63 results about "Parathion methyl" patented technology

The invention pertains to the technical field of biologic degrading for chemical pesticide, and relates to a bacterial strain that can efficiently degrade Pyrethroid, Organophosphorus and vermectins pesticides and the application of the bacterial strain. The bacterial strain is an Acinetobacter sp. JCX22D bacterial strain, the biologic fature of the bacterial strain is Gram's negative, free from flagella, in form of short rod, is of esterase and lipase activity, free from oxidase activity, and can take use glucose and fructose. The bacterial strain is resistant to Ampicillin and low-concentration Spectinomycin and nalectin, and not resistant to rifamide. The degrading efficiency of the bacterial straine is more than 72% for high-efficiency cypermethrin, cyhalothrin, decamethrim, bifenthrin pesticid, is more than 70% for diazinon, parathion-methyl, phoxime and chlorpyrifos, and is 90% for vermectins. The bacterial strain can be used for biologically degrading above pesticides, and biologically recovering or cleaning any water body, soil and agricultural products that are polluted by pesticides.

The invention relates to a method for detecting ten types of organophosphorus pesticides by using magnetic separation-gas chromatography. The method is used for quickly detecting ten types of organophosphorus pesticides (dipterex, methamidophos, acephate, omethoate, moncrotophos, dimethoate, phosphamidon, parathion-methyl, malathion and chlorpyrifos) based on combination of solid-phase extraction, magnetic separation and the gas chromatography. The method comprises the following steps: preparing a magnetic solid-phase extraction agent, establishing a combination system of the magnetic separation and the gas chromatography, and detecting the organophosphorus pesticides in objects to be detected. A method capable of quickly separating and detecting ten types of organophosphorus pesticides at high sensitivity simultaneously is established by taking prepared magnetic chitosan microspheres with high absorption performance on the ten types of organophosphorus pesticides such as dipterex and the like as adsorption functional materials and using combination of the solid-phase extraction, magnetic separation and the gas chromatography. The method is simple in experimental operation, high in detection sensitivity, low in cost and applicable to detection of residues of ten types of organophosphorus pesticides such as trace dipterex and the like in all products.

The invention relates to a multiresidue fast immune-gold localization test strip for eight organophosphorus pesticides such as parathion-methyl. The test strip comprises a lining plate, a sample pad, a gold localization combination pad, a cellulose membrane and a water absorption pad, wherein the gold localization combination pad is a glass cellulose membrane for adsorbing a gold-labeled antibody; a linear hidden detection line printed with a carrier protein solution coupled with the parathion-methyl is arranged on the cellulose membrane; and the linear hidden detection line and a linear hidden contrast line printed by rabbit antimouse IgG are parallel to each other. The test strip has the characteristics of wide detection spectrum, high sensitivity, quickness, simplicity, intuitiveness and accuracy; pre-treatment is simple; any instrument equipment is not needed; and operation of professionals is not required.

The invention provides a preparation method of an organic framework material containing zinc metal. The method comprises steps as follows: porphyrin, (4-carboxyphenyl)benzene, zinc nitrate and N,N-dimethylformamide are mixed under the ultrasonic condition, and a mixed solution is obtained; the mixed solution is subjected to a hydrothermal reaction, and the organic framework material containing zinc metal is obtained. The organic framework material containing zinc metal is prepared from raw materials including porphyrin, (4-carboxyphenyl)benzene and zinc nitrate, the preparation method is simple, few steps are used, fluorescent signals of the organic framework material containing zinc metal can be quenched by parathion-methyl, the fluorescence quenching degree is linearly correlated with logarithms of the content of parathion-methyl, the organic framework material containing zinc metal is used for detecting parathion-methyl, so that parathion-methyl can be rapidly, simply and sensitively detected.

The invention discloses a silver nanoparticle modified carbon nanotube-egg membrane composite surface enhanced Raman scattering (SERS) substrate as well as a preparation method and application thereof. The SERS substrate is composed of a carbon nanotube loaded on an egg membrane and silver nanoparticles modified on the carbon nanotube, wherein the carbon nanotube is 0.5-2mu m long and is 30-50nm in diameter, and sizes of the silver nanoparticles are 5-10nm. The preparation method comprises the following steps: dissolving egg shell in an acetic acid solution to obtain an egg membrane, ultrasonically processing the egg membrane in an ammonium solution to obtain alkalified egg membrane, then dispersing a carbon nanotube processed by carboxylation in de-ionized water and ultrasonically processing to obtain a carbon nanotube solution, and ultrasonically processing the alkalified egg membrane in the carbon nanotube solution to obtain the egg membrane loaded with the carbon nanotube on the surface; and finally, sputtering silver on the egg membrane in a sputtering instrument to obtain a target product. The SERS substrate disclosed by the invention can serve as an active substrate for surface enhanced Raman scattering; and through a laser Raman spectrometer, content of rhodamine or parathion-methyl or polychlorinated biphenyl PCB-3 or bovine serum albumin attached on the substrate is measured.

The invention relates to organic phospholipid hydrolase, a mutant, an encoding gene and an application thereof in degradation of organophosphorus pesticides. The organic phospholipid hydrolase PoOPH is originated from edible oil pseudomonadaceae, the mutant of which is a protein which is derived from the protein shown as an amino acid sequence SEQ ID No.1 in a sequence table and has the activity of the organic phospholipid hydrolase, wherein the 250th and the 263rd amino acids are substituted by a single site or double sites. Compared with a parent, the catalytic activities of the PoOPH mutant to parathion-methyl and paraoxon-ethyl are respectively improved by 5680 and 37.5 folds. Meanwhile, the PoOPH mutant maintains the excellent heat stability of the parent. The PoOPH mutant provided by the invention has two attributes at the same time: high vitality and excellent heat stability and has a good application prospect in the field of degrading organophosphorus pesticides.

The invention discloses a film assembled from gold nanoparticle, as well as a preparation method and application of the film. A plurality of layers of the gold nanoparticles are mutually adhered on a conducting substrate so as to form the film of which the thickness is 200nm-2<mu>m, wherein the grain diameter of the gold nanoparticles is 30-120nm; and the clearance or gap between every two gold nanoparticles is equal to or smaller than 10nm. The method comprises the following steps of: sputtering a gold film on the surface of the conducting substrate so as to obtain the conducting substrate of which the surface is covered with the gold film; placing the conducting substrate in gold electrolyte by using a graphite flake as anode and using the conducting substrate of which the surface is covered with the gold film as a cathode; and performing electro-deposition under constant current to obtain the target product. The film is high in SERS activity, and is extremely easy to widely and commercially apply to rapid trace detection for parathion-methyl of pesticides.

The invention relates to a Fe-based 3 o 4 / CNTs Synergistic Microwave Degradation of Organic Pesticides. The technical scheme adopted is: in the water containing organic pesticide, add catalyzer, irradiate 1-7min under microwave 150-750W; Described catalyzer is Fe 3 o 4 Supported carbon nanotube nanoparticles Fe 3 o 4 / CNTs. The organic pesticide is Diuron or methyl parathion. As a new method for treating organic pesticide wastewater, the invention has the advantages of high degradation efficiency, fast speed, low cost and no secondary pollution, etc., is suitable for large-scale organic pesticide wastewater treatment, and can quickly and completely degrade organic pesticide in a short time.

The invention discloses a parathion-methyl detection method which comprises the following steps: preparing a n-doped carbon quantum dot (N-CDs) by using a hydrothermal method; decomposing parathion-methyl under an alkali condition to generate paranitrophenol; mixing the prepared N-CDs with a hydrolysis product, namely the paranitrophenol; and enabling the paranitrophenol to absorb fluorescence ofthe N-CDs to quench the fluorescence of the N-CDs, and detecting the concentration of the parathion-methyl according to fluorescence intensity. In the method, sample pretreatment is avoided, large-size instrument or enzyme is not needed, the detection method is simple to operate, high in detection specificity, free of interference of other common pesticides, high in detection sensitivity, low in detection limit, high in stability and wide in application, and can be applied to detection on parathion-methyl in fruits, vegetables, river samples, and the like, and the cost can be greatly reduced.The method has good application prospects and potential application values in the fields of detection and analysis.

The invention discloses a preparation method and application of a parathion-methyl artificial hapten, a parathion-methyl artificial antigen and a parathion-methyl specific antibody, and belongs to the technical field of pesticide immunologic chemistry. The method comprises the following steps of: introducing spacer arms and active groups into a methoxyl site of parathion-methyl by taking 3-mercaptopropionic acid as a functional group reagent to synthesize O-methyl-N-(2-carboxyethyl) sulfo-O-(4-nitrobenzophenone) phosphorothioate (MP for short) serving as the hapten; and coupling the hapten with protein in a mixed anhydride method and an active ester method to prepare the artificial antigen. In the method, the parathion-methyl antibody with high affinity and high specificity is prepared by immunizing an animal through an immunogen, and enzyme-linked immunosorbent assay (ELISA) established by the antibody can be used for detecting trace quantity of parathion-methyl residues in a sample quickly.

The invention discloses a parathion-methyl extraction and detection method based on an N-octylpyridinium hexafluorophosphate electrode. The method comprises the following steps: immersing the N-octylpyridinium hexafluorophosphate electrode into a sample solution, and directly extracting and enriching parathion-methyl; after the extraction and enrichment are finished, with the N-octylpyridinium hexafluorophosphate electrode as a working electrode, a saturated Ag / AgCl electrode as a reference electrode and a platinum filament as an auxiliary electrode, detecting the parathion-methyl through an electrochemical cyclic voltammetry or a linear scanning method. According to the method, the sample pretreatment is greatly simplified, and the pesticide is directly extracted, concentrated and detected on the electrode. Furthermore, the whole electrode is formed by a uniform mixture, the structure is firm, and film falling problem is avoided. In addition, a new surface can be obtained by slightly polishing the electrode with weighting paper, so that measurement can be performed.

The invention relates to the field of environmental protection, in particular to degradation of organic waste by biomaterials and / or photocatalytic materials. A microbial strain is selected from one or more of bacillus subtilis with the collection number of CGMCC NO.1.9083 preferably, bacillus megaterium with the collection number of CGMCC NO.1.16094 preferably and aspergillus sydowii with the collection number of CGMCC NO.3.13937 preferably. A photocatalyst, namely, nano titania, is used to cooperate with microbes, so that the degradation agent has very good effects on degrading methamidophos, parathion-methyl and triazophos.

The invention relates to an organophosphorus pesticide degradation enzyme obtained through genetic engineering reconstruction. A random mutation reconstructed organophosphorus pesticide degradation enzyme is generated by the fact that three amino acid loci of an organophosphorus pesticide degradation enzyme are derived from pseudomonas stutzeri. The replaced amino acid loci are a 31st locus, a 124th locus and a 246th locus. The organophosphorus pesticide degradation enzyme is good in degradation activity for chlorpyrifos and parathion-methyl, and also has good heat stability.

The invention provides a manganese dioxide and sulfite system. The manganese dioxide and sulfite system comprises nano manganese dioxide and sulfite, and the mass ratio of the sulfite to the nano manganese dioxide is 0.25: 1-10: 1. The invention further provides a preparation method of the manganese dioxide and sulfite system. The preparation method comprises the following steps: preparing the nano manganese dioxide; and mixing the sulfite with the nano manganese dioxide according to the mass ratio of 0.25: 1-10: 1 to obtain the manganese dioxide and sulfite system. By the provided manganese dioxide and sulfite system, organic pollutants in soil and polluted water can be removed. By the provided manganese dioxide and sulfite system, hydrolysis and oxidative degradation rate of organophosphorus pesticide in the water is increased effectively, a purpose of efficiently removing the organophosphorus pesticide in the water is achieved, and the degradation rate of parathion-methyl within 30h reaches 97.1%.

The invention relates to an organophosphorus pesticide degrading enzyme obtained through gene engineering in a transformation mode. The organophosphorus pesticide degrading enzyme transformed through random mutation is generated through replacing three amino acid locuses of organophosphorus pesticide degrading enzyme derived from pseudomonas stutzeri. The amino acid replacing locuses replace a seventh locus, a one hundred and twenty-fourth locus and a two hundred and forty-sixth locus. The organophosphorus pesticide degrading enzyme not only is excellent in activity degrading of chlorpyrifos parathion-methyl, but also has excellent heat stability.

There is provided a molecularly imprinted polymer (MIP) sensor for sensing a hydrophobic target molecule, comprising a MIP film comprising a hydrophobic polymer host, such as polyvinylidene difluoride (PVDF) or polystyrene (PS), with one or more binding sites for one or more target molecules, such as parathion methyl (PTM); and a sensing substrate, such as mass sensitive quartz crystal microbalance (QCM). The MIP film is coated on a surface of a sensing substrate. There is also provided a method of making the MIP sensor and a method for detecting / quantifying a target molecule using the MIP sensor.

The invention discloses a multifunctional lubricating oil compound additive, which comprises a first antioxidant antiwear agent, a second antioxidant antiwear agent, a first antioxidant agent and a second antioxidant. The first antioxidant antiwear agent is selected from non-parathion-methyl type organic multimetallic compound and / or non-parathion-methyl type organic monometal compound, the second antioxidant antiwear agent is selected from organic boric acid ester compound and / or inorganic oil soluble boric acid salt compound, the first antioxidant agent is selected from dialkyl group diphenylamine and / or biphenols compound, and the second antioxidant is beta-(3,5-dule-tertiary butyl-4-hydroxycyclohexyl phenyl ketone) propionic acid isooctanol ester. The multifunctional lubricating oil compound additive contains no parathion-methyl, avoids ternary catalysis poisoning of automobiles, is environmentally friendly, has good antioxidant and anti-friction performance, can effectively restrain increase of oil product viscosity, prolongs service life of oil products, particularly can effectively restrain acidity change of the oil products, and prolongs long-time stability and service life of the oil products.

The invention discloses a method for simultaneously detecting parathion-methyl, chlorpyrifos and trichlorfon based on quantum dot labeled biomimetic fluorescence immunoassay. According to the invention, the multi-recognition-site Fe3O4@SiO2@MIP is used as the common biomimetic antibody, and three organophosphorus pesticide haptens such as parathion-methyl, chlorpyrifos and trichlorfon are respectively labeled with three kinds of quantum dots with different emission wavelengths at the same excitation wavelength, such that the simple and rapid biomimetic fluorescence immunoassay method for simultaneously detecting the three organophosphorus pesticides such as parathion-methyl, chlorpyrifos and trichlorfon is established; and the method has advantages of low cost, simple experiment operation,accurate measurement result and low detection limit, and is suitable for the simultaneous detection of various organophosphorus pesticides in various foods.

The invention provides enterobacter bacteria for efficiently degrading organophosphorus pesticides and a culturing method thereof. The enterobacter bacteria are characterized in that the main biological property of the enterobacter bacteria is Gram-negative, thalli are in a short-rod shape without spores, and the size is 0.6-1.1*1.2-2.5 mum; and the enterobacter bacteria have single-end overgrowing flagella and are aerobic bacteria, the optimum growing temperature is 30-37 DEG C, the optimum pH value is 7-8, the enterobacter bacteria form a bacterial colony with the diameter of 1-2 mm after being cultured on a blood agar medium for 48 h, and the bacterial colony is circular in shape and yellow in color, has no transparence, knurls, trim edge and moist and glossy surface. The Genbank login number of the bacterial strain 16SrDNA is GQ380575. The tolerance concentration of the bacterial strain on parathion-methyl and Dursban can reach 5000 mg / L in an inorganic salt medium containing 1% of cane sugar, the tolerance concentration on dimethoate reaches 10000 mg / L, the tolerance capacity of the bacterial strain is furthest higher than that of agricultural dose used in agricultural production, the degradation rate for the organophosphorus pesticides in soil reaches above 90%, and the enterobacter bacteria solve the problem that pesticide residues in the agricultural production exceed the standard, and can be used for producing green and nuisanceless agricultural products.

The invention relates to a bispecific monoclonai antibody capable of simultaneously recognizing imidacloprid and parathion-methyl pesticides, and a preparation method thereof, belonging to the field of biological technology. The bispecific monoclonai antibody FQ-D6 which can be stably secreted and simultaneously resist imidacloprid and parathion-methyl pesticides is prepared by fusing hybridoma cell capable of secreting anti-parathion-methyl monoclonal antibody and an imidacloprid antigen-immunized rat splenocyte cell through a hydridizatio-hybridoma technique. Proved by effectiveness verification, the antibody can be used for fast and sensitively detecting residuals of the parathion-methyl and the imidacloprid in agricultural production environment and agricultural products. The preparation technology of the bispecific monoclonai antibody capable of simultaneously resisting imidacloprid and parathion-methyl pesticides is simple, convenient and feasible, the whole preparation process of the antibody is not required for special instruments and equipment, thereby being easy for factorization scale production.

The invention discloses a pest repellent and a preparation method thereof and relates to the field of crop protection. The pest repellent comprises, by weight parts, 10-20 parts of methyl-isp, 5-10 parts of parathion-methyl, 2-6 parts of tributyl tin chloride, 7-12 parts of cyclohexanone, 5-8 parts of isobutyl cyanide, 2-4 parts of cyclodextrin, 3-8 parts of methylbenzene, 1-2 parts of carbomer, 2-3 parts of ethanol with a concentration of 95%, 7-9 parts of polyethylene glycol, 0.5-1 part of triethanolamine, 7-10 parts of prickly ash ethanol extract and 4-6 parts of water hyacinth ethanol extract. By adoption of the technical scheme, the method is reasonable in preparation steps and convenient to operate, and the prepared pest repellent is non-toxic and harmless and has a certain promotion effect of plant growth while having high repelling performance.

The invention provides a cobalt nickel bimetal sulfide organophosphorus pesticide biosensor, and a preparation method thereof. The cobalt nickel bimetal sulfide organophosphorus pesticide biosensor comprises a carbon paste electrode and an acetylcholinesterase layer; the surface of the carbon paste electrode is provided with modification layers including a Nafion-NiCo2S4 layer, the acetylcholinesterase layer, and a Nafion layer form interior to exterior successively. A preparation method of NiCo2S4 comprises following steps: a soluble nickel salt and a soluble cobalt salt are dissolved in an ethanediol solution containing a cationic surface active agent, and a cobalt nickel bimetal sulfide is prepared via solvothermal method. The cobalt nickel bimetal sulfide organophosphorus pesticide biosensor possesses excellent enzyme immobilization performance, is high in sensitivity and stability; immobilization of acetylcholinesterase on the surface of the carbon paste electrode is realized so as to construct the cobalt nickel bimetal sulfide organophosphorus pesticide biosensor; and the cobalt nickel bimetal sulfide organophosphorus pesticide biosensor can be applied in detection on parathion-methyl and paraoxon-methyl.

The invention discloses preparation and application for an electrochemical sensor based on a nano-structure boron-doped diamond electrode, in particular relates to a preparation method for a nano-structure boron-doped diamond electrode and a method for the nano-structure boron-doped diamond electrode in rapid detection on organophosphorus pesticide residues, and aims at solving the technical problems of low electrocatalytic activity, difficult surface re-construction, and poor selectivity and sensitivity of a boron-doped diamond (BDD) electrode. The method disclosed by the invention comprises the following steps (1) pre-depositing a layer of gold nanodots on a boron-doped diamond electrode as a mask; (2) etching the morphology of a nano-structure on the boron-doped diamond surface in an Ar / H2 atmosphere; and (3) etching the boron-doped diamond surface, and cooling to obtain the nano-structure boron-doped diamond electrode. According to the preparation and the application disclosed by the invention after the technical scheme is adopted, the performance of the electrode is improved, the sensitivity of the electrode is improved, and the electrode is directly applied to detection on the organophosphorus pesticide residues with electrochemical characteristics, such as parathion and parathion-methyl.

The invention relates to ultralow-volume liquid containing lufenuron and organic phosphorus insecticide, which comprises chemical composition, surfactant and solvent. The chemical composition is composed of lufenuron and an active ingredient II. The active ingredient II can be optional one of fenitrothion, dipterex, parathion-methyl, isazofos, pyridaphenthion, quinalphos, triazophos, colophonate, phoxim, fenitrothion, pirimiphos-methyl, fenthion, cyanophos, profenofos, chlorpyrifos-methyl, prothiofos, sulprofos, dimethoate, malathion, methidathion and isocarbophos. The ultralow-volume liquid is convenient in process, long-acting and highly efficient, targets are less apt to resist the ultralow-volume liquid, environment pollution is low, insecticide and water are economized, and insecticide utilization rate is increased. The ultralow-volume liquid can be used for controlling various pests for rice, wheat, corn, vegetables, tobacco, tree trees, fruit trees and sugarcane by ultralow-low spraying, low-volume spraying or ultralow-volume electrostatic spraying.

The invention discloses a film assembled from gold nanoparticle, as well as a preparation method and application of the film. A plurality of layers of the gold nanoparticles are mutually adhered on a conducting substrate so as to form the film of which the thickness is 200nm-2<mu>m, wherein the grain diameter of the gold nanoparticles is 30-120nm; and the clearance or gap between every two gold nanoparticles is equal to or smaller than 10nm. The method comprises the following steps of: sputtering a gold film on the surface of the conducting substrate so as to obtain the conducting substrate of which the surface is covered with the gold film; placing the conducting substrate in gold electrolyte by using a graphite flake as anode and using the conducting substrate of which the surface is covered with the gold film as a cathode; and performing electro-deposition under constant current to obtain the target product. The film is high in SERS activity, and is extremely easy to widely and commercially apply to rapid trace detection for parathion-methyl of pesticides.

The invention discloses a pest repellent and a preparation method thereof, and relates to the field of crop protection. The pest repellent is prepared from, by weight, 10-20 parts of isofenphos-methyl, 5-10 parts of parathion-methyl, 2-6 parts of tributyl tin chloride, 7-12 parts of cyclohexanone, 5-8 parts of isobutyl cyanide, 2-4 parts of cyclodextrin, 3-8 parts of methylbenzene, 1-2 parts of carbomer, 2-3 parts of 95% ethyl alcohol, 7-9 parts of polyethylene glycol, 0.5-1 part of triethanolamine, 7-10 parts of Chinese prickly ash ethanol extract and 4-6 parts of water hyacinth ethanol extract. According to the technical scheme, preparation steps are reasonable, operation is convenient, and the prepared pest repellent is poisonless and harmless, has high phobotaxis and can promote the growth of plants to some degree.

The invention discloses a pest repellent and a preparation method thereof and relates to the field of crop protection. The pest repellent is prepared from, in parts by weight, 10-20 parts of isofenphos-methyl, 5-10 parts of parathion-methyl, 2-6 parts of tributyl tin chloride, 7-12 parts of cyclohexanone, 5-8 parts of isobutyronitrile, 2-4 parts of cyclodextrin, 3-8 parts of methylbenzene, 1-2 parts of carbomer, 2-3 parts of 95% ethanol, 7-9 parts of polyethylene glycol, 0.5-1 part of triethanolamine, 7-10 parts of a pepper ethanol extract and 4-6 parts of a water hyacinth ethanol extract. With the adoption of the technical scheme, the preparation steps are reasonable and convenient to operate, and the prepared pest repellent is non-toxic and harmless, has high repelling property and can have a certain promotion function on plant growth.

The invention discloses a pest repellent and a preparation method thereof, and relates to the field of crop protection. It is a pest repellent. In parts by weight, the pest repellent comprises 10-20 parts of methyl 5‑10 parts of parathion, 2‑6 parts of tributyltin chloride, 7‑12 parts of cyclohexanone, 5‑8 parts of isobutylcyanide, 2‑4 parts of cyclodextrin, 3‑8 parts of toluene, carba 1-2 parts of pome, 2-3 parts of 95% ethanol, 7-9 parts of polyethylene glycol, 0.5-1 part of triethanolamine, 7-10 parts of ethanol extract of Zanthoxylum bungeanum and 4-6 parts of ethanol extract of water hyacinth, By adopting the technical scheme of the invention, the preparation steps are reasonable and easy to operate, and the prepared pest repellent is non-toxic and harmless, has high repellency and can promote the growth of plants to a certain extent.

The invention discloses a parathion-methyl degradation enzyme mutant as well as an encoding gene and application thereof and belongs to the field of construction and application of the parathion-methyl degradation enzyme mutant. The parathion-methyl degradation enzyme mutant with a secretion capacity, which is remarkably increased in yeast supernatant, is screened from a constructed yeast mutant library by adopting a DNA (Deoxyribonucleic Acid) shuffling technology. The invention further discloses a recombinant expression vector and a recombinant host cell, which contain a parathion-methyl degradation enzyme mutant encoding gene. According to the parathion-methyl degradation enzyme mutant disclosed by the invention, the secretion capacity of the parathion-methyl degradation enzyme mutant in the yeast supernatant is remarkably increased, and industrial production requirements can be met; and the parathion-methyl degradation enzyme mutant has an important application prospect in the aspect of degrading organic phosphorus.