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Transition metal monatomic nano-enzyme as well as preparation method and application thereof

A technology of transition metals and transition metal salts, applied in the fields of botany equipment and methods, chemical instruments and methods, catalyst activation/preparation, etc., can solve complex methods, unfavorable acquisition of single-atom catalysts, and difficult comparison of catalytic activity of nano-enzyme preparations and other issues to achieve the effect of high catalytic activity

Active Publication Date: 2021-10-01
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current nano-enzyme preparations are still in their infancy, and there are mainly the following problems: First, due to the different chemical properties of different metal elements, it is difficult to obtain nano-enzyme preparations with similar structures, sizes and coordination environments. The catalytic activities of nano-enzyme preparations with different metal centers were further compared to reveal the key role played by metal centers in the enzymatic catalysis process; Industrialized production; the 3rd, the kind of metal element in the nanometer imitation enzyme preparation reported at present is mainly Fe element, relatively single
However, the applicant of the present invention has found that the preparation method reported in the literature is sensitive to doped transition metals, and different types and contents of transition metals will lead to serious changes in the morphology and size of the resulting product, which is not conducive to obtaining products with similar coordination environments. single atom catalyst

Method used

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  • Transition metal monatomic nano-enzyme as well as preparation method and application thereof
  • Transition metal monatomic nano-enzyme as well as preparation method and application thereof
  • Transition metal monatomic nano-enzyme as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Preparation of Fe single atom nanozyme:

[0046] 1, preparation of transition metal doped ZIF-8 precursor

[0047] Put Zn (NO 3 ) 2 · 6h 2 O (0.3626g), iron nitrate (iron nitrate and zn (no 3 ) 2 · 6h 2 The molar ratio of O was 1: 40) and hexadalkyltrimel bromide (0.0175 g) were dissolved in 18 ml of ultra-pure water, and mixed uniformly formed uniform, transparent solution A;

[0048] 2-methylimidazole (5.6752 g) was dissolved in 82 ml of ultra-pure water to obtain a uniform, transparent solution B;

[0049] The solution A was rapidly added to the solution B, and the two were mixed uniform, and the reaction was given at room temperature, and then cured, and the resulting precipitation was washed three times with water and ethanol (1: 1), vacuum dried to give a transition metal Doped ZIF-8 precursor.

[0050] 2, preparation of transition metal monoimer nanozyme

[0051] Weigh the transition metal doped ZIF-8 precursor and placed in a porcelain boat, and then place...

Embodiment 2

[0052] Example 2 Preparation of the rest of the transition metal monoimer nanozyme

[0053] Referring to the preparation method of Example 1, the difference is only to replace the nitric acid iron in step 1 to each transition metal salt or transition metalic acid shown in Table 1, and the furnace temperature in step 2 is set to each furnace shown in Table 1. Temperature, prepared a corresponding transition metal monoimer nanozyme.

[0054] Table 1 Transition metal salts or transition metal acids used in each transition metal monogenic nanozyme, furnace temperature

[0055]

[0056] The beneficial effects prove below the experimental examples.

experiment example 1

[0057] Experimental Example 1 Micro-morphology

[0058] 1, experimental method

[0059] The transition metal-doped ZiF-8 precursor, transition metal monoimer nanozyme prepared in Example 2 was taken, and the transition metal monogenic nano enzyme was thermally treated at 900 ° C furnace temperature. Get the sample.

[0060] 2, experimental results

[0061] The morphological characterization of each transition metal doped ziF-8 precursor is like Figure 8 As shown, it can be seen that each transition metal doped ZIF-8 precursor has a uniform topography and size.

[0062] The morphological characterization of each transition metal monogenmotonininase figure 1 As shown, it can be seen that the present invention produces each transition metal monogenmase is a nanoparticle having a uniform cubic structure with a size of 20-200 nm, each transition metal monogenic nanoase has a uniform morphology and size. Micro-morphological characterization results of each transition metal monogenmase f...

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Abstract

The invention provides a transition metal monatomic nano-enzyme as well as a preparation method and an application thereof, and belongs to the field of catalysts. The transition metal monatomic nano-enzyme is obtained by carrying out heat treatment on a transition metal-doped metal organic framework material, and the transition metal-doped metal organic framework material is prepared by taking water as a solvent. The transition metal monatomic nano-enzyme prepared by the method has uniform morphology, specific surface area and pore diameter, has atomic-scale dispersion active sites, and provides a platform for further research of a metal active center and a catalytic mechanism of a monatomic catalyst. The experimental results show that the transition metal monatomic nano-enzyme prepared by the invention has good mimic oxidase (OXD) activity, mimic peroxidase (POD) activity and mimic halogen peroxidase (HPO) activity, can be used for preparing mimic enzyme preparations with high catalytic activity, and has wide application prospects in the fields of antibiosis, anti-tumor, wastewater treatment, immunoblotting analysis and the like.

Description

Technical field [0001] The present invention belongs to the field of catalysts, and more particularly to a transition metal monogenic nanozyme and a preparation method thereof. Background technique [0002] The enzyme plays an important role in nature, but its complex preparation process, strict use, storage conditions, and high prices limits the use of enzymes. In order to overcome these shortcomings, develop corresponding imitation enzyme preparations into research hotspots, the Nobel Chemical Award in 2018 granted Frances H.arnold to commend her outstanding work in biological enzyme-oriented modification. The traditional enzyme engineering focuses on the structural design of macromolecules, although it can effectively improve the synthetic performance of the enzyme, expand the use occasions of the enzyme, but the preparation process is very challenging. Therefore, it is urgent to develop a simple enzyme preparation for prepared methods. [0003] Since 2007, Yan Xiyun topic gro...

Claims

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Application Information

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IPC IPC(8): B01J23/10B01J23/22B01J23/26B01J23/28B01J23/30B01J23/34B01J23/36B01J23/60B01J23/66B01J23/80B01J35/10B01J37/03B01J37/08C02F1/72A61P35/00A01N59/16A01N59/20A01P1/00A01P3/00
CPCB01J23/80B01J23/22B01J23/26B01J23/34B01J23/28B01J23/60B01J23/30B01J23/36B01J23/66B01J23/10B01J37/031B01J37/086B01J37/088A61P35/00C02F1/722C02F1/725A01N59/16A01N59/20B01J35/393B01J35/23B01J35/615B01J35/617B01J35/643
Inventor 曹素娇程冲邱逦李玲郑懿娟朱笔挥马田马朗李爽陈帆
Owner SICHUAN UNIV
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