biological, therapeutic, chemical and environmental applications such as applications related to oxidative catalysis and ROS scavenging.

The invention relates to the field of biomedical materials, in particular to an Nb2C MXene composite functional film capable of promoting angiogenesis and a preparation method of the Nb2C MXene composite functional film, the Nb2C MXene composite functional film is particularly suitable for diabetes wound healing, and the Nb2C MXene composite functional film comprises an Nb2C MXene functionalized collagen vitrified film layer, and a carboxymethyl chitosan vitrification film layer, wherein a nano interlocking interface is formed between the Nb2C MXene functionalized collagen vitrified membrane layer and the carboxymethyl chitosan vitrified membrane layer; the Nb2C MXene functionalized collagen vitrified membrane layer is hydrophobic, the carboxymethyl chitosan vitrified membrane layer is hydrophilic, through the double-layer asymmetric structural design, the inner layer is tightly attached to a wound, the outer layer prevents adhesion of bacteria and tissues, and the barrier function in the wound healing process is effectively guaranteed; the adopted Nb2CMXene has excellent ROS (reactive oxygen species) removal capacity, oxidative stress of wound parts can be effectively relieved, cell damage is avoided, and cell functions are recovered.

The invention provides a self-adaptive multifunctional hydrogel system for cardiac microenvironment regulation after myocardial infarction as well as a preparation method and application of the self-adaptive multifunctional hydrogel system, and belongs to the technical field of biomedical materials. The hydrogel system is hydrogel prepared by taking a micromolecular cross-linking agent, a polymer matrix and drug-loaded microspheres as raw materials. The hydrogel can realize staged response drug release in oxidative stress, inflammation and fibrosis microenvironments after myocardial infarction, has good injectability and self-healing property, and has the functions of ROS removal, nitric oxide release and the like. The invention provides a new strategy for tissue repair after myocardial infarction with multi-channel collaboration and accurate pathological response control.

The present invention relates to a food composition for improving obesity or cognitive function, comprising a hemp seed fermentate prepared using the Pediococcus acidilactici SRCM201591 strain. The fermentate was confirmed for radical scavenging ability, ROS scavenging ability, and AChE inhibitory ability, and found to have an antioxidant effect as a high content of polyphenol was measured. An anti-obesity effect was also confirmed through assays for triglyceride inhibition and C. elegans size reduction, and antioxidant and anti-obesity related gene regulation effects were identified following administration of the fermented extract. Accordingly, the fermentate can be advantageously used in the manufacture of food for improving obesity or cognitive function.

The application relates to kaempferol cerium nanoparticles and a preparation method and application thereof, and belongs to the technical field of biological medicines. The kaempferol cerium nanoparticles are prepared by performing coordination co-assembly on kaempferol and cerium ions in the presence of polyethylene glycol at a specific molar ratio, and the nanoparticles have uniform particle sizes and good dispersity. The nanoparticles have the anti-inflammatory activity of kaempferol and the ROS scavenging capacity of cerium ions, and show a significant synergistic effect in in-vivo and in-vitro experiments, can effectively reduce lung inflammation, inhibit oxidative stress, improve lung function, and have good biological safety, thereby providing a new drug candidate scheme for the treatment of ARDS.

The invention discloses a phthalocyanine-based MOF nano material and a preparation method and application thereof.The nano material is expressed as M1-M2-MOF and has a chemical structure shown in the formula (b), M1 is selected from Ru, Au, Ag, Rh, Pd and Ir and serves as central coordination metal of phthalocyanine; and M2 is selected from Zn, Mn, Cu, Co and Ni and is used as a coordination metal for connecting a phthalocyanine unit. The preparation method comprises the following steps: dissolving beta-(OH) 8M1Pc in an organic solvent, and adding alkali; diluting the obtained solution with water, adding a surfactant, and carrying out ultrasonic treatment; mixing the obtained solution with an M2 salt solution, then transferring into a high-pressure kettle for heating reaction to obtain a reaction solution, cooling, centrifuging, sequentially washing with a reaction solvent, H2O and acetone, crushing, and collecting the liquid; metal nodes and ligands in the phthalocyanine-based MOF nano material are arranged in order, and the phthalocyanine-based MOF nano material has an efficient ROS removal function.

The invention relates to the technical field of botany, in particular to a method for improving salt tolerance of apocynum venetum. The invention provides a molecular module AvSnRK1.2-AvNAC69-Tre with a sequence as shown in SEQ ID NO: 1 and application of the molecular module AvSnRK1.2-AvNAC69-Tre in improvement of the salt tolerance of apocynum venetum, specifically, the expression of a trehalose synthesis key gene AvTPPD can be regulated and controlled through phosphorylation modification, and a trehalose biosynthesis pathway is activated, so that the osmotic regulation capability and ROS removal efficiency are enhanced, and the salt tolerance of the apocynum venetum is remarkably improved; and molecular module typing can be rapidly carried out on highly heterozygous parent individuals, and dominant genotypes are selected as parents, so that the salt tolerance of offspring is improved.

The invention relates to nano-engineered bacillus subtilis as well as a preparation method and application thereof, and belongs to the technical field of biology. Aiming at the technical problems of low probiotic delivery efficiency, insufficient regulation and control of enteropathogenic bacteria and single ROS (reactive oxygen species) removal in the prior art, a ternary composite system of chitosan-coated bacillus subtilis bridged WO3 (at) PDA nanoparticles is constructed. The preparation method comprises the following steps: carrying out auto-polymerization on dopamine hydrochloride and WO3 nanoparticles to form a WO3 (at) PDA core-shell structure; the method comprises the following steps: coating bacillus subtilis with chitosan through pH regulation to form BSCS; and finally, preparing the BSCS-coated WO3-coated PDA complex microbial inoculant through electrostatic interaction bridging. Experiments show that the preparation can relieve inflammatory symptoms of mice with DSS-induced colitis and effectively realize intestinal micro-ecological reprogramming. The invention provides a novel nano-probiotic synergistic treatment system for IBD treatment.

The invention discloses an antioxidant mesoporous organic silicon self-assembled nano material as well as a preparation method and application thereof, and belongs to the technical field of nano materials and biomedicine. The method comprises the following steps: S1, preparing an ROS removal micromolecule precursor TK-NH2 through a mercaptan condensation reaction; s2, preparing antioxidant mesoporous organic silicon spheres (PMOs) by taking TK-NH2 as a raw material through a sol-gel method; and S3, preparing the antioxidant mesoporous organic silicon self-assembled nano material (PMOs (at) Cur (at) Ca) by taking the PMOs as a raw material through an anti-solvent method. The method is simple in steps, normal in temperature and pressure, low in cost and capable of achieving large-scale production; the prepared antioxidant mesoporous organosilicon self-assembled nano material can be applied to preparation of drugs for treating inflammations, especially inflammatory bone diseases, by removing ROS (reactive oxygen species), especially drugs for treating rheumatoid arthritis.

The application discloses a kind of self-assembly nanomaterial and its application in ROS clearance.The self-assembly nanomaterial of the application is denoted as TPP-FFYp-O, and its structural formula is shown as follows:TPP-FFYp-O of the application is specifically cut to generate TPP-FFY-O by alkaline phosphatase (ALP) overexpressed after entering inflammatory cell, TPP-FFY-O is enriched near mitochondrion by the targeting effect of TPP, and is self-assembled to form nanoparticle and stays in inflammatory cell by π-π stacking.Nanoparticle surface enrichment of a large number of TEMPO can convert superoxide radical anion (O2 ·‑ ) in mitochondrion into hydrogen peroxide (H2O2), and then decompose into oxygen and water, thereby significantly reducing the ROS level in inflammatory cell.This enzyme-controlled self-assembly nanoparticle improves the stability of TEMPO and the ability of ROS clearance, and has good application prospect.

The invention discloses oral composite microspheres for treating ulcerative colitis based on intestinal ROS storm pathological axis regulation as well as a preparation method and application of the oral composite microspheres, and aims to solve the problems that an existing ulcerative colitis treatment means is difficult to reverse core pathological circulation, oral antioxidant drugs and nano-enzymes are easily inactivated by gastric acid, the targeting property is poor, and the treatment effect is poor. And when the probiotics are independently used, the oxidation resistance is insufficient. The oral composite microsphere is prepared by taking clostridium butyricum spores as a biological carrier, firstly loading Ir10-KB nano-enzyme with peroxidase, superoxide dismutase and catalase like activities, and then carrying out sodium alginate-calcium ion cross-linking embedding. Anti-gastric acid protection and colon targeted release are achieved through the pH response gel characteristic of the sodium alginate, the flora regulation and mucous membrane repair effects of clostridium butyricum spores and the efficient ROS removal, mitochondrial protection and PANoptosis inhibition functions of the Ir10-KB nano-enzyme are synergistically exerted, and the synergistic treatment purpose of precise delivery-efficient oxidation resistance-micro-ecological regulation is achieved.

The invention relates to the technical field of biomedical materials, in particular to an fCA-BExo composite material and a preparation method and application thereof. According to the complex, the cobalt-aluminum double hydroxide nanosheets are synthesized, mesenchymal stem cells are induced to secrete exosomes rich in bone morphogenetic proteins, fCA-BExo is formed, and the multiple effects of resisting oxidation, removing ROS, inhibiting inflammatory response and promoting osteogenic differentiation are achieved. The layered structure and surface electron vacancies of the nanosheet bring excellent ROS removal performance and biocompatibility, the exosome synergistically optimizes targeting and osteogenic induction effect, and the bone marrow microenvironment can be significantly improved. The complex can be efficiently dispersed in PBS or normal saline, is applied to osteoporosis diagnosis and treatment, and has the advantages of simplicity and convenience in operation, safety, high efficiency and transformation potential.

An arylalkylamine ferroptosis inhibitor based on an edaravone structure, and a preparation method therefor and a use thereof. The structural formula of the ferroptosis inhibitor is as shown in the following formula I, formula II, or formula III: formula I, formula II, formula III. The ferroptosis inhibitor has strong ROS scavenging ability and an inhibitory effect on lipid peroxidation, can inhibit ferroptosis caused by an ferroptosis inducer, and can reduce cerebral ischemia and nerve damage caused by cerebral ischemia, and alleviate symptoms of neurological diseases such as Parkinson's syndrome. On the basis of the ferrostain-1 / edaravone structure, the provided arylalkylamine ferroptosis inhibitor can exert a synergistic effect by means of multiple effects to treat ferroptosis-related diseases and can also expand the use of edaravone, thereby providing a candidate compound for subsequent drug research and development.

The application provides a preparation method of ROS-responsive hyperbranched polymer hydrogel and the hydrogel, and belongs to the technical field of hydrogel. Firstly, Michael addition reaction occurs between double bonds on PEGDA and amine groups of chain-like aliphatic diamine with ROS scavenging groups in the structure, and a hyperbranched polymer PBAE is obtained as a crosslinking agent; then esterification reaction occurs between methyl methacrylate and HA to obtain HAMA; finally, HAMA, AM and PBAE solution are mixed, and AHP hydrogel is formed through a one-step free radical polymerization reaction. The preparation method of the AHP hydrogel is simple, the material source is wide, the production efficiency is high, the AHP hydrogel has good mechanical properties, oxidation resistance and biocompatibility, and is easy to produce and prepare on a large scale.

The invention belongs to the field of biomedical nano materials, and discloses an ultra-small-size gold nano assembly (AuNAs) based on a micelle confinement in-situ growth strategy, a one-step preparation method and application. According to the assembly, an amphiphilic block copolymer pluronic F127 and n-dodecanethiol serve as a composite micelle template, reduction, nucleation and ordered assembly of a gold precursor are synchronously completed in a confinement space, the hydration particle size is 27.37 + / -4.95 nm, and the assembly is uniform in structure and excellent in stability. In-vitro experiments show that the assembly has efficient scavenging capacity on ABTS < + >, DPPH and OH (the scavenging rates are respectively 98.37%, 51.9% and 93.9% under the concentration of 10.0 nM), has no obvious toxicity on HK-2 cells within the concentration range of 0-20 nM, and can effectively remove excessive reactive oxygen species (ROS) in the cells. The preparation process is simple and convenient, the cost is controllable, the prepared AuNAs can be used for preparing ROS removal type cell protective agents or candidate drugs for treating oxidative stress related diseases, and the AuNAs have good industrial application prospects and clinical transformation potential.

The invention provides application of combined use of chlorogenic acid and copper ions in preparation of an external medicine for treating burn and scald wounds. The invention also provides an ROS responsive nano assembly with a network structure, which is characterized in that ROS responsive ketal thiol (TK) is covalently bound with hydrophobic chlorogenic acid (CGA) and a hydrophilic polymer mPEG 2000 to synthesize an amphiphilic polymer mPEG-TK-CGA (PTC), and then the amphiphilic polymer mPEG-TK-CGA (PTC) and Cu < 2 + > are self-assembled to form the PTC-coated Cu NPs. According to the invention, ROS removal, macrophage polarization and regeneration promotion functions are synergistically exerted, and the PTC-coated Cu NPs can effectively relieve oxidative stress, recover mitochondrial functions, reduce cell apoptosis and promote cell migration. The multifunctional linkage treatment strategy can break through pathological circulation of oxidative damage and chronic inflammation, and a comprehensive solution is provided for accelerated healing of infectious deep second-degree burns and scalds.

The application belongs to the technical field of pharmaceutical chemistry, and particularly relates to a gentiopicroside nitroxide radical derivative as well as a preparation method and application thereof. A series of gentiopicroside nitroxide radical derivatives with specific structures are prepared and synthesized through a chemical process. The derivatives exhibit good in-vitro COX-2 enzyme inhibition activity and in-vitro ROS scavenging activity. In-vivo and in-vitro experimental results show that each derivative can effectively inhibit the release of inflammatory mediators and oxidative stress during inflammation, thereby more effectively inhibiting the progress of inflammation and protecting tissues, and the derivatives have a wide application prospect in the preparation of drugs with the efficacy of preventing, relieving or treating inflammation.

The invention discloses a formaldehyde tolerance module based on an active oxygen ROS (reactive oxygen species) removal system and application, SodB and KatA are introduced into escherichia coli, and tolerance of cells to formaldehyde is remarkably enhanced. Formaldehyde is used as a toxic intermediate in methanol metabolism, a severe challenge is provided for research of methanol, and introduction of an active oxygen (ROS) removal system has a strong advantage on improvement of methanol assimilation ability. Engineering strains introduced into the ROS removal system show that the methanol assimilation level is obviously superior to that of control bacteria. Under the culture condition of 900mM methanol, the strain consumes about 200mM methanol and 300mM methanol respectively, which are 20 times and 30 times higher than those of a control strain without an ROS removal system respectively. In the efficient methanol assimilation strain, xylose and methanol are additionally added, so that methanol consumption is remarkably promoted.

The application discloses a 4-(benzo[d]thiazol-2-yl)benzene-1,2-ethylenediamine compound and a preparation method and application thereof. The structural formula of the compound is: the compound has strong ROS scavenging capacity, inhibits lipid peroxidation, can inhibit ferroptosis caused by an iron death inducer, can reduce brain ischemia and nerve damage caused by brain ischemia, and can relieve symptoms of Parkinson's syndrome and other neurological diseases. Therefore, the ferroptosis inhibitor based on the ferrostain-1 / lirilad structure provided by the application can treat diseases related to ferroptosis through multiple actions and has the potential to be developed into a drug for treating diseases related to ferroptosis. Moreover, the ferroptosis inhibitor can be used as an antioxidant fluorescent probe for high-sensitivity detection of a ferroptosis marker, lipid peroxide (PCOOH / PEOOH), can effectively reverse the ferroptosis process of cells, and has a self-indication capacity. The ferroptosis inhibitor is a solid powder, is convenient to store and use, has a simple synthesis method, high yield, low cost, and good popularization prospect.

The invention discloses a quercetin-based carbon dot with antibacterial and anti-inflammatory characteristics as well as a preparation method and application of the quercetin-based carbon dot. The preparation method of the quercetin-based carbon dots comprises the following steps: (1) S1, adding quercetin and 4-aminophenol into pure water, mixing, carrying out ultrasonic treatment, transferring the obtained mixed solution into a high-pressure reaction kettle, and reacting under heating; and (2) after the reaction is finished in the step (S2), cooling to room temperature, filtering, and freeze-drying filtrate to obtain the quercetin-based carbon dots (QA-CDs). The QA-CDs provided by the invention show a remarkable ROS (reactive oxygen species) removal capability and effectively regulate the expression level of inflammation-related factors; the QA-CDs can regulate the inflammation microenvironment while effectively exerting the antibacterial activity, so that the QA-CDs show obvious dual-function characteristics. A new scheme is established for application of flavone-based compounds, a promising research framework is provided for development of innovative bioactive materials with dual treatment effects, and development of innovative strategies in the field of bacterial infection treatment can be greatly promoted.

The application discloses oil-soluble PDRN and a preparation method and application thereof. The preparation method comprises the following steps: dissolving PDRN in an alkaline solvent to obtain a PDRN solution; adding an acylation agent into the PDRN solution to perform acylation modification, and precisely controlling pH in the whole process to obtain a reaction product liquid; performing salting-out and centrifugation on the reaction product liquid, and collecting a precipitate; and performing washing and vacuum drying on the precipitate to obtain oil-soluble PDRN. Through the acylation modification strategy of precisely controlling pH in the whole process, the oil-soluble PDRN with high acylation degree, excellent oil solubility, excellent ROS scavenging capacity, high DPPH free radical scavenging rate, excellent thermal stability and excellent light stability is successfully prepared, the oil-soluble PDRN can be dissolved in an oil phase matrix, and a technical breakthrough of improving oil solubility, retaining biological activity and enhancing antioxidant activity is realized.

This invention belongs to the field of nanomaterials technology, specifically relating to the preparation of nucleic acid-guided supramolecular semiquinone radical nanoparticles and their application in tumor photothermal therapy. The nucleic acid-guided supramolecular semiquinone radical nanoparticles provided by this invention are formed by non-covalent bonding of long single-stranded DNA containing repeating G-quadruplex units with polydopamine, and can be used to prepare tumor photothermal therapy reagents. This invention improves both the photothermal conversion capacity and ROS scavenging capacity of the nanomaterials by modifying the structure of polydopamine, achieving a synergistic effect in PTT (photothermal phototherapy).

This disclosure provides a method for alleviating aluminum toxicity in hydroponic peas based on menadione-mediated reactive oxygen species (ROS) metabolism. The core components include: regulating the concentration and duration of menadione treatment to optimize the spatiotemporal accumulation pattern of ROS (superoxide anion, hydrogen peroxide) in the roots of hydroponic peas; adjusting the dynamic response of endogenous antioxidant enzyme systems (SOD, POD, APX) to enhance menadione-mediated ROS scavenging capacity; controlling the initial ROS burst based on changes in plasma membrane NADPH oxidase (Rboh) gene expression and enzyme activity to inhibit the aluminum stress oxidative signaling cascade; and adjusting the timing and frequency of menadione administration based on subcellular-scale mitochondrial and chloroplast ROS leakage rates to alleviate organ-specific oxidative damage. This approach addresses five key issues: aluminum-induced oxidative damage, cellular redox imbalance, oxidative signaling cascade, organ-specific oxidative damage, and persistent aluminum toxicity metabolic disorders.

The present application relates to the field of biological medicine and tissue engineering, and particularly relates to a nano-enzyme functionalized dynamic hydrogel as well as a preparation method and application thereof.The nano-enzyme functionalized dynamic hydrogel comprises black phosphorus nanosheets and nano-enzymes.The preparation method of the nano-enzyme functionalized dynamic hydrogel comprises the following steps: (1) synthesis of a hydrogel precursor solution; (2) preparation of biomimetic nano-enzymes; and (3) preparation of a dynamic hydrogel.In the present application, BP can maintain Ce(III) / Ce(IV) redox cycling through self-oxidation and improve the ROS scavenging capacity in the microenvironment, thereby serving as CeO x a stabilizer of nano-enzymes.In addition, the MBC obtained by wrapping BP@CeO x in the nucleus pulposus cell membrane can better achieve nucleus pulposus cell targeting, so that BP@CeO x can effectively and durably scavenge intracellular ROS.

The application discloses a preparation method of a lysozyme-nano selenium compound and application of the lysozyme-nano selenium compound in ROS removal. To mix sodium selenite solution and lysozyme uniformly, a lysozyme-sodium selenite solution is obtained, then vitamin C is added, and the mixture is uniformly mixed under the condition of light shielding and at room temperature, the pH value is adjusted to 6.5-7.5, a red solution is obtained, and dialysis is performed, so that the lysozyme-nano selenium compound is obtained. The lysozyme-nano selenium compound has the advantages of simple process, mild preparation condition, higher stability, biological safety, efficient ROS removal in egg white, and no influence on the regulation ability of the functional property, edible quality and other characteristics of the egg white, so that the deterioration problem of the egg white in the storage process is solved. The lysozyme-nano selenium compound has good application prospect in ROS removal, and can be applied to the fields of biomedical science and material science.

The application provides an injectable black phosphorus nanosheet-loaded composite hydrogel and a preparation method and application thereof. The composite hydrogel is prepared by mixing polydopamine-functionalized black phosphorus nanosheets, a 2-2.5% wt / vol sodium alginate suspension, a 1.5-2% wt / vol calcium alginate suspension and hyaluronic acid; wherein the volume ratio of the 2-2.5% wt / vol sodium alginate suspension, the 1.5-2% wt / vol calcium alginate suspension and the hyaluronic acid is 5:5:2, and the concentration of the polydopamine-functionalized black phosphorus nanosheets in the synthesized composite hydrogel is 100 μg / ml; and the application of the injectable black phosphorus nanosheet-loaded composite hydrogel in a myocardial infarction disease injection drug, through ROS scavenging to inhibit the NFκB signal pathway and reduce inflammatory reactions, the black phosphorus nanosheet-loaded composite hydrogel in the application can be used as a ROS scavenging, anti-inflammatory, anti-apoptosis, conductive and biodegradable platform for tissue engineering applications.

The invention relates to a nano composite material with photo-thermal antibacterial property, ROS (reactive oxygen species) removal capability and capability of promoting wound healing and a preparation method of the nano composite material. The nano composite material is MnPB (at) PDA, and is formed by taking manganese ion doped Prussian blue nano cube MnPB as an inner core and wrapping the periphery of the inner core with polydopamine PDA. The average size of the nano composite material is 115-245 nm, the average size of the inner core MnPB is 95-200 nm, and the average thickness of the PDA shell layer is 10-23 nm. The preparation method comprises the following steps: (1) preparing MnPB by adopting a precipitation method; and (2) coating the PDA shell layer formed by dopamine auto-polymerization on the surface of the MnPB by adopting an in-situ polymerization method. The preparation process is simple, the product morphology is uniform, the controllability of composition and size is good, the product nano composite material has excellent photo-thermal antibacterial performance, good active oxygen species removal capacity and wound healing treatment effect, the antibacterial performance can be remarkably improved by adsorbing Cu < 2 + > or Zn < 2 + > ions, and the application prospect is wide. Good application prospects are realized in the treatment fields of photo-thermal antibiosis, ROS (reactive oxygen species) removal and wound healing promotion.