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31 results about "Ethyl decanoate" patented technology

Ethyl decanoate, also known as ethyl caprate, is a fatty acid ester formed from capric acid and ethanol. This ester is a frequent product of fermentation during winemaking, especially at temperatures above 15 °C.

Saccharomyces cerevisiae highly producing C6-C10 ethyl esters and construction method and purpose of saccharomyces cerevisiae

The invention discloses saccharomyces cerevisiae highly producing C6-C10 ethyl esters and a construction method and purpose of the saccharomyces cerevisiae, and belongs to the technical field of bioengineering. Through overexpressing acetaldehyde dehydrogenase ALD6 and acetyl-CoA synthase ASC1 in an original strain, synthesis of acetyl-CoA is strengthened, through overexpressing acetyl-CoA carboxylase ACC1**, malonyl CoA is strengthened, through overexpressing fatty acid synthetase FAS1 and fatty acid synthetase FAS2, medium-chain acyl-CoA is strengthened, more metabolism flows flow to medium-chain acyl groups CoA, alcohol acyl-transferase genes SAAT in strawberries are further exogenously introduced, and a reformed strain C-ald6acs1A*F1F2S is obtained. Under the condition of fermentation,the yield of ethyl caproate is 7.53mg/L which is 2.72 times of that of an original strain C-ald6acs1A*F1F2, and 26.89 times of that (only 0.27mg/L) of an original strain CA, the yield of ethyl caprylate is 13.65mg/L which is 9.11 times of that of the original strain CA, the yield of ethyl decanoate is 13.89mg/L which is 7.27 times of that of the original strain, and the saccharomyces cerevisiae has potential application prospects in improving flavor of wine and improving the quality of the wine.
Owner:TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY

Culture method of burkholderia multivorans strain and applications of burkholderia multivorans in catalyzing synthesis of baijiu flavor esters and degradation of baijiu harmful esters

The present invention belongs to the technical field of microorganisms and particularly relates to a culture method of burkholderia multivorans CGMCCCY 1.3829 and applications of burkholderia multivorans in catalyzing synthesis of baijiu flavor esters and degradation of baijiu harmful esters. The bacterium cultured by the method has high ability to catalyze the synthesis of the baijiu flavor esters of ethyl hexanoate, ethyl octoate, and ethyl decanoate in water phase systems. Experiments show that yield of the ethyl hexanoate can reach 61.90 plus or minus 8.04 mg/L, yield of the ethyl octoatecan reach 746.27 plus or minus 82.45 mg/L and yield of the ethyl decanoate can reach 783.91 plus or minus 76.60 mg/L, respectively. At the same time, according to the culture method, the burkholderiamultivorans also has ability to degrade the baijiu harmful esters of dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP) and di-(2-ethylhexyl) phthalate (DEHP). Experiments showthat a degradation rate of the dimethyl phthalate can reach 19.07% plus or minus 0.58%, a degradation rate of the diethyl phthalate can reach 18.95% plus or minus 0.41%, a degradation rate of the dibutyl phthalate can reach 36.33% plus or minus 16.85%, and a degradation rate of the di-(2-ethylhexyl) phthalate can reach 53.76% plus or minus 17.60%.
Owner:BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY

Culture method of burkholderia cepacia and application thereof in catalytic synthesis of flavor esters of baijiu and degradation of harmful esters of baijiu

The invention belongs to the technical field of microbes and particularly discloses a culture method of burkholderia cepacia CGMCC1.3055 and application thereof in catalytic synthesis of a flavor esters of baijiu and degradation of harmful esters of baijiu. The burkholderia cepacia CGMCC1.3055 has the capacity of efficiently catalyzing and synthesizing baijiu flavor esters ethyl butyrate, ethyl valerate, ethyl hexanoate, ethyl octanoate and ethyl decanoate in a water phase system and degrading harmful baijiu esters dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP) andphthalic acid bis(2-ethylhexanoic) ester (DEHP), the yields of the flavor esters are 4.26+ / -1.21, 14.42+ / -2.42, 148.91+ / -15.60, 1331.34+ / -75.33 and 1610.89+ / -90.31 mg / L respectively, and the degradation rates of the harmful esters are 18.56%+ / -1.46%, 20.62%+ / -1.00%, 21.76%+ / -2.89% and 37.52%+ / -4.65% respectively.
Owner:BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY

Liquid concentrate for seasoning, wood piece usable as fermentation index for seasoning, kit for manufacturing seasoning, method for manufacturing seasoning, seasoning, and thickened seasoning

The first purpose of the present invention is to provide: a liquid concentrate for a seasoning, said liquid concentrate being usable in easily performing yeast fermentation even at home so as to givea highly preferable seasoning with a rich aroma; a fermentation control index; a set of members including the liquid concentrate and the index; and a method for manufacturing a seasoning. The second purpose of the present invention is to provide a seasoning which sustains the inherent flavor of soy sauce and yet has a fruity aroma scarcely noticeable in conventional soy sauces. The first purpose can be achieved by: a liquid concentrate for a seasoning, said liquid concentrate having a reducing sugar content of 4.5 wt% or more and an ethanol content of 1.5 wt% or less; a compressed wood piece characterized by having a volume less than 20 cm3; a kit for manufacturing a seasoning, said kit including the liquid concentrate, the wood piece, a yeast-containing solution and a container for yeastfermentation; and a method for manufacturing a seasoning with the use of the kit. The second purpose can be achieved by a soy sauce, a soy sauce-like seasoning, etc., containing ethyl octanoate and / orethyl decanoate.
Owner:KIKKOMAN CORP

Phlomis betonicoides rhizome essence for cigarette blasting beads, blasting bead prepared from the same and cigarette

The invention relates to a phlomis betonicoides rhizome essence for cigarette blasting beads, blasting beads prepared from the same and a cigarette, and belongs to the technical field of tobacco essence. The phlomis betonicoides rhizome essence comprises a phlomis betonicoides rhizome extract, a loquat extract, a snow pear extract, ethyl vanillin, perillartine, 2-methylbutyric acid, 2-methylbutyric acid-2-phenethyl ester, 2, 3, 5-trimethylpyrazine, sweet orange oil terpene, phenethyl acetate, caprolactone, ethyl maltol, isoamyl acetate, isovaleric acid, butyl butyrate, ethyl caprate, ethyl heptanoate, beta-caryophyllene and the like. According to the invention, the nature, taste and efficacy of phlomis betonicoides rhizome are applied to the tobacco blasting bead essence, the tobacco blasting bead with the effect of relieving throat discomfort is developed, the smoking comfort of cigarettes is improved through the blasting bead, the smoking sweetness and moistening feeling of cigarettes are improved, the piercing and choking feeling in cigarette smoke is reduced, the effect of engendering liquid is achieved, and the phlomis betonicoides rhizome essence has high application value and popularization significance.
Owner:CHINA TOBACCO YUNNAN IND

Saccharomyces cerevisiae engineering bacterium for highly yielding medium-chain fatty acid ethyl ester as well as construction method thereof

ActiveCN103571764BIncrease contentFungiMicroorganism based processesFatty acid activating enzymeBacterial strain
The invention provides a saccharomyces cerevisiae engineering bacterium for highly yielding medium-chain fatty acid ethyl ester. The saccharomyces cerevisiae engineering bacterium is realized by selecting a strong promoter PGK1 (Phosphoglycerate kinase 1) for overexpression coding of an EHT1 (Ethanol Hexanoyl Transferase 1) gene of alcohol acyltransferase and knocking out a gene FFA1 (Free Fatty Acid Receptor 1) of an exogenous fatty acid activating enzyme. The preservation number is CGMCC (China General Microbiological Culture Collection Center) No.7937. Under the condition that other fermenting properties are not affected, compared with a parent bacterial strain, the content of ethyl hexanoate can be improved to 2.23mg / L after simulating fermentation of corn raw material liquid white spirit for 15 days, wherein the content is 2.75 times the original bacteria. The contents of ethyl caprylate and ethyl caprate are respectively improved by 52% and 62%. After fermentation for 30 days, the contents of ethyl hexanoate, ethyl caprylate and ethyl caprate are respectively improved by 120%, 16.2% and 16.7%. After simulating fermentation of corn raw material liquid white spirit for 15 days, the content of ethyl hexanoate can be improved to 2.83mg / L which is 2.8 times the original bacteria, and the contents of ethyl caprylate and ethyl caprate are respectively improved by 43.3% and 40.9%.
Owner:TIANJIN UNIV OF SCI & TECH

A kind of cultivation method of new gerbera chrysanthemum variety

The invention discloses a culturing method of a gerbera jamesonii novel variety. The method comprises the steps that after lateral buds of the gerbera jamesonii novel variety are taken and sterilized,bud tip parts are taken to be grafted to a culture medium, and culturing is conducted to obtain a callus; the callus is grafted to a propagation culture medium to by cultured to obtain a clump shoot;after the clump shoot is segmented, the clump shoot is grafted to a rooting culture medium, and a rooting seedling is obtained; the rooting seedling is hardened and taken out, sterilized and transplanted to a nutritious culture medium to be cultured, and a gerbera jamesonii novel variety young seedling is obtained; the propagation culturing medium contains sodium p-toluenesulfonate and ethyl decanoate. The invention provides a culturing method of the gerbera jamesonii novel variety, the method is simple, the implementation is easy, the operability is high, the repeatability is high, the emergence rate of vitrification seedlings in tissue culturing is reduced, the culturing efficiency is effectively improved, the purposes of efficient and rapid propagation are achieved, and the gerbera jamesonii novel variety young seedling has the advantages of stable hereditary character, low variation, excellent quality, strong root system and the increasing number of tiller.
Owner:云南喜迎春科技有限公司

Artificial induced spawning method for loach cultivation

The invention provides an artificial induced spawning method for loach culture, and belongs to the field of artificial induced spawning; the method comprises a premature induced spawning process of sexual maturity of parent loaches, an artificial induced spawning process and a fertilized egg hatching process; the fertilized egg hatching process comprises the following steps: placing the fertilizedegg into an extreme temperature or an extreme acidification environment for intervention, and culturing and hatching the fertilized egg; in the prophase maturation promoting process of the sexual maturity of the loach, using the traditional Chinese medicine extract dispersed in the matrix as a gonadal maturation promoting medicament, wherein the traditional Chinese medicine extract is prepared bymagnetizing and leaching traditional Chinese medicine raw materials in the presence of a magnetizing medium containing laurocapram and ethyl decanoate. The artificial induced spawning method for loach culture has the advantages of high efficiency, short spawning and hatching period, high biological activity of the fertilized eggs, low adhesion, no pollution by external germs, high loach seedlingoutput and survival rate and high uniformity; the gonad maturation promoting agent can reduce loach excretion, reduce water body pollution, increase spawning number, improve fertilized egg quality, and reduce death and death amount of parent loach after spawning.
Owner:佛山市嘉沃农业科技合伙企业(有限合伙)

Liquid concentrate for seasoning, wood piece usable as fermentation index for seasoning, kit for manufacturing seasoning, method for manufacturing seasoning, seasoning, and thickened seasoning

A first object of the present invention is to provide a raw liquid for a seasoning which can be utilized for obtaining a seasoning with a mellow aroma and high palatability by simply performing yeast fermentation so as to enable the yeast fermentation even at home, an index of fermentation control, a set of members including the raw liquid and the index, and a method for manufacturing a seasoning. A second object of the present invention is to provide a seasoning that has a fruity aroma hardly noticeable in a conventional soy sauce while having a flavor inherent in a soy sauce. The first object is achieved by a raw liquid for a seasoning having a reducing sugar content of 4.5 wt % or more and an ethanol content of 1.5 wt % or less, a wood piece characterized by having been subjected to compression processing, and having a volume less than 20 cm3, a kit for manufacturing a seasoning including the raw liquid, the wood piece, a yeast-containing liquid, and a container for yeast fermentation, and a method for manufacturing a seasoning utilizing the kit. The second object is achieved by a soy sauce or a soy sauce-like seasoning containing ethyl octanoate and / or ethyl decanoate, or the like.
Owner:KIKKOMAN CORP
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