128 results about "[Candida] apicola" patented technology

The invention relates to micro-ecological Chinese medicine preparation for livestock and poultry by adoption of multi-strain fermentation production and a fermentation method. The compositions of traditional Chinese medicines in a culture medium of the fermentation preparation include: radix astragali, radix codonopsitis, Atractylodes macrocephala, Poria cocos, liquorice, medicated leaven, hawkthorn, angelica, Chinese rhubarb, Scutellaria baicalensis, radix isatidis, cordate houttuynia, sicklesenna seeds, Schisandra chinensis, Gynostemma pentaphylla, phellodendron, dried orange peel, radix bupleuri, curcuma, honeysuckle, Chinese gall, purslane and Quisqualis indica. The fermentation method is as follows: a multi-strain solid state fermentation strain is adopted for fermentation; bacillus subtilis, bacillus natto, bacillus licheniformis, beer yeast, Candida wtilis, Aspergillus niger, lactobacillus plantarum, Lactobacillus acidophilus and bifidobacteria are purified and subjected to anaerobic fermentation under the conditions of a rotating speed of 180 revolutions per minute at a temperature is 32 DEG C for 48 to 72 hours till the pH value reaches 4.0. The micro-ecological Chinese medicine preparation has the advantages that the micro-ecological Chinese medicine preparation uses probiotics to ferment different Chinese medicine compositions, and is micro-ecological Chinese medicine preparation which completely replaces antibiotics and has the advantages of no residual medicine, disease prevention, growth promotion and low cost.

The invention relates to an early weaning meat sheep fattening compound biological feed additive, which belongs to the technical field of animals feeding by feed. The feed additive comprises prebiotics consisting of candida utilis, bacillus subtilis and a lactobacillus plantarum microbial agent, non-starch polysaccharide enzyme containing cellulase, xylanase, beta-dextranase, beta-mannase and pectinase and a yeast culture formed by using saccharomyces cerevisiae to completely ferment bean pulp. The additive is specially used for fattening period ration of the early weaning(the weaning lunar age is less than or equal to 2 ages of the moon) meat sheep, and the adding percentage for the full-mixing ration of the fast-fattening meat sheep in 3 to 5 ages of the moon is 1 percent. The additive can provide functional nutrients such as digestive enzyme, B group vitamins and growth promoting factors, and the like which are inadequately produced and insufficient for the early weaning baby sheep and simultaneously contains probiotics for adjusting the micro-ecological environment in intestinal canals, thereby improving the immunity and the healthy level of the baby meat sheep; and after weaning, the daily gaining in weight is more than 10 percent in the prior period and later period of fattening, and the economic benefits are obviously improved.

The present invention discloses a special penaeus orientalis functional biological feed in the thickness marking period and the functional biological feed consists of the following components: import fish meal, shrimp meal, fermented peanut meal, fermented soybean meal, yeast lysates, cuttlefish powder, high gluten flour, calcium dihydrogen phosphate, soybean lecithin, fish oil, vitamin-mineral premixes, probiotics and functional additives; wherein the probiotics consist of Bacillus subtilis subsp., Candida utilis yeast and Bifidobacterium longum; the functional additives are made of natural astaxanthin, linolenic acid, fermented chlorella and fermented spirulina. The functional biological feed aims at the physical characteristics of high density, high nutrition and fast growth of penaeus orientalis seedlings in the thickness marking period, uses high-quality animal protein, contains high levels of unsaturated fatty acids and bio-fermentation raw materials, is rich in lecithin, has a reasonable balance ratio of calcium and phosphorus, and fortified vitamin nutrition, adds immunoenhancers, living beneficial bacteria and digestive enzymes, promotes the rapid growth, enhances disease resistance and improves survival rate of penaeus orientalis seedlings which have strong body constitution and tidy specifications, and lay a solid foundation for subsequent successful breeding.

The invention relates to a preparation method of a high-efficiency compound microorganism soybean meal leaven, belonging to the technical field of biological enzymolysis and fermentation. The preparation method comprises the following steps of: respectively carrying out optimal screening on lactobacillus acidophilus, lactobacillus lactis, saccharomyces cerevisiae, Candida utilis, bacillus subtilis and bacillus licheniformis; mixing and culturing by shaking a bottle; culturing by using a 10L fermentation tank; manufacturing a microorganism compound protective agent; spray drying in low temperature; preparing the soybean meal leaven and the like. The high-efficiency compound microorganism soybean meal leaven can efficiently inhibit the growth of mixed bacterium and eliminate or remarkably lower anti-nutritional factors and flatulent factors in soybean meal, enhance the protein utilization ratio and strengthen the immunity and has the excellent characteristics of acid resistance, salt resistance, temperature resistance and bile resistance. The invention has the advantages of simple preparation method, low cost, stable and reliable produced product quality and wide application range.

The invention discloses a method of producing a protein feed by a liquid-solid two-step fermentation method. The method comprises the following steps: performing liquid culture on corynebacterium glutamicum, candida utilis, bacillus subtilis, lactobacillus plantarum and aspergillus oryzae so as to obtain corynebacterium glutamicum fermentation liquor, candida utilis fermentation liquor, bacillus subtilis fermentation liquor, lactobacillus plantarum fermentation liquor and aspergillus oryzae fermentation liquor; mixing the corynebacterium glutamicum fermentation liquor, the candida utilis fermentation liquor, the bacillus subtilis fermentation liquor, the lactobacillus plantarum fermentation liquor and the aspergillus oryzae fermentation liquor so as to obtain mixed bacterium liquor; loading raw materials of soybean meal, cluster bean meal, cottonseed meal and the like into an explosion vat for performing steam explosion; after steam explosion, mixing materials, the mixed bacterium liquor and molasses in proportion, and adding an enzyme preparation so as to obtain mixed materials; conveying the mixed materials into a special modularized solid-state biological raw material continuous fermentation device for enzymolysis and fermentation, and drying the fermented materials so as to obtain the protein feed. According to the method disclosed by the invention, the modularized automatic fermentation device is adopted, the materials after explosion, the fermented bacterium liquor and the enzyme preparation are mixed, then the mixed materials are loaded into the automatic fermentation device for enzymolysis and biological fermentation, so that requirements for automatic temperature control, automatic dampness control and automatic ventilation control are met, the bioconversion rate is high, the fermentation period is short, the consumption is low, the cost is low, and mechanized and large-scale production requirements can be met.

A microbial ethyl esther sophorolipid derivative with no acetylated groups produced by Candida species, for treating and preventing sepsis/septic shock. The method of producing sophorolipids is through microbial resting cells of Candida bombicola. The sophorolipids obtained from resting state cultures are isolated as a complex mixture of compounds and then decanted as a dense oil from the culture broth, subsequently washed to remove free fatty acids. Secondary chemical transformation via base catalyzed hydrolysis is used to reduce the 8 possible structural sophorolipid species to a single moiety, the 17-L-[(2′-O-b-D-glucopyranosyl-b-D-glucopyranosyl)-oxy]-cis-9-octadecenoate de-acetylated free acid. The compound acts primarily through decreasing inflammatory cytokines and eliciting other synergistic anti-inflammatory mechanisms by blocking TLR4-CD14 upstream of the inflammatory signaling cascade. The compound can be administered either intraperitoneally or intravenously at single or multiple doses of 5-30 mg/kg of weight in solvent media or in capped nanoparticles, preferably within 48 hours of sepsis inception.

The invention discloses a preparation method of fermented rice bran feed and an application thereof. The preparation method of the fermented rice bran feed disclosed by the invention comprises the following steps: adding a complex microbial inoculant and a complex enzyme preparation into a fermentation raw material taking rice bran as a main component to obtain a fermentation system, and fermenting the fermentation system to obtain a fermentation product which is the fermented rice bran feed, wherein the active ingredients of the complex microbial inoculant are bacillus subtilis, bacillus licheniformis, lactobacillus plantarum, lactobacillus casei, enterococcus faecalis, saccharomyces cerevisiae and candida utilis; the active ingredients of the complex enzyme preparation are cellulase, acid protease, amylase, beta-mannase, xylanase and beta-glucanase. The shelf life of the obtained fermented rice bran feed is prolonged, and the production performance of mammals and the yield and quality of produced milk can be further improved by utilizing the obtained rice bran fermented feed.

A novel method of very efficiently elevating the yield of oligosacchrides containing α-galactosyl, compared with the conventional methods, which comprises treating galactose or a galactose-containing material with a specific α-galactosidase and thus performing a dehydrocondensation reaction at a high substrate concentration. Anti-candida compositions originating in foods, having a high safety and excellent anti-candida effect and not being restricted in the supply, which contain as the active ingredient oligosacchrides obtained by treating galactose or a galactose-containing material with an α-galactosidase and thus performing a dehydrocondensation.

The invention provides a preparation method for feed. The preparation method comprises the following steps: (a) mixing crushed crop straws and livestock feathers to obtain a mixture; (b) carrying out steam explosion treatment on the mixture to obtain a steam explosion material; (c) mixing the steam explosion material obtained by the step (b) with bran and nutrition liquid to obtain a culture medium; sequentially inoculating filamentous fungi, candida utilis and lactic acid bacteria on the culture medium and fermenting; (d) drying the culture medium fermented in the step (c) to obtain the feed. According to the preparation method for the feed provided by the invention, the feed is high in production efficiency, abundant in nutrient substances and easy to digest by livestock.

The invention discloses a method for increasing content of ethyl caproate in Luzhou-flavor liquor. The method comprises the following steps: preparing a bacterial solution A, preparing a bacterial solution B, and preparing a composite functional bacterial solution C; preparing reinforced Daqu D; and preparation of pit mud microorganism enrichment liquid E and fermentation in a pool. According to the method, bacillus siamensis and candida utilis are selected for culture and fermentation, reinforced yeast is prepared, and the content of an ethyl caproate precursor substrate in the pit is increased through the synergistic effect of the reinforced yeast and the pit mud microorganism enrichment liquid, so that synthesis of ethyl caproate is promoted. When the method is used for brewing the Luzhou-flavor liquor, the ethyl caproate content of the raw liquor produced by the new cellar and the aging cellar is obviously improved, the Luzhou-flavor style of the liquor body is prominent, and the aroma is mellow.

The invention discloses a method for producing a feed through pretreating at a high temperature and fermenting at a low temperature. The method comprises the following steps: first utilizing amylases, celluloses, hemicellulase, bacillus coagulans, bacillus licheniformis, tropophilous bacilli, proteus hause, chelating cocci, lignocellulolytic microorganisms and the like as degrading factors, and degrading starch, celluloses and hemicelluloses in the materials into monosaccharides or polysaccharides at 40-70 DEG C; then inoculating the materials with candida utilis, monilia tropicalis, saccharomyces cerevisiae, lactobacilli and bacillus subtilis by a fermenting technology, and fermenting at 20-40 DEG C to make a high-protein feed with oligomerization functional sugar for cattle, sheep, pigs, chicken or other animals. The method can solve the insufficient protein problem of animal husbandry, enhance animal constitutions, relieve the pressure that people and livestock contest with crop and reduce pollution, and has significant economical and social benefits.

The invention discloses a complex fungicide for improving feed fiber degradation rate and positively regulating rumen fermentation propionic acid generation as well as application thereof, belonging to the technical field of livestock and poultry feed. The complex fungicide contains bacillus subtilis, candida utilis and enterococcus faecium. According to the complex fungicide provided by the invention, the fiber digestibility of rumen microbial feed can be obviously improved, the total production amount of total rumen microbial fermentation volatile fatty acids is increased by 21.1 percent, and the positive regulation amount of gluconeogenesis precursor propionic acid production amount is 10.5-11.3 percent. The preparation method is simple and high in operability and is suitable for feed processing in livestock and poultry industry, and the ruminant feed energy utilization efficiency can be obviously improved.

The invention discloses a method for preparing selenium-enriched yeasts by using Candida utilis, which relates to a novel method for preparing selenium-enriched yeasts. The method for preparing the selenium-enriched yeasts comprises the following steps: a, reviving Candida utilis with a collection number of CICC 1422 and preparing seeds; b, inoculating the seeds obtained by the step a onto a fermentation medium to ferment the seeds for 24 to 36 hours; c, adding selenium into fermentation liquor till the concentration of the selenium is 5 to 20mg/L, continuing fermentation for 24 to 48 hours, and collecting bacterium solution; and d, centrifuging the bacterium solution obtained by the step c, collecting thalli, drying and obtaining the selenium-enriched yeasts. In the method, the production cost, the biomass of the selenium-enriched yeasts, the selenium content in the selenium-enriched yeast can be taken into better consideration at the same time, selenium-enriched yeasts with high biomass and high selenium content can be obtained under a condition of small inorganic selenium consumption, and the method is suitable for industrial production.

The invention provides sweet potato dreg type fermented feed which is prepared through the fermentation technology of candida utilis and the like by using the abundant sweet potato dreg resources in areas where sweet potatoes are planted densely. According to the sweet potato dreg type fermented feed, the existing technology of fermented sweet potato dregs is improved, production parameters are made clear, production links are reduced, and the fermented sweet potato dregs with the higher feeding value are acquired.

The invention discloses a method for producing protein feed raw materials by carrying out enzymolysis and fermentation on white spirit vinasse. The method comprises the following steps of: culturing a candida tropicalis fermentation solution, a bacillus natto fermentation solution and a lactobacillus plantarum fermentation solution in liquid, and preparing into a mixed bacterium solution according to the ratio; filling the white spirit vinasse and CaO into an explosion cylinder according to the ratio; carrying out steam explosion under a certain condition; mixing exploded materials, the mixed bacterium solution and corn slurry at the ratio; meanwhile, adding an enzyme preparation to prepare a mixed material; and feeding the mixed material into a special modularized solid-state biological raw material continuous fermentation device to carry out enzymolysis and biological fermentation; and drying the fermented materials to obtain the protein feed raw materials. According to the method disclosed by the invention, the exploded materials, the fermented bacterium solution and the enzyme preparation are mixed and then are conveyed into the special modularized solid-state biological raw material continuous fermentation device to carry out the enzymolysis and biological fermentation, so that the requirements of automatic temperature control, humidity control and ventilation amount control are realized; the biological conversion rate is high, the fermentation period is short, the loss is less, the cost is low, and the mechanical and large-scale production can be realized.

The invention discloses a production method of high-protein feed containing arginine. The production method comprises the following steps: performing liquid fermentation with corynebacterium glutamicum, candida utilis, lactobacillus plantarum and bacillus natto; mixing fermented mash rich in metabolic products with mash rich in thalluses, then mixing the mixture with soybean meal and the like in proportion, and performing solid state fermentation; finally drying products, and packaging the dried products so as to obtain final products containing crude protein of which the content is higher than 50% and arginine of which the content is higher than 4.0%. The production method disclosed by the invention has the advantages that the technology is simple, mixed fermentation is directly performed on the fermented mash and solid materials, the microbial metabolism product namely the arginine is utilized, so that the time of the solid state fermentation is greatly shortened, and the probability of infectious microbes infection in the solid state fermentation process of raw materials is reduced; the energy consumption is low, and waste is not discharged in the fermentation process; according to feed raw materials prepared by the production method, the content of anti-nutritional factors in a plant meal group is reduced, the content of the arginine is higher than 4.0%, the additive amount of exogenous arginine can be reduced or replaced, and the cost of the feed is reduced.

The invention discloses a method for producing protein feed raw materials by carrying out enzymolysis and fermentation on soybean meal. The method comprises the following steps of: culturing a candida utilis fermentation solution, a bacillus subtilis fermentation solution and a lactobacillus acidophilus fermentation solution in liquid, and preparing into a mixed bacterium solution according to the ratio; filling the soybean meal into an explosion cylinder; carrying out steam explosion under a certain condition; mixing exploded materials, the mixed bacterium solution and molasses at the ratio; meanwhile, adding an enzyme preparation to prepare a mixed material; feeding the mixed material into a special modularized solid-state biological raw material continuous fermentation device to carry out enzymolysis and biological fermentation; and drying the fermented materials to obtain the protein feed raw materials. According to the method disclosed by the invention, the exploded materials, the fermented bacterium solution and the enzyme preparation are mixed and then are conveyed into the special modularized solid-state biological raw material continuous fermentation device to carry out the enzymolysis and biological fermentation, so that the requirements of automatic temperature control, humidity control and ventilation amount control are realized; the biological conversion rate is high, the fermentation period is short, the loss is less, the cost is low, and the mechanical and large-scale production can be realized.

The invention discloses a method of producing stalk ethanol by means of mixed fermentation of transgenetic yeast. The transgenetic yeast can respectively degrade cellulose and xylan of stalks to glucose and xylose and ferment glucose and xylose to ethanol without additionally providing cellulase and xylanase. Degradation of cellulose and xylan and fermentation are synchronously carried out. The method disclosed by the invention has the advantages that by means of transgenetic saccharomyces cerevisiae, transgenetic pichia stipitis and transgenetic candida shehatae, synchronous enzyme hydrolysis-fermentation of a stalk cellulose raw material is realized to produce ethanol without additionally adding cellulase and xylanase, thereby greatly lowering the production cost of stalk cellulose ethanol and promoting the progress of replacing petroleum fuel by the stalk cellulose ethanol.

The invention relates to a microbial inoculum combination applied to livestock and poultry manure fermentation, and a preparation method and use method thereof, and belongs to the field of composting.According to the invention, four mixed probiotics comprising lactobacillus buchneri, bacillus licheniformis BNQ-5, lactobacillus plantarum BNQ-32 and candida utilis are used to perform mixing culturein four enhancing culture solutions to obtain a No.I microbial inoculum, a No.II microbial inoculum, a No.III microbial inoculum and a No.IV microbial inoculums respectively special for chicken manure, pig manure, sheep manure and cow manure. In a preparation process, through selection of a novel protective agent and selection of an optimal bacterial liquid adsorption material, compost of the microbial inocula is quick in temperature rise, and the decomposition effect is good, so that the microbial inocula are not only suitable for warm weather in the south, but also suitable for compost fermentation in cold weather in the north. The invention is helpful for farmers to select more targeted fermentation microbial inocula, and rapid fermentation and decomposition of livestock manure are realized.

The invention discloses a production method of high protein feed rich in tryptophan. The production method includes: subjecting corynebacterium glutamicum, candida tropicalis, lactobacillus plantarum and bacillus natto to liquid fermentation, mixing fermented liquor rich in metabolites and living bacteria, mixing mixture with soybean meal and the like proportionally, performing solid fermentation, and drying and packaging a fermentation product to obtain the high protein feed higher than 50% in crude protein content and higher than 0.9% in tryptophan content. The production method has the advantages that the process is simple, the fermented liquor is directly mixed with solid material for fermentation, the metabolite, tryptophan, of microorganisms is made use, solid fermentation time is greatly shortened, chances of sundry bacteria contaminating in the solid fermentation process of raw materials are slimmed, energy consumption is low, no waste is discharged in the fermentation process, the feed produced has lowered content of anti-nutritional factors in plant meals, the content of the tryptophan is higher than 0.9%, less exogenous tryptophan is added or the tryptophan is replaced, and cost of the feed is reduced.

The invention discloses a method for removing aflatoxin from feed through fermentation and a preparation method of fermented feed. According to the method, candida utilis CGMCC 2.2066 is inoculated into feed to be processed for fermentation so as to achieve removal of the aflatoxin from the feed. According to the method, through high-efficiency degradation of the candida utilis CGMCC 2.2066 to the aflatoxin, high-efficiency effect for biologically removing the aflatoxin from the feed is achieved.

The invention provides an animal feed bean meal enriched in organic selenium and a preparation process thereof. In the invention, saccharomyces cerivisiae, candida utilis, lactobacillus acidophilus and bacillus licheniformis are taken as bacterial strains; the bean meal is added with a certain proportion of molasses, ammonium sulfate and sodium selenite as a culture media for fermentation; then drying is carried out to transform inorganic selenium into the organic selenium, which causes the finished product to contain abundant protein and the organic selenium; the components of the bean meal comprises 50-60% of crude protein, 7-10% of moisture, 0-0.002% of urease and 1-10ppm of the organic selenium. The feed manufactured by using the protein raw material does not need to be added with selenide additives and avoids the disadvantages caused by using the bean meal, and the bean meal has low cost and simple preparation method.