86 results about "Macrolide resistance" patented technology

The invention discloses trichoderma asperellum TS7-1 capable of efficiently degrading macrolide antibiotics and application of the trichoderma asperellum TS7-1, and belongs to the technical field of microorganisms. The preservation number of the trichoderma asperellum TS7-1 is CGMCC (China General Microbiological Culture Collection Center) No. 41372. The Trichoderma asperellum TS7-1 (CGMCC No.41372) provided by the invention has high-efficiency degradation capability on various macrolide antibiotics (erythromycin, roxithromycin and azithromycin), the removal rates of the Trichoderma asperellum TS7-1, roxithromycin and azithromycin in 36h all reach 85% or above, and the Trichoderma asperellum TS7-1 has high-concentration antibiotic tolerance, degradation rate and degradation spectrum obviously superior to those of other strains in the prior art. The trichoderma asperellum TS7-1 is directly separated from a natural soil body which is not polluted by antibiotics, has no animal and plant pathogenicity hidden danger, has no microbial toxicity to degradation products of macrolide antibiotics, avoids secondary pollution, and has excellent environmental safety.

The present invention relates to the field of analytical chemistry, and discloses a mass spectrum ionization element for macrolide antibiotic detection and a preparation method thereof, the mass spectrum ionization element comprises: a conductive substrate made of stainless steel; the adsorption layer is attached to at least part of the surface of the substrate, the adsorption layer is formed by a boric acid functionalized covalent organic framework, and the boric acid functionalized covalent organic framework is of a polycyclic structure composed of repeated units shown in the formula I in the specification. The mass spectrum ionization element is uniform in coating, high in selectivity on macrolide enriched antibiotics in a complex matrix and high in enrichment capacity, an eluent of the mass spectrum ionization element can be used for detection based on a liquid chromatography method and can also serve as a mass spectrum ion source solid substrate, mass spectrum detection is conducted through electrospray ionization, the detection steps are simple, and the mass spectrum ionization element is suitable for mass spectrum detection. And the detected background noise is low.

According to the scheme, the invention provides the recombinant macrolide enzyme as well as the preparation method and the application thereof, the amino acid sequence of the recombinant macrolide enzyme is as shown in SEQ ID NO.1, and the recombinant macrolide enzyme is derived from an EreC esterase family of enterobacter hormaechei and is obtained through site-specific mutagenesis; the mutation sites are as follows: glutamic acid at the 44th site is mutated into asparagine, tyrosine at the 55th site is mutated into proline, proline at the 76th site is mutated into arginine, phenylalanine at the 153rd site is mutated into alanine, and serine at the 219th site is mutated into glutamic acid. The method is expected to remove macrolide antibiotic pollution, and has good economic benefits and practical values.

The present invention provides a method for enhancing the removal of resistance genes and promoting humification in aerobic composting. The method comprises the following steps: mixing livestock and poultry manure with a composting auxiliary material, adjusting the moisture content to 55%-65%, and adding a MnFe2O4 additive to obtain a composting raw material; aerobically composting the material, turning the compost every 2-3 days during a temperature rise period and a high temperature period, and every 7 days during a temperature fall period and a mature period, with the aerobic composting time being 20-30 days. Compared with a conventional composting method, the relative abundances of sulfonamide, tetracycline, macrolide, and aminoglycoside resistance genes in the mature compost obtained by the method are reduced by 60%-73%, 50%-57%, 20%-80%, and 45%-66%, respectively, and the humic acid (HA) content is increased by about 15%. Meanwhile, the method achieves efficient harmlessness and resource utilization of organic solid waste, and has the potential for large-scale production.

A method for enhancing resistance gene removal and promoting humification in an aerobic compost, which method comprises mixing livestock and poultry manure with compost auxiliary materials, adjusting the water content, adding an MnFe2O4 additive, and then performing aerobic composting for 20-30 days. Compared with conventional composting methods, the mature compost product obtained by means of the method exhibits a 60%-73% reduction in the relative abundance of sulfonamide resistance genes, a 50%-57% reduction in tetracycline resistance genes, a 20%-80% reduction in macrolide resistance genes, and a 45%-66% reduction in aminoglycoside resistance genes, and shows approximately a 15% increase in humic acid HA. This method achieves both efficient harmless treatment and resource utilization of organic solid waste.

The invention relates to a novel 16-membered macrolide compound 13alpha-O-alpha-L-oleandrosyl-23-alpha-hydroxy-25-isopropyl milbemycin beta3, a preparation method of the novel 16-membered macrolide compound 13alpha-O-alpha-L-oleandrosyl-23-alpha-hydroxy-25-isopropyl milbemycin beta3, and an application of the novel 16-membered macrolide compound 13alpha-O-alpha-L-oleandrosyl-23-alpha-hydroxy-25-isopropyl milbemycin

The invention discloses a method for preparing an ester compound and a macrolide compound, and belongs to the technical field of organic chemistry, the preparation method of the ester compound is as follows: in an organic solvent, an alpha-carbonyl alkenyl ester compound reacts with alcohol or phenol under the catalytic condition of alkali to obtain the ester compound; the preparation method of the macrolide compound comprises the following step: in an organic solvent, carrying out intramolecular hydroxyl reaction on an alpha-carbonyl alkenyl ester compound under the catalysis condition of alkali or acid, thereby obtaining the macrolide compound. The method is wide in substrate range, high in practicability and high in yield, racemization of a carboxylic acid alpha-chiral center can be inhibited in intermolecular esterification reaction and macrocyclic lactonization reaction, and the method can be applied to total synthesis of a Brevicidine natural product containing a 13-membered cyclic ester peptide core structure; the method disclosed by the invention has the advantages of mild reaction conditions, simplicity, easiness in operation, wide substrate adaptability, no racemization of the product and the like.

According to the scheme, the recombinant macrolide enzyme and the preparation method and application thereof are provided, the amino acid sequence of the recombinant macrolide enzyme is shown as SEQ ID NO.1, and the recombinant macrolide enzyme is obtained by site-directed mutagenesis of an erythromycin esterase family protein sequence derived from enterobacter hormaechei; the mutation sites are as follows: the 44 glutamic acid is mutated into asparagine, the 55 tyrosine is mutated into proline, the 153 proline is mutated into alanine, and the 219 serine is mutated into glutamic acid, so that the specific macrolide lactonase which is efficient, stable, easy to prepare on a large scale and more suitable for environmental requirements is prepared, and macrolide antibiotic pollution is removed; and the method has good economic benefits and practical values.

The invention discloses a novel Fe-C-N-coated Au material and a preparation method and application thereof.According to the Fe-C-N-coated Au material, iron monatomic nano-enzyme Fe-C-N serves as a core, gold nanoparticles are loaded on the surface, the Fe-C-N nanoparticles and a HAuCl4 aqueous solution are stirred at low temperature, then an ice-cold NaBH4 solution is slowly added into a mixed solution, and the Fe-C-N-coated Au material is obtained after reaction and treatment. The novel Fe-C-N-coated Au material disclosed by the invention can be used for rapidly and sensitively detecting the azithromycin or macrolide drug-resistant gene ermB.

Provided herein are pharmaceutically acceptable compositions containing macrolide antibiotics, in particular azithromycin. In particular, compositions containing azithromycin with low toxicity, especially for administration to felines, are provided herein.

The present invention provides a composition with a solubilizing effect, a preparation method thereof, and an application thereof. The composition comprises a solubilizing agent, a glidant, and an inclusion compound, wherein the mass ratios of the components are as follows: the mass ratio of the solubilizing agent to the inclusion compound is 1:1-1:20, and the mass ratio of the glidant to the inclusion compound is 1:100-3:20. The composition of the present invention can effectively improve the solubility of macrolides, tetracyclines, and penicillins, and the composition of the present invention does not react with the active ingredients of the drugs, ensuring that after the drugs are mixed therewith, the active ingredients of the drugs are stable, and the mixture of the drugs and the composition does not agglomerate or discolor.

The application discloses a preparation method and application of a cobalt monatomic doped strontium titanate composite material, and belongs to the field of water treatment. The cobalt doped strontium titanate composite catalytic material is composed of a cobalt metal doped strontium titanate material. A preparation process uses tetrabutyl titanate and a nitrate as reactants, ethylene glycol and water as solvents, and prepares strontium titanate (SrTiO3: STO) through a hydrothermal method. Then, cobalt monatomic atoms are doped into the strontium titanate through cobalt chloride (a cobalt source) by using an electrostatic adsorption method, so as to form cobalt doped strontium titanate (Co-STO). The material can efficiently activate an oxidant peracetic acid and rapidly degrade various antibiotic pollutants, and especially performs well in degrading quinolone, tetracycline and macrolide antibiotics. The catalytic material has the advantages of low cost, renewability and environmental protection, and is very suitable for being applied to a deep treatment process of pharmaceutical wastewater.

The invention relates to a solid phase extraction-ultra-high performance liquid chromatography-tandem mass spectrometry rapid determination method for antibiotics in aquaculture fresh water, which comprises the following steps: (1) adding an internal standard solution into aquaculture fresh water to be determined, uniformly mixing, filtering the obtained water sample, and adjusting the pH value; (2) activating a small solid-phase extraction column, enriching the water sample obtained in the step (1) through the small solid-phase extraction column, then leaching and eluting, collecting an eluent, blowing the eluent until the eluent is nearly dry, and fixing the volume by using methanol and water; and (3) feeding the solution obtained after volume metering in the step (2) into ultra-high performance liquid chromatography-tandem mass spectrometry for detection. The invention establishes a method for simultaneously detecting 37 antibiotic residues in aquaculture fresh water by solid phase extraction-ultra-high performance liquid chromatography-tandem mass spectrometry, the method comprises six common antibiotics such as tetracyclines, macrolides, fluoroquinolones, phenols, sulfonamides and cephalospores, the detection is convenient and fast, and the accuracy is high.

The present invention relates to a compound drug for preventing and treating porcine reproductive and respiratory syndrome (PRRS), belonging to the field of veterinary drugs. The present invention makes an oral pharmaceutical preparation by mixing an animal-specific macrolide drug, sodium houttuyfonate, mannan oligosaccharide peptide and pharmaceutical excipients in a certain proportion. The drug of the present invention can effectively treat acute-phase PRRS, rapidly relieve the clinical symptoms of diseased pigs and improve the survival rate.

The invention relates to the technical field of pesticide residue detection, in particular to a method for rapidly screening neonicotinoid and macrolide pesticide residues in vegetables through trichogramma chilonis. The 10% lethal effect condition of the trichogramma on nine kinds of common neonicotinoid or macrolide pesticides such as imidacloprid, acetamiprid, dinotefuran, thiamethoxam, clothianidin, nitenpyram, abamectin, methylamino abamectin benzoate and ivermectin is determined; acute toxic effects and action rules of nine common neonicotinoid or macrolide pesticides in vegetables on trichogramma are researched; according to the method, the 1-hour action dose of the pesticide is determined by adopting 10% of death effect, the detection limit of the trichogramma biotoxicity method on the residues of the 9 pesticides in the vegetables is calculated by combining the quality and surface area of the vegetables, and finally, the common neonicotinoid or macrolide pesticide residues in the vegetables are rapidly screened by adopting the trichogramma biotoxicity limit value determination method.

The invention relates to a method for analyzing pollutants in hospital wastewater based on liquid chromatography-high resolution mass spectrometry. The method comprises the following steps: S1, enriching pollutants in hospital wastewater through solid-phase extraction of four specific solid-phase extraction columns, and then analyzing and detecting a liquid to be detected through a specific chromatographic column, a specific liquid chromatography and a high-resolution mass spectrometry which are connected in series; the identification of various pollutants (medicines and metabolites thereof) in the hospital wastewater is realized, so that a data basis is provided for subsequent fingerprint analysis. Specifically, the analysis method provided by the invention can identify 34 kinds of substances, including sulfonamide antibiotics, macrolide antibiotics, cephalosporin antibiotics, quinolone antibiotics, beta-lactam antibiotics, aminoglycoside antibiotics and carbamazepine and metabolites thereof.

The application discloses a biosynthesis gene cluster of a polyene macrolide natural product mandimycin, and a natural product and application of the biosynthesis gene cluster, wherein the nucleotide sequence of the biosynthesis gene cluster is shown as SEQ ID NO. 1, and the compound structural formula of the natural product mandimycin and mandimycin B is shown as formula I and formula II. The natural product mandimycin can target fungal cell membrane phospholipid molecules, especially phosphatidylinositol, cause important ion efflux in fungal cells, and lead to fungal cell death. The natural product mandimycin and mandimycin B have strong in-vivo and in-vitro antibacterial activity and a broad antibacterial spectrum on various WHO-published multiple drug-resistant fungal key pathogens, including candida, aspergillus, cryptococcus, mucor and fusarium.

To provide a technique relating to total secretion regulation of sebaceous gland cells.SOLUTION: The present invention provides a total secretion regulator comprising a compound selected from the group consisting of oligosaccharides and macrolide compounds as an active ingredient.SELECTED DRAWING: None

We report improved adjuvant compounds that have an aryl 2-aminoimidazole structure for macrolide potentiation against a virulent strain of gram-negative bacteria, AB5075. Compounds were discovered to retain significant adjuvant activity at 10 μM, lowering the minimum inhibitory concentration (MIC) of clarithromycin (CLR) from 8-fold to 128-fold or greater. 2-Aminoimidazole compounds linked to aryl groups via either an amide or urea linker showed significantly improved activity over control compounds.

Provided herein are synthetic methods and intermediates useful in the preparation of polyketides, including 12-membered ring macrolides. These synthetic methods and intermediates provide scalable access to polyketides, including polyketides with potent bioactive splicing modulator activity and therapeutic potential, including treating various types of cancer. The polyketides include 12-membered ring macrolides, including Compound A, Compound B, and pladienolides, including pladienolide A, pladienolide B, pladienolide C, pladienolide D, pladienolide E, pladienolide F, and pladienolide G.

This invention discloses a method for controlling potato scab, relating to the field of crop cultivation methods. The method includes: Step S1, preparing a coating agent: mixing a first active ingredient and a second active ingredient, wherein the first active ingredient is selected from neonicotinoid insecticides or macrolide insecticides, and the second active ingredient is selected from at least one of methoxyacrylates, organosulfur compounds, or inorganic copper fungicides; Step S2, seed potato coating treatment: mixing the coating composition with potato seed potatoes to ensure the composition is uniformly adhered to the seed potato surface; Step S3, sowing. This invention, through the synergistic combination of insecticides and fungicides, controls pathogens and pests at the source, combining growth-stage control and soil treatment to construct a comprehensive, multi-effect control method, improving control efficacy, reducing pesticide usage, and achieving highly efficient control of potato scab.

The present invention discloses a class of 24-membered macrolide compounds, their preparation methods, and applications. Three macrolide compounds [1-3] with glycosyl side chains were prepared from Bacillus amyloliquefaciens SCSIO 41392. As shown in formula (I), compounds 1-3 are newly reported compounds that can significantly inhibit the production of virulence factors of Pseudomonas aeruginosa. Compounds 1 and 2 can inhibit the PQS quorum sensing system of Pseudomonas aeruginosa and the production of the virulence factor "pyocyanin" regulated by it, while compound 3 can significantly inhibit the production of the virulence factor "siderophore" of Pseudomonas aeruginosa. Therefore, compounds 1-3 can be used to develop antibacterial drugs. The present invention provides new applications for the polar bacterium Bacillus amyloliquefaciens SCSIO 41392 and provides alternative lead compounds for the development of new antibacterial drugs derived from polar microorganisms.

A new class of dimeric 2-aminoimidazole (2-AI) compounds that potentiate macrolide antibiotics against A. baumannii. A parent dimer lowers the MIC of clarithromycin (CLR) from 32 μg / mL to 1 μg / mL at a concentration of 7.5 μM (3.4 μg / mL), while a structure activity relationship (SAR) study on the dimeric 2-AI scaffold resulted in the identification of several compounds with increased activity. Substitution of fluorine on a central phenyl ring resulted in the most potent activity, with the lead compound, containing a fluorine ortho to each of the 2-AIs, that lowered the CLR MIC to 2 μg / mL against AB5075 at 1.5 μM (0.72 μg / mL), exceeding the activity of both the parent dimer and the lead aryl-2-AI. Furthermore, these dimeric 2-AI analogs exhibit favorably low mammalian cell toxicity.

The invention relates to the field of medicines, in particular to a hydroxypyridone coupled macrolide compound shown in a formula (I) or a formula (II) and application of the hydroxypyridone coupled macrolide compound in preparation of an antibacterial agent and a bacterial biofilm inhibitor. In-vivo and in-vitro pharmacological verification shows that most of the hydroxypyridone coupled macrolide compounds have antibacterial activity, can be used for preparing antibacterial agents and anti-biofilm agents, and have the potential of being developed into novel antibacterial drugs.

This invention relates to the field of agricultural biological control technology, specifically disclosing a novel bafilomycin analogue with antifungal activity, its preparation method, and its application. The bafilomycin analogue is a novel natural compound obtained by fermentation culture of Streptomyces sp. TH21, component activity tracing, and subsequent separation and extraction. This type of compound exhibits good activity against plant pathogenic fungi and has the potential to be developed into a novel, highly efficient, and environmentally friendly biological fungicide.

The application discloses a synergistic bactericidal composition, comprising the following components: a toona sinensis extract: 16-2048 mu g / mL; a macrolide antibiotic: 0.0625-4096 mu g / mL; the bacteria are staphylococcus aureus or methicillin-resistant staphylococcus aureus, and the macrolide antibiotic is erythromycin or azithromycin. The application further discloses a use of the synergistic bactericidal composition in preparation of a medicine for treating skin and soft tissue infections. The composition provided by the application can convert the traditional macrolide antibiotics such as erythromycin and azithromycin which only have a bacteriostatic effect into a preparation which has clear bactericidal activity on staphylococcus aureus and drug-resistant strains thereof. After the toona sinensis extract is combined with the antibiotic, a synergistic effect is exhibited, and the concentration of the toona sinensis extract and the antibiotic required when the toona sinensis extract and the antibiotic are used alone can be greatly reduced.

The invention relates to a molecular marker of drug-resistant mycobacterium abscessus based on fusA gene mutation and application of the molecular marker, and belongs to the technical field of biological medicines. The invention provides a group of molecular markers for detecting the drug resistance of mycobacterium abscessus. The molecular markers are mutations on a fusA gene of the mycobacterium abscessus, including fusA1318Agt; g, fusA 1613 G gt; a, fusA18721883 dup GGGCGACGTGAT, and the number of the GGGCGACGTGAT is 1: 1. The invention finds that when the mycobacterium abscessus generates fusA1613 G gt; when A is mutated, cross resistance is generated to aminoglycoside drugs, macrolide drugs and tetracycline drug tigecycline. The fusA < 1318 > A < gt > is generated; the mycobacterium abscessus with mutations of G and fusA18721883 dup GGGCGACGTGAT is resistant to aminoglycoside drugs and tigecycline, and meanwhile, the mycobacterium abscessus has lateral sensitivity to macrolide drugs. The mycobacterium abscessus with mutations of G and fusA18721883 dup GGGCGACGTGAT is resistant to aminoglycoside drugs and tigecycline. The method is of great significance to clinical molecular diagnosis and medication.

This invention discloses a screening and detection method for antibiotic residues in organic fertilizers, relating to the field of trace residue detection. The detection method includes the following steps: S1, mixing the organic fertilizer sample with a buffer solution to obtain an extract; S2, adding a divalent calcium ion compound to the extract, mixing well, allowing it to stand, centrifuging, discarding the supernatant, and collecting the precipitate; S3, adding an alkaline releasing agent to the precipitate to dissolve it, centrifuging, collecting the supernatant, and obtaining a release solution; S4, reacting the release solution with a colorimetric reagent, measuring the visible light absorbance, and calculating the antibiotic content based on a standard curve. This method not only completely avoids the destruction of antibiotics by strong acids but also transforms humic acid from an interfering substance into an enrichment tool, thereby significantly improving the extraction rate. It is simple to operate and low in cost, thus providing a novel solution for the detection of macrolide antibiotic residues in organic fertilizers.

The present invention relates to a macrolide compound, and a preparation method therefor and the use thereof. Specifically disclosed is a compound as shown in formula I or a pharmaceutically acceptable salt thereof. The compound of the present invention exhibits a significant antibacterial activity, comprising the antibacterial activity against bacteria resistant to macrolide drugs such as azithromycin. The compound described herein has a low hepatotoxicity and superior safety compared to other macrolide compounds. The compound can be used for infectious diseases, etc.