88 results about "[Candida] apicola" patented technology

The present invention relates to the field of food technology, and in particular to a method for preparing diglyceride and its application. The method comprises the following steps: firstly hydrolyzing the oil and fat under the action of a first lipase, adding a solubilizing agent, and obtaining a hydrolyzate; then mixing the hydrolyzate with glycerol, and performing an esterification reaction with a second lipase; wherein the first lipase is prepared by compounding lipase Lipozyme RM and Antarctic Candida lipase B. The present invention can specifically synthesize 1,3-diglyceride that is beneficial to the cardiovascular system of the body, and has a high yield and good application prospects.

The invention provides a diglyceride oil composition for reducing lactone as well as a product and a preparation method thereof, and relates to the technical field of functional grease processing. The preparation method of the diglyceride oil composition comprises the following steps: (1) mixing corn oil and high oleic acid peanut oil, adding glycerol and water, then adding rhizomucor miehei lipase and pseudomonas onions lipase, and reacting to obtain a material A; (2) mixing sea buckthorn seed oil, acer truncatum seed oil and rapeseed oil, adding glycerol and water, adding candida antarctica lipase B and rhizopus oryzae lipase, and reacting to obtain a material B; and (3) mixing the material A and the material B, heating and centrifuging, taking an upper-layer oil sample, and distilling under a vacuum condition to obtain the diglyceride oil composition. The diglyceride oil composition prepared by the invention has a good effect in the aspect of reducing the internal fat.

The invention discloses a bacterium-enzyme synergistic fermentation method for sweet potato residues, which can realize rapid fermentation and has high protein content of the fermented sweet potato residues. According to the method, high-temperature-resistant alpha-amylase, cellulase and pectinase are used for carrying out enzymolysis on the sweet potato residues to decompose the sweet potato residues into reducing sugar, cellulose is preliminarily decomposed, and rapid and accurate starch conversion and cellulose degradation are carried out; a multi-bacterium combination of efficient degradation fibers, candida utilis capable of producing mycoprotein, lactobacillus plantarum JM113, bacillus subtilis and aspergillus niger is used for fermentation, so that the mycoprotein level in the fermented sweet potato residues is further improved, the nutritional value and the in-vitro digestibility are improved, the feeding price of the fermented sweet potato residues is improved, and the cost problem caused by high-moisture treatment is reduced; therefore, the economic value of the sweet potato residues is comprehensively improved, and the method has practical significance in waste utilization of the sweet potato residues, reduction of protein feed cost, alleviation of waste residue environmental pollution and improvement of industrial economic benefits. The method is suitable for popularization and application in the technical field of biological fermentation.

The invention discloses an efficient baijiu brewing method based on a microbial fermentation technology, and belongs to the technical field of baijiu brewing. The efficient baijiu brewing method comprises the following steps: (1) preparing chitosan-sodium alginate composite microspheres; (2) immobilizing bacillus siamensis, candida utilis and pediococcus acidilactici in the chitosan-sodium alginate composite microspheres to obtain an immobilized microbial carrier; (3) adding the immobilized microbial carrier into a white spirit fermentation pit, and controlling the fermentation temperature to be 23-30 DEG C for fermentation; (4) controlling the fermentation process in stages, including an initial stage, a middle stage and a later stage; and (5) after fermentation is completed, distilling and ageing to obtain the finished product white spirit. According to the invention, a remarkable technical breakthrough is realized on the basis of a traditional white spirit brewing process, and a new thought is provided for technical upgrading of the white spirit industry.

The invention provides a method for improving the conversion efficiency of fermentation protein for a cyperus esculentus feed and the prepared fermentation protein for the cyperus esculentus feed. Comprising the following steps: uniformly mixing cyperus esculentus cake meal, chitosan oligosaccharide and water, inoculating a fermentation strain, and culturing under proper conditions. Wherein the fermentation strain comprises saccharomycetes or a mixed strain of saccharomycetes and aspergillus niger; the saccharomycetes comprise saccharomyces cerevisiae or a mixed strain of the saccharomyces cerevisiae and candida utilis saccharomycetes. Saccharomyces cerevisiae is used as a main fermentation strain, sugar in fermentation raw materials can be fully utilized, mycoprotein is generated, and the protein content is increased; the candida utilis can utilize various carbon sources and nitrogen sources, so that the protein content and the thallus biomass are further improved; aspergillus niger can generate saccharifying enzyme, starch is hydrolyzed into glucose and other simple saccharides, and the saccharifying process is promoted. According to the method provided by the invention, the cyperus esculentus raw material with relatively low protein content can be converted into the cyperus esculentus feed fermentation protein, and the utilization way of the cyperus esculentus is expanded.

The invention discloses a processing method of fermented cottonseed meal for replacing soybean meal. The method comprises the following steps: uniformly mixing cottonseed meal, feather meal, corn starch and water to obtain a fermentation substrate, adding a first-stage fermentation inoculant into the fermentation substrate for aerobic fermentation, adding a second-stage fermentation inoculant for anaerobic fermentation after fermentation is finished, and performing low-temperature drying and crushing on a fermentation product after fermentation is finished to obtain the cottonseed meal fermented feed. The first-stage fermentation inoculant comprises candida utilis, aspergillus oryzae, papain and cellulase; and the second-stage fermentation inoculant comprises lactobacillus reuteri and lactobacillus acidophilus. According to the method, a bacterium-enzyme synergistic two-step fermentation mode is adopted, bacterium-enzyme synergistic aerobic fermentation is carried out firstly, then anaerobic fermentation is carried out with compound probiotics, and the obtained cottonseed meal fermented feed is high in crude protein and amino acid content and good in palatability, can completely replace soybean meal, can effectively regulate fat metabolism and can improve the lean meat percentage of fattening pigs.

The invention discloses a soybean yellow serofluid fermentation product rich in aglycone type soybean isoflavone as well as a preparation method and application of the soybean yellow serofluid fermentation product, and relates to the technical field of microbial fermentation. The preparation method comprises the following steps: inoculating double yeasts into a soybean yellow serofluid culture medium rich in glucoside type soybean isoflavone, adjusting the pH value of the soybean yellow serofluid culture medium, and performing constant-temperature fermentation to obtain a soybean yellow serofluid fermentation product; the double yeast is prepared from Wickerhamomyces anomalus NNB-1 and Candida utilis LYY1 according to a proportion, the Wickerhamomyces anomalus NNB-1 is preserved in the China Center for Type Culture Collection on April 3, 2023, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M2023451. The invention further discloses a preparation method of the double yeast for the Wickerhamomyces anomalus strain capable of improving the yield of the Wickerhamomyces anomalus strain capable of improving the yield of the Wickerhamomyces anomalus strain capable of improving the yield of the Wickerhamomyces anomalus strain capable of improving the yield of the Wickerhamomyces anomalus strain. According to the present invention, the soybean yellow serofluid rich in glucoside type soybean isoflavone is adopted as the fermentation substrate, and the Wickerhamomyces anomalus NNB-1 and the Candida utilis LYY1 are subjected to double-yeast fermentation so as to significantly improve the content of daidzein, daidzein, genistein and other aglycone type soybean isoflavone in the soybean yellow serofluid fermentation product.

The present invention provides a method, application and product for preparing diglyceride with weight loss effect by enzymolysis, which belongs to the field of enzyme engineering technology. The method comprises the following steps: S1, mixing lipase, ammonium heptamolybdate tetrahydrate with water, adding glycerol precipitation, filtering, and obtaining an enzyme preparation; S2, mixing plant raw material oil, glycerol, fatty acid, sodium fatty acid with the enzyme preparation obtained in step S1, reacting under inert gas conditions, and obtaining an enzymolysate; S3, molecularly distilling the enzymolysate obtained in step S2 to obtain diglyceride; wherein the lipase is a mixture of Rhizomucor miehei lipase and Candida antarctica Aspergillus niger lipase. The present invention uses ammonium heptamolybdate tetrahydrate to load a specific lipase mixture, and at the same time optimizes the component types and ratios of the raw materials in the enzymolysis process, effectively improving the mass percentage content and diglyceride yield of enzymolysis production of diglyceride.

The invention relates to a protein feed prepared from biologically refined distiller's grains and a preparation method thereof, and relates to the technical field of bioengineering and feed resource development. The method comprises the following steps: (1) sequentially carrying out acid pretreatment and double-enzyme synergistic hydrolysis on the white spirit vinasse to obtain hydrolysate containing fermentable sugar; (2) adding a candida utilis yeast liquid and urea, and carrying out carbon-nitrogen synergistic liquid fermentation to obtain a fermentation liquid with the viable count of candida utilis greater than or equal to 4.0 * 10 < 8 > CFU / mL; and drying. According to the method, the white spirit vinasse is subjected to diluted acid high-pressure pretreatment and double-enzyme synergistic hydrolysis, then candida utilis is utilized for carbon-nitrogen synergistic liquid fermentation, efficient utilization of glucose and xylose is achieved, and the mycoprotein yield is larger than or equal to 0.70 g / L / h; according to the method, all-component high-value utilization of the distiller's grains is realized, and a process path with industrial production potential is provided for preparing the protein feed by biorefining the distiller's grains.

The invention discloses a microbial fermentation feed capable of improving disease resistance of broiler chickens and a preparation method of the microbial fermentation feed, and belongs to the technical field of feed preparation. Comprising the following raw materials in parts by weight: 50-60 parts of corn flour, 20-25 parts of soybean meal, 5-8 parts of fish meal, 8-12 parts of tea seed cakes, 5-8 parts of mulberry leaves, 3-5 parts of dandelions, 0.1-0.3 part of zinc sulfate, 0.05-0.1 part of allicin and 0.5-1 part of a compound microbial agent. Wherein the compound microbial agent is prepared from lactobacillus plantarum, bacillus subtilis, candida utilis and bacillus licheniformis. The raw materials in parts by weight are mixed and fermented to obtain the microbial fermented feed capable of improving the disease resistance of the broiler chickens. According to the microbial fermentation feed disclosed by the invention, low-cost natural raw materials (such as tea seed cakes, mulberry leaves and dandelions) and the compound microbial agent are comprehensively utilized, so that the disease resistance of broilers can be comprehensively improved on the basis of reducing the cost; besides, the compound microbial agent can be fully fermented, the content of functional substances such as short-chain fatty acid (SCFAs) and antibacterial peptide is increased, the intestinal microecology can be effectively regulated and controlled, the immune function can be effectively enhanced, and the survival rate and disease resistance of the broiler chickens are improved.

The invention relates to the technical field of animal feed, in particular to fermented rapeseed meal as well as a preparation method and application thereof. The present invention provides a fermented rapeseed meal preparation method, which comprises: mixing a rapeseed meal-containing fermentation raw material, an enzyme preparation and a first fermentation bacterial agent, carrying out aerobic fermentation, and after the aerobic fermentation is completed, adding a second fermentation bacterial agent to carry out anaerobic fermentation, the first fermentation inoculant comprises aspergillus niger, aspergillus oryzae, bacillus subtilis and bacillus amyloliquefaciens; the second fermentation microbial agent comprises candida utilis and lactobacillus plantarum. According to the method, the content of toxins and antinutritional factors in the rapeseed meal is effectively reduced, meanwhile, the content of crude protein in the fermented rapeseed meal and the digestion efficiency and metabolizable protein content of nutrient substances such as dry matter and protein in rumen are remarkably improved, and the fermented rapeseed meal is good in palatability, can be used as a good soybean meal substitute and has a good application prospect.

The invention belongs to the technical field of edible mushroom bran feed, and particularly relates to a method for reducing the ash content of pleurotus eryngii mushroom bran feed, which comprises the following steps: S1, preparing a mushroom bran fermentation base material; s2, activating the fermentation inoculant; s3, mixed fermentation: inoculating the activated fermentation inoculant into the mushroom bran fermentation base material, uniformly mixing, filling into a breathing fermentation bag, compacting, exhausting air in the bag, sealing, and fermenting at room temperature for 45 days to obtain the pleurotus eryngii mushroom bran fermentation feed. The pleurotus eryngii mushroom bran feed is fermented by adopting a mixed microbial agent of bacillus subtilis, candida utilis, lactobacillus plantarum and aspergillus niger, the change condition of the ash content ratio in the fermented pleurotus eryngii mushroom bran is studied, and it is found that the ash content in the pleurotus eryngii mushroom bran feed can be remarkably reduced; edible mushroom bran can be reasonably and fully utilized as an unconventional feed resource, and the method has important significance in solving the problem of feed shortage in the livestock and poultry breeding process, promoting healthy development of the breeding industry, saving resources and protecting the environment.

The invention relates to the technical field of bioengineering fermentation, and discloses a method for synergistically fermenting low-salt raw thick broad-bean sauce by fusing Weissella and Candida angustifolia, and the Weissella and Candida angustifolia are inoculated and fused to strengthen the fermentation function of the low-salt raw thick broad-bean sauce. The salinity of the low-salt raw thick broad-bean sauce prepared by fermentation with the method can be reduced to 8-10%, titratable acid is well controlled, and the color, flavor and typicality of the thick broad-bean sauce are all improved.

The invention provides plant fermented oil with moistening and soothing effects and a preparation method thereof. The preparation method comprises the following steps: fermenting aspergillus in a fermentation culture medium 1, adding candida albicans, inoculating into a fermentation culture medium 2, carrying out primary fermentation under the ventilation capacity of 2-4 L / min, carrying out secondary fermentation under the ventilation capacity of 0.2-0.5 L / min, separating, and sterilizing to obtain the plant fermented oil. The aspergillus and candida mellifera are mixed and fermented according to a specific sequence, so that the obtained plant fermented oil has excellent soothing and moistening effects. Besides, compared with products obtained through fermentation in other modes, the plant fermentation oil has more excellent soothing and moistening effects and is very suitable for being used as and / or used for preparing cosmetics or medical compositions.

This invention relates to a protein feed prepared from biorefined baijiu lees and its preparation method, belonging to the fields of bioengineering and feed resource development technology. It includes the following steps: (1) subjecting the baijiu lees to acid pretreatment and dual-enzyme synergistic hydrolysis sequentially to obtain a hydrolysate containing fermentable sugars; (2) adding Candida utilis culture and urea for carbon-nitrogen synergistic liquid fermentation to obtain a Candida utilis viable count ≥4.0 × 10⁻⁶. 8 Fermentation broth with CFU / mL; drying is then performed. This invention pretreats baijiu (Chinese liquor) lees under high pressure with dilute acid, followed by synergistic hydrolysis with two enzymes, and then utilizes *Candida utilis* for carbon-nitrogen synergistic liquid fermentation, achieving efficient utilization of glucose and xylose, with a cell protein yield ≥0.70 g / L / h. This invention realizes the high-value utilization of all components of baijiu lees, providing a process route with industrial-scale production potential for the biorefining of baijiu lees to prepare protein feed.

This invention belongs to the field of alcoholic beverage processing technology, specifically relating to a high-purity ginkgo wine and its preparation method. The preparation method comprises the following steps: ginkgo slurry undergoes preliminary enzymatic hydrolysis with neutral protease and amylase, followed by secondary enzymatic hydrolysis with pectinase and sodium bisulfite; brewing yeast fermentation broth is added for pre-fermentation; glutamine transaminase, *Saccharomyces cerevisiae* fermentation broth, and *Candida valerate* fermentation broth are added for post-fermentation; and finally, distillation and filtration are performed to obtain the ginkgo wine. This invention achieves complete decomposition and fermentation of ginkgo kernels through a two-step enzymatic hydrolysis and two-step fermentation process. The preparation method has advantages such as simple process and low cost, which is beneficial for industrial production; the prepared ginkgo wine has advantages such as clarity, strong aroma, high alcohol content, and low acidity.

The invention belongs to the technical field of microorganisms, and particularly relates to a compound fermentation inoculant for increasing the relative content of ethyl decanoate in roxburgh rose fruit wine and application. According to the present invention, two saccharomycetes are separated from a wild rosa roxburghii fermentation broth, i.e., a Starmerella bacillus and a Saccharomyces cerevisiae, and are compounded to obtain the composite fermentation bacterial agent, and the bacterial agent can produce ethyl decanoate at high yield and is applied to rosa roxburghii fruit wine brewing, such that the relative content of ethyl decanoate in the rosa roxburghii fruit wine is increased, and the yield of the rosa roxburghii fruit wine is increased; the flavor quality of the roxburgh rose fruit wine is improved, and technical support is provided for industrial development of the roxburgh rose fruit wine. When the composite microbial agent is used for fermenting the rosa roxburghii tratt fruit wine, the flavor and taste of the rosa roxburghii tratt fruit wine can be remarkably improved, the relative content of ester substances in volatile flavor substances of the rosa roxburghii tratt fruit wine is increased by 68.527% in total, and the highest relative content of ethyl decanoate is 26.551%. In addition, the relative content of ethyl laurate and the relative content of ethyl caprylate are increased to 14.228% and 11.335% respectively.

The present disclosure provides a method for producing chemicals using halotolerant yeast. The method includes culturing halotolerant yeast cells in a medium comprising a solution of salts at a concentration of at least 0.5 times the salinity of seawater, and allowing the halotolerant yeast cells to produce one or more chemicals. The medium may include a carbon source, nitrogen source and / or phosphate source, trace elements, and additives. The halotolerant yeast cells may be selected from, e.g., Debaryomyces subglobosus, Candida taylori, Pichia anomaly Millerozyma farinosa, Blastobotrys adeninivorans, Scheffersomyces stipitis. or combinations thereof. The method enables chemical production in high-salt media, reducing freshwater usage in fermentation processes.

The invention relates to the technical field of microorganisms, in particular to Devodia sp. And a method for fermenting corn steep liquor by using the Devodia sp. The invention provides the Deworth's bacteria capable of efficiently degrading vomitoxin in the corn steep liquor, the Candida krusei and the Candida krusei are used for cooperatively fermenting the corn steep liquor, the pH of the corn steep liquor can be increased, the content of vomitoxin and antinutritional factors in the corn steep liquor can be reduced, the content of choline in the corn steep liquor is remarkably increased, the quality of the corn steep liquor is obviously improved, and the yield of the corn steep liquor is increased. The corn steep liquor subjected to synergistic fermentation can be better applied to the field of animal feed and has a wide application prospect.

The present invention discloses an antioxidant composition, a preparation method thereof, a cosmetic, and an application. The antioxidant composition comprises the following components by weight: 0.1 - 2 parts of glutathione disulfide, 1 - 8 parts of olive extract, 0.1 - 2 parts of taurine sulfonate, 1 - 5 parts of Candida utilis / glucose / methyl rapeseedate fermentation product, 5 - 10 parts of vegetable oil, 20 - 50 parts of polyol, and 23 - 72.8 parts of water. The antioxidant composition of the present invention can be stored for a long time, is not easily discolored or inactivated, has significant antioxidant and whitening effects, and has a significant inhibitory effect on superoxide anion, hydroxyl radical, DHHP, and ROS. It also has excellent antioxidant stability and can be used as an additive in skin care products.

The invention relates to the technical field of microbial fermentation, in particular to a preparation method and application of a digestible biological protein fermentation material. According to the fermentation method, candida utilis serves as a fermentation strain, industrial and agricultural waste glucose mother liquor and corn steep liquor serve as substrates, high-yield mycoprotein is obtained under the relative low-temperature fermentation condition of 20-25 DEG C, and the obtained fermentation liquor is subjected to single enzymolysis, so that a fermentation material easier to digest is obtained. When weaned piglets are fed with the obtained fermented material, the diarrhea rate of the piglets can be effectively reduced.

The present invention belongs to the field of food processing technology, and discloses a kind of rice bran fresh wet noodles and preparation method thereof. According to mass percentage, the raw material components of rice bran fresh wet noodles are rice bran 25-30%, chia seed powder 3-6%, white tea powder 4-7%, wheat flour 55-65%, salt 0.5-1.5%, a first composite bacterial agent 1.5-2%, and a second composite bacterial agent 1-1.5%; wherein, the first composite bacterial agent is obtained by mixing Lactobacillus acidophilus, Lactobacillus fermentum, and Candida utilis in a mass ratio of 3-6:8-12:2-4; the second composite bacterial agent is obtained by mixing Saccharomyces cerevisiae and Lactobacillus plantarum in a mass ratio of 1-4:3-10. The rice bran fresh wet noodles of the present invention are bright in color and light yellow. Compared with the existing fresh wet noodles, the nutrient content is significantly increased. It is not easy to break and will not become mushy during cooking. It has a more delicate and smooth taste, good viscoelasticity and chewiness, and shows better quality.

The invention belongs to the technical field of food processing, and discloses fine broken rice wet noodles and a preparation method thereof. The fresh wet fine broken rice noodles comprise the following raw material components in percentage by mass: 25-30% of fine broken rice, 3-6% of chia seed powder, 4-7% of white tea powder, 55-65% of wheat flour, 0.5-1.5% of table salt, 1.5-2% of a first complex microbial inoculant and 1-1.5% of a second complex microbial inoculant, wherein the first complex microbial inoculant is obtained by mixing lactobacillus acidophilus, lactobacillus fermentum and candida utilis according to the mass ratio of (3-6): (8-12): (2-4); the second complex microbial inoculant is obtained by mixing saccharomyces cerevisiae and lactobacillus plantarum according to the mass ratio of (1-4): (3-10). The fine broken rice fresh wet noodles are bright in color and light yellow, and compared with existing fresh wet noodles, the fine broken rice fresh wet noodles are obviously increased in nutrient substance content, not prone to breakage during cooking, free of soup burning, finer and smoother in taste, better in viscoelasticity and chewiness and better in quality.

The invention discloses a method for preparing xylitol and L-arabinose from liquid xylitol, comprising the following steps: (1) diluting a xylose mother liquor, adding a nitrogen source and an inorganic salt, sterilizing, and setting aside; (2) inoculating Candida for fermentation, wherein the Candida can decompose other sugars except arabinose; (3) after the fermentation is completed, centrifuging and filtering the fermentation liquid to obtain a supernatant; (4) desalting the supernatant by passing an ion exchange resin; (5) removing pigment from the supernatant, concentrating and crystallizing it, and separating the crystallized material to obtain xylitol crystals and a xylitol mother liquor; (6) separating the obtained xylitol mother liquor by a chromatographic system to obtain a xylitol phase and an L-arabinose phase; (7) concentrating and crystallizing the obtained L-arabinose phase, and separating the crystallized material to obtain L-arabinose crystals and an L-arabinose liquid. The method of the invention can obtain xylitol with a higher yield, and separate high-purity and high-yield L-arabinose from it.

The present application relates to high protein fermentation technical field, specifically disclose a kind of preparation method for fermenting production of yeast protein based on alcohol waste liquor, to solve the technical problems such as low utilization rate of alcohol waste liquor resource, feed soybean meal supply is nervous and high cost.The method includes the following steps: first, the alcohol waste liquor produced by grain deep processing is concentrated to dry basis content ≥30%, the composite carbon source carrier is crushed, urea and molasses are added to adjust the carbon-nitrogen ratio to (20~25):1, access to the complex fermentation strain containing Candida utilis, solid fermentation is carried out, and the finished product yeast protein is obtained.The present application uses solid fermentation mode, matches composite carbon source carrier to optimize nutrient ratio, step by step controls key process parameters, and the finished product yeast protein is ≥24%, true protein is ≥22%, and nitrogen conversion rate is ≥85%, which can replace soybean meal as feed additive.The method realizes alcohol waste liquor resource utilization, pollution is small, reduces feeding cost, has environmental protection and economic value, and is suitable for industrialization popularization and application.

According to the tomato pomace fermented protein feed and the preparation method thereof provided by the invention, tomato pomace and bran are used as fermentation substrates, candida utilis and pichia pastoris are used as fermentation strains, fermentation processes such as strain inoculum size, fermentation time and enzyme addition amount are further optimized, and under optimal fermentation process conditions, the tomato pomace fermented protein feed is prepared. The content of crude protein in the obtained tomato peel residues is 35.93%. After fermentation, the crude protein content of the tomato peel residues is increased by 10.59%, and hemicellulose is reduced by 4.81%. The tomato pomace is cooperatively fermented by bacteria and enzymes, so that the cellulose content of the tomato pomace is reduced, the protein content of the tomato pomace is increased, the tomato pomace can be subsequently used for feeding monogastric livestock and poultry, and the method has great significance in realizing resource utilization of wastes, reducing environmental pollution and relieving waste treatment pressure faced by tomato processing enterprises. The nutritional value of the feed can be improved, the breeding cost is reduced, and sustainable development of animal husbandry is promoted.

The present invention relates to the technical field of microbial fermentation, and discloses a method for producing selenium-rich protein feed by multi-strain co-fermentation of Hami melons. The Hami melons are crushed, then mixed with a carbon source and a nitrogen source, and then inoculated with a fermentation agent for fermentation; the fermented product is dried and pulverized to obtain a protein feed; the fermentation agent includes Candida utilis, Aspergillus niger, and Bacillus licheniformis; the components of the Candida utilis culture medium include yeast extract 9.56 g / L, glucose 19.86 g / L, dipotassium hydrogen phosphate 1.45 g / L, magnesium sulfate 1.12 g / L, sodium selenite 25 mg / L, L-lysine and vitamin B1. In terms of mass concentration, the ratio of sodium selenite, L-lysine and vitamin B1 is 1:(116-232):(0.06-0.12). In the present invention, multi-strain co-fermentation of Hami melons is adopted to obtain a selenium-rich protein feed.

The present invention relates to the technical field of skin care products, and specifically discloses a composition containing superoxide dismutase (SOD) and a preparation method thereof. The composition containing superoxide dismutase (SOD) comprises the following components: 28-32 parts of yeast fermentation product filtrate; 0.8-1.2 parts of garlic bulb extract; 0.8-1.2 parts of superoxide dismutase (SOD); 0.4-0.6 parts of angelica extract; 65-70 parts of water; the preparation method of the yeast fermentation product filtrate is as follows: 1), mix broom, hops, hibiscus bark, and perilla leaves evenly to obtain a plant mixture; 2), mix the plant mixture with water evenly to obtain a plant mixture; 3), inoculate bumblebee Candida albicans and Cryptococcus laurentii into the plant mixture, ferment for 7-9 days, filter, and sterilize to obtain a yeast fermentation product filtrate. The present invention has the advantages of improving the anti-wrinkle and firming effect to achieve rapid wrinkle reduction.

The invention belongs to the crossing field of cultivation and bioengineering, and particularly relates to a multi-strain insect protein and microalgae compounded bullfrog feed and a preparation method thereof, the multi-strain insect protein and microalgae compounded bullfrog feed comprises the following components: 25-45% of fermented insect protein, 15-30% of DHA microalgae powder, 10-20% of an auxiliary protein source, 0.5-2% of compound vitamin selected from fermented soybean meal or enzymolysis cottonseed protein, 0.5-2% of vitamin B, 0.5-2% of vitamin C, 0.5-2% of vitamin C, 0.5-2% of vitamin C, 0.5 1%-3% of composite mineral substances, 2%-5% of an adhesive and the balance of carbohydrate; the total DHA content of the bullfrog feed is larger than or equal to 1.5% by dry weight, the ratio of omega-3 fatty acid to omega-6 fatty acid is maintained to be 1: 1-1: 3, and the bullfrog feed has the advantages that saccharomyces cerevisiae is replaced with candida utilis, so that the capability of decomposing insect chitin is enhanced; chlorella is used for supplementing chlorophyll and algal polysaccharides to form a cell protection synergistic effect with schizochytrium limacinum, and DHA is supplemented at the same time; under the action of alkaline protease secreted by bacillus subtilis, the proportion of a beta-folding structure is reduced from 32% to 18%, and the digestibility is remarkably improved.

The invention provides a method for preparing ruminant biological feed through fermentation of sodium humate and probiotics, and relates to the field of solid feed fermention.The method comprises the steps that S1, candida utilis powder, lactobacillus plantarum liquid, enterococcus faecium liquid and beet molasses are stirred and mixed, and molasses bacterial liquid is obtained; s2, mixing the molasses bacterial liquid with the treated sodium humate and gelatinized starch urea according to a preparation ratio, and uniformly stirring; and S3, loading into an anaerobic sealed fermentation bag, and naturally fermenting in a greenhouse at the temperature of 25-35 DEG C for 30-48 hours. The biological feed which is rich in high-activity probiotics, high in protein and easy to digest and absorb by animals is prepared from byproducts such as sodium humate, molasses and the like by utilizing biological engineering, and can replace antibiotics in the feed.