The invention discloses a culturing method of a 
gerbera jamesonii novel variety. The method comprises the steps that after lateral buds of the 
gerbera jamesonii novel variety are taken and sterilized,
bud tip parts are taken to be grafted to a culture medium, and culturing is conducted to obtain a 
callus; the 
callus is grafted to a propagation culture medium to by cultured to obtain a clump 
shoot;after the clump 
shoot is segmented, the clump 
shoot is grafted to a rooting culture medium, and a rooting 
seedling is obtained; the rooting 
seedling is hardened and taken out, sterilized and transplanted to a nutritious culture medium to be cultured, and a 
gerbera jamesonii novel variety young 
seedling is obtained; the propagation culturing medium contains 
sodium p-toluenesulfonate and 
ethyl decanoate. The invention provides a culturing method of the 
gerbera jamesonii novel variety, the method is simple, the implementation is easy, the 
operability is high, the 
repeatability is high, the emergence rate of 
vitrification seedlings in tissue culturing is reduced, the culturing efficiency is effectively improved, the purposes of efficient and rapid propagation are achieved, and the 
gerbera jamesonii novel variety young seedling has the advantages of stable hereditary character, low variation, excellent quality, strong 
root system and the increasing number of 
tiller.