105 results about "Macrolide resistance" patented technology

The invention discloses trichoderma asperellum TS7-1 capable of efficiently degrading macrolide antibiotics and application of the trichoderma asperellum TS7-1, and belongs to the technical field of microorganisms. The preservation number of the trichoderma asperellum TS7-1 is CGMCC (China General Microbiological Culture Collection Center) No. 41372. The Trichoderma asperellum TS7-1 (CGMCC No.41372) provided by the invention has high-efficiency degradation capability on various macrolide antibiotics (erythromycin, roxithromycin and azithromycin), the removal rates of the Trichoderma asperellum TS7-1, roxithromycin and azithromycin in 36h all reach 85% or above, and the Trichoderma asperellum TS7-1 has high-concentration antibiotic tolerance, degradation rate and degradation spectrum obviously superior to those of other strains in the prior art. The trichoderma asperellum TS7-1 is directly separated from a natural soil body which is not polluted by antibiotics, has no animal and plant pathogenicity hidden danger, has no microbial toxicity to degradation products of macrolide antibiotics, avoids secondary pollution, and has excellent environmental safety.

The present invention relates to the field of analytical chemistry, and discloses a mass spectrum ionization element for macrolide antibiotic detection and a preparation method thereof, the mass spectrum ionization element comprises: a conductive substrate made of stainless steel; the adsorption layer is attached to at least part of the surface of the substrate, the adsorption layer is formed by a boric acid functionalized covalent organic framework, and the boric acid functionalized covalent organic framework is of a polycyclic structure composed of repeated units shown in the formula I in the specification. The mass spectrum ionization element is uniform in coating, high in selectivity on macrolide enriched antibiotics in a complex matrix and high in enrichment capacity, an eluent of the mass spectrum ionization element can be used for detection based on a liquid chromatography method and can also serve as a mass spectrum ion source solid substrate, mass spectrum detection is conducted through electrospray ionization, the detection steps are simple, and the mass spectrum ionization element is suitable for mass spectrum detection. And the detected background noise is low.

According to the scheme, the invention provides the recombinant macrolide enzyme as well as the preparation method and the application thereof, the amino acid sequence of the recombinant macrolide enzyme is as shown in SEQ ID NO.1, and the recombinant macrolide enzyme is derived from an EreC esterase family of enterobacter hormaechei and is obtained through site-specific mutagenesis; the mutation sites are as follows: glutamic acid at the 44th site is mutated into asparagine, tyrosine at the 55th site is mutated into proline, proline at the 76th site is mutated into arginine, phenylalanine at the 153rd site is mutated into alanine, and serine at the 219th site is mutated into glutamic acid. The method is expected to remove macrolide antibiotic pollution, and has good economic benefits and practical values.

The present invention provides a method for enhancing the removal of resistance genes and promoting humification in aerobic composting. The method comprises the following steps: mixing livestock and poultry manure with a composting auxiliary material, adjusting the moisture content to 55%-65%, and adding a MnFe2O4 additive to obtain a composting raw material; aerobically composting the material, turning the compost every 2-3 days during a temperature rise period and a high temperature period, and every 7 days during a temperature fall period and a mature period, with the aerobic composting time being 20-30 days. Compared with a conventional composting method, the relative abundances of sulfonamide, tetracycline, macrolide, and aminoglycoside resistance genes in the mature compost obtained by the method are reduced by 60%-73%, 50%-57%, 20%-80%, and 45%-66%, respectively, and the humic acid (HA) content is increased by about 15%. Meanwhile, the method achieves efficient harmlessness and resource utilization of organic solid waste, and has the potential for large-scale production.

A method for enhancing resistance gene removal and promoting humification in an aerobic compost, which method comprises mixing livestock and poultry manure with compost auxiliary materials, adjusting the water content, adding an MnFe2O4 additive, and then performing aerobic composting for 20-30 days. Compared with conventional composting methods, the mature compost product obtained by means of the method exhibits a 60%-73% reduction in the relative abundance of sulfonamide resistance genes, a 50%-57% reduction in tetracycline resistance genes, a 20%-80% reduction in macrolide resistance genes, and a 45%-66% reduction in aminoglycoside resistance genes, and shows approximately a 15% increase in humic acid HA. This method achieves both efficient harmless treatment and resource utilization of organic solid waste.

The invention relates to the field of new biological materials, in particular to a high-packaging-dose antibacterial slow-release biological patch obtained by packaging at least two antibiotics with an acellular matrix material. The acellular matrix material is derived from any two of small intestinal submucosa, peritoneum, dermis, bladder basement membrane and pericardium; the antibiotics comprise hydrophobic antibiotics and hydrophilic antibiotics, and the hydrophobic antibiotics are selected from one or more of Beta-lactam antibiotics, rifamycin and macrolide antibiotics; the hydrophilic antibiotics are tetracycline antibiotics. The coating-free drug-loaded sustained-release system based on a lamination process kills bacteria in a short period, prevents colonization and reproduction of bacteria in a wound / patch, realizes synergistic and continuous release of antibiotics along with a wound healing process, guides tissue regeneration and synchronously realizes degradation, is applied to a high-incidence infection period of a surgical operation, reduces adhesion, and has a good application prospect. The abdominal wall strength is enhanced, and the growth of wound granulation tissues is promoted.

The invention discloses a method for degrading residual antibiotics in macrolide antibiotic fermentation waste. The method comprises the following steps: taking antibiotic mushroom dregs as a degradation raw material, stainless steel beads as a grinding medium, and taking an oxidizing agent and a pro-oxidizing agent as ball milling aids. The mixture is subjected to a grinding reaction in a ball milling tank, treatment of the mushroom dregs is completed, the content of antibiotics in the mushroom dregs obtained after ball milling is reduced to 99% or below, the antibiotic resistance of the antibiotics disappears, and the mushroom dregs can be further used for re-fermentation of erythromycin. The method has the advantages that the process route is short, the reagent dosage is small, the price is low, operation is easy, the whole process is carried out under the solid-phase condition, no solvent is needed, the degradation efficiency is good, and the method has good application and popularization prospects.

The invention relates to a novel 16-membered macrolide compound 13alpha-O-alpha-L-oleandrosyl-23-alpha-hydroxy-25-isopropyl milbemycin beta3, a preparation method of the novel 16-membered macrolide compound 13alpha-O-alpha-L-oleandrosyl-23-alpha-hydroxy-25-isopropyl milbemycin beta3, and an application of the novel 16-membered macrolide compound 13alpha-O-alpha-L-oleandrosyl-23-alpha-hydroxy-25-isopropyl milbemycin

The invention discloses a method for preparing an ester compound and a macrolide compound, and belongs to the technical field of organic chemistry, the preparation method of the ester compound is as follows: in an organic solvent, an alpha-carbonyl alkenyl ester compound reacts with alcohol or phenol under the catalytic condition of alkali to obtain the ester compound; the preparation method of the macrolide compound comprises the following step: in an organic solvent, carrying out intramolecular hydroxyl reaction on an alpha-carbonyl alkenyl ester compound under the catalysis condition of alkali or acid, thereby obtaining the macrolide compound. The method is wide in substrate range, high in practicability and high in yield, racemization of a carboxylic acid alpha-chiral center can be inhibited in intermolecular esterification reaction and macrocyclic lactonization reaction, and the method can be applied to total synthesis of a Brevicidine natural product containing a 13-membered cyclic ester peptide core structure; the method disclosed by the invention has the advantages of mild reaction conditions, simplicity, easiness in operation, wide substrate adaptability, no racemization of the product and the like.

According to the scheme, the recombinant macrolide enzyme and the preparation method and application thereof are provided, the amino acid sequence of the recombinant macrolide enzyme is shown as SEQ ID NO.1, and the recombinant macrolide enzyme is obtained by site-directed mutagenesis of an erythromycin esterase family protein sequence derived from enterobacter hormaechei; the mutation sites are as follows: the 44 glutamic acid is mutated into asparagine, the 55 tyrosine is mutated into proline, the 153 proline is mutated into alanine, and the 219 serine is mutated into glutamic acid, so that the specific macrolide lactonase which is efficient, stable, easy to prepare on a large scale and more suitable for environmental requirements is prepared, and macrolide antibiotic pollution is removed; and the method has good economic benefits and practical values.

The invention discloses a novel Fe-C-N-coated Au material and a preparation method and application thereof.According to the Fe-C-N-coated Au material, iron monatomic nano-enzyme Fe-C-N serves as a core, gold nanoparticles are loaded on the surface, the Fe-C-N nanoparticles and a HAuCl4 aqueous solution are stirred at low temperature, then an ice-cold NaBH4 solution is slowly added into a mixed solution, and the Fe-C-N-coated Au material is obtained after reaction and treatment. The novel Fe-C-N-coated Au material disclosed by the invention can be used for rapidly and sensitively detecting the azithromycin or macrolide drug-resistant gene ermB.

The invention relates to the technical field of adsorption material preparation, in particular to Ti3C2Tx hollow microspheres and a preparation method and application thereof.The preparation method of the Ti3C2Tx hollow microspheres comprises the steps that S1, LiF is added into an HCl solution, first stirring is conducted till LiF is dissolved, Ti3AlC2 is added, etching, first centrifugation, washing, ultrasonic treatment and second centrifugation are conducted, and single-layer Ti3C2Tx dispersion liquid is obtained; s2, the single-layer Ti < 3 > C < 2 > T < x > dispersion liquid obtained in the step S1 and PS pellets are subjected to second stirring, filtering, drying and calcination, and Ti < 3 > C < 2 > T < x > hollow microspheres are obtained; wherein in S2, the mass ratio of the Ti < 3 > C < 2 > T < x > to the PS pellets is 1: (3-7); wherein in the step S2, the second stirring condition is as follows: the second stirring temperature is 20-30 DEG C, and the second stirring time is 12-48 hours. The Ti3C2Tx hollow microspheres provided by the invention have relatively fast adsorption kinetics and relatively large adsorption capacity for removing tetracycline and macrolide antibiotics, and are high in physical and chemical stability and strong in pollution resistance.

Provided herein are pharmaceutically acceptable compositions containing macrolide antibiotics, in particular azithromycin. In particular, compositions containing azithromycin with low toxicity, especially for administration to felines, are provided herein.

The present invention relates to the field of pharmaceutical technology, and specifically to the application of a macrolide derivative in the preparation of a drug for treating heart failure. The present invention provides a new use of a macrolide derivative. Experimental results show that this macrolide derivative significantly improves the cardiac function of heart failure mice, increases the ejection fraction (EF%), fractional shortening (FS%), and cardiac output per minute (CO), without changing the heart rate (HR), is equivalent to the positive drug digoxin, and is superior to enalapril; at the same time, it has the effects of reducing the heart weight ratio, improving myocardial hypertrophy and fibrosis, and has good clinical prospects and social benefits.

Disclosed herein are macrolide compounds having senescent cell elimination activity. These compounds selectively target senescent cells and can be used to reduce or eradicate senescent cells in a subject's body and / or delay the onset of aging. These compounds also inhibit the proliferation of cancer stem cells and can be used to treat cancer, particularly to inhibit the proliferation of cancer stem cells that cause metastasis and tumor recurrence.

The invention discloses a method for detecting macrolide antibiotics in a water body, which adopts a dispersive solid-phase extraction method and comprises the following steps: S1, synthesizing a material to obtain a metal organic framework material Ce-MOF of cerium; s2, sample collection: collecting river and lake water samples and transporting the river and lake water samples to a laboratory for analysis; s3, sample pretreatment; s4, analyzing the sample, detecting the macrolide antibiotics through a liquid chromatography tandem mass spectrometer, and adopting an ion electrospray positive ion mode ESI < + > and a multi-reaction monitoring MRM mode. Compared with traditional solid-phase extraction, the method has the advantages that the operation process is simplified, the complexity and time cost of sample treatment are reduced, the accuracy and repeatability are improved, the method is more environment-friendly, and the experimental detection cost is reduced.

The present invention provides a composition with a solubilizing effect, a preparation method thereof, and an application thereof. The composition comprises a solubilizing agent, a glidant, and an inclusion compound, wherein the mass ratios of the components are as follows: the mass ratio of the solubilizing agent to the inclusion compound is 1:1-1:20, and the mass ratio of the glidant to the inclusion compound is 1:100-3:20. The composition of the present invention can effectively improve the solubility of macrolides, tetracyclines, and penicillins, and the composition of the present invention does not react with the active ingredients of the drugs, ensuring that after the drugs are mixed therewith, the active ingredients of the drugs are stable, and the mixture of the drugs and the composition does not agglomerate or discolor.

The application discloses a preparation method and application of a cobalt monatomic doped strontium titanate composite material, and belongs to the field of water treatment. The cobalt doped strontium titanate composite catalytic material is composed of a cobalt metal doped strontium titanate material. A preparation process uses tetrabutyl titanate and a nitrate as reactants, ethylene glycol and water as solvents, and prepares strontium titanate (SrTiO3: STO) through a hydrothermal method. Then, cobalt monatomic atoms are doped into the strontium titanate through cobalt chloride (a cobalt source) by using an electrostatic adsorption method, so as to form cobalt doped strontium titanate (Co-STO). The material can efficiently activate an oxidant peracetic acid and rapidly degrade various antibiotic pollutants, and especially performs well in degrading quinolone, tetracycline and macrolide antibiotics. The catalytic material has the advantages of low cost, renewability and environmental protection, and is very suitable for being applied to a deep treatment process of pharmaceutical wastewater.

The invention discloses a preparation method and application of a cobalt monatomic doped strontium titanate composite material, and belongs to the field of water treatment. The cobalt-doped strontium titanate composite catalytic material disclosed by the invention is formed by compounding a cobalt metal-doped strontium titanate material. According to the preparation process, tetrabutyl titanate and nitrate are used as reactants, ethylene glycol and water are used as solvents, and strontium titanate (SrTiO3: STO) is prepared through a hydrothermal method. Then, doping cobalt single atoms into strontium titanate through cobalt chloride (a cobalt source) by using an electrostatic adsorption method to form cobalt-doped strontium titanate (Co-STO); the material can efficiently activate an oxidizing agent peracetic acid and rapidly degrade various antibiotic pollutants, and especially has excellent performance in the aspect of degrading quinolones, tetracyclines and macrolide antibiotics. The catalytic material has the advantages of low cost, reproducibility, environmental protection and the like, and is very suitable for the advanced treatment process of pharmaceutical wastewater.

The invention relates to a solid phase extraction-ultra-high performance liquid chromatography-tandem mass spectrometry rapid determination method for antibiotics in aquaculture fresh water, which comprises the following steps: (1) adding an internal standard solution into aquaculture fresh water to be determined, uniformly mixing, filtering the obtained water sample, and adjusting the pH value; (2) activating a small solid-phase extraction column, enriching the water sample obtained in the step (1) through the small solid-phase extraction column, then leaching and eluting, collecting an eluent, blowing the eluent until the eluent is nearly dry, and fixing the volume by using methanol and water; and (3) feeding the solution obtained after volume metering in the step (2) into ultra-high performance liquid chromatography-tandem mass spectrometry for detection. The invention establishes a method for simultaneously detecting 37 antibiotic residues in aquaculture fresh water by solid phase extraction-ultra-high performance liquid chromatography-tandem mass spectrometry, the method comprises six common antibiotics such as tetracyclines, macrolides, fluoroquinolones, phenols, sulfonamides and cephalospores, the detection is convenient and fast, and the accuracy is high.

The present invention relates to a compound drug for preventing and treating porcine reproductive and respiratory syndrome (PRRS), belonging to the field of veterinary drugs. The present invention makes an oral pharmaceutical preparation by mixing an animal-specific macrolide drug, sodium houttuyfonate, mannan oligosaccharide peptide and pharmaceutical excipients in a certain proportion. The drug of the present invention can effectively treat acute-phase PRRS, rapidly relieve the clinical symptoms of diseased pigs and improve the survival rate.

The present invention relates to a synthesis method of 20-aldehyde-23-piperidinyl-5-O-mycamine glycosyl-tylonolide, its salt and use. The present invention has in vitro antibacterial activity against pathogenic bacteria of livestock and poultry such as Pasteurella multocida, Streptococcus suis, Streptococcus pneumoniae, Staphylococcus aureus, Bordetella bronchiseptica, Klebsiella pneumoniae, Mannheimia hemolytica, Haemophilus parasuis, Actinobacillus pleuropneumoniae, etc., and its antibacterial activity is significantly superior to that of control drugs. The present invention is also superior to macrolide control drugs in antibacterial effect on mycoplasma such as chicken, gallinulina, and pig. It can be used to prepare new high-activity veterinary antibacterial and anti-mycoplasma drugs, and has broad application prospects in livestock and poultry and aquaculture.

The invention relates to the technical field of pesticide residue detection, in particular to a method for rapidly screening neonicotinoid and macrolide pesticide residues in vegetables through trichogramma chilonis. The 10% lethal effect condition of the trichogramma on nine kinds of common neonicotinoid or macrolide pesticides such as imidacloprid, acetamiprid, dinotefuran, thiamethoxam, clothianidin, nitenpyram, abamectin, methylamino abamectin benzoate and ivermectin is determined; acute toxic effects and action rules of nine common neonicotinoid or macrolide pesticides in vegetables on trichogramma are researched; according to the method, the 1-hour action dose of the pesticide is determined by adopting 10% of death effect, the detection limit of the trichogramma biotoxicity method on the residues of the 9 pesticides in the vegetables is calculated by combining the quality and surface area of the vegetables, and finally, the common neonicotinoid or macrolide pesticide residues in the vegetables are rapidly screened by adopting the trichogramma biotoxicity limit value determination method.

The invention relates to a method for analyzing pollutants in hospital wastewater based on liquid chromatography-high resolution mass spectrometry. The method comprises the following steps: S1, enriching pollutants in hospital wastewater through solid-phase extraction of four specific solid-phase extraction columns, and then analyzing and detecting a liquid to be detected through a specific chromatographic column, a specific liquid chromatography and a high-resolution mass spectrometry which are connected in series; the identification of various pollutants (medicines and metabolites thereof) in the hospital wastewater is realized, so that a data basis is provided for subsequent fingerprint analysis. Specifically, the analysis method provided by the invention can identify 34 kinds of substances, including sulfonamide antibiotics, macrolide antibiotics, cephalosporin antibiotics, quinolone antibiotics, beta-lactam antibiotics, aminoglycoside antibiotics and carbamazepine and metabolites thereof.

The application discloses a biosynthesis gene cluster of a polyene macrolide natural product mandimycin, and a natural product and application of the biosynthesis gene cluster, wherein the nucleotide sequence of the biosynthesis gene cluster is shown as SEQ ID NO. 1, and the compound structural formula of the natural product mandimycin and mandimycin B is shown as formula I and formula II. The natural product mandimycin can target fungal cell membrane phospholipid molecules, especially phosphatidylinositol, cause important ion efflux in fungal cells, and lead to fungal cell death. The natural product mandimycin and mandimycin B have strong in-vivo and in-vitro antibacterial activity and a broad antibacterial spectrum on various WHO-published multiple drug-resistant fungal key pathogens, including candida, aspergillus, cryptococcus, mucor and fusarium.

To provide a technique relating to total secretion regulation of sebaceous gland cells.SOLUTION: The present invention provides a total secretion regulator comprising a compound selected from the group consisting of oligosaccharides and macrolide compounds as an active ingredient.SELECTED DRAWING: None

We report improved adjuvant compounds that have an aryl 2-aminoimidazole structure for macrolide potentiation against a virulent strain of gram-negative bacteria, AB5075. Compounds were discovered to retain significant adjuvant activity at 10 μM, lowering the minimum inhibitory concentration (MIC) of clarithromycin (CLR) from 8-fold to 128-fold or greater. 2-Aminoimidazole compounds linked to aryl groups via either an amide or urea linker showed significantly improved activity over control compounds.

Provided herein are synthetic methods and intermediates useful in the preparation of polyketides, including 12-membered ring macrolides. These synthetic methods and intermediates provide scalable access to polyketides, including polyketides with potent bioactive splicing modulator activity and therapeutic potential, including treating various types of cancer. The polyketides include 12-membered ring macrolides, including Compound A, Compound B, and pladienolides, including pladienolide A, pladienolide B, pladienolide C, pladienolide D, pladienolide E, pladienolide F, and pladienolide G.

This invention discloses a method for controlling potato scab, relating to the field of crop cultivation methods. The method includes: Step S1, preparing a coating agent: mixing a first active ingredient and a second active ingredient, wherein the first active ingredient is selected from neonicotinoid insecticides or macrolide insecticides, and the second active ingredient is selected from at least one of methoxyacrylates, organosulfur compounds, or inorganic copper fungicides; Step S2, seed potato coating treatment: mixing the coating composition with potato seed potatoes to ensure the composition is uniformly adhered to the seed potato surface; Step S3, sowing. This invention, through the synergistic combination of insecticides and fungicides, controls pathogens and pests at the source, combining growth-stage control and soil treatment to construct a comprehensive, multi-effect control method, improving control efficacy, reducing pesticide usage, and achieving highly efficient control of potato scab.