48 results about "[Candida] apicola" patented technology

The invention provides a diglyceride oil composition for reducing lactone as well as a product and a preparation method thereof, and relates to the technical field of functional grease processing. The preparation method of the diglyceride oil composition comprises the following steps: (1) mixing corn oil and high oleic acid peanut oil, adding glycerol and water, then adding rhizomucor miehei lipase and pseudomonas onions lipase, and reacting to obtain a material A; (2) mixing sea buckthorn seed oil, acer truncatum seed oil and rapeseed oil, adding glycerol and water, adding candida antarctica lipase B and rhizopus oryzae lipase, and reacting to obtain a material B; and (3) mixing the material A and the material B, heating and centrifuging, taking an upper-layer oil sample, and distilling under a vacuum condition to obtain the diglyceride oil composition. The diglyceride oil composition prepared by the invention has a good effect in the aspect of reducing the internal fat.

The invention discloses a bacterium-enzyme synergistic fermentation method for sweet potato residues, which can realize rapid fermentation and has high protein content of the fermented sweet potato residues. According to the method, high-temperature-resistant alpha-amylase, cellulase and pectinase are used for carrying out enzymolysis on the sweet potato residues to decompose the sweet potato residues into reducing sugar, cellulose is preliminarily decomposed, and rapid and accurate starch conversion and cellulose degradation are carried out; a multi-bacterium combination of efficient degradation fibers, candida utilis capable of producing mycoprotein, lactobacillus plantarum JM113, bacillus subtilis and aspergillus niger is used for fermentation, so that the mycoprotein level in the fermented sweet potato residues is further improved, the nutritional value and the in-vitro digestibility are improved, the feeding price of the fermented sweet potato residues is improved, and the cost problem caused by high-moisture treatment is reduced; therefore, the economic value of the sweet potato residues is comprehensively improved, and the method has practical significance in waste utilization of the sweet potato residues, reduction of protein feed cost, alleviation of waste residue environmental pollution and improvement of industrial economic benefits. The method is suitable for popularization and application in the technical field of biological fermentation.

The invention relates to a protein feed prepared from biologically refined distiller's grains and a preparation method thereof, and relates to the technical field of bioengineering and feed resource development. The method comprises the following steps: (1) sequentially carrying out acid pretreatment and double-enzyme synergistic hydrolysis on the white spirit vinasse to obtain hydrolysate containing fermentable sugar; (2) adding a candida utilis yeast liquid and urea, and carrying out carbon-nitrogen synergistic liquid fermentation to obtain a fermentation liquid with the viable count of candida utilis greater than or equal to 4.0 * 10 < 8 > CFU / mL; and drying. According to the method, the white spirit vinasse is subjected to diluted acid high-pressure pretreatment and double-enzyme synergistic hydrolysis, then candida utilis is utilized for carbon-nitrogen synergistic liquid fermentation, efficient utilization of glucose and xylose is achieved, and the mycoprotein yield is larger than or equal to 0.70 g / L / h; according to the method, all-component high-value utilization of the distiller's grains is realized, and a process path with industrial production potential is provided for preparing the protein feed by biorefining the distiller's grains.

The invention provides plant fermented oil with moistening and soothing effects and a preparation method thereof. The preparation method comprises the following steps: fermenting aspergillus in a fermentation culture medium 1, adding candida albicans, inoculating into a fermentation culture medium 2, carrying out primary fermentation under the ventilation capacity of 2-4 L / min, carrying out secondary fermentation under the ventilation capacity of 0.2-0.5 L / min, separating, and sterilizing to obtain the plant fermented oil. The aspergillus and candida mellifera are mixed and fermented according to a specific sequence, so that the obtained plant fermented oil has excellent soothing and moistening effects. Besides, compared with products obtained through fermentation in other modes, the plant fermentation oil has more excellent soothing and moistening effects and is very suitable for being used as and / or used for preparing cosmetics or medical compositions.

This invention relates to a protein feed prepared from biorefined baijiu lees and its preparation method, belonging to the fields of bioengineering and feed resource development technology. It includes the following steps: (1) subjecting the baijiu lees to acid pretreatment and dual-enzyme synergistic hydrolysis sequentially to obtain a hydrolysate containing fermentable sugars; (2) adding Candida utilis culture and urea for carbon-nitrogen synergistic liquid fermentation to obtain a Candida utilis viable count ≥4.0 × 10⁻⁶. 8 Fermentation broth with CFU / mL; drying is then performed. This invention pretreats baijiu (Chinese liquor) lees under high pressure with dilute acid, followed by synergistic hydrolysis with two enzymes, and then utilizes *Candida utilis* for carbon-nitrogen synergistic liquid fermentation, achieving efficient utilization of glucose and xylose, with a cell protein yield ≥0.70 g / L / h. This invention realizes the high-value utilization of all components of baijiu lees, providing a process route with industrial-scale production potential for the biorefining of baijiu lees to prepare protein feed.

This invention belongs to the field of alcoholic beverage processing technology, specifically relating to a high-purity ginkgo wine and its preparation method. The preparation method comprises the following steps: ginkgo slurry undergoes preliminary enzymatic hydrolysis with neutral protease and amylase, followed by secondary enzymatic hydrolysis with pectinase and sodium bisulfite; brewing yeast fermentation broth is added for pre-fermentation; glutamine transaminase, *Saccharomyces cerevisiae* fermentation broth, and *Candida valerate* fermentation broth are added for post-fermentation; and finally, distillation and filtration are performed to obtain the ginkgo wine. This invention achieves complete decomposition and fermentation of ginkgo kernels through a two-step enzymatic hydrolysis and two-step fermentation process. The preparation method has advantages such as simple process and low cost, which is beneficial for industrial production; the prepared ginkgo wine has advantages such as clarity, strong aroma, high alcohol content, and low acidity.

The invention belongs to the technical field of microorganisms, and particularly relates to a compound fermentation inoculant for increasing the relative content of ethyl decanoate in roxburgh rose fruit wine and application. According to the present invention, two saccharomycetes are separated from a wild rosa roxburghii fermentation broth, i.e., a Starmerella bacillus and a Saccharomyces cerevisiae, and are compounded to obtain the composite fermentation bacterial agent, and the bacterial agent can produce ethyl decanoate at high yield and is applied to rosa roxburghii fruit wine brewing, such that the relative content of ethyl decanoate in the rosa roxburghii fruit wine is increased, and the yield of the rosa roxburghii fruit wine is increased; the flavor quality of the roxburgh rose fruit wine is improved, and technical support is provided for industrial development of the roxburgh rose fruit wine. When the composite microbial agent is used for fermenting the rosa roxburghii tratt fruit wine, the flavor and taste of the rosa roxburghii tratt fruit wine can be remarkably improved, the relative content of ester substances in volatile flavor substances of the rosa roxburghii tratt fruit wine is increased by 68.527% in total, and the highest relative content of ethyl decanoate is 26.551%. In addition, the relative content of ethyl laurate and the relative content of ethyl caprylate are increased to 14.228% and 11.335% respectively.

The present disclosure provides a method for producing chemicals using halotolerant yeast. The method includes culturing halotolerant yeast cells in a medium comprising a solution of salts at a concentration of at least 0.5 times the salinity of seawater, and allowing the halotolerant yeast cells to produce one or more chemicals. The medium may include a carbon source, nitrogen source and / or phosphate source, trace elements, and additives. The halotolerant yeast cells may be selected from, e.g., Debaryomyces subglobosus, Candida taylori, Pichia anomaly Millerozyma farinosa, Blastobotrys adeninivorans, Scheffersomyces stipitis. or combinations thereof. The method enables chemical production in high-salt media, reducing freshwater usage in fermentation processes.

The invention relates to the technical field of microbial fermentation, in particular to a preparation method and application of a digestible biological protein fermentation material. According to the fermentation method, candida utilis serves as a fermentation strain, industrial and agricultural waste glucose mother liquor and corn steep liquor serve as substrates, high-yield mycoprotein is obtained under the relative low-temperature fermentation condition of 20-25 DEG C, and the obtained fermentation liquor is subjected to single enzymolysis, so that a fermentation material easier to digest is obtained. When weaned piglets are fed with the obtained fermented material, the diarrhea rate of the piglets can be effectively reduced.

The present invention belongs to the field of food processing technology, and discloses a kind of rice bran fresh wet noodles and preparation method thereof. According to mass percentage, the raw material components of rice bran fresh wet noodles are rice bran 25-30%, chia seed powder 3-6%, white tea powder 4-7%, wheat flour 55-65%, salt 0.5-1.5%, a first composite bacterial agent 1.5-2%, and a second composite bacterial agent 1-1.5%; wherein, the first composite bacterial agent is obtained by mixing Lactobacillus acidophilus, Lactobacillus fermentum, and Candida utilis in a mass ratio of 3-6:8-12:2-4; the second composite bacterial agent is obtained by mixing Saccharomyces cerevisiae and Lactobacillus plantarum in a mass ratio of 1-4:3-10. The rice bran fresh wet noodles of the present invention are bright in color and light yellow. Compared with the existing fresh wet noodles, the nutrient content is significantly increased. It is not easy to break and will not become mushy during cooking. It has a more delicate and smooth taste, good viscoelasticity and chewiness, and shows better quality.

The present application relates to high protein fermentation technical field, specifically disclose a kind of preparation method for fermenting production of yeast protein based on alcohol waste liquor, to solve the technical problems such as low utilization rate of alcohol waste liquor resource, feed soybean meal supply is nervous and high cost.The method includes the following steps: first, the alcohol waste liquor produced by grain deep processing is concentrated to dry basis content ≥30%, the composite carbon source carrier is crushed, urea and molasses are added to adjust the carbon-nitrogen ratio to (20~25):1, access to the complex fermentation strain containing Candida utilis, solid fermentation is carried out, and the finished product yeast protein is obtained.The present application uses solid fermentation mode, matches composite carbon source carrier to optimize nutrient ratio, step by step controls key process parameters, and the finished product yeast protein is ≥24%, true protein is ≥22%, and nitrogen conversion rate is ≥85%, which can replace soybean meal as feed additive.The method realizes alcohol waste liquor resource utilization, pollution is small, reduces feeding cost, has environmental protection and economic value, and is suitable for industrialization popularization and application.

According to the tomato pomace fermented protein feed and the preparation method thereof provided by the invention, tomato pomace and bran are used as fermentation substrates, candida utilis and pichia pastoris are used as fermentation strains, fermentation processes such as strain inoculum size, fermentation time and enzyme addition amount are further optimized, and under optimal fermentation process conditions, the tomato pomace fermented protein feed is prepared. The content of crude protein in the obtained tomato peel residues is 35.93%. After fermentation, the crude protein content of the tomato peel residues is increased by 10.59%, and hemicellulose is reduced by 4.81%. The tomato pomace is cooperatively fermented by bacteria and enzymes, so that the cellulose content of the tomato pomace is reduced, the protein content of the tomato pomace is increased, the tomato pomace can be subsequently used for feeding monogastric livestock and poultry, and the method has great significance in realizing resource utilization of wastes, reducing environmental pollution and relieving waste treatment pressure faced by tomato processing enterprises. The nutritional value of the feed can be improved, the breeding cost is reduced, and sustainable development of animal husbandry is promoted.

The present invention relates to the technical field of microbial fermentation, and discloses a method for producing selenium-rich protein feed by multi-strain co-fermentation of Hami melons. The Hami melons are crushed, then mixed with a carbon source and a nitrogen source, and then inoculated with a fermentation agent for fermentation; the fermented product is dried and pulverized to obtain a protein feed; the fermentation agent includes Candida utilis, Aspergillus niger, and Bacillus licheniformis; the components of the Candida utilis culture medium include yeast extract 9.56 g / L, glucose 19.86 g / L, dipotassium hydrogen phosphate 1.45 g / L, magnesium sulfate 1.12 g / L, sodium selenite 25 mg / L, L-lysine and vitamin B1. In terms of mass concentration, the ratio of sodium selenite, L-lysine and vitamin B1 is 1:(116-232):(0.06-0.12). In the present invention, multi-strain co-fermentation of Hami melons is adopted to obtain a selenium-rich protein feed.

The invention belongs to the crossing field of cultivation and bioengineering, and particularly relates to a multi-strain insect protein and microalgae compounded bullfrog feed and a preparation method thereof, the multi-strain insect protein and microalgae compounded bullfrog feed comprises the following components: 25-45% of fermented insect protein, 15-30% of DHA microalgae powder, 10-20% of an auxiliary protein source, 0.5-2% of compound vitamin selected from fermented soybean meal or enzymolysis cottonseed protein, 0.5-2% of vitamin B, 0.5-2% of vitamin C, 0.5-2% of vitamin C, 0.5-2% of vitamin C, 0.5 1%-3% of composite mineral substances, 2%-5% of an adhesive and the balance of carbohydrate; the total DHA content of the bullfrog feed is larger than or equal to 1.5% by dry weight, the ratio of omega-3 fatty acid to omega-6 fatty acid is maintained to be 1: 1-1: 3, and the bullfrog feed has the advantages that saccharomyces cerevisiae is replaced with candida utilis, so that the capability of decomposing insect chitin is enhanced; chlorella is used for supplementing chlorophyll and algal polysaccharides to form a cell protection synergistic effect with schizochytrium limacinum, and DHA is supplemented at the same time; under the action of alkaline protease secreted by bacillus subtilis, the proportion of a beta-folding structure is reduced from 32% to 18%, and the digestibility is remarkably improved.

The invention provides a method for preparing ruminant biological feed through fermentation of sodium humate and probiotics, and relates to the field of solid feed fermention.The method comprises the steps that S1, candida utilis powder, lactobacillus plantarum liquid, enterococcus faecium liquid and beet molasses are stirred and mixed, and molasses bacterial liquid is obtained; s2, mixing the molasses bacterial liquid with the treated sodium humate and gelatinized starch urea according to a preparation ratio, and uniformly stirring; and S3, loading into an anaerobic sealed fermentation bag, and naturally fermenting in a greenhouse at the temperature of 25-35 DEG C for 30-48 hours. The biological feed which is rich in high-activity probiotics, high in protein and easy to digest and absorb by animals is prepared from byproducts such as sodium humate, molasses and the like by utilizing biological engineering, and can replace antibiotics in the feed.

The application provides a vinegar residue-based single-cell protein feed and a preparation method and application thereof, and belongs to the technical field of feed preparation.The application uses vinegar residue as raw material, adopts solid-state fermentation of Aspergillus, Candida utilis and Saccharomyces cerevisiae in combination with composite enzymes, utilizes the synergistic effect among the strains and the enzymolysis effect of the exogenous enzymes, efficiently degrades the fibrous substances in the vinegar residue, and converts the fibrous substances into microbial proteins, so that the crude protein content of the prepared vinegar residue-based single-cell protein feed is increased from 5% to 8% of the raw material to 20% to 25%, and the total fibrous substance degradation rate is greater than or equal to 60%. The vinegar residue-based single-cell protein feed produced by the application has good feeding value, can effectively replace part of the protein raw materials such as soybean meal, improves the production performance of beef cattle, reduces the breeding cost, realizes high-value utilization of vinegar residue resources, and has good popularization and application prospect.

The invention discloses a formula of a fermented feed for enhancing animal immune function, and relates to the technical field of fermented feeds. The formula comprises the following components in parts by mass: a basic raw material, an immune enhancement extract, a functional additive, 0.3-0.6 part of a composite microbial agent and 2-4 parts of composite mineral substances. Wherein the ganoderma lucidum extract and the folium cortex eucommiae extract activate immune cells through the synergistic effect of polysaccharide and chlorogenic acid; the complex microbial inoculant is composed of lactobacillus acidophilus, bacillus licheniformis and candida utilis according to a ratio of (5-6): (3-4): 2, efficiently degrades antinutritional factors and generates immunocompetence metabolites. The preparation process comprises the following steps: supercritical extraction of plant active components, mixing and humidifying of base materials, segmented stirring and fermentation after microbial inoculum activation, and then addition of functional components and drying. Through synergistic combination of the raw materials and process optimization, the animal immunity is remarkably improved, and the feed additive has the advantages of low cost and multiple immune enhancement mechanisms.

The invention relates to the field of livestock and poultry breeding, and discloses a laying hen feed additive based on fermented broussonetia papyrifera and application of the laying hen feed additive. The additive is prepared by taking paper mulberry leaves as a main material, matching with rice husks, wheat bran and other auxiliary materials, inoculating a microecological preparation containing lactobacillus plantarum, candida utilis and bacillus subtilis, and performing anaerobic fermentation. 4% of the additive is used for daily ration of the aged laying hens, so that the laying rate can be remarkably increased, the dirty egg rate can be reduced, and the egg weight, the eggshell strength and the yolk color can be improved; the mechanism is to improve the form of the intestinal tract, enhance the expression of tight junction protein, regulate immune balance, optimize the structure of cecum flora and enrich beneficial microorganisms; the broussonetia papyrifera leaf feed additive realizes efficient utilization of broussonetia papyrifera leaf resources, provides a safe and effective functional additive for prolonging the laying period of laying hens and improving the breeding benefits, and has a good application prospect.

The invention discloses a preparation method and application of a rice fermentation product, and the preparation method comprises the following steps: S1, mixing rice, cold water and glucose according to a weight ratio of 1: (15-25): (0.01-0.03), soaking for 8 hours, and performing autoclaved sterilization at 100-121 DEG C for 10-20 minutes; s2, a composite yeast seed solution is inoculated into the sterilized rice mixed solution, the composite yeast seed solution is composed of candida utilis and Michelia yeast according to the weight ratio of (1-2): 1, and the weight ratio of rice to the composite yeast seed solution is (20-30): 1; s3, fermenting for 24-72 hours under the conditions of non-sealing, constant temperature of 26-30 DEG C and oscillation speed of 150 rpm / min, and controlling the pH value to be 4.5-6.0; s4, performing high-pressure microjet homogenization on the fermentation product at the rotating speed of 6-10rpm for 10-20 minutes; and S5, centrifuging the homogenized liquid for 30 minutes at 4000 rpm, collecting supernate, sterilizing for 20 minutes at 120 DEG C, filtering by using a microporous membrane of 0.45 mu m, and adding p-hydroxyacetophenone which accounts for 0.3-0.5% of the total weight of the filtrate and 1, 2-hexanediol which accounts for 0.5-1% of the total weight of the filtrate.

The present application relates to a protein feed containing a composite fermentation composition, and belongs to the technical field of microbial fermentation and feed.The protein feed comprises fermentation strains and fermentation substrates; the fermentation strains comprise Rhodopseudomonas palustris with a preservation number of CGMCC NO.17021 and Candida glabrata with a preservation number of CCTCC NO:M2015664; and the fermentation substrates comprise cottonseed meal powder, rapeseed meal powder, distiller's grains powder and citrus dreg powder.The protein feed containing the composite fermentation composition has a high feed utilization rate and can improve the immunity of the animals fed with the protein feed.

The invention relates to a preparation method of a cabbage byproduct solid-state fermentation biological feed, which comprises the following steps: respectively using lactic acid bacteria and candida utilis as fermentation strains, and using crushed and sterilized cabbage rotten vegetable as a culture medium to carry out solid-state fermentation to produce a protein feed. Through a single factor test, the fermentation time, the temperature and the strain inoculum size are optimized by taking the protein content as an index, and the optimal fermentation condition for the solid-state fermentation of the cabbage leftovers is selected. Initially, the lactobacillus fermentation time is 60 hours, the fermentation temperature is 30 DEG C, and the inoculation amount is 5%; the fermentation time of the candida utilis is 75 hours, the fermentation temperature is 30 DEG C, and the inoculation amount is 2%. Compared with a conventional fermentation mode, the solid-state fermentation has the advantages that microbial fermentation is carried out under the condition that the free water content is extremely low in the whole process, and the solid-state fermentation has the advantages of being fast in microbial growth, high in enzyme activity, low in cost, low in energy consumption, convenient to operate and the like.

The invention discloses a method for improving the quality of sugarcane tip silage through lactic acid bacteria-saccharomycetes synergistic fermentation. The method comprises the following steps: (1) preparing silage raw materials; (2) preparing bacterial suspension of lactic acid bacteria and saccharomycetes; and (3) preparing and storing the silage. According to the method, based on the high-sugar raw material characteristic of sugar cane tips, lactic acid bacteria and saccharomycetes are added to synergistically regulate and control the quality of the sugar cane tip silage, and lactic acid bacteria (lactobacillus or lactococcus and leuconostoc) and saccharomycetes (candida boidinii and pichia kudriavzevii) are used for synergistically anaerobic fermentation in the silage process, so that the quality of the sugar cane tip silage is improved. The substrate sugar is decomposed to generate a large amount of lactic acid, and the pH value of the feed is effectively reduced, so that the growth of harmful microorganisms is inhibited, the generation of ethanol is reduced, more nutritional ingredients are retained, and the quality of the sugar cane tip silage is further improved.

The invention relates to a fermented Chinese pulsatilla root preparation for improving the yield of laying hens in humid and hot weather, relieving stress and improving the antioxidant function. Specifically, the invention provides application of the traditional Chinese medicine fermentation composition, and the traditional Chinese medicine fermentation composition is applied to one or more of the following groups: the traditional Chinese medicine fermentation composition is used for improving the feed conversion rate of poultry; the morbidity and mortality of poultry are reduced; the composition is used for reducing the hormone level related to high-temperature and high-humidity stress of poultry; the preparation method of the fermented Chinese pulsatilla root composition comprises the following steps: carrying out expanding culture on bacillus subtilis, lactobacillus plantarum and candida utilis to obtain compound probiotic liquid, and fermenting Chinese pulsatilla root powder by using the compound probiotic liquid to obtain the fermented composition. The feed has the advantages of solving the prominent problems of reduced immunity, high incidence of pathogenic bacteria infection, reduced production performance and the like caused by environmental stress in laying hen breeding in southern hot and humid areas, so as to improve the economic value of poultry production in summer.

The invention discloses a preparation method of a honey, royal jelly and propolis fermentation composition, the fermentation composition and application. The preparation method provided by the invention comprises the following steps: adding a first-stage substrate into a basic fermentation culture medium, inoculating candida albicans and kluyveromyces marxianus, and performing first-stage fermentation to obtain first fermentation liquor; supplementing a second-stage substrate into the first fermentation liquor, and carrying out second-stage fermentation to obtain second fermentation liquor; and performing cell disruption and post-treatment on the second fermentation liquid to obtain the honey, royal jelly and propolis fermentation composition. The candida albicans and the kluyveromyces marxianus are adopted to conduct two-stage synergistic fermentation on the three substrates of the honey, the royal jelly and the propolis, cell disruption is conducted after synergistic fermentation, yeast lysate is released, and the prepared fermentation composition is clear and transparent in appearance and good in stability, has the rich rose fragrance, and is suitable for being used as a raw material. And the capacity of inhibiting inflammatory factors and promoting collagen synthesis is remarkable.

The present invention relates to a composition with anti-aging efficacy and its application. The hydrolyzed Candida strain after activation culture is inoculated into a mixed oil of Alnus rhombifolia seed oil and the fermentation product of Candida apicola / glucose / methyl rapeseedate, and fermentation is carried out to obtain a preliminary fermentation product; the obtained preliminary fermentation product is centrifuged and filtered to obtain a composite fermentation product containing various metabolites. The composition with anti-aging efficacy includes the composite fermentation product, bakuchiol, and rice fermentation broth, and the weight ratio of the composite fermentation product, bakuchiol, and rice fermentation broth is 1-3:1-2:1-3. Compared with the prior art, the present invention can form a self-assembled reverse micelle system, reducing the harm of emulsifiers to the skin. The synergistic effect of the composite fermentation product, bakuchiol, and rice fermentation broth in the composition with anti-aging efficacy jointly exerts the anti-aging efficacy.

The present application relates to a fermented water-oil double-layer composition and its preparation method and application, and belongs to the technical field of daily chemicals. The fermented water-oil double-layer composition is composed of an aqueous phase liquid and an oil phase liquid, wherein the weight ratio of the aqueous phase liquid to the oil phase liquid is 40-60:40-60; the aqueous phase liquid comprises yeast / rice fermentation product filtrate, yeast lysate extract, humectant and functional ingredients; the oil phase liquid comprises squalane, vegetable oil and fermented oil, and the fermented oil contains Candida oleaginosus / glucose / methyl oleate fermentation product, corn germ oil and glutathione. No additional water is added in the formula of the present application. Meanwhile, special oil fermentation strains are used to co-ferment with natural oil and active ingredients, so that the active ingredients are better dissolved and lysed in the oil phase, and natural fermented oil is used as the main component of the oil phase liquid. The present application has good moisturizing and penetrating properties, and can solve the greasy problem in the use feeling of pure oil phase essence oil, and the use feeling is refreshing and not greasy.

The invention discloses a composition rich in natural B vitamins as well as a preparation method and application thereof, and belongs to the field of sports nourishment raw materials. The composition is prepared by fermenting raw materials including glucose, peptone, yeast extract, magnesium sulfate and the like by adopting mixed strains including saccharomyces cerevisiae ATCC26603, candida utilis ATCC9950 and rhodotorula glutinis ATCC32765; and treating by combining an ultrahigh pressure synergistic enzyme method process to obtain yeast extract powder which is rich in natural B vitamins and is relatively high in yeast protein yield. The yeast extract prepared by the invention has excellent muscle injury protection and repair effects, and the sports nourishment prepared by compounding the yeast extract with an embedding medium and an antioxidant can reduce muscle injury after sports, promote muscle development and meet core nutritional requirements of sports crowds.

The invention discloses a Lu-type Daqu liquor yeast medicine and a preparation method thereof, and belongs to the technical field of liquor brewing. The preparation method comprises the following steps: respectively preparing mixed seed solutions from lactobacillus, bacillus aceticus and bacillus subtilis, saccharomyces cerevisiae and candida as well as aspergillus niger, rhizopus oryzae and mucor racemosus, compounding, inoculating a wet wheat material, and fermenting to obtain a finished product. Sequentially carrying out three-stage variable-temperature fermentation of a temperature programming stage, a high-temperature top fire stage and a bacterium supplementing fermentation stage in a closed container, and finally, carrying out low-temperature drying, so as to obtain the finished product yeast. According to the method disclosed by the invention, through specific bacterial system combination and a whole-process controllable fermentation process, accurate regulation and control on a microbial metabolism process are realized, so that key indexes such as saccharifying power, esterifying power and acid protease activity of finished koji are stable and accord with industrial standards. The method is clear in process parameters and good in reproducibility, effectively overcomes regional dependence of traditional starter propagation, and provides reliable technical support for standardized and large-scale production of Luzhou-flavor liquor.

The present invention relates to a fermentation process utilizing seaweed biomass co-inoculated with a yeast strain and a carbohydrate source, wherein the fermentation process is performed in the presence of one or more Bacillus strains to increase the production of vitamin K2. In one embodiment, the seaweed is Kappaphycus alvarezii, the yeast is Saccharomyces cerevisiae, Candida utilis, or the mixture thereof, and the one or more Bacillus strains is Bacillus altitudinis, Bacillus pumilus, and / or Bacillus subtilis.

The invention discloses a prune and hawthorn fermented beverage and a preparation method thereof. The prune and the hawthorn are used as preparation raw materials, the beverage is prepared through probiotic fermentation, and the beverage has the advantages of being good in taste, rich in nutrient substance, high in oxidation resistance and the like. The lactobacillus delbrueckii MT772183 and the lactobacillus pentosus P-1 are combined for fermentation, so that the content of total flavonoids in the fermented beverage is increased, and the candida kristii XBL-1 and the hansenula polymorpha MY-00 are combined for fermentation, so that the content of total phenols in the fermented beverage is increased.