110 results about "Infected wound" patented technology

The invention discloses a macrocyclic covalent organic polymer material for inhibiting a pi-pi stacking effect and application of the macrocyclic covalent organic polymer material, and belongs to the technical field of biological medicines. The preparation method comprises the following steps: adding 5, 10, 15, 20-tetra (4-ethynylphenyl) copper porphyrin and a macrocyclic monomer into an organic solvent in a protective atmosphere, and carrying out solvothermal reaction in the presence of a catalyst and alkali to obtain the macrocyclic covalent organic polymer material. According to the invention, 5, 10, 15, 20-tetra (4-ethynylphenyl) copper porphyrin and a macrocyclic monomer form a porous polymer MCP-CU-POP, the material utilizes the photo-thermal capability to combine the effects of simulated photo-thermal (PTT), photodynamic (PDT), peroxidase (PODs) and glutathione peroxidase (GSH-Px) to perform synergistic antibiosis, and the inherent characteristics of an infected microenvironment can also be utilized to induce death of bacteria, so that the antibacterial property of the material is greatly improved, and the antibacterial property of the material is greatly improved. And the microenvironment of the bacterial infection part can be adjusted, so that the recovery of the bacterial infection wound can be obviously accelerated.

The invention belongs to the field of biological medicine and material science, and particularly relates to an intelligent response type piezoelectric nanofiber dressing and a preparation method and application thereof. According to the preparation method, polyvinylidene fluoride-trifluoroethylene, a high polymer material and inorganic nanoparticles are blended and spun through an electrostatic spinning technology, and then a photo-thermal component coating is subjected to surface in-situ oxidation and self-polymerization, so that the composite fiber membrane is prepared. Under the stimulation of ultrasound (US) and near-infrared laser (Laser), the fiber membrane can cooperatively activate the piezoelectric effect and the photothermal effect, generate a dynamic electric field and local high temperature, realize multi-mode cooperative killing of bacteria (the antibacterial rate exceeds 99%), and simulate an endogenous electric field to promote cell migration and wound healing. The dressing has good mechanical properties (the Young modulus is 15-20 MPa), hydrophilicity (the contact angle is about 30 degrees), biocompatibility and stability, is suitable for treatment of chronic infected wounds such as diabetes mellitus and the like, and has the advantages of on-demand activation and intelligent response.

The invention discloses a gel microneedle loaded with silver nanoparticles and a preparation method and application thereof.The extract nano-silver (AB-AgNPs) is prepared through a green synthesis strategy, a drug-loaded microneedle system is constructed based on carboxymethyl chitosan-vanillic aldehyde-polyvinyl alcohol (CMCS-Va-PVA) composite gel, the AB-AgNPs prepared through the technology are uniform in particle size and stable in dispersion, and the drug-loaded microneedle has the advantages that the drug-loaded microneedle is good in drug-loaded performance and good in drug-loaded performance; the crystal has a typical face-centered cubic crystal structure and metal state Ag0 characteristics; the gel microneedle has good mechanical strength and forming effect, the AB-AgNPs and the gel matrix are stably combined through hydrogen bonds, the chemical properties are kept unchanged, and the system has broad-spectrum efficient bacteriostatic activity and biocompatibility; an in-vitro experiment proves that the green synthesized gel microneedle loaded with the silver nanoparticles has a remarkable capability of promoting the healing of L929 cell tissues; a rat wound experiment proves that the gel microneedle can regulate and control an inflammatory factor network and promote cell migration and tissue regeneration, healing of common and infected wounds is remarkably accelerated, and the wound residue ratio in 9 days is as low as 2.7% and 10.9% respectively.

The invention discloses a covalent organic framework antibacterial material with NO light-controlled release as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The covalent organic framework antibacterial material is a porous polymer taking a cyclodextrin inclusion compound and a porphyrin metal coordination compound as structural units; a plurality of porphyrin metal coordination compounds surround the cyclodextrin inclusion compound in each structural unit, and the porphyrin metal coordination compounds are not on the same plane; the cyclodextrin inclusion compound is obtained by inclusion of BNN6 with beta-cyclodextrin; the porphyrin metal coordination compound is a coordination compound of porphyrin and copper. The preparation method comprises the following steps: firstly preparing a cyclodextrin porphyrin porous polymer, then matching the cyclodextrin porphyrin porous polymer with copper and entrapping BNN6 to obtain a covalent organic framework antibacterial material, utilizing NO release and combining simulated photo-thermal, photodynamic, peroxidase and glutathione peroxidase to achieve synergistic antibacterial effect, and remarkably accelerating the recovery of bacterial infected wounds.

The present invention pertains to use of sodium diacetate (NaHAC2) as an antimicrobial agent against bacteria growing in biofilms. The aspects of the invention include a wound care product comprising sodium diacetate, a kit comprising a wound care product, and a method of treating an infected wound.

A method for synergistically promoting wound healing by means of moderate-intensity pulse ultrasonic waves and zinc oxide nanoparticle-type-I collagen hydrogel comprises the following steps that zinc oxide nanoparticles and type-I collagen hydrogel are physically mixed and crosslinked in advance and applied to a chronic infected wound, and then the wound is accelerated to heal through multiple times of MIPUS stimulation. The effect of the ZNP-COL hydrogel is enhanced through MIPUS, immune cell polarization can be activated, and the proportion of anti-inflammatory M2 type macrophages is increased; wherein ZNP is capable of releasing zinc ions and reactive oxygen substances and has antibacterial activity; meanwhile, ZNP can generate current and an electric field under stimulation of MIPUS, calcium ions on the surface of a cell membrane are activated to flow inwards, and proliferation of anti-inflammatory M2 type macrophages is promoted; the COL hydrogel is used as a medium to transmit ultrasonic waves and fix the ZNP, and the function of the ZNP at the local position is enhanced. The method solves the problems of bacterial infection and chronic inflammation at the same time, is safe, simple and reusable, and can be widely applied to infection of other parts besides the application.

The application belongs to the field of hydrogel biological medicine, and more particularly discloses a kind of supramolecular hydrogel based on natural plant ingredients, preparation and application thereof.The hydrogel only contains polyphenol, polysaccharide and solvent, and the hydrogel is in-situ gelled only by supramolecular interaction between polyphenol and polysaccharide.The hydrogel of the application can be prepared by simple mixing, and can also be in-situ injected by a syringe.The hydrogel preparation process is simple, the raw material cost is low, and the gelation speed is fast;the hydrogel can effectively adhere to the skin, isolate the contact between the wound and the external environment, avoid the invasion of pathogens, at the same time, it is breathable and moisturizing, can provide a suitable moist environment for wound healing, and can completely and quickly release polyphenol.The hydrogel also has excellent antibacterial, antioxidant, anti-inflammatory and pro-angiogenic functions, so it can accelerate wound healing and accelerate bacterial infection wound healing.

The invention discloses an antibacterial hydrogel dressing taking spunlace non-woven fabric as a carrier and a preparation method of the antibacterial hydrogel dressing, and relates to the technical field of biomedical polymers. The antibacterial hydrogel dressing is composed of a spunlace non-woven fabric carrier layer and an antibacterial hydrogel functional layer loaded on the surface and in pores of the spunlace non-woven fabric carrier layer, and the preparation method comprises the following steps: sterilizing spunlace non-woven fabric to obtain a spunlace non-woven fabric carrier; adding the antibacterial component into the aqueous solution of the hydrophilic polymer base material to form a hydrogel precursor solution; and immersing the spunlace non-woven fabric carrier into the hydrogel precursor solution, and curing to obtain the antibacterial hydrogel dressing. The antibacterial hydrogel dressing prepared by the preparation method disclosed by the invention has the flexible breathable characteristic of spunlace non-woven fabric and the high swelling and antibacterial functions of hydrogel, has an excellent bactericidal effect on escherichia coli and staphylococcus aureus, can effectively promote healing of infected wounds, is suitable for clinical nursing of burn and traumatic skin injuries, and has wide application prospects. Wide clinical application prospects are realized.

The invention discloses a gallic acid-based cationic photodynamic antibacterial agent with sterilizing, anti-infection and wound healing promoting effects and a preparation method thereof, and belongs to the technical field of antibacterial agent synthetic chemistry. The cationic photodynamic antibacterial agent EY-QEGDM-MG is prepared by taking a natural compound gallic acid as a raw material and carrying out ring opening polymerization and quaternization reaction with photosensitizer eosin EY and ethylene glycol diglycidyl ether (EGDE), and the polymer shows an excellent antibacterial effect on escherichia coli and multi-drug-resistant MRSA germs under the action of illumination, and can be used for preparing a cationic photodynamic antibacterial agent EY-QEGDM-MG. The polymer has good blood compatibility and cell compatibility, in addition, the polymer can kill bacteria around infected wounds and can rapidly promote wound healing, and therefore the polymer can provide a new method for photodynamic antibacterial agents for treating the infected wounds in hospitals.

The invention discloses a microenvironment response type chessboard microneedle system and application thereof in treatment of drug-resistant bacteria infected wounds, and provides the microneedle system for solving the technical problems that in treatment of MRSA infected wounds, drugs are difficult to penetrate through wound surfaces and biological membrane barriers, the effective concentration of NO is difficult to maintain, and antibacterial-repair synergy is insufficient. The microneedle is formed by alternating hyaluronic acid and polyvinyl alcohol, ZCSO nano-enzyme and NO donor SNAP are loaded on the microneedle respectively, and the microneedle can penetrate through a physical barrier to accurately deliver functional components to a focus to achieve space targeting. The nidus microenvironment is triggered to release Cu < 2 + >, SNAP is catalyzed to continuously produce NO, time-space synchronization of NO and Cu < 2 + > is realized, and the NO gas therapy and copper-like death are used for synergistically resisting bacteria; se and Zn < 2 + > can relieve oxidative stress, regulate and control inflammation microenvironment and promote tissue regeneration. The system can efficiently remove MRSA and promote wound repair, provides a new strategy for treatment of drug-resistant bacteria infected wounds, and has a good clinical application prospect.

The application relates to a preparation method and application of a Janus structure photothermal-piezoelectric hydrogel dressing for infected wound repair, and the preparation method comprises the following steps: phenylboronic acid grafted methyl acrylamide gelatin and dopamine grafted hyaluronic acid are respectively dissolved in deionized water, MXene@CeO2 heterojunction is uniformly ultrasonically dispersed, after mixing, sodium hydroxide is used to adjust the pH to be slightly alkaline, and a Janus structure inner layer is formed; phenylboronic acid grafted gelatin and ortho-nitrobenzyl alcohol grafted hyaluronic acid are respectively dissolved in deionized water, BaTiO3 is uniformly ultrasonically dispersed, and a Janus structure outer layer is formed after mixing. The Janus structure photothermal-piezoelectric hydrogel dressing prepared by the application has the characteristics of space-time release to adapt to the wound repair process, in addition, has excellent photothermal conversion performance, kills bacteria on the infected wound through photothermal heating, effectively simulates physiological electrical signals through piezoelectric effect, regulates cell behavior, and can be used for infected wound repair.

The invention relates to a preparation method and antibacterial application of an adjustable manganese-based Prussian blue analogue. Through metal ion doping and lattice engineering regulation and control, a manganese element is introduced into a Prussian blue frame structure, and controllable regulation of the doping amount and optimization of a crystal structure are realized. The prepared material has excellent photothermal conversion capability and multienzyme simulation activity, and shows a photothermal-enzyme synergistic enhancement effect under the irradiation of near-infrared light. In-vitro studies show that the material can efficiently inhibit the growth of gram-negative bacteria; animal experiments further verify that the composition has a remarkable curative effect in infected wound repair. The invention not only provides a preparation method of a nano material with controllable metal doping, but also provides a novel photo-enzyme synergistic treatment method for drug-resistant bacterium infection treatment.

The present invention relates to hydrogels suitable for medical use—especially in wound treatment. The hydrogels contain a metal complex as active ingredient and display excellent antimicrobial action against biofilms. Furthermore, the hydrogels assure an excellent release of moisture and create optimal prerequisites for a rapid healing process even in the case of infected wounds. Further aspects of the invention relate to a process for producing the hydrogels and to wound dressings that contain the hydrogel as a constituent.

The present application relates to the technical field of wound dressing, and particularly relates to a chitosan modified clay-based aerogel wound dressing and a preparation method thereof. The preparation method comprises the following steps: S1: adding clay minerals into deionized water to prepare a slurry, and adding acetic acid while stirring to obtain a clay mineral slurry; S2: dissolving chitosan in an acetic acid solution to prepare a chitosan sol; S3: continuously stirring and mixing the clay mineral slurry and the chitosan sol to obtain a sol; S4: immersing the sol in a metal cation solution first, and then immersing the sol in an anion salt solution to obtain a standing product; and S5: freeze-drying the standing product to obtain a composite aerogel. The present application successfully constructs a three-dimensional porous aerogel dressing, which not only retains the strong hemostatic ability of clay, but also significantly enhances the mechanical strength and antibacterial performance, and exhibits great potential in the treatment of high-exudation complex wounds such as diabetic foot ulcers and infected wounds.

This invention discloses a glycosylated carbon dot functionalized fiber-gel composite dressing and its applications, belonging to the fields of functional materials and biomedicine. First, this invention develops a novel glycosylated carbon dot CS-ACD using a one-step hydrothermal method. This CS-ACD exhibits significantly enhanced antibacterial and anti-inflammatory activity, primarily killing bacteria through a membrane-dissolving mechanism. It also promotes the transformation of macrophages from the pro-inflammatory M1 type to the anti-inflammatory M2 type, thereby reducing the expression and secretion of inflammatory cytokines. Furthermore, this invention combines electrospun nanofibers loaded with this CS-ACD with an in-situ sprayable fibrin gel to prepare an innovative "sandwich" structured fiber-gel dressing. In an experimental mouse model of infected wounds, this dressing effectively reduces bacterial load, regulates the immune response, alleviates wound inflammation, and promotes collagen deposition and angiogenesis, ultimately accelerating wound healing and tissue repair.

This invention belongs to the field of biomedical materials technology, specifically disclosing a core-shell microneedle, a core-shell microneedle patch, its preparation method, and its application. Through structural design and carrier material optimization, this invention provides a core-shell microneedle with a programmed sequential release of tannic acid in the outer shell and verteporfen in the core layer, successfully matching the dynamic physiological rhythm of wound healing and ensuring effective wound repair. In vitro and in vivo biological evaluations have confirmed that the core-shell microneedle patch prepared using the core-shell microneedles of this invention possesses excellent antibacterial, antioxidant, and immune microenvironment-regulating abilities. It can effectively induce macrophage polarization towards the M2 type, specifically inhibit YAP protein expression, and significantly accelerate the closure process of infected wounds. From both upstream immune regulation and downstream fibrosis inhibition levels, it dually blocks the pathological scar formation pathway, ultimately achieving high-quality, scar-free regenerative repair of infected wounds.

The invention discloses growth factor loaded GelMA microspheres for promoting healing of infected wounds and a preparation method and application thereof. The preparation method comprises the following steps: enabling a mixed solution containing methacrylic anhydride gelatin and growth factors to flow into liquid nitrogen, and performing ultraviolet radiation to form microspheres; immersing the obtained microspheres into a mixed solution containing dopamine hydrochloride, zinc sulfate and 2-methylimidazole, and vibrating to obtain the drug-loaded microspheres for promoting healing of infected wounds. According to the microsphere, an outer layer structure formed by crosslinking 2-methylimidazole and Zn < 2 + > is introduced to the surface of a GelMA microsphere, so that the microsphere is endowed with antibacterial performance and a drug sustained-release function. In addition, the outer layer structure can load vascular endothelial growth factors (VEGF), and wound healing is further promoted. And by adding dopamine hydrochloride, the adhesive force of the surface of the microsphere is obviously enhanced, so that the microsphere can be better adhered to wound tissues, and the drug delivery efficiency is improved. The microspheres are particularly suitable for the fields of wound healing and tissue repair.

The application discloses a konjac glucomannan microneedle patch for diabetic wound healing and a preparation method and application thereof, and belongs to the technical field of medical device preparation. The microneedle patch is composed of a needle tip array and a backing layer, wherein the needle tip array and the backing layer are both formed by an oxidized konjac glucomannan and a mixed aqueous solution of hyaluronic acid, and a CuGA-MOF material is added to the aqueous solution for making the microneedle patch. The microneedle patch provided by the application can treat diabetic wounds in a minimally invasive manner. In vitro and in vivo verification shows that the konjac glucomannan microneedle patch loaded with the CuGA-MOF can inhibit bacterial growth, remove active oxygen, regulate macrophage M2 polarization and promote angiogenesis, and can effectively realize rapid healing of diabetic wounds. In addition, the raw material of the microneedle patch is abundant, the preparation method is simple, the cost is low, the safety is high, and the microneedle patch has a wide application prospect in the field of treating infected wounds and chronic wounds.

The invention discloses a multifunctional efficient hemostatic sponge and a preparation method and application thereof, and belongs to the technical field of biomedical materials. The hemostatic sponge is prepared by compounding magnesium aluminum silicate loaded with a polyphenol compound with a gelatin matrix, crosslinking and freeze-drying. The polyphenol compound is selected from one or more of tannic acid, gallic acid, EGCG (epigallocatechin gallate) and quercetin. The preparation method comprises the following steps: dispersing magnesium aluminum silicate into colloid; loading a polyphenol compound on the magnesium aluminum silicate to obtain a composite material; uniformly dispersing the composite material in a gelatin solution, and adding a cross-linking agent for cross-linking; and finally, freeze-drying and forming. Synergistic interaction is achieved through the rapid water absorption and coagulation promoting characteristics of the magnesium aluminum silicate, the physical support effect of the gelatin and the long-acting antibacterial and anti-inflammatory functions of the polyphenol compound, and the obtained sponge has a porous structure, excellent hemostatic performance, lasting antibacterial activity, good biocompatibility and degradability, and can be used for preparing the medical hemostatic sponge. The device is suitable for surgical operation, wound first aid and infected wound surface treatment.

This invention provides an antibacterial and repair-promoting piezoelectric film, its preparation method, and its application, belonging to the field of piezoelectric material technology. The method involves in-situ self-assembly of Fe-MOF on the surface of BTO, followed by further loading with DHA to synthesize an electrically controlled drug-release, antibacterial, and repair-promoting nanocomposite, BTO / Fe-MOF / DHA. BTO / Fe-MOF / DHA is then added to the surface of semi-cured PDMS to form an antibacterial and repair-promoting piezoelectric film, PDMS-BTO / Fe-MOF / DHA. When the PDMS-BTO / Fe-MOF / DHA piezoelectric film is subjected to ultrasonic stimulation, BTO converts mechanical energy into electrical energy, releasing Fe from the Fe-MOF... 3+ Reduced to Fe 2+ Fe-MOF degrades and releases DHA, and ultimately, Fe... 2+ Reacting with synchronously released DHA, it produces a large amount of toxic ROS, which damages proteins and nucleic acids, inducing bacterial death. The antibacterial and repair-promoting piezoelectric membrane of the present invention generates a piezoelectric potential during mechanical deformation caused by animal movement and induces EF at the wound site to promote wound healing. This antibacterial and repair-promoting piezoelectric membrane shows great potential in the future treatment of bacterial infected wounds.

The invention relates to a material in the field of medical supplies, in particular to an easy-to-desorb wound dressing with controllable adhesion, near-infrared light response and antioxidant and antibacterial properties and a preparation method of the easy-to-desorb wound dressing. The wound dressing is of a double-layer integrated hydrogel structure, the upper layer is a photo-thermal hydrogel layer with near-infrared light response and constant-temperature photo-thermal performance, and the wound dressing is formed by compounding and curing core-shell microspheres containing spironolactone photo-thermal molecules, GelMA and PEGDA; the lower layer is a controllable adhesion hydrogel layer based on lipoic acid and is formed by curing components such as LA / LANa, GelMA and NIPAm. The wound dressing can achieve stable constant-temperature photo-thermal antibacterial treatment under irradiation of near-infrared light, meanwhile, the adhesion strength of the wound dressing can be regulated and controlled through temperature, the wound dressing can be firmly attached to a wound surface in the using process, mild and low-damage desorption is achieved during replacement, and the wound dressing has good antibacterial performance, oxidation resistance and biocompatibility and is suitable for being used in the field of medical treatment. The device is suitable for safe treatment and nursing of infected wounds.

The application provides a kind of injectable responsive hydrogel loaded with chitosan oligosaccharide-gallic acid octyl ester and its preparation method and application, and the preparation method comprises: chitosan oligosaccharide-gallic acid octyl ester conjugate is added into carboxymethyl chitosan solution to obtain a mixed solution;The mixed solution and the oxidized tannic acid solution are mixed to form an injectable responsive hydrogel.The three-dimensional hydrogel network formed by carboxymethyl chitosan and oxidized tannic acid is constructed, chitosan oligosaccharide-gallic acid octyl ester is stably loaded in the gel system, can form a physical barrier in the local wound and provide sustained support, thereby significantly improving the retention capacity of antibacterial components in the wound site, prolonging its effective action time, reducing the need for repeated administration.Effectively solve the problem that antibacterial functional materials are difficult to stay in humid infected wounds for a long time and are easy to lose with exudate, can be applied and prepared into antibacterial drugs.

The invention belongs to the technical field of diabetic foot flushing, and particularly relates to a diabetic foot infected wound flushing device which comprises a collecting tank, the collecting tank is hollow, the hollow portion of the collecting tank is communicated with an external negative pressure machine through an air pipe, and the hollow portion of the collecting tank is fixedly communicated with a binding bag through an air pipe; the binding bag is fixedly communicated with a connecting pipe, the top end of the connecting pipe is fixedly communicated with an infusion pipe, the top end of the infusion pipe is fixedly communicated with a timing pipe, the top end of the timing pipe is fixedly communicated with a filter, and the top end of the filter is communicated with an infusion bag through the infusion pipe. Stable negative pressure generated by the negative pressure machine in the binding bag is used as core power, full-automatic starting, stopping and circulation of flushing are achieved, and the negative pressure is not only used for extracting flushing waste liquid and providing a negative pressure treatment environment for a wound surface, but also can drive a timing switch of the electromagnetic switch and drive the spiral blade to move in the infusion tube.

The invention discloses a multifunctional nano-enzyme with self-driven cascade catalytic activity and a preparation method and application thereof, and relates to the field of diabetes wound repair materials, and the nano-enzyme comprises the following components: copper peroxide nano-dots, a zinc-based metal organic framework and glucose oxidase. According to the invention, a step-by-step synthesis strategy is adopted, copper peroxide nano-dots are uniformly encapsulated in a zinc-based metal organic framework, and then glucose oxidase is immobilized through surface modification, so that the nano-enzyme is constructed. The nano enzyme can realize self-driven triple enzyme cascade reaction through ingenious structural design, and plays a synergistic treatment role in a diabetes infected wound microenvironment. The nano-enzyme can effectively reduce blood sugar, efficiently sterilize, relieve inflammation and promote revascularization, and an innovative and efficient treatment scheme is provided for treatment of diabetes infected wounds.

The invention discloses a hydrogel dressing containing pearl sac liquid as well as a preparation method and application of the hydrogel dressing, and belongs to the technical field of medical dressings. The hydrogel dressing is prepared by taking polyvinyl alcohol (PVA) and chitosan (CS) as gel matrixes and loading Markov pearl sac liquid through an in-situ crosslinking technology. The hydrogel dressing is of a three-dimensional porous network structure and is excellent in water absorption performance, the mechanical property of the hydrogel dressing meets the wound repairing requirement, the bacteriostasis rate of the hydrogel dressing on common burn pathogenic bacteria such as staphylococcus aureus and pseudomonas aeruginosa exceeds 90%, and the hydrogel dressing is free of obvious cytotoxicity. When being applied to burn infection wounds, the medical dressing can effectively inhibit bacterial proliferation of the wounds, relieve inflammatory response, promote angiogenesis and tissue regeneration and remarkably improve the closure rate of the wounds, solves the problems that existing burn dressings are narrow in antibacterial spectrum, limited in repair promoting capacity and the like, provides a safe and effective novel dressing choice for clinical treatment of burn infection, and has wide application prospects. Meanwhile, the effective utilization of the martensitic pearl sac liquid is realized.

The invention discloses a CPAM / GS temperature response type gel-sol conversion hydrogel wound dressing and a preparation method of the CPAM / GS temperature response type gel-sol conversion hydrogel wound dressing. The hydrogel takes a matrix formed by crosslinking gelatin and sodium alginate as a carrier, and a core-shell structure CeO2 (at) PtAu (at) Mn2 (CO) 10 composite nanomaterial is loaded. Under the irradiation of near-infrared light, the hydrogel can realize gel-sol conversion and cooperate with photo-thermal, carbon monoxide gas and nano-enzyme catalytic treatment to effectively remove bacteria, disintegrate biological membranes and promote healing of infected wounds.

The invention discloses a bacteriostasis-related protein as well as a biological material and application thereof. The technical problem to be solved by the invention is how to inhibit bacteria. The invention specifically discloses application of the G enzyme. The application is any one of the following items: A1) application in preparation of a product for promoting wound healing; a2) in preparation of a product for promoting healing of infectious wounds; a3) in preparation of a product for promoting and / or assisting in promoting wound healing; a4) in preparation of a product for enhancing the antibacterial and / or bactericidal ability of antibiotics; a5) application in preparation of a product for enhancing the ability of antibiotics to treat or / and prevent eye infection; a6) in improving the cell activity and / or preparing a product for improving the cell activity. The G enzyme is any one of the following items: B1) the amino acid sequence is a protein of a sequence 1 or 31-442 sites of the sequence 1; b2) a protein which is obtained by substitution and / or deletion and / or addition of amino acid residues of the protein B1), has 80% or more of identity with the protein B1) and has the same activity with the protein B1); b3), a fusion protein obtained by connecting the N terminal or / and the C terminal of B1) or B2) with a protein tag. The G enzyme disclosed by the invention has an obvious antibacterial effect when being used for gram-negative bacteria, and can be used for industrial production.

The invention discloses a compound capable of treating skin wounds and a preparation method and application of the compound, relates to the field of skin wound healing, and aims to solve the problems that in the prior art, photo-thermal treatment cannot be compatible with sterilization and weak tissue damage, and sanshool is unstable and easy to degrade, the adopted technical scheme is that PCM is used for loading sanshool and TIIG NPs. Light energy can be converted into heat energy under the mild photo-thermal condition, then phase change of the phase change material lipidosome is promoted, sanshool is released, physical sterilization and chemical sterilization effects are achieved at the same time, the obvious antibacterial effect on staphylococcus aureus and escherichia coli in vitro is achieved, macrophages can be promoted to be polarized to anti-inflammatory M2 phenotype, and the anti-inflammatory effect of the phase change material lipidosome is improved. The wound surface inflammation reaction is relieved. And the compound can completely inhibit the growth of bacteria, promote collagen deposition and realize complete healing of infected wounds within 12 days. The compound has efficient antibacterial and tissue repair functions and is good in biocompatibility.

The invention belongs to the field of biomedical materials, and discloses an antibacterial heterojunction nano-material for repairing diabetes infected wounds and a preparation method and application thereof.The preparation method of the antibacterial heterojunction nano-material comprises the following steps that 1, Ti3C2 nanosheets are dispersed in deionized water, then a chloroauric acid aqueous solution and a BSA aqueous solution are added, stirred and mixed to be uniform, and Ti3C2 nanosheets are obtained; then adding a NaBH4 aqueous solution to carry out in-situ reduction, and after reduction is finished, carrying out centrifugal treatment to obtain a Ti3C2 nanosheet deposited with Au particles; and (2) dispersing the Ti3C2 nanosheets deposited with Au particles in an alcohol solvent, then adding a 4-mercaptophenylboronic acid solution for modification, and after modification is completed, centrifuging, washing and drying to obtain the antibacterial heterojunction nanomaterial. According to the material, the antibacterial activity is enhanced through glucose-triggered material structure transformation cascading, the problem of wound bacterial biofilm breeding is effectively solved, and diabetes infected wound repair is promoted.

The invention belongs to the technical field of biological medicine, and discloses a prodigiosin (PG) and tannic acid (TA) self-assembled carrier-free nano-particle as well as preparation and application thereof. The particles are formed by PG and TA through nano-precipitation self-assembly, and the particle size is 30-50 nm. The preparation method comprises the following steps: respectively dissolving PG and TA in DMSO, mixing and stirring according to a molar ratio of (5-10): 1, dropwise adding ddH2O, carrying out ultrasonic treatment, dialyzing to remove a solvent, centrifuging, taking precipitate, and freeze-drying to obtain powder. The nanoparticles can efficiently kill methicillin-resistant staphylococcus aureus and other multi-drug-resistant bacteria, remove excessive ROS, inhibit TNF-alpha, IL-1beta and IL-6 and promote expression of IL-4, IL-10 and TGF-beta, so that infected skin flap necrosis is relieved, skin repair is promoted, and the nanoparticles have good biological safety and can be used for preparing antibacterial and infected wound treatment drugs.