9 results about "Spectinomycin" patented technology

The application provides a spectinomycin-stevioside co-crystal and a preparation method, which is made of spectinomycin and stevioside, and the powder X-ray diffraction pattern of the spectinomycin-stevioside co-crystal has characteristic peaks at diffraction angles 2theta = 9.2°±0.2°, 11.2°±0.2°, 12.1°±0.2°, 12.7°±0.2°, 13.7°±0.2°, 14.6°±0.2°, 16.0°±0.2°, 16.7°±0.2°, 17.0°±0.2°, 19.4°±0.2°, 21.3°±0.2°, 24.5°±0.2°, 25.2°±0.2°, 26.1°±0.2°, 27.1°±0.2°, 27.8°±0.2°, 29.0°±0.2°, 29.6°±0.2°, 33.2°±0.2°. Compared with the existing spectinomycin, the spectinomycin-stevioside co-crystal has better water solubility, and the prepared spectinomycin-stevioside has higher bioavailability.

The application discloses a synergistic bactericidal composition, comprising the following components: a toona sinensis extract: 16-2048 mu g / mL; a macrolide antibiotic: 0.0625-4096 mu g / mL; the bacteria are staphylococcus aureus or methicillin-resistant staphylococcus aureus, and the macrolide antibiotic is erythromycin or azithromycin. The application further discloses a use of the synergistic bactericidal composition in preparation of a medicine for treating skin and soft tissue infections. The composition provided by the application can convert the traditional macrolide antibiotics such as erythromycin and azithromycin which only have a bacteriostatic effect into a preparation which has clear bactericidal activity on staphylococcus aureus and drug-resistant strains thereof. After the toona sinensis extract is combined with the antibiotic, a synergistic effect is exhibited, and the concentration of the toona sinensis extract and the antibiotic required when the toona sinensis extract and the antibiotic are used alone can be greatly reduced.

The invention discloses a multi-immunochromatography test strip with unified detection limit for detecting four aminoglycoside drugs and application of the multi-immunochromatography test strip. The test strip comprises a water absorption plate, a detection pad, a colloidal gold pad, a sample pad and a PVC (polyvinyl chloride) bottom plate, wherein a gold-labeled antibody is fixed on the surface of the colloidal gold pad, and when a sample flows through the colloidal gold pad, the gold-labeled antibody is dissolved and serves as a tracer molecule to migrate along with a liquid phase; four detection areas T1, T2, T3 and T4 and a quality control line area C are arranged on the detection pad, a kanamycin antigen, a gentamicin antigen, a neomycin antigen and a spectinomycin antigen are sprayed on the four detection areas respectively, and a goat anti-mouse antibody is sprayed on the quality control line area C. According to the present invention, the expensive reagent and the base material are not required, the production cost is low, the operation is simple, the professional training is not required, the detection efficiency is high, the detection cost is low, the multiple immunochromatography test strip is used for simultaneously detecting kanamycin, gentamicin, neomycin and spectinomycin, and the detection limits can achieve 5 ng / mL.

The invention relates to an aptamer for specifically detecting neomycin and application of the aptamer, and belongs to the technical field of detection. Firstly, a novel aptamer (the affinity reaches 1.17 + / -0.32 mu M) with good affinity to neomycin is designed, and the aptamer has no reaction to other interfering antibiotics such as chloramphenicol (CAP), oxytetracycline (OTC), enrofloxacin (ENR), azithromycin (AZT), roxithromycin (RXM) and ciprofloxacin (CIP) and has good specificity. Secondly, two fluorescence signal probes of MOF coated FITC and AgNCs are also synthesized, a ratio type fluorescence sensing system is constructed, high-precision and high-sensitivity detection of the target neomycin is realized through a double-signal ratio, and background signal influence caused by environmental interference and fluorescent dye leakage is effectively eliminated.

The application discloses a strain of macrolide antibiotic high-efficiency degradation fungus Trichoderma asperellum TS7-1 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the Trichoderma asperellum TS7-1 is CGMCC No.41372. The strain Trichoderma asperellum TS7-1 (CGMCC No.41372) provided by the application has high-efficiency degradation capacity for various macrolide antibiotics (erythromycin, roxithromycin and azithromycin), and the removal rates of the three antibiotics are all above 85% within 36 hours. The high-concentration antibiotic tolerance, degradation rate and degradation spectrum of the Trichoderma asperellum TS7-1 are obviously superior to those of other strains in the prior art. The Trichoderma asperellum TS7-1 is directly separated from a natural soil body without antibiotic pollution, has no hidden danger of animal and plant pathogenicity, and has no microbial toxicity to degradation products of the macrolide antibiotics, so that secondary pollution is avoided, and excellent environmental safety is achieved.

This invention discloses a method for detecting the bioavailability of antibiotics in soil using continuous solvent extraction-solid phase extraction-liquid chromatography-tandem mass spectrometry. The method first uses continuous solvent extraction to extract the bioactive and extractable antibiotic components from the soil. Then, a solid phase extraction column is used for sample purification. Finally, multiple reaction monitoring (MRM) mode of liquid chromatography-tandem mass spectrometry is used to determine the concentrations of various antibiotics, including chlortetracycline, doxycycline, oxytetracycline, tetracycline, sulfadiazine, sulfadiazine, sulfamethoxazole, ciprofloxacin, enrofloxacin, norfloxacin, ofloxacin, azithromycin, erythromycin, roxithromycin, tylosin, trimethoprim, and lincomycin. This invention combines continuous solvent extraction with solid phase extraction, coupled with liquid chromatography-tandem mass spectrometry detection, to achieve accurate evaluation of trace antibiotic bioavailability under complex matrix conditions.

The invention discloses a synergistic bactericidal composition which comprises the following components: 16 g / mL-2048 g / mL of cortex albiziae extract, 10 g / mL-20 g / mL of plant extract, 10 g / mL-20. The content of the macrolide antibiotics is 0.0625 g / mL to 4096 g / mL; the bacteria are staphylococcus aureus or methicillin-resistant staphylococcus aureus, and the macrolide antibiotics are erythromycin or azithromycin. The invention also discloses application of the synergistic bactericidal composition in preparation of medicines for treating skin and soft tissue infection. According to the composition provided by the invention, macrolide antibiotics, such as erythromycin and azithromycin, which only have a bacteriostatic effect traditionally can be converted into preparations which have definite bactericidal activity on staphylococcus aureus and drug-resistant strains thereof. After the cortex albiziae extract and the antibiotics are combined for use, an extremely strong synergistic effect is shown, and the concentrations required when the cortex albiziae extract and the antibiotics take effect independently can be greatly reduced.

The invention belongs to the technical field of environmental microorganisms, and particularly relates to an aerobic denitrifying bacterium and application thereof, the aerobic denitrifying bacterium is Raoultella sp. 752, is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 22, 2025, has the preservation number of CGMCC No.35338, and has the preservation number of CGMCC No.35338; the strain has the capability of synchronously degrading high-concentration AZM and nitrate, has the advantages of high adaptability, simplicity, feasibility and the like, and can be directly used for denitrification treatment of high-concentration AZM-containing wastewater in pharmacy, culture and the like.

The invention discloses a synergistic bactericidal composition, which comprises the following components: 256g / mL to 8192 g / mL of poplar bud flavin; the content of the macrolide antibiotics is 0.125 g / mL to 4096 g / mL; the bacteria are staphylococcus aureus or methicillin-resistant staphylococcus aureus, and the macrolide antibiotics are erythromycin or azithromycin. The invention also discloses an application of the synergistic bactericidal composition in preparation of a medicine for treating infection caused by staphylococcus aureus or methicillin-resistant staphylococcus aureus. According to the composition provided by the invention, macrolide antibiotics, such as erythromycin and azithromycin, which only have a bacteriostatic effect on staphylococcus aureus and drug-resistant strains thereof traditionally can be converted into preparations with definite bactericidal activity. After the poplatin and the antibiotics are combined for use, a remarkable synergistic effect is shown, and the concentration of the antibiotics required for realizing the sterilization effect can be greatly reduced.