61 results about "[Candida] apicola" patented technology

The invention provides a diglyceride oil composition for reducing lactone as well as a product and a preparation method thereof, and relates to the technical field of functional grease processing. The preparation method of the diglyceride oil composition comprises the following steps: (1) mixing corn oil and high oleic acid peanut oil, adding glycerol and water, then adding rhizomucor miehei lipase and pseudomonas onions lipase, and reacting to obtain a material A; (2) mixing sea buckthorn seed oil, acer truncatum seed oil and rapeseed oil, adding glycerol and water, adding candida antarctica lipase B and rhizopus oryzae lipase, and reacting to obtain a material B; and (3) mixing the material A and the material B, heating and centrifuging, taking an upper-layer oil sample, and distilling under a vacuum condition to obtain the diglyceride oil composition. The diglyceride oil composition prepared by the invention has a good effect in the aspect of reducing the internal fat.

The invention discloses a bacterium-enzyme synergistic fermentation method for sweet potato residues, which can realize rapid fermentation and has high protein content of the fermented sweet potato residues. According to the method, high-temperature-resistant alpha-amylase, cellulase and pectinase are used for carrying out enzymolysis on the sweet potato residues to decompose the sweet potato residues into reducing sugar, cellulose is preliminarily decomposed, and rapid and accurate starch conversion and cellulose degradation are carried out; a multi-bacterium combination of efficient degradation fibers, candida utilis capable of producing mycoprotein, lactobacillus plantarum JM113, bacillus subtilis and aspergillus niger is used for fermentation, so that the mycoprotein level in the fermented sweet potato residues is further improved, the nutritional value and the in-vitro digestibility are improved, the feeding price of the fermented sweet potato residues is improved, and the cost problem caused by high-moisture treatment is reduced; therefore, the economic value of the sweet potato residues is comprehensively improved, and the method has practical significance in waste utilization of the sweet potato residues, reduction of protein feed cost, alleviation of waste residue environmental pollution and improvement of industrial economic benefits. The method is suitable for popularization and application in the technical field of biological fermentation.

The invention discloses an efficient baijiu brewing method based on a microbial fermentation technology, and belongs to the technical field of baijiu brewing. The efficient baijiu brewing method comprises the following steps: (1) preparing chitosan-sodium alginate composite microspheres; (2) immobilizing bacillus siamensis, candida utilis and pediococcus acidilactici in the chitosan-sodium alginate composite microspheres to obtain an immobilized microbial carrier; (3) adding the immobilized microbial carrier into a white spirit fermentation pit, and controlling the fermentation temperature to be 23-30 DEG C for fermentation; (4) controlling the fermentation process in stages, including an initial stage, a middle stage and a later stage; and (5) after fermentation is completed, distilling and ageing to obtain the finished product white spirit. According to the invention, a remarkable technical breakthrough is realized on the basis of a traditional white spirit brewing process, and a new thought is provided for technical upgrading of the white spirit industry.

The invention discloses a processing method of fermented cottonseed meal for replacing soybean meal. The method comprises the following steps: uniformly mixing cottonseed meal, feather meal, corn starch and water to obtain a fermentation substrate, adding a first-stage fermentation inoculant into the fermentation substrate for aerobic fermentation, adding a second-stage fermentation inoculant for anaerobic fermentation after fermentation is finished, and performing low-temperature drying and crushing on a fermentation product after fermentation is finished to obtain the cottonseed meal fermented feed. The first-stage fermentation inoculant comprises candida utilis, aspergillus oryzae, papain and cellulase; and the second-stage fermentation inoculant comprises lactobacillus reuteri and lactobacillus acidophilus. According to the method, a bacterium-enzyme synergistic two-step fermentation mode is adopted, bacterium-enzyme synergistic aerobic fermentation is carried out firstly, then anaerobic fermentation is carried out with compound probiotics, and the obtained cottonseed meal fermented feed is high in crude protein and amino acid content and good in palatability, can completely replace soybean meal, can effectively regulate fat metabolism and can improve the lean meat percentage of fattening pigs.

The invention discloses a soybean yellow serofluid fermentation product rich in aglycone type soybean isoflavone as well as a preparation method and application of the soybean yellow serofluid fermentation product, and relates to the technical field of microbial fermentation. The preparation method comprises the following steps: inoculating double yeasts into a soybean yellow serofluid culture medium rich in glucoside type soybean isoflavone, adjusting the pH value of the soybean yellow serofluid culture medium, and performing constant-temperature fermentation to obtain a soybean yellow serofluid fermentation product; the double yeast is prepared from Wickerhamomyces anomalus NNB-1 and Candida utilis LYY1 according to a proportion, the Wickerhamomyces anomalus NNB-1 is preserved in the China Center for Type Culture Collection on April 3, 2023, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M2023451. The invention further discloses a preparation method of the double yeast for the Wickerhamomyces anomalus strain capable of improving the yield of the Wickerhamomyces anomalus strain capable of improving the yield of the Wickerhamomyces anomalus strain capable of improving the yield of the Wickerhamomyces anomalus strain capable of improving the yield of the Wickerhamomyces anomalus strain. According to the present invention, the soybean yellow serofluid rich in glucoside type soybean isoflavone is adopted as the fermentation substrate, and the Wickerhamomyces anomalus NNB-1 and the Candida utilis LYY1 are subjected to double-yeast fermentation so as to significantly improve the content of daidzein, daidzein, genistein and other aglycone type soybean isoflavone in the soybean yellow serofluid fermentation product.

The present invention provides a method, application and product for preparing diglyceride with weight loss effect by enzymolysis, which belongs to the field of enzyme engineering technology. The method comprises the following steps: S1, mixing lipase, ammonium heptamolybdate tetrahydrate with water, adding glycerol precipitation, filtering, and obtaining an enzyme preparation; S2, mixing plant raw material oil, glycerol, fatty acid, sodium fatty acid with the enzyme preparation obtained in step S1, reacting under inert gas conditions, and obtaining an enzymolysate; S3, molecularly distilling the enzymolysate obtained in step S2 to obtain diglyceride; wherein the lipase is a mixture of Rhizomucor miehei lipase and Candida antarctica Aspergillus niger lipase. The present invention uses ammonium heptamolybdate tetrahydrate to load a specific lipase mixture, and at the same time optimizes the component types and ratios of the raw materials in the enzymolysis process, effectively improving the mass percentage content and diglyceride yield of enzymolysis production of diglyceride.

The invention relates to a protein feed prepared from biologically refined distiller's grains and a preparation method thereof, and relates to the technical field of bioengineering and feed resource development. The method comprises the following steps: (1) sequentially carrying out acid pretreatment and double-enzyme synergistic hydrolysis on the white spirit vinasse to obtain hydrolysate containing fermentable sugar; (2) adding a candida utilis yeast liquid and urea, and carrying out carbon-nitrogen synergistic liquid fermentation to obtain a fermentation liquid with the viable count of candida utilis greater than or equal to 4.0 * 10 < 8 > CFU / mL; and drying. According to the method, the white spirit vinasse is subjected to diluted acid high-pressure pretreatment and double-enzyme synergistic hydrolysis, then candida utilis is utilized for carbon-nitrogen synergistic liquid fermentation, efficient utilization of glucose and xylose is achieved, and the mycoprotein yield is larger than or equal to 0.70 g / L / h; according to the method, all-component high-value utilization of the distiller's grains is realized, and a process path with industrial production potential is provided for preparing the protein feed by biorefining the distiller's grains.

The invention discloses a microbial fermentation feed capable of improving disease resistance of broiler chickens and a preparation method of the microbial fermentation feed, and belongs to the technical field of feed preparation. Comprising the following raw materials in parts by weight: 50-60 parts of corn flour, 20-25 parts of soybean meal, 5-8 parts of fish meal, 8-12 parts of tea seed cakes, 5-8 parts of mulberry leaves, 3-5 parts of dandelions, 0.1-0.3 part of zinc sulfate, 0.05-0.1 part of allicin and 0.5-1 part of a compound microbial agent. Wherein the compound microbial agent is prepared from lactobacillus plantarum, bacillus subtilis, candida utilis and bacillus licheniformis. The raw materials in parts by weight are mixed and fermented to obtain the microbial fermented feed capable of improving the disease resistance of the broiler chickens. According to the microbial fermentation feed disclosed by the invention, low-cost natural raw materials (such as tea seed cakes, mulberry leaves and dandelions) and the compound microbial agent are comprehensively utilized, so that the disease resistance of broilers can be comprehensively improved on the basis of reducing the cost; besides, the compound microbial agent can be fully fermented, the content of functional substances such as short-chain fatty acid (SCFAs) and antibacterial peptide is increased, the intestinal microecology can be effectively regulated and controlled, the immune function can be effectively enhanced, and the survival rate and disease resistance of the broiler chickens are improved.

The invention provides plant fermented oil with moistening and soothing effects and a preparation method thereof. The preparation method comprises the following steps: fermenting aspergillus in a fermentation culture medium 1, adding candida albicans, inoculating into a fermentation culture medium 2, carrying out primary fermentation under the ventilation capacity of 2-4 L / min, carrying out secondary fermentation under the ventilation capacity of 0.2-0.5 L / min, separating, and sterilizing to obtain the plant fermented oil. The aspergillus and candida mellifera are mixed and fermented according to a specific sequence, so that the obtained plant fermented oil has excellent soothing and moistening effects. Besides, compared with products obtained through fermentation in other modes, the plant fermentation oil has more excellent soothing and moistening effects and is very suitable for being used as and / or used for preparing cosmetics or medical compositions.

This invention relates to a protein feed prepared from biorefined baijiu lees and its preparation method, belonging to the fields of bioengineering and feed resource development technology. It includes the following steps: (1) subjecting the baijiu lees to acid pretreatment and dual-enzyme synergistic hydrolysis sequentially to obtain a hydrolysate containing fermentable sugars; (2) adding Candida utilis culture and urea for carbon-nitrogen synergistic liquid fermentation to obtain a Candida utilis viable count ≥4.0 × 10⁻⁶. 8 Fermentation broth with CFU / mL; drying is then performed. This invention pretreats baijiu (Chinese liquor) lees under high pressure with dilute acid, followed by synergistic hydrolysis with two enzymes, and then utilizes *Candida utilis* for carbon-nitrogen synergistic liquid fermentation, achieving efficient utilization of glucose and xylose, with a cell protein yield ≥0.70 g / L / h. This invention realizes the high-value utilization of all components of baijiu lees, providing a process route with industrial-scale production potential for the biorefining of baijiu lees to prepare protein feed.

This invention belongs to the field of alcoholic beverage processing technology, specifically relating to a high-purity ginkgo wine and its preparation method. The preparation method comprises the following steps: ginkgo slurry undergoes preliminary enzymatic hydrolysis with neutral protease and amylase, followed by secondary enzymatic hydrolysis with pectinase and sodium bisulfite; brewing yeast fermentation broth is added for pre-fermentation; glutamine transaminase, *Saccharomyces cerevisiae* fermentation broth, and *Candida valerate* fermentation broth are added for post-fermentation; and finally, distillation and filtration are performed to obtain the ginkgo wine. This invention achieves complete decomposition and fermentation of ginkgo kernels through a two-step enzymatic hydrolysis and two-step fermentation process. The preparation method has advantages such as simple process and low cost, which is beneficial for industrial production; the prepared ginkgo wine has advantages such as clarity, strong aroma, high alcohol content, and low acidity.

The invention belongs to the technical field of microorganisms, and particularly relates to a compound fermentation inoculant for increasing the relative content of ethyl decanoate in roxburgh rose fruit wine and application. According to the present invention, two saccharomycetes are separated from a wild rosa roxburghii fermentation broth, i.e., a Starmerella bacillus and a Saccharomyces cerevisiae, and are compounded to obtain the composite fermentation bacterial agent, and the bacterial agent can produce ethyl decanoate at high yield and is applied to rosa roxburghii fruit wine brewing, such that the relative content of ethyl decanoate in the rosa roxburghii fruit wine is increased, and the yield of the rosa roxburghii fruit wine is increased; the flavor quality of the roxburgh rose fruit wine is improved, and technical support is provided for industrial development of the roxburgh rose fruit wine. When the composite microbial agent is used for fermenting the rosa roxburghii tratt fruit wine, the flavor and taste of the rosa roxburghii tratt fruit wine can be remarkably improved, the relative content of ester substances in volatile flavor substances of the rosa roxburghii tratt fruit wine is increased by 68.527% in total, and the highest relative content of ethyl decanoate is 26.551%. In addition, the relative content of ethyl laurate and the relative content of ethyl caprylate are increased to 14.228% and 11.335% respectively.

The present disclosure provides a method for producing chemicals using halotolerant yeast. The method includes culturing halotolerant yeast cells in a medium comprising a solution of salts at a concentration of at least 0.5 times the salinity of seawater, and allowing the halotolerant yeast cells to produce one or more chemicals. The medium may include a carbon source, nitrogen source and / or phosphate source, trace elements, and additives. The halotolerant yeast cells may be selected from, e.g., Debaryomyces subglobosus, Candida taylori, Pichia anomaly Millerozyma farinosa, Blastobotrys adeninivorans, Scheffersomyces stipitis. or combinations thereof. The method enables chemical production in high-salt media, reducing freshwater usage in fermentation processes.

The invention relates to the technical field of microorganisms, in particular to Devodia sp. And a method for fermenting corn steep liquor by using the Devodia sp. The invention provides the Deworth's bacteria capable of efficiently degrading vomitoxin in the corn steep liquor, the Candida krusei and the Candida krusei are used for cooperatively fermenting the corn steep liquor, the pH of the corn steep liquor can be increased, the content of vomitoxin and antinutritional factors in the corn steep liquor can be reduced, the content of choline in the corn steep liquor is remarkably increased, the quality of the corn steep liquor is obviously improved, and the yield of the corn steep liquor is increased. The corn steep liquor subjected to synergistic fermentation can be better applied to the field of animal feed and has a wide application prospect.

The present invention discloses an antioxidant composition, a preparation method thereof, a cosmetic, and an application. The antioxidant composition comprises the following components by weight: 0.1 - 2 parts of glutathione disulfide, 1 - 8 parts of olive extract, 0.1 - 2 parts of taurine sulfonate, 1 - 5 parts of Candida utilis / glucose / methyl rapeseedate fermentation product, 5 - 10 parts of vegetable oil, 20 - 50 parts of polyol, and 23 - 72.8 parts of water. The antioxidant composition of the present invention can be stored for a long time, is not easily discolored or inactivated, has significant antioxidant and whitening effects, and has a significant inhibitory effect on superoxide anion, hydroxyl radical, DHHP, and ROS. It also has excellent antioxidant stability and can be used as an additive in skin care products.

The invention relates to the technical field of microbial fermentation, in particular to a preparation method and application of a digestible biological protein fermentation material. According to the fermentation method, candida utilis serves as a fermentation strain, industrial and agricultural waste glucose mother liquor and corn steep liquor serve as substrates, high-yield mycoprotein is obtained under the relative low-temperature fermentation condition of 20-25 DEG C, and the obtained fermentation liquor is subjected to single enzymolysis, so that a fermentation material easier to digest is obtained. When weaned piglets are fed with the obtained fermented material, the diarrhea rate of the piglets can be effectively reduced.

The present invention belongs to the field of food processing technology, and discloses a kind of rice bran fresh wet noodles and preparation method thereof. According to mass percentage, the raw material components of rice bran fresh wet noodles are rice bran 25-30%, chia seed powder 3-6%, white tea powder 4-7%, wheat flour 55-65%, salt 0.5-1.5%, a first composite bacterial agent 1.5-2%, and a second composite bacterial agent 1-1.5%; wherein, the first composite bacterial agent is obtained by mixing Lactobacillus acidophilus, Lactobacillus fermentum, and Candida utilis in a mass ratio of 3-6:8-12:2-4; the second composite bacterial agent is obtained by mixing Saccharomyces cerevisiae and Lactobacillus plantarum in a mass ratio of 1-4:3-10. The rice bran fresh wet noodles of the present invention are bright in color and light yellow. Compared with the existing fresh wet noodles, the nutrient content is significantly increased. It is not easy to break and will not become mushy during cooking. It has a more delicate and smooth taste, good viscoelasticity and chewiness, and shows better quality.

The invention belongs to the technical field of food processing, and discloses fine broken rice wet noodles and a preparation method thereof. The fresh wet fine broken rice noodles comprise the following raw material components in percentage by mass: 25-30% of fine broken rice, 3-6% of chia seed powder, 4-7% of white tea powder, 55-65% of wheat flour, 0.5-1.5% of table salt, 1.5-2% of a first complex microbial inoculant and 1-1.5% of a second complex microbial inoculant, wherein the first complex microbial inoculant is obtained by mixing lactobacillus acidophilus, lactobacillus fermentum and candida utilis according to the mass ratio of (3-6): (8-12): (2-4); the second complex microbial inoculant is obtained by mixing saccharomyces cerevisiae and lactobacillus plantarum according to the mass ratio of (1-4): (3-10). The fine broken rice fresh wet noodles are bright in color and light yellow, and compared with existing fresh wet noodles, the fine broken rice fresh wet noodles are obviously increased in nutrient substance content, not prone to breakage during cooking, free of soup burning, finer and smoother in taste, better in viscoelasticity and chewiness and better in quality.

The present application relates to high protein fermentation technical field, specifically disclose a kind of preparation method for fermenting production of yeast protein based on alcohol waste liquor, to solve the technical problems such as low utilization rate of alcohol waste liquor resource, feed soybean meal supply is nervous and high cost.The method includes the following steps: first, the alcohol waste liquor produced by grain deep processing is concentrated to dry basis content ≥30%, the composite carbon source carrier is crushed, urea and molasses are added to adjust the carbon-nitrogen ratio to (20~25):1, access to the complex fermentation strain containing Candida utilis, solid fermentation is carried out, and the finished product yeast protein is obtained.The present application uses solid fermentation mode, matches composite carbon source carrier to optimize nutrient ratio, step by step controls key process parameters, and the finished product yeast protein is ≥24%, true protein is ≥22%, and nitrogen conversion rate is ≥85%, which can replace soybean meal as feed additive.The method realizes alcohol waste liquor resource utilization, pollution is small, reduces feeding cost, has environmental protection and economic value, and is suitable for industrialization popularization and application.

According to the tomato pomace fermented protein feed and the preparation method thereof provided by the invention, tomato pomace and bran are used as fermentation substrates, candida utilis and pichia pastoris are used as fermentation strains, fermentation processes such as strain inoculum size, fermentation time and enzyme addition amount are further optimized, and under optimal fermentation process conditions, the tomato pomace fermented protein feed is prepared. The content of crude protein in the obtained tomato peel residues is 35.93%. After fermentation, the crude protein content of the tomato peel residues is increased by 10.59%, and hemicellulose is reduced by 4.81%. The tomato pomace is cooperatively fermented by bacteria and enzymes, so that the cellulose content of the tomato pomace is reduced, the protein content of the tomato pomace is increased, the tomato pomace can be subsequently used for feeding monogastric livestock and poultry, and the method has great significance in realizing resource utilization of wastes, reducing environmental pollution and relieving waste treatment pressure faced by tomato processing enterprises. The nutritional value of the feed can be improved, the breeding cost is reduced, and sustainable development of animal husbandry is promoted.

The present invention relates to the technical field of microbial fermentation, and discloses a method for producing selenium-rich protein feed by multi-strain co-fermentation of Hami melons. The Hami melons are crushed, then mixed with a carbon source and a nitrogen source, and then inoculated with a fermentation agent for fermentation; the fermented product is dried and pulverized to obtain a protein feed; the fermentation agent includes Candida utilis, Aspergillus niger, and Bacillus licheniformis; the components of the Candida utilis culture medium include yeast extract 9.56 g / L, glucose 19.86 g / L, dipotassium hydrogen phosphate 1.45 g / L, magnesium sulfate 1.12 g / L, sodium selenite 25 mg / L, L-lysine and vitamin B1. In terms of mass concentration, the ratio of sodium selenite, L-lysine and vitamin B1 is 1:(116-232):(0.06-0.12). In the present invention, multi-strain co-fermentation of Hami melons is adopted to obtain a selenium-rich protein feed.

The invention belongs to the crossing field of cultivation and bioengineering, and particularly relates to a multi-strain insect protein and microalgae compounded bullfrog feed and a preparation method thereof, the multi-strain insect protein and microalgae compounded bullfrog feed comprises the following components: 25-45% of fermented insect protein, 15-30% of DHA microalgae powder, 10-20% of an auxiliary protein source, 0.5-2% of compound vitamin selected from fermented soybean meal or enzymolysis cottonseed protein, 0.5-2% of vitamin B, 0.5-2% of vitamin C, 0.5-2% of vitamin C, 0.5-2% of vitamin C, 0.5 1%-3% of composite mineral substances, 2%-5% of an adhesive and the balance of carbohydrate; the total DHA content of the bullfrog feed is larger than or equal to 1.5% by dry weight, the ratio of omega-3 fatty acid to omega-6 fatty acid is maintained to be 1: 1-1: 3, and the bullfrog feed has the advantages that saccharomyces cerevisiae is replaced with candida utilis, so that the capability of decomposing insect chitin is enhanced; chlorella is used for supplementing chlorophyll and algal polysaccharides to form a cell protection synergistic effect with schizochytrium limacinum, and DHA is supplemented at the same time; under the action of alkaline protease secreted by bacillus subtilis, the proportion of a beta-folding structure is reduced from 32% to 18%, and the digestibility is remarkably improved.

The invention provides a method for preparing ruminant biological feed through fermentation of sodium humate and probiotics, and relates to the field of solid feed fermention.The method comprises the steps that S1, candida utilis powder, lactobacillus plantarum liquid, enterococcus faecium liquid and beet molasses are stirred and mixed, and molasses bacterial liquid is obtained; s2, mixing the molasses bacterial liquid with the treated sodium humate and gelatinized starch urea according to a preparation ratio, and uniformly stirring; and S3, loading into an anaerobic sealed fermentation bag, and naturally fermenting in a greenhouse at the temperature of 25-35 DEG C for 30-48 hours. The biological feed which is rich in high-activity probiotics, high in protein and easy to digest and absorb by animals is prepared from byproducts such as sodium humate, molasses and the like by utilizing biological engineering, and can replace antibiotics in the feed.

The application provides a vinegar residue-based single-cell protein feed and a preparation method and application thereof, and belongs to the technical field of feed preparation.The application uses vinegar residue as raw material, adopts solid-state fermentation of Aspergillus, Candida utilis and Saccharomyces cerevisiae in combination with composite enzymes, utilizes the synergistic effect among the strains and the enzymolysis effect of the exogenous enzymes, efficiently degrades the fibrous substances in the vinegar residue, and converts the fibrous substances into microbial proteins, so that the crude protein content of the prepared vinegar residue-based single-cell protein feed is increased from 5% to 8% of the raw material to 20% to 25%, and the total fibrous substance degradation rate is greater than or equal to 60%. The vinegar residue-based single-cell protein feed produced by the application has good feeding value, can effectively replace part of the protein raw materials such as soybean meal, improves the production performance of beef cattle, reduces the breeding cost, realizes high-value utilization of vinegar residue resources, and has good popularization and application prospect.

The invention discloses a formula of a fermented feed for enhancing animal immune function, and relates to the technical field of fermented feeds. The formula comprises the following components in parts by mass: a basic raw material, an immune enhancement extract, a functional additive, 0.3-0.6 part of a composite microbial agent and 2-4 parts of composite mineral substances. Wherein the ganoderma lucidum extract and the folium cortex eucommiae extract activate immune cells through the synergistic effect of polysaccharide and chlorogenic acid; the complex microbial inoculant is composed of lactobacillus acidophilus, bacillus licheniformis and candida utilis according to a ratio of (5-6): (3-4): 2, efficiently degrades antinutritional factors and generates immunocompetence metabolites. The preparation process comprises the following steps: supercritical extraction of plant active components, mixing and humidifying of base materials, segmented stirring and fermentation after microbial inoculum activation, and then addition of functional components and drying. Through synergistic combination of the raw materials and process optimization, the animal immunity is remarkably improved, and the feed additive has the advantages of low cost and multiple immune enhancement mechanisms.

The invention relates to the field of livestock and poultry breeding, and discloses a laying hen feed additive based on fermented broussonetia papyrifera and application of the laying hen feed additive. The additive is prepared by taking paper mulberry leaves as a main material, matching with rice husks, wheat bran and other auxiliary materials, inoculating a microecological preparation containing lactobacillus plantarum, candida utilis and bacillus subtilis, and performing anaerobic fermentation. 4% of the additive is used for daily ration of the aged laying hens, so that the laying rate can be remarkably increased, the dirty egg rate can be reduced, and the egg weight, the eggshell strength and the yolk color can be improved; the mechanism is to improve the form of the intestinal tract, enhance the expression of tight junction protein, regulate immune balance, optimize the structure of cecum flora and enrich beneficial microorganisms; the broussonetia papyrifera leaf feed additive realizes efficient utilization of broussonetia papyrifera leaf resources, provides a safe and effective functional additive for prolonging the laying period of laying hens and improving the breeding benefits, and has a good application prospect.

The invention discloses a preparation method and application of a rice fermentation product, and the preparation method comprises the following steps: S1, mixing rice, cold water and glucose according to a weight ratio of 1: (15-25): (0.01-0.03), soaking for 8 hours, and performing autoclaved sterilization at 100-121 DEG C for 10-20 minutes; s2, a composite yeast seed solution is inoculated into the sterilized rice mixed solution, the composite yeast seed solution is composed of candida utilis and Michelia yeast according to the weight ratio of (1-2): 1, and the weight ratio of rice to the composite yeast seed solution is (20-30): 1; s3, fermenting for 24-72 hours under the conditions of non-sealing, constant temperature of 26-30 DEG C and oscillation speed of 150 rpm / min, and controlling the pH value to be 4.5-6.0; s4, performing high-pressure microjet homogenization on the fermentation product at the rotating speed of 6-10rpm for 10-20 minutes; and S5, centrifuging the homogenized liquid for 30 minutes at 4000 rpm, collecting supernate, sterilizing for 20 minutes at 120 DEG C, filtering by using a microporous membrane of 0.45 mu m, and adding p-hydroxyacetophenone which accounts for 0.3-0.5% of the total weight of the filtrate and 1, 2-hexanediol which accounts for 0.5-1% of the total weight of the filtrate.

The present invention provides stinky tofu and a preparation technology thereof, and belongs to the technical field of food processing, and the preparation technology comprises the following steps: S1, preparation of acid water: taking Chinese yams, jujubes and radix puerariae for enzymolysis saccharification to obtain an enzymolysis product, taking poria cocos, dried orange peels and honeysuckles for water extraction to obtain a water extract, mixing the enzymolysis product with the water extract, inoculating a complex microbial inoculant, and performing fermentation to obtain the acid water; performing temperature-controlled fermentation to obtain acid water; the complex microbial inoculants are lactobacillus plantarum, streptococcus thermophilus and candida utilis; s2, preparing bean curd: cleaning, soaking and grinding soybeans, then filtering to obtain soybean milk, heating the soybean milk, adding acid water, and carrying out curdling, blank pressing and cutting to obtain bean curd blocks; s3, preparation of the stinky tofu: preparing brine, soaking the tofu blocks in the brine for standing, draining the soaked tofu blocks, frying, seasoning and packaging to obtain the stinky tofu. The invention provides the stinky tofu and the preparation process thereof, and aims of shortening the process period and increasing the flavor level of the stinky tofu are fulfilled.

The invention relates to a method for producing a single-cell protein feed through synergy of enzymes and bacteria. The method comprises the following steps: adding a crushed straw raw material into compound cellulase compounded by endoglucanase, exonuclease and beta-glucosidase for enzymolysis; adding aspergillus oryzae and trichoderma longibrachiatum into the raw materials subjected to enzymolysis for fermentation; and then adding candida utilis for continuous fermentation to obtain the single-cell protein feed. The method has the advantages of short production time, high protein yield of the single-cell protein feed, low condition requirements in the production process, low production cost and the like.

The present application relates to a protein feed containing a composite fermentation composition, and belongs to the technical field of microbial fermentation and feed.The protein feed comprises fermentation strains and fermentation substrates; the fermentation strains comprise Rhodopseudomonas palustris with a preservation number of CGMCC NO.17021 and Candida glabrata with a preservation number of CCTCC NO:M2015664; and the fermentation substrates comprise cottonseed meal powder, rapeseed meal powder, distiller's grains powder and citrus dreg powder.The protein feed containing the composite fermentation composition has a high feed utilization rate and can improve the immunity of the animals fed with the protein feed.