Artificial induced spawning method for loach cultivation
A technology of artificial induction of labor and loach, applied in fish farming, application, climate change adaptation and other directions, can solve the problems of fertilized egg adhesion, reduce the hatching rate of fertilized eggs, prolong the breeding cycle, etc., achieve good growth and development, shorten incubation time, The effect of reducing casualties
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Embodiment 1
[0033] The artificial induced spawning method of loach breeding includes the pre-maturity promoting process of parental loach, the artificial induced spawning process, and the fertilized egg hatching process; the fertilized egg hatching process is to intervene in an extreme environment before cultivating and hatching the fertilized eggs. This method of oxytocin takes advantage of the high sensitivity of fertilized eggs to the living environment. When the cells are stimulated by the external environment, they can quickly synthesize protective substances with self-repairing properties to resist these adverse conditions, so that the fertilized eggs can accelerate their initiation. During the period, a higher film production rate, embryo survival rate and hatching rate are obtained, and production costs are saved to meet the large demand for loach seedlings in artificial breeding.
[0034] The extreme environment is an extreme temperature environment, and the specific conditions ar...
Embodiment 2
[0051] Loach culture artificial induced spawning method specifically comprises the following steps:
[0052]1) Prepare a cultivation pond with an overflow device at the bottom, then inject water into the pond and disinfect it for 3 hours, then inject water again, and keep the water temperature in the pond constant at 25°C. The above cultivation pond is disinfected by splashing the whole pond with a mixture of copper sulfate and ferrous sulfate , the dosage of copper sulfate is 1.2g / m 3 , the proportion of copper sulfate and ferrous sulfate is 4.5:2.5, and the above-mentioned overflow device is set as follows: at the bottom of the cultivation pool, the diameter of the upper hole is first laid at the bottom of the pond, and the diameter of the upper hole is 150 meshes, and then the diameter of the mesh is 20 meshes. The edges of both the dense net and the thin net will extend to the edge of the cultivation pool;
[0053] 2) Select a parent loach with a body length of 17cm, a bo...
Embodiment 3
[0060] The method of artificially induced spawning in loach breeding, optimizes the debonding treatment steps, and the specific measures are as follows: When the yield of loach reaches 85%, pull up the thin net in the hatching tank, so that the fertilized eggs of loach can leak to the dense net below in time , then place 4 times the weight of fertilized eggs in the debonding suspension and stir until all the fertilized eggs are in the form of dispersed particles. Suspension prepared by mixing the acid with a weight ratio of 1:0.08:0.04. Since loach eggs are sticky eggs and sinking eggs, after natural mating, the fertilized eggs are easily bonded together. Add to the debonding agent After dextrorotary 4-methyl-2-pentanol and abscisic acid, highly active α and β unsaturated ketone structures and dextro-isomers work together to combine with hydrogen atoms of amino acids in proteins on the fertilized egg cell membrane, thereby generating stimulation It can inhibit the secretion of...
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