13 results about "Lactonase" patented technology

According to the scheme, the invention provides the recombinant macrolide enzyme as well as the preparation method and the application thereof, the amino acid sequence of the recombinant macrolide enzyme is as shown in SEQ ID NO.1, and the recombinant macrolide enzyme is derived from an EreC esterase family of enterobacter hormaechei and is obtained through site-specific mutagenesis; the mutation sites are as follows: glutamic acid at the 44th site is mutated into asparagine, tyrosine at the 55th site is mutated into proline, proline at the 76th site is mutated into arginine, phenylalanine at the 153rd site is mutated into alanine, and serine at the 219th site is mutated into glutamic acid. The method is expected to remove macrolide antibiotic pollution, and has good economic benefits and practical values.

According to the scheme, the recombinant macrolide enzyme and the preparation method and application thereof are provided, the amino acid sequence of the recombinant macrolide enzyme is shown as SEQ ID NO.1, and the recombinant macrolide enzyme is obtained by site-directed mutagenesis of an erythromycin esterase family protein sequence derived from enterobacter hormaechei; the mutation sites are as follows: the 44 glutamic acid is mutated into asparagine, the 55 tyrosine is mutated into proline, the 153 proline is mutated into alanine, and the 219 serine is mutated into glutamic acid, so that the specific macrolide lactonase which is efficient, stable, easy to prepare on a large scale and more suitable for environmental requirements is prepared, and macrolide antibiotic pollution is removed; and the method has good economic benefits and practical values.

The application provides a hot liquid source quorum quenching strain Shiquan Haeyuan bacteria and application thereof, the strain screened can inhibit pathogenic bacteria and is used for preparing bacteriostatic drugs; the preservation number of the Shiquan Haeyuan bacteria screened is CCTCC NO: M 20251224.The application also provides an AHL degradation enzyme Aii937 protein separated from the YESM3 strain, characterized in that the amino acid sequence of the protein is SEQ ID NO: 2.The YESM3 strain of the application can block the QS path of pathogenic bacteria through AHL lactonase Aii937, reduce the secretion of virulence factors of pathogenic bacteria; the strain YESM3 has no pathogenicity.AHL lactonase does not have broad spectrum, has strong substrate specificity, and only degrades C6-HSL and 3OC-C 14 -HSL.

The invention relates to the technical field of high polymer materials, and discloses an innovative preparation method of an environment-friendly degradable acrylic sheet material, the sheet material is prepared from methyl methacrylate, 2-hydroxyethyl methacrylate, autocatalytic precursor functionalized furfural derivative lactone, an enzyme response type diene cross-linking agent, 1, 4-benzene diboronic acid and other raw materials. The preparation method comprises three steps of prepolymer syrup preparation, pouring and dynamic network construction, and final curing and annealing treatment, and an acetal structure sensitive to acid and a peptide bond structure sensitive to biological enzyme are introduced into a polymer network, so that the material has dual controllable degradation characteristics of autocatalytic chemical degradation and biological enzyme degradation at the same time; and by constructing a dynamic reversible boric acid ester cross-linked network, the material is endowed with excellent thermal remodeling and damage self-repairing capabilities. The board prepared by the invention solves the problem that the traditional acrylic material is difficult to degrade and cannot be repaired and reused after being discarded on the basis of retaining good mechanical properties.

The invention relates to a genetically engineered bacterium for synthesizing L-theanine by using D-xylose. The genetically engineered bacterium is a recombinant host bacterium containing a heterologous expression Weimberg metabolic pathway related gene, a glutamate dehydrogenase encoding gene gdhA and a gamma-glutamyl methylamine synthetase encoding gene GMAS. Wherein the Weimberg metabolic pathway related genes comprise a xylose dehydrogenase encoding gene xylB, a xylose lactonase encoding gene xylC, a 2-keto-3-xylan dehydratase encoding gene yjhG, a D-KDX dehydratase encoding gene xylX and a 2, 5-dioxovalerate dehydrogenase encoding gene xylA, and the host bacteria are escherichia coli. The strain realizes efficient biosynthesis of L-theanine from D-xylose through a non-phosphorylated xylose assimilation pathway, and the pathway only needs seven enzymatic steps to complete de novo synthesis of L-theanine, is the shortest synthesis pathway known at present, and has a wide resource utilization prospect.

The present invention discloses a method for inhibiting the viscous expansion of biochemical sludge, wherein the screened strain Rhodococcus dielectricus ( Rhodococcus electrodiphilus )TN‑3; The lactonase secreted by the dielectric Rhodococcus can degrade the acylhomoserine lactone secreted by the activated sludge that causes viscous expansion, thereby inhibiting the viscous expansion of the activated sludge; caprolactone is used as a promoter, and a certain concentration of caprolactone can promote the secretion of dielectric Rhodococcus lactonase, thereby achieving better results in inhibiting the viscous expansion of the activated sludge. The present invention can effectively solve the problem of viscous expansion of sludge in the biochemical system, is simple to operate, quick to take effect, low in cost, has practical application value, and broad market prospects, and provides a new and effective way to inhibit the viscous expansion of system sludge.

This invention provides a method derived from Bacillus ( Bacillus The lactone enzyme AiiA (sp.) GB-1 and its application are described, with its amino acid sequence shown in SEQ ID NO:1. Experimental results show that the enzyme has degradation activity against various N-acyl homoserine lactone signaling molecules, exhibits high degradation ability against long-chain AHLs, and has good stability within a certain temperature and pH range. Further experimental results show that the AiiA enzyme can reduce the quorum sensing-related phenotype of pathogens. Simultaneously, exogenous addition of AiiA enzyme can also reduce the degree of pathogen infection in plant tissues, indicating that the enzyme can be used for plant disease control through engineered bacteria expression or exogenous enzyme preparations. This invention provides a new technical approach for green control of plant diseases.

The invention discloses a sporophyte fertile gene OsSTRL3 and application thereof. A protein encoded by the sporophyte fertile gene is an amino acid sequence as shown in SEQ ID NO.1, or a protein which is subjected to substitution, deletion or addition of at least one amino acid and has the same function as the OsSTRL3; the nucleotide sequence of an encoding region of the OsSTRL3 gene is shown as SEQ ID NO.2. The invention also discloses a mutation site of the OsSTRL3. The OsSTRL3 gene disclosed by the invention encodes a protease with an isosessile synthetase / phospholactonase structural domain, and has a function of regulating and controlling normal development of pollen, sporophytic male sterility can be generated by mutation of the gene, and the mutation of the OsSTRL3 gene can be used for regulating and controlling normal development of pollen. The strain can be used as a female parent for producing hybrids in heterosis utilization.

The invention belongs to the technical field of preparation of nano biological materials, and discloses a bioactive glass nano enzyme with lactonase catalytic performance as well as a preparation method and application of the bioactive glass nano enzyme. The preparation method comprises the following steps: carrying out coordination reaction on 4-imidazole formaldehyde and zinc-doped bioactive glass prepared by a microemulsion method, and carrying out heat treatment to obtain the bioactive glass nano-enzyme with lactonase-like activity. The bioactive glass nano-enzyme obtained by the invention has a Zn-N coordination structure similar to a natural lactonase catalytic activity center, not only has good biocompatibility and intrinsic osteoinductivity of bioactive glass, but also can specifically hydrolyze bacterial quorum sensing signal molecules and efficiently inhibit the formation of a biological membrane, so that the bioactivity of the bioactive glass nano-enzyme is improved. The antibacterial-osteogenesis dual synergistic effect is achieved, and the antibacterial-osteogenesis composite material has important application value in the fields of infectious bone defect repair, periodontitis and other disease treatment.

The present invention relates to oral care compositions comprising a peptidase or lactonase, preferably a peptidase and a lactonase, the use of said compositions in medicine, the use of said compositions in the treatment of oral diseases, methods of treatment comprising the administration of said compositions to a human subject, methods of preventing or removing oral biofilm comprising contacting an oral biofilm with said compositions, methods for reducing the risk of oral biofilm formation, and kits comprising said compositions.

This invention discloses a method derived from Bacillus ( Bacillus The population quenching lactonease YtnP gene of sp.) GB-1, the nucleotide sequence of which is shown in SEQ ID NO:1, was introduced into the soft rot pathogen via a two-parent hybridization method. Heterologous overexpression of this gene significantly inhibited biofilm formation, motility, and host pathogenicity of the soft rot pathogen. It was also transferred into Arabidopsis thaliana via Agrobacterium-mediated transformation and overexpressed in Arabidopsis. ytnP Genes can significantly enhance plant resistance to soft rot, and this invention provides new resources and technical approaches for the prevention and control of soft rot.

The invention relates to the technical field of agricultural biology, and discloses a method for regulating and controlling a rhizosphere microbial community structure based on a tea tree, and the core of the method is to prepare and apply a composite regulation and control preparation. The preparation method comprises the following steps: firstly, covalently immobilizing N-acyl homoserine lactonase on the surface of a mesoporous biochar carrier so as to intervene pathogenic bacteria quorum sensing; secondly, loading the plant system elicitor and the synergistic nutritional agent into the carrier through vacuum impregnation; and finally, coating a layer of pH-sensitive hydrogel composed of chitosan and sodium alginate on the outside through an ionic cross-linking method. The preparation structure ensures the long-term stability of surface enzyme, and meanwhile, the external hydrogel layer can respond to a local low-pH environment caused by root exudates of tea trees to trigger accurate release of internal active substances in a root zone. The method can effectively regulate and control rhizosphere microorganism balance, synergistically induce tea tree resistance and promote tea quality substance synthesis, and has the beneficial effects of high targeting property and high utilization rate.