The invention belongs to the technical field of medicines, relates to disulfiram in-situ-forming gel eye drops as well as preparation and application thereof, in particular to in-situ-forming gel eyedrops which are treated based on a solid dispersion technology and prepared from disulfiram. The disulfiram in-situ-forming gel eye drops are prepared from a main drug, a solid dispersion matrix, an in-situ-forming gel matrix, a bacteriostatic agent, a pH value regulator, a gelation temperature regulator, an osmotic pressure regulator and injection water, wherein the main drug is the disulfiram; the solid dispersion matrix is one or more of HPMC, P407, P188 and PVP; the pH value regulator is borate and has the concentration of 0.001-0.05 percent; and the gel matrix is one or more of the P188 and the P407. The disulfiram in-situ-forming gel eye drops provided by the invention have the efficacy of preventing and treating cataract. According to the preparation, the solid dispersion technologyimproves the solubility and the permeability of indissolvable drugs, and an in-situ-forming gel technology prolongs the corneal retension time of the drugs. The eye drops improve the efficacy of thedisulfiram and have wide development prospect.