26 results about "Lactonase" patented technology

According to the scheme, the invention provides the recombinant macrolide enzyme as well as the preparation method and the application thereof, the amino acid sequence of the recombinant macrolide enzyme is as shown in SEQ ID NO.1, and the recombinant macrolide enzyme is derived from an EreC esterase family of enterobacter hormaechei and is obtained through site-specific mutagenesis; the mutation sites are as follows: glutamic acid at the 44th site is mutated into asparagine, tyrosine at the 55th site is mutated into proline, proline at the 76th site is mutated into arginine, phenylalanine at the 153rd site is mutated into alanine, and serine at the 219th site is mutated into glutamic acid. The method is expected to remove macrolide antibiotic pollution, and has good economic benefits and practical values.

The present invention relates to an oral care composition comprising a peptidase or lactonase, preferably a peptidase and a lactonase, use of said composition in medicine, use of said composition in treatment of oral disease, methods of treatment comprising administering said composition to a human subject, methods of preventing or removing oral biofilm comprising contacting an oral biofilm with said composition, methods for reducing the risk of oral biofilm formation, and kits of parts comprising said composition.

The invention discloses a rare-earth-based bionic nano material as well as a preparation method and application thereof, and relates to the field of microbial pollution and anti-corrosion treatment. According to the invention, the active center of lactonase can be simulated by using a zinc-based material, and ester bonds in N-acyl homoserine lactone (AHL) molecules can be hydrolyzed by using the lactonase so as to block bacterial quorum sensing (QS) among bacterial florae, so that the antibacterial effect is achieved. Meanwhile, the outer layer of zinc is provided with a cerium element as a shell, so that on one hand, permeation of oxygen, moisture and corrosive ions can be prevented; on the other hand, the structure has hydrophobicity, and corrosion of moisture and salt ions to a metal matrix is further reduced through the hydrophobicity. Cerium and an oxide thereof have certain antibacterial ability and can have a synergistic effect with zinc, Ce can promote generation of Zn-N and generate strong electron interaction, better hydrophobicity is shown, and attachment and growth of microorganisms on the metal surface can be further inhibited.

The present invention relates to an oral care composition comprising a peptidase or lactonase, preferably a peptidase and a lactonase, use of said composition in medicine, use of said composition in treatment of oral disease, methods of treatment comprising administering said composition to a human subject, methods of preventing or removing oral biofilm comprising contacting an oral biofilm with said composition, methods for reducing the risk of oral biofilm formation, and kits of parts comprising said composition.

The invention discloses a production method for improving the ring opening rate of monascus milovastatin, and belongs to the technical field of monascus rice production. According to the method, a monascus purpureus high-yield strain is selected and inoculated into a solid indica rice matrix containing a specific additive for fermentation after spore activation, and the matrix comprises 1, 5-p-coumaroylquinic acid, sodium acetate, sodium citrate, a thermally modified soybean protein isolate polysaccharide sulfate compound and other components. Wherein sodium acetate and sodium citrate can serve as short-chain fatty acid precursors, the activity of closed-loop lactonase is inhibited through pH regulation, and generation of lactone type products is reduced; 1, 5-p-coumaroylquinic acid activates the expression of a polyketide synthase gene cluster, so that the synthesis flux of the Monacolin K with an open-loop structure is improved; nicotinamide ribose enhances the electron transfer efficiency of glycolysis and TCA circulation, provides sufficient ATP for polyketone synthesis, and promotes synthesis of Monacolin K. The compound further strengthens the synthesis flux of secondary metabolites by changing the form of hyphae and continuously releasing a nitrogen / carbon source, and promotes intracellular products to be transferred to extracellular.

According to the scheme, the recombinant macrolide enzyme and the preparation method and application thereof are provided, the amino acid sequence of the recombinant macrolide enzyme is shown as SEQ ID NO.1, and the recombinant macrolide enzyme is obtained by site-directed mutagenesis of an erythromycin esterase family protein sequence derived from enterobacter hormaechei; the mutation sites are as follows: the 44 glutamic acid is mutated into asparagine, the 55 tyrosine is mutated into proline, the 153 proline is mutated into alanine, and the 219 serine is mutated into glutamic acid, so that the specific macrolide lactonase which is efficient, stable, easy to prepare on a large scale and more suitable for environmental requirements is prepared, and macrolide antibiotic pollution is removed; and the method has good economic benefits and practical values.

The application provides a hot liquid source quorum quenching strain Shiquan Haeyuan bacteria and application thereof, the strain screened can inhibit pathogenic bacteria and is used for preparing bacteriostatic drugs; the preservation number of the Shiquan Haeyuan bacteria screened is CCTCC NO: M 20251224.The application also provides an AHL degradation enzyme Aii937 protein separated from the YESM3 strain, characterized in that the amino acid sequence of the protein is SEQ ID NO: 2.The YESM3 strain of the application can block the QS path of pathogenic bacteria through AHL lactonase Aii937, reduce the secretion of virulence factors of pathogenic bacteria; the strain YESM3 has no pathogenicity.AHL lactonase does not have broad spectrum, has strong substrate specificity, and only degrades C6-HSL and 3OC-C 14 -HSL.

The present invention relates to the field of biology, and specifically to the application of PpL in inhibiting the formation of Gram-negative bacterial biofilms, inhibitors, and methods for preparing hexameric modifications. The PpL of the present invention has higher thermal stability and exhibits a biofilm inhibitory effect on Gram-negative bacteria, especially Acinetobacter baumannii. PpL solves the problem of relatively poor catalytic efficiency and thermal stability of existing acylhomoserine lactonase, and promotes its wider application in various fields, playing a vital role in effective quorum quenching strategies. The method for preparing a hexameric modification of acylhomoserine lactonase of the present invention replaces the amino acid sequence involved in inter-subunit interaction in other non-hexameric AHLases with the amino acid sequence involved in inter-subunit interaction in PpL, hexamerizes the non-hexameric AHLase, and obtains a hexameric modification of acylhomoserine lactonase, which also has strong thermal stability.

The present invention discloses a mutant of N-acyl homoserine lactonase and its application. Through rational design and site-directed mutagenesis techniques, the corresponding mutant is obtained, which can greatly improve the thermal stability and AHL hydrolysis activity of AHL-lactonase AhlX. After transformation, the T m value of mutant M41 is increased by 12.13 °C, and the enzyme activity is 17.67 times that of the wild type. The mutant M41 is applied to the soft rot diseases of potatoes, zucchinis, green peppers, radishes, Chinese cabbages and eggplants caused by E. carotovora infection. The results show that the soft rot lesion areas of the co-treatment groups of mutant M41 and E. carotovora are significantly lower than those of the E. carotovora treatment group. The mutant M41 can effectively alleviate various plant soft rot diseases caused by E. carotovora.

The present invention discloses a highly thermostable mutant of AHL-lactonase and its application in biological control of fire blight of fruit trees. The mutation site of the mutant is to mutate the amino acid sequence from position 176 to position 181 of the AHL-lactonase shown in SEQ ID NO.3 from the amino terminus to the carboxyl terminus. The mutated amino acid sequence is shown in SEQ ID NO.1, denoted as mutant A19-9, or to mutate the amino acid sequence from position 176 to position 184 from the amino terminus to the carboxyl terminus. The mutated amino acid sequence is shown in SEQ ID NO.2, denoted as mutant C13-1. The thermostability of both mutants has been greatly improved compared to the wild type. When the two mutants are applied to prepare a biological pathogenic bacteria inhibitor, the inhibitory Erwinia amylovora effect on fire blight of apple caused by infection is better than that of the wild type, and it provides a potential biological control agent for E.amylovora fire blight of fruit trees caused by infection.

The present invention discloses the application of a zearalenone-degrading fusion enzyme in corn by-products, belonging to the fields of microorganisms and food safety. The present invention provides a zearalenone lactonohydrolase fusion enzyme S1v1-GS-ZHD518, and the activity, expression level and thermal stability of ZHD518 lactonase are improved by fusing the S1v1 peptide. The present invention applies the fusion enzyme S1v1-GS-ZHD518 to degrade zearalenone in positive samples. This enzyme can adapt to the high-temperature conditions in the processing environment and can efficiently degrade zearalenone. The degradation rates of ZEN in corn bran, corn germ meal and corn protein powder within 1 h are 90.975%, 91.004% and 95.32% respectively, showing great application prospects.

The invention relates to the technical field of high polymer materials, and discloses an innovative preparation method of an environment-friendly degradable acrylic sheet material, the sheet material is prepared from methyl methacrylate, 2-hydroxyethyl methacrylate, autocatalytic precursor functionalized furfural derivative lactone, an enzyme response type diene cross-linking agent, 1, 4-benzene diboronic acid and other raw materials. The preparation method comprises three steps of prepolymer syrup preparation, pouring and dynamic network construction, and final curing and annealing treatment, and an acetal structure sensitive to acid and a peptide bond structure sensitive to biological enzyme are introduced into a polymer network, so that the material has dual controllable degradation characteristics of autocatalytic chemical degradation and biological enzyme degradation at the same time; and by constructing a dynamic reversible boric acid ester cross-linked network, the material is endowed with excellent thermal remodeling and damage self-repairing capabilities. The board prepared by the invention solves the problem that the traditional acrylic material is difficult to degrade and cannot be repaired and reused after being discarded on the basis of retaining good mechanical properties.

The invention relates to a genetically engineered bacterium for synthesizing L-theanine by using D-xylose. The genetically engineered bacterium is a recombinant host bacterium containing a heterologous expression Weimberg metabolic pathway related gene, a glutamate dehydrogenase encoding gene gdhA and a gamma-glutamyl methylamine synthetase encoding gene GMAS. Wherein the Weimberg metabolic pathway related genes comprise a xylose dehydrogenase encoding gene xylB, a xylose lactonase encoding gene xylC, a 2-keto-3-xylan dehydratase encoding gene yjhG, a D-KDX dehydratase encoding gene xylX and a 2, 5-dioxovalerate dehydrogenase encoding gene xylA, and the host bacteria are escherichia coli. The strain realizes efficient biosynthesis of L-theanine from D-xylose through a non-phosphorylated xylose assimilation pathway, and the pathway only needs seven enzymatic steps to complete de novo synthesis of L-theanine, is the shortest synthesis pathway known at present, and has a wide resource utilization prospect.

The invention provides a hydrothermal-source quorum sensing quenching strain Stone spring sea-source bacterium and application thereof, and the screened strain can inhibit pathogenic bacteria and is used for preparing antibacterial drugs; the preservation number of the screened stone spring sea source bacteria is CCTCC (China Center For Type Culture Collection) NO: M 20251224. The invention also provides an AHL degrading enzyme Aii937 protein separated from the YESM3 strain, and the AHL degrading enzyme Aii937 protein is characterized in that the amino acid sequence of the protein is SEQ ID NO: 2. According to the YESM3 strain disclosed by the invention, a QS channel of pathogenic bacteria can be blocked through AHL lactonase Aii937, and secretion of virulence factors of the pathogenic bacteria is reduced; the strain YESM3 has no pathogenicity. The AHL lactonase does not have broad spectrum, is high in substrate specificity, and only degrades C6-HSL and 3OC-C14-HSL.

The invention belongs to the technical field of nano-enzymes and anti-biofilm materials, and discloses a zinc-based monatomic nano-enzyme with multi-enzyme activity as well as a preparation method and application of the zinc-based monatomic nano-enzyme. The preparation method comprises the following steps: by taking imidazole-2-formaldehyde and zinc salt as precursors, synthesizing ZIF-90 through a solvothermal method; and performing pyrolysis in an inert atmosphere to obtain the Zn-Nx-C monatomic nano-enzyme. The method disclosed by the invention is simple and efficient in process, the obtained Zn-Nx-C monatomic nano-enzyme has peroxidase-like activity and homoserine lactonase-like activity, the efficiency bottleneck of a traditional single-enzyme system is broken through due to the dual-function synergistic effect, and the Zn-Nx-C monatomic nano-enzyme is suitable for the scenes of food preservation, medical instrument antibiosis, environmental microorganism control and the like and has a wide application prospect. And an efficient solution is provided for biofilm prevention and treatment.

The present invention discloses a recombinant strain of N-acyl homoserine lactonase AIO6 without a resistance screening marker and its application. The present invention relates to a recombinant strain of N-acyl homoserine lactonase AIO6 without a resistance screening marker and its application in the technical field of microorganisms and enzymes. The method for constructing a recombinant Escherichia coli without a resistance screening marker includes introducing the coding gene of N-acyl homoserine lactonase AIO6 into the recipient Escherichia coli, and then performing screening without an antibiotic resistance marker to obtain the recombinant Escherichia coli. The construction of the recombinant strain of N-acyl homoserine lactonase AIO6 without a resistance screening marker greatly improves the application of AIO6 in production, and compared with the reported AIO6 strains, the N-acyl homoserine lactonase activity of this strain is further enhanced.

The present invention discloses a method for inhibiting the viscous expansion of biochemical sludge, wherein the screened strain Rhodococcus dielectricus ( Rhodococcus electrodiphilus )TN‑3; The lactonase secreted by the dielectric Rhodococcus can degrade the acylhomoserine lactone secreted by the activated sludge that causes viscous expansion, thereby inhibiting the viscous expansion of the activated sludge; caprolactone is used as a promoter, and a certain concentration of caprolactone can promote the secretion of dielectric Rhodococcus lactonase, thereby achieving better results in inhibiting the viscous expansion of the activated sludge. The present invention can effectively solve the problem of viscous expansion of sludge in the biochemical system, is simple to operate, quick to take effect, low in cost, has practical application value, and broad market prospects, and provides a new and effective way to inhibit the viscous expansion of system sludge.

This invention provides a method derived from Bacillus ( Bacillus The lactone enzyme AiiA (sp.) GB-1 and its application are described, with its amino acid sequence shown in SEQ ID NO:1. Experimental results show that the enzyme has degradation activity against various N-acyl homoserine lactone signaling molecules, exhibits high degradation ability against long-chain AHLs, and has good stability within a certain temperature and pH range. Further experimental results show that the AiiA enzyme can reduce the quorum sensing-related phenotype of pathogens. Simultaneously, exogenous addition of AiiA enzyme can also reduce the degree of pathogen infection in plant tissues, indicating that the enzyme can be used for plant disease control through engineered bacteria expression or exogenous enzyme preparations. This invention provides a new technical approach for green control of plant diseases.

The invention discloses a sporophyte fertile gene OsSTRL3 and application thereof. A protein encoded by the sporophyte fertile gene is an amino acid sequence as shown in SEQ ID NO.1, or a protein which is subjected to substitution, deletion or addition of at least one amino acid and has the same function as the OsSTRL3; the nucleotide sequence of an encoding region of the OsSTRL3 gene is shown as SEQ ID NO.2. The invention also discloses a mutation site of the OsSTRL3. The OsSTRL3 gene disclosed by the invention encodes a protease with an isosessile synthetase / phospholactonase structural domain, and has a function of regulating and controlling normal development of pollen, sporophytic male sterility can be generated by mutation of the gene, and the mutation of the OsSTRL3 gene can be used for regulating and controlling normal development of pollen. The strain can be used as a female parent for producing hybrids in heterosis utilization.

The invention belongs to the technical field of preparation of nano biological materials, and discloses a bioactive glass nano enzyme with lactonase catalytic performance as well as a preparation method and application of the bioactive glass nano enzyme. The preparation method comprises the following steps: carrying out coordination reaction on 4-imidazole formaldehyde and zinc-doped bioactive glass prepared by a microemulsion method, and carrying out heat treatment to obtain the bioactive glass nano-enzyme with lactonase-like activity. The bioactive glass nano-enzyme obtained by the invention has a Zn-N coordination structure similar to a natural lactonase catalytic activity center, not only has good biocompatibility and intrinsic osteoinductivity of bioactive glass, but also can specifically hydrolyze bacterial quorum sensing signal molecules and efficiently inhibit the formation of a biological membrane, so that the bioactivity of the bioactive glass nano-enzyme is improved. The antibacterial-osteogenesis dual synergistic effect is achieved, and the antibacterial-osteogenesis composite material has important application value in the fields of infectious bone defect repair, periodontitis and other disease treatment.

The present invention relates to oral care compositions comprising a peptidase or lactonase, preferably a peptidase and a lactonase, the use of said compositions in medicine, the use of said compositions in the treatment of oral diseases, methods of treatment comprising the administration of said compositions to a human subject, methods of preventing or removing oral biofilm comprising contacting an oral biofilm with said compositions, methods for reducing the risk of oral biofilm formation, and kits comprising said compositions.

The invention discloses an AHL-lactonase high thermal stability mutant and application thereof in biological control of fruit tree fire blight, the mutation site of the mutant is obtained by mutating the amino acid sequence of AHL-lactonase as shown in SEQ ID NO.3 from the 176th amino acid sequence to the 181th amino acid sequence from the amino terminal to the carboxyl terminal, the mutated amino acid sequence is as shown in SEQ ID NO.1, the mutant is marked as a mutant A19-9, and the mutant A19-9 has a nucleotide sequence shown in SEQ ID NO.1. The amino acid sequence from the amino terminal to the carboxyl terminal is mutated, or the amino acid sequence from the 176th site to the 184th site from the amino terminal to the carboxyl terminal is mutated, the mutated amino acid sequence is shown as SEQ ID NO.2 and is marked as the mutant C13-1, and the thermal stability of the two mutants is greatly improved compared with that of the wild type. The two mutants are applied to preparation of a biological pathogenic bacterium inhibitor, the effect of inhibiting apple fire blight caused by Erwinia amylovora infection is better than that of a wild type, and a potential biological control agent is provided for fruit tree fire blight caused by Erwinia amylovora infection.

This invention discloses a method derived from Bacillus ( Bacillus The population quenching lactonease YtnP gene of sp.) GB-1, the nucleotide sequence of which is shown in SEQ ID NO:1, was introduced into the soft rot pathogen via a two-parent hybridization method. Heterologous overexpression of this gene significantly inhibited biofilm formation, motility, and host pathogenicity of the soft rot pathogen. It was also transferred into Arabidopsis thaliana via Agrobacterium-mediated transformation and overexpressed in Arabidopsis. ytnP Genes can significantly enhance plant resistance to soft rot, and this invention provides new resources and technical approaches for the prevention and control of soft rot.

The invention relates to the technical field of agricultural biology, and discloses a method for regulating and controlling a rhizosphere microbial community structure based on a tea tree, and the core of the method is to prepare and apply a composite regulation and control preparation. The preparation method comprises the following steps: firstly, covalently immobilizing N-acyl homoserine lactonase on the surface of a mesoporous biochar carrier so as to intervene pathogenic bacteria quorum sensing; secondly, loading the plant system elicitor and the synergistic nutritional agent into the carrier through vacuum impregnation; and finally, coating a layer of pH-sensitive hydrogel composed of chitosan and sodium alginate on the outside through an ionic cross-linking method. The preparation structure ensures the long-term stability of surface enzyme, and meanwhile, the external hydrogel layer can respond to a local low-pH environment caused by root exudates of tea trees to trigger accurate release of internal active substances in a root zone. The method can effectively regulate and control rhizosphere microorganism balance, synergistically induce tea tree resistance and promote tea quality substance synthesis, and has the beneficial effects of high targeting property and high utilization rate.

The invention discloses AHL lactonase NJ1 from pathogenic bacillus as well as a preparation method and application of the AHL lactonase NJ1. The invention belongs to the technical field of biology, and particularly relates to AHL lactonase NJ1 derived from pathogenic bacteria as well as a preparation method and application of the AHL lactonase NJ1. The invention provides application of quenching enzyme NJ1 in preparation of an aquatic disease inhibitor. The quenching enzyme NJ1 is applied to preparation of a product for preventing or treating diseases caused by aeromonas veronii infection; the quenching enzyme NJ1 is applied to inhibition of pathogenic bacteria biofilm formation; the application of the quenching enzyme NJ1 in inhibiting the hemolysis capability of pathogenic bacteria or the activity of extracellular protease; the quenching enzyme NJ1 is applied to aquaculture. The quenching enzyme NJ1 has the capability of efficiently degrading AHL, can keep the activity for a long time in an aquaculture environment, and has application value.