139 results about "Oxygenase activity" patented technology

The present invention provides compounds, compositions and methods for inhibiting or reducing reactive oxygen species (ROS) production in cells, such as in cells of the vascular system and in particular the smooth muscle-containing vasculature and / or endothelial cell-containing vasculature and / or adventitial fibroblast-containing vasculature. ROS production may also be inhibited in non-vascular cells of animals including mammals such as humans. Non-vascular cells contemplated herein include nerve cells, stem cells, progenitor cells and some cancer and rumor cells. More particularly, the present invention provides agents and even more particularly, cell-impermeable agents, capable of modulating NADPH oxidase activity, function or levels, thereby controlling superoxide production and production of downstream ROS. The present invention particularly enables agents which are selective against a form of Nox4-containing NADPH oxidase which has a portion of the enzyme such as all or part of the Nox4 component extracellularly exposed.

The invention relates to the molecular cloning and expression of a gibberellin (GA) 20-oxidase gene and its use, for example in transgenic plants. Aspects of the invention include recombinant DNA which encodes a polypeptide exhibiting GA 20-oxidase activity, a recombinant polypeptide exhibiting GA 20-oxidase activity, and transgenic plants which express a GA 20-oxidase gene or reverse GA 20-oxidase sequences.

The invention relates to a method for improving activity of antioxidant enzyme of festuca arundinacea by using fine domestic garbage compost under drought conditions. In the method, a plastic pot with diameter of 7cm and height of 10cm is used through a pot experiment; and the method comprises the steps of: putting 150g of soil into each pot; sowing 0.5g of lawn plants in each pot; uniformly spreading 3g of municipal domestic garbage fine compost with particle sizes of 1,200nm, 600nm and 300nm on the surface of the soil; uniformly covering 20g of soil on the surface of the soil; controlling the temperature to be between 20 and 26 DEG C, wherein the relative humidity is between 40 and 60 percent, and illumination is natural light transmitting indoors; fully watering the plants in the initial stage of sowing, and watering by weighing every day to every two days according to 55 to 65 percent of water-holding capacity in fields after two weeks so as to maintain the capacity within a stress range; and measuring all the indexes on the 44th day. A result shows that the fine compost protects the festuca arundinacea plant from being damaged by active oxygen radicals, and reduces adverse environmental stresses to the plant caused by drought stresses.

The present invention relates, in general, to a biotechnological method for production of (3S,3'S) astaxanthin. In particular, the present invention relates to a peptide having a beta-C-4-oxygenase activity; a DNA segment coding for this peptide; an RNA segments coding for this peptide; a recombinant DNA molecule comprising a vector and the DNA segment; a host cell or organism containing the above described recombinant DNA molecule or DNA segment; and to a method of biotechnologically producing (3S,3'S) astaxanthin or a food additive containing (3S,3'S) astaxanthin, using the host.

The invention pertains to the technical field of biologic degrading for chemical pesticide, and relates to a bacterial strain that can efficiently degrade Pyrethroid, Organophosphorus and vermectins pesticides and the application of the bacterial strain. The bacterial strain is an Acinetobacter sp. JCX22D bacterial strain, the biologic fature of the bacterial strain is Gram's negative, free from flagella, in form of short rod, is of esterase and lipase activity, free from oxidase activity, and can take use glucose and fructose. The bacterial strain is resistant to Ampicillin and low-concentration Spectinomycin and nalectin, and not resistant to rifamide. The degrading efficiency of the bacterial straine is more than 72% for high-efficiency cypermethrin, cyhalothrin, decamethrim, bifenthrin pesticid, is more than 70% for diazinon, parathion-methyl, phoxime and chlorpyrifos, and is 90% for vermectins. The bacterial strain can be used for biologically degrading above pesticides, and biologically recovering or cleaning any water body, soil and agricultural products that are polluted by pesticides.

The invention relates to the molecular cloning and expression of a gibberellin (GA) 20-oxidase gene and its use, for example in transgenic plants. Aspects of the invention include recombinant DNA which encodes a polypeptide exhibiting GA 20-oxidase activity, a recombinant polypeptide exhibiting GA 20-oxidase activity, and transgenic plants which express a GA 20-oxidase gene or reverse GA 20-oxidase sequences.

The invention relates to a nano-packaging material for keeping fruit fresh and the application thereof, pertaining to the technical field of storage and processing of agricultural products. 25 percent to 35 percent of nano-powder (35 percent of nano-silver, 40 percent of nano-titanium dioxide and 25 percent of kaolinite), 50 percent to 60 percent of plastic, 10 percent to 15 percent of coupling agent and 4 percent to 10 percent of wax are taken, evenly mixed in a high speed for 0.5 h to 1.5 h, then kneaded in a plastic inhaling machine and finally extruded and cooled for 1min to 2min to be granulated to obtain the master batches. 30kg to 40kg of plastic particle and 1kg to 2kg of master batches are then fully mixed for 0.5h to 1h to be blown into films so as to obtain the nano-packaging material. When being used for packaging fruit, the nano-packaging material can inhibit the activity of oxidases of fruit and vegetables and postpone the accelerating maturity and senescence to maintain the freshness of the fruit. In addition, the gas exchange of the film is improved and the water-holding property of the package is enhanced to effectively prevent the water loss of the fruit and show an excellent bacteriostatic action. Therefore, the nano-packaging material of the invention has high feasibility and broad market prospect.

The invention discloses sargassum pallidum polysaccharide selenium as well as a preparation method and an application thereof. The preparation method mainly comprises steps as follows: raw material pretreatment, hot water extraction, decoloration, deproteinization, freeze drying and selenylation reaction, wherein the selenylation reaction is implemented as follows: sargassum pallidum polysaccharide and dilute nitric acid are mixed and stirred at the room temperature, sodium selenite and barium chloride are added respectively, the mixture is heated to 60-80 DEG C, stirred and mixed uniformly toreact at the microwave power of 100-500 W for 3-5 min, a sargassum pallidum polysaccharide selenium solution is obtained, cooled to the room temperature, placed in a dialysis bag for dialysis and freeze-dried, and sargassum pallidum polysaccharide selenium is obtained. The preparation method is simple, the reaction condition is mild, the sargassum pallidum polysaccharide selenium is pollution-free and high in selenylation degree, has remarkable alpha-glucosidase inhibition activity, has the activity superior to that of acarbose and can remarkably improve the antioxidase activity of cells andreduce the content of malondialdehyde, a dietary supplement with antioxidant and blood sugar reducing efficacy can be developed, and the sargassum pallidum polysaccharide selenium can be applied to the field of food or medicines.

The invention relates to preparation of protamine gold nanoclusters and application in analogue enzyme color comparison and fluorescence detection. The protamine gold nanoclusters not only have activity of horseradish peroxidase and oxidase, but also have fluorescent characteristic. Hereby, a protamine gold nanocluster analogue enzyme visual color comparator and a fluorescence sensor are established respectively and are used for detecting heavy metal ions. The protamine gold nanoclusters are prepared simply and conveniently, are low in cost, and have excellent fluorescence stability and chemical stability, and the shortcomings that fluorescence of traditional dyestuff is short in service life, photobleaching occurs easily and the like can be overcome; a fluorescence method is high in sensitivity and good in stability, and expensive instruments are not required; and analogue enzyme catalytic color comparison is sensitive and environment-friendly, and can be used for field real-time monitoring. The protamine gold nanoclusters are expected to be expanded and applied to detection and analysis of related substances in the fields of environment and biomedicines and disease markers, and have wide application prospect.

The invention discloses a snapper breeding method. The snapper breeding method includes steps of 1) putting a net cage; 2) throwing fry into the net cage; 3) feeding submersible minced fillet, lemon and tea leaves when fry is smaller than 10 cm long; feeding fish and shrimp, lemon and tea leaves when fry is longer than or equal to 10 cm long; 4) separating fry once every twelve to eighteen days when fry is smaller than 10 cm long and once every fifteen to twenty five days and replacing the net cage with meshes of 15 to 20 mm when fry is 10 to 15 cm long and once every thirty to forty days and replacing the net cage with meshes of 20 to 25 mm when fry is longer than 15 cm. Lemon and tea leaves are fed in the feeding process, so as to effectively suppress lipoxidase activity and reduce aldehydes, and fishy smell of snappers is greatly reduced.

The invention provides a transition metal monatomic nano-enzyme as well as a preparation method and an application thereof, and belongs to the field of catalysts. The transition metal monatomic nano-enzyme is obtained by carrying out heat treatment on a transition metal-doped metal organic framework material, and the transition metal-doped metal organic framework material is prepared by taking water as a solvent. The transition metal monatomic nano-enzyme prepared by the method has uniform morphology, specific surface area and pore diameter, has atomic-scale dispersion active sites, and provides a platform for further research of a metal active center and a catalytic mechanism of a monatomic catalyst. The experimental results show that the transition metal monatomic nano-enzyme prepared by the invention has good mimic oxidase (OXD) activity, mimic peroxidase (POD) activity and mimic halogen peroxidase (HPO) activity, can be used for preparing mimic enzyme preparations with high catalytic activity, and has wide application prospects in the fields of antibiosis, anti-tumor, wastewater treatment, immunoblotting analysis and the like.

The present invention relates to a composition for detecting an infinitesimal quantity of β-1,3-glucan, a preparation method thereof and a diagnostic kit detecting β-1,3-glucan. The composition of the present invention shows phenoloxidase activity by β-1,3-glucan in the presence of calcium ions. Using the composition of the present invention, a sample is gathered from a specimen, the composition of the present invention and calcium ions are added to the sample, and β-1,3-glucan is detected by measuring phenoloxidase activity.

The invention discloses the usage of an ocean collagen peptide preparing for making into antioxidant drugs, antioxidant health food, antioxidant food or antioxidant cosmetics. The products of the ocean collagen peptide have the characteristics of easy absorption, rapid absorption, low viscosity, perfect water-solubility and the like. Animal experiments show that the vitality of antioxidant enzymes is markedly strengthened by providing the ocean collagen peptide for a bandicoot, the content of overoxidation lipoid is reduced, and the invention has the effect of oxidation resistance. Clinical experiments also prove that through oral administration of the ocean collagen peptide, the activity of the antioxidant enzymes of human body can be reduced, generation of lipoid peroxide can be reduced, thereby having the function of oxidation resistance, and facial chloasmata and the like can be dispelled.

A method for detecting 2-oxoglutarate oxygenase activity, which method comprises: (i) contacting a 2-oxoglutarate oxygenase and a substrate of the 2-oxoglutarate oxygenase in the presence of 2-oxoglutarate; (ii) adding a derivatisation reagent capable of forming a fluorescent product with 2-oxoglutarate; (iii) detecting the fluorescent product produced by the reaction between the derivatisation reagent and 2-oxoglutarate, if any, thereby detecting 2-oxoglutarate oxgenase activity.

Disclosed are compounds of the general formula (I):TCnD  (I),compositions comprising an effective amount of said compounds either alone or in combination with other chemotherapeutic agents, and methods useful for treating or preventing cancer and for inhibiting tumour tissue growth. These compounds attenuate the oxidative damage associated with increased heme-oxygenase activity and can reduce cell proliferation in transformed cells. In addition, the described compounds and compositions are useful as neuroprotectants and for treating or preventing neurodegenerative disorders and other diseases of the central nervous system.

The invention discloses application of remedied domestic waste composts in adjusting the activities of antioxidant enzymes of lawn plants; wherein, the remedied waste composts refer to compost matrixes obtained by eluting the composts with water based on the weight ratio of 1:1-3; the activities of the antioxidant enzymes comprise superoxide dismutase, peroxide dismutase and catalase. The technical effects of the invention show that the method of planting lawn plants on the remedied waste composts not only can enhance the activities of the protective enzyme systems of the lawn plants but also can improve the capabilities of the lawn plants in resisting unfavorable growth environment obviously, therefore, the technology not only finds a way for scientifically and effectively planting environment-friendly lawns on the matrixes of the eluted compost, but also can achieve the purpose of reducing the environment pollution caused by the domestic waste composts and provide technical support for applying the remedied waste composts to the lawn planting system.

The invention relates to a method for improving antioxidant enzyme activity of ryegrass by adopting fine domestic garbage compost under drought conditions. The method comprises the following steps of: uniformly scattering garbage trace fertilizers with grain diameters of 1,200 nanometers, 600 nanometers and 300 nanometers respectively on the surface of soil, wherein the weight part ratio of the grain diameter to the soil is 1: 60-80; then, sowing 0.5 gram of tall fescue, controlling the temperature to be between 20 and 26 DEG C and relative humidity to be between 40 and 60 percent, and illuminating the tall fescue with indoor natural light; and fully watering the tall fescue at the initial sowing stage, weighing the water in an amount which is 55 to 65 percent of the field holding amount every day or every two days after two weeks to maintain the water in a stress range, and measuring each index on the 44th day. The method provides technical support for applying the fine-grain urban domestic garbage compost to a lawn planting system.

The invention provides a production method for tomato juice beverage prepared with an enzymatic method, and belongs to the food biotechnology field. The production method has the technical key comprising the following steps of blanching to peel off tomato skins with water of 80-100DEG C; adding color fixative compounded by sodium erythorbate and tea polyphenol of which the mass ratio is 1:2-1:4; keeping the temperature of 65-85DEG C, and carrying out pulping processing; passivating oxidase activity; controlling the oxidation of tomato pulp to keep the natural color of the tomato pulp; carrying out composite enzymatic hydrolysis on the tomato pulp by pectinase NCB-PE40 and cellulase AE80 of which the mass ratio is 1:1-1:3, and filtering to obtain tomato juice; adding 0.10-0.50% of compound stabilizer (which is compounded by 40-50% of xanthan gum, 20-30% of agar powder and 20-40% of pectin) to improve the suspension stability of the tomato juice. Sugar and citric acid are added for blending to obtain the tomato juice beverage with bright color and abundant nutrition.

The invention discloses a compound feed for crimson snappers. The compound feed comprises a fish and shrimp feed, lemons and tea leaves, and the weight ratio of the fish shrimp feed to the lemons and the tea leaves is 100:0.5-1.5:3-5; preferably, the weight ratio of the fish shrimp feed to the lemons and the tea leaves is 100:1:4. A certain number of lemons and tea leaves are added to the compound feed disclosed by the invention on the basis of a conventional feed, so that the activity of lipoxygenase can be effectively inhibited, the generation of aldehydes materials is reduced, and the stink odor of the crimson snappers is largely reduced; because nutritional ingredients in the lemons and the tea leaves generate a certain attractant effect on the crimson snappers, the immunity of the crimson snappers is improved, and the feed coefficients and the survival rate are increased.

Disclosed is a mutant fructosyl amino acid oxidase modified at an amino acid residue involved in proton relay system. The mutant fructosyl amino acid oxidase has reduced oxidase activity while substantially maintaining its dehydrogenase activity. The invention also provides an assay device and assay method for measuring glycated protein.

The invention relates to the technical field of optical sensing, and particularly discloses a cerium-based metal organic framework for Cr (VI) detection, a preparation method and application. The cerium-based metal organic framework employs a cerium dioxide nano-rod with mimic enzyme activity as a template and provides cerium ions as an ion center; then terephthalic acid is added for nucleation and growth to obtain the cerium-based metal organic framework. The cerium dioxide nano-rod is obtained by mixing cerium nitrate hexahydrate and sodium hydroxide for reaction and performing centrifugation, washing and vacuum drying; and the cerium-based metal organic framework is applied to detection of Cr (VI). The cerium-based metal organic framework is synthesized by applying a sacrificial template method for the first time, has higher oxidase activity and selectivity than those of a CeO2 nano material, achieves sensitive detection on Cr (VI), has the advantages of high sensitivity, good selectivity, simplicity and rapidness, and has a good application prospect.

The invention discloses a mesoporous manganese oxide nano-enzyme with a spherical yolk-eggshell structure, and a preparation method and application thereof. Manganese-tannic acid-formaldehyde polymer spheres are roasted in an air atmosphere, so that the mesoporous manganese oxide nano-enzyme with the spherical yolk-eggshell structure is obtained. The mesoporous manganese oxide nano-enzyme is uniform in particle size distribution (70 nm), and has a high specific surface area (127 m<2> / g), a mesoporous structure (the aperture is 5.8 nm) and the yolk-eggshell structure. The mesoporous manganese oxide nano-enzyme with the spherical yolk-eggshell structure has oxidase-like activity and can catalytically oxidize 3, 3', 5, 5'-tetramethyl benzidine (TMB) to generate a color reaction, and the Michaelis constant (Km) of the material with TMB as a substrate is 97 mmol / L. The material also has oxidability, can be used for colorimetric detection of reduced biomolecules (such as glutathione, cysteine and ascorbic acid), and has good selectivity.

The invention discloses an application of eutrophic water in the regulation of antioxidase activity of lawn plants, wherein the eutrophic water is the eutrophic water with 25 percent-75 percent of the original concentration; and the eutrophic water has TN of 9.7 mg / L-13.1mg / L, TP of 0.6mg / L -0.9mg / L and pH value of 8.12-8.99, wherein the activity of antioxidase comprises of superoxide dismutase, peroxide dismutase and catalase. Results show that the diluted eutrophic water is applied to irrigation to reinforce the activity of a protective enzyme system of lawn plants, thereby being capable of obviously improving the resistance ability of the lawn plants to adverse growing environment and providing technical support for the application of the eutrophic water in a lawn plant establishment system.

The invention discloses an auxiliary identification method of wheat with a high kernel lipoxidase activity character and special primers thereof. The method comprises the following steps of: simultaneously carrying out PCR (Polymerase Chain Reaction) amplification by using a primer pair A and a primer pair B by taking gene group DNA of wheat to be tested as a template; and if DNA segments of 235 bp and 117bp are obtained, judging the wheat to be tested is candidate wheat with the high kernel lipoxidase activity character, wherein the high kernel lipoxidase activity character means the lipoxidase activity of a kernel is above 75 A234min-1g-1, the primer pair A refers to a primer pair consisting of DNA shown as sequences 1 and 2 in a sequence table, and the primer pair B refers to a primer pair consisting of DNA shown as sequences 3 and 4 in the sequence table. The method is simple, easy, stable and reliable and can carry out molecular marker assisted selection on wheat lipoxidase gene LOX to culture high-quality wheat breed with high lipoxidase activity and can test the lipoxidase activity in wheat. The method and the special primers are about to play a very important role in wheat lipoxidase activity character detection and wheat breading.

The invention relates to a nano-enzyme as well as a preparation method and application thereof. The nano-enzyme comprises a bovine serum albumin skeleton and gold nanoparticles attached to the bovineserum albumin skeleton. The nano-enzyme involved in the invention is a multifunctional nano-enzyme, has both oxidase activity and peroxidase activity, has potential application value in the fields ofreducing substance detection, enzyme-linked immunosorbent assay and the like, and has a remarkable antibacterial effect due to the fact that various free radicals can be produced.

The present invention disclosed a method to detect pregnancy and identify pseudopregnancy, pregnancy and pregnancy loss using ceruloplasmin as a non-invasive marker in animal urine samples. More specifically, this invention provide provides a method to use a non-invasive marker of inflammation, the acute phase protein ceruloplasmin, in urine samples to detect pregnancy and to distinguish between pregnancy and pseudopregnancy in mammals. The steps of this method include: collecting fresh urine sample from a mammal, adding a ceruloplasmin substrate to the urine sample, measuring oxidase activity of said ceruloplasmin through a sequential reading of absorbance of a photometric product using a spectrophotometer to determine the concentration of ceruloplasmin in the at least one urine sample.

The invention relates to an application method for applying angleworms on waste composting lawns to regulate the antioxidase of plants on the lawn. The invention has the technical proposal that a certain proportion of angleworms are put into a waste composting lawn to regulate the antioxidase activity of the plants on the lawn, thereby improving the growth environment of the lawn. The examination proves that the quantity of on-ground biology and under-ground biology is increased through the movement of angleworms; the MDA content is decreased at the same time; and the activity of SOD and POD is decreased, thereby proving that adding the angleworms with particular proportion on the waste composting lawns can decrease the harm to the plants from the poor environment, thereby promoting the growth of the lawns.

The invention relates to a synthetic oligopeptide containing three aminophenols; cysteine is in the middle of a sequence, one end of the sequence is glutamic acid or glycin, and the other end of the sequence is aminophenol proline or Gamma-propalanine having antiretroviral function. The function of the synthetic oligopeptide is similar to that of glutathione, i.e., removing 1, 1-DPPH free radicals under in-vitro condition, improving the oxidation resistance of anabaena and tobacco cells, and the activity of oxidizing enzymes under salt stress, and improving the heat resistance of wheat seedlings.

The invention relates to a method for enhancing immunity of Fenneropenaeus chinensis. The method is characterized in that Fenneropenaeus chinensis is fed with single Fenneropenaeus chinensis basal feed and Fenneropenaeus chinensis basal feed mixed with 1.4% (by weight) of hydrolyzed fish protein powder alternately, i.e. Fenneropenaeus chinensis is firstly fed with Fenneropenaeus chinensis basal feed mixed with 1.4% (by weight) of hydrolyzed fish protein powder for 7 days, and then fed with single Fenneropenaeus chinensis basal feed for 7 days, over and over again. The measured serum acid phosphatase activity, serum phenol oxidase activity, serum lysozyme activity, peroxidase activity change and serum superoxide dismutase activity of Fenneropenaeus chinensis fed by using the method provided by the invention are not significantly different from those of a Fenneropenaeus chinensis group continuously fed with hydrolyzed fish protein powder and a Fenneropenaeus chinensis group continuously fed with peptidoglycans; and the survival rate and weight increase rate are not significantly different from those of the Fenneropenaeus chinensis group continuously fed with hydrolyzed fish protein powder and the Fenneropenaeus chinensis group continuously fed with peptidoglycans. Therefore, compared with the method in which Fenneropenaeus chinensis is continuously fed with Fenneropenaeus chinensis basal feed mixed with 1.4% (by weight) of hydrolyzed fish protein powder, the method provided by the invention can obtain the same immunity effect but can reduce half of economic cost.