85 results about "Spectinomycin" patented technology

The invention discloses a medicament formula for relieving pain caused by infusion medicament and preventing phlebitis. The medicament formula can be used for relieving pain caused by intravenous supplementing potassium, infusion mannitol, azithromycin, fructose diphosphate sodium and chemotherapeutic medicaments, and can be used for effectively preventing and treating extravasation phlebitis caused by long-term use of a remaining needle, infusion of vein high-nutrition medicaments, infusion of chemotherapeutic medicaments, infusion extravasation and the like. According to the medicament formula, 20-35ng of anisodamine, 6-13ml of lidocaine, 15-26mg of dexamethasone and 4-6ml of glycerin are arranged in a sterile kidney basin, sterile gauze is coated above a puncture part after being uniformly wetted, and an external preservative film is wound on the gauze. The medicinal formula has the advantages of continually and efficiently relieving local pain caused by infusion medicaments, and effectively preventing and treating local inflammation, swelling pain and stripped cable change caused by infusion.

The invention pertains to the technical field of biologic degrading for chemical pesticide, and relates to a bacterial strain that can efficiently degrade Pyrethroid, Organophosphorus and vermectins pesticides and the application of the bacterial strain. The bacterial strain is an Acinetobacter sp. JCX22D bacterial strain, the biologic fature of the bacterial strain is Gram's negative, free from flagella, in form of short rod, is of esterase and lipase activity, free from oxidase activity, and can take use glucose and fructose. The bacterial strain is resistant to Ampicillin and low-concentration Spectinomycin and nalectin, and not resistant to rifamide. The degrading efficiency of the bacterial straine is more than 72% for high-efficiency cypermethrin, cyhalothrin, decamethrim, bifenthrin pesticid, is more than 70% for diazinon, parathion-methyl, phoxime and chlorpyrifos, and is 90% for vermectins. The bacterial strain can be used for biologically degrading above pesticides, and biologically recovering or cleaning any water body, soil and agricultural products that are polluted by pesticides.

The present invention is directed to a pharmaceutical composition comprising a therapeutically effective amount of misoprostol and an effective amount of an antibiotic. A suitable antibiotic is selected from the group consisting of doxycycline, gentamicin, tobramicin, ciprofloxacin, clindamycin, clarithromycin, azithromycin and metronidazole. Preferred antibiotics are doxycycline and ciprofloxacin. More preferably, the antibiotic is doxycycline. In its second aspect, the present invention is directed to a method for treating periodontal disease in a mammalian patient comprising administering to a mammalian patient in need of such treatment a therapeutically effective amount of misoprostol and an effective amount of an antibiotic. Typically, the mammalian patient is a human.

The invention discloses a novel frozen semen diluent used for improving the freeze-thaw quality and fertilization rate of frozen bovine semen and a preparation method thereof. Every 100ml of the prepared frozen semen diluent contains 0.9-1.2g of sodium citrate, 3-5g of sucrose, 18-22ml of yelk, 4-7ml of glycerin, 6-9mg of vitamin E, 500-700mg of vitamin C, 35mg of spectinomycin and 80 thousand units of gentamicin. The invention adds VE and VC into the semen diluent for the first time, so as to increase the motility rate and acrosome integrity of the frozen sperm as well as the activity of antioxidant enzyme in seminal plasma, thus improving the quality and fertilization rate of the freeze-thaw sperm.

The invention relates to a culture medium for fermentation production of spectinomycin through streptomyces spectabilis and a fermentation method. A primary seed culture medium, a secondary seed culture medium and a fermentation culture medium all contain corn oil, maltose, beer yeast dry residues and earthworm meal. According to the invention, maltose replaces glucose, beer yeast dry residues and earthworm meal replace fish meal, peptone, soybean cake meal and corn steep liquor, and the culture medium formula is optimized, so that the problem of high costs of raw materials is solved, the environmental effect on the source of the raw materials is reduced to the utmost extent, sufficient supply of the raw materials is guaranteed, and stable and efficient production of spectinomycin is realized. Meanwhile, the culture medium can improve the fermentation unit and shorten the fermentation period.

The invention relates to a spectinomycin colloidal gold detection card. According to the method, a sample liquid absorbing part, a colloidal gold labeling part, a detection reaction part and a water absorbing part are orderly stuck on a backing of test paper. The detection reaction part is coated by an antigen 1 bar which is applied to detection and is used as a detection line, and at the same time is coated by an IgG1 bar which resists the second species of animal protein and is used as a reference line. The rapid detection test paper bar has high specificity, can carry out semi quantitative detection, can be used at the environmental temperature of 4 to 40 DEG C, and is suitable for an individual farmer, a food hygiene and quality inspection department, customs and other animal source food to carry out rapid detection on spectinomycin residues. The spectinomycin colloidal gold detection card provided by the invention has the advantages of high specificity, high sensitivity, simple and convenient operation and the like, and can carry out semi quantitative detection.

The invention discloses azithromycin gel eye drops and a preparation process thereof. The eye drops are prepared from a main medicine, namely azithromycin and excipients such as an adhesive, a gel matrix, an isotonic regulator, a preservative, an antioxidant, a buffering agent and the like. The adhesive, namely polycarbophil in the eye drops can increase the biological adhesion of the eye drops, so that the detention time of medicines in eyes is further prolonged. The invention provides a practical, convenient and reliable ophthalmic preparation for treating bacterial conjunctivitis, and solves the problems that the detention time of medicines in an eye drop formulation in the eyes is short, the medicines are not easy to absorb, the bioavailability is low and the like.

The invention discloses a measuring method for detecting residual quantities of streptomycin, dihydrostreptomycin, kanamycin, spectinomycin and neomycin in food simultaneously. The method comprises steps as follows: (1) extraction; (2) purification; (3) preparation of a matrix standard working solution; (4) measurement with LC-MS / MS (liquid chromatography-tandem mass spectrometry). The method has the advantages of simplicity in operation, accuracy, high sensitivity and good repeatability, can completely meet technical requirements for corresponding product security detection in China, the European Union, America and Japan and can provide powerful technical support for guaranteeing food security of people in China and sound development of export trades.

The invention discloses a preparation method and an application of a sensor for simultaneously detecting four aminoglycoside antibiotics and belongs to the technical field of novel function materials and biosensor analysis. According to the invention, a reference electrode is printed by the use of a silver paste and four working electrodes and a counter electrode are printed by the use of a conductive carbon paste on a rectangular substrate surface coated with a layer of a polyester film. Thus, a four-channel screen-printed electrode is prepared. The preparation method is characterized in that antibodies of four aminoglycoside antibiotics including streptomycin, gentamycin, kanamycins and spectinomycin are respectively fixed on surfaces of the four working electrodes by the use of a Pt nanoparticle-sulfated graphene-carboxymethyl chitosan conductive composite nano-material; and after the detection liquids are dripped, the four working electrodes are connected with a multichannel electrochemical workstation detection circuit so as to realize simultaneous detection of the four aminoglycoside antibiotics. The sensor provided by the invention is simple to prepare and convenient to process, requires low cost, and is convenient to carry. The detection method is simple and rapid and has high sensitivity and good specificity.

The invention discloses a spectinomycin high-producing strain, a screening method of the strain and an application of the strain in the production of spectinomycin. The spectinomycin high-producing strain disclosed by the invention is named streptomycesspectablies DC15-01, is preserved in China general microbiological culture collection center (CGMCC) with the collection number CGMCC NO.6221, and the collection date is 14th, June, 2012. The spectinomycin high-producing strain screening method provided by the invention can fast screen the high-producing strain and is easy to implement; furthermore, the fermentation yield of the spectinomycin of the DC15-01 strain provided by the invention is improved by 30.6%.

The invention provides a novel azithromycin preparation and particularly provides an azithromycin dry-suspended granule with odor masked. The dry-suspended granule is composed of a coated micro-pellet and a taste-corrective auxiliary material composition. The coated micro-pellet is composed of a drug-carrier pellet, an isolation layer and an odor-masking layer, wherein the odor-masking layer is mainly prepared from Eudragit L30D-55. The taste-corrective auxiliary material composition is mainly composed of sorbitol and isomaltitol. Through the special method, bitter taste and odor of the azithromycin medicine are masked, so that compliance is improved, and meanwhile, with the special coating layer, the preparation is improved in stability without influence on release degree of the azithromycin.

The present invention relates to an antibiotic resistance profile for Neisseria gonorrhoeae by assessing the presence of mutations (e.g., SNP) in antibiotic resistant genes that confer bacterial resistance against antibiotics such as penicillin, tetracycline, fluoroquinolones, cephalosporin, macrolides and spectinomycin. There is provided a method and a kit for generating an antibiotic resistance profile for Neisseria gonorrhoeae by utilizing a multiplex PCR to amplify segments of antibiotic-resistant genes, allele-specific primer extension to detect gene mutation, and detection of such gene mutations with gel electrophoresis, capillary electrophoresis, or DNA microarray. The present method provides useful information to physicians relating the antibiotic susceptibility of Neisseria gonorrhoeae against different classes of antibiotics. Relying on this personalized diagnostic tool, physicians can better inform about antibiotic susceptibility and thereby open up medical intervention avenues for treating Neisseria gonorrhoeae with antibiotics. There is provided a therapeutic application of the antibiotic resistance profile that has advantages of: (i) providing a more effective regime for gonorrhea treatment; and (ii) halting the evolutionary pressures towards antibiotic resistance in the Neisseria gonorrhoeae therapy.

The invention discloses a method for simultaneously analyzing various aminoglycoside compound residues in agricultural and animal products. The method comprises the following steps: weighing a sample;adding an ammonium acetate buffering solution; after carrying out oscillation and extraction, carrying out centrifuging; separating an extracting solution obtained by centrifuging and regulating to asuitable range by utilizing a PH (Potential of Hydrogen) meter; purifying by adopting dispersed solid-phase extraction and a solid-phase extraction small column. Ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS), electrospray ionization (ESI) and multi-reaction monitoring (MRM) scanning modes are adopted. Compared with other similar methods, the method disclosed by the invention has the advantages of simplicity in operation and high sample flux; eight types of aminoglycoside compounds including streptomycin, dihydrostreptomycin, spectinomycin, gentamicin, apramycin, kanamycin, amikacin and neomycin are detected at the same time; the detection efficiency is greatly improved, and rapid qualitative and quantitative detection can be rapidly carried out; the detection limit can completely meet domestic and overseas laws and regulations and limitation requirements, and powerful support is provided for food safety guarantees and import and export of the agricultural and animal products of China.

The invention provides high resolution X-ray crystal structures of the 30S ribosome, obtained from Thermus thermophilus 30S subunit, having a tetragonal space group P41212 to which are bound an antibiotic selected from the group paromomycin, streptomycin, spectinomycin, tetracycline, pactamycin and hygromycin B. An advantageous feature of the structure is that it diffracts at about 3 Å resolution. The invention also provides a crystal of 30S having the three dimensional atomic coordinates of the 30S ribosome, the coordinates being provided in any one of tables 1 to 4. The data may be used for the rational design and modelling of inhibitors for the 30S ribosome, which have potential use as antibiotics.

The invention discloses a spectinomycin, streptomycin, gentamycin and neomycin detection test strip and a method thereof. The test strip comprises micro-porous strips, micro-porous reagents, a test paper and micro-porous plugs, the micro-porous reagents are lyophilized colloidal gold labeled spectinomycin, streptomycin, gentamycin and neomycin specific antibodies respectively, the test paper is formed by sequentially connecting a sample absorption pad, a reaction film, a water absorption pad, a protection film and a substrate, the reaction film comprises four detection lines and a quality control line, the four detection lines are coated with a spectinomycin-carrier protein conjugate, a streptomycin-carrier protein conjugate, a gentamycin-carrier protein conjugate and a neomycin-carrier protein conjugate respectively, and the quality control line is coated with an antiantibody. The method for detecting the spectinomycin, streptomycin, gentamycin and neomycin by using the test strip has the advantages of simplicity, rapidness, perceptual intuition, accuracy, portability, wide application range, low cost, and easy popularized use.

The invention belongs to the technical field of medical dressings for repairing burn skin wounds, and provides a double-sustained-release drug-loaded hydrogel dressing with double-layer microspheres entrapped by a semi-interpenetrating network. Acrylic acid is used as a pH-sensitive monomer. The pH / temperature dual-sensitive intelligent hydrogel takes methacrylic acid 2-ethyl ester and oligomeric (ethylene glycol) methyl ether methacrylate as temperature-sensitive monomers, and an anti-inflammatory drug gentamicin sulfate is entrapped; the preparation method comprises the following steps: preparing a calcium alginate core sphere entrapped with bovine serum albumin through a high-voltage electrostatic instillation method, coating the surface of the core sphere with a chitosan shell layer entrapped with an antibacterial drug azithromycin to form a double-layer microsphere, and loading the double-layer microsphere into the hydrogel to obtain the hydrogel dressing capable of precisely and slowly releasing two drugs and one protein. According to the pH / temperature change of a wound surface microenvironment, the hydrogel can achieve accurate controlled release of drugs and improve the utilization rate of the drugs, and is a novel double-sustained-release drug-loaded hydrogel dressing with a semi-interpenetrating network combined double-layer microsphere structure and good comprehensive performance.

The invention provides lincomycin-spectinomycin compound nano-emulsion, and belongs to the technical field of pharmacy. The compound nano-emulsion is prepared from the following components in parts by weight: 1-20 parts of lincomycin hydrochloride, 1-25 parts of spectinomycin, 15-40 parts of surfactant, 1-25 parts of co-surfactant, 1-16 parts of oil and 18-75 parts of water. The compound nano-emulsion is a novel preparation which is combined with characteristics of lincomycin hydrochloride and lincomycin hydrochloride, and has the advantages of uniform grain diameter distribution, good fluidity, high stability, good permeability and good bioavailability; the compound nano-emulsion can be quickly phagocytosed by reticuloendothelial cells after being taken orally, drugs can quickly take effects, and constant plasma concentration and pharmacological effect can be maintained, the bioavailability can be improved, drug effect can be enhanced, and using amount and taking times of the medicine can be reduced.

The invention discloses a dilute solution used for preserving racoon dog seminal fluid at normal temperature, and a semen collection method. The dilute solution comprises following ingredients: 36 to 38g of glucose, 1.20 to 1.30g of sodium bicarbonate, 1.20 to 1.30g of ethylene diamine tetraacetic acid, 5.50 to 6.30g of sodium citrate, 0.65 to 0.85g of potassium chloride, 0.50 to 3.05g of bovine serum albumin, 0.05 to 0.30g of acetylcysteine, 0.50 to 1.20g of penicillin, 0.30 to 1.00g of streptomycin, 0.05 to 1.50g of lincomycin and 0.01 to 0.05g of spectinomycin; and a mixture of the ingredients above is diluted to 1000ml by adding triple-distilled water so as to obtain the dilute solution. Key steps of the semen collection method are that: female racoon dogs in heat are used as dummy teaser; conditioned reflex of male racoon dogs to artificial semen collection is formed by training; the training is repeated for 3 to 4 times until conditioned reflex is formed; and then the female racoon dogs are replaced by a female racoon dog-simulated dummy teaser which is filled with urine of female racoon dogs in heat so as to collect male racoon dog semen. The dilute solution and the semen collection method can be used for collecting racoon dog semen, diluting for preservation, realizing artificial insemination and obtaining offspring; and are capable of promoting the popularization of racoon dog artificial insemination technologies, and increasing economic benefits of racoon dog industry.

The invention provides an azithromycinl actobioate hydrate and the purpose. The formula of the azithromycinl actobioate hydrate of the invention is C38H72N2O12question mark 2C12H22O12question mark nH2O, in which n is between 2.0 to 5.5.the hydrate has good water solubility, high storage stability, and is applicable to treat and prevent human or animal infectious diseases caused by Gram positive or negative bacteria.

This invention relates to an eye use transmission system of macrolide type antibiotic hyaluronic acid natrium. The goal of this invention is to provide a macrolide type antibiotic eye use medicine preparation with carrier is hyaluronic acid or its salt. It is used to cure germ eye region infection. The macrolide type antibiotic in this invention includes antibiotic that has 14 yuan,15yuan, 16yuan large ring construction features , for example, natural antibiotic bring by streptomycete erythromycin, josamycin, spiramycin, medemycin etc. and semisynthesis derivant from by structure modification of natural antibiotic, such as roxithromycin, azithromycin, clarithromycin, rokitamycin, telithromycin.

The invention discloses a feed additive used for preventing and treating vibrio gill-rot disease of aquatic animals and a preparation method thereof. The feed additive used for preventing and treating the vibrio gill-rot disease is prepared from the following raw materials in parts by weight: 15-25 parts of agastache rugosus, 10-20 parts of coptis chinensis, 7-15 parts of gentian, 3-7 parts of common andrographis herb, 3-7 parts of rheum officinale, 3-7 parts of pulsatilla chinensis, 1-3 parts of spectinomycin and 4-6 parts of lincomycin. The feed additive used for preventing and treating thevibrio gill-rot disease can be used for improving the body immunity and antiviral capacity of the aquatic animals, lowering the infection probability of gill-rot disease bacterial virus and improvingthe prevention and treatment effect of the gill-rot disease, is convenient to administrate in a water drinking mode, high in absorption rate, wide in distribution and wide in safe range, and has no medicament residue and no toxic and side effects.

Belonging to the technical field of enzyme genetic engineering, the invention relates to a Cre / lox system based method for construction of resistance gene free chromosome integrated recombinant bacillus subtilis expressing D-psicose 3-epimerase. The method includes: taking integration vector pDGIEF as the starting vector, deleting the original spectinomycin (spc) antibiotics resistance gene, and introducing a new antibiotics resistance gene fragment of lox71-spc-lox66 to obtain plasmid pDGI-7S6; fusing a P43 promoter at the upstream of the DPE enzyme gene of ATCC 35704 to construct P43-DPE, constructing the P43-DPE into pDGI-7S6 to obtain pDGI-7S6-P43-DPE; then converting 1A751 competence, under the action of amylase homologous arm, integrating P43-DPE and lox71-spc-lox66 to 1A751 chromosome; utilizing the Cre / lox system to knock out the spc resistance gene, thus obtaining a food grade safe 1A751 / lox-DPE strain producing D-psicose 3-epimerase. The strain is named as Bacillus subtilis SK38.002 with a preservation number of CCTCC NO:M 2015258, has fermentation broth total enzyme activity up to 6U / mL, and has important industrial application value.

The invention discloses a freezing diluent formula for improving the quality of Holstein bull frozen-thawed X-spermatozoa. The freezing diluent comprises the following ingredients per 100 ml: 0.9-1.2 g of sodium citrate, 3-6 g of sucrose, 16-18 mg of low density lipoprotein, 4-7 ml of glycerol, 500-700 mg of Vitamin E, 100-200 mg of Vitamin B12, 0.35 mg of spectinomycin, 70000-80000 International Unit (IU) of gentamicin and the balance of distilled water for making up 100ml. The experimental data show that the freezing diluent improves the activity and the acrosome intact rate of frozen-thawed X-spermatozoa, and improves the quality of frozen-thawed spermatozoa.

The invention discloses a method for detecting the aminoglycoside antibiotic residue amount in an animal-derived food and particularly relates to a method for detecting the aminoglycoside antibiotic residue amount in the animal-derived food based on HILIC chromatographic column tandem triple quadrupole mass spectrometry. The method is characterized in that a sample is subjected to extraction, solid phase extraction and ZIC-HILIC chromatographic column separation and then is determined through quadrupole mass spectrometry. The detected neomycin, spectinomycin, dihydrostreptomycin, streptomycin and gentamicin concentrations have good liner relation within the range of 10-500 micro-g / kg, a response value S / N under the concentration of 20 micro-g / kg is good and is greater than 10, the sample truth degree has no obvious matrix effect, and the detection limit of the concentration in the sample can be reduced to be 100 micro-g / kg. Compared with the prior art, the method has the advantages of being short in sample earlier-stage treatment time, low in cost, simple, convenient, comprehensive in matrix coverage, high in accuracy and the like.

The invention discloses an azithromycin microsphere medicament comprising azithromycin and acceptable carrier material on the pharmacy, which adopts biodegradable gelatin as the carrier material. A gelatin microsphere is prepared through emulsification coagulation, wherein the azithromycin is enwrapped in the gelatin microsphere, thereby diminishing the granularity of the medicament, improving the bioavailability of the medicament, concealing effectively the bitter of the medicament, having stable physicochemical property and good reproducibility and especially facilitating infants to take medicine. The preparation technology of the invention has the advantages of stability and feasibility, low cost and easy reproducibility, which can create giant economic benefit and can be extended as beneficial reference of other medicament preparation technology in the pharmacy industry.

The invention discloses compound levofloxacin hydrochloride soluble powder for treating respiratory diseases of poultry. The soluble powder is prepared by uniformly mixing the following components in percentage by weight: 2-10 percent of levofloxacin hydrochloride, 2-10 percent of azithromycin, 2-6 percent of venenum bufonis, 10-30 percent of liquorice extract, 3-9 percent of Kameiding, 1-5 percent of trimethoprim lactate, and the balance of pharmaceutically applicable auxiliaries. The compound preparation is compounded by latest market medicines, traditional Chinese medicinal extracts, novel antiasthmatic drugs and medicinal synergistic agents, and fatal factors are comprehensively taken, so that the compound levofloxacin hydrochloride soluble powder has the effects of anti-inflammation, sterilization, anti-virus, heart strengthening, cough relieving and allergic reaction resistance, can be used for improving the anti-disease immunity of poultry, repairing injured mucous membrane and effectively controlling secondary infection, and has an effective treatment effect to respiratory diseases of poultry.

The invention discloses a test strip and a method for detecting spectinomycin. The test strip comprises a micropore strip, a micropore reagent, test paper and a micropore plug, wherein the micropore reagent is a spectinomycin specific antibody marked by freeze-dried colloidal gold, and the spectinomycin specific antibody is a monoclonal antibody; and the test paper is composed of a sample absorption cushion, a reaction film, a water absorption cushion, a protection film and a bottom plate which are connected in order, the reaction film comprises a strip of detection line print and a strip of quality control line print, the detection line is coated with spectinomycin-carrier protein conjugate, and the quality control line is coated with an antiantibody. The method for detecting spectinomycin by the test strip has advantages of simpleness, rapidness, perceptual intuition, accuracy, convenience for carrying, wide suitable range, low cost, and easy popularization and usage.

The invention provides an azithromycin lactobionate hydrate and the preparation and application thereof. The molecular formula of the azithromycin lactobionate hydrate is C38H72N2O12.2C12H22O12.nH2O, wherein n is equal to between 2.0 and 5.5, the hydrate has good water solubility and storage stability and is applicable to the treatment and prevention of human or animal infectious diseases caused by sensitive bacterium of gram-positive or-negative bacterium.

The invention discloses a diluent formula for improving the frozen quality of yak frozen semen. Each 100 milliliters of frozen semen diluent is prepared from 0.9 to 1.2 grams of sodium citrate, 3 to 5grams of saccharose, 6 to 8 milligrams of low density lipoprotein, 4 to 7 milliliters of glycerinum, 6 to 8 milligrams of resveratrol, 5 to 8 milligrams of melatonin, 35 milligrams of spectinomycin and 80 thousand units of gentamicin. According to the diluent formula disclosed by the invention, the low density lipoprotein, the resveratrol and the melatonin are jointly added into a semen diluent for the first time, and a diluent preparation method is corrected. Experimental data show that the frozen semen diluent not only can improve sperm motility and acrosomal intactness rate of frozen-thawed semen, but also improves activity of catalase (CAT), glutathione peroxidase (GR) and glutathione reductase (GSH) in seminal plasma which are used for protecting sperm fertilizing ability; furthermore, sperm DNA integrity is improved, and the fact that the semen formula can improve the frozen quality of semen is ensured.

The invention belongs to the technical field of biology, and discloses a method for obtaining a new variety of high-resistance white moth plasmid transgenic Bt poplar. The method comprises the following steps of cloning an artificially-modified Bt-cry1C gene into a new poplar plasmid transformation vector pYY20, performing enzyme digestion verification and PCR verification, and then performing sequence verification to obtain a plasmid transformation vector pYY25 of a Bt-cry1C gene; using gold powders to wrap pYY25 plasmid DNA and introducing the pYY25 plasmid DNA into a chloroplast genome of poplar through a gene gun transformation method, screening by spectinomycin to obtain a plurality of resistant buds, testing by Southern blot to obtain two positive resistant buds, carrying out three-round blade resistance regeneration to obtain homogenized transgenic Bt-cry1C poplar plants. The plasmid transgenic Bt-cry1C poplar is highly lethal to American white moth larvae, thereby obtaining thenew variety of high-resistance white moth plasmid transgenic Bt poplar.