29 results about "[Candida] apicola" patented technology

This invention relates to a protein feed prepared from biorefined baijiu lees and its preparation method, belonging to the fields of bioengineering and feed resource development technology. It includes the following steps: (1) subjecting the baijiu lees to acid pretreatment and dual-enzyme synergistic hydrolysis sequentially to obtain a hydrolysate containing fermentable sugars; (2) adding Candida utilis culture and urea for carbon-nitrogen synergistic liquid fermentation to obtain a Candida utilis viable count ≥4.0 × 10⁻⁶. 8 Fermentation broth with CFU / mL; drying is then performed. This invention pretreats baijiu (Chinese liquor) lees under high pressure with dilute acid, followed by synergistic hydrolysis with two enzymes, and then utilizes *Candida utilis* for carbon-nitrogen synergistic liquid fermentation, achieving efficient utilization of glucose and xylose, with a cell protein yield ≥0.70 g / L / h. This invention realizes the high-value utilization of all components of baijiu lees, providing a process route with industrial-scale production potential for the biorefining of baijiu lees to prepare protein feed.

This invention belongs to the field of alcoholic beverage processing technology, specifically relating to a high-purity ginkgo wine and its preparation method. The preparation method comprises the following steps: ginkgo slurry undergoes preliminary enzymatic hydrolysis with neutral protease and amylase, followed by secondary enzymatic hydrolysis with pectinase and sodium bisulfite; brewing yeast fermentation broth is added for pre-fermentation; glutamine transaminase, *Saccharomyces cerevisiae* fermentation broth, and *Candida valerate* fermentation broth are added for post-fermentation; and finally, distillation and filtration are performed to obtain the ginkgo wine. This invention achieves complete decomposition and fermentation of ginkgo kernels through a two-step enzymatic hydrolysis and two-step fermentation process. The preparation method has advantages such as simple process and low cost, which is beneficial for industrial production; the prepared ginkgo wine has advantages such as clarity, strong aroma, high alcohol content, and low acidity.

The present disclosure provides a method for producing chemicals using halotolerant yeast. The method includes culturing halotolerant yeast cells in a medium comprising a solution of salts at a concentration of at least 0.5 times the salinity of seawater, and allowing the halotolerant yeast cells to produce one or more chemicals. The medium may include a carbon source, nitrogen source and / or phosphate source, trace elements, and additives. The halotolerant yeast cells may be selected from, e.g., Debaryomyces subglobosus, Candida taylori, Pichia anomaly Millerozyma farinosa, Blastobotrys adeninivorans, Scheffersomyces stipitis. or combinations thereof. The method enables chemical production in high-salt media, reducing freshwater usage in fermentation processes.

The invention relates to the technical field of microbial fermentation, in particular to a preparation method and application of a digestible biological protein fermentation material. According to the fermentation method, candida utilis serves as a fermentation strain, industrial and agricultural waste glucose mother liquor and corn steep liquor serve as substrates, high-yield mycoprotein is obtained under the relative low-temperature fermentation condition of 20-25 DEG C, and the obtained fermentation liquor is subjected to single enzymolysis, so that a fermentation material easier to digest is obtained. When weaned piglets are fed with the obtained fermented material, the diarrhea rate of the piglets can be effectively reduced.

The present application relates to high protein fermentation technical field, specifically disclose a kind of preparation method for fermenting production of yeast protein based on alcohol waste liquor, to solve the technical problems such as low utilization rate of alcohol waste liquor resource, feed soybean meal supply is nervous and high cost.The method includes the following steps: first, the alcohol waste liquor produced by grain deep processing is concentrated to dry basis content ≥30%, the composite carbon source carrier is crushed, urea and molasses are added to adjust the carbon-nitrogen ratio to (20~25):1, access to the complex fermentation strain containing Candida utilis, solid fermentation is carried out, and the finished product yeast protein is obtained.The present application uses solid fermentation mode, matches composite carbon source carrier to optimize nutrient ratio, step by step controls key process parameters, and the finished product yeast protein is ≥24%, true protein is ≥22%, and nitrogen conversion rate is ≥85%, which can replace soybean meal as feed additive.The method realizes alcohol waste liquor resource utilization, pollution is small, reduces feeding cost, has environmental protection and economic value, and is suitable for industrialization popularization and application.

The invention provides a method for preparing ruminant biological feed through fermentation of sodium humate and probiotics, and relates to the field of solid feed fermention.The method comprises the steps that S1, candida utilis powder, lactobacillus plantarum liquid, enterococcus faecium liquid and beet molasses are stirred and mixed, and molasses bacterial liquid is obtained; s2, mixing the molasses bacterial liquid with the treated sodium humate and gelatinized starch urea according to a preparation ratio, and uniformly stirring; and S3, loading into an anaerobic sealed fermentation bag, and naturally fermenting in a greenhouse at the temperature of 25-35 DEG C for 30-48 hours. The biological feed which is rich in high-activity probiotics, high in protein and easy to digest and absorb by animals is prepared from byproducts such as sodium humate, molasses and the like by utilizing biological engineering, and can replace antibiotics in the feed.

The application provides a vinegar residue-based single-cell protein feed and a preparation method and application thereof, and belongs to the technical field of feed preparation.The application uses vinegar residue as raw material, adopts solid-state fermentation of Aspergillus, Candida utilis and Saccharomyces cerevisiae in combination with composite enzymes, utilizes the synergistic effect among the strains and the enzymolysis effect of the exogenous enzymes, efficiently degrades the fibrous substances in the vinegar residue, and converts the fibrous substances into microbial proteins, so that the crude protein content of the prepared vinegar residue-based single-cell protein feed is increased from 5% to 8% of the raw material to 20% to 25%, and the total fibrous substance degradation rate is greater than or equal to 60%. The vinegar residue-based single-cell protein feed produced by the application has good feeding value, can effectively replace part of the protein raw materials such as soybean meal, improves the production performance of beef cattle, reduces the breeding cost, realizes high-value utilization of vinegar residue resources, and has good popularization and application prospect.

The invention discloses a formula of a fermented feed for enhancing animal immune function, and relates to the technical field of fermented feeds. The formula comprises the following components in parts by mass: a basic raw material, an immune enhancement extract, a functional additive, 0.3-0.6 part of a composite microbial agent and 2-4 parts of composite mineral substances. Wherein the ganoderma lucidum extract and the folium cortex eucommiae extract activate immune cells through the synergistic effect of polysaccharide and chlorogenic acid; the complex microbial inoculant is composed of lactobacillus acidophilus, bacillus licheniformis and candida utilis according to a ratio of (5-6): (3-4): 2, efficiently degrades antinutritional factors and generates immunocompetence metabolites. The preparation process comprises the following steps: supercritical extraction of plant active components, mixing and humidifying of base materials, segmented stirring and fermentation after microbial inoculum activation, and then addition of functional components and drying. Through synergistic combination of the raw materials and process optimization, the animal immunity is remarkably improved, and the feed additive has the advantages of low cost and multiple immune enhancement mechanisms.

The invention relates to the field of livestock and poultry breeding, and discloses a laying hen feed additive based on fermented broussonetia papyrifera and application of the laying hen feed additive. The additive is prepared by taking paper mulberry leaves as a main material, matching with rice husks, wheat bran and other auxiliary materials, inoculating a microecological preparation containing lactobacillus plantarum, candida utilis and bacillus subtilis, and performing anaerobic fermentation. 4% of the additive is used for daily ration of the aged laying hens, so that the laying rate can be remarkably increased, the dirty egg rate can be reduced, and the egg weight, the eggshell strength and the yolk color can be improved; the mechanism is to improve the form of the intestinal tract, enhance the expression of tight junction protein, regulate immune balance, optimize the structure of cecum flora and enrich beneficial microorganisms; the broussonetia papyrifera leaf feed additive realizes efficient utilization of broussonetia papyrifera leaf resources, provides a safe and effective functional additive for prolonging the laying period of laying hens and improving the breeding benefits, and has a good application prospect.

The invention discloses a preparation method and application of a rice fermentation product, and the preparation method comprises the following steps: S1, mixing rice, cold water and glucose according to a weight ratio of 1: (15-25): (0.01-0.03), soaking for 8 hours, and performing autoclaved sterilization at 100-121 DEG C for 10-20 minutes; s2, a composite yeast seed solution is inoculated into the sterilized rice mixed solution, the composite yeast seed solution is composed of candida utilis and Michelia yeast according to the weight ratio of (1-2): 1, and the weight ratio of rice to the composite yeast seed solution is (20-30): 1; s3, fermenting for 24-72 hours under the conditions of non-sealing, constant temperature of 26-30 DEG C and oscillation speed of 150 rpm / min, and controlling the pH value to be 4.5-6.0; s4, performing high-pressure microjet homogenization on the fermentation product at the rotating speed of 6-10rpm for 10-20 minutes; and S5, centrifuging the homogenized liquid for 30 minutes at 4000 rpm, collecting supernate, sterilizing for 20 minutes at 120 DEG C, filtering by using a microporous membrane of 0.45 mu m, and adding p-hydroxyacetophenone which accounts for 0.3-0.5% of the total weight of the filtrate and 1, 2-hexanediol which accounts for 0.5-1% of the total weight of the filtrate.

The present application relates to a protein feed containing a composite fermentation composition, and belongs to the technical field of microbial fermentation and feed.The protein feed comprises fermentation strains and fermentation substrates; the fermentation strains comprise Rhodopseudomonas palustris with a preservation number of CGMCC NO.17021 and Candida glabrata with a preservation number of CCTCC NO:M2015664; and the fermentation substrates comprise cottonseed meal powder, rapeseed meal powder, distiller's grains powder and citrus dreg powder.The protein feed containing the composite fermentation composition has a high feed utilization rate and can improve the immunity of the animals fed with the protein feed.

The invention discloses a method for improving the quality of sugarcane tip silage through lactic acid bacteria-saccharomycetes synergistic fermentation. The method comprises the following steps: (1) preparing silage raw materials; (2) preparing bacterial suspension of lactic acid bacteria and saccharomycetes; and (3) preparing and storing the silage. According to the method, based on the high-sugar raw material characteristic of sugar cane tips, lactic acid bacteria and saccharomycetes are added to synergistically regulate and control the quality of the sugar cane tip silage, and lactic acid bacteria (lactobacillus or lactococcus and leuconostoc) and saccharomycetes (candida boidinii and pichia kudriavzevii) are used for synergistically anaerobic fermentation in the silage process, so that the quality of the sugar cane tip silage is improved. The substrate sugar is decomposed to generate a large amount of lactic acid, and the pH value of the feed is effectively reduced, so that the growth of harmful microorganisms is inhibited, the generation of ethanol is reduced, more nutritional ingredients are retained, and the quality of the sugar cane tip silage is further improved.

The invention relates to a fermented Chinese pulsatilla root preparation for improving the yield of laying hens in humid and hot weather, relieving stress and improving the antioxidant function. Specifically, the invention provides application of the traditional Chinese medicine fermentation composition, and the traditional Chinese medicine fermentation composition is applied to one or more of the following groups: the traditional Chinese medicine fermentation composition is used for improving the feed conversion rate of poultry; the morbidity and mortality of poultry are reduced; the composition is used for reducing the hormone level related to high-temperature and high-humidity stress of poultry; the preparation method of the fermented Chinese pulsatilla root composition comprises the following steps: carrying out expanding culture on bacillus subtilis, lactobacillus plantarum and candida utilis to obtain compound probiotic liquid, and fermenting Chinese pulsatilla root powder by using the compound probiotic liquid to obtain the fermented composition. The feed has the advantages of solving the prominent problems of reduced immunity, high incidence of pathogenic bacteria infection, reduced production performance and the like caused by environmental stress in laying hen breeding in southern hot and humid areas, so as to improve the economic value of poultry production in summer.

The invention discloses a preparation method of a honey, royal jelly and propolis fermentation composition, the fermentation composition and application. The preparation method provided by the invention comprises the following steps: adding a first-stage substrate into a basic fermentation culture medium, inoculating candida albicans and kluyveromyces marxianus, and performing first-stage fermentation to obtain first fermentation liquor; supplementing a second-stage substrate into the first fermentation liquor, and carrying out second-stage fermentation to obtain second fermentation liquor; and performing cell disruption and post-treatment on the second fermentation liquid to obtain the honey, royal jelly and propolis fermentation composition. The candida albicans and the kluyveromyces marxianus are adopted to conduct two-stage synergistic fermentation on the three substrates of the honey, the royal jelly and the propolis, cell disruption is conducted after synergistic fermentation, yeast lysate is released, and the prepared fermentation composition is clear and transparent in appearance and good in stability, has the rich rose fragrance, and is suitable for being used as a raw material. And the capacity of inhibiting inflammatory factors and promoting collagen synthesis is remarkable.

The invention discloses a composition rich in natural B vitamins as well as a preparation method and application thereof, and belongs to the field of sports nourishment raw materials. The composition is prepared by fermenting raw materials including glucose, peptone, yeast extract, magnesium sulfate and the like by adopting mixed strains including saccharomyces cerevisiae ATCC26603, candida utilis ATCC9950 and rhodotorula glutinis ATCC32765; and treating by combining an ultrahigh pressure synergistic enzyme method process to obtain yeast extract powder which is rich in natural B vitamins and is relatively high in yeast protein yield. The yeast extract prepared by the invention has excellent muscle injury protection and repair effects, and the sports nourishment prepared by compounding the yeast extract with an embedding medium and an antioxidant can reduce muscle injury after sports, promote muscle development and meet core nutritional requirements of sports crowds.

The invention discloses a Lu-type Daqu liquor yeast medicine and a preparation method thereof, and belongs to the technical field of liquor brewing. The preparation method comprises the following steps: respectively preparing mixed seed solutions from lactobacillus, bacillus aceticus and bacillus subtilis, saccharomyces cerevisiae and candida as well as aspergillus niger, rhizopus oryzae and mucor racemosus, compounding, inoculating a wet wheat material, and fermenting to obtain a finished product. Sequentially carrying out three-stage variable-temperature fermentation of a temperature programming stage, a high-temperature top fire stage and a bacterium supplementing fermentation stage in a closed container, and finally, carrying out low-temperature drying, so as to obtain the finished product yeast. According to the method disclosed by the invention, through specific bacterial system combination and a whole-process controllable fermentation process, accurate regulation and control on a microbial metabolism process are realized, so that key indexes such as saccharifying power, esterifying power and acid protease activity of finished koji are stable and accord with industrial standards. The method is clear in process parameters and good in reproducibility, effectively overcomes regional dependence of traditional starter propagation, and provides reliable technical support for standardized and large-scale production of Luzhou-flavor liquor.

The invention discloses a prune and hawthorn fermented beverage and a preparation method thereof. The prune and the hawthorn are used as preparation raw materials, the beverage is prepared through probiotic fermentation, and the beverage has the advantages of being good in taste, rich in nutrient substance, high in oxidation resistance and the like. The lactobacillus delbrueckii MT772183 and the lactobacillus pentosus P-1 are combined for fermentation, so that the content of total flavonoids in the fermented beverage is increased, and the candida kristii XBL-1 and the hansenula polymorpha MY-00 are combined for fermentation, so that the content of total phenols in the fermented beverage is increased.

The invention discloses a preparation method of a traditional Chinese medicine compound enzyme for regulating qi and blood, and relates to the crossing field of biological fermentation technology and traditional Chinese medicine application, in particular to the preparation method of the traditional Chinese medicine compound enzyme for regulating qi and blood. The preparation method comprises the following steps: mixing the raw materials in proportion, performing vacuum freeze drying to obtain freeze-dried composite stem and leaf powder, mixing the freeze-dried composite stem and leaf powder with red date particles, adding water for enzymolysis, performing solid-liquid separation, taking residues, inoculating a mixed bacterium solution of candida utilis and lactobacillus plantarum, and performing discontinuous oxygen supply solid-state fermentation and aging to obtain a finished product. And a carbon source is derived from natural sugar and enzymolysis products of the red dates. The water content of the obtained enzyme is about 30-40%, the pH value is 3.8-4.2, and the enzyme has unique fermentation flavor and can be used for regulating qi and blood.

The present application relates to a kind of alternative fish meal complex enzymatic protein composition and its preparation method, belong to aquatic feed technical field.The composition includes protein composition, charcoal load phagostimulant granule and functional adjuvant, wherein protein composition is obtained by complex enzymolysis, solid-liquid separation after the enzymolysis substrate of corn gluten meal, pre-enzymolysis black soldier fly powder and chlorella powder composition is mixed with bran, inoculated bacillus subtilis and Candida utilis is made by mixed bacteria solid state fermentation;Charcoal load phagostimulant granule is made by loading on charcoal after the liquid part after solid-liquid separation is mixed with yeast extract.

The invention belongs to the technical field of animal husbandry feeds, and particularly relates to a biological fermentation feed and a complete feed used in the growth stage of Changrong binary pigs. The biological fermentation feed is prepared from fermented distiller's grains and fermented rapeseed dregs, and the mass ratio of the fermented distiller's grains to the fermented rapeseed dregs is 1: (1.5-5); the feeding object is a hybrid pig (Changbai pig and Rongchang pig) of a Changbai pig and a Rongchang pig. The fermented white spirit vinasse is prepared by fermenting cellulase, pectinase, candida tropicalis, bacillus subtilis and lactobacillus plantarum; the fermented rapeseed meal is prepared by fermenting lactobacillus plantarum, candida tropicalis, acid protease, cellulase, beta-glucanase and xylanase. The complete feed prepared from the biological fermentation feed provided by the invention is used for feeding the Changrong binary pigs in the growth stage, so that the feed cost of the Changrong binary pigs is reduced, the economic benefits of the Changrong binary pigs are improved, and meanwhile, the disease resistance of the Changrong binary pigs is enhanced.

The invention discloses a straw fermentation method, and belongs to the technical field of straw feed application. The straw fermentation method comprises the following steps: selecting straw and fresh forage grass, mixing the selected straw and fresh forage grass in proportion to obtain a mixed raw material, adding a fermentation inoculant, wrapping with a breathing bag, and fermenting in a shade place in a dark place to obtain a mixed storage feed; wherein the weight ratio of the straw to the fresh forage grass is 1: (2-3); the fermentation inoculant comprises the following components in parts by weight: 0.4-0.6 part of urea; 1.5 to 2.0 parts of molasses; 5.0 to 10 parts of aspergillus niger; 5.0 to 10 parts of bacillus subtilis; 10 to 15 parts of cellulase; 10 to 15 parts of xylanase; 20 to 30 parts of candida utilis; 20 to 30 parts of beta-glucanase; 30 to 50 parts of lactobacillus plantarum; the addition amount of the fermentation inoculant is 0.1-0.2% of the total weight of the mixed raw materials, and the straw and the fresh forage grass are mixed with the specific fermentation inoculant for synergistic fermentation, so that the fermentation quality and the nutritional level of straw feed are comprehensively improved. The problems that existing straw is low in nutritional value and poor in digestibility and palatability, and consequently the feed conversion rate is low are solved.

This application discloses a method for preparing a fermented composition of honey, royal jelly, and propolis, the fermented composition itself, and its applications. The preparation method provided by this application includes: adding a first-stage substrate to a basic fermentation medium, inoculating with *Candida apis* and *Kluyveromyces martensii*, and carrying out a first-stage fermentation to obtain a first fermentation broth; adding a second-stage substrate to the first fermentation broth and carrying out a second-stage fermentation to obtain a second fermentation broth; and subjecting the second fermentation broth to cell disruption and post-treatment to obtain the fermented composition of honey, royal jelly, and propolis. This application uses *Candida apis* and *Kluyveromyces martensii* to carry out a two-stage synergistic fermentation of honey, royal jelly, and propolis as a triple substrate. After synergistic fermentation, cell disruption is performed to release yeast lysates. The resulting fermented composition is clear and transparent in appearance, has good stability, a rich rose aroma, and significantly inhibits inflammatory factors and promotes collagen synthesis.

The disclosed subject matter relates generally to a method for modifying oil, and specifically to a process for increasing the concentration of polyunsaturated fatty acid in an oil composition. The process comprises a) hydrolysis of the glyceride by Thermomyces lanuginosus lipase, b) separation of the free saturated fatty acids from the glycerides and c) enzymatic esterification of the hydrolyzed glyceride by Candida antarctica lipase B with a polyunsaturated fatty acid.

The invention discloses a method for preparing a biological feed through mixed fermentation of fen-flavor vinasse, and belongs to the field of feed processing. Aiming at the problems that fresh fen-flavor distillers' grains are high in water content, easy to decay, low in crude protein and poor in utilization rate, the method comprises the following steps: adjusting the water content of the distillers' grains to 65-75% through pretreatment, adding a rape seed cake-bran composite carbon and nitrogen source, inoculating saccharomyces cerevisiae, rhizopus oryzae, candida utilis, bacillus subtilis and lactobacillus plantarum in stages, and fermenting to obtain the fresh fen-flavor distillers' grains. Carrying out anaerobic fermentation at 30-35 DEG C for 5-7 days, and drying at 60-65 DEG C until the water content is 1t; the product is prepared from 13% of raw materials. The five bacteria cooperate to significantly improve crude protein (20% + higher than that before fermentation, and dry substance crude protein is greater than or equal to 28%), inhibit infectious microbes, prolong the storage period to 6 months or above, and realize high-value utilization of vinasse, and the process is simple and low in cost.

The invention provides a mutton sheep feed additive for increasing weight and improving meat quality as well as a preparation method and application thereof, and belongs to the technical field of feed additives. The mutton sheep feed additive comprises Chinese herbal medicines such as dried orange peel, scorched hawthorn fruit, fried malt and radish seed, and lactobacillus plantarum, bacillus licheniformis, candida utilis, neutral protease, xylanase and lipase, and can be added into basic daily feed of mutton sheep for reinforced conditioning feeding. Through the synergistic effect of the Chinese herbal medicine, the beneficial bacteria and the active enzyme, the problems of rumen acidification, digestion disorder and the like caused by high-concentrate fattening are solved, the meat indexes such as muscle tenderness and water-holding capacity are improved while the weight gain rate of mutton sheep is increased, the cost waste of whole-process feeding is avoided, and the feed is suitable for large-scale mutton sheep breeding application.

The invention belongs to the technical field of animal feeds, and particularly relates to a beef cattle breeding straw feed formula, which takes sugarcane tail tips, corn straws, mulberry twigs, high-proportion cassava residues and other agricultural and forestry byproducts as base materials, and is matched with molasses, a composite biological leavening agent (containing trichoderma reesei, candida utilis, lactobacillus plantarum and laccase) and a nutrition additive. Through a synergistic process of mulberry branch steam explosion modification, pre-acidolysis stack retting and staged oxygen-controlled fermentation, resource utilization of agricultural and forestry wastes is realized, crude fibers are obviously degraded, cyanoglycoside residues are reduced, and the content of crude proteins is increased. The feed is good in palatability, stable in storage, capable of improving the daily gain of beef cattle and reducing the feed conversion rate in breeding, low in cost and controllable in process, has both environmental protection and economic benefits, and is suitable for large-scale application.

The present invent ion concerns a method for preparing a fatty amidoalkyldialkylamine by reacting a fatty acid with a dialkylaminoalkylamine, using a molar ratio of said dialkylaminoalkylamine to said fatty acid of more than 1 and up to 1.5, in the presence of Candida antarctica lipase as catalyst.

The application relates to the technical field of biological fertilizers, and particularly discloses a fruit and vegetable coloring and sweetening biological fertilizer containing fish bone powder extract and a preparation method thereof. The fruit and vegetable coloring and sweetening biological fertilizer containing fish bone powder extract comprises, in terms of weight parts, 25-35 parts of organic fermentation material, 40-60 parts of inorganic fermentation material, 8-12 parts of compound microbial bacteria, 15-25 parts of fish bone powder extract, 5-10 parts of chitosan quaternary ammonium salt, 4-6 parts of a functional supplement and 1-2 parts of a nutrient supplement; the compound microbial bacteria comprises Bacillus subtilis, Paenibacillus mucilaginosus, Candida utilis and Rhodopseudomonas palustris. The fruit and vegetable coloring and sweetening biological fertilizer containing fish bone powder extract can significantly improve the formation and precipitation of fruit and vegetable color and the sweet taste of the fruit and vegetable.

The invention discloses a method for preparing sophorolipid with the mass ratio of lactone type sophorolipid not lower than 50% from kitchen waste grease, and belongs to the technical field of sophorolipid preparation. According to the method, candida is used as a production strain, glucose is used as a fermentation substrate, dissolved oxygen and temperature are controlled to start fermentation, glucose and kitchen waste grease are added in the fermentation process until fermentation is finished, and the sophorolipid with the mass ratio of lactone type sophorolipid not lower than 50% is obtained through post-treatment of fermentation liquor. By optimizing fermentation strategies such as specific inducers and pH control, the target lactone sophorolipid is directly, efficiently and highly selectively synthesized by microorganisms in the fermentation process, synthesis of acid type products is greatly reduced or even eliminated, the product specificity is improved, and the mass content of the lactone sophorolipid accounts for 50-90% of the total sophorolipid.