5 results about "Lactonase" patented technology

According to the scheme, the recombinant macrolide enzyme and the preparation method and application thereof are provided, the amino acid sequence of the recombinant macrolide enzyme is shown as SEQ ID NO.1, and the recombinant macrolide enzyme is obtained by site-directed mutagenesis of an erythromycin esterase family protein sequence derived from enterobacter hormaechei; the mutation sites are as follows: the 44 glutamic acid is mutated into asparagine, the 55 tyrosine is mutated into proline, the 153 proline is mutated into alanine, and the 219 serine is mutated into glutamic acid, so that the specific macrolide lactonase which is efficient, stable, easy to prepare on a large scale and more suitable for environmental requirements is prepared, and macrolide antibiotic pollution is removed; and the method has good economic benefits and practical values.

The application provides a hot liquid source quorum quenching strain Shiquan Haeyuan bacteria and application thereof, the strain screened can inhibit pathogenic bacteria and is used for preparing bacteriostatic drugs; the preservation number of the Shiquan Haeyuan bacteria screened is CCTCC NO: M 20251224.The application also provides an AHL degradation enzyme Aii937 protein separated from the YESM3 strain, characterized in that the amino acid sequence of the protein is SEQ ID NO: 2.The YESM3 strain of the application can block the QS path of pathogenic bacteria through AHL lactonase Aii937, reduce the secretion of virulence factors of pathogenic bacteria; the strain YESM3 has no pathogenicity.AHL lactonase does not have broad spectrum, has strong substrate specificity, and only degrades C6-HSL and 3OC-C 14 -HSL.

This invention provides a method derived from Bacillus ( Bacillus The lactone enzyme AiiA (sp.) GB-1 and its application are described, with its amino acid sequence shown in SEQ ID NO:1. Experimental results show that the enzyme has degradation activity against various N-acyl homoserine lactone signaling molecules, exhibits high degradation ability against long-chain AHLs, and has good stability within a certain temperature and pH range. Further experimental results show that the AiiA enzyme can reduce the quorum sensing-related phenotype of pathogens. Simultaneously, exogenous addition of AiiA enzyme can also reduce the degree of pathogen infection in plant tissues, indicating that the enzyme can be used for plant disease control through engineered bacteria expression or exogenous enzyme preparations. This invention provides a new technical approach for green control of plant diseases.

The present invention relates to oral care compositions comprising a peptidase or lactonase, preferably a peptidase and a lactonase, the use of said compositions in medicine, the use of said compositions in the treatment of oral diseases, methods of treatment comprising the administration of said compositions to a human subject, methods of preventing or removing oral biofilm comprising contacting an oral biofilm with said compositions, methods for reducing the risk of oral biofilm formation, and kits comprising said compositions.

This invention discloses a method derived from Bacillus ( Bacillus The population quenching lactonease YtnP gene of sp.) GB-1, the nucleotide sequence of which is shown in SEQ ID NO:1, was introduced into the soft rot pathogen via a two-parent hybridization method. Heterologous overexpression of this gene significantly inhibited biofilm formation, motility, and host pathogenicity of the soft rot pathogen. It was also transferred into Arabidopsis thaliana via Agrobacterium-mediated transformation and overexpressed in Arabidopsis. ytnP Genes can significantly enhance plant resistance to soft rot, and this invention provides new resources and technical approaches for the prevention and control of soft rot.