134 results about "[Candida] apicola" patented technology

The invention relates to micro-ecological Chinese medicine preparation for livestock and poultry by adoption of multi-strain fermentation production and a fermentation method. The compositions of traditional Chinese medicines in a culture medium of the fermentation preparation include: radix astragali, radix codonopsitis, Atractylodes macrocephala, Poria cocos, liquorice, medicated leaven, hawkthorn, angelica, Chinese rhubarb, Scutellaria baicalensis, radix isatidis, cordate houttuynia, sicklesenna seeds, Schisandra chinensis, Gynostemma pentaphylla, phellodendron, dried orange peel, radix bupleuri, curcuma, honeysuckle, Chinese gall, purslane and Quisqualis indica. The fermentation method is as follows: a multi-strain solid state fermentation strain is adopted for fermentation; bacillus subtilis, bacillus natto, bacillus licheniformis, beer yeast, Candida wtilis, Aspergillus niger, lactobacillus plantarum, Lactobacillus acidophilus and bifidobacteria are purified and subjected to anaerobic fermentation under the conditions of a rotating speed of 180 revolutions per minute at a temperature is 32 DEG C for 48 to 72 hours till the pH value reaches 4.0. The micro-ecological Chinese medicine preparation has the advantages that the micro-ecological Chinese medicine preparation uses probiotics to ferment different Chinese medicine compositions, and is micro-ecological Chinese medicine preparation which completely replaces antibiotics and has the advantages of no residual medicine, disease prevention, growth promotion and low cost.

The invention relates to an early weaning meat sheep fattening compound biological feed additive, which belongs to the technical field of animals feeding by feed. The feed additive comprises prebiotics consisting of candida utilis, bacillus subtilis and a lactobacillus plantarum microbial agent, non-starch polysaccharide enzyme containing cellulase, xylanase, beta-dextranase, beta-mannase and pectinase and a yeast culture formed by using saccharomyces cerevisiae to completely ferment bean pulp. The additive is specially used for fattening period ration of the early weaning(the weaning lunar age is less than or equal to 2 ages of the moon) meat sheep, and the adding percentage for the full-mixing ration of the fast-fattening meat sheep in 3 to 5 ages of the moon is 1 percent. The additive can provide functional nutrients such as digestive enzyme, B group vitamins and growth promoting factors, and the like which are inadequately produced and insufficient for the early weaning baby sheep and simultaneously contains probiotics for adjusting the micro-ecological environment in intestinal canals, thereby improving the immunity and the healthy level of the baby meat sheep; and after weaning, the daily gaining in weight is more than 10 percent in the prior period and later period of fattening, and the economic benefits are obviously improved.

The invention relates to a method for preparing selenium-rich low-cholesterol egg and the relevant product, belonging to health-care functional egg product production field, which comprises following steps: employing lactobacillus acidophilus and candida as bacteria, the culture medium is 0.5-2% yeast concrete and 0.5-2% glucose, inoculating bacteria in sodium selenite containing culture medium, culturing for 30-40hours, getting culturing solution containing selenium-rich probiotics, adding culturing solution in basic corn bean meal type ration , feeding chicken with it, and getting selenium-rich low-cholesterol egg products. The selenium content in egg products is 0.358-0.508 mg / kg (wet weight), cholesterol in egg yellow content is 11.37-12.17 mg / g (wet weight). The egg product is characterized by abundant nutrition, selenium supplement, low-cholesterol content, health-care function and effect of prevention of cardiovascular disease.

The present invention discloses a special penaeus orientalis functional biological feed in the thickness marking period and the functional biological feed consists of the following components: import fish meal, shrimp meal, fermented peanut meal, fermented soybean meal, yeast lysates, cuttlefish powder, high gluten flour, calcium dihydrogen phosphate, soybean lecithin, fish oil, vitamin-mineral premixes, probiotics and functional additives; wherein the probiotics consist of Bacillus subtilis subsp., Candida utilis yeast and Bifidobacterium longum; the functional additives are made of natural astaxanthin, linolenic acid, fermented chlorella and fermented spirulina. The functional biological feed aims at the physical characteristics of high density, high nutrition and fast growth of penaeus orientalis seedlings in the thickness marking period, uses high-quality animal protein, contains high levels of unsaturated fatty acids and bio-fermentation raw materials, is rich in lecithin, has a reasonable balance ratio of calcium and phosphorus, and fortified vitamin nutrition, adds immunoenhancers, living beneficial bacteria and digestive enzymes, promotes the rapid growth, enhances disease resistance and improves survival rate of penaeus orientalis seedlings which have strong body constitution and tidy specifications, and lay a solid foundation for subsequent successful breeding.

The invention relates to a preparation method of a high-efficiency compound microorganism soybean meal leaven, belonging to the technical field of biological enzymolysis and fermentation. The preparation method comprises the following steps of: respectively carrying out optimal screening on lactobacillus acidophilus, lactobacillus lactis, saccharomyces cerevisiae, Candida utilis, bacillus subtilis and bacillus licheniformis; mixing and culturing by shaking a bottle; culturing by using a 10L fermentation tank; manufacturing a microorganism compound protective agent; spray drying in low temperature; preparing the soybean meal leaven and the like. The high-efficiency compound microorganism soybean meal leaven can efficiently inhibit the growth of mixed bacterium and eliminate or remarkably lower anti-nutritional factors and flatulent factors in soybean meal, enhance the protein utilization ratio and strengthen the immunity and has the excellent characteristics of acid resistance, salt resistance, temperature resistance and bile resistance. The invention has the advantages of simple preparation method, low cost, stable and reliable produced product quality and wide application range.

The invention discloses a method of producing a protein feed by a liquid-solid two-step fermentation method. The method comprises the following steps: performing liquid culture on corynebacterium glutamicum, candida utilis, bacillus subtilis, lactobacillus plantarum and aspergillus oryzae so as to obtain corynebacterium glutamicum fermentation liquor, candida utilis fermentation liquor, bacillus subtilis fermentation liquor, lactobacillus plantarum fermentation liquor and aspergillus oryzae fermentation liquor; mixing the corynebacterium glutamicum fermentation liquor, the candida utilis fermentation liquor, the bacillus subtilis fermentation liquor, the lactobacillus plantarum fermentation liquor and the aspergillus oryzae fermentation liquor so as to obtain mixed bacterium liquor; loading raw materials of soybean meal, cluster bean meal, cottonseed meal and the like into an explosion vat for performing steam explosion; after steam explosion, mixing materials, the mixed bacterium liquor and molasses in proportion, and adding an enzyme preparation so as to obtain mixed materials; conveying the mixed materials into a special modularized solid-state biological raw material continuous fermentation device for enzymolysis and fermentation, and drying the fermented materials so as to obtain the protein feed. According to the method disclosed by the invention, the modularized automatic fermentation device is adopted, the materials after explosion, the fermented bacterium liquor and the enzyme preparation are mixed, then the mixed materials are loaded into the automatic fermentation device for enzymolysis and biological fermentation, so that requirements for automatic temperature control, automatic dampness control and automatic ventilation control are met, the bioconversion rate is high, the fermentation period is short, the consumption is low, the cost is low, and mechanized and large-scale production requirements can be met.

The invention relates to a method for producing phycite by fermenting corn meal serving as a starting raw material with candida lipolytica, belonging to the field of microbial fermentation. The method comprises the following steps of: (1) mixing and extruding corn meal with separated and recycled yeast; (2) liquefying and saccharifying the material by undergoing an enzymatic hydrolysis reaction and fermenting phycite by inoculating fermentation seeds to obtain a phycite-containing fermentation liquid; and (3) degerming the fermentation liquid with a metal microfiltration membrane, separating the material from the liquid, and performing moisture evaporation concentration and crystallization on supernate by using a climbing film evaporator. The corn meal is directly taken as the raw material; and mixing, enzymatic hydrolysis and fermentation culturing are performed in the same fermentation tank, processes and devices for producing starch and glucose in the prior art are eliminated, repeated treatment of waste water produced in the production process and energy consumption are avoided, the flow is more economic and reasonable, and the resource utilization ratio can be effectively increased. By adopting a candida lipolytica strain with high phycite yield, screened with the method, the phycite yield can be increased by about 2 percent.

The invention relates to a straw feed produced by using a microbiological degradation technology, and aims at providing a straw feed with a good degradation effect and a production method of the straw feed. A microbiological strain solution used in the straw feed comprises 2.0*10<8>pfu / ml of white-rot fungi, 2.0*10<12>pfu / ml of lactobacillus acidophilus, 2.0*10<8>pfu / ml of trichoderma viride, 3.0*10<11>pfu / ml of bacillus subtilis and 5.0*10<12>pfu / ml of candida utilis according to the activity amount. The straw feed comprises the following components in parts by weight of 1.5-2kg of urea, 0.5kg of brown sugar, 0.1-0.2kg of composite trace elements and 0.02-0.06kg of composite vitamins in the terms of 100kg of straws; the microbiological strain solution is prepared according to the proportion that 0.05-0.15L of a substrate is fermented by 100kg of the microbiological strain solution; the urea, the brown sugar, the composite trace elements and the microbiological strain solution are mixed to prepare a feed liquid; the feed liquid is uniformly sprayed on the straws; and the straws are compressed into cube blocks of 50*50*80, and the cube blocks are sealed by using black plastic films, stacked and fermented for 30days to obtain the straw feed. The efficiency of fermenting and degrading straws is remarkably improved; and the fermented plastic is rich in nutritive values, and is beneficial to being digested and absorbed by cattle and sheeps.

A microbial ethyl esther sophorolipid derivative with no acetylated groups produced by Candida species, for treating and preventing sepsis / septic shock. The method of producing sophorolipids is through microbial resting cells of Candida bombicola. The sophorolipids obtained from resting state cultures are isolated as a complex mixture of compounds and then decanted as a dense oil from the culture broth, subsequently washed to remove free fatty acids. Secondary chemical transformation via base catalyzed hydrolysis is used to reduce the 8 possible structural sophorolipid species to a single moiety, the 17-L-[(2′-O-b-D-glucopyranosyl-b-D-glucopyranosyl)-oxy]-cis-9-octadecenoate de-acetylated free acid. The compound acts primarily through decreasing inflammatory cytokines and eliciting other synergistic anti-inflammatory mechanisms by blocking TLR4-CD14 upstream of the inflammatory signaling cascade. The compound can be administered either intraperitoneally or intravenously at single or multiple doses of 5-30 mg / kg of weight in solvent media or in capped nanoparticles, preferably within 48 hours of sepsis inception.

The invention discloses a preparation method of fermented rice bran feed and an application thereof. The preparation method of the fermented rice bran feed disclosed by the invention comprises the following steps: adding a complex microbial inoculant and a complex enzyme preparation into a fermentation raw material taking rice bran as a main component to obtain a fermentation system, and fermenting the fermentation system to obtain a fermentation product which is the fermented rice bran feed, wherein the active ingredients of the complex microbial inoculant are bacillus subtilis, bacillus licheniformis, lactobacillus plantarum, lactobacillus casei, enterococcus faecalis, saccharomyces cerevisiae and candida utilis; the active ingredients of the complex enzyme preparation are cellulase, acid protease, amylase, beta-mannase, xylanase and beta-glucanase. The shelf life of the obtained fermented rice bran feed is prolonged, and the production performance of mammals and the yield and quality of produced milk can be further improved by utilizing the obtained rice bran fermented feed.

The invention relates to a biological enhancer for ruminant feeds, and a preparation method for the biological enhancer. The biological enhancer comprises the following steps: (a) preparing a fermentation medium, and crushing and mixing 85 to 95 weight percent of a carbon source, 1 to 2 weight percent of a nitrogen source, 1 to 3 weight percent of a growth promoter and the balance of water for later use; (b) inoculating candida utilis into the fermentation medium obtained in step (a), and performing solid fermentation cultivation; (c) inoculating lactobacillus plantarum into the fermentation medium obtained in step (b), performing anaerobic solid fermentation cultivation, and performing sampling for OD600 at a time interval of 4h; (d) when OD600 enters an exponential phase, inoculating bacillus subtilis into the fermentation medium obtained in step (c), and performing solid fermentation cultivation. The biological feeds prepared according to the method have the advantages that the microecological balance of rumens of ruminants can be improved, the feed conversion rate can be increased, the immunity of the organism can be improved, and the products have special sour and sweet smell and high palatability, and are easy for animals to ingest.

A novel method of very efficiently elevating the yield of oligosacchrides containing α-galactosyl, compared with the conventional methods, which comprises treating galactose or a galactose-containing material with a specific α-galactosidase and thus performing a dehydrocondensation reaction at a high substrate concentration. Anti-candida compositions originating in foods, having a high safety and excellent anti-candida effect and not being restricted in the supply, which contain as the active ingredient oligosacchrides obtained by treating galactose or a galactose-containing material with an α-galactosidase and thus performing a dehydrocondensation.

The invention discloses bacterial and enzymic co-fermented traditional Chinese medicine fermented feed as well as a preparation method and an application thereof. The feed comprises probiotics including enterococcus faecium, bacillus subtilis, bacillus licheniformis, saccharomyces cerevisiae, candida utilis, aspergillus oryzae and sterile water, an enzymic preparation including xylanase, mannase and cellulase, traditional Chinese medicines including radix codcnopsitis pilosulas, radix astragali, flowers of poplar, smoked plums, fructus chebulae, rhizoma atractylodis, dried ginger, dried orangepeels and radix glycyrrhizae as well as raw material substrates including soybean meal, corn meal, fish meal and high-fat whey powder, the probiotics, the enzymic preparation and multiple traditionalChinese medicines are combined for co-fermentation, the efficacy is better played, and the fermented feed is added to conventional feed and can be used for preventing bacterial yellow and white scourof suckling pigs; and besides, the fermented feed contains rich natural acid metabolized by small peptides and probiotics, can help piglets digest and prevent feed diarrhea and is favorable for improving intake of the suckling pigs, particularly for preventing weight loss of weaning piglets, a good foundation is laid for later fattening stage of the piglets, and the problems caused by existing feed for the suckling pigs are solved.

The invention provides a preparation method for feed. The preparation method comprises the following steps: (a) mixing crushed crop straws and livestock feathers to obtain a mixture; (b) carrying out steam explosion treatment on the mixture to obtain a steam explosion material; (c) mixing the steam explosion material obtained by the step (b) with bran and nutrition liquid to obtain a culture medium; sequentially inoculating filamentous fungi, candida utilis and lactic acid bacteria on the culture medium and fermenting; (d) drying the culture medium fermented in the step (c) to obtain the feed. According to the preparation method for the feed provided by the invention, the feed is high in production efficiency, abundant in nutrient substances and easy to digest by livestock.

The invention belongs to the forage processing field, and relates to a making method of a tomato pomace fermented forage. The making method of the fermented forage comprises the following steps: uniformly stirring fermentation raw materials (a DM base) comprising 65-75% of a tomato pomace, 10-20% of wheat bran, 8-15% of cottonseed meal, 1.5-2.5% of urea and 1.5-2.5% of ammonium sulfate; inoculating Trichoderma koningii, Candida utilis and Saccharomyces cerevisiae according to a ratio of the Saccharomyces cerevisiae to the Candida utilis to the Saccharomyces cerevisiae of 1:1:1-1:2:2, wherein the inoculation amount accounts for 10-15% of a fermentation substrate, and the water content of the fermentation substrate is 45-55% (m / m); and carrying out shallow layer fermentation or submerged fermentation at 28-32DEG C for 2-4d while stirring once each 24h. The tomato pomace fermented forage can be directly used to feed ruminants, or is baked at 45-55DEG C to the water content of about 10% in order to obtain a tomato pomace fermented forage product. The fermentation method has the advantages of small energy consumption, short period and low cost, and the produced tomato pomace fermented forage has a crude protein content of 26% or more (the DM base) and a urea residual amount of 0.1% or less. The fermented forage has a good palatability and can be used for feeding the ruminants.

The invention discloses a method for increasing content of ethyl caproate in Luzhou-flavor liquor. The method comprises the following steps: preparing a bacterial solution A, preparing a bacterial solution B, and preparing a composite functional bacterial solution C; preparing reinforced Daqu D; and preparation of pit mud microorganism enrichment liquid E and fermentation in a pool. According to the method, bacillus siamensis and candida utilis are selected for culture and fermentation, reinforced yeast is prepared, and the content of an ethyl caproate precursor substrate in the pit is increased through the synergistic effect of the reinforced yeast and the pit mud microorganism enrichment liquid, so that synthesis of ethyl caproate is promoted. When the method is used for brewing the Luzhou-flavor liquor, the ethyl caproate content of the raw liquor produced by the new cellar and the aging cellar is obviously improved, the Luzhou-flavor style of the liquor body is prominent, and the aroma is mellow.

The invention relates to feed used for sea cucumber and a preparation method thereof. With sargassum thunbergii, gulfweed, kelp powder and bean pulp being basic raw materials, micro-ecological preparations are added for fermentation, then sea cucumber premix is added, and then the fermentation feed used for the sea cucumber is prepared. The fermentation feed is prepared from, by mass, the basic raw materials of 10-14% of sargassum thunbergii, 10-14% of gulfweed, 50-60% of kelp powder and 15-20% of bean pulp. The micro-ecological preparations are formed by mixing bacillus subtilis, candida utilis and trichoderma viride according to the proportion of 5-7:1-2:1-2, and the quantity of the added micro-ecological preparations accounts for 5-10% of the basic feed, and the sea cucumber premix accounts for 1-2% of the basic feed. The fermentation feed used for the sea cucumber has full aroma, has rich monosaccharide, disaccharide, amino acid, peptides and other micro-molecule nutrient substances and active probiotics easy to digest and absorb by the sea cucumber, increases the digestion and absorption rate of the feed for the sea cucumber, avoids waste of kelp biological resources and is provided as low-price and high-quality feed for the sea cucumber in the sea cucumber culture industry.

The invention discloses a method for producing a feed through pretreating at a high temperature and fermenting at a low temperature. The method comprises the following steps: first utilizing amylases, celluloses, hemicellulase, bacillus coagulans, bacillus licheniformis, tropophilous bacilli, proteus hause, chelating cocci, lignocellulolytic microorganisms and the like as degrading factors, and degrading starch, celluloses and hemicelluloses in the materials into monosaccharides or polysaccharides at 40-70 DEG C; then inoculating the materials with candida utilis, monilia tropicalis, saccharomyces cerevisiae, lactobacilli and bacillus subtilis by a fermenting technology, and fermenting at 20-40 DEG C to make a high-protein feed with oligomerization functional sugar for cattle, sheep, pigs, chicken or other animals. The method can solve the insufficient protein problem of animal husbandry, enhance animal constitutions, relieve the pressure that people and livestock contest with crop and reduce pollution, and has significant economical and social benefits.

The invention discloses a complex fungicide for improving feed fiber degradation rate and positively regulating rumen fermentation propionic acid generation as well as application thereof, belonging to the technical field of livestock and poultry feed. The complex fungicide contains bacillus subtilis, candida utilis and enterococcus faecium. According to the complex fungicide provided by the invention, the fiber digestibility of rumen microbial feed can be obviously improved, the total production amount of total rumen microbial fermentation volatile fatty acids is increased by 21.1 percent, and the positive regulation amount of gluconeogenesis precursor propionic acid production amount is 10.5-11.3 percent. The preparation method is simple and high in operability and is suitable for feed processing in livestock and poultry industry, and the ruminant feed energy utilization efficiency can be obviously improved.

The invention discloses a method for preparing selenium-enriched yeasts by using Candida utilis, which relates to a novel method for preparing selenium-enriched yeasts. The method for preparing the selenium-enriched yeasts comprises the following steps: a, reviving Candida utilis with a collection number of CICC 1422 and preparing seeds; b, inoculating the seeds obtained by the step a onto a fermentation medium to ferment the seeds for 24 to 36 hours; c, adding selenium into fermentation liquor till the concentration of the selenium is 5 to 20mg / L, continuing fermentation for 24 to 48 hours, and collecting bacterium solution; and d, centrifuging the bacterium solution obtained by the step c, collecting thalli, drying and obtaining the selenium-enriched yeasts. In the method, the production cost, the biomass of the selenium-enriched yeasts, the selenium content in the selenium-enriched yeast can be taken into better consideration at the same time, selenium-enriched yeasts with high biomass and high selenium content can be obtained under a condition of small inorganic selenium consumption, and the method is suitable for industrial production.

The invention discloses a method for producing protein feed material by enzymolysis and fermentation of guar meal, comprising the steps of: liquid culturing candida utilis fermentation liquor, bacillus natto fermentation liquor and lactobacillus plantarum fermentation liquor; preparing a mixed bacterium solution according to the proportion; putting guar meal into an explosion tank, and performing steam explosion under certain conditions; mixing the materials after steam explosion with the mixed bacterium solution and molasses according to the proportion, and meanwhile adding enzymatic preparations to prepare mixed materials; sending the mixed materials into a special modular solid state biological material continuous fermentation device for performing enzyme solution and fermentation, drying the fermented materials and thereby obtaining the protein feed material. According to the method, the modular automatic fermentation device is adopted, and after being mixed with the fermentation bacterium solution and the enzymatic preparations, the materials after explosion is put into the automatic fermentation device for performing enzyme solution and biological fermentation, so that the requirements of automatically controlling temperature, humidity and ventilation quantity are achieved, the biotransformation efficiency is high, the fermentation period is short, the loss is little, the costs are low, and the requirement of mechanized and large-scale production can be achieved.

The invention provides sweet potato dreg type fermented feed which is prepared through the fermentation technology of candida utilis and the like by using the abundant sweet potato dreg resources in areas where sweet potatoes are planted densely. According to the sweet potato dreg type fermented feed, the existing technology of fermented sweet potato dregs is improved, production parameters are made clear, production links are reduced, and the fermented sweet potato dregs with the higher feeding value are acquired.

The invention belongs to the technical field of biological fermentation, and discloses a biological fermentation preparation and a preparation method thereof. Zymophyte is activated and cultured and then inoculated into a solid fermentation culture medium composed of the citrus pulp, the green plum fruit extract, bran and urea for fermentation culture, and the citrus pulp biological fermentation preparation is obtained; the zymophyte is composed of aspergillus niger, candida tropicalis, lactobacillus plantarum and bacillus subtilis. Through combination of specific zymophyte and taking the citrus pulp as a main fermentation raw material, the obtained fermentation product is applied to broiler feeding, so that the growth performance of broilers can be improved by increasing the nutrient substance digestibility of the broilers and promoting intestinal development, Meanwhile, the function of improving the quality and flavor of meat products is also achieved.

The invention discloses a method for producing protein feed raw materials by carrying out enzymolysis and fermentation on white spirit vinasse. The method comprises the following steps of: culturing a candida tropicalis fermentation solution, a bacillus natto fermentation solution and a lactobacillus plantarum fermentation solution in liquid, and preparing into a mixed bacterium solution according to the ratio; filling the white spirit vinasse and CaO into an explosion cylinder according to the ratio; carrying out steam explosion under a certain condition; mixing exploded materials, the mixed bacterium solution and corn slurry at the ratio; meanwhile, adding an enzyme preparation to prepare a mixed material; and feeding the mixed material into a special modularized solid-state biological raw material continuous fermentation device to carry out enzymolysis and biological fermentation; and drying the fermented materials to obtain the protein feed raw materials. According to the method disclosed by the invention, the exploded materials, the fermented bacterium solution and the enzyme preparation are mixed and then are conveyed into the special modularized solid-state biological raw material continuous fermentation device to carry out the enzymolysis and biological fermentation, so that the requirements of automatic temperature control, humidity control and ventilation amount control are realized; the biological conversion rate is high, the fermentation period is short, the loss is less, the cost is low, and the mechanical and large-scale production can be realized.

The invention provides a fermented feed for fish. The fermented feed is prepared through performing mixed fermentation on raw materials, composite microbes A, composite microbes B and water, wherein the composite microbes A comprise one or more of bacillus subtilis, bacillus licheniformis, bacillus coagulans and candida utilis; and the composite microbes B comprise one or more of lactobacillus acidophilus, plant lactic acid bacteria and enterococcus faecalis. According to the fermented feed for fish, provided by the invention, a two-step fermentation method is adopted, the composite microbes Aare used for performing aerobic fermentation for 2-3 days at an early stage, the composite microbes B are added at a later stage, airtight fermentation is performed for 5-7 days, and the synergisticreaction of different strains is used for degrading macromolecular protein in the raw materials and reducing the content of anti-nutritional factors; besides, a large number of bioactive substances are produced, and the fermented feed which is high in nutrient value and easy to digest and utilize is obtained; and then other feed raw materials are added, a cold granulating making technology is usedfor cooperation, and the fermented cold granulated feed is prepared, so that active beneficial factors in the fermentation process are reserved to the maximum extent.

The present invention discloses a method for producing a laying hen complete feed through solid enzymolysis fermentation. The method comprises: carrying out liquid culture on Candida utilis, Bafillus natto and Lactobacillus plantarum; preparing a mixed bacterial liquid according to a certain ratio; loading corn, corn stalks and other raw materials into a blasting jar in a mixed manner according to a certain ratio, and carrying out steam blasting under a certain condition; mixing the blasted material and the mixed bacterial liquid according to a certain ratio while adding an enzyme preparation; and conveying the prepared mixed material into a special modular solid-state biological raw material continuous fermentation device, carrying out enzymolysis and fermentation, and drying the fermented material so as to obtain the laying hen complete feed. According to the method of the present invention, the modular automatic fermentation device is used, the blasted material, the fermented bacterial liquid and the enzyme preparation are mixed, the mixture is loaded into the modular solid-state biological raw material automatic fermentation device, and the enzymolysis and the biological fermentation are performed, such that the requirements of automatic temperature control, humidity control and ventilation control are achieved, the biological conversion rate is high, the fermentation period is short, the cost is low, and the mechanized and large-scale production needs can be met.

The invention belongs to the field of biological feed, and relates to a poultry feed additive containing fermented Chinese yew, and a preparation method and applications thereof. The poultry feed additive is a dried product obtained via fermentation of Chinese yew using a compound microbial agent, and the compound microbial agent is composed of lactobacillus plantarum fermentation broth, lactobacillus acidophilus fermentation broth, and candida utilis fermentation broth. The compound microbial agent is used for biodegradation of active ingredients of Chinese yew; treatment method is mild; the active ingredients of Chinese yew are protected as far as possible; poultry absorption rate is increased; a poultry feed prepared from the poultry feed additive contains Chinese yew ingredient, lactic acid bacteria, and saccharomycetes, and is capable of increasing poultry production efficiency; a Chinese yew feed obtained via microbial fermentation is mixed with poultry daily feed at a ratio of 1 to 5%, and an obtained mixture can be used for feeding after uniform stirring.

The invention discloses a production method of high-protein feed containing arginine. The production method comprises the following steps: performing liquid fermentation with corynebacterium glutamicum, candida utilis, lactobacillus plantarum and bacillus natto; mixing fermented mash rich in metabolic products with mash rich in thalluses, then mixing the mixture with soybean meal and the like in proportion, and performing solid state fermentation; finally drying products, and packaging the dried products so as to obtain final products containing crude protein of which the content is higher than 50% and arginine of which the content is higher than 4.0%. The production method disclosed by the invention has the advantages that the technology is simple, mixed fermentation is directly performed on the fermented mash and solid materials, the microbial metabolism product namely the arginine is utilized, so that the time of the solid state fermentation is greatly shortened, and the probability of infectious microbes infection in the solid state fermentation process of raw materials is reduced; the energy consumption is low, and waste is not discharged in the fermentation process; according to feed raw materials prepared by the production method, the content of anti-nutritional factors in a plant meal group is reduced, the content of the arginine is higher than 4.0%, the additive amount of exogenous arginine can be reduced or replaced, and the cost of the feed is reduced.

The invention discloses a method for producing protein feed raw materials by carrying out enzymolysis and fermentation on soybean meal. The method comprises the following steps of: culturing a candida utilis fermentation solution, a bacillus subtilis fermentation solution and a lactobacillus acidophilus fermentation solution in liquid, and preparing into a mixed bacterium solution according to the ratio; filling the soybean meal into an explosion cylinder; carrying out steam explosion under a certain condition; mixing exploded materials, the mixed bacterium solution and molasses at the ratio; meanwhile, adding an enzyme preparation to prepare a mixed material; feeding the mixed material into a special modularized solid-state biological raw material continuous fermentation device to carry out enzymolysis and biological fermentation; and drying the fermented materials to obtain the protein feed raw materials. According to the method disclosed by the invention, the exploded materials, the fermented bacterium solution and the enzyme preparation are mixed and then are conveyed into the special modularized solid-state biological raw material continuous fermentation device to carry out the enzymolysis and biological fermentation, so that the requirements of automatic temperature control, humidity control and ventilation amount control are realized; the biological conversion rate is high, the fermentation period is short, the loss is less, the cost is low, and the mechanical and large-scale production can be realized.

The invention discloses a method of producing stalk ethanol by means of mixed fermentation of transgenetic yeast. The transgenetic yeast can respectively degrade cellulose and xylan of stalks to glucose and xylose and ferment glucose and xylose to ethanol without additionally providing cellulase and xylanase. Degradation of cellulose and xylan and fermentation are synchronously carried out. The method disclosed by the invention has the advantages that by means of transgenetic saccharomyces cerevisiae, transgenetic pichia stipitis and transgenetic candida shehatae, synchronous enzyme hydrolysis-fermentation of a stalk cellulose raw material is realized to produce ethanol without additionally adding cellulase and xylanase, thereby greatly lowering the production cost of stalk cellulose ethanol and promoting the progress of replacing petroleum fuel by the stalk cellulose ethanol.